Liquid strain culture device

Abstract

The utility model discloses a liquid strain culture device which comprises a main body unit, the main body unit comprises an activation tank and a domestication tank, the activation tank is provided with a microbial agent storage cylinder, the bottom of the microbial agent storage cylinder is fixed and communicated with a first guide pipe, the end, away from the microbial agent storage cylinder, of the first guide pipe is fixed and communicated with the activation tank, and the first guide pipe is provided with a first electromagnetic valve; nutrient substance storage cylinders are fixed to the outer sides of the tops of the activation tank and the domestication tank, second guide pipes are fixed to and communicated with the bottoms of the nutrient substance storage cylinders, and the ends, away from the nutrient substance storage cylinders, of the second guide pipes are fixed to and communicated with the corresponding activation tank and domestication tank. Through the arrangement of the activation tank and the domestication tank, the activation tank is used for preliminarily culturing and activating a microbial inoculum, and when a bacterial liquid obtained after preliminary culture in the activation tank is discharged into the domestication tank, the domestication tank is used for further expanding culture and domestication of a strain, so that the strain adapts to a specific wastewater environment; and the strains adapting to a specific wastewater environment can be domesticated to a greater extent through the multi-time culture.

Description

Technical Field

[0001] The utility model relates to the technical field of strain culture, in particular to a liquid strain culture device. Background Art

[0002] In the field of biological treatment, strain expansion culture devices are widely used in the culture and amplification of microbial agents. The main function of these devices is to provide a suitable growth environment for microorganisms, so as to increase the number and activity of strains by controlling factors such as nutrient supply and gas exchange.

[0003] The existing liquid strain culture devices usually inject wastewater into a tank body and then carry out the culture of strains. During the culture process of the strains, they gradually increase and strengthen, and they will consume the wastewater in the tank body, resulting in that the strains after culture are not better domesticated and cannot better adapt to a specific wastewater environment. Moreover, when injecting wastewater into the liquid strain culture device, the wastewater needs to be disinfected first. However, after long-term use of the disinfectant, it is not convenient to replace it. In addition, when the existing liquid strain culture device cultures strains, it is also necessary to supply oxygen to the device. Oxygen can fully promote the metabolism and growth of strains. However, when supplying oxygen, after the oxygen supply pipe discharges oxygen, large bubbles will form and rise in the device. The large bubbles cause most of the oxygen in the bubbles not to be provided to the strains, resulting in low oxygen utilization rate. Summary of the Utility Model

[0004] The purpose of this part is to outline some aspects of the embodiments of the utility model and briefly introduce some preferred embodiments. Simplifications or omissions may be made in this part as well as in the abstract of the specification and the title of the utility model of this application to avoid obscuring the purpose of this part, the abstract of the specification and the title of the utility model. Such simplifications or omissions shall not be used to limit the scope of the utility model.

[0005] To solve the above technical problems, the utility model provides the following technical solution: A liquid strain culture device, comprising:

[0006] A main body unit, which includes an activation tank and a domestication tank. A bacterial agent storage cylinder is arranged on the activation tank. The bottom of the bacterial agent storage cylinder is fixedly connected and communicated with a first conduit. And the end of the first conduit away from the bacterial agent storage cylinder is fixedly connected and communicated with the activation tank. A first solenoid valve is installed on the first conduit. Nutrient storage cylinders are fixedly arranged on the outer sides of the tops of the activation tank and the domestication tank. The bottom of the nutrient storage cylinder is fixedly connected and communicated with a second conduit. And the end of the second conduit away from the nutrient storage cylinder is fixedly connected and communicated with the corresponding activation tank and domestication tank. And a second solenoid valve is installed on the second conduit. Exhaust pipes are fixedly connected and communicated with the tops of the activation tank and the domestication tank.

[0007] The feeding unit includes a wastewater conduit, and both ends of the wastewater conduit are fixedly connected and communicated with the tops of the activation tank and the domestication tank respectively. A third conduit is fixedly connected and communicated in the middle of the wastewater conduit. One end of the third conduit away from the wastewater conduit is fixedly connected and communicated with a disinfection cylinder. A water inlet pipe is fixedly connected to the bottom of the disinfection cylinder. A disinfection component for disinfecting wastewater is arranged in the disinfection cylinder. An aeration component is also arranged between the activation tank and the domestication tank.

