Multifunctional operation table for rapid nucleic acid detection
By designing the pressure gradient and filter filtration technology of the multi-functional operating table, the problem of large area and slow construction of traditional PCR laboratories is solved, and fast and safe nucleic acid testing is achieved, which is suitable for the emergency needs of fever clinics.
Patent Information
- Application Number
- CN202422140753.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Priority Date
- 2024-08-01
- Filing Date
- 2024-09-02
- Publication Date
- 2025-07-29
- Estimated Expiration
- 2034-09-02
AI Technical Summary
Traditional PCR laboratories cover a large area, have high site requirements and slow construction speed, making it difficult to quickly layout in the early stages of infectious disease outbreaks, and are immovable, resulting in waste of resources, and the nucleic acid testing time is long, which affects the emergency treatment of fever patients.
A multi-functional operating table is designed, including a reagent preparation area, a specimen preparation area and an amplification analysis area. It is equipped with a clean workbench, a biosafety cabinet and a fume hood, which adopts pressure gradient design and filter filtration to achieve the distinction between positive and negative pressure, and forms a clean environment through the supply and exhaust fans, which is integrated into a compact modular equipment.
It has achieved fast, safe and low-cost nucleic acid testing, which can be completed by one person within 2 hours. It is suitable for a small number of samples in the emergency department of the fever clinic and multiple frequency tests. It complies with PCR laboratory standards, covers a small area and is fast in construction, and reduces resource waste.
Smart Images
Figure CN223164316U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of laboratory equipment, in particular to a multifunctional operation table for rapid nucleic acid detection. Background Art
[0002] Nucleic acid detection plays an important role in controlling and preventing major infectious disease epidemics. At present, most PCR laboratories are located in major hospitals. Traditional PCR laboratories cover a large area, have high requirements for the site, and slow construction speed. In the initial stage of the outbreak of infectious diseases, it is difficult to quickly complete the layout construction, and the golden period of epidemic prevention and control may be missed. Moreover, due to the immobility of PCR laboratories, a large number of PCR laboratories may cause huge waste after the epidemic.
[0003] Gene amplification experiment, also known as PCR experiment, is a detection method specifically used to detect viral infectious diseases such as AIDS and hepatitis B. It can measure whether specific viruses are contained in the bodies of some infected people with low virus content by amplifying the genes contained in the virus. Since this detection method can detect viruses that are difficult to detect by ordinary tests and has the advantages of high sensitivity, high specificity, fast speed, and low requirements for samples, it is widely recognized by clinicians and has been widely used in clinical diagnosis in hospitals and infectious disease diagnosis in various epidemic prevention and detection departments. However, this experiment requires a laboratory that can ensure absolute safety, reasonable configuration, and very standardized operation as a prerequisite. Therefore, the PCR experiment needs to be carried out in a PCR laboratory. Traditional PCR laboratories cover a large area, have high requirements for the site, and slow construction speed. In the initial stage of the outbreak of infectious diseases, it is difficult to quickly complete the layout construction, and the golden period of epidemic prevention and control may be missed. Moreover, due to the immobility of PCR laboratories, a large number of PCR laboratories may cause huge waste after the epidemic. Content of the Utility Model
[0004] In view of the deficiencies of the prior art, the utility model provides a multifunctional operation table for rapid nucleic acid detection, which has a fast start, high efficiency, safety and reliability, and convenient operation.
[0005] The present utility model is realized through the following technical solutions. There is provided a multi-functional operation table for rapid nucleic acid detection, which includes three operation rooms arranged side by side. The three operation rooms are respectively a reagent preparation area, a specimen preparation area, and an amplification analysis area. A clean workbench, a biological safety cabinet, and a fume hood are respectively provided in the reagent preparation area, the specimen preparation area, and the amplification analysis area. Transfer windows are installed between the reagent preparation area and the specimen preparation area, between the specimen preparation area and the amplification analysis area, on the side of the reagent preparation area far from the specimen preparation area, and on the side of the amplification analysis area far from the specimen preparation area. The operation room includes a left side plate, a right side plate, a front plate, a rear plate, and a top plate; a tempered glass and two glove ports are installed on the front plate. A supply air fan is installed on the top of the reagent preparation area, and exhaust air fans are installed on the tops of the specimen preparation area and the amplification analysis area.
