Dyeing module and immunofluorescence stainer

Abstract

The utility model relates to a staining module and an immunofluorescence staining instrument, and the staining module comprises a mechanical arm module which is provided with a liquid suction and injection needle capable of moving along a preset direction; the glass slide module is provided with a cleaning groove and a plurality of glass slide grooves, the glass slide grooves are used for bearing glass slides, reaction holes are formed in the glass slides, and cleaning liquid is stored in the cleaning groove; when the glass slides are arranged in one or more glass slide grooves, the liquid suction and injection needle can enter the reaction hole and the cleaning groove of each glass slide in the movement process of the liquid suction and injection needle. After the liquid suction and injection needle enters the reaction hole of one glass slide and before the liquid suction and injection needle enters the reaction holes of the other glass slides, the liquid suction and injection needle enters the cleaning tank firstly, and the liquid suction and injection needle is cleaned through the cleaning liquid in the cleaning tank, so that pollution sources possibly carried by the liquid suction and injection needle are removed; the influence on washing among the glass slides is avoided, and the dyeing quality of a sample is improved.

Description

Technical Field

[0001] The present application relates to the technical field of medical testing equipment, and particularly to a staining module and an immunofluorescence staining instrument. Background Art

[0002] Immunofluorescence staining technology is a protein and other molecular detection technology developed on the basis of immunology, combined with biochemical technology and microscopy technology. Due to the characteristics of strong specificity, high sensitivity, fast speed, and easy observation, this technology is widely used in the fields of cell biology, microbiology, and clinical diagnosis.

[0003] An immunofluorescence staining instrument can be used for automated experimental steps such as sample dilution, pipetting, incubation, and washing, thereby improving the quality and efficiency of fluorescence staining. Among them, the experimental steps of incubation and washing are usually carried out in the staining module of the immunofluorescence staining instrument. When the staining module is washing, the injection needle and the suction needle need to enter the slide holes of each slide respectively, and perform the operations of injecting liquid and sucking liquid into the slide holes. If there is a pollution source in one of the slide holes, the injection needle and the suction needle will bring the pollution source to other slide holes during the movement, causing pollution of the slide holes, so that the consistency of the cleaning quality of each slide hole cannot be guaranteed, thus affecting the experimental results. Summary of the Invention

[0004] Based on this, in view of the problem that the consistency of the cleaning quality of each slide hole cannot be guaranteed in the experimental step of washing of the immunofluorescence staining instrument, it is necessary to provide a staining module and an immunofluorescence staining instrument.

[0005] A staining module includes:

[0006] A robotic arm module having an injection / suction needle capable of moving along a preset direction;

[0007] A slide module having a cleaning tank and a plurality of slide slots, each of the slide slots being used to carry the slide, reaction holes being provided on the slide, and the cleaning tank being used to store cleaning liquid;

[0008] When one or more of the slide slots are provided with the slides, during the movement of the injection / suction needle, the injection / suction needle can enter the reaction holes of each slide and the cleaning tank.

[0009] In one embodiment, the slide module includes a fixed seat, a guide bar, and a tray. The guide bar is installed on the fixed seat, the tray is detachably installed on the guide bar, and the cleaning tank and all the slide slots are provided on the tray.

[0010] In one embodiment, a guiding groove is formed on the guiding bar, and the tray can enter the guiding groove and move along the longitudinal direction of the guiding groove;

[0011] A positioning structure is formed on the guiding bar. During the movement of the tray in the guiding groove, the positioning structure is located on the movement path of the tray and can be locked with the tray;

[0012] The slide module further includes a position detection member, which is communicatively connected to the robotic arm module. When the positioning structure is locked with the tray, the position detection member is triggered.

[0013] In one embodiment, the staining module further includes a dilution module, and the dilution module includes a plurality of dilution plates, and all the dilution plates are arranged on the fixing seat;

[0014] And / or, a plurality of upright columns are further arranged on the fixing seat.

[0015] In one embodiment, the robotic arm module includes a first motion assembly, a second motion assembly, and a third motion assembly. The second motion assembly is arranged on the first motion assembly and can move along a first direction under the action of the first motion assembly. The third motion assembly is arranged on the second motion assembly and can move along a second direction under the action of the second motion assembly;

[0016] The liquid injection and suction needle is arranged on the third motion assembly and can move along the third direction under the action of the third motion assembly. The first direction, the second direction, and the third direction intersect pairwise and are not coplanar.

