Bone collagen separation and extraction device

Abstract

The utility model relates to a bone collagen separation and extraction device. The outer tank is supported by a rack, the bottom of the outer tank is sealed, the top of the outer tank is open, a drain pipe with a drain valve is installed on the bottom wall of the outer tank, and a liquid injection assembly is installed in the middle of the inner wall of the outer tank; a lifting frame capable of lifting and moving is further installed on the top of the machine frame, a window is formed in the middle of the lifting frame, idler wheels are installed on the inner side of the window, a filtering type inner tank with the bottom sealed and the top open is arranged in the window, and the outer wall of the filtering type inner tank makes contact with the idler wheels. When the lifting frame lifts the filtering type inner tank to a high position, the bottom of the filtering type inner tank is separated from the disturbance assembly, and when the lifting frame descends the filtering type inner tank to a low position, the bottom of the filtering type inner tank is combined with the disturbance assembly; the utility model provides a bone collagen protein separation and extraction device, which improves the operation efficiency of separation and extraction, reduces the loss of bone collagen protein and avoids the pollution problem caused by circulation among working procedures.

Description

Technical Field

[0001] The utility model belongs to the technical field of bone tissue processing equipment, and in particular relates to a bone collagen separation and extraction device. Background Art

[0002] Collagen repairs damage to various organs and regulates the lubrication and toughness of joints, allowing for ease of movement and flexibility. Aging and disease are the result of the loss of active collagen, so supplementing collagen is essential. Collagen can rapidly increase bone density, strengthening bones. It also repairs damage to various organs within the body. It can also rapidly eliminate the side effects of radiation and chemotherapy, restoring platelet and white blood cell counts to normal levels.

[0003] One of the methods for extracting collagen is the alkaline cleaning method. The main steps of this method include pre-treating bone tissue (including cleaning and cutting), mixing with buffer to expose the protein, and separating and extracting the protein. It can be seen that the separation and extraction of collagen is a relatively complex process, which requires multiple steps to be well connected. In the prior art, the aforementioned steps are usually carried out in multiple devices, which requires the bone tissue to be transferred between different devices, which will lead to the following problems: first, the efficiency of separation and extraction will be reduced; second, the transfer process between processes will cause the loss of collagen; thirdly, if the transfer between processes is not properly controlled, it will easily lead to pollution problems. In summary, it is necessary to develop and design an integrated collagen separation and extraction facility to solve the aforementioned technical problems. Utility Model Content

[0004] The purpose of the utility model is to provide a collagen separation and extraction device to improve the operational efficiency of separation and extraction, reduce the loss of collagen and avoid contamination problems caused by the flow between processes.

[0005] The technical solution adopted by the utility model is: a collagen protein separation and extraction device, comprising an outer tank supported by a frame, sealed at the bottom and open at the top, a liquid inlet pipe with a liquid inlet valve installed on the top of the side wall of the outer tank, a liquid discharge pipe with a liquid discharge valve installed on the bottom wall of the outer tank, a disturbance component installed in the middle of the bottom wall of the outer tank, a driving component of the disturbance component installed on the frame, and a liquid injection component installed in the middle of the inner wall of the outer tank; a lifting and displacing lifting device is also installed on the top of the frame. A lowering frame is provided with a window in the middle of the lifting frame and rollers are installed on the inner side of the window. A filtering inner tank with a sealed bottom and an open top is provided in the window. The outer wall of the filtering inner tank is in contact with each roller. When the lifting frame lifts the filtering inner tank to a high position, the bottom of the filtering inner tank is separated from the disturbance component. When the lifting frame lowers the filtering inner tank to a low position, the bottom of the filtering inner tank is combined with the disturbance component. It also includes a filtering cylinder with open top and bottom, and a filter cloth is provided on the inner wall of the filtering cylinder.

[0006] Preferably, the disturbance assembly comprises a shaft seat installed in the middle of the bottom wall of the outer tank, a stirring shaft is installed in the shaft seat, and a disturbance impeller is installed on the stirring shaft.

