Mycoplasma obtaining device

Abstract

The utility model relates to and discloses a mycoplasma acquisition device which comprises an acquisition bottle, a pressure relief component, a filter and a negative pressure pipe, a rubber plug for sealing a port of the negative pressure tube is arranged on the negative pressure tube, a port of the collection bottle is connected with an inlet end of the filter, an outlet end of the filter is connected with a puncture flow guide part for puncturing the rubber plug for sealing the port of the negative pressure tube so as to communicate an outlet of the filter with the inside of the negative pressure tube, and the pressure relief part is communicated with the inside and the outside of the collection bottle. Liquid collected in the collection bottle passes through the filter and flows into the negative pressure pipe through the puncture flow guide component, impurity removal and sterilization of collected specimens can be completed when the liquid flows through the filter, and the mode does not need tedious transfer operation, is not prone to being polluted by environmental bacteria, and can effectively improve the acquisition success rate and the acquisition efficiency.

Description

Technical Field

[0001] The utility model relates to the field of mycoplasma acquisition, and more specifically, to a mycoplasma acquisition device. Background Art

[0002] Respiratory diseases have been restricting the development of the livestock breeding industry in China. In particular, respiratory diseases caused by mixed infections of multiple pathogens based on mycoplasma infection are particularly serious.

[0003] Mycoplasma is a type of the smallest prokaryotic cell microorganism without cell walls and with high pleomorphism. After mycoplasma infection, it mainly attaches to the ciliated epithelial cells of the respiratory tract, reducing ciliary activity, reducing antibacterial mucus, and causing respiratory symptoms in the body. Moreover, it increases the probability of other pathogenic microorganisms invading the body. Mycoplasma gallisepticum disease, Mycoplasma synoviae disease, and Mycoplasma hyopneumoniae in pigs, etc., which are caused by mycoplasma, are extremely prone to concurrent mixed infections with other bacteria and viruses such as Escherichia coli. Therefore, preventing and controlling mycoplasma infection is the top priority in breeding. And the collection, isolation and culture of mycoplasma, and in vitro drug tests are of great significance for guiding clinical medication. Among them, mycoplasma acquisition is the first step in the whole process.

[0004] In the work of mycoplasma acquisition, the sampling of mycoplasma samples is a crucial step in disease diagnosis. The sampling site, method, sample preservation, and transportation are all directly related to the final diagnosis result. In the operation process of traditional sampling methods, operations such as impurity removal, disinfection, and culture of swabs (throat swabs, anal swabs, etc.) need to be transferred between multiple test tubes and culture dishes. The operation is cumbersome, and it is easily contaminated by environmental bacteria. The acquisition success rate is low, and professional personnel and professional knowledge are required. Summary of the Utility Model

[0005] The utility model aims to overcome the defects of the above-mentioned prior art and provides a mycoplasma acquisition device, which is used to solve the technical problems that operations such as impurity removal, disinfection, and culture of swabs need to be transferred between multiple test tubes and culture dishes, the operation is cumbersome, and it is easily contaminated by environmental bacteria, and the acquisition success rate is low.

[0006] The technical solution adopted by the utility model is that a mycoplasma acquisition device includes a collection bottle, a pressure relief component, a filter, and a negative pressure tube; a rubber plug for sealing the port is arranged on the negative pressure tube. The port of the collection bottle is connected to the inlet end of the filter, and the outlet end of the filter is connected with a puncture and diversion component, which is used to pierce the rubber plug sealing the port of the negative pressure tube to connect the outlet of the filter with the inside of the negative pressure tube. The pressure relief component communicates the inside of the collection bottle with the outside.

[0007] First, place the swab after sampling into the mycoplasma liquid medium pre-installed in the collection bottle. Connect the port of the collection bottle to the inlet end of the filter. Use the puncture and diversion component at the outlet end of the filter to penetrate the rubber stopper. Under the action of the negative pressure in the negative pressure tube and the pressure relief component on the collection bottle, the liquid and medium collected in the collection bottle flow through the filter and into the negative pressure tube through the puncture and diversion component. When flowing through the filter, the impurity removal and bacteria removal of the collected specimen can be completed. This method does not require cumbersome transfer operations, is not easily contaminated by environmental bacteria, and can effectively improve the acquisition success rate and the acquisition efficiency.

[0008] Further, it also includes a two-way valve, and a two-way valve is provided between the port of the collection bottle and the inlet end of the filter. The flow control of the collected liquid can be achieved through the two-way valve, making the operation more convenient.

