Bacterial culture detection device

Abstract

The utility model discloses a bacterial culture detection device and relates to the technical field of bacterial culture. The problem of concentration regulation and control is solved. The device specifically comprises a culture chamber, bacteria are cultured in the culture chamber through a culture solution, a top cover is fixed to the outer wall of the top of the culture chamber through bolts, a concentration detection assembly is arranged on the top of the top cover, the top of the top cover is connected with a high-concentration culture solution box through a liquid supplementing pipe, and an electric control ball valve is installed on the liquid supplementing pipe. The concentration detection assembly comprises a suction pipe, a cylinder body and an electrode tip piston I, the suction pipe is fixed to the outer wall of the top of the top cover, and the bottom of the suction pipe penetrates through the top cover and then is arranged in the culture chamber. According to the utility model, by utilizing the conduction characteristics of internal and external ions of bacterial cell membranes and ions of bacterial metabolism and utilizing the electrode tip to sense the conductivity, when the number of bacteria is increased, a high-concentration nutrient solution can be automatically supplemented into the culture chamber, and malnutrition is prevented.

Description

Technical Field

[0001] The utility model relates to the technical field of bacterial culture, in particular to a bacterial culture detection device. Background Technique

[0002] Bacterial culture is a technique for growing and reproducing bacteria artificially; bacteria are widely distributed in nature, with a large quantity and a wide variety of species. They can benefit mankind or become the cause of diseases; most bacteria can be cultured artificially by inoculating them on a culture medium to make them grow and reproduce; the cultured bacteria are used for research, identification, and application.

[0003] After retrieval, the patent with the Chinese patent publication number CN113862135B discloses a bacterial culture detection device, including a bacterial culture box. A top cover is connected to the outer wall of the top of the bacterial culture box. A culture environment detection component is arranged on the inner wall of the top of the top cover, and a pump infusion component is arranged on the outer wall of the top of the top cover; the culture environment detection component includes a temperature sensor installed on the inner wall of one side of the top of the top cover, a microscopic camera installed on the inner wall of one side of the top of the top cover, and a PH sensor, a carbon dioxide sensor, and an oxygen sensor are distributed and installed on the inner wall of the other side of the top of the top cover.

[0004] The above patent has the following deficiencies: it cannot adjust the concentration of the culture solution according to the concentration of bacteria. Since the concentration of bacteria is relatively low in the early stage of culture and the total nutritional requirement is relatively low, while in the later stage of culture, the concentration of bacteria is relatively high and the nutritional requirement is relatively high. If the nutritional concentration in the early stage of culture is still maintained at this time, there will be malnutrition. Summary of the Utility Model

[0005] The purpose of the utility model is to solve the deficiencies existing in the prior art and propose a bacterial culture detection device.

[0006] In order to achieve the above purpose, the utility model adopts the following technical scheme:

[0007] A bacterial culture detection device includes a culture chamber,

[0008] Bacteria are cultured in the culture chamber with a culture solution. A top cover is fixed to the outer wall of the top of the culture chamber by bolts. A concentration detection component is arranged on the top of the top cover. A high-concentration culture solution tank is connected to the top of the top cover through a liquid supplement pipe, and an electric control ball valve is installed on the liquid supplement pipe;

[0009] The concentration detection component includes a straw, a cylinder body, and a first electrode head piston. The straw is fixed to the outer wall of the top cover, and the bottom of the straw penetrates through the top cover and is placed inside the culture chamber. The first piston is slidably connected to the inner wall of the cylinder body. Two electrode heads are fixedly embedded in the inner side wall of the cylinder body. The electrode heads are connected to a power source, and a reciprocating drive component for reciprocally driving the first piston is arranged on the top of the first piston.

[0010] Preferably: The bottom of the straw is inserted below the nutrient solution in the culture chamber and is not located at the bottom of the culture chamber.

[0011] Furthermore: The reciprocating drive component includes a support plate fixed to the top of the top cover and a motor bolted to the side wall of the support plate. The outer wall of the output shaft of the motor is successively fixed with a first crankshaft and a second crankshaft. A connecting rod one is rotatably connected to the eccentric part of the second crankshaft, and the bottom of the connecting rod one is rotatably connected to the outer wall of the top of the first piston.

