Culture container suitable for suspension balling growth of cells

Abstract

The utility model belongs to the technical field of cell culture containers, and particularly discloses a culture container suitable for suspension balling growth of cells. The culture container comprises a pore plate base, a pore plate plug-in inserted into the pore plate base and a cover plate covering the pore plate plug-in; the pore plate base is provided with a plurality of first joint parts, and the pore plate plug-in is provided with a second joint part corresponding to each first joint part; the first joint part is a vertically-through hole column, the second joint part is a groove, and the first joint part and the second joint part are in butt joint to form a unit hole which is provided with an opening in the top and is used for cell culture. The cell spheroidizing device can improve the uniformity of cell growth spheroidizing, and the cell spheroidizing efficiency is high.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture containers, and particularly relates to a culture container suitable for cells growing in suspension and forming spheres. Background Art

[0002] Cell culture refers to a method of simulating the in-vivo environment (sterile, appropriate temperature, pH value, certain nutrient conditions, etc.) in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions. The process of cell culture is inseparable from the culture medium that provides nutrients and the culture container that bears the culture system.

[0003] Different types of cells have different requirements for culture containers. Currently, the more common culture containers on the market are mainly divided into TC-treated adherent cell culture containers, untreated suspension cell culture containers, and ultra-low adsorption-treated suspension cell culture containers.

[0004] Neural stem cells are a type of cells that grow in suspension and form spheres, and have a certain adsorption force. Conventionally, ultra-low adsorption culture containers are used, or an untreated culture container combined with a shaker is used for culture. However, the existing ultra-low adsorption containers are prone to forming cell spheres with uneven sizes during the culture process, and may cause over-digestion of some neural spheres and insufficient digestion of some neural spheres during the digestion process with digestive juice, thereby affecting the quality of harvested single cells. Summary of the Utility Model

[0005] To solve the above problems, the utility model provides a culture container suitable for cells growing in suspension and forming spheres, which can improve the uniformity of cell growth into spheres and has a high collection efficiency of cell spheres.

[0006] To achieve the above purpose, the specific technical solution adopted by the utility model is as follows:

[0007] A culture container suitable for cells growing in suspension and forming spheres, comprising:

[0008] A well plate base provided with a plurality of first engaging parts;

[0009] A well plate insert inserted into the well plate base, and each of the first engaging parts is provided with a second engaging part corresponding thereto. The first engaging part and the second engaging part are docked to form a unit hole with an open top for cell culture;

[0010] A cover plate covering the well plate insert.

[0011] The utility model physically isolates cells through the unit holes, realizes separated culture and spatial limitation, and effectively improves the uniformity of the formed cell spheres.

[0012] Further, the first joint part is a hole column penetrating up and down, and the second joint part is a groove; when the hole plate plug is inserted into the hole plate base, the bottom edge of the hole column fits and joins with the opening edge of the groove, so that the formed unit holes will not leak liquid. The combined design of the hole column and the groove realizes the cultivation of cells in the unit holes on the one hand, and is convenient for cell collection on the other hand. Just lift the hole column to separate it from the groove, and the culture solution can be collected together, completing the collection at one time, which is simple and efficient.

[0013] Further, the groove surface of the groove is an arc surface, that is, there are no sharp corners in the groove, which is beneficial to cell growth and collection.

[0014] Further, the grooves are evenly distributed and closely joined to improve the utilization rate of the culture container and facilitate the rapid collection of the culture solution after the culture is completed.

[0015] Further, the hole plate base includes a bottom plate and a frame provided on the upper surface of the bottom plate. The groove is provided on the upper surface of the bottom plate, and the frame encloses the groove; the hole plate plug includes a top plate, and the hole column penetrates through the top plate; the top plate is placed on the frame and is connected to the bottom plate through a buckling structure. The fixation of the hole plate plug is realized through the setting of the buckling structure, ensuring the stability of the joint between the hole column and the groove.

[0016] Further, a limiting groove for placing the top of the frame is provided on the lower surface of the top plate, which is convenient for the quick and stable installation of the hole plate plug.

[0017] Further, at least two buckling structures are symmetrically provided. Each buckling structure includes a clamping groove provided on the lower surface of the top plate and a clamping buckle provided on the upper surface of the bottom plate and buckling with the clamping groove.

[0018] Further, a pressing strip is connected to the edge of the clamping buckle. In the state where the clamping buckle is buckled with the clamping groove, the pressing strip extends out of the clamping groove. By pressing it, the clamping state of the clamping buckle is changed, and the clamping buckle is separated from the clamping groove, realizing the disassembly of the hole plate plug from the hole plate base.

[0019] The utility model has the following beneficial effects:

[0020] The utility model adopts a porous physical isolation method. Through shaking table culture, relatively uniform neurospheres are formed in independent small holes without the need for ultra-low adsorption treatment, effectively improving the quality of single cells harvested in the subsequent digestion process. In addition, it is rather cumbersome to collect neurospheres from multiple holes separately. The utility model sets the hole plate as a structure in which the base and the plug are combined to form a culture unit hole, so that when collecting cells, only by removing the plug, the multiple holes can be converted into one hole, greatly simplifying the collection operation and improving the collection efficiency. Description of the Drawings

[0021] Figure 1 : Schematic structural diagram of the culture container applicable to suspension spheroid-growing cells described in Example 1.

[0022] Figure 2 : Schematic structural diagram of the unit hole (composed of a hole column and a groove) in Example 1.

[0023] Figure 3 : Schematic diagram of the snap-fit structure between the hole plate insert and the hole plate base in Example 1.

[0024] Figure 4 : Schematic structural diagram of the pressing strip in Example 1.