[0007] Preferably, the driving assembly includes a driven pulley installed at the lower end of the stirring shaft and a driving motor installed on the side of the frame. A driving pulley is installed on the output shaft of the driving motor, and the driving pulley and the driven pulley are connected by a transmission belt.

[0008] Preferably, the filtering inner tank has a concave conical bottom, a plug-in sleeve is provided at the center of the conical bottom, and a plug-in shaft section is provided at the upper end of the stirring shaft. When the filtering inner tank is lowered to a low position, the plug-in shaft section enters the plug-in sleeve.

[0009] Preferably, a guide rail is installed on the upper part of the column profile of the frame, and the end of the lifting frame is fixedly connected to the track block located on the guide rail. A fastening threaded hole is also provided on the end profile of the lifting frame, and a locking screw is provided in the fastening threaded hole. The inner end of the locking screw rests on the side of the column profile of the frame.

[0010] Preferably, the liquid injection assembly includes a plurality of support plates whose outer ends are fixedly connected to a mounting plate arranged in the middle of the inner wall of the outer tank, and an annular liquid distribution pipe is fixedly installed on each support plate, and a plurality of nozzles and a liquid inlet interface are installed on the annular liquid distribution pipe. It also includes a liquid supply pipe that passes through the side wall of the outer tank, and the inner end of the liquid supply pipe is docked with the liquid inlet interface.

[0011] Preferably, the outer diameter of the filtration-type cylindrical body is equal to the inner diameter of the filtration-type inner tank.

[0012] The advantages and positive effects of the utility model are:

[0013] The present invention provides a device for separating and extracting collagen. Compared with existing facilities for separating and extracting collagen, the device in the present invention is an integrated facility, that is, the main separation and extraction steps are completed within the device without the need for transferring bone tissue between processes. This not only improves the operational efficiency of separating and extracting collagen, but also solves the problems of collagen loss and collagen contamination caused by the transfer of multiple processes.

[0014] By providing a disturbance component, a disturbance effect can be applied during the cleaning stage of the bone tissue, promoting the dissociation and removal of unnecessary substances on the bone tissue. By providing an injection component, the buffer solution can be injected into the interior in a controllable and dispersed manner. By providing a filtering inner tank that can be combined with and separated from the disturbance component, the bone tissue placed in the filtering inner tank is well soaked, cleaned and disturbed during the cleaning stage, and at the same time, it is driven by rotation by combining with the disturbance component during the separation and extraction stage to produce a centrifugal effect. By providing a filtering cylinder with a filter cloth on the inner side of the filtering inner tank, the buffer solution passes through the filtering cylinder and the filtering inner tank under the support of the centrifugal effect, and the collagen is retained on the filter cloth, thereby achieving efficient separation and extraction of the collagen. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the main structure of the utility model;

[0016] Figure 2 This is a schematic diagram of the main structure of the main part of the utility model;

[0017] Figure 3 yes Figure 2 Schematic diagram of the three-dimensional structure of the liquid injection component.

[0018] In the picture:

[0019] 1. Filter cloth; 2. Roller; 3. Liquid inlet valve; 4. Liquid inlet pipe; 5. Liquid supply pipe; 6. Disturbance impeller; 7. Outer tank; 8. Drain valve; 9. Drain pipe; 10. Frame; 11. Plug-in sleeve; 12. Plug-in shaft section; 13. Shaft seat; 14. Driven pulley; 15. Transmission belt; 16. Filter cylinder; 17. Lifting frame; 18. Locking screw; 19. Guide rail; 20. Liquid injection assembly; 20-1. Support plate; 20-2. Annular liquid distribution pipe; 20-3. Liquid inlet interface; 20-4. Nozzle; 21. Filter inner tank; 22. Drive motor; 23. Support frame; 24. Active pulley. DETAILED DESCRIPTION

[0020] In order to further understand the content, features and effects of the present invention, the following embodiments are given to illustrate in detail.

