Low-temperature device for protein purification

Abstract

The utility model discloses a low-temperature device for protein purification, which comprises a box body, a low-temperature control device body is arranged on the side wall of one side of the box body, a top plate and a plurality of groups of connecting rods are arranged in the box body, the connecting rods are fixedly connected to the bottom of the top plate, and the connecting rods are symmetrically arranged in pairs; by arranging the top plate, the connecting rods and the bottom plate, arranging the placing grooves in the top plate and the bottom plate and arranging the clamping assemblies in the placing grooves, after test tubes for storing protein are inserted into the placing grooves, the outer walls of the test tubes can extrude the clamping plates, so that sliding rods are driven to extrude telescopic springs; at the moment, the telescopic spring is in a compressed state, the resilience force of the telescopic spring is utilized to drive the clamping plate to clamp and position the test tube, and when the top plate and the bottom plate drive the test tube to lift, the test tube is prevented from deviating and even toppling, and the operation stability of the device is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of protein purification, in particular to a low-temperature device for protein purification. Background Art

[0002] Protein separation and purification is widely used in biochemical research applications and is an important operating technique. A typical eukaryotic cell can contain thousands of different proteins, some of which are very abundant and some contain only a few copies. In order to study a specific protein, it must first be purified from other proteins and non-protein molecules. Currently, complete protein purification requires a low-temperature environment, so low-temperature equipment is needed to store protein purification to ensure that the protein can be kept in a low-temperature environment to prevent the high temperature from causing the component proteins to deteriorate and become inactive.

[0003] For example, a new low-temperature device for protein purification (Announcement No.: CN217357617U) includes an outer box, a cooling device and a storage box are provided inside the outer box, the low-temperature environment inside the outer box is maintained by the cooling device, and the protein stored in the storage box is kept in a low-temperature state by adding water into the storage box, the storage box is installed on the cooling device, and a slide groove is provided on the inner wall of the outer box between the storage box and the cooling device, a baffle is detachably provided in the slide groove, and sliders cooperating with the slide groove are provided on both sides of the baffle, and the cooling device is separated from the storage box by the slide groove to prevent the heat generated by the cooling device from affecting the temperature of the storage box, and a plurality of heat dissipation holes are provided, and the heat dissipation holes are provided on the side where the cooling device is installed to facilitate heat dissipation of the cooling device.

[0004] However, the device still has the following defects:

[0005] Although the design of this device takes into account protein stability and purification efficiency in a low-temperature environment, in actual use, the storage tank lacks a clamping mechanism to position the test tubes. When the storage plate is raised or lowered, the test tubes are easily displaced, tilted, or even tipped over due to vibration, which may cause loss and contamination of protein samples. Therefore, we need to propose a low-temperature device for protein purification. Utility Model Content

[0006] The purpose of the utility model is to provide a low-temperature device for protein purification, aiming to solve the problem in the prior art that the storage tank lacks a clamping mechanism for positioning the test tube. When the storage plate is raised or lowered, the test tube is easily displaced, tilted or even toppled due to vibration, which may cause loss and contamination of the protein sample.

[0007] To achieve the above objectives, the present invention provides the following technical solutions:

[0008] A low-temperature device for protein purification comprises a box body, a low-temperature control device body is provided on one side wall of the box body, a top plate is provided inside the box body; and connecting rods fixedly connected to the bottom of the top plate, wherein the connecting rods are provided in a plurality of groups and are symmetrically arranged in pairs; a bottom plate fixedly connected to the bottom ends of the plurality of groups of connecting rods, and placement grooves opened on the top plate and the bottom plate, wherein the placement grooves are provided in a plurality of groups, and two groups of clamping assemblies for positioning test tubes are symmetrically arranged inside the plurality of groups of placement grooves; and a lifting assembly provided inside the box body for adjusting the height of the top plate and the bottom plate.

[0009] Preferably, the clamping assembly includes a cylindrical groove, which is opened on the inner wall of the placement groove. A sliding rod is slidably inserted inside the cylindrical groove, one end of the sliding rod is fixedly connected to a splint, and the other end of the sliding rod is fixedly connected to a telescopic spring.

[0010] Preferably, a V-shaped groove is provided on one side wall of the clamping plate, and one end of the telescopic spring is fixedly connected to one inner wall of the cylindrical groove.

[0011] Preferably, the lifting assembly includes a rotating seat, which is fixedly mounted on an inner wall of one side of the box body, and a ball screw is rotatably mounted on the bottom of the rotating seat, and the bottom end of the ball screw is rotatably mounted on the inner bottom of the box body, and the bottom end of the ball screw passes through the box body and is connected to a drive motor, and a drive block is threadedly connected to the outer wall of the ball screw, and the drive block is fixedly connected to a side wall of one side of the base plate.

