Multifunctional microorganism culture dish

By designing the sampling components and sealing structure of the multifunctional microbial culture dish, the problem of oxygen pollution during anaerobic microbial sampling process in the prior art is solved, and safe sampling and sample protection are achieved under normal environments.

CN223189196UActive Publication Date: 2025-08-05YANGZHOU UNIV
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Patent Information

Application Number
CN202422772877.4
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-11-14
Publication Date
2025-08-05
Estimated Expiration
2034-11-14

AI Technical Summary

Technical Problem

Existing microbial petri dishes are difficult to effectively isolate oxygen in the air under normal environments, resulting in disruption of activity or death during anaerobic microbial sampling.

Method used

A multifunctional microbial petri dish is designed, including a petri dish body and a sealing plug, and is equipped with a sampling assembly, including a sampling rod, a sealing sleeve, a fixing ring and a sealing cover. Through the design of sliding connections and driving shafts, anaerobic microorganisms in the petri dish are sampled, and a sealing structure is used to prevent oxygen from entering.

Benefits of technology

It realizes safe sampling of anaerobic microorganisms under normal environments, avoids oxygen pollution, and ensures the activity and safe storage of samples.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model relates to the technical field of microorganism culture equipment, and discloses a multifunctional microorganism culture dish, which comprises a main body assembly, a culture dish main body and a sealing plug, and the sealing plug is arranged in a bottleneck of the culture dish main body; the sampling assembly is arranged in the culture dish body and comprises a sampling rod arranged in the culture dish body, a sampling head is fixed to the end of the sampling rod, a positioning ring is fixed to the surface of the sampling rod, a sealing sleeve is slidably connected to the surface of the sampling rod, a fixing ring is fixed to the surface of the sealing sleeve, a sealing cover is fixed to one side of the fixing ring, and a containing groove is formed in the top of a sealing plug. A port of the sealing sleeve is located in the containing groove. According to the culture dish disclosed by the utility model, anaerobic microorganisms in the culture dish main body can be sampled by arranging the sampling assembly, oxygen in air does not need to be worried about entering the culture dish main body, and a taken sample can be protected.
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Description

Technical Field

[0001] The utility model relates to the technical field of microbial culture equipment, in particular to a multifunctional microbial culture dish. Background Art

[0002] In the field of microbiological research and application, the cultivation and sampling of anaerobic microorganisms has always been a challenging task. These microorganisms can only grow and reproduce normally in an oxygen-deficient environment. Once exposed to oxygen in the air, their life activities and metabolic processes may be severely disturbed, and even lead to death.

[0003] Traditional culture dishes are used to culture anaerobic microorganisms, but they are not suitable for situations where sampling is required under normal environmental conditions because they cannot effectively isolate oxygen in the air during the sampling process, thereby failing to ensure the activity of anaerobic microorganisms. Utility Model Content

[0004] The purpose of this section is to summarize some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and in the abstract and title of the utility model to avoid obscuring the purpose of this section, the abstract and the title of the utility model, and such simplifications or omissions shall not be used to limit the scope of the present invention.

[0005] In view of the above problems and / or the problems existing in the existing multifunctional microbial culture dishes, the present utility model is proposed.

[0006] Therefore, the problem to be solved by the present invention is that the existing microbial culture dishes are not convenient for sampling anaerobic microorganisms under normal environmental conditions.

[0007] In order to solve the above technical problems, the present invention provides the following technical solutions: a multifunctional microbial culture dish, comprising:

[0008] A main body component, comprising a culture dish body and a sealing plug, wherein the sealing plug is disposed in the bottleneck of the culture dish body;

[0009] A sampling assembly is arranged in the culture dish body, including a sampling rod arranged in the culture dish body, a sampling head is fixed to the end of the sampling rod, a positioning ring is fixed to the surface of the sampling rod, a sealing sleeve is slidably connected to the surface of the sampling rod, a fixing ring is fixed to the surface of the sealing sleeve, a sealing cover is fixed to one side of the fixing ring, a placement groove is opened on the top of the sealing plug, and the sealing sleeve port is located in the placement groove.