[0008] As a preferred scheme of the liquid strain culture device of the present utility model, wherein: a drain pipe is fixedly connected and communicated with the bottom of the activation tank, and a solenoid valve three is installed on the drain pipe. The bottom of the drain pipe is fixedly connected and communicated with the top of the domestication tank. A plurality of brackets are fixed to the bottom of the activation tank, and the bottoms of the brackets are fixed to the top of the domestication tank.

[0009] As a preferred scheme of the liquid strain culture device of the present utility model, wherein: a solenoid valve five for controlling the injection of wastewater into the activation tank is installed on the wastewater conduit, and a solenoid valve four for controlling the injection of wastewater into the domestication tank is also installed on the third conduit.

[0010] As a preferred scheme of the liquid strain culture device of the present utility model, wherein: the disinfection component includes a cylinder body that can be embedded into the disinfection cylinder. A sealing cover is threadedly connected to the bottom of the cylinder body. A plurality of small holes are opened in the cylinder body and the sealing cover, and a disinfectant is filled in the cylinder body and the sealing cover. The disinfectant is located between the third conduit and the water inlet pipe.

[0011] As a preferred scheme of the liquid strain culture device of the present utility model, wherein: a connecting shaft is fixed to the top of the cylinder body, and a nut is fixed to the top of the connecting shaft. The nut is threadedly connected to the disinfection cylinder.

[0012] As a preferred scheme of the liquid strain culture device of the present utility model, wherein: the aeration component includes an oxygen conduit. Both ends of the oxygen conduit are fixedly connected to the bottoms of the activation tank and the domestication tank respectively and extend into the activation tank and the domestication tank. A plurality of arc-shaped pipes are fixed to the outer sides of the oxygen conduit extending into the activation tank and the domestication tank, and a plurality of air holes are opened in the plurality of arc-shaped pipes.

[0013] As a preferred scheme of the liquid strain culture device of the present utility model, wherein: an air inlet pipe is fixedly connected and communicated with the middle of the oxygen conduit, and a sterile filter is installed in the air inlet pipe. A solenoid valve seven for controlling the injection of oxygen into the activation tank is installed on the oxygen conduit, and a solenoid valve six for controlling the injection of oxygen into the domestication tank is also installed on the oxygen conduit.

[0014] The beneficial effects of the present utility model:

[0015] 1. Through the settings of the activation tank and the domestication tank, the activation tank is used for the preliminary cultivation and activation of the bacterial agent. When the bacterial liquid after preliminary cultivation in the activation tank is discharged into the domestication tank, the domestication tank at this time is used for further culturing and domestication of the bacterial species to make it adapt to a specific wastewater environment. The step-by-step cultivation can domesticate bacterial species that are more adaptable to a specific wastewater environment to a greater extent.

[0016] 2. Through the settings of the arc-shaped tube and the air holes, and the air holes are relatively small, making the discharged oxygen bubbles small, so that oxygen can fully promote the metabolism and growth of the bacterial species.

[0017] 3. After the disinfectant has been used for a period of time, its disinfection effect is poor and it needs to be replaced. At this time, turn the nut to separate it from the disinfection cylinder, then take out the disinfection component from the disinfection cylinder, and then turn the sealing cover to pour out the disinfectant from the cylinder body, so as to replace it with a new disinfectant. This method is very convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings. Among them:

[0019] Figure 1 It is a schematic structural diagram of a liquid bacterial species culture device of the present invention.

[0020] Figure 2 It is a schematic structural diagram of the aeration component provided by the present invention.

[0021] Figure 3 It is a schematic exploded structural diagram of the disinfection component provided by the present invention.

[0022] Figure 4 It is an enlarged partial cross-sectional structural diagram of a liquid bacterial species culture device of the present invention.

[0023] Description of the drawings: 100, main unit; 101, activation tank; 102, domestication tank; 103, microbial agent storage cylinder; 104, conduit 1; 105, solenoid valve 1; 106, nutrient storage cylinder; 107, conduit 2; 108, solenoid valve 2; 109, drain pipe; 110, solenoid valve 3; 111, bracket; 112, vent pipe; 200, feeding unit; 201, wastewater conduit; 202, conduit 3; 203, disinfection cylinder; 204, water inlet pipe; 205, disinfection component; 2051, cylinder body; 2052, sealing cover; 2053, disinfectant; 207, solenoid valve 4; 208, solenoid valve 5; 209, aeration component; 2091, oxygen conduit; 2092, arc tube; 2093, air hole; 210, solenoid valve 6; 211, solenoid valve 7; 212, air inlet pipe; 213, sterile filter; 214, nut; 215, connecting shaft. Detailed implementation manners

[0024] To make the above objects, features and advantages of the present utility model more obvious and understandable, the following detailed description of the specific implementation manners of the present utility model will be given in conjunction with the drawings of the specification.