[0009] Further, the pressure relief component is a connecting pipe. The connecting pipe is fixedly arranged on the valve body of the two-way valve. One end of it leads to the bottom inside the collection bottle, and the other end is connected to the outside of the collection bottle. After the collection bottle is disassembled, the connecting pipe is separated from the collection bottle. The collection bottle is a complete bottle, and the pharyngeal swab, anal swab, etc. of animals can be collected through the collection bottle to obtain mycoplasma.

[0010] Further, the axis of the collection bottle and the axis of the negative pressure tube are parallel or coincident, and the port of the collection bottle and the port of the negative pressure tube are arranged opposite to each other. With such a setting, when filtering, it is inverted so that the collection bottle is located above and the negative pressure tube is located below. The liquid in the collection bottle can flow into the negative pressure tube through the filter, and it can also be assisted by gravity. Not only is the operation simpler, but the filtering speed can also be increased.

[0011] Further, the filter is a needle filter. The puncture and diversion component is a needle.

[0012] Further, it also includes an adapter. The two-way valve is connected to the inlet end of the filter through the adapter. The adapter has a large end and a small end. The large end of the adapter is connected to the two-way valve, and the small end of the adapter is connected to the inlet end of the filter. The adapter is mainly used to convert a larger input port into a smaller output port to adapt to the needle filter.

[0013] Further, the two-way valve and the adapter are integrally formed. Integral forming can reduce the influence of the external environment during the filtering process and ensure that the final negative pressure tube is free of bacterial and impurity contamination.

[0014] Further, there are no less than two claw catches arranged on the adapter. A snap ring is arranged on the outer peripheral surface of the port of the negative pressure tube. The claw catches are in snap-fit connection with the snap ring. When the puncture and diversion component is inserted into the negative pressure tube, the claw catches just engage with the snap ring to fix the negative pressure tube, making the pipetting more convenient.

[0015] Furthermore, when the puncture guide component is installed at the outlet end of the filter, the tip of the puncture guide component is located on the side of the claw close to the collection bottle. In this way, the length of the claw is greater than the length of the puncture guide component after installation. When touched, the claw is contacted first, and the claw can play a protective role, preventing the puncture guide component from injuring the operator.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: first, the swab after sampling is placed in the mycoplasma liquid culture medium pre-loaded in the collection bottle, the port of the collection bottle is connected to the inlet end of the filter, and the puncture guide component at the outlet end of the filter is used to penetrate the rubber stopper. Under the action of the negative pressure in the negative pressure tube and the pressure relief component on the collection bottle, the liquid collected in the collection bottle passes through the filter and the puncture guide component and flows into the negative pressure tube. When flowing through the filter, the impurity removal and sterilization of the collected specimen can be completed. This method does not require cumbersome transfer operations, is not easily contaminated by environmental bacteria, and can effectively improve the acquisition success rate and collection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0018] In the figure: 1. Collection bottle, 2. Filter, 3. Negative pressure tube, 4. Pressure relief component, 5. Rubber stopper, 6. Puncture guide component, 7. Adapter, 8. Two-way valve, 9. Claw, 10. Snap ring. DETAILED DESCRIPTION

[0019] The drawings in this utility model are for illustrative purposes only and are not to be construed as limiting the scope of this utility model. To better illustrate the following embodiments, some components in the drawings may be omitted, enlarged, or reduced in size, and do not represent the actual dimensions of the products. It is understandable to those skilled in the art that some well-known structures and their descriptions may be omitted from the drawings.

[0020] like Figure 1 As shown, the present solution discloses a mycoplasma acquisition device, comprising a collection bottle 1, a pressure relief component 4, a filter 2 and a negative pressure tube 3; the negative pressure tube 3 is provided with a rubber stopper 5 for sealing its port, the port of the collection bottle 1 is connected to the inlet end of the filter 2, and the outlet end of the filter 2 is connected to a puncture guide component 6 for piercing the rubber stopper 5 sealing the port of the negative pressure tube 3 to connect the outlet of the filter 2 with the interior of the negative pressure tube 3, and the pressure relief component 4 connects the interior and the outside of the collection bottle 1.

[0021] First, place the swab after sampling into the mycoplasma liquid medium pre-loaded in collection bottle 1 (collection bottle 1 is pre-loaded with the medium at the factory, and its port is sealed with a plastic film, which can be torn off during use). Connect the port of collection bottle 1 to the inlet end of filter 2, and use the puncture and diversion component 6 at the outlet end of filter 2 to penetrate the rubber stopper 5. Under the action of the negative pressure in negative pressure tube 3 and simultaneously under the action of the pressure relief component 4 on collection bottle 1, the liquid and medium collected in collection bottle 1 pass through filter 2 and flow into negative pressure tube 3 through the puncture and diversion component 6. When flowing through filter 2, the impurity removal and bacteria elimination of the collected specimen can be completed. This method does not require cumbersome transfer operations, is not easily contaminated by environmental bacteria, and can effectively improve the acquisition success rate and the efficiency of collection and acquisition.