[0012] Based on the foregoing solution: The electric control ball valve includes a first valve housing and a first ball core rotatably connected to the inside of the first valve housing through a first valve rod. A fixed housing is welded to the side wall of the first valve housing. A first slider is slidably connected to the inner wall of the fixed housing. A first spiral groove is formed on the outer wall of the first valve rod. A first limiting protrusion that is movably and limitedly matched with the first spiral groove is fixed to the inner wall of the first slider.

[0013] A better solution in the foregoing solution is: A permanent magnet is fixed to the side wall of the first slider, an electromagnet matched with the permanent magnet is fixed to the inner wall of the fixed housing, and a first spring is buckled on the outer wall of the other side of the first slider, and the other end of the first spring is buckled on the outer wall of the first valve housing.

[0014] As a further solution of the present utility model: The electromagnet is connected in series to the circuit of the electrode head.

[0015] Meanwhile, an automatic control ball valve is further arranged on the liquid supplement pipe. The automatic control ball valve includes a second valve housing and a second ball core rotatably connected to the inner wall of the second valve housing through a second valve rod. A second spiral groove is formed on the outer wall of the second valve rod. A second slider is slidably connected to the outer wall of the second valve rod. A second limiting protrusion that is movably and limitedly matched with the second spiral groove is fixed to the inner wall of the second slider.

[0016] As a preferred solution of the present utility model: A connecting rod two is rotatably connected to the outer wall of the second slider, and the other end of the connecting rod two is rotatably connected to the eccentric part of the first crankshaft.

[0017] Meanwhile, a fixing component is arranged outside the culture chamber. The fixing component includes a flange plate, a suction cup, and a second piston slidably connected to the inside of the suction cup. A second spring is buckled on the top of the suction cup, and the other end of the second spring is buckled on the bottom outer wall of the flange plate. I

[0018] As a more optimal solution of the present utility model: the flange is fixed to the outside of the culture chamber by bolts, the inner wall of the flange is threadedly connected with a screw rod, the top of the screw rod is fixed with a knob, and the other end of the screw rod is rotatably connected to the second piston.

[0019] The beneficial effects of the present utility model are as follows:

[0020] 1. In the present utility model, by utilizing the internal and external ions of the bacterial cell membrane and the ionic conductivity characteristics of bacterial metabolism, the conductivity is sensed by the electrode head, so that when the number of bacteria increases, a high-concentration nutrient solution can be automatically supplemented into the culture chamber to prevent malnutrition.

[0021] 2. In the present utility model, by restricting the height of the straw, a small amount of nutrient solution can be inhaled during detection, and the remaining nutrient solution in the culture chamber will not conduct electricity, thereby preventing the bacteria from being affected by the potential conditions during detection and affecting reproduction.

[0022] 3. In the present utility model, through the targeted design of the electric control ball valve, on the one hand, based on the use of electricity to measure the bacterial concentration, circuit combination is carried out to realize the automatic liquid supplement process. On the other hand, the control of the liquid supplement speed can also be realized according to the relationship between the conductivity and the bacterial concentration.

[0023] 4. In the present utility model, by setting an automatic control ball valve in series with the electric control ball valve, and the opening and closing of the automatic control ball valve are controlled by the position of the first crankshaft, so that only when the first piston is in a relatively high position, the automatic control ball valve will open to allow the nutrient solution to pass through, otherwise the electric control ball valve will not pass through even if it is opened, thereby increasing the reliability of the liquid supplement. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 FIG. 1 is a schematic diagram of the overall structure of a bacterial culture detection device proposed by the present utility model;

[0025] Figure 2 FIG. 2 is a schematic diagram of the concentration detection component structure of a bacterial culture detection device proposed by the present utility model;

[0026] Figure 3 FIG. 3 is a schematic diagram of the straw structure of a bacterial culture detection device proposed by the present utility model;

