Microcarrier liquid changing device

Abstract

The utility model relates to a microcarrier liquid changing device, and belongs to the technical field of microcarriers, the microcarrier liquid changing device comprises a culture medium bottle and a microcarrier bottle, a first liquid outlet pipe is inserted into the culture medium bottle, a first liquid inlet pipe is inserted into the microcarrier bottle, and a connecting assembly for connecting the first liquid outlet pipe and the first liquid inlet pipe is arranged at the upper end of the first liquid outlet pipe and the upper end of the first liquid inlet pipe; a pressure assembly for driving a culture medium to enter the first liquid outlet pipe is mounted in the culture medium bottle, and a liquid discharging assembly for driving waste liquid in the microcarrier bottle to be discharged is arranged on the microcarrier bottle. The method has the effect of reducing the contamination risk caused by liquid change.

Description

Technical Field

[0001] The present application relates to the technical field of microcarriers, and particularly relates to a microcarrier liquid changing device. Background Art

[0002] A microcarrier is a microbead used for adherent cell growth, generally composed of natural dextran or various synthetic polymers. When in use, the microcarriers are added to the culture medium in a culture container, and the microcarriers are kept in a suspended state by continuous agitation. After adding cells to the culture container, the cells will adhere to the surface of the microcarriers and grow and reproduce.

[0003] Before the microcarriers are added to the culture container, they need to be sterilized at high temperature and soaked with a sterile culture medium multiple times. Currently, the main method is to manually pour the culture medium into the microcarrier bottle after opening the lid, which is likely to cause bacteria to enter the microcarrier bottle through the bottle mouth, resulting in a high risk of contamination. Utility Model Content

[0004] In order to reduce the risk of contamination caused by liquid change, the present application provides a microcarrier liquid changing device.

[0005] The microcarrier liquid changing device provided by the present application adopts the following technical solutions:

[0006] A microcarrier liquid changing device includes a culture medium bottle and a microcarrier bottle. A first liquid outlet pipe is inserted into the culture medium bottle, and a first liquid inlet pipe is inserted into the microcarrier bottle. A connection component for connecting the two is provided at the upper ends of the first liquid outlet pipe and the first liquid inlet pipe. A pressure component for driving the culture medium in the culture medium bottle into the first liquid outlet pipe is installed in the culture medium bottle, and a liquid discharging component for driving the waste liquid in the microcarrier bottle to be discharged is provided on the microcarrier bottle.

[0007] By adopting the above technical solutions, after the microcarriers in the microcarrier bottle are precipitated, the supernatant in the microcarrier bottle is discharged through the liquid discharging component. At this time, the pressure component drives the culture medium in the culture medium bottle into the first liquid outlet pipe. Then, the culture medium in the first liquid outlet pipe enters the microcarrier bottle through the connection component and the first liquid inlet pipe. After shaking the microcarrier bottle and placing it, when the microcarriers are precipitated, the supernatant is discharged again through the liquid discharging component, so as to facilitate the liquid change of the microcarrier bottle without moving the culture medium bottle, reduce the possibility of bacteria entering the culture medium bottle, and further reduce the risk of contamination caused by liquid change.

[0008] Optionally, the pressure component includes a first peristaltic pump.

[0009] By adopting the above technical solutions, the first peristaltic pump applies positive pressure to the culture medium bottle, thereby pressing the culture medium in the culture medium bottle into the first liquid outlet pipe and transporting it to the microcarrier bottle through the connection component and the first liquid inlet pipe, reducing the possibility of manual pouring and the risk of contamination caused by liquid change.

[0010] Optionally, the liquid discharge assembly includes a waste liquid bucket located on the side of the microcarrier bottle away from the culture medium bottle. A second liquid outlet pipe is inserted into the microcarrier bottle. The microcarriers and the clear liquid in the microcarrier bottle are stratified. The lower end of the second liquid outlet pipe is located on the side of the clear liquid close to the microcarriers. A second liquid inlet pipe is inserted into the waste liquid bucket. The upper ends of the second liquid inlet pipe and the second liquid outlet pipe are also provided with a connection assembly connecting the two, and a negative pressure assembly for pumping the waste liquid in the microcarrier bottle into the second liquid inlet pipe is provided on the second liquid inlet pipe.