[0017] In one embodiment, the third motion assembly includes a driving member, a cam, a mounting assembly, and a movable assembly. The driving member is used to drive the cam to rotate, and the rotation axis direction of the cam intersects the third direction.

[0018] The movable assembly is movably mounted on the mounting assembly along the third direction, and the movable assembly is in contact and cooperation with the outer peripheral surface of the cam. The liquid injection and suction needle is located on the movable assembly.

[0019] In one embodiment, the outer peripheral surface of the cam has a highest point and a lowest point, and the distance from the highest point to the rotation axis is greater than the distance from the lowest point to the rotation axis;

[0020] The third motion assembly further includes an elastic member, which is elastically arranged between the movable assembly and the guiding member and can be deformed along the third direction;

[0021] When the contact point between the movable component and the cam moves from the lowest point to the highest point, the elastic component accumulates elastic force. When the contact point between the movable component and the cam moves from the highest point to the lowest point, the elastic component releases elastic force, and the elastic force is used to make the movable component press against the cam.

[0022] In one embodiment, the mounting component includes a mounting plate and a guiding block. The guiding block is arranged on the mounting plate and is provided with a guiding hole extending longitudinally along the third direction.

[0023] The movable component includes a roller, a connecting plate, and a connecting column arranged in sequence along the third direction. The roller is movably mounted on the mounting plate along the third direction, and the outer peripheral surface of the roller is in contact with the outer peripheral surface of the cam. One end of the connecting plate in the third direction abuts against the roller, and the other end is connected to one end of the connecting column. The connecting column passes through the guiding hole, and the other end is provided with the injection and suction needle. The elastic component is sleeved on the connecting column, and both ends of the elastic component respectively abut against the connecting plate and the guiding block.

[0024] In one embodiment, the staining module further includes a vision module. The vision module is arranged on the third moving component and is used to identify the slide in the slide slot.

[0025] An immunofluorescence staining instrument includes the staining module described in any one of the above.

[0026] The above staining module has at least the following advantages:

[0027] Using cam drive to realize the up and down movement of the injection and suction needle, the overall structure is light, high-precision lifting can be achieved, accurate washing can be realized, and the staining quality can be improved.

[0028] A positioning structure and a in-place detection component are arranged on the slide module, so that the position of the slide on the tray can be accurately positioned, and whether the tray is in place can be detected through the in-place detection component, ensuring that the slide has reached the designed position and improving the staining quality.

[0029] A cleaning tank for storing cleaning liquid is arranged on the slide module, and the injection and suction needle can be cleaned to avoid the injection and suction needle carrying pollution sources and affecting the washing between slides, thus improving the staining quality.

[0030] It is equipped with a vision module, so that it can identify whether there is a slide in the slide slot and the coding information on the slide, so that the injection and suction needle only incubates and cleans the positions with slides, preventing misoperation, recording the slide number for convenient traceability, being more flexible, and improving the efficiency.

[0031] The first motion component, the second motion component, and the third motion component of the staining module can achieve three-axis linkage, accurately move to the reaction wells of the slide for operation, reduce the motion time, and improve the efficiency. Description of the Drawings

[0032] Figure 1 It is a schematic structural diagram of the staining module in some embodiments of the present application.

[0033] Figure 2 For Figure 1 It is a schematic structural diagram of the slide module in the embodiment.

[0034] Figure 3 For Figure 1 It is a schematic structural diagram of the third motion component in the embodiment.

[0035] Description of the Reference Numerals:

[0036] Robotic arm module 10; aspiration and injection needle 11; aspiration needle 111; injection needle 112; first motion component 12; second motion component 13; third motion component 14;

[0037] Slide module 20; cleaning tank 21; slide slot 22; slide 221; reaction well 223; fixing seat 23; tray 24; guiding groove 241; guiding bar 242; in-place detection member 25;

[0038] Dilution module 30; dilution plate 31; column 32; vision module 33;

[0039] Driver 40; cam 41; mounting assembly 42; mounting plate 421; guiding block 423; guiding hole 424; movable assembly 43; roller 431; connecting plate 432; connecting column 433; highest point 44; lowest point 45; elastic member 46;

[0040] First direction X; second direction Y; third direction Z. Detailed Embodiments

[0041] To make the above objects, features, and advantages of the present application more obvious and understandable, the following will describe the detailed embodiments of the present application with reference to the accompanying drawings. Many specific details are set forth in the following description in order to fully understand the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the connotation of the present application. Therefore, the present application is not limited by the specific embodiments disclosed below.