[0012] Preferably, the drive motor is fixedly mounted on the bottom of the box, and one end of the output shaft of the drive motor is fixedly connected to the bottom end of the ball screw.

[0013] Preferably, a supporting plate is fixedly mounted on the bottom of the base plate, and a rubber plate is fixedly bonded to the inner bottom of the supporting plate.

[0014] Preferably, a cover plate is hingedly connected to the top of the box body through a hinge, and a lock is provided on the top of the cover plate.

[0015] Compared with the prior art, the beneficial effects of the present invention are:

[0016] The utility model is provided with a top plate, a connecting rod and a bottom plate, and a placement groove is opened on the top plate and the bottom plate. A clamping assembly is also provided inside the placement groove. When a test tube storing protein is inserted into the placement groove, the outer wall of the test tube will squeeze the clamping plate, thereby driving the sliding rod to squeeze the telescopic spring. At this time, the telescopic spring is in a compressed state, and the rebound force of the telescopic spring is used to drive the clamping plate to clamp and position the test tube. When the top plate and the bottom plate drive the test tube to rise and fall, the test tube is prevented from deflecting or even tipping over, which is beneficial to improving the operating stability of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a structural diagram of the utility model;

[0018] Figure 2 This is a schematic structural diagram of the shaft side of the utility model;

[0019] Figure 3 This is a structural diagram of the top plate, bottom plate and lifting assembly of the utility model;

[0020] Figure 4 This is a schematic structural diagram of the clamping assembly of the present utility model.

[0021] In the figure: 1. Box body; 2. Low-temperature control device body; 3. Top plate; 4. Connecting rod; 5. Bottom plate; 6. Placement groove; 7. Clamping assembly; 701. Cylindrical groove; 702. Sliding rod; 703. Clamping plate; 704. Telescopic spring; 8. Lifting assembly; 801. Rotating seat; 802. Ball screw; 803. Drive motor; 804. Drive block; 9. V-groove; 10. Support plate; 11. Rubber plate; 12. Cover plate. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0023] See also Figure 1-4 , the utility model provides a technical solution:

[0024] A low-temperature device for protein purification comprises a box body 1, a low-temperature control device body 2 is arranged on one side wall of the box body 1, the low-temperature control device body 2 comprises a refrigeration unit, a temperature sensor and a temperature controller to ensure the stability and accuracy of the operating temperature, a top plate 3 is arranged inside the box body 1, the bottom of the top plate 3 is symmetrically fixedly connected with a plurality of groups of connecting rods 4, the bottom ends of the plurality of groups of connecting rods 4 are fixedly connected with a bottom plate 5, a plurality of groups of placement slots 6 are opened on the bottom plate 5 and the top plate 3, two groups of clamping assemblies 7 for positioning the test tubes are symmetrically arranged inside the plurality of groups of placement slots 6, a lifting assembly 8 for adjusting the height of the top plate 3 and the bottom plate 5 is arranged inside the box body 1, the utility model can clamp and position the test tube by arranging the clamping assembly 7 inside the placement slot 6, and when the top plate 3 and the bottom plate 5 drive the test tube to be lifted and lowered, the test tube is prevented from being offset or even tipping over, which is conducive to improving the operation stability of the device;

[0025] The clamping assembly 7 includes a cylindrical groove 701, which is opened on the inner wall of the placement groove 6. A sliding rod 702 is slidably inserted into the cylindrical groove 701. One end of the sliding rod 702 is fixedly connected to a clamping plate 703, and the other end of the sliding rod 702 is fixedly connected to a telescopic spring 704.

[0026] Specifically, when the test tube containing protein is inserted into the placement slot 6, the outer wall of the test tube presses the clamping plate 703, thereby driving the sliding rod 702 to press the telescopic spring 704. At this time, the telescopic spring 704 is in a compressed state, and the rebound force of the telescopic spring 704 drives the clamping plate to clamp the test tube in place.

[0027] A V-shaped groove 9 is formed on one side wall of the clamping plate 703, and one end of the telescopic spring 704 is fixedly connected to the inner wall of one side of the cylindrical groove 701;

[0028] It is worth mentioning that, since test tubes may have different sizes, the opening of the V-shaped groove 9 can accommodate test tubes of different diameters, and the test tubes are firmly fixed in the V-shaped groove 9 by the clamping force of the clamping plate 703. This design increases the versatility and flexibility of the device, making it applicable to test tubes of various specifications.