[0027] Figure 4 FIG. 4 is a schematic diagram of the circuit structure of a bacterial culture detection device proposed by the present utility model;

[0028] Figure 5 FIG. 5 is a schematic diagram of the electric control ball valve structure of a bacterial culture detection device proposed by the present utility model;

[0029] Figure 6Schematic structural diagram of a reciprocating drive assembly of a bacterial culture detection device proposed by the present utility model;

[0030] Figure 7 Schematic structural diagram of an automatic control ball valve of a bacterial culture detection device proposed by the present utility model;

[0031] Figure 8 Schematic structural diagram of a fixing assembly of a bacterial culture detection device proposed by the present utility model.

[0032] In the figure: 1, culture chamber; 2, fixing assembly; 3, top cover; 4, concentration detection assembly; 5, high-concentration culture solution tank; 6, electric control ball valve; 7, automatic control ball valve; 8, liquid supplement pipe; 9, suction pipe; 10, cylinder block; 11, electrode head; 12, piston 1; 13, reciprocating drive assembly; 14, valve housing 1; 15, ball core 1; 16, valve stem 1; 17, spring 1; 18, spiral groove 1; 19, limit protrusion 1; 20, electromagnet; 21, permanent magnet; 22, slider 1; 23, fixed housing; 24, motor; 25, support plate; 26, crankshaft 1; 27, crankshaft 2; 28, connecting rod 1; 29, ball core 2; 30, valve housing 2; 31, spiral groove 2; 32, connecting rod 2; 33, slider 2; 34, limit protrusion 2; 35, valve stem 2; 36, screw rod; 37, knob; 38, flange; 39, suction cup; 40, piston 2; 41, spring 2. Specific embodiments

[0033] The technical solutions of the present utility model will be further described in detail below in conjunction with specific embodiments.

[0034] The embodiments of the present utility model will be described in detail below. The examples of the embodiments are shown in the drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary and are only used to explain the present utility model and should not be construed as a limitation of the present utility model.

[0035] Embodiment 1:

[0036] A bacterial culture detection device, as Figures 1-8 shown, includes a culture chamber 1, in which bacteria are cultured by a culture solution. The outer wall of the top of the culture chamber 1 is fixed with a top cover 3 by bolts. A concentration detection assembly 4 is arranged on the top of the top cover 3. The top of the top cover 3 is connected to a high-concentration culture solution tank 5 through a liquid supplement pipe 8, and an electric control ball valve 6 is installed on the liquid supplement pipe 8.

[0037] The concentration detection component 4 includes a suction pipe 9, a cylinder body 10, an electrode head 11, and a first piston 12. The suction pipe 9 is fixed to the outer wall of the top cover 3, and the bottom of the suction pipe 9 penetrates through the top cover 3 and is placed inside the culture chamber 1. The first piston 12 is slidably connected to the inner wall of the cylinder body 10. Two electrode heads 11 are fixedly embedded on the inner side wall of the cylinder body 10. The electrode heads 11 are connected to a power source, and a reciprocating drive component 13 for reciprocally driving the first piston 12 is provided on the top of the first piston 12.

[0038] When the device is in use, nutrient solution can be prepared in the culture chamber 1, and then bacteria are inoculated into the culture chamber 1. During the culture process, the reciprocating drive component 13 drives the first piston 12 to reciprocate. When the first piston 12 moves upward, negative pressure can be generated in the cylinder body 10, and the nutrient solution in the culture chamber 1 is sucked into the cylinder body 10 through the suction pipe 9. Since the number of bacterial cells increases, the contribution of the charges on the cell membrane and the ion concentration difference inside and outside the cell to the conductivity of the solution also increases. At the same time, the number of ions produced by bacterial metabolism also increases, thereby improving the conductivity. The conductivity is detected by the circuit of the electrode head 11. When the conductivity is high, it means that the number of bacteria is large, so that the electric control ball valve 6 is opened, and the high-concentration nutrient solution in the high-concentration culture solution tank 5 will flow along the liquid supplement pipe 8 into the inside of the top cover 3 for supplementation to increase the concentration.