[0025] In the following description, many specific details are set forth to fully understand the present utility model. However, the present utility model can also be implemented in other ways different from those described herein. Those skilled in the art can make similar generalizations without departing from the connotation of the present utility model. Therefore, the present utility model is not limited by the specific embodiments disclosed below.

[0026] Secondly, the so-called "one embodiment" or "embodiment" herein refers to a specific feature, structure or characteristic that can be included in at least one implementation manner of the present utility model. The "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment that excludes other embodiments.

[0027] Furthermore, the present utility model will be described in detail in conjunction with the schematic diagrams. When detailing the embodiments of the present utility model, for the convenience of explanation, the cross-sectional views showing the device structure will be enlarged locally out of the general proportion, and the schematic diagrams are only examples and should not limit the scope of protection of the present utility model herein. In addition, in actual production, three-dimensional spatial dimensions including length, width and depth should be included.

[0028] Refer to Figures 1-4 , for an embodiment of the present utility model, a liquid strain culture device is provided, which includes: a main unit 100 and a feeding unit 200;

[0029] Among them, the main body unit 100 includes an activation tank 101 and a domestication tank 102. A bacterial agent storage cylinder 103 is arranged on the activation tank 101. A first conduit 104 is fixedly connected to the bottom of the bacterial agent storage cylinder 103 and is communicated therewith. One end of the first conduit 104 away from the bacterial agent storage cylinder 103 is fixedly connected to and communicated with the activation tank 101. An electromagnetic valve one 105 is installed on the first conduit 104. Nutrient storage cylinders 106 are fixedly arranged on the outer sides of the tops of the activation tank 101 and the domestication tank 102. A second conduit 107 is fixedly connected to the bottom of the nutrient storage cylinder 106 and is communicated therewith. One end of the second conduit 107 away from the nutrient storage cylinder 106 is fixedly connected to and communicated with the corresponding activation tank 101 and domestication tank 102. An electromagnetic valve two 108 is installed on the second conduit 107. Drain pipes 112 are fixedly connected to and communicated with the tops of the activation tank 101 and the domestication tank 102;

[0030] The feeding unit 200 includes a wastewater conduit 201. The two ends of the wastewater conduit 201 are respectively fixedly connected to and communicated with the tops of the activation tank 101 and the domestication tank 102. A third conduit 202 is fixedly connected to and communicated with the middle of the wastewater conduit 201. One end of the third conduit 202 away from the wastewater conduit 201 is fixedly connected to and communicated with a disinfection cylinder 203. A water inlet pipe 204 is fixedly connected to the bottom of the disinfection cylinder 203. A disinfection assembly 205 for disinfecting wastewater is arranged in the disinfection cylinder 203. An aeration assembly 209 is further arranged between the activation tank 101 and the domestication tank 102.

[0031] In addition, a drain pipe 109 is fixedly connected to and communicated with the bottom of the activation tank 101. An electromagnetic valve three 110 is installed on the drain pipe 109. The bottom of the drain pipe 109 is fixedly connected to and communicated with the top of the domestication tank 102. A plurality of brackets 111 are fixedly arranged at the bottom of the activation tank 101. The bottoms of the brackets 111 are fixed to the top of the domestication tank 102. An electromagnetic valve five 208 for controlling the injection of wastewater into the activation tank 101 is installed on the wastewater conduit 201. An electromagnetic valve four 207 for controlling the injection of wastewater into the domestication tank 102 is also installed on the third conduit 202. The aeration assembly 209 includes an oxygen conduit 2091. The two ends of the oxygen conduit 2091 are respectively fixed to the bottoms of the activation tank 101 and the domestication tank 102 and extend into the activation tank 101 and the domestication tank 102. A plurality of arc-shaped pipes 2092 are fixed to the outer sides of the oxygen conduit 2091 extending into the activation tank 101 and the domestication tank 102. A plurality of air holes 2093 are formed in the plurality of arc-shaped pipes 2092.