[0011] By adopting the above technical solution, the negative pressure assembly pumps the clear liquid in the microcarrier bottle into the waste liquid bucket through the second liquid inlet pipe, the connection assembly and the second liquid outlet pipe, thereby reducing the possibility of the microcarriers being poured out with the clear liquid due to manual pouring of the microcarrier bottle, and at the same time reducing the risk of bacterial contamination caused by liquid replacement.

[0012] Optionally, the negative pressure assembly includes a second peristaltic pump installed on the second liquid inlet pipe.

[0013] By adopting the above technical solution, the second peristaltic pump applies negative pressure to the second liquid inlet pipe. At this time, the clear liquid in the microcarrier bottle enters the second liquid outlet pipe under the action of pressure and moves to the waste liquid bucket through the connection assembly and the second liquid inlet pipe, thereby reducing the possibility of the microcarriers being discharged with the clear liquid during the process of manually moving and pouring the microcarrier bottle, and at the same time reducing the risk of bacterial contamination caused by liquid replacement.

[0014] Optionally, openings are provided at the upper ends of the culture medium bottle, the microcarrier bottle and the waste liquid bucket. Lids for blocking the openings are provided at the upper ends of the culture medium bottle, the microcarrier bottle and the waste liquid bucket. The first liquid inlet pipe, the first liquid outlet pipe, the second liquid inlet pipe and the second liquid outlet pipe all include insertion parts located under the corresponding lids, and connection parts located above the lids are provided on the upper sides of the insertion parts. Communication assemblies connecting the insertion parts and the connection parts are provided on the lids.

[0015] By adopting the above technical solution, the first liquid inlet pipe, the first liquid outlet pipe, the second liquid inlet pipe and the second liquid outlet pipe are all fixed on the corresponding lids through the communication assemblies, so as to facilitate controlling the position of the lower ends of the insertion parts, facilitating liquid replacement of the microcarrier bottle, reducing the possibility of manually pouring the culture medium bottle and the microcarrier bottle, and further reducing the risk of bacterial contamination caused by liquid replacement.

[0016] Optionally, the connecting component includes a first fixing portion located on the upper side of the cover body. The lower ends of the connecting portions are all sleeved on the upper ends of the first fixing portion. A fixing member for fixing the connecting portions is provided on the first fixing portion. A second fixing portion is provided on the lower side of the cover body. The inserting portions are all sleeved on the lower ends of the second fixing portion. A fixing member for fixing the inserting portions is also provided on the second fixing portion. The lower end of the first fixing portion passes through the corresponding cover body, and the upper end of the second fixing portion is sleeved on the lower end of the first fixing portion and is threadedly connected to the first fixing portion.

[0017] By adopting the above technical solution, the connecting portions and the inserting portions are fixed on the corresponding cover bodies by means of threaded connection of the first fixing portion and the second fixing portion, which improves the installation efficiency. At the same time, it is convenient to control the position of the lower end of the inserting portion. And when the connecting portions and the inserting portions need to be replaced, it is convenient to disassemble and replace the new connecting portions and inserting portions, improving the replacement efficiency.

[0018] Optionally, the fixing member includes a cable tie sleeved on the outer side of the lower end of the connecting portion. The cable tie is in interference fit with the upper end of the first fixing portion. The inserting portion is also fixed to the second fixing portion by the cable tie.

[0019] By adopting the above technical solution, the cable tie fixes the connecting portion on the first fixing portion, and the second fixing portion is also fixed to the inserting portion by the cable tie, so as to facilitate the installation of the connecting portion and the inserting portion on the cover body, improving the installation efficiency.