[0042] In the description of the present application, it should be understood that if there are terms such as "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., the orientation or positional relationship indicated by these terms is based on the orientation or positional relationship shown in the drawings. These are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present application.

[0043] In addition, if there are terms such as "first" and "second", these terms are only used for descriptive purposes and should not be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include at least one of such features. In the description of the present application, if there is a term "plurality", the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise specifically and clearly defined.

[0044] In the present application, unless otherwise clearly defined and limited, if there are terms such as "mounted", "connected", "connected to", "fixed", etc., these terms should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or integrated; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two elements or the interaction relationship between two elements, unless otherwise clearly limited. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific circumstances.

[0045] In the present application, unless otherwise clearly defined and limited, if there is a description such as a first feature being "on" or "under" a second feature, the meaning may be that the first and second features are in direct contact, or the first and second features are indirectly in contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature has a higher horizontal height than the second feature. The first feature being "under", "beneath" and "underneath" the second feature may be that the first feature is directly below or obliquely below the second feature, or simply means that the first feature has a lower horizontal height than the second feature.

[0046] It should be noted that if an element is referred to as "fixed to" or "disposed on" another element, it can be directly on the other element or there may be an intermediate element. If an element is considered to be "connected" to another element, it can be directly connected to the other element or there may be an intermediate element at the same time. If present, the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in this application are only for illustrative purposes and do not represent the only implementation.

[0047] Referring to Figure 1 and Figure 2 , a staining module provided by an embodiment of the present application includes a robotic arm module 10 and a slide module 20. The robotic arm module 10 includes a pipetting needle 11 that can move along a preset direction. The pipetting needle 11 refers to a needle-like structure that can perform operations of sucking liquid and injecting liquid. This needle-like structure can be composed of two needle tubes. As Figure 3 shown, this needle-like structure is composed of a liquid suction needle 111 and a liquid injection needle 112. In some other embodiments, the pipetting needle 11 can also have only one needle tube, and by connecting the needle tube to different devices, the operations of sucking liquid and injecting liquid can be achieved.

[0048] The slide module 20 has a cleaning tank 21 and a plurality of slide slots 22. The cleaning tank 21 is used to store cleaning liquid, and each slide slot 22 is used to carry a slide 221. A reaction hole 223 is provided on the slide 221. When one or more slide slots 22 are provided with slides 221, during the movement of the pipetting needle 11, it can enter the reaction hole 223 of each slide 221 and inject cleaning liquid into the reaction hole 223 and suck away the waste liquid after washing.

[0049] After the pipetting needle 11 enters the reaction hole 223 of one of the slides 221, in order to prevent the pipetting needle 11 from being contaminated by the possible pollution sources in the reaction hole 223, resulting in the contamination of other reaction holes 223 when the pipetting needle 11 enters the reaction holes 223 of other slides 221. During the movement of the pipetting needle 11, the pipetting needle 11 can also enter the cleaning tank 21. When the cleaning tank 21 stores cleaning liquid, after the pipetting needle 11 enters the cleaning tank 21, the cleaning liquid will clean the pipetting needle 11 and remove the possible pollution on the pipetting needle 11.

[0050] Thus, after the injection and aspiration needle 11 enters the reaction well 223 of one of the glass slides 221, before the injection and aspiration needle 11 enters the reaction wells 223 of other glass slides 221, the injection and aspiration needle 11 will first enter the cleaning tank 21, and the cleaning liquid in the cleaning tank 21 will clean the injection and aspiration needle 11 to remove the possible pollution sources carried by the injection and aspiration needle 11, avoiding affecting the washing between the glass slides 221 and improving the staining quality of the sample.