[0021] See Figure 1 and Figure 2 The collagen protein separation and extraction device of the present invention includes an outer tank 7 supported by a frame 10, with a sealed bottom and an open top. A liquid inlet pipe 4 with a liquid inlet valve 3 is installed on the top of the side wall of the outer tank 7, and a liquid discharge pipe 9 with a liquid discharge valve 8 is installed on the bottom wall of the outer tank 7. A cleaning liquid (a low-temperature, high-concentration sodium hydroxide solution) is injected into the interior of the outer tank 7 through the liquid inlet pipe 4, and the internal mixed liquid is discharged from the outer tank 7 through the liquid discharge pipe 9. The liquid inlet valve 3 and the liquid discharge valve 8 are provided for manually controlling the opening and closing of the liquid inlet pipe 4 and the liquid discharge pipe 9, respectively.

[0022] A disturbance component is also installed in the middle of the bottom wall of the outer tank 7, and a driving component of the disturbance component is installed on the frame 10. The disturbance component driven by the driving component is used to exert a disturbance effect on the internal cleaning liquid during the cleaning stage, which can improve the cleaning effect on the bone tissue and promote the dissociation and removal of some unnecessary substances on the bone tissue.

[0023] In this embodiment, the disturbance component includes a shaft seat 13 installed in the middle of the bottom wall of the outer tank 7, a stirring shaft is installed in the shaft seat 13, and a disturbance impeller 6 is installed on the stirring shaft. Under the driving action provided by the drive component, the disturbance impeller 6 rotates to exert a disturbance effect on the cleaning liquid in the tank.

[0024] In this embodiment, the drive assembly includes a driven pulley 14 mounted at the lower end of the agitator shaft and a drive motor 22 mounted on the side of the frame 10. A driving pulley 24 is mounted on the output shaft of the drive motor 22, and the driving pulley 24 and the driven pulley 14 are connected by a transmission belt 15. As shown in the figure, a support frame 23 is mounted in the middle of the frame 10, and the bottom of the outer tank 7 is fixed to the support frame 23. The support frame 23 has an outwardly extending portion, and a diagonal bracing beam is installed between this extension and the frame 10 to enhance structural strength. The drive motor 22 is mounted on this extension.

[0025] A liquid injection assembly 20 is installed in the middle of the inner wall of the outer tank 7. The liquid injection assembly 20 is used to controllably inject the buffer solution into the tank to avoid direct impact on the bone tissue in the tank. Figure 3 , we can see that:

[0026] The liquid injection assembly 20 comprises multiple support plates 20-1, whose outer ends are fixedly connected to a mounting plate located in the middle of the inner wall of the outer tank 7. An annular liquid dispensing tube 20-2 is fixedly mounted on each support plate 20-1. This annular liquid dispensing tube 20-2 is equipped with multiple nozzles 20-4 and a liquid inlet port 20-3. The assembly also includes a liquid supply tube 5 that extends through the side wall of the outer tank 7, the inner end of which is butt-jointed with the liquid inlet port 20-3. As shown in the figure, the outlet of each nozzle 20-4 faces upward and outward, so that the buffer solution is dissipated when it is sprayed onto the inner wall of the outer tank 7.

[0027] A lifting frame 17 is mounted on top of the frame 10, allowing for movement. A window is located in the center of the lifting frame 17, and rollers 2 are mounted inside the window. A filter-type inner tank 21, sealed at the bottom and open at the top, is located within the window. The outer wall of the filter-type inner tank 21 contacts the rollers 2, which ensure stability during rotation. The lifting frame 17 supports and controls the height of the filter-type inner tank 21, allowing it to transition between a high position and a low position.

[0028] In this embodiment, a guide rail 19 is mounted on the upper portion of the column profile of the rack 10. The end of the lifting frame 17 is fixedly connected to the track block located on the guide rail 19. A threaded fastening hole is also provided on the end profile of the lifting frame 17, and a locking screw 18 is installed in the fastening threaded hole. The inner end of the locking screw 18 abuts the side of the column profile of the rack 10. When the lifting frame 17 is raised or lowered to the desired position, the locking screw 18 is tightened to fix the lifting frame 17 at the current height.