[0006] In this solution, personnel can operate and transfer the reagents in the clean workbench, biological safety cabinet, and fume hood through the glove ports. The clean workbench in the reagent preparation area is used for reagent preparation work, the biological safety cabinet in the specimen preparation area is used for specimen preparation work, and sample analysis work is carried out in the fume hood in the amplification analysis area. All materials are transferred through the transfer windows without interference. The reagent preparation area is maintained at positive pressure by the supply air fan, and the specimen preparation area and the amplification analysis area are maintained at negative pressure by the exhaust air fans.
[0007] As an optimization, primary filters are installed at the air inlets of the supply air fan and the exhaust air fans, and high-efficiency filters are installed at the air outlets of the supply air fan and the exhaust air fans. The primary filters and high-efficiency filters in this solution have a good filtering effect.
[0008] As an optimization, a door is installed on the rear plate, and a door lock is installed on the door. By setting the door, personnel can enter and exit from the rear, which is convenient for equipment maintenance and disinfection and cleaning operations before and after use.
[0009] As an optimization, a lighting lamp and an ultraviolet lamp are installed on the top plate. The lighting lamp in this solution plays a lighting role, and the ultraviolet lamp plays a disinfection role.
[0010] As an optimization, the transfer window includes a transfer housing and transfer doors installed at both ends of the transfer housing. Transfer door handles are installed on the transfer doors. The two transfer doors in this solution will not be opened simultaneously, thus avoiding the transmission of viruses between adjacent spaces.
[0011] As an optimization, an interlock mechanism is installed between the transfer doors at both ends of the transfer housing. The interlock mechanism in this solution prevents the simultaneous opening of the two transfer doors.
[0012] As an optimization, the clean bench, biological safety cabinet, and fume hood all include a housing, which is detachably and fixedly connected to the base. The front panel of the housing is hinged, and an internal lighting lamp and an internal ultraviolet lamp are installed inside the housing. In this solution, the housings of the clean bench, biological safety cabinet, and fume hood respectively provide three operation spaces. The opening and closing of the operation space are realized by the front panel switch. The internal lighting lamp is used for lighting the operation space, and the internal ultraviolet lamp is used for disinfecting the operation space.
[0013] As an optimization, a workbench fan that blows air inward is installed inside the housing of the clean bench. A primary workbench filter is installed at the air inlet of the workbench fan, and a high-efficiency workbench filter is installed at the air outlet of the workbench fan. The workbench fan in this solution realizes positive pressure inside the clean bench to prevent the internal operation space from being contaminated.
[0014] As an optimization, a safety cabinet fan is installed inside the housing of the clean bench. The air outlet of the safety cabinet fan is connected to the inside of the housing through a lower high-efficiency filter, and the air outlet of the safety cabinet fan is connected to the outside of the housing through an upper high-efficiency filter. In this solution, the safety cabinet fan and the lower high-efficiency filter provide a Class 100 clean environment for the operation area.
[0015] As an optimization, a fume hood fan that blows air outward is installed inside the housing of the fume hood. The fume hood fan in this solution is used to provide negative pressure.
[0016] The beneficial effects of the present utility model are as follows:
[0017] 1. It solves the problems that traditional PCR laboratories require huge investments in human, material, and financial resources, with serious waste of various resources, and the nucleic acid detection time is relatively long, ranging from 4 or 5 hours to 1 day at the shortest. In terms of timeliness, it seriously delays the treatment of fever patients and emergency patients.
[0018] 2. Adopting the "one-step nucleic acid extraction" solution, one person can quickly perform nucleic acid detection, and the nucleic acid detection results can be issued within 2 hours. The fever clinic and emergency department can quickly and low-costly carry out the next step of treatment. The overall solution is more suitable for nucleic acid detection of a small number of samples and multiple frequencies in the fever clinic and emergency department.