[0039] In this device, by utilizing the ion conductivity characteristics of the inside and outside of the bacterial cell membrane and the ions of bacterial metabolism, and using the electrode head 11 to sense the conductivity, when the number of bacteria increases, high-concentration nutrient solution can be automatically supplemented into the culture chamber 1 to prevent malnutrition.

[0040] The bottom of the suction pipe 9 is inserted below the nutrient solution in the culture chamber 1 and is not located at the bottom of the culture chamber 1.

[0041] When the suction pipe 9 sucks the nutrient solution, the liquid level of the nutrient solution in the culture chamber 1 will drop, so that the bottom of the suction pipe 9 is gradually exposed to the place without nutrient solution, and then air is sucked in. By using air insulation, the nutrient solution in the cylinder body 10 and the suction pipe 9 is insulated from the nutrient solution in the culture chamber 1.

[0042] In this device, by restricting the height of the suction pipe 9, when detecting, a small amount of nutrient solution can be sucked, and the remaining nutrient solution in the culture chamber 1 will not conduct electricity, so as to prevent the bacteria from being affected in the potential condition during detection and affecting reproduction.

[0043] To solve the lifting drive problem; as Figure 6As shown in the figure, the reciprocating drive assembly 13 includes a support plate 25 fixed to the top of the top cover 3 and a motor 24 bolted to the side wall of the support plate 25. The outer wall of the output shaft of the motor 24 is successively fixed with a first crankshaft 26 and a second crankshaft 27. The eccentric part of the second crankshaft 27 is rotatably connected to a first connecting rod 28, and the bottom of the first connecting rod 28 is rotatably connected to the top outer wall of the first piston 12.

[0044] When the motor 24 is started, it can drive the second crankshaft 27 to rotate, thereby driving the first piston 12 to reciprocate up and down through the first connecting rod 28.

[0045] To solve the problem of automatic liquid supplement; as Figure 5 As shown in the figure, the electrically controlled ball valve 6 includes a first valve housing 14 and a first ball core 15 rotatably connected to the inside of the first valve housing 14 through a first valve stem 16. A fixed housing 23 is welded to the side wall of the first valve housing 14. A first slider 22 is slidably connected to the inner wall of the fixed housing 23. A first spiral groove 18 is formed on the outer wall of the first valve stem 16, and a first limiting protrusion 19 that is movably and limitedly matched with the first spiral groove 18 is fixed to the inner wall of the first slider 22.

[0046] A permanent magnet 21 is fixed to the side wall of the first slider 22, and an electromagnet 20 that cooperates with the permanent magnet 21 is fixed to the inner wall of the fixed housing 23. A first spring 17 is buckled to the outer wall of the other side of the first slider 22, and the other end of the first spring 17 is buckled to the outer wall of the first valve housing 14.

[0047] The electromagnet 20 is connected in series to the circuit of the electrode head 11.

[0048] When there are fewer bacteria and the conductivity of the solution is weaker, the internal resistance of the solution is larger. At this time, the current in the electromagnet 20 is smaller, and its field strength is smaller, so that the magnetic repulsion force between the electromagnet 20 and the permanent magnet 21 cannot overcome the elastic force of the first spring 17, and the first slider 22 does not move, and the first ball core 15 is in the closed state, and no liquid is supplemented at this time. When there are more bacteria and the conductivity of the solution is stronger, the internal resistance of the solution is smaller. At this time, the current in the electromagnet 20 increases, and the magnetic force between the electromagnet 20 and the permanent magnet 21 overcomes the elastic force of the first spring 17 to make the first slider 22 move. Thus, by using the cooperation between the first limiting protrusion 19 and the first spiral groove 18, the first valve stem 16 drives the first ball core 15 to rotate, and the first ball core 15 opens to supplement the liquid. Moreover, the more bacteria there are, the smaller the internal resistance of the solution is, the greater the magnetic field strength of the electromagnet 20 is, the larger the rotation angle of the first ball core 15 is, the larger the opening degree is, and the faster the liquid supplement speed is.