[0010] As a preferred solution of the multifunctional microbial culture dish of the present invention, the sampling assembly further comprises a seal, which is arranged in the sealing plug and comprises a driving shaft sliding in the sealing plug, a connecting rod fixed on the top of the driving shaft, and a handle fixed on the top of the connecting rod.

[0011] As a preferred solution of the multifunctional microbial culture dish of the present invention, the sealing member further comprises a rotating seat sliding on the surface of the drive shaft, a limiting strip is fixed in the rotating seat, a sliding groove is provided on the surface of the drive shaft, and the limiting strip slides in the sliding groove.

[0012] As a preferred solution of the multifunctional microbial culture dish of the present invention, a rocker arm is fixed on one side of the rotating seat, a sealing plate is fixed on the end of the rocker arm, a sampling hole is opened at the bottom of the placement groove, and the sealing plate is used to seal the sampling hole.

[0013] As a preferred solution of the multifunctional microbial culture dish of the present invention, a positioning strip is fixed on one side of the sealing plate, a limiting groove is provided on the surface of the sealing sleeve, and an outlet is provided on one side of the limiting groove.

[0014] As a preferred solution of the multifunctional microbial culture dish of the present invention, a first spring is fixed to one side of the rocker arm, and the other end of the first spring is fixed to the inner wall of the sealing plug.

[0015] As a preferred solution of the multifunctional microbial culture dish described in the present invention, the sampling assembly also includes an isolation piece, which is arranged in the sealing plug and includes a drive ring fixed to the surface of the drive shaft, a second spring is sleeved on the surface of the drive shaft, and an isolation cavity is provided in the sealing plug.

[0016] As a preferred solution of the multifunctional microbial culture dish of the present invention, a support rod is fixed on the surface of the driving shaft, and an insertion rod is fixed on the surface of the support rod.

[0017] As a preferred solution of the multifunctional microbial culture dish of the present invention, an isolation plug is slidably arranged on the surface of the insertion rod, and a third spring is sleeved on the surface of the insertion rod.

[0018] As a preferred solution of the multifunctional microbial culture dish of the present invention, an isolation hole is provided at the bottom of the isolation cavity, and the isolation plug is used to seal the isolation hole.

[0019] The beneficial effects of the utility model are as follows: by arranging the sampling component, the anaerobic microorganisms in the culture dish main body can be sampled without worrying about oxygen in the air entering the culture dish main body, and the taken samples can be protected. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without inventive work. Among them:

[0021] Figure 1 This is a structural diagram of a multifunctional microbial culture dish.

[0022] Figure 2 This is a top view of the sealing plug of a multifunctional microbial culture dish.

[0023] Figure 3 This is a cross-sectional structural diagram of a multifunctional microbial culture dish.

[0024] Figure 4 For multifunctional microbial culture dishes Figure 3 A magnified view of the local structure at point A.

[0025] Figure 5 This is a structural diagram of the sealing sleeve of a multifunctional microbial culture dish.

[0026] Figure 6 For multifunctional microbial culture dishes Figure 5 Enlarged view of the local structure at point B in the middle.

[0027] Figure 7 This is a structural diagram of the connection between the rotating seat and the limiting bar of the multifunctional microbial culture dish.

[0028] Figure: main body assembly, 100; culture dish body, 101; sealing plug, 102; sampling assembly, 200; sampling rod, 201a; sampling head, 201b; positioning ring, 201c; sealing sleeve, 201d; fixing ring, 201e; sealing cover, 201f; placement groove, 102-1; sealing element, 202; driving shaft, 202a; connecting rod, 202b; handle, 202c; rotating seat, 202d; limiting bar, 202e; slide groove , 202a-1; rocker arm, 202f; sealing plate, 202g; sampling hole, 102-2; positioning bar, 202h; limiting groove, 201d-1; outlet, 201d-2; first spring, 202i; isolation member, 203; drive ring, 203a; second spring, 203b; isolation chamber, 102-3; support rod, 203c; insertion rod, 203d; isolation plug, 203e; third spring, 203f; isolation hole, 102-4. DETAILED DESCRIPTION

[0029] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below with reference to the accompanying drawings.

