Trigger for inoculating and separating microorganisms in first area of culture medium

By designing an automated scratch trigger, the problem of complex structure and low efficiency of microbial inoculation instruments in the prior art is solved, and efficient and low-cost microbial inoculation separation is achieved to meet the compatibility needs of different petri dishes.

CN223189192UActive Publication Date: 2025-08-05LANGMAI (SHANDONG) BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202422112086.9
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-08-29
Publication Date
2025-08-05
Estimated Expiration
2034-08-29

AI Technical Summary

Technical Problem

In the prior art, the microbial inoculation instrument has complex structure, large size, low efficiency, high cost and poor compatibility with different petri dishes, which cannot meet the differentiated needs of the inspection institutions.

Method used

A scratch trigger including a rack table, a turntable, a Petri dish bin rack, a Petri dish inoculation marking separation device and a Petri dish handling device was designed. Through a simple design of mechanical structure, the automatic handling, identification and inoculation of Petri dishes is realized, with high integration and adapting to the compatibility of different Petri dishes.

Benefits of technology

It realizes microbial inoculation and separation with simple mechanism, high integration, large processing volume, low cost and high efficiency, and meets the diverse needs of inspection institutions.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model relates to the technical field of biological sample pretreatment, in particular to a paddle for inoculating and separating microorganisms in a first area of a culture medium, which comprises a rack table, rotary tables are mounted on two sides of the rack table, a culture dish bin frame is rotatably arranged on each group of rotary tables, untreated culture dishes are placed in one group of culture dish bin frames, and the culture dishes are placed in the other group of culture dish bin frames. The other group is used for placing treated culture dishes; a culture dish inoculation lineation separation device is also arranged between the two groups of rotary tables on the rack table; a culture dish carrying device is further arranged on the rack table, the culture dish carrying device is used for transferring untreated culture dishes taken out from one group of culture dish bin frames to the culture dish inoculation marking and separating device for inoculation and then transferring the untreated culture dishes to the other group of culture dish bin frames, the mechanism is simple, the integration degree is high, the treatment capacity is large, the cost is low, and the efficiency is high.
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Description

Technical Field

[0001] The utility model relates to the technical field of biological sample pretreatment, and specifically refers to a streaking trigger for inoculating and separating microorganisms in the first area of a culture medium. Background Technique

[0002] Generally, inspection institutions use manual streaking inoculation methods or conventional automatic inoculators. Manual operation is greatly affected by human factors and is likely to affect the effect of microorganism inoculation and cultivation. Conventional automatic inoculators have complex structures, large volumes, low efficiency, high costs, poor compatibility with different culture dishes, and cannot meet the differentiated needs of inspection institutions.

[0003] This solution is combined with manual operation, has a simple mechanical structure, a small volume, high compatibility with culture dishes, high processing efficiency, and low costs. Content of the Utility Model

[0004] The utility model aims to at least solve the problems of complex mechanism, low integration, and low efficiency in the prior art.

[0005] This solution provides a streaking trigger for inoculating and separating microorganisms in the first area of a culture medium, which is achieved by the following specific technical means: including a frame table, turntables are installed on both sides of the frame table, and a culture dish bin rack is rotated on each group of turntables. One group of culture dish bin racks is used to place untreated culture dishes, and the other group is used to place treated culture dishes; a culture dish inoculation streaking and separation device is also arranged on the frame table between the two groups of turntables; a culture dish handling device is also arranged on the frame table, and the culture dish handling device is used to transfer the untreated culture dishes taken out from one group of culture dish bin racks to the culture dish inoculation streaking and separation device for inoculation and then transfer them to the other group of culture dish bin racks.

[0006] Preferred Technical Solution 1: Multiple groups of vertical bins are arranged on the periphery of the culture dish bin rack, and multiple groups of culture dishes are stacked in the vertical bins. The side of the vertical bin is open, and at the same time, a pushing channel opening is provided at the bottom wall of the vertical bin;

[0007] A culture dish slideway is arranged at the rear side of the turntable for placing untreated culture dishes close to the culture dish handling device. When the culture dish bin rack for placing untreated culture dishes rotates, it drives multiple groups of pushing channel openings to rotate in sequence to be directly opposite to the culture dish slideway and be in the same plane. The width of the culture dish slideway for placing untreated culture dishes is smaller than the width of the culture dish.