[0025] In the figure: 1-hole plate base, 2-hole plate insert, 3-cover plate; 11-bottom plate, 12-frame, 13-groove, 14-snap, 15-pressing strip, 21-top plate, 22-hole column, 23-card slot. Detailed implementation manners

[0026] The following further describes the present utility model in conjunction with the accompanying drawings and specific embodiments.

[0027] Example 1

[0028] A culture container applicable to suspension spheroid-growing cells, as Figure 1 shown, includes a hole plate base 1, a hole plate insert 2 inserted into the hole plate base 1, and a cover plate 3 covering the hole plate insert 2.

[0029] The hole plate base 1 includes a bottom plate 11 and a frame 12 provided on the upper surface of the bottom plate 11; the upper surface of the bottom plate 11 is evenly distributed with closely connected grooves 13, and the groove surface of the grooves 13 is an arc surface; the frame 12 encloses all the grooves 13.

[0030] The hole plate insert 2 includes a top plate 21, and a hole column 22 is provided on the top plate 21 corresponding to each of the grooves 13; the hole column 22 penetrates through the top plate 21 and is a cylindrical structure that is vertically through; when the hole plate insert 2 is installed on the hole plate base 1, the bottom edge of the hole column 22 fits and connects with the opening edge of the groove 13 (as Figure 2 shown), forming a unit hole with an open top for cell culture.

[0031] The orifice plate insert 2 is fixedly installed on the orifice plate base 1 through the arrangement of the limiting groove and the buckling structure. Specifically, the limiting groove is formed on the lower surface of the top plate 21 for the top of the frame 12 to be placed therein; the buckling structure is located outside the limiting groove and is symmetrically provided with two left and right ones. Each buckling structure includes a clamping groove 23 formed on the lower surface of the top plate 21 and a clamping buckle 14 provided on the upper surface of the bottom plate 11 and buckled with the clamping groove 23 (as Figure 3 shown). Align the limiting groove with the frame 12 and the clamping groove 23 with the clamping buckle 14, and press down the orifice plate insert 2 to complete its installation on the orifice plate base 1.

[0032] A pressing strip 15 is connected to the outer edge of the clamping buckle 14 (as Figure 4 shown). In the state where the clamping buckle 14 is buckled with the clamping groove 23, the pressing strip 15 extends out of the clamping groove 23. By pressing it, the clamping buckle 14 is deformed to change its clamping state, and the orifice plate insert 2 can be lifted to separate the clamping buckle 14 from the clamping groove 23, so as to remove the orifice plate insert 2 from the orifice plate base 1.

[0033] The usage method of the culture container applicable to the suspension and spheroid formation of growing cells is as follows: In the initial state, the orifice plate insert 2 and the orifice plate base 1 are in the installed state. The cell suspension can be inoculated into the unit holes through a multi-channel pipette. The addition and replacement of the culture medium can both be completed through a multi-channel pipette, and then cover the cover plate 3 for cell culture. When harvesting the cells, by pressing the pressing strip 15 inward, the buckling structure of the orifice plate base 1 and the orifice plate insert 2 is opened, the orifice plate insert 2 is removed, and then the collection of cell spheres is completed at one time. Using the culture container of this embodiment to culture neural stem cells according to this method, it is observed under a microscope that the cell spheres are uniform.

[0034] This specific implementation manner is only an explanation of the present invention and does not limit the present invention. Any changes made by those skilled in the art after reading the description of the present invention, as long as they are within the scope of the claims of the present invention, will be protected by the patent law.

Claims

1. A culture container suitable for culturing cells that grow in suspension and form spheres, characterized in that: Comprising: A orifice plate base (1) provided with a plurality of first engaging portions; An orifice plate insert (2) inserted into the orifice plate base (1), and a second engaging portion is provided corresponding to each of the first engaging portions, and the first engaging portion and the second engaging portion are butted to form a unit hole with an open top for cell culture; A cover plate (3) covering the orifice plate insert (2).

2. The culture container applicable to suspension spheroid-growing cells according to claim 1, wherein: The first engaging portion is a groove (13), and the second engaging portion is a hole column (22) penetrating up and down; when the orifice plate insert (2) is inserted into the orifice plate base (1), the bottom edge of the hole column (22) fits and abuts against the opening edge of the groove (13).

3. The culture container applicable to suspension spheroid-growing cells according to claim 2, characterized in that: The groove surface of the groove (13) is an arc surface.

4. The culture container applicable to suspension spheroid-growing cells according to claim 2, wherein: The grooves (13) are evenly distributed and closely adjoin each other.

5. The culture container applicable to suspension spheroid-growing cells according to claim 2, characterized in that: The orifice plate base (1) includes a bottom plate (11) and a frame (12) provided on the upper surface of the bottom plate (11), the groove (13) is provided on the upper surface of the bottom plate (11), and the frame (12) encloses the groove (13); the orifice plate insert (2) includes a top plate (21), and the hole column (22) penetrates through the top plate (21); the top plate (21) is placed on the frame (12) and is connected to the bottom plate (11) through a snap connection structure.

6. The culture container suitable for suspension spheroid growth cells according to claim 5, characterized in that: A limiting groove for placing the top of the frame (12) is provided on the lower surface of the top plate (21).

7. The culture container applicable to suspension spheroid growth cells according to claim 5, characterized in that: At least two snap connection structures are symmetrically provided, and each snap connection structure includes a card slot (23) provided on the lower surface of the top plate (21) and a snap (14) provided on the upper surface of the bottom plate (11) and engaged with the card slot (23).

8. The culture container applicable to the suspension and spheroid growth of cells according to claim 7, wherein: A pressing strip (15) is connected to the edge of the snap (14). In the state where the snap (14) is engaged with the card slot (23), the pressing strip (15) extends out of the card slot (23), and by pressing it, the clamping state of the snap (14) is changed, and the snap (14) is separated from the card slot (23).

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