Integrated device for nucleic acid extraction and nucleic acid methylation

Abstract

The utility model provides an integrated device for nucleic acid extraction and nucleic acid methylation, which comprises a bottom plate and a magnetic adsorption mechanism, and the magnetic adsorption mechanism comprises a magnetic adsorption assembly for adsorbing magnetic beads in a reaction tube and a driving assembly for driving the magnetic adsorption assembly to lift, the driving assembly comprises a lead screw in threaded connection with the magnetic attraction assembly and a rotary driving motor, an output rotating shaft of the rotary driving motor is in transmission connection with the driving end of the lead screw through a synchronous belt, and the rotating axis of the lead screw and the rotating axis of the output rotating shaft are arranged in parallel in a spaced mode. The lead screw and the rotary driving motor are projected on a vertical plane in the direction perpendicular to the output rotating shaft, and the axial length of the rotary driving motor is within the axial length range of the lead screw. The overall design height of the integrated device can be effectively reduced, and the structure of the device is more compact.

Description

Technical Field

[0001] The utility model belongs to the technical field of biological sample processing equipment, and particularly relates to an integrated device for nucleic acid extraction and nucleic acid methylation. Background Art

[0002] Methylation refers to the process of catalytically transferring a methyl group from an active methyl compound to other compounds, which can form various methyl compounds, or chemically modify certain proteins or nucleic acids to form methylation products. In biological systems, methylation is enzyme-catalyzed, and this methylation involves heavy metal modification, regulation of gene expression, regulation of protein function, and ribonucleic acid processing. In recent years, DNA methylation detection has gradually become a research hotspot. The current traditional operation is to first use an automated nucleic acid extraction device for automated nucleic acid extraction to reduce manual operation, avoid human contamination, and improve experimental efficiency. After nucleic acid extraction, corresponding methylation conversion operations are carried out. This method still involves manual participation and has the problem of human contamination. Based on the above, the utility model patent with the publication number of CN221166553 U discloses a nucleic acid extractor, which realizes the whole process automation from nucleic acid extraction to methylation conversion, can reduce manual operation, avoid human contamination, and save experimental time. However, the overall height of the magnetic adsorption mechanism in this patent is relatively large, resulting in an unreasonable layout of the internal components of the device, and thus the overall volume of the device is relatively large. Summary of the Utility Model

[0003] Therefore, the technical problem to be solved by the utility model is to provide an integrated device for nucleic acid extraction and nucleic acid methylation, which can optimize the layout of the internal components of the device through the transmission drive design of the drive motor of the magnetic adsorption mechanism, and ensure that the overall size of the device is more compact.

[0004] To solve the above problems, the utility model provides an integrated device for nucleic acid extraction and nucleic acid methylation, including a bottom plate and a magnetic adsorption mechanism. The magnetic adsorption mechanism includes a magnetic adsorption component for adsorbing magnetic beads in a reaction tube and a drive component for driving the magnetic adsorption component to lift. The drive component includes a lead screw threadedly connected to the magnetic adsorption component and a rotary drive motor. The output rotating shaft of the rotary drive motor is connected to the drive end of the lead screw through a synchronous belt drive. The rotation axis of the lead screw is parallel and spaced from the rotation axis of the output rotating shaft. Project the lead screw and the rotary drive motor on a vertical plane along the direction perpendicular to the output rotating shaft. The axial length of the rotary drive motor is within the axial length range of the lead screw.

[0005] In some embodiments, the end face of the free end of the output rotating shaft and the end face of the drive end of the lead screw are on the same horizontal plane.

[0006] In some embodiments, a driving wheel is sleeved on the free end of the output rotating shaft, a driven wheel is sleeved on the driving end of the lead screw, and the synchronous belt is tensioned between the driving wheel and the driven wheel.

[0007] In some embodiments, the integrated device for nucleic acid extraction and nucleic acid methylation further includes a reaction platform for placing reagent kits, sample tubes and finished product tubes. The reaction platform is located in the top area of the bottom plate and supported on the bottom plate. The rotary drive motor is assembled above the top surface of the bottom plate and is located in the gap space formed between the reagent kit and the bottom plate.