[0020] Optionally, two sealing rings are sleeved on the lower end of the first fixing portion. When the lower end of the first fixing portion passes through the corresponding cover body and is fixed to the second fixing portion, the sealing rings are located on both sides of the cover body, and the mutually approaching sides of the sealing rings are in contact with the side wall of the cover body.

[0021] By adopting the above technical solution, when the first fixing portion and the second fixing portion fix the connecting portion and the inserting portion on the corresponding cover body, the sealing rings are located on both sides of the cover body and are in contact with the cover body, reducing the possibility of the culture medium detaching from the culture medium bottle through the gap between the first fixing portion and the cover body, and at the same time reducing the possibility of the microcarrier detaching from the microcarrier bottle through the gap between the first fixing portion and the cover body. At the same time, it reduces the possibility of bacteria entering the culture medium bottle and the microcarrier bottle, reducing the risk of bacterial contamination caused by liquid change.

[0022] In summary, the present application includes at least one of the following beneficial technical effects:

[0023] 1. When the microcarrier precipitation in the microcarrier bottle is completed, the supernatant in the microcarrier bottle is discharged through the liquid discharge assembly. At this time, the culture medium in the culture medium bottle is driven by the pressure assembly into the first liquid outlet pipe. Then, the culture medium in the first liquid outlet pipe enters the microcarrier bottle through the connection assembly and the first liquid inlet pipe. After shaking the microcarrier bottle and placing it, when the microcarrier precipitates, the supernatant is discharged again through the liquid discharge assembly, so as to facilitate the liquid change of the microcarrier bottle without moving the culture medium bottle, reduce the possibility of bacteria entering the culture medium bottle, and further reduce the risk of bacterial contamination caused by liquid change;

[0024] 2. The negative pressure assembly extracts the supernatant in the microcarrier bottle to the waste liquid bucket through the second liquid inlet pipe, the connection assembly and the second liquid outlet pipe, thus reducing the possibility of the microcarrier being poured out with the supernatant due to manual pouring of the microcarrier bottle, and at the same time reducing the risk of bacterial contamination caused by liquid change;

[0025] 3. The first liquid inlet pipe, the first liquid outlet pipe, the second liquid inlet pipe and the second liquid outlet pipe are all fixed on the corresponding cover body through the connection assembly, so as to facilitate the control of the position of the lower end of the insertion part, facilitate the liquid change of the microcarrier bottle, reduce the possibility of manual pouring of the culture medium bottle and the microcarrier bottle, and further reduce the risk of bacterial contamination caused by liquid change. Description of the Drawings

[0026] Figure 1 is the overall structural schematic diagram of the microcarrier liquid change device in the embodiment of the present application.

[0027] Figure 2 is the structural schematic diagram showing the positional relationship between the liquid discharge assembly and the microcarrier bottle in the embodiment of the present application.

[0028] Figure 3 is Figure 2 the enlarged view of the structure at A in

[0029] Description of the reference numerals: 1, culture medium bottle; 11, opening; 12, cover body; 13, first liquid outlet pipe; 131, insertion part; 132, connection part; 2, microcarrier bottle; 21, first liquid inlet pipe; 3, quick connector; 4, liquid discharge assembly; 41, waste liquid bucket; 42, second liquid outlet pipe; 43, second liquid inlet pipe; 5, connection assembly; 51, first fixing part; 52, tie strap; 53, second fixing part; 54, sealing ring. Detailed Embodiments

[0030] The following further describes the present application in detail with reference to the drawings.

[0031] The embodiment of the present application discloses a microcarrier liquid change device. Refer to Figure 1 and Figure 2, a microcarrier liquid exchange device includes a culture medium bottle 1 for holding culture medium and a microcarrier bottle 2 for holding microcarriers. Both the upper ends of the culture medium bottle 1 and the microcarrier bottle 2 are provided with openings 11, and the upper ends of the culture medium bottle 1 and the microcarrier bottle 2 are covered with a cover body 12 for blocking the openings 11. A first liquid outlet pipe 13 is inserted into the culture medium bottle 1, the lower end of the first liquid outlet pipe 13 is close to the bottom wall inside the culture medium bottle 1, and the upper end of the first liquid outlet pipe 13 passes through the corresponding cover body 12. A first liquid inlet pipe 21 is inserted into the microcarrier bottle 2, the upper end of the first liquid inlet pipe 21 also passes through the corresponding cover body 12, and a connection component for connecting the two is provided at the upper ends of the first liquid outlet pipe 13 and the second liquid inlet pipe 43.