[0051] In some embodiments of the present application, the glass slide module 20 includes a fixing base 23, a guide bar 242 and a tray 24. The guide bar 242 is installed on the fixing base 23, and the tray 24 is detachably installed on the guide bar 242. The cleaning tank 21 and all the glass slide grooves 22 are formed on the tray 24 to carry the glass slides 221 through the tray 24. In the actual use process, the glass slides 221 can be first placed on the tray 24, and the tray 24 is installed on the fixing base 23 through the guide bar 242, so that the reaction wells 223 in the glass slides 221 on the tray 24 can be washed by the injection and aspiration needle 11 to remove the impurities that did not participate in the reaction in the reaction wells 223. After the washing is completed, the reaction wells 223 can be sealed, and the tray 24 is removed from the guide bar 242, so that all the glass slides 221 are transferred to the reader through the tray 24 for interpretation.

[0052] In some embodiments, a guide groove 241 and an opening communicating with the guide groove 241 are formed on the guide bar 242. The tray 24 can enter the guide groove 241 and move along the longitudinal direction of the guide groove 241. Specifically, two guide bars 242 are provided on the fixing base 23, and the two guide bars 242 are spaced apart. The guide groove 241 is formed on each guide bar 242. When the tray 24 enters the two guide grooves 241 from one end of the two guide bars 242, the edge of the tray 24 is located in the two guide grooves 241, so that the tray 24 can only move along the longitudinal direction of the guide groove 241, playing a role in limiting the movement of the tray 24.

[0053] Among them, a positioning structure is formed on the guide bar 242. During the movement of the tray 24 in the guide groove 241, the positioning structure is located on the movement path of the tray 24 and can be locked with the tray 24, so as to fix the tray 24 in the guide bar 242, avoiding the tray 24 from shaking during the washing of the reaction wells 223 in the glass slides 221 by the injection and aspiration needle 11, which may cause equipment damage.

[0054] Among them, the slide module 20 further includes a position detection member 25. The position detection member 25 is communicatively connected to the robotic arm module 10. When the positioning structure and the tray 24 are locked to each other, the position detection member 25 is triggered. The position detection member 25 sends a signal to the robotic arm module 10. After the robotic arm module 10 receives the signal, it will drive the pipetting needle 11 to act. That is to say, only when the tray 24 and the positioning structure are fixed to each other, and at this time the tray 24 has reached the designed position, will the robotic arm module 10 start to wash the reaction holes 223 of the slide 221 on the tray 24, avoiding the situation where the tray 24 is not installed in place and the robotic arm module 10 acts, resulting in equipment damage, improving the staining quality, and at the same time being able to avoid the pipetting needle 11 starting to wash and stain before moving to the designed precise position.

[0055] It can be understood that the positioning structure can be a positioning pin, a positioning buckle, a positioning hole, or other structures that can form a positioning fit with the tray 24.

[0056] Specifically in some embodiments, the staining module further includes a dilution module 30. The dilution module 30 includes a plurality of dilution plates 31. All the dilution plates 31 are arranged on the fixing base 23. The dilution plates 31 are used to fully mix the diluent and the sample with the sample, so as to obtain a uniformly diluted sample. Then, the diluted sample is dispensed into the reaction holes 223 of the slide 221 through the pipetting structure. And setting the dilution module 30 and the slide 221 module on the fixing base 23 can reduce the distance between the two, facilitating the operation of the pipetting structure.

[0057] Among them, a plurality of columns 32 are further arranged on the fixing base 23. The plurality of columns 32 can be used to support the fixing base 23, and the fixing base 23 can also be installed in other devices for use. At this time, the columns 32 can play a positioning role.

[0058] In some embodiments of the present application, refer to Figure 1 and Figure 3 , in order to move the pipetting needle 11, the robotic arm module 10 includes a first motion component 12, a second motion component 13, and a third motion component 14. The second motion component 13 is arranged on the first motion component 12 and can move along the first direction X under the action of the first motion component 12. The third motion component 14 is arranged on the second motion component 13 and can move along the second direction Y under the action of the second motion component 13. The pipetting needle 11 is arranged on the third motion component 14 and can move along the third direction Z under the action of the third motion component 14. The first direction X, the second direction Y, and the third direction Z intersect pairwise and are not coplanar.