[0030] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0031] Secondly, the term "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in various places throughout this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0032] Example 1

[0033] Reference Figures 1 to 7 , which is the first embodiment of the present invention, provides a multifunctional microbial culture dish. The multifunctional microbial culture dish includes a main body assembly 100, including a culture dish body 101 and a sealing plug 102. The sealing plug 102 is arranged in the bottleneck of the culture dish body 101.

[0034] The sealing plug 102 is used to seal the culture dish body 101 , and the culture dish body 101 is used to culture anaerobic microorganisms.

[0035] The sampling assembly 200 is arranged in the culture dish body 101, including a sampling rod 201a arranged in the culture dish body 101, a sampling head 201b is fixed at the end of the sampling rod 201a, a positioning ring 201c is fixed on the surface of the sampling rod 201a, a sealing sleeve 201d is slidably connected to the surface of the sampling rod 201a, a fixing ring 201e is fixed on the surface of the sealing sleeve 201d, a sealing cover 201f is fixed on one side of the fixing ring 201e, a placement groove 102-1 is opened on the top of the sealing plug 102, and the port of the sealing sleeve 201d is located in the placement groove 102-1.

[0036] By inserting the sampling rod 201a into the culture dish body 101, anaerobic microorganisms are sampled through the sampling head 201b. When the sampling rod 201a is pulled, the sampling head 201b leaves the culture dish body 101. When the sampling head 201b enters the sealing sleeve 201d, the positioning ring 201c will push the inner top wall of the sealing sleeve 201d, so that the sealing sleeve 201d is away from the sealing plug 102. At this time, the sealing cover 201f can seal the opening of the sealing sleeve 201d, and the fixing ring 201e is used to fix the sealing cover 201f.

[0037] Specifically, the sampling assembly 200 further includes a sealing member 202 disposed in the sealing plug 102, including a driving shaft 202a sliding in the sealing plug 102, a connecting rod 202b fixed to the top of the driving shaft 202a, and a handle 202c fixed to the top of the connecting rod 202b.

[0038] By rotating the handle 202c, the connecting rod 202b can drive the driving shaft 202a to rotate, and by pulling the handle 202c, the driving shaft 202a can be driven to move up and down in the sealing plug 102.

[0039] Preferably, the seal 202 further includes a rotating seat 202d sliding on the surface of the drive shaft 202a, a limiting strip 202e is fixed in the rotating seat 202d, a sliding groove 202a-1 is opened on the surface of the drive shaft 202a, and the limiting strip 202e slides in the sliding groove 202a-1.

[0040] By setting the limiting strip 202e, the driving shaft 202a can drive the rotating seat 202d to rotate synchronously. When the driving shaft 202a moves up and down, the limiting strip 202e slides in the sliding groove 202a-1.

[0041] Preferably, a rocker arm 202f is fixed to one side of the rotating seat 202d, a sealing plate 202g is fixed to the end of the rocker arm 202f, a sampling hole 102-2 is opened at the bottom of the placement groove 102-1, and the sealing plate 202g is used to seal the sampling hole 102-2.

[0042] The rocker 202f is used to fix and support the sealing plate 202g. The sealing plate 202g slides in the placement groove 102-1 to seal the sampling hole 102-2.

[0043] Preferably, a positioning strip 202h is fixed to one side of the sealing plate 202g, a limiting groove 201d-1 is provided on the surface of the sealing sleeve 201d, and an outlet 201d-2 is provided on one side of the limiting groove 201d-1.

[0044] The positioning bar 202h is engaged in the limiting groove 201d-1, thereby limiting the sealing sleeve 201d, so that the sealing sleeve 201d will not be easily separated from the sealing plug 102. By rotating the sealing sleeve 201d, the outlet 201d-2 can be moved to the side of the positioning bar 202h. At this time, the sealing sleeve 201d can be pulled to make the positioning bar 202h slide out of the limiting groove 201d-1 from the outlet 201d-2, and the sealing sleeve 201d can be removed.

[0045] Preferably, a first spring 202i is fixed to one side of the rocker arm 202f, and the other end of the first spring 202i is fixed to the inner wall of the sealing plug 102.

[0046] The first spring 202i is in a stretched state. The reset tension of the first spring 202i causes the first spring 202i to pull the rocker 202f, thereby causing the rocker 202f to drive the sealing plate 202g to seal the sampling hole 102-2.