[0032] Referring to Figure 1 and Figure 2 , in the embodiment of the present application, the connection component is selected as a Swagelok brand quick connector 3 with the model B-QC8-D1-810. A pressure component for pressing the culture medium into the first liquid outlet pipe 13 is provided in the culture medium bottle 1. In the embodiment of the present application, the pressure component is set as a first peristaltic pump (not shown in the figure). In other embodiments, the pressure component can be an air inlet pipe for introducing compressed air into the culture medium bottle 1 or a vacuum pressure pump. A liquid drainage component 4 for discharging the clear liquid in the microcarrier bottle 2 is provided on one side of the microcarrier bottle 2 away from the culture medium bottle 1.

[0033] After the microcarriers in the microcarrier bottle 2 are precipitated, the clear liquid in the microcarrier bottle 2 is discharged through the liquid drainage component 4. At this time, the first peristaltic pump applies positive pressure to the culture medium bottle 1, so as to press the culture medium in the culture medium bottle 1 into the first liquid outlet pipe and transport it to the microcarrier bottle 2 through the quick connector 3 and the first liquid inlet pipe 21. After the microcarrier bottle 2 is shaken and placed, when the microcarriers are precipitated, the clear liquid is discharged again through the liquid drainage component 4 to complete the liquid exchange.

[0034] Referring to Figure 1 and Figure 2 , the liquid drainage component 4 includes a waste liquid bucket 41 on one side of the microcarrier bottle 2 away from the culture medium bottle 1. The upper end of the waste liquid bucket 41 is also provided with an opening 11, and the upper end of the waste liquid bucket 41 is also covered with a cover body 12 for blocking the opening 11. A second liquid outlet pipe 42 is inserted into the side of the microcarrier bottle 2 close to the waste liquid bucket 41, and a second liquid inlet pipe 43 is inserted into the waste liquid bucket 41. The microcarriers and the clear liquid in the microcarrier bottle 2 are stratified, and the second liquid outlet pipe 42 is located on the side of the clear liquid close to the microcarriers and does not contact the microcarriers.

[0035] The upper ends of the second liquid outlet pipe 42 and the second liquid inlet pipe 43 both pass through the corresponding cover body 12, and a connection component for connecting the two is also provided at the upper ends of the second liquid outlet pipe 42 and the second liquid inlet pipe 43. In the embodiment of the present application, the connection component is a quick connector 3 of the Swagelok brand with the model B-QC8-D1-810. A negative pressure component for driving the clear liquid in the microcarrier bottle 2 to enter the waste liquid bucket 41 is provided on the second liquid inlet pipe 43. In the embodiment of the present application, the negative pressure component includes a second peristaltic pump (not shown in the figure) installed on the second liquid inlet pipe 43.

[0036] The second peristaltic pump applies negative pressure to the second liquid inlet pipe 43. At this time, the clear liquid in the microcarrier bottle 2 enters the second liquid outlet pipe 42 under the action of pressure and moves to the waste liquid bucket 41 through the quick connector 3 and the second liquid inlet pipe 43, reducing the possibility of the microcarrier being discharged with the clear liquid during the process of manually moving the microcarrier bottle 2 and pouring, and at the same time reducing the risk of bacterial contamination caused by liquid change.

[0037] Refer to Figure 2 and Figure 3 As shown in, the first liquid inlet pipe 21, the first liquid outlet pipe 13, the second liquid inlet pipe 43 and the second liquid outlet pipe 42 all include vertical insertion parts 131 located on the lower side of the corresponding cover body 12. Connection parts 132 corresponding to the insertion parts 131 and located on the upper side of the corresponding cover body 12 are provided at the upper ends of the insertion parts 131. A communication component 5 for connecting the insertion part 131 and the corresponding connection part 132 is provided on each cover body 12.