[0008] In some embodiments, the magnetic adsorption mechanism further includes a guiding component. The guiding component includes two vertical optical rods. The two vertical optical rods are slidably connected to the magnetic adsorption component through sliding bearings. The bottom ends of the vertical optical rods are fixedly connected to the bottom plate, and the top ends of the vertical optical rods are fixedly connected to the reaction platform.

[0009] In some embodiments, the magnetic adsorption component includes an assembly plate and a plurality of magnets assembled on one side surface of the assembly plate. Each magnet is uniformly spaced along the length extension direction of the assembly plate and is arranged in one-to-one correspondence with the reaction tubes of the reagent kit. The sliding bearing is integrally connected to the assembly plate, and the lead screw is threadedly connected to the assembly plate.

[0010] In some embodiments, a plurality of spaced and parallel first accommodating grooves are formed on the reaction platform. The first accommodating grooves extend along a first direction. Each reagent kit is respectively accommodated in each first accommodating groove. A second accommodating groove for accommodating the sample tube and a third accommodating groove for accommodating the finished product tube are further formed on the reaction platform. The second accommodating groove and the third accommodating groove both extend along a second direction. The second direction is perpendicular to the first direction, and the number of tube holes on the sample tube and the finished product tube is equal to the number of the first accommodating grooves.

[0011] In some embodiments, a fourth accommodating groove for accommodating the waste liquid tube is further formed on the reaction platform between the second accommodating groove and the third accommodating groove. The fourth accommodating groove extends along the second direction, and the number of tube holes on the waste liquid tube is equal to the number of the first accommodating grooves.

[0012] In some embodiments, holding balls are respectively arranged at both ends of the top surface of the waste liquid tube and / or the finished product tube.

[0013] An integrated device for nucleic acid extraction and nucleic acid methylation provided by the present utility model is driven and connected between a rotary drive motor and a lead screw through a synchronous belt drive, rather than directly driven and connected as in the prior art. In this way, the axial length of the rotary drive motor can at least partially coincide with the axial length of the lead screw, so that the height dimension of the drive assembly can be designed to be smaller, effectively reducing the overall design height of the integrated device and making the device structure more compact. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 FIG. 6 is a schematic perspective view of the integrated device for nucleic acid extraction and nucleic acid methylation according to an embodiment of the present utility model (the door is in an open state);

[0015] Figure 2 is Figure 1 a schematic perspective view of the structure with some parts omitted;

[0016] Figure 3 is Figure 2 a sectional view showing the positional relationship between the lead screw and the rotary drive motor;

[0017] Figure 4 is Figure 2 a schematic sectional structure view of some components of the magnetic adsorption mechanism in FIG. 18;

[0018] Figure 5 is Figure 2 a schematic perspective view (sectional) of the magnetic adsorption mechanism in FIG. 24.

[0019] The reference numerals are shown as:

[0020] 1, bottom plate; 2, magnetic adsorption mechanism; 21, magnetic attraction assembly; 211, assembly plate; 212, magnet; 221, lead screw; 222, rotary drive motor; 2221, output rotating shaft; 2222, driving wheel; 223, synchronous belt; 224, driven wheel; 231, vertical optical rod; 232, sliding bearing; 3, reaction platform; 31, reagent kit; 32, sample row tube; 33, finished product row tube; 34, waste liquid row tube; 35, hand-held ball; 41, device base assembly; 42, pipetting gun assembly; 43, power supply box assembly; 44, outer shell; 45, door. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0021] See Figures 1 to 5As shown, according to an embodiment of the present invention, an integrated device for nucleic acid extraction and nucleic acid methylation is provided, including a device base assembly 41 and a shell 44 covered thereon, an internal accommodating space is formed between the shell 44 and the device base assembly 41, and a compartment door 45 is formed on the shell 44 for opening or closing the aforementioned internal accommodating space, thereby meeting the requirements of taking and placing samples and other materials, and the various functional components of the aforementioned integrated device are assembled in the internal accommodating space; the internal accommodating space is specifically assembled with a pipette gun assembly 42 for pipetting and a power supply box assembly 43 for power supply. As a conventional structure, the specific structure is not repeated here. The internal accommodating space is further assembled with a bottom plate 1 and a magnetic adsorption mechanism 2. The bottom plate 1 is specifically assembled on the aforementioned device base assembly 41 so that the lateral displacement of the bottom plate 1 and the components assembled thereon can be driven by the lateral movement assembly on the device base assembly 41 to ensure that the aforementioned pipetting gun assembly 42 performs pipetting operations at different positions. The magnetic adsorption mechanism 2 includes a magnetic adsorption assembly 21 for adsorbing magnetic beads (not shown in the figure) in a reaction tube (located on a reagent kit 31 described later) and a driving assembly for driving the magnetic adsorption assembly 21 to rise and fall. The driving assembly includes a magnetic adsorption assembly 21 connected to the magnetic adsorption assembly 21. A screw rod 221 and a rotary drive motor 222 are threadedly connected (for example, connected to the nut thread on the magnetic attraction component 21), and the output shaft 2221 of the rotary drive motor 222 is connected to the driving end of the screw rod 221 through a synchronous belt 223. The rotation axis of the screw rod 221 is parallel to the rotation axis of the output shaft 2221 and is arranged at intervals. The screw rod 221 and the rotary drive motor 222 are projected on a vertical plane in a direction perpendicular to the output shaft 2221. The axial length of the rotary drive motor 222 is within the axial length range of the screw rod 221.

[0022] In this technical solution, the rotary drive motor 222 and the screw rod 221 are connected by a synchronous belt 223, instead of adopting the direct drive connection in the prior art. In this way, the axial length of the rotary drive motor 222 can at least partially overlap with the axial length of the screw rod 221, so that the height dimension of the drive component can be designed to be smaller, which can effectively reduce the overall design height of the integrated device and make the device structure more compact.

[0023] In some embodiments, the free end surface of the output shaft 2221 (ie Figure 3 The bottom end of the orientation shown) and the driving end of the screw rod 221 (ie Figure 3 The end faces of the bottom end (in the orientation shown) are on the same horizontal plane, so that the axial length of the rotary drive motor 222 is completely within the range of the axial length of the screw rod 221, so that the height of the device will not be increased due to the addition of the rotary drive motor 222, and the degree of compactness is further improved.

[0024] See Figure 3 As shown, a driving wheel 2222 is mounted on the free end of the output shaft 2221, and a driven wheel 224 is mounted on the driving end of the screw rod 221. The synchronous belt 223 is tensioned on the driving wheel 2222 and the driven wheel 224. The driven wheel 224 and the driving wheel 2222 are connected to the corresponding shaft in a detachable and washable manner, so that the lifting and lowering speed of the magnetic attraction component can be adjusted as necessary by replacing different wheel parts to meet different operating rhythms.

[0025] In some embodiments, the integrated device for nucleic acid extraction and nucleic acid methylation also includes a reaction platform 3 for placing a reagent kit 31, a sample tube 32 and a finished product tube 33. It can be understood that the aforementioned reagent kit 31 has multiple holes, each of which is equipped with various reagents related to nucleic acid extraction and methylation. Each hole realizes the overall operation process of nucleic acid extraction and nucleic acid methylation under the cooperation of the aforementioned pipette gun assembly 42. The reaction platform 3 is located in the top area of the base plate 1 and is supported on the base plate 1 by corresponding support columns (not marked in the figure). The rotary drive motor 222 is assembled on the top surface of the base plate 1 and is located in the gap space formed between the reagent kit 31 and the base plate 1.

[0026] In this technical solution, the rotary drive motor 222 is arranged in the gap space between the bottom plate 1 and the reagent box 31, which can fully utilize the internal space of the device without occupying the height of the device, and the layout is more reasonable and compact.