[0032] It should be noted that the diameters of the plurality of arc-shaped pipes 2092 are different, so that there is a distance between the arc-shaped pipes 2092, and the sizes of the air holes 2093 are small. Then, when oxygen is input into the arc-shaped pipes 2092 from the oxygen conduit 2091 and then discharged from the air holes 2093, the discharged oxygen bubbles are small, so that the oxygen can fully promote the metabolism and growth of the bacterial strains.

[0033] When culturing liquid spawn, initially, solenoid valve four 207 is closed and solenoid valve five 208 is opened, allowing wastewater to be injected into activation tank 101 from water inlet pipe 204, disinfection cylinder 203, conduit three 202, and wastewater conduit 201. When the wastewater passes through disinfection cylinder 203, disinfection assembly 205 disinfects the wastewater. After the disinfected wastewater is injected into activation tank 101, solenoid valve one 105 is then opened, enabling the spawn agent stored in spawn agent storage cylinder 103 to be discharged into activation tank 101, ensuring the continuous supply of the spawn agent. Next, solenoid valve two 108 is opened, allowing the nutrients stored in nutrient storage cylinder 106 to be injected into activation tank 101 to provide the nutrients required for the growth of the spawn. Then, solenoid valve seven 211 is opened and solenoid valve six 210 is closed, enabling oxygen to be input into activation tank 101 from air inlet pipe 212, sterile filter 213, and oxygen conduit 2091. These devices can provide a continuous oxygen supply, promoting the metabolism and growth of the spawn. Moreover, the setting of sterile filter 213 can prevent external pollutants from entering the culture environment. The above processes are used for the preliminary cultivation and activation of the spawn agent. After preliminary cultivation in activation tank 101, solenoid valve three 110 is then opened to discharge the preliminarily cultivated spawn liquid in activation tank 101 into acclimation tank 102. At this time, acclimation tank 102 is used for further expansion culture and acclimation of the spawn to make it adapt to a specific wastewater environment. When further expanding and acclimating the preliminarily cultivated spawn liquid in acclimation tank 102, wastewater is injected into acclimation tank 102 by opening solenoid valve four 207, nutrients in nutrient storage cylinder 106 are injected into acclimation tank 102 by opening solenoid valve two 108, and oxygen is input into acclimation tank 102 by opening solenoid valve six 210, enabling the preliminarily cultivated spawn liquid to be effectively further expanded and acclimated.

[0034] In addition, disinfection assembly 205 includes a cylinder body 2051 that can be embedded into disinfection cylinder 203. A sealing cover 2052 is threadedly connected to the bottom of cylinder body 2051. Multiple small holes are provided in cylinder body 2051 and sealing cover 2052, and a disinfectant 2053 is filled in cylinder body 2051 and sealing cover 2052. The disinfectant 2053 is located between conduit three 202 and water inlet pipe 204. A connecting shaft 215 is fixed to the top of cylinder body 2051, and a nut 214 is fixed to the top of connecting shaft 215, and nut 214 is threadedly connected to disinfection cylinder 203. The middle of oxygen conduit 2091 is fixed and communicated with air inlet pipe 212, and a sterile filter 213 is installed in air inlet pipe 212. A solenoid valve seven 211 for controlling the injection of oxygen into activation tank 101 is installed on oxygen conduit 2091, and a solenoid valve six 210 for controlling the injection of oxygen into acclimation tank 102 is also installed on oxygen conduit 2091.

[0035] It should be noted that the disinfectant 2053 can be disinfectant particles containing chlorine compounds, disinfectant particles containing chlorates, ozone particles, and disinfectant particles containing chlorine compounds. For example, trichloroisocyanurate can kill various germs, bacteria, viruses and other microorganisms; disinfectant particles containing chlorates release free chlorine at different pH values to kill bacteria, viruses and other microorganisms, and can destroy the cell membranes of bacteria to kill different types of bacteria and viruses; ozone particles are suitable for disinfecting wastewater containing radioactive contamination, organic matter, etc.

[0036] During use, when the disinfectant 2053 has a poor disinfection effect after being used for a period of time and needs to be replaced, turn the nut 214 to separate it from the disinfection cylinder 203, then take out the disinfection component 205 from the disinfection cylinder 203, and then turn the sealing cover 2052 to pour out the disinfectant 2053 from the cylinder body 2051, so as to replace the new disinfectant 2053. This method is very convenient.