[0029] When the lifting frame 17 lifts the filtration type inner tank 21 to a high position, the bottom of the filtration type inner tank 21 is separated from the disturbance component. In the separated state, the filtration type inner tank 21 is in a stationary state. When the lifting frame 17 lowers the filtration type inner tank 21 to a low position, the bottom of the filtration type inner tank 21 is combined with the disturbance component. In the combined state, the driving action applied by the driving component to the disturbance component is also transmitted to the filtration type inner tank 21, and the filtration type inner tank 21 rotates to produce a centrifugal effect.

[0030] In this embodiment, the filtering inner tank 21 has a concave conical bottom, a plug-in shaft sleeve 11 is provided at the center of the conical bottom, and a plug-in shaft section 12 is provided at the upper end of the stirring shaft. When the filtering inner tank 21 descends to a low position, the plug-in shaft section 12 enters the plug-in shaft sleeve 11, and when the filtering inner tank 21 rises to a high position, the plug-in shaft section 12 is disengaged from the plug-in shaft sleeve 11.

[0031] The pretreated bone tissue is placed inside the filtering inner canister 21. The main body of the filtering inner canister 21 is made of a perforated plate. The pores on the plate should be smaller than the diameter of the pretreated bone tissue to prevent the bone tissue from escaping through the pores on the sidewalls of the filtering inner canister 21. The conical bottom of the filtering inner canister 21 is not provided with pores. Due to its upwardly concave conical bottom, the mixed liquid inside the filtering inner canister 21 can flow outward without accumulating inside.

[0032] The apparatus further comprises a filtering cylinder 16 with an open top and bottom, a filter cloth 1 being provided on the inner wall of the filtering cylinder 16, the outer diameter of the filtering cylinder 16 being equal to the inner diameter of the filtering inner tank 21, and the filtering cylinder 16 being made of a perforated plate. After the bone tissue is soaked in a buffer solution, the filtering cylinder 16 with the filter cloth 1 is installed inside the filtering inner tank 21, that is, the filtering cylinder 16 is superimposed on the inner side of the inner wall of the filtering inner tank 21. The driving motor 22 is started and the drain valve 8 on the drain pipe 9 is opened simultaneously. The mixed liquid then passes through the filter cloth 1 under the action of centrifugation, and the collagen contained in the mixed liquid is retained by the filter cloth 1 and hangs on the filter cloth 1. After the machine is shut down, the filtering cylinder 16 is disassembled and the collagen is collected from the filter cloth 1.

[0033] Working process:

[0034] The bone tissue is pretreated by cutting the fresh bone tissue and removing the cartilage, bone marrow, and surrounding connective tissue, followed by a preliminary water wash. The pretreated bone tissue is placed inside the filtering inner tank 21, which should initially be in a high position. A low-temperature, high-concentration sodium hydroxide solution is injected into the outer tank 7 through the liquid inlet pipe 4. After soaking for a certain period of time, the drive motor 22 is started, and the disturbance impeller 6 forces the cleaning liquid in the tank to flow. This also causes the bone tissue inside the filtering inner tank 21 to be disturbed to a certain extent, promoting the dissociation and removal of some unnecessary substances on the bone tissue.

[0035] Then the driving motor 22 is stopped, and the drain pipe 9 is opened to discharge the internal mixed liquid and residue, and then the drain valve 8 is closed; the buffer solution is then supplied to the injection assembly 20 through the liquid supply pipe 5, and the buffer solution is sprayed onto the inner wall of the outer tank 7 to obtain an energy dissipation effect. The buffer solution level in the outer tank 7 gradually rises until it completely immerses the bone tissue in the filtering inner tank 21; after a certain period of time, the lifting frame 17 is operated to descend to the low position, at this time the plug-in shaft section 12 is combined with the plug-in shaft sleeve 11, and a filtering cylinder 16 with a filter cloth 1 is set inside the filtering inner tank 21, and the drain valve 8 is opened again and Start the drive motor 22, and the internal mixed liquid is gradually discharged through the drain pipe 9. The drive motor 22 drives the filtration type inner tank 21 and the filtration type cylinder 16 to rotate through the disturbance component. Under the centrifugal action, the buffer solution in the filtration type inner tank 21 passes through the filter cloth 1, the filtration type cylinder 16 and the filtration type inner tank 21 in turn. The collagen in the buffer solution is retained by the filter cloth 1 and hangs on the filter cloth 1. When the buffer solution in the tank is completely discharged, the drive motor 22 is stopped, the lifting frame 17 and the filtration type inner tank 21 are raised to a high position, and the filtration type cylinder 16 is removed to collect the collagen from the filter cloth 1.