[0019] 3. It is small in size and complete in function. The three areas are closely designed and do not interfere with each other, ensuring the safety of each detection link, reducing the sample transfer time between areas, and improving the efficiency of rapid nucleic acid detection.
[0020] 4. There is a pressure gradient between the three areas. The reagent preparation area is a positive pressure area with a pressure of 5 - 10 pa. The specimen preparation area is a negative pressure area with a pressure of -10 - -15 pa. The amplification and analysis area is also a negative pressure area with a pressure of -15 - -20 pa, ensuring the accuracy and safety of each detection link and meeting the construction standards of PCR laboratories.
[0021] 5. Large floor area, small site requirements, and fast construction speed. It can quickly complete the layout construction at the initial stage of the outbreak of infectious diseases. Description of the Drawings
[0022] Figure 1 Front internal view of the present utility model;
[0023] Figure 2 Back structure schematic diagram of the present utility model;
[0024] Figure 3 Front internal view of the back of the present utility model;
[0025] Figure 4 Front structure schematic diagram of the present utility model;
[0026] Figure 5 Side internal view of the clean bench of the present utility model;
[0027] Figure 6 Structure schematic diagram of the clean bench of the present utility model;
[0028] Figure 7 Side internal view of the biosafety cabinet of the present utility model;
[0029] Figure 8 Side internal view of the fume hood of the present utility model;
[0030] Figure 9 Front internal view of the transfer window of the present utility model;
[0031] Figure 10 Side view of the transfer window of the present utility model;
[0032] Figure 11 Front view of the transfer window of the present utility model;
[0033] Figure 12 Structure schematic diagram of the transfer window of the present utility model;
[0034] As shown in the figure:
[0035] 1. Reagent Preparation Area, 2. Specimen Preparation Area, 3. Amplification and Analysis Area, 4. Clean Bench, 5. Biological Safety Cabinet, 6. Fume Hood, 7. Transfer Window, 8. Left Panel, 9. Right Panel, 10. Front Panel, 11. Rear Panel, 12. Top Panel, 13. Tempered Glass, 14. Glove Ports, 15. Control Panel, 16. Supply Air Fan, 17. Lighting Lamp, 18. Ultraviolet Lamp, 19. Door, 20. Door Lock, 21. Differential Pressure Gauge, 22. Hinge, 23. Visual Window, 24. Louver, 26. High Efficiency Filter, 27. Primary Filter, 28. Air Inlet, 29. Exhaust Air Fan, 30. Housing, 31. Base, 32. Primary Filter for Workbench, 33. Workbench Fan, 34. High Efficiency Filter for Workbench, 35. Internal Lighting Lamp, 36. Internal Ultraviolet Lamp, 37. Control Panel Panel, 38. Front Panel, 39. Hinge, 40. Caster, 41. Upper High Efficiency Filter, 42. Lower High Efficiency Filter, 43. Safety Cabinet Fan, 44. Fume Hood Fan, 45. Transfer Housing, 46. Sealing Strip, 47. Transfer Door, 48. Transfer Door Handle, 49. Transfer Window Ultraviolet Lamp, 50. Interlock Mechanism. Detailed Implementation Manner
[0036] To clearly illustrate the technical features of this solution, the following will elaborate on this solution through specific implementation manners.
[0037] As Figures 1 to 12 shown, a multifunctional operating table for rapid nucleic acid detection of the present utility model includes three juxtaposed operating rooms. The three operating rooms are respectively a Reagent Preparation Area 1, a Specimen Preparation Area 2, and an Amplification and Analysis Area 3. A Clean Bench 4, a Biological Safety Cabinet 5, and a Fume Hood 6 are respectively provided in the Reagent Preparation Area 1, the Specimen Preparation Area 2, and the Amplification and Analysis Area 3.
[0038] The operating room includes a Left Panel 8, a Right Panel 9, a Front Panel 10, a Rear Panel 11, and a Top Panel 12, thus splicing into a rectangular space; two Tempered Glass 13 and two Glove Ports 14 are installed on the Front Panel 10, facilitating the operation of the user.