[0008] Preferred Technical Solution 2: A culture dish slideway is also provided near the rear side of the turntable for placing the processed culture dishes, and when the culture dish bin rack for placing the unprocessed culture dishes rotates, multiple pushing channel openings rotate to directly above the front end of the culture dish slideway. The size of the culture dish for placing the processed culture dishes is larger than that of the culture dish, and both the left and right sides and the front side of the top surface of the pushing channel opening are hinged with support plates, and the stacked processed culture dishes are lifted by multiple support plates;

[0009] A horizontal pushing component is provided at the bottom side of the turntable. The horizontal pushing component is used to push the processed culture dishes from the rear end to the front end of the culture dish slideway until they are pushed directly below the pushing channel opening, or to push the unprocessed culture dishes from a set of vertical bins onto the corresponding culture dish slideway for the ejection of the unprocessed culture dishes; the horizontal pushing component includes a horizontal pushing electric screw rod fixed on the machine frame table and located at the bottom side of the turntable. A horizontal pushing plate is fixed on the slider in the horizontal pushing electric screw rod. The horizontal pushing plate is used to push the unprocessed culture dishes from a set of vertical bins onto the corresponding culture dish slideway for the ejection of the unprocessed culture dishes, or to slide the processed culture dishes from the rear end to the front end of the culture dish slideway.

[0010] Preferred Technical Solution 3: An upward pushing component is provided on the machine frame table at the bottom side of the culture dish bin. When the top end of the upward pushing component located below the placement of the unprocessed culture dishes penetrates through the bottom wall of a set of vertical bins close to the culture dish slideway, the stacked culture dishes in the vertical bins are lifted; when the upward pushing component located below the placement of the processed culture dishes extends, the top end pushes the processed culture dishes that have moved directly below the pushing channel opening upward and passes through the pushing channel opening and into the vertical bins; the upward pushing component includes a screw motor fixed on the machine frame table and located below the culture dish bin rack. A screw rod fixed to the output shaft of the screw motor is sleeved on an installation plate through a threaded hole. At the same time, the installation plate is also slidably connected to a sliding rod fixed on the machine frame table. The top ends of the vertical rods on both sides of the installation plate are both fixed with top plates.

[0011] Preferred Technical Solution 4: A limiting component is fixed above the culture dish slideway on the turntable for placing the unprocessed culture dishes. The limiting component extends from the side opening of a set of vertical bins close to the culture dish slideway and laterally presses and limits the second group counted from the bottom lifted by the upward pushing component; the limiting component is fixed on the turntable and is a motor fixing plate above the culture dish slideway. A side pressing motor is fixed on the motor fixing plate. A gear fixed to the output shaft of the side pressing motor meshes with a linkage rack fixed on a rack fixing plate that slides on the motor fixing plate. By the rotation of the side pressing motor, the rack fixing plate slides back and forth through the meshing of the gear teeth, making a movement closer to or farther away from the culture dish bin rack, and realizing the lateral pressing of the culture dishes in the culture dish bin rack.

[0012] Preferred Technical Solution Five: The petri dish handling device includes a rear frame fixed on the machine frame table. An xyz three-axis motion platform is arranged on the rear frame, and an electric gripper is fixed on a slider of the z-axis module in the xyz three-axis motion platform. The electric gripper is used to hold the petri dish, and at the same time, the xyz three-axis motion platform is used to drive the petri dish held by the gripper to be transferred between the turntable and the petri dish inoculation device.

[0013] Preferred Technical Solution Six: A rotation motor is fixed on the bottom side of the turntable. The output shaft of the rotation motor is fixedly connected to the center of the bottom side of the petri dish storage rack, and is used to drive the petri dish storage rack to rotate.

[0014] Preferred Technical Solution Seven: A label detection sensor is further arranged on the slider of the z-axis module. The label detection sensor is used to detect the inoculation label marked on the untreated petri dish.

[0015] Adopting the above structure enables this solution to have the following beneficial effects:

[0016] 1. The mechanism is simple, with high integration, large processing capacity, low cost, and high efficiency;

[0017] 2. The petri dish automatically exits the warehouse. The petri dish handling device transports the petri dish to the detection position, automatically identifies it, and then performs microbial inoculation and streaking. After streaking is completed, the petri dish handling device transports the petri dish to the warehouse entry position for warehousing. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation to the present invention. In the drawings:

[0019] Figure 1 is a schematic diagram of the overall structure of this solution;

[0020] Figure 2 is a schematic diagram of the structure of the limit component of this solution;

[0021] Figure 3 is a schematic diagram of the structure of the petri dish handling device of this solution;

[0022] Figure 4 is a schematic diagram of the structure of the petri dish slideway for placing untreated petri dishes of this solution;

[0023] Figure 5 is a schematic diagram of the structure of the petri dish slideway for placing treated petri dishes of this solution;

[0024] Figure 6 is a schematic diagram of the structure of the horizontal pushing component of this solution;

[0025] Figure 7Schematic structural diagram of the upward pushing component of this solution;

[0026] Figure 8 Schematic structural diagram of the petri dish storage rack for placing unprocessed petri dishes in this solution;

[0027] Figure 9 Schematic structural diagram of the petri dish storage rack for placing processed petri dishes in this solution.