[0038] Refer to Figure 2 and Figure 3 As shown in, the communication component 5 includes a first fixing part 51 corresponding to the connection part 132 and located on the upper side of the cover body 12. The upper ends of the connection parts 132 are all sleeved on the upper ends of the first fixing part 51, and a fixing piece for fixedly connecting the connection part 132 is provided on the first fixing part 51. In the embodiment of the present application, the fixing piece is set as a cable tie 52 sleeved on the outer side of the lower end of the connection part 132, and the connection part 132 is fixed to the corresponding first fixing part 51 through the cable tie 52.

[0039] Second fixing parts 53 corresponding to the first fixing part 51 are provided on the lower sides of the cover bodies 12. The upper ends of the insertion parts 131 are all sleeved on the outer sides of the lower ends of the second fixing parts 53, and a fixing piece for fixing the insertion part 131 is provided on the second fixing part 53. In the embodiment of the present application, the fixing piece is set as a cable tie 52 sleeved on the outer side of the upper end of the insertion part 131, and the insertion part 131 is fixed to the corresponding second fixing part 53 through the cable tie 52.

[0040] The diameter of the middle part of the first fixing part 51 is larger than that of the lower end of the first fixing part 51, and the diameter of the upper end of the second fixing part 53 is also larger than that of the lower end of the second fixing part 53. The lower end of the first fixing part 51 passes through the corresponding cover body 12, and the upper end of the second fixing part 53 is sleeved and threadedly connected to the outside of the end of the first fixing part 51 passing through the cover body 12. Two horizontal sealing rings 54 are sleeved on the lower end of the first fixing part 51. When the first fixing part 51 is fixed to the corresponding second fixing part 53, the sealing rings 54 are symmetrically arranged on both sides of the corresponding cover body 12 and are in contact with the side wall of the cover body 12.

[0041] The cable tie 52 fixes the connecting part 132 to the first fixing part 51, and the second fixing part 53 is also fixed to the inserting part 131 by the cable tie 52. The connecting part 132 and the inserting part 131 are fixed to the corresponding cover body 12 by the threaded connection of the first fixing part 51 and the second fixing part 53, which improves the installation efficiency. At the same time, it is convenient to control the position of the lower end of the inserting part 131. And when the connecting part 132 and the inserting part 131 need to be replaced, it is convenient to disassemble and replace the new connecting part 132 and inserting part 131, which improves the replacement efficiency.

[0042] When the first fixing part 51 and the second fixing part 53 fix the connecting part 132 and the inserting part 131 to the corresponding cover body 12, the sealing rings 54 are located on both sides of the cover body 12 and are in contact with the cover body 12, reducing the possibility of the culture medium detaching from the culture medium bottle 1 through the gap between the first fixing part 51 and the cover body 12, reducing the possibility of the microcarrier detaching from the microcarrier bottle 2 through the gap between the first fixing part 51 and the cover body 12, and at the same time reducing the possibility of bacteria entering the culture medium bottle 1 and the microcarrier bottle 2.

[0043] The implementation principle of a microcarrier liquid change device in an embodiment of the present application is as follows: After the microcarriers in the microcarrier bottle 2 are precipitated, the supernatant in the microcarrier bottle 2 is discharged into the waste liquid bucket 41. The first peristaltic pump applies positive pressure to the culture medium bottle 1, presses the culture medium in the culture medium bottle 1 into the first liquid outlet pipe 13 and transports it to the microcarrier bottle 2 through the quick connector 3 and the first liquid inlet pipe 21. After the microcarrier bottle 2 is shaken evenly and placed, when the microcarriers are precipitated, the supernatant is discharged again to complete the liquid change.

[0044] The above are all the preferred embodiments of the present application, and the protection scope of the present application is not limited thereby. Therefore, all equivalent changes made according to the structure, shape, and principle of the present application should be covered within the protection scope of the present application.