[0039] A Lighting Lamp 17 and an Ultraviolet Lamp 18 are installed on the Top Panel 12. The Lighting Lamp 17 can make the brightness reach the required range, and the Ultraviolet Lamp 18 can disinfect the environment. A Door 19 is installed on the Rear Panel 11, and a Door Lock 20 is installed on the Door 19. It is convenient for maintenance personnel to enter and maintain the equipment. The door is fixedly installed on the Rear Panel 11 through a Hinge 22. A Visual Window 23 is installed on the Door 19, and the situation inside can be observed. Louvers 24 are installed on both the Rear Panel 11 and the Door 19, which can achieve a certain amount of gas flow. This flow rate should not be too large, so as to facilitate the realization of positive and negative pressure. A Differential Pressure Gauge 21 is installed on the Rear Panel 11, and the differential pressure inside can be observed.
[0040] The rear panel is equipped with a control panel 15, which can control the switches of the lighting lamp 17, the ultraviolet lamp 18, and each blower.
[0041] The reagent preparation area 1 should maintain a certain positive pressure. Therefore, a supply blower 16 is installed at the top of the reagent preparation area 1. The air inlet of the supply blower 16 is equipped with a primary filter 27, and the air outlet of the supply blower 16 is equipped with a high-efficiency filter 26, generating a certain, uniform, and highly clean cross-sectional air velocity, exhausting the original air in the working area, blowing away dust particles and biological particles, so as to form a sterile and highly clean working environment.
[0042] Exhaust blowers 29 are installed at the tops of both the specimen preparation area 2 and the amplification and analysis area 3. Thus, the airflows in the specimen preparation area 2 and the amplification and analysis area 3 are exhausted to ensure a negative pressure environment in the specimen preparation area 2 and the amplification and analysis area 3. The air inlet of the exhaust blower 29 is equipped with a primary filter 27, and the air outlet of the exhaust blower 29 is equipped with a high-efficiency filter 26. The airflow flowing into the operation area is for personnel protection, the downward airflow filtered by the high-efficiency filter is for product protection, and the airflow inside the equipment is discharged after being filtered by the high-efficiency filter for environmental protection.
[0043] Transfer windows 7 are installed between the reagent preparation area 1 and the specimen preparation area 2, between the specimen preparation area 2 and the amplification and analysis area 3, on the side of the reagent preparation area 1 far from the specimen preparation area 2, and on the side of the amplification and analysis area 3 far from the specimen preparation area 2. Specifically, the left side plate 8 of the reagent preparation area 1, the clean workbench 4, and the transfer window 7 are connected and fixed by bolts, and sealing treatment is done at the connection. The right side plate 9 of the reagent preparation area 1, the clean workbench 4, the left side plate 8 of the specimen preparation area, the biological safety cabinet 5, and the transfer window 7 are connected and fixed by bolts, and sealing treatment is done at the splicing. The right side plate 9 of the specimen preparation area 2, the biological safety cabinet 5, the left side plate 8 of the amplification and analysis area, the fume hood 6, and the transfer window 7 are connected and fixed by bolts, and sealing treatment is done at the splicing. The fume hood 6, the right side plate 9 of the amplification and analysis area, and the transfer window 7 are connected and fixed by bolts, and sealing treatment is done at the splicing.
[0044] As Figures 9 - 12 shown, the transfer window 7 includes a transfer housing 45 and transfer doors 47 installed at both ends of the transfer housing 45. A transfer door handle 48 is installed on the transfer door 47. A sealing strip 46 is installed between the transfer door 47 and the transfer housing 45 to ensure sealing after the transfer door 47 is closed. A transfer window ultraviolet lamp 49 is installed inside the transfer housing 45, which can disinfect and sterilize the interior.
[0045] An interlock mechanism 50 is installed between the transfer doors 47 at both ends of the transfer housing 45. It can ensure that the two transfer doors 47 cannot be opened simultaneously, preventing cross-contamination of airflows. The interlock mechanism 50 adopts an existing structure, and this application does not limit the interlock mechanism.