[0022] It also includes a two-way valve 8, and a two-way valve 8 is arranged between the port of collection bottle 1 and the inlet end of filter 2. The flow control of the collected liquid can be realized through the two-way valve 8, making the operation more convenient.

[0023] The pressure relief component 4 is a connecting pipe, and the connecting pipe is fixedly arranged on the valve body of the two-way valve 8. One end of it leads to the inner bottom of collection bottle 1, and the other end is connected to the outside of collection bottle 1. After collection bottle 1 is disassembled, the connecting pipe is detached from collection bottle 1. Collection bottle 1 is a complete bottle, and mycoplasma can be obtained by collecting the pharyngeal swab, anal swab, etc. of animals through collection bottle 1.

[0024] The axis of collection bottle 1 and the axis of negative pressure tube 3 are parallel or coincident, and the port of collection bottle 1 and the port of negative pressure tube 3 are arranged opposite to each other. With such a setting, during filtration, it is inverted so that collection bottle 1 is located above and negative pressure tube 3 is located below. The liquid in collection bottle 1 can flow into negative pressure tube 3 through filter 2, and it can also be assisted by gravity. Not only is the operation simpler, but the gravity assistance can also increase the filtration speed.

[0025] Filter 2 is a needle filter. The puncture and diversion component 6 is a needle, and a rubber tube is sleeved on the needle before use to protect the operator from being stabbed.

[0026] It also includes an adapter 7. The two-way valve 8 is connected to the inlet end of filter 2 through the adapter 7. The adapter 7 has a large end and a small end. The large end of the adapter 7 is connected to the two-way valve 8, and the small end of the adapter 7 is connected to the inlet end of filter 2. Specifically, internal threads are provided on the inner sides of the two ports of the two-way valve 8, and external threads are provided on the port of collection bottle 1. One end of the two-way valve 8 is threadedly connected to collection bottle 1; the adapter 7 is mainly used to convert a larger input port into a smaller output port to adapt to the needle filter. Similarly, the two-way valve 8 and the adapter 7 can also be connected by threads. This split design is more convenient for replacement and maintenance. As another improved method, the two-way valve 8 and the adapter 7 can also be integrally formed. Integral forming can reduce the influence of the external environment during the filtration process and ensure that the final negative pressure tube 3 is free from bacterial and impurity contamination.

[0027] The adapter 7 is provided with no less than two clamping claws 9, and a clamping ring 10 is arranged on the outer peripheral surface of the port of the negative pressure tube 3. The clamping claws 9 are in clamping fit with the clamping ring 10. When the puncture and diversion component 6 is inserted into the negative pressure tube 3, the clamping claws 9 just engage with the clamping ring 10 to fix the negative pressure tube 3, making liquid transfer more convenient. After the puncture and diversion component 6 is installed at the outlet end of the filter 2, the tip of the puncture and diversion component 6 is located on the side of the clamping claws 9 close to the collection bottle 1. In this way, the length of the clamping claws 9 can be greater than the length of the tip of the puncture and diversion component 6 after installation. When touched, the clamping claws 9 can play a protective role and prevent the puncture and diversion component 6 from stabbing the operator.

[0028] In this solution, the negative pressure tube 3 is made of glass material, which is equivalent to a test tube and can be directly used for mycoplasma culture after impurity removal and filtration. The rest are disposable plastic materials. The filter 2 filters out impurities and bacteria. Mycoplasma is not a bacterium but a pathogenic microorganism independent of bacteria and viruses. There is a 0.45-micron filter membrane in the filter 2. The volume of bacteria is larger than the pore size of this filter membrane, so they cannot pass through the filter membrane. The volume of mycoplasma is smaller than this pore size, so it can pass through the filter membrane.

[0029] Finally, the liquid medium and mycoplasma flow into the negative pressure tube 2. The function of the filter 2 is to separate mycoplasma from other bacteria in the sample because bacteria will affect the growth and reproduction of mycoplasma.