[0028] Among them, 1. Frame table, 2. Turntable, 21. Petri dish slideway, 22. Positioning groove, 3. Petri dish storage rack, 31. Vertical storage position, 32. Pushing channel opening, 33. Tray, 4. Petri dish inoculation scribing and separation device, 5. Petri dish handling device, 51. Rear frame, 52. XZ three-axis movement platform, 53. Electric gripper, 54. Label detection sensor, 6. Horizontal pushing component, 61. Horizontal pushing electric screw rod, 62. Slide block, 63. Horizontal pushing plate, 64. Origin sensor three, 65. Induction piece three, 7. Upward pushing component, 71. Screw rod motor, 72. Mounting plate, 73. Vertical rod, 74. Top plate, 75. Origin sensor one, 76. Induction piece one, 8. Limiting component, 81. Motor fixing plate, 82. Side pressing motor, 83. Gear, 84. Rack fixing plate, 85. Linkage rack, 86. Petri dish roller, 87. Origin sensor two, 88. Induction piece two, 9. Rotating motor. Detailed implementation manners

[0029] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0030] Please refer to Figure 1 , a scribing trigger for inoculating and separating microorganisms in the first area of the culture medium, including a frame table 1;

[0031] Both sides of the frame table 1 are equipped with turntables 2. Each turntable 2 rotates a petri dish storage rack 3. One group of petri dish storage racks 3 is used to place unprocessed petri dishes, and the other group is used to place processed petri dishes. A rotating motor 9 is fixed to the bottom side of the turntable 2, and the output shaft of the rotating motor 9 is fixedly connected to the center of the bottom side of the petri dish storage rack 3 for driving the petri dish storage rack 3 to rotate;

[0032] A petri dish inoculation scribing and separation device 4 is also provided on the frame table 1 between the two turntables 2;

[0033] A culture dish handling device 5 is also provided on the rack table 1. The culture dish handling device 5 is used to transfer the untreated culture dishes taken out from a group of culture dish storage racks 3 to the culture dish inoculation and streaking separation device 4 for inoculation and then transfer them to another group of culture dish storage racks 3.

[0034] Please refer to Figures 1-9 , a streaking machine for inoculating and separating microorganisms in the first area of the culture medium. A plurality of vertical bins 31 are provided on the periphery of the culture dish storage rack 3. The vertical bins 31 are used to stack a plurality of groups of culture dishes. The side of the vertical bin 31 is open. The bottom wall of the vertical bin 31 for placing untreated culture dishes is provided with a pushing channel opening 32. A culture dish slideway 21 is provided on the rear side of the turntable 2 close to the culture dish handling device 5. When the culture dish storage rack 3 rotates, a plurality of pushing channel openings 32 are driven to rotate in sequence to be directly opposite to the culture dish slideway 21 and located in the same plane. The bottom wall of the vertical bin 31 for placing the treated culture dishes is also provided with a pushing channel opening 32. As the culture dish storage rack 3 rotates, the pushing channel openings 32 at the bottoms of the plurality of vertical bins 31 move to the front end above the culture dish slideway 21 in sequence. The size of the pushing channel opening 32 of the vertical bin 31 for placing the treated culture dishes is larger than the size of the culture dish. Both the left and right sides and the front side of the top surface of the pushing channel opening 32 are hinged with a support plate 33. The stacked treated culture dishes are supported by the plurality of support plates 33. When the treated culture dishes move to the front end along the culture dish slideway 21, they are located directly below the pushing channel opening 32.