[0046] The clean bench 4, biological safety cabinet 5 and fume hood 6 have similar structures. All three include a housing 30, and the three housings 30 are respectively arranged in three operation rooms. The housing 30 is detachably and fixedly connected to the base 31. The housing 30 and the base 31 are of a split structure and are connected and fixed by bolts. Casters 40 are installed on the base, and the cabinet can be moved.
[0047] The housing 30 is equipped with an internal lighting lamp 35 and an internal ultraviolet lamp 36. A front panel 38 is hinged to the front of the housing 30. The front panel 38 is hinged to the top of the housing through a hinge 39. A control panel 37 is installed on the front panel 38. The internal lighting lamp 35 can be controlled to turn on and off through the control panel 37 to ensure the illuminance requirement inside the housing 30. The internal ultraviolet lamp 36 can be controlled through the control panel 37 to disinfect and sterilize the environment in the operation area after the test.
[0048] The housing 30 of the clean bench 4 is equipped with a bench blower 33 that blows air inward. A bench primary filter 32 is installed at the air inlet of the bench blower 33, and a bench high-efficiency filter 34 is installed at the air outlet of the bench blower 33. After the air flow is filtered through the bench primary filter 32 and the bench high-efficiency filter 34, a hundred-class clean environment can be achieved in the operation area.
[0049] The housing 30 of the clean bench 4 is equipped with a safety cabinet blower 43. The air outlet of the safety cabinet blower 43 is communicated with the inside of the housing 30 through a lower high-efficiency filter 42, and the air outlet of the safety cabinet blower 43 is communicated with the outside of the housing 30 through an upper high-efficiency filter 41. The safety cabinet blower 43 can be controlled to turn on and off and adjust the speed through the control panel 37, and the air flow can be sucked in through the front window.
[0050] The upper high-efficiency filter 41 is installed at the top of the housing, which can purify the discharged gas and protect the external environment. A part of the air flow is discharged after being purified by the upper high-efficiency filter 41, and a part of the air flow is purified by the lower high-efficiency filter 42 to provide a hundred-class clean environment for the operation area.
[0051] The housing 30 of the fume hood 6 is equipped with a fume hood blower 44 that blows air outward. The fume hood blower 44 can be controlled to turn on and off and adjust the speed through the control panel 37. After the air flow is purified by the high-efficiency filter, the external environment can be protected.
[0052] The usage method of the present utility model:
[0053] Before the start of the experiment, turn on the control panels in the reagent preparation area, specimen preparation area, and amplification analysis area. Press the fan key and the lighting key. By adjusting the wind speed, make the pressure in the reagent preparation area exceed the outdoor pressure by 5 - 10 Pa, the pressure in the specimen preparation area reach 10 - (-5) Pa lower than the outdoor pressure, and the pressure in the amplification analysis area reach 15 - 20 Pa lower than the outdoor pressure. The pressure value can be checked through a differential pressure gauge. After the pressure meets the requirements, turn on the fan and lighting keys of the clean bench, biological safety cabinet, and fume hood through the front window glove ports in the reagent preparation area, standard preparation area, and amplification analysis area, and purify the operation area for about 15 minutes. After the purification is completed, take out the reagents for the experiment, transfer them to the operation area of the clean bench through the transfer window, and carry out the reagent preparation work. After the reagent preparation is completed, transfer them to the operation area of the biological safety cabinet through the transfer window to carry out the specimen preparation work. After the specimen preparation is completed, transfer them to the amplification analysis area through the transfer window to carry out the sample analysis work. After the sample analysis is completed, upload the data. Take out the used samples through the transfer window and throw them into the trash can to complete the entire nucleic acid detection process. Then, through the front window operation port, turn off the fan and lighting of the clean bench, biological safety cabinet, and fume hood, close the front window glass, turn on the ultraviolet light to disinfect the environment in the operation area, and turn on the ultraviolet lamps in the reagent preparation area, specimen preparation area, and amplification analysis area to disinfect the environment inside.