[0029] The lifting assembly 8 includes a rotating base 801, which is fixedly mounted on the inner wall of one side of the box body 1. A ball screw 802 is rotatably mounted on the bottom of the rotating base 801. The bottom end of the ball screw 802 is rotatably mounted on the inner bottom of the box body 1, and the bottom end of the ball screw 802 passes through the box body 1 and is connected to a drive motor 803. A drive block 804 is threadedly connected to the outer wall of the ball screw 802, and the drive block 804 is fixedly connected to one side wall of the bottom plate 5.

[0030] Specifically, the output shaft of the drive motor 803 drives the ball screw 802 to rotate in the forward and reverse directions, thereby driving the drive block 804 to reciprocate along the axial direction of the ball screw 802, thereby raising and lowering the top plate 3 and the bottom plate 5, thereby achieving the effect of adjusting the height of the test tube. When the staff needs to take the test tube, the bottom plate 5 and the top plate 3 can be raised first, and the staff does not need to take the test tube by hand inside the box 1. In the long run, low temperature frostbite of the hands can be avoided.

[0031] The drive motor 803 is fixedly mounted on the bottom of the box 1, and one end of the output shaft of the drive motor 803 is fixedly connected to the bottom end of the ball screw 802. The drive motor 803 is configured as a forward and reverse stepping motor;

[0032] A supporting plate 10 is fixedly mounted on the bottom of the bottom plate 5, and a rubber plate 11 is fixedly bonded to the inner bottom of the supporting plate 10. The bottom of the test tube is located on the top of the rubber plate 11, which plays a role in protecting the test tube.

[0033] A cover plate 12 is hinged to the top of the box body 1 through a hinge, and a lock is provided on the top of the cover plate 12.

[0034] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A low temperature device for protein purification, characterized in that: include: A box body (1), a low-temperature control device body (2) is provided on one side wall of the box body (1), and a top plate (3) is provided inside the box body (1); and Connecting rods (4) fixedly connected to the bottom of the top plate (3), wherein the connecting rods (4) are provided in a plurality of groups and are symmetrically arranged in pairs; a bottom plate (5) fixedly connected to the bottom ends of the plurality of groups of connecting rods (4), and Placement slots (6) are provided on the top plate (3) and the bottom plate (5), and the placement slots (6) are provided in a plurality of groups, and two groups of clamping assemblies (7) for positioning the test tubes are symmetrically provided inside the plurality of groups of placement slots (6); A lifting assembly (8) is arranged inside the box (1) and is used to adjust the height of the top plate (3) and the bottom plate (5).

2. A low temperature device for protein purification according to claim 1, characterized in that: The clamping assembly (7) includes a cylindrical groove (701), which is opened on the inner wall of the placement groove (6), and a sliding rod (702) is slidably inserted into the interior of the cylindrical groove (701), one end of the sliding rod (702) is fixedly connected to a clamping plate (703), and the other end of the sliding rod (702) is fixedly connected to a telescopic spring (704).

3. A low temperature device for protein purification according to claim 2, characterized in that: A V-shaped groove (9) is provided on one side wall of the clamping plate (703), and one end of the telescopic spring (704) is fixedly connected to an inner wall of one side of the cylindrical groove (701).

4. A low temperature device for protein purification according to claim 1, characterized in that: The lifting assembly (8) comprises a rotating seat (801), the rotating seat (801) is fixedly mounted on the inner wall of one side of the box body (1), a ball screw (802) is rotatably mounted on the bottom of the rotating seat (801), the bottom end of the ball screw (802) is rotatably mounted on the inner bottom of the box body (1), and the bottom end of the ball screw (802) passes through the box body (1) and is connected to a drive motor (803), a drive block (804) is threadedly connected on the outer wall of the ball screw (802), and the drive block (804) is fixedly connected to the side wall of one side of the bottom plate (5).

5. A low temperature device for protein purification according to claim 4, characterized in that: The driving motor (803) is fixedly mounted on the bottom of the box (1), and one end of the output shaft of the driving motor (803) is fixedly connected to the bottom end of the ball screw (802).

6. A low temperature device for protein purification according to claim 1, characterized in that: A supporting plate (10) is fixedly mounted on the bottom of the base plate (5), and a rubber plate (11) is fixedly bonded to the inner bottom of the supporting plate (10).

7. The low-temperature device for protein purification according to claim 1, characterized in that: The top of the box body (1) is hinged with a cover plate (12) via a hinge, and a lock is provided on the top of the cover plate (12).

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