[0047] Example 2

[0048] Reference Figure 4 , which is the second embodiment of the present utility model, and this embodiment is based on the previous embodiment:

[0049] Preferably, the sampling assembly 200 also includes an isolation member 203, which is arranged in the sealing plug 102, including a drive ring 203a fixed to the surface of the drive shaft 202a, a second spring 203b is sleeved on the surface of the drive shaft 202a, and an isolation chamber 102-3 is provided in the sealing plug 102.

[0050] The second spring 203b is in a compressed state. The restoring elastic force of the second spring 203b can squeeze the drive ring 203a, so that the drive ring 203a drives the drive shaft 202a to move downward and reset. The second spring 203b and the drive ring 203a are both located in the isolation cavity 102-3.

[0051] Specifically, a support rod 203c is fixed on the surface of the driving shaft 202a, and an insertion rod 203d is fixed on the surface of the support rod 203c.

[0052] The support rod 203c is used to support the insertion rod 203d, so that when the driving shaft 202a rotates or moves up and down, it can drive the insertion rod 203d to move synchronously.

[0053] Specifically, an isolation plug 203e is slidably arranged on the surface of the insertion rod 203d, and a third spring 203f is sleeved on the surface of the insertion rod 203d.

[0054] The third spring 203f is in a compressed state, and is used to push the isolation plug 203e so that the isolation plug 203e is tightly attached to the inner bottom wall of the isolation cavity 102-3.

[0055] Preferably, an isolation hole 102 - 4 is opened at the bottom of the isolation cavity 102 - 3 , and the isolation plug 203 e is used to seal the isolation hole 102 - 4 .

[0056] The isolation hole 102-4 is used to separate the isolation cavity 102-3 and the culture chamber of the culture dish body 101. The sampling rod 201a can enter the culture chamber of the culture dish body 101 through the isolation hole 102-4. The isolation cavity 102-3 and the sealing sleeve 201d are filled with carbon dioxide gas, thereby preventing oxygen in the air from entering the isolation cavity 102-3 and the sealing sleeve 201d.

[0057] During use, when it is necessary to sample the anaerobic microorganisms in the culture dish body 101, the sealing cover 201f is first opened so that the end of the sealing sleeve 201d is tightly attached to the top of the sealing plate 202g. At this time, the handle 202c can be pulled upward to drive the drive shaft 202a to rise, so that the rod 203d drives the isolation plug 203e away from the isolation hole 102-4. At this time, the handle 202c is moved again to drive the drive shaft 202a to rotate, so that the drive shaft 202a drives the rotating seat 202d to rotate synchronously. The rotating seat 202d drives the sealing plate 202g to move synchronously through the rocker 202f, so that the sealing sleeve 201d port on the top of the sealing plate 202g falls into the placement groove 102-1 and is located at the top of the sampling hole 102-2. At this time, the positioning bar 202h enters the limiting groove 201d-1 through the outlet 201d-2. At this time, the sealing sleeve 201d is rotated so that the positioning bar 202h limits the sealing sleeve 201d, thereby stably connecting the sealing sleeve 201d and the sealing plug 102.

[0058] During the rotation of the drive shaft 202a, the drive shaft 202a drives the isolation plug 203e to move synchronously through the insertion rod 203d, so that the isolation plug 203e is away from the isolation hole 102-4. At this time, the sampling rod 201a can be moved, so that the sampling head 201b enters the culture dish body 101 through the sampling hole 102-2 and the isolation hole 102-4, thereby sampling anaerobic microorganisms and causing the anaerobic microorganisms to adhere to the surface of the sampling head 201b.

[0059] When sampling is completed, the sampling rod 201a is pulled to make the sampling head 201b leave the culture dish body 101. When the sampling head 201b enters the sealing sleeve 201d, the positioning ring 201c will push the inner top wall of the sealing sleeve 201d. At this time, the sealing sleeve 201d is rotated to move the outlet 201d-2 to the side of the positioning bar 202h. At this time, the sealing sleeve 201d can be pulled to make the positioning bar 202h slide out of the limiting groove 201d-1 from the outlet 201d-2. At this time, the sealing sleeve 201d can be removed, and the opening of the sealing sleeve 201d can be sealed by the sealing cover 201f, so that the anaerobic microbial sample is stored in the sealing sleeve 201d filled with carbon dioxide.