[0027] In some embodiments, the magnetic adsorption mechanism 2 also includes a guide assembly (not labeled in the figure), which includes two vertical light rods 231, and the two vertical light rods 231 are slidingly connected to the magnetic attraction assembly 21 through sliding bearings 232. The bottom ends of the vertical light rods 231 are fixedly connected to the base plate 1 through rolling bearings, and the top ends of the vertical light rods 231 are fixedly connected to the reaction platform 3 through rolling bearings.

[0028] In this technical solution, two vertical polished rods 231 are used to guide the magnetic assembly 21 in its lifting and lowering, ensuring a smoother lifting and lowering process. Specifically, the two vertical polished rods 231 are respectively arranged on both sides of the screw rod 221. This ensures smooth guidance of both ends of the magnetic assembly 21 in the longitudinal direction.

[0029] See also Figure 4 and Figure 5As shown, in some embodiments, the magnetic attraction assembly 21 includes an assembly plate 211 and a plurality of magnets 212 assembled on one side surface of the assembly plate 211. Each of the magnets 212 is arranged at uniform intervals along the length extension direction of the assembly plate 211, and is arranged in one-to-one correspondence with the positions of the reaction tubes of the reagent kit 31. The sliding bearing 232 is integrally connected with the assembly plate 211, and the lead screw 221 is threadedly connected with the assembly plate 211.

[0030] In some embodiments, a plurality of spaced and parallel first accommodation grooves (not labeled in the figure) are formed on the reaction platform 3. The first accommodation grooves extend along a first direction. Each of the reagent kits 31 is respectively accommodated in each of the first accommodation grooves. A second accommodation groove (not labeled in the figure) for accommodating the sample discharge tube 32 and a third accommodation groove (not labeled in the figure) for accommodating the finished product discharge tube 33 are further formed on the reaction platform 3. The second accommodation groove and the third accommodation groove both extend along a second direction. The second direction is perpendicular to the first direction, and the number of tube holes on the sample discharge tube 32 and the finished product discharge tube 33 is equal to the number of the first accommodation grooves. The aforementioned first direction is also Figure 1 the left-right direction in the shown orientation, and objectively, it is also the reciprocating linear motion direction in which the bottom plate 1 and each component thereon are driven by the device base assembly 41.

[0031] In this technical solution, by defining the arrangement directions of the sample discharge tube 32, the finished product discharge tube 33 and each reagent kit 31, the operation path of the aforementioned liquid transfer pipetting gun assembly 42 is optimized, and the liquid transfer efficiency is improved.

[0032] For details, refer to Figure 2 As shown, in some embodiments, a fourth accommodation groove (not labeled in the figure) for accommodating the waste liquid discharge tube 34 is further formed on the reaction platform 3 between the second accommodation groove and the third accommodation groove. The fourth accommodation groove extends along the second direction, and the number of tube holes on the waste liquid discharge tube 34 is equal to the number of the first accommodation grooves. Arranging the waste liquid discharge tube 34 between the finished product discharge tube 33 and the sample discharge tube 32 and arranging it in one-to-one correspondence with each reagent kit 31 can further optimize the liquid transfer path and improve the liquid transfer efficiency.

[0033] To facilitate the transfer of the waste liquid discharge tube 34 and the finished product discharge tube 33, holding balls 35 are respectively provided at both ends of the top surface of the waste liquid discharge tube 34 and / or the finished product discharge tube 33. When it is necessary to remove the aforementioned waste liquid discharge tube 34 and the finished product discharge tube 33, the operator can hold the holding balls 35 at both ends, which is very convenient.

[0034] Those skilled in the art can easily understand that, on the premise of no conflict, the above-mentioned advantageous ways can be freely combined and superimposed.

[0035] The above are only the preferred embodiments of the present utility model, and are not intended to limit the present utility model. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model. The above is only the preferred implementation mode of the present utility model. It should be noted that for those of ordinary skill in the art of this technology, several improvements and modifications can be made without departing from the technical principle of the present utility model, and these improvements and modifications should also be regarded as within the protection scope of the present utility model.

