Multilayer cell culture shaking table

Abstract

The utility model discloses a multilayer cell culture shaking table which comprises a main body mechanism and a constant temperature mechanism, the main body mechanism comprises a box body, a sealing cover hinged to the box body, guide rails symmetrically fixed to the inner wall of the box body, a placing frame arranged in an inner cavity of the box body, an extension plate with one end fixed to the end of the placing frame and the other end extending out of the box body, a motor installed on one side of the box body, and a circular base fixed to a motor shaft. The box body is used for installing other assemblies, the sealing cover is used for sealing the box body to prevent external dust, impurities and the like from entering an inner cavity of the box body, the guide rail is used for installing the placing frame and limiting movement of the placing frame, and the eccentric protrusion is installed on the adjusting piece. The multi-layer cell culture shaking table has the advantages that the amplitude can be adjusted, and the constant-temperature effect is good.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture shakers, in particular to a multi-layer cell culture shaker. Background Art

[0002] The biological culture shaker has stainless steel universal clamps, digital temperature control, stepless speed regulation and good thermal cycle function. It is a multi-purpose biochemical instrument. It is an indispensable laboratory equipment for precision culture preparation in scientific research, education and production departments such as plants, biology, microorganisms, genetics, viruses, environmental protection, and medicine. It is suitable for oscillating culture of various liquid and solid compounds such as biology, biochemistry, cells, and bacteria in major universities and colleges, petrochemical industry, health and epidemic prevention, environmental monitoring and other scientific research departments.

[0003] The existing cell culture shakers have the following main drawbacks during use: the amplitude cannot be adjusted, and it