[0060] When the sealing sleeve 201d moves out of the placement groove 102-1, under the reset tension of the first spring 202i, the rocker arm 202f can drive the sealing plate 202g to seal the sampling hole 102-2, and at the same time drive the driving shaft 202a to rotate, so that the insertion rod 203d drives the isolation plug 203e to move above the isolation hole 102-4. Under the reset elastic force of the third spring 203f, the isolation plug 203e can seal the isolation hole 102-4.

[0061] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, and all of these should be included in the scope of the claims of the present invention.

Claims

1. A multifunctional microbial culture dish, characterized in that: include, A main body assembly (100) comprises a culture dish main body (101) and a sealing plug (102), wherein the sealing plug (102) is arranged in the bottleneck of the culture dish main body (101); A sampling assembly (200) is arranged in the culture dish body (101), comprising a sampling rod (201a) arranged in the culture dish body (101), a sampling head (201b) being fixed to the end of the sampling rod (201a), a positioning ring (201c) being fixed to the surface of the sampling rod (201a), a sealing sleeve (201d) being slidably connected to the surface of the sampling rod (201a), a fixing ring (201e) being fixed to the surface of the sealing sleeve (201d), a sealing cover (201f) being fixed to one side of the fixing ring (201e), a placement groove (102-1) being provided at the top of the sealing plug (102), and a port of the sealing sleeve (201d) being located in the placement groove (102-1).

2. The multifunctional microbial culture dish according to claim 1, wherein: The sampling assembly (200) further includes a sealing member (202) disposed in the sealing plug (102), including a driving shaft (202a) sliding in the sealing plug (102), a connecting rod (202b) fixed to the top of the driving shaft (202a), and a handle (202c) fixed to the top of the connecting rod (202b).

3. The multifunctional microbial culture dish according to claim 2, wherein: The sealing member (202) further comprises a rotating seat (202d) sliding on the surface of the driving shaft (202a), a limiting strip (202e) being fixed in the rotating seat (202d), a sliding groove (202a-1) being provided on the surface of the driving shaft (202a), and the limiting strip (202e) sliding in the sliding groove (202a-1).

4. The multifunctional microbial culture dish according to claim 3, wherein: A swing rod (202f) is fixed to one side of the rotating seat (202d), a sealing plate (202g) is fixed to the end of the swing rod (202f), a sampling hole (102-2) is opened at the bottom of the placement groove (102-1), and the sealing plate (202g) is used to seal the sampling hole (102-2).

5. The multifunctional microbial culture dish according to claim 4, wherein: A positioning strip (202h) is fixed on one side of the sealing plate (202g), a limiting groove (201d-1) is provided on the surface of the sealing sleeve (201d), and an outlet (201d-2) is provided on one side of the limiting groove (201d-1).

6. The multifunctional microbial culture dish according to claim 5, wherein: A first spring (202i) is fixed to one side of the rocker arm (202f), and the other end of the first spring (202i) is fixed to the inner wall of the sealing plug (102).

7. The multifunctional microbial culture dish according to claim 5 or 6, characterized in that: The sampling assembly (200) further includes an isolation member (203), which is disposed in the sealing plug (102) and includes a drive ring (203a) fixed to the surface of the drive shaft (202a), a second spring (203b) being sleeved on the surface of the drive shaft (202a), and an isolation chamber (102-3) is provided in the sealing plug (102).

8. The multifunctional microbial culture dish according to claim 7, wherein: A support rod (203c) is fixed on the surface of the driving shaft (202a), and an insertion rod (203d) is fixed on the surface of the support rod (203c).

9. The multifunctional microbial culture dish according to claim 8, wherein: An isolation plug (203e) is slidably provided on the surface of the insertion rod (203d), and a third spring (203f) is sleeved on the surface of the insertion rod (203d).

10. The multifunctional microbial culture dish according to claim 9, wherein: An isolation hole (102-4) is provided at the bottom of the isolation cavity (102-3), and the isolation plug (203e) is used to seal the isolation hole (102-4).