[0035] A horizontal pushing component 6 is provided at the bottom side of the turntable 2. The horizontal pushing component 6 is used to push the treated culture dishes from the rear end to the front end of the culture dish slideway 21 until they are pushed directly below the pushing channel opening 32, or to push the untreated culture dishes from a group of vertical bins 31 onto the opposite culture dish slideway 21 for pushing out the untreated culture dishes. A positioning groove 22 is provided at the rear end of the culture dish slideway 21 on the turntable 2 for placing the untreated culture dishes, so that the removed untreated culture dishes slide into the positioning groove 22 for positioning and clamping, which is convenient for the electric claw 53 in the culture dish handling device 5 to perform positioning and clamping.

[0036] On the rack table 1, a pushing-up component 7 is arranged at the bottom side of the culture dish bin. When the top end of the pushing-up component 7 located below the unprocessed culture dish penetrates through the bottom wall of a set of vertical bins 31 close to the culture dish slideway 21 and protrudes, it jacks up the stacked culture dishes in the vertical bins 31. When the pushing-up component 7 located below the processed culture dish extends, its top end pushes up the processed culture dish that has moved to directly below the pushing channel opening 32, and the processed culture dish passes through the pushing channel opening 32 and is pushed into the vertical bin 31. During the pushing-up process, the processed culture dish first jacks up the supporting plate 33 to swing upward, expanding the spacing for the processed culture dish to pass through. After the processed culture dish moves above the supporting plate 33, the supporting plate 33 falls back, and then the pushing-up component 7 retracts, so that the processed culture dish can fall back onto the supporting plate 33 again for lifting and bearing.

[0037] On the turntable 2 for placing unprocessed culture dishes, a limiting component 8 is fixed above the culture dish slideway 21. The limiting component 8 extends into the side opening of a set of vertical bins 31 close to the culture dish slideway 21 to laterally press and limit the culture dishes jacked up by the pushing-up component 7. The limiting component 8 corresponding to the culture dish bin rack 3 for placing unprocessed culture dishes is used to laterally press and limit the second group of culture dishes jacked up by the pushing-up component 7 from the bottom side. At this time, the transverse pushing component 6 moves into the pushing channel opening 32. After the pushing-up component 7 retracts, the lowermost unprocessed culture dish falls back to the bottom of the vertical bin 31. At this time, the transverse pushing component 6 pushes this group of unprocessed culture dishes out of the vertical bin 31 along the directly opposite and connected pushing channel opening 32 and culture dish slideway 21 to the end of the culture dish slideway 21, waiting for the culture dish handling device 5 to clamp and transfer them.

[0038] Please refer to Figure 7 , a scribing trigger for inoculating and separating microorganisms in the first area of the culture medium. The pushing-up component 7 includes a screw motor 71 fixed on the rack table 1 and located below the culture dish bin rack 3. A screw fixed to the output shaft of the screw motor 71 is sleeved in an installation plate 72 through a threaded hole. At the same time, the installation plate 72 is also slidably connected to a slide bar fixed on the rack table 1. The top ends of the vertical rods 73 on both sides of the installation plate 72 are both fixed with top plates 74. When the two groups of top plates 74 located below the culture dish bin rack 3 for placing unprocessed culture dishes move upward, they slide through the bottom wall of the culture dish bin rack 3 and are located on both sides of the pushing channel opening 32 to jack up the culture dishes in the vertical bin 31. When the two groups of top plates 74 located below the culture dish bin rack 3 for placing processed culture dishes move upward, they jack up the processed culture dish at the front end of the culture dish slideway 2 and directly below the pushing channel opening 32, and push it up to pass through the pushing channel opening 32 and be pushed into the vertical bin 31.

[0039] An origin sensor 75 is provided on the frame table 1. At the same time, an induction piece 76 is provided on the mounting plate 72. When the mounting plate 72 moves down and resets, the induction piece 76 moves to the position of the origin sensor 75. The origin sensor 75 detects whether the induction piece 76 is reset, so as to detect whether the top plate 74 is reset. The origin sensor 75 is signal-connected to the screw motor 71, and the start and stop states of the screw motor 71 are controlled by the feedback signal.

[0040] Please refer to Figures 5-6 , a scriper for inoculating and separating microorganisms in the first culture medium area. The horizontal pushing component 6 includes a horizontal pushing electric screw rod 61 fixed on the frame table 1 and located at the bottom side of the turntable 2. A horizontal pushing plate 63 is fixed on the slider 62 in the horizontal pushing electric screw rod 61. The horizontal pushing plate 63 slides in the connecting pushing channel opening 32 and the culture dish slideway 21 at the place where the untreated culture dish is placed, so as to push the untreated culture dish from a group of vertical bins 31 onto the corresponding culture dish slideway 21 for pushing out the untreated culture dish, or push the treated culture dish from the rear end to the front end of the culture dish slideway 21 to the lower side of a group of vertical bins 31 for placing the treated culture dish; An origin sensor 64 is fixed on the turntable 2. At the same time, an induction piece 65 is fixed on the slider 62. When the slider 62 resets, the induction piece 65 moves to the position of the origin sensor 64. The origin sensor 64 detects whether the induction piece 65 is reset, so as to detect whether the horizontal pushing plate 63 is reset.