[0054] Of course, the above description is not limited to the above examples. The technical features not described in the present utility model can be achieved by or adopted from the prior art, and will not be elaborated here. The above embodiments and drawings are only used to illustrate the technical solutions of the present utility model and are not intended to limit the present utility model. The present utility model has been described in detail with reference to the preferred embodiments. Those of ordinary skill in the art should understand that any changes, modifications, additions, or substitutions made by those of ordinary skill in the art within the scope of the essence of the present utility model do not depart from the purpose of the present utility model and should also fall within the protection scope of the claims of the present utility model.
Claims
1. A multifunctional operating table for rapid nucleic acid detection, characterized in that: It includes three side-by-side operation rooms. Inside the three operation rooms are a reagent preparation area (1), a specimen preparation area (2), and an amplification and analysis area (3) respectively. Inside the reagent preparation area (1), the specimen preparation area (2), and the amplification and analysis area (3) are respectively equipped with a clean bench (4), a biological safety cabinet (5), and a fume hood (6). Between the reagent preparation area (1) and the specimen preparation area (2), between the specimen preparation area (2) and the amplification and analysis area (3), on the side of the reagent preparation area (1) far from the specimen preparation area (2), and on the side of the amplification and analysis area (3) far from the specimen preparation area (2), transfer windows (7) are installed. The operation room includes a left side plate (8), a right side plate (9), a front plate (10), a rear plate (11), and a top plate (12); On the front plate (10), tempered glass (13) and two glove ports (14) are installed. On the top of the reagent preparation area (1), a supply air fan (16) is installed. On the tops of the specimen preparation area (2) and the amplification and analysis area (3), exhaust air fans (29) are installed.
2. The multifunctional operation table for rapid nucleic acid detection according to claim 1, wherein: Primary filters (27) are installed at the air inlets of the supply air fan (16) and the exhaust air fans (29), and high-efficiency filters (26) are installed at the air outlets of the supply air fan (16) and the exhaust air fans (29).
3. The multifunctional operating table for rapid nucleic acid detection according to claim 1, characterized in that: A door (19) is installed on the rear plate (11), and a door lock (20) is installed on the door (19).
4. A multifunctional operating table for rapid nucleic acid detection according to claim 1, characterized in that: A lighting lamp (17) and an ultraviolet lamp (18) are installed on the top plate (12).
5. The multifunctional operating table for rapid nucleic acid detection according to claim 1, wherein: The transfer window (7) includes a transfer housing (45) and transfer doors (47) installed at both ends of the transfer housing (45). Transfer door handles (48) are installed on the transfer doors (47).
6. The multifunctional operating table for rapid nucleic acid detection according to claim 5, characterized in that: An interlock mechanism (50) is installed between the transfer doors (47) at both ends of the transfer housing (45).
7. The multifunctional operation table for rapid nucleic acid detection according to claim 1, wherein: The clean bench (4), the biological safety cabinet (5), and the fume hood (6) all include a housing (30). The housing (30) is detachably fixed to a base (31). A front panel (38) is hinged to the front of the housing (30). Inside the housing (30), an internal lighting lamp (35) and an internal ultraviolet lamp (36) are installed.
8. A multifunctional operating table for rapid nucleic acid detection according to claim 7, characterized in that: Inside the housing (30) of the clean bench (4), a bench fan (33) that blows air inward is installed. A bench primary filter (32) is installed at the air inlet of the bench fan (33), and a bench high-efficiency filter (34) is installed at the air outlet of the bench fan (33).
9. The multifunctional operation table for rapid nucleic acid detection according to claim 7, wherein: Inside the housing (30) of the clean bench (4), a safety cabinet fan (43) is installed. The air outlet of the safety cabinet fan (43) is connected to the inside of the housing (30) through a lower high-efficiency filter (42), and the air outlet of the safety cabinet fan (43) is connected to the outside of the housing (30) through an upper high-efficiency filter (41).
10. A multifunctional operating table for rapid nucleic acid detection according to claim 7, characterized in that: Inside the housing (30) of the fume hood (6), a fume hood fan (44) that blows air outward is installed.