[0030] This solution is used to collect samples from the oral and pharyngeal parts, anus, etc. of animals (common economic animals: such as chickens, ducks, pigs, poultry and livestock, as well as pets, etc.). Since the environment in the farm is very poor, traditional sampling may cause contamination if not careful. The process of filtering bacteria requires professional personnel and professional techniques. After using this solution, non-professional personnel can also operate it to prevent bacterial contamination without considering the technique.

[0031] Different from the previous use method of gradually assembling and filtering after collection, this solution can also be used as follows. When leaving the factory, the puncture and diversion part 6 has already pierced the rubber stopper 5, the two-way valve 8 is in the closed state, and the liquid medium in the collection bottle 1 has also been pre-installed and sealed. The shelf life of the medium is 6 months without problem. The assembled whole will adopt a sterilization process and independent vacuum packaging (including the sampling swab). When using, the kit is disassembled and used. The specific use process is as follows:

[0032] 1. Unpack the package; 2. Tear open the seal of the collection bottle 1 (the collection bottle 1 is sealed at the factory), and sample the animal; 3. Remove the collection bottle 1, break off the swab head of the sample and soak it in the collection bottle 1; 4. Screw the collection bottle 1 onto the two-way valve 8, gently shake it and then invert it so that the collection bottle 1 is on top and the negative pressure tube 3 is on the bottom; 5. Open the two-way valve 8, under the action of negative pressure, mycoplasma is drawn into the negative pressure tube 3, and at the same time, bacteria and impurities are removed through the filter 2; 6. Pull out the needle from the rubber stopper 5 and cover the needle cap; 7. Mail the whole negative pressure tube 3 to the laboratory; 8. Open the rubber stopper in the laboratory environment and then carry out subsequent operations. Compared with the foregoing usage method, this method is directly assembled at the factory. Thus, the operation is extremely simple and easy, no professional personnel are required, and people who do not understand professional knowledge can also operate it. Moreover, it can further reduce environmental bacterial contamination, and the success rate of obtaining mycoplasma is extremely high.

[0033] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the technical solutions of the present invention, rather than limitations on the specific implementation manners of the present invention. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the claims of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. A mycoplasma acquisition device, characterized in that: It includes a collection bottle (1), a pressure relief component (4), a filter (2) and a negative pressure tube (3); A rubber plug (5) for sealing the port is provided on the negative pressure tube (3). The port of the collection bottle (1) is connected to the inlet end of the filter (2). The outlet end of the filter (2) is connected with a puncture and diversion component (6) for puncturing the rubber plug (5) sealing the port of the negative pressure tube (3) to communicate the outlet of the filter (2) with the inside of the negative pressure tube (3). The pressure relief component (4) communicates the inside of the collection bottle (1) with the outside.

2. The mycoplasma acquisition device according to claim 1, characterized in that: It further includes a two-way valve (8), and a two-way valve (8) is provided between the port of the collection bottle (1) and the inlet end of the filter (2).

3. The mycoplasma acquisition device according to claim 2, characterized in that: The pressure relief component (4) is a connecting pipe, and the connecting pipe is fixedly arranged on the valve body of the two-way valve (8). One end of the connecting pipe leads to the inner bottom of the collection bottle (1), and the other end is connected to the outside of the collection bottle (1).

4. The mycoplasma acquisition device according to claim 3, characterized in that: The axis of the collection bottle (1) and the axis of the negative pressure tube (3) are parallel or coincident, and the port of the collection bottle (1) and the port of the negative pressure tube (3) are arranged opposite to each other.

5. The mycoplasma acquisition device according to claim 2, characterized in that: The filter (2) is a needle filter.

6. The mycoplasma acquisition device according to claim 5, characterized in that: The puncture and diversion component (6) is a needle.

7. A mycoplasma acquisition device according to claim 5, characterized in that: It further includes an adapter (7). The two-way valve (8) is connected to the inlet end of the filter (2) through the adapter (7). The adapter (7) has a large end and a small end. The large end of the adapter (7) is connected to the two-way valve (8), and the small end of the adapter (7) is connected to the inlet end of the filter (2).

8. The mycoplasma acquisition device according to claim 7, wherein: The two-way valve (8) and the adapter (7) are integrally formed.

9. The mycoplasma acquisition device according to claim 7, characterized in that: No less than two claws (9) are provided on the adapter (7). A snap ring (10) is provided on the outer peripheral surface of the port of the negative pressure tube (3). The claws (9) are in snap-fit connection with the snap ring (10).

10. A mycoplasma acquisition device according to claim 9, characterized in that: When the puncture and diversion component (6) is installed at the outlet end of the filter (2), the tip of the puncture and diversion component (6) is located on the side of the claws (9) close to the collection bottle (1).

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