[0041] Please refer to Figure 2 , a scriper for inoculating and separating microorganisms in the first culture medium area. The limiting component 8 includes a motor fixing plate 81 fixed on the turntable 2 and located above the culture dish slideway 21. A side pressing motor 82 is fixed on the motor fixing plate 81. A gear 83 fixed on the output shaft of the side pressing motor 82 meshes with a linkage rack 85 fixed on a rack fixing plate 84 sliding on the motor fixing plate 81. By rotating the side pressing motor 82, the rack fixing plate 84 is driven to slide back and forth by the meshing of the gear teeth, so as to move closer to or away from the culture dish rack 3, and realize the lateral pressing of the culture dish in the culture dish rack 3; Two culture dish rollers 86 are rotatably installed on both sides of the front end of the rack fixing plate 84 close to the culture dish rack 3. When the rack fixing plate 84 moves forward, the two culture dish rollers 86 are used to penetrate into the vertical bin 31 and contact and press against the side wall of the culture dish therein.

[0042] On the motor fixing plate 81, there is a second origin sensor 87. At the same time, on the rack fixing plate 84, there is a second induction piece 88. When the rack fixing plate 84 moves backward and resets, the second induction piece 88 moves to the position of the second origin sensor 87. The second origin sensor 87 detects whether the second induction piece 88 is reset, thereby detecting whether the rack fixing plate 84 is reset. The second origin sensor 87 is signal-connected to the side pressure motor 82, and controls the start and stop state of the side pressure motor 82 through the feedback signal.

[0043] Please refer to Figure 1 and Figure 3 and the streaking trigger for inoculating and separating microorganisms in the first culture medium area. The petri dish handling device 5 includes a rear frame 51 fixed on the frame table 1. On the rear frame 51, there is an xyz three-axis motion platform 52 and an electric gripper 53 fixed on a slider 62 in the z-axis module of the xyz three-axis motion platform 52. The electric gripper 53 is used to hold the petri dish. At the same time, the xyz three-axis motion platform 52 is used to drive the petri dish held by the gripper to be transferred between the turntable 2 and the petri dish inoculation device. At the same time, the slider 62 in the z-axis module is connected to the electric gripper 53 through a rotating motor. The output shaft of the rotating motor drives the electric gripper 53 to rotate, and can twist and open or close the petri dish cover when the electric gripper 53 holds the petri dish cover. On the slider 62 in the z-axis module, there is also a label detection sensor 54, and the label detection sensor 54 is used to detect the inoculated label marked on the untreated petri dish.

[0044] Please refer to Figure 1 and the streaking trigger for inoculating and separating microorganisms in the first culture medium area. A controller is installed on the frame table 1. The controller is connected to the first origin sensor 75, the rotating motor 9, the xyz three-axis motion platform 52, the electric gripper 53, the side pressure motor 82, the horizontal push electric screw rod 61, the screw rod motor 71, the second origin sensor 87, and the third origin sensor 64, and is used to control their working states.

[0045] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A paddling machine for inoculating and separating microorganisms in a culture medium, comprising a machine frame (1), characterized in that: Turntables (2) are installed on both sides of the frame (1), and culture dish racks (3) are rotated on each group of the turntables (2). One group of the culture dish racks (3) is used to place untreated culture dishes, and the other group is used to place treated culture dishes. A culture dish inoculation marking and separation device (4) is also provided on the machine frame (1) between the two sets of turntables (2); The rack (1) is also provided with a culture dish transport device (5), which is used to transfer untreated culture dishes taken out from one group of culture dish racks (3) to the culture dish inoculation and marking separation device (4) for inoculation and then to another group of culture dish racks (3).