Claims

1. A microcarrier liquid change device, characterized in that: It includes a culture medium bottle (1) and a microcarrier bottle (2). A first liquid outlet pipe (13) is inserted into the culture medium bottle (1), and a first liquid inlet pipe (21) is inserted into the microcarrier bottle (2). A connecting component for connecting the two is provided at the upper ends of the first liquid outlet pipe (13) and the first liquid inlet pipe (21). A pressure component for driving the culture medium into the first liquid outlet pipe (13) is installed in the culture medium bottle (1). A liquid discharging component (4) for driving the waste liquid in the microcarrier bottle (2) to be discharged is provided on the microcarrier bottle (2).

2. The microcarrier liquid change device according to claim 1, characterized in that: The pressure component includes a first peristaltic pump.

3. The microcarrier liquid change device according to claim 2, characterized in that: The liquid discharging component (4) includes a waste liquid bucket (41) located on the side of the microcarrier bottle (2) away from the culture medium bottle (1). A second liquid outlet pipe (42) is inserted into the microcarrier bottle (2). The microcarriers and the clear liquid in the microcarrier bottle (2) are stratified. The lower end of the second liquid outlet pipe (42) is located on the side of the clear liquid close to the microcarriers. A second liquid inlet pipe (43) is inserted into the waste liquid bucket (41). A connecting component for connecting the two is also provided at the upper ends of the second liquid inlet pipe (43) and the second liquid outlet pipe (42). And a negative pressure component for pumping the waste liquid in the microcarrier bottle (2) into the second liquid inlet pipe (43) is provided on the second liquid inlet pipe (43).

4. The microcarrier liquid changing device according to claim 3, characterized in that: The negative pressure component includes a second peristaltic pump installed on the second liquid inlet pipe (43).

5. The microcarrier liquid change device according to claim 4, characterized in that: Openings (11) are provided at the upper ends of the culture medium bottle (1), the microcarrier bottle (2), and the waste liquid bucket (41). Covers (12) for blocking the openings (11) are provided at the upper ends of the culture medium bottle (1), the microcarrier bottle (2), and the waste liquid bucket (41). The first liquid inlet pipe (21), the first liquid outlet pipe (13), the second liquid inlet pipe (43), and the second liquid outlet pipe (42) each include an insertion part (131) located below the corresponding cover (12). A connecting part (132) located above the cover (12) is provided above the insertion part (131). A communicating component (5) for connecting the insertion part (131) and the connecting part (132) is provided on each cover (12).

6. The microcarrier liquid change device according to claim 5, characterized in that: The communicating component (5) includes a first fixing part (51) located above the cover (12). The lower ends of the connecting parts (132) are sleeved on the upper ends of the first fixing parts (51). Fixing pieces for fixing the connecting parts (132) are provided on the first fixing parts (51). A second fixing part (53) is provided below the cover (12). The insertion parts (131) are sleeved on the lower ends of the second fixing parts (53). Fixing pieces for fixing the insertion parts (131) are also provided on the second fixing parts (53). The lower end of the first fixing part (51) passes through the corresponding cover (12), and the upper end of the second fixing part (53) is sleeved on the lower end of the first fixing part (51) and is threadedly connected to the first fixing part (51).

7. The microcarrier liquid changing device according to claim 6, characterized in that: The fixing member includes a cable tie (52) sleeved on the outer side of the lower end of the connecting portion (132). The cable tie (52) is in interference fit with the upper end of the first fixing portion (51). The insertion portion (131) is also fixed to the second fixing portion (53) through the cable tie (52).

8. The microcarrier liquid change device according to claim 7, wherein: Two sealing rings (54) are sleeved on the lower end of the first fixing portion (51). When the lower end of the first fixing portion (51) passes through the corresponding cover body (12) and is fixed to the second fixing portion (53), the sealing rings (54) are located on both sides of the cover body (12), and the side of the sealing rings (54) close to each other abuts against the side wall of the cover body (12).

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