[0049] In this device, through the targeted design of the electrically controlled ball valve 6, on the one hand, based on the electrical measurement of the bacteria concentration and combined with the circuit, the automatic liquid supplement process is realized. On the other hand, the control of the liquid supplement speed can also be achieved according to the relationship between the conductivity and the bacteria concentration.

[0050] To solve the reliability problem; such as Figure 1 , 7 As shown, a self-controlled ball valve 7 is further provided on the liquid replenishing pipe 8. The self-controlled ball valve 7 includes a second valve housing 30 and a second ball core 29 rotatably connected to the inner wall of the second valve housing 30 through a second valve rod 35. A second spiral groove 31 is formed on the outer wall of the second valve rod 35. A second slider 33 is slidably connected to the outer wall of the second valve rod 35. A second limiting protrusion 34 fixedly connected to the inner wall of the second slider 33 is movably and limitedly engaged with the second spiral groove 31. The outer wall of the second slider 33 is rotatably connected to one end of a second connecting rod 32, and the other end of the second connecting rod 32 is rotatably connected to the eccentric position of a first crankshaft 26.

[0051] When the first crankshaft 26 and the second crankshaft 27 rotate to the position where the first piston 12 is at the highest position, at this time, the first crankshaft 26 will also pull the second connecting rod 32 to make the second ball core 29 in the open state. When in other positions, the second ball core 29 is in the closed state.

[0052] Since the electrode head 11 contacts the nutrient solution only when the first piston 12 reaches a relatively high position, and the bacterial concentration detection will be carried out at this time. If the electric control ball valve 6 is accidentally started before the detection, it will lead to low detection accuracy. Based on this, the device is provided with a self-controlled ball valve 7 connected in series with the electric control ball valve 6, and the opening and closing of the self-controlled ball valve 7 are controlled by the position of the first crankshaft 26, so that only when the first piston 12 is at a relatively high position, the self-controlled ball valve 7 will open to allow the nutrient solution to pass through. Otherwise, even if the electric control ball valve 6 is opened, the nutrient solution will not pass through, thereby increasing the reliability of liquid replenishment.

[0053] When this embodiment is in use, the nutrient solution can be prepared in the culture chamber 1, and then bacteria are inoculated into the culture chamber 1. During the culture process, the reciprocating drive assembly 13 drives the piston 12 to reciprocate. When the piston 12 moves upward, a negative pressure can be generated in the cylinder 10, and the nutrient solution in the culture chamber 1 is sucked into the cylinder 10 through the straw 9. Since the number of bacterial cells increases, the contribution of the charge on the cell membrane and the ion concentration difference inside and outside the cell to the conductivity of the solution also increases. At the same time, the number of ions produced by bacterial metabolism also increases, thereby improving the conductivity. The conductivity is detected using the circuit of the electrode head 11. When the conductivity is high, it means that the number of bacteria is large, so that the electronically controlled ball valve 6 is opened, and the high-concentration nutrient solution in the high-concentration culture solution tank 5 will flow along the liquid supplement pipe 8 into the inside of the top cover 3 for supplementation to increase the concentration. When the straw 9 sucks in the nutrient solution, the liquid level of the nutrient solution in the culture chamber 1 will drop, so that the bottom of the straw 9 is gradually exposed to the place without nutrient solution, and then air is sucked in. Using air insulation, the nutrient solution in the cylinder 10 and the straw 9 is insulated from the nutrient solution in the culture chamber 1. When there are fewer bacteria and the conductivity of the solution is weak, the internal resistance of the solution is large. At this time, the current in the electromagnet 20 is small, and its field strength is small, so that the magnetic repulsive force between the electromagnet 20 and the permanent magnet 21 cannot overcome the elastic force of the spring 17, and the slider 22 does not move, and the ball core 15 is in a closed state. At this time, no liquid is supplemented. When there are more bacteria and the conductivity of the solution is strong, the internal resistance of the solution is small. At this time, the current in the electromagnet 20 increases, and the magnetic force between the electromagnet 20 and the permanent magnet 21 overcomes the elastic force of the spring 17 to make the slider 22 move. Thus, using the cooperation of the limit protrusion 19 and the spiral groove 18, the valve stem 16 drives the ball core 15 to rotate, and the ball core 15 is opened for liquid supplementation. Moreover, the more bacteria there are, the smaller the internal resistance of the solution, the greater the magnetic field strength of the electromagnet 20, the greater the rotation angle of the ball core 15, the greater the opening degree, and the faster the liquid supplementation speed.