[0059] Thus, when the second moving component 13 moves along the first direction X, the third moving component 14 and the liquid injection and suction needle 11 will move together along the first direction X. When the third moving component 14 moves along the second direction Y, it will drive the liquid injection and suction needle 11 to move along the second direction Y. The liquid injection and suction needle 11 can achieve movement in the third direction Z through the third moving component 14. Thus, the first moving component 12, the second moving component 13, and the third moving component 14 can enable the liquid injection and suction needle 11 to perform three-dimensional movement, so that the liquid injection and suction needle 11 can accurately move into the reaction holes 223 of each glass slide 221 and the cleaning tank 21, achieving the effect of reducing the movement time and improving the efficiency.

[0060] In some embodiments, the first moving component 12 and the second moving component 13 can adopt driving methods such as linear modules, cylinder driving, screw driving, and belt driving. The third moving component 14 includes a driving member 40, a cam 41, a mounting component 42, and a movable component 43. The driving member 40 is used to drive the cam 41 to rotate, and the rotation axis direction of the cam 41 intersects with the third direction Z. The movable component 43 is movably mounted on the mounting component 42 along the third direction Z, and the movable component 43 is in contact and cooperation with the outer peripheral surface of the cam 41. The liquid injection and suction needle 11 is located on the movable component 43.

[0061] Since the distance between the outer peripheral surface of the cam 41 and the rotation axis is not fixed, when the cam 41 rotates, under the action of the outer peripheral surface of the cam 41, the distance of the movable component 43 relative to the rotation axis will also change, thereby realizing the movement of the movable component 43 in the third direction Z, and further driving the liquid injection and suction needle 11 to move in the third direction Z. Using the cam 41 transmission to realize the up and down movement of the washing needle, its structure is light, it can achieve high-precision lifting, accurate washing, and improve the staining quality.

[0062] Further, the outer peripheral surface of the cam 41 has a highest point 44 and a lowest point 45. The distance from the highest point 44 to the rotation axis is greater than the distance from the lowest point 45 to the rotation axis. The third moving component 14 further includes an elastic member 46. The elastic member 46 is elastically arranged between the movable component 43 and the guiding member and can be deformed along the third direction Z.

[0063] When the contact point between the movable component 43 and the cam 41 moves from the lowest point 45 to the highest point 44, the elastic member 46 accumulates elastic force. When the contact point between the movable component 43 and the cam 41 moves from the highest point 44 to the lowest point 45, the elastic member 46 releases the elastic force. The released elastic force is used to make the movable component 43 press against the cam 41, so that the movable component 43 can always be in contact with the outer peripheral surface of the cam 41 through the elastic member 46.

[0064] Specifically, in some embodiments, the mounting assembly 42 includes a mounting plate 421 and a guiding block 423. The guiding block 423 is disposed on the mounting plate 421 and has a guiding hole 424 extending longitudinally along the third direction Z. The movable assembly 43 includes a roller 431, a connecting plate 432, and a connecting column 433 arranged in sequence along the third direction Z. The roller 431 is movably mounted on the mounting plate 421 along the third direction Z, and the outer peripheral surface of the roller 431 is in contact with the outer peripheral surface of the cam 41. One end of the connecting plate 432 in the third direction Z abuts against the roller 431, and the other end is connected to one end of the connecting column 433. The connecting column 433 passes through the guiding hole 424, and a liquid injection needle 11 is provided at the other end. An elastic member 46 is sleeved on the connecting column 433, and both ends of the elastic member 46 respectively abut against the connecting plate 432 and the guiding block 423.

[0065] When the cam 41 rotates, the outer peripheral surface of the cam 41 contacts the outer peripheral surface of the roller 431. Under the action of the cam 41, the roller 431 moves in the third direction Z, and the roller 431 itself can also rotate, so that the roller 431 reduces the frictional force between the roller 431 and the cam 41 through its own rotation.