Claims

1. A collagen separation and extraction device, characterized by: The invention comprises an outer tank (7) supported by a frame (10), having a sealed bottom and an open top, a liquid inlet pipe (4) with a liquid inlet valve (3) installed on the top of the side wall of the outer tank (7), a liquid discharge pipe (9) with a liquid discharge valve (8) installed on the bottom wall of the outer tank (7), a disturbance component installed in the middle of the bottom wall of the outer tank (7), a driving component of the disturbance component installed on the frame (10), and a liquid injection component (20) installed in the middle of the inner wall of the outer tank (7); a lifting frame (17) that can be raised and lowered is also installed on the top of the frame (10), and a window is provided in the middle of the lifting frame (17). The invention relates to a filter type inner tank (21) having a bottom sealed and a top open, and a roller (2) installed on the inner side of the window. The window is provided with a filter type inner tank (21) having a bottom sealed and a top open, and the outer wall of the filter type inner tank (21) contacts the rollers (2). When the lifting frame (17) lifts the filter type inner tank (21) to a high position, the bottom of the filter type inner tank (21) is separated from the disturbance component, and when the lifting frame (17) lowers the filter type inner tank (21) to a low position, the bottom of the filter type inner tank (21) is combined with the disturbance component. The invention also includes a filter type cylinder (16) having both the top and the bottom open, and a filter cloth (1) is provided on the inner wall of the filter type cylinder (16).

2. The collagen separation and extraction device according to claim 1, wherein: The disturbance component comprises a shaft seat (13) installed in the middle of the bottom wall of the outer tank (7), a stirring shaft is installed in the shaft seat (13), and a disturbance impeller (6) is installed on the stirring shaft.

3. The collagen separation and extraction device according to claim 2, wherein: The driving assembly comprises a driven pulley (14) installed at the lower end of the stirring shaft and a driving motor (22) installed at the side of the frame (10); a driving pulley (24) is installed on the output shaft of the driving motor (22); and the driving pulley (24) and the driven pulley (14) are connected by a transmission belt (15).

4. The collagen separation and extraction device according to claim 3, wherein: The filtering inner tank (21) has an upward concave conical bottom, a plug-in shaft sleeve (11) is provided at the center of the conical bottom, and a plug-in shaft section (12) is provided at the upper end of the stirring shaft. When the filtering inner tank (21) is lowered to a low position, the plug-in shaft section (12) enters the plug-in shaft sleeve (11).

5. The collagen separation and extraction device as claimed in claim 4, wherein: A guide rail (19) is installed on the upper part of the column profile of the frame (10), and the end of the lifting frame (17) is fixedly connected to the track block located on the guide rail (19). A fastening threaded hole is also provided on the end profile of the lifting frame (17), and a locking screw (18) is provided in the fastening threaded hole. The inner end of the locking screw (18) is against the side of the column profile of the frame (10).

6. The collagen separation and extraction device according to claim 5, wherein: The liquid injection assembly (20) comprises a plurality of support plates (20-1) whose outer ends are fixedly connected to a mounting plate arranged in the middle of the inner wall of the outer tank (7); an annular liquid distribution pipe (20-2) is fixedly mounted on each support plate (20-1); a plurality of nozzles (20-4) and a liquid inlet interface (20-3) are mounted on the annular liquid distribution pipe (20-2); and a liquid supply pipe (5) penetrating the side wall of the outer tank (7); the inner end of the liquid supply pipe (5) is butt-connected to the liquid inlet interface (20-3).

7. The collagen separation and extraction device according to claim 6, wherein: The outer diameter of the filtering cylinder (16) is equal to the inner diameter of the inner tank (21).

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