Claims

1. An integrated device for nucleic acid extraction and nucleic acid methylation, characterized in that, The invention comprises a bottom plate (1) and a magnetic adsorption mechanism (2), wherein the magnetic adsorption mechanism (2) comprises a magnetic attraction component (21) for adsorbing magnetic beads in a reaction tube and a driving component for driving the magnetic attraction component (21) to rise and fall, wherein the driving component comprises a screw (221) threadedly connected to the magnetic attraction component (21) and a rotary drive motor (222), wherein an output shaft (2221) of the rotary drive motor (222) and a driving end of the screw (221) are connected by a synchronous belt (223), wherein the rotation axis of the screw (221) and the rotation axis of the output shaft (2221) are arranged parallel to and spaced apart from each other, wherein the screw (221) and the rotary drive motor (222) are projected onto a vertical plane in a direction perpendicular to the output shaft (2221), and the axial length of the rotary drive motor (222) is within the axial length range of the screw (221).

2. The integrated device according to claim 1, wherein The free end face of the output shaft (2221) and the driving end face of the screw rod (221) are on the same horizontal plane.

3. The integrated device according to claim 2, characterized in that, A driving wheel (2222) is mounted on the free end of the output shaft (2221), a driven wheel (224) is mounted on the driving end of the screw rod (221), and the synchronous belt (223) is tensioned on the driving wheel (2222) and the driven wheel (224).

4. The integrated device according to claim 1, characterized in that, The invention also includes a reaction platform (3) for placing a reagent box (31), a sample tube (32) and a finished product tube (33); the reaction platform (3) is located in the top area of the base plate (1) and supported on the base plate (1); the rotary drive motor (222) is assembled on the top surface of the base plate (1) and is located in the gap space formed between the reagent box (31) and the base plate (1).

5. The integrated device according to claim 4, characterized in that, The magnetic adsorption mechanism (2) further includes a guide assembly, wherein the guide assembly includes two vertical light rods (231), the two vertical light rods (231) are slidably connected to the magnetic adsorption assembly (21) via sliding bearings (232), the bottom ends of the vertical light rods (231) are fixedly connected to the bottom plate (1), and the top ends of the vertical light rods (231) are fixedly connected to the reaction platform (3).

6. The integrated device according to claim 5, wherein The magnetic attraction component (21) includes an assembly plate (211) and a plurality of magnets (212) assembled on one side of the assembly plate (211), wherein the magnets (212) are evenly spaced along the length extension direction of the assembly plate (211) and are arranged one-to-one corresponding to the positions of the reaction tubes of the reagent kit (31), the sliding bearing (232) is connected to the assembly plate (211) as a whole, and the screw rod (221) is threadedly connected to the assembly plate (211).

7. The integrated device according to claim 4, characterized in that, A plurality of spaced and parallel first accommodating grooves are formed on the reaction platform (3), the first accommodating grooves extend in a first direction, and each of the reagent kits (31) is respectively accommodated in each of the first accommodating grooves. A second accommodating groove for accommodating the sample row pipe (32) and a third accommodating groove for accommodating the finished product row pipe (33) are further formed on the reaction platform (3). The second accommodating groove and the third accommodating groove both extend in a second direction, the second direction is perpendicular to the first direction, and the number of tube holes on the sample row pipe (32) and the finished product row pipe (33) is equal to the number of the first accommodating grooves.

8. The integrated device according to claim 7, wherein A fourth accommodating groove for accommodating the waste liquid row pipe (34) is further formed on the reaction platform (3) between the second accommodating groove and the third accommodating groove. The fourth accommodating groove extends in the second direction, and the number of tube holes on the waste liquid row pipe (34) is equal to the number of the first accommodating grooves.

9. The integrated device according to claim 8, characterized in that, Handheld balls (35) are respectively arranged at both ends of the top surface of the waste liquid row pipe (34) and / or the finished product row pipe (33).

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