2. A scratching machine for inoculating and separating microorganisms in a culture medium according to claim 1, characterized in that: The culture dish rack (3) is provided with a plurality of vertical bins (31) on its circumference. The vertical bins (31) are used to stack a plurality of culture dishes. The sides of the vertical bins (31) are open, and a push channel opening (32) is provided on the bottom wall of the vertical bins (31). The turntable (2) for placing unprocessed culture dishes is provided with a culture dish slideway (21) near the rear side of the culture dish transport device (5). When the culture dish rack (3) for placing unprocessed culture dishes rotates, the plurality of push channel openings (32) are driven to rotate in sequence to face the culture dish slideway (21) and be located in the same plane. The width of the culture dish slideway (21) for placing unprocessed culture dishes is smaller than the width of the culture dishes. The turntable (2) for placing processed culture dishes is also provided with a culture dish slideway (21) near the rear side of the culture dish transport device (5). When the culture dish rack (3) for placing unprocessed culture dishes rotates, multiple groups of push channel openings (32) rotate to the top of the culture dish slideway (21). The size of the culture dish for placing processed culture dishes is larger than that of the culture dish. The top surface of the push channel opening (32) is hingedly connected to the left and right sides and the front side with support plates (33). The multiple groups of support plates (33) are used to support the stacked processed culture dishes. A horizontal pushing assembly (6) is provided on the bottom side of the turntable (2), and the horizontal pushing assembly (6) is used to push the processed culture dishes from the rear end to the front end of the culture dish slide (21) until they are pushed to the bottom of the pushing channel opening (32), or to push the unprocessed culture dishes from a group of vertical bins (31) to the culture dish slide (21) facing the unprocessed culture dishes to push out the unprocessed culture dishes.

3. A paddling machine for inoculating and separating microorganisms in a culture medium according to claim 2, characterized in that: A push-up assembly (7) is provided on the bottom side of the culture dish bin on the rack (1); when the top of the push-up assembly (7) located below the unprocessed culture dishes is extended through the bottom wall of a group of vertical bins (31) near the culture dish slide (21), the culture dishes stacked in the vertical bins (31) are lifted up; when the push-up assembly (7) located below the processed culture dishes is extended, the top of the push-up assembly (7) moves to the position just below the push channel opening (32), and the processed culture dishes are pushed up through the push channel opening (32) and pushed into the vertical bins (31).

4. A paddling machine for inoculating and separating microorganisms in a culture medium according to claim 2, characterized in that: A limiting assembly (8) is fixed on the turntable (2) where the untreated culture dishes are placed, above the culture dish slide (21). The limiting assembly (8) extends from a side opening of a group of vertical bins (31) close to the culture dish slide (21) to perform lateral pressing and limiting on the second group from the bottom side that is lifted up by the push-up assembly (7).

5. A paddling machine for inoculating and separating microorganisms in a culture medium according to claim 3, characterized in that: The push-up assembly (7) comprises a screw motor (71) fixed on the frame (1) and located below the culture dish storage rack (3); a screw fixed to the output shaft of the screw motor (71) is sleeved on a mounting plate (72) through a threaded hole; top plates (74) are fixed to the top ends of the vertical rods (73) on both sides of the mounting plate (72); and the culture dish is pushed into the vertical storage rack (31) from the bottom side through the top plates (74).

6. A paddling machine for inoculating and separating microorganisms in a culture medium according to claim 4, characterized in that: The limiting assembly (8) is fixed on the turntable (2) and is located on a motor fixing plate (81) above the culture dish slide (21); a side pressure motor (82) is fixed on the motor fixing plate (81); a gear (83) fixed on the output shaft of the side pressure motor (82) is engaged with a linkage rack (85) fixed on a rack fixing plate (84) sliding on the motor fixing plate (81).

7. A paddling machine for inoculating and separating microorganisms in a culture medium according to claim 6, characterized in that: Culture dish rollers (86) are rotatably mounted on both sides of the front end of the rack fixing plate (84) near the culture dish rack (3). When the rack fixing plate (84) moves forward, the two groups of culture dish rollers (86) penetrate into the vertical bin (31) to contact and press against the side walls of the culture dishes therein.

8. The paddling machine for inoculating and separating microorganisms in a culture medium according to claim 1, characterized in that: The culture dish transport device (5) comprises a rear frame (51) fixed on a frame platform (1), wherein the rear frame (51) is provided with an xyz three-axis motion platform (52) and an electric clamping claw (53) fixed on a slider (62) in a z-axis module of the xyz three-axis motion platform (52).

9. The paddling machine for inoculating and separating microorganisms in a culture medium according to claim 2, characterized in that: A positioning groove (22) is provided at the rear end of the culture dish slideway (21) on the turntable (2) for placing unprocessed culture dishes.