[0066] Wherein, when the roller 431 is disposed below the cam 41, when the contact point between the roller 431 and the outer peripheral surface of the cam 41 moves from the lowest point 45 to the highest point 44, the cam 41 drives the roller 431 to move downward, so that the connecting plate 432 approaches the guiding block 423, and finally the elastic member 46 is gradually compressed. When the contact point between the roller 431 and the outer peripheral surface of the cam 41 moves from the highest point 44 to the lowest point 45, the elastic member 46 gradually recovers, and the elastic member 46 pushes the connecting plate 432 and the roller 431 to move upward, so that the roller 431 always remains in contact with the cam 41. Until after the contact point between the roller 431 and the outer peripheral surface of the cam 41 passes through the lowest point 45, the cam 41 begins to drive the roller 431 to move downward and continue to compress the spring. Repeating this way can realize the movement of the connecting plate 432 in the third direction Z, and finally drive the connecting column 433 and the liquid injection needle 11 to move in the third direction Z through the connecting plate 432.

[0067] It should be noted that in other embodiments, the elastic member, the roller, etc. may not be provided. The movable assembly 43 can be directly mounted on the outer surface of the cam 41 slidably along the cam 41 through a chute or the like at one end in the third direction, so that the movable assembly 43 is always connected to the outer surface of the cam 41. During the movement of the cam 41, it will drive the movable assembly 43 to move in the third direction.

[0068] In some embodiments of the present application, the staining module further includes a vision module 33. The vision module 33 is disposed on the third motion component 14 to move along with the injection and aspiration needle 11 on the third motion component 14 in the first direction X and the second direction Y. The vision module 33 can identify the glass slides 221 in the glass slide slots 22, so as to identify which glass slide slots 22 contain glass slides 221 and which do not. The vision module 33 feeds back the detection result to the robotic arm module 10, and the robotic arm module 10 drives the injection and aspiration needle 11 according to the detection result, so that the injection and aspiration needle 11 only incubates and washes the positions where there are glass slides 221, preventing misoperation.

[0069] Furthermore, the vision module 33 can also be used to identify the coding information on the glass slide 221, so as to record the coding information on the glass slide 221 for convenient traceability, making the experiment more flexible and efficient.

[0070] The embodiments of the present application also provide an immunofluorescence staining instrument, including the staining module in any of the above embodiments. Since this immunofluorescence staining instrument includes all the technical features of the above staining module, therefore, this immunofluorescence staining instrument has all the technical effects of the above staining module, which will not be elaborated here.

[0071] The above staining module has at least the following advantages:

[0072] The up and down movement of the injection and aspiration needle 11 is realized by using the cam 41 transmission. The overall structure is light, and high-precision lifting and accurate washing can be achieved, improving the staining quality;

[0073] A positioning structure and a in-place detection member 25 are provided on the glass slide module 20, so as to accurately position the position of the glass slide 221 on the tray 24 and detect whether the tray 24 is in place through the in-place detection member 25, ensuring that the glass slide has reached the designed position and improving the staining quality;

[0074] A cleaning tank 21 for storing cleaning liquid is provided on the glass slide module 20, which can clean the injection and aspiration needle 11, avoiding the injection and aspiration needle 11 carrying pollution sources and affecting the washing between the glass slides 221, and improving the staining quality;

[0075] It is equipped with a vision module, so as to be able to identify whether there is a glass slide 221 in the glass slide slot 22 and the coding information on the glass slide 221, so that the injection and aspiration needle 11 only incubates and cleans the positions where there are glass slides 221, preventing misoperation, recording the glass slide number for convenient traceability, being more flexible and improving the efficiency.

[0076] The first motion component 12, the second motion component 13 and the third motion component 14 of the staining module can achieve three-axis linkage and accurately move to the reaction holes 223 of the glass slide 221 to work, reducing the motion time and improving the efficiency.

[0077] The technical features of the above-described embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered to be within the scope described in this specification.

[0078] The above-described embodiments only represent several implementation manners of the present application. The description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the patent application. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present application, several modifications and improvements can still be made, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application shall be subject to the appended claims.

Claims

1. A dyeing module, characterized in that The staining module includes: A robotic arm module (10) having a liquid suction and injection needle (11) capable of moving along a preset direction; A glass slide module (20) having a cleaning tank (21) and a plurality of glass slide slots (22). Each of the glass slide slots (22) is used to carry a glass slide (221). A reaction hole (223) is provided on the glass slide (221), and a cleaning liquid is stored in the cleaning tank (21); When one or more of the glass slide slots (22) are provided with the glass slides (221), during the movement of the liquid suction and injection needle (11), the liquid suction and injection needle (11) can enter the reaction hole (223) of each glass slide (221) and the cleaning tank (21).