[0054] Embodiment 2:

[0055] A bacterial culture detection device, as Figure 1 、 8 shown, to solve the stability problem; on the basis of Embodiment 1, the following improvements are made in this embodiment: A fixing component 2 is arranged on the outer side of the culture chamber 1. The fixing component 2 includes a flange 38, a suction cup 39, and a piston 40 slidably connected inside the suction cup 39. A spring 41 is buckled on the top of the suction cup 39, and the other end of the spring 41 is buckled on the bottom outer wall of the flange 38. The flange 38 is fixed to the outside of the culture chamber 1 by bolts. A screw 36 is threadedly connected to the inner wall of the flange 38. A knob 37 is fixed to the top of the screw 36, and the other end of the screw 36 is rotatably connected to the piston 40.

[0056] When this embodiment is in use, the suction cup 39 is attached to the bearing surface. At this time, the second spring 41 exerts an upward force on the flange 38, and negative pressure is generated in the suction cup 39, thereby adsorbing the device to the desktop. When moving, rotate the knob 37 to make the second piston 40 move downward, and the negative pressure in the suction cup 39 disappears, allowing it to be moved.

[0057] The above are only the preferred specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution of the present invention and its inventive concept, making equivalent replacements or changes, should be covered within the protection scope of the present invention.

Claims

1. A bacterial culture detection device, comprising a culture chamber (1), characterized in that, The interior of the culture chamber (1) is cultured with bacteria through a culture solution. The outer wall of the top of the culture chamber (1) is fixed with a top cover (3) by bolts. A concentration detection component (4) is arranged on the top of the top cover (3). The top of the top cover (3) is connected to a high-concentration culture solution tank (5) through a liquid replenishing pipe (8). An electric control ball valve (6) is installed on the liquid replenishing pipe (8); the concentration detection component (4) includes a suction pipe (9), a cylinder body (10), an electrode head (11), and a first piston (12). The suction pipe (9) is fixed to the outer wall of the top of the top cover (3), and the bottom of the suction pipe (9) penetrates through the top cover (3) and is placed inside the culture chamber (1). The first piston (12) is slidably connected to the inner wall of the cylinder body (10). Two electrode heads (11) are fixedly embedded on the inner side wall of the cylinder body (10). The electrode head (11) is connected to a power supply, and a reciprocating drive component (13) for reciprocally driving the first piston (12) is arranged on the top of the first piston (12).

2. The bacterial culture detection device according to claim 1, wherein The bottom of the suction pipe (9) is inserted below the nutrient solution in the culture chamber (1) and is not located at the bottom of the culture chamber (1).

3. The bacterial culture detection device according to claim 1, wherein, The reciprocating drive component (13) includes a support plate (25) fixed to the top of the top cover (3) and a motor (24) fixed to the side wall of the support plate (25) by bolts. The outer wall of the output shaft of the motor (24) is successively fixed with a first crankshaft (26) and a second crankshaft (27). The eccentric part of the second crankshaft (27) is rotatably connected to a first connecting rod (28). The bottom of the first connecting rod (28) is rotatably connected to the outer wall of the top of the first piston (12).

4. A bacterial culture detection device according to claim 1, characterized in that, The electric control ball valve (6) includes a first valve housing (14) and a first ball core (15) rotatably connected to the inside of the first valve housing (14) through a first valve rod (16). A fixed housing (23) is welded to the side wall of the first valve housing (14). A first slider (22) is slidably connected to the inner wall of the fixed housing (23). A first spiral groove (18) is formed on the outer wall of the first valve rod (16). A first limiting protrusion (19) which is movably and limitingly matched with the first spiral groove (18) is fixed to the inner wall of the first slider (22).

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