[0037] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present invention, and they should all be covered within the scope of the claims of the present invention.

Claims

1. A liquid spawn culture device, characterized in that, Comprising: A main body unit (100) including an activation tank (101) and a domestication tank (102). A microbial agent storage cylinder (103) is provided on the activation tank (101). A first conduit (104) is fixedly connected to the bottom of the microbial agent storage cylinder (103) and is in communication therewith. One end of the first conduit (104) far from the microbial agent storage cylinder (103) is fixedly connected to and in communication with the activation tank (101). An electromagnetic valve one (105) is installed on the first conduit (104). Nutrient storage cylinders (106) are fixedly provided on the outer sides of the tops of both the activation tank (101) and the domestication tank (102). A second conduit (107) is fixedly connected to the bottom of the nutrient storage cylinder (106) and is in communication therewith. One end of the second conduit (107) far from the nutrient storage cylinder (106) is fixedly connected to and in communication with the corresponding activation tank (101) and domestication tank (102). An electromagnetic valve two (108) is installed on the second conduit (107). Vent pipes (112) are fixedly connected to the tops of both the activation tank (101) and the domestication tank (102). A feeding unit (200) including a wastewater conduit (201). Both ends of the wastewater conduit (201) are fixedly connected to and in communication with the tops of the activation tank (101) and the domestication tank (102) respectively. A third conduit (202) is fixedly connected to the middle of the wastewater conduit (201). One end of the third conduit (202) far from the wastewater conduit (201) is fixedly connected to and in communication with a disinfection cylinder (203). A water inlet pipe (204) is fixedly connected to the bottom of the disinfection cylinder (203). A disinfection assembly (205) for disinfecting the wastewater is provided in the disinfection cylinder (203). An aeration assembly (209) is further provided between the activation tank (101) and the domestication tank (102).

2. The liquid spawn culture device according to claim 1, wherein: A drain pipe (109) is fixedly connected to the bottom of the activation tank (101) and is in communication therewith. An electromagnetic valve three (110) is installed on the drain pipe (109). The bottom of the drain pipe (109) is fixedly connected to the top of the domestication tank (102). A plurality of supports (111) are fixedly provided at the bottom of the activation tank (101). The bottoms of the supports (111) are fixed to the top of the domestication tank (102).

3. The liquid spawn culture device according to claim 1, characterized in that: An electromagnetic valve five (208) for controlling the injection of wastewater into the activation tank (101) is installed on the wastewater conduit (201). An electromagnetic valve four (207) for controlling the injection of wastewater into the domestication tank (102) is further installed on the third conduit (202).

4. A liquid spawn culture device according to claim 1, characterized in that: The disinfection assembly (205) includes a cylinder body (2051) that can be embedded into the disinfection cylinder (203). A sealing cover (2052) is threadedly connected to the bottom of the cylinder body (2051). A plurality of small holes are formed in the cylinder body (2051) and the sealing cover (2052). A disinfectant (2053) is filled in the cylinder body (2051) and the sealing cover (2052). The disinfectant (2053) is located between the third conduit (202) and the water inlet pipe (204).

5. The liquid spawn culture device according to claim 4, characterized in that: A connecting shaft (215) is fixed to the top of the cylinder body (2051), a nut (214) is fixed to the top of the connecting shaft (215), and the nut (214) is in threaded connection with the disinfection cylinder (203).

6. The liquid spawn culture device according to claim 1, wherein: The aeration assembly (209) includes an oxygen conduit (2091). Both ends of the oxygen conduit (2091) are respectively fixed to the bottoms of the activation tank (101) and the domestication tank (102) and extend into the activation tank (101) and the domestication tank (102). A plurality of arc-shaped pipes (2092) are fixed to the outer sides of the oxygen conduit (2091) extending into the activation tank (101) and the domestication tank (102), and a plurality of air holes (2093) are formed in the plurality of arc-shaped pipes (2092).

7. The liquid spawn culture device according to claim 6, wherein: An intake pipe (212) is fixed and communicated with the middle of the oxygen conduit (2091), a sterile filter (213) is installed on the intake pipe (212), an electromagnetic valve seven (211) for controlling the injection of oxygen into the activation tank (101) is installed on the oxygen conduit (2091), and an electromagnetic valve six (210) for controlling the injection of oxygen into the domestication tank (102) is also installed on the oxygen conduit (2091).

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