2. The dyeing module according to claim 1, wherein The glass slide module (20) includes a fixed seat (23), a guide bar (242) and a tray (24). The guide bar (242) is installed on the fixed seat (23), and the tray (24) is detachably installed on the guide bar (242). The cleaning tank (21) and all the glass slide slots (22) are formed on the tray (24).

3. The dyeing module according to claim 2, wherein A guide groove (241) is formed on the guide bar (242), and the tray (24) can enter the guide groove (241) and move along the longitudinal direction of the guide groove (241); A positioning structure is formed on the guide bar (242). During the movement of the tray (24) in the guide groove (241), the positioning structure is located on the movement path of the tray (24) and can be locked with the tray (24) mutually; The glass slide module (20) further includes a position detection member (25). The position detection member (25) is communicatively connected with the robotic arm module (10). When the positioning structure is locked with the tray (24) mutually, the position detection member (25) is triggered.

4. The dyeing module according to claim 2, wherein The staining module further includes a dilution module (30). The dilution module (30) includes a plurality of dilution plates (31), and all the dilution plates (31) are arranged on the fixed seat (23); And / or, a plurality of upright columns (32) are further arranged on the fixed seat (23).

5. The dyeing module according to claim 1, characterized in that The robotic arm module (10) includes a first motion component (12), a second motion component (13) and a third motion component (14). The second motion component (13) is arranged on the first motion component (12) and can move along a first direction (X) under the action of the first motion component (12). The third motion component (14) is arranged on the second motion component (13) and can move along a second direction (Y) under the action of the second motion component (13); The liquid suction and injection needle (11) is arranged on the third motion component (14) and can move along a third direction (Z) under the action of the third motion component (14). The first direction (X), the second direction (Y) and the third direction (Z) intersect pairwise and are not coplanar.

6. The dyeing module according to claim 5, characterized in that, The third motion component (14) includes a driving member (40), a cam (41), a mounting component (42), and a movable component (43). The driving member (40) is configured to drive the cam (41) to rotate. The rotation axis direction of the cam (41) intersects with the third direction (Z). The movable component (43) is movably mounted on the mounting component (42) along the third direction (Z), and the movable component (43) is in contact and cooperation with the outer peripheral surface of the cam (41). The injection needle (11) is located on the movable component (43).

7. The dyeing module according to claim 6, wherein The outer peripheral surface of the cam (41) has a highest point (44) and a lowest point (45). The distance from the highest point (44) to the rotation axis is greater than the distance from the lowest point (45) to the rotation axis. The third motion component (14) further includes an elastic member (46). The elastic member (46) is elastically disposed between the movable component (43) and the guiding member and is deformable along the third direction (Z). When the contact point between the movable component (43) and the cam (41) moves from the lowest point (45) to the highest point (44), the elastic member (46) accumulates elastic force. When the contact point between the movable component (43) and the cam (41) moves from the highest point to the lowest point, the elastic member (46) releases elastic force. The elastic force is used to make the movable component (43) press against the cam (41).

8. The dyeing module according to claim 7, characterized in that, The mounting component (42) includes a mounting plate (421) and a guiding block (423). The guiding block (423) is disposed on the mounting plate (421) and is provided with a guiding hole (424) extending longitudinally along the third direction (Z). The movable component (43) includes a roller (431), a connecting plate (432), and a connecting column (433) arranged in sequence along the third direction (Z). The roller (431) is movably mounted on the mounting plate (421) along the third direction (Z), and the outer peripheral surface of the roller (431) is in contact with the outer peripheral surface of the cam (41). One end of the connecting plate (432) in the third direction (Z) abuts against the roller (431), and the other end is connected to one end of the connecting column (433). The connecting column (433) passes through the guiding hole (424), and the other end is provided with the injection needle (11). The elastic member (46) is sleeved on the connecting column (433), and both ends of the elastic member (46) respectively abut against the connecting plate (432) and the guiding block (423).

9. The dyeing module according to claim 5, characterized in that, The staining module further includes a vision module (33). The vision module (33) is disposed on the third motion component (14) and is configured to identify the glass slide (221) in the glass slide groove (22).

10. An immunofluorescence staining instrument, characterized in that, Comprising the staining module according to any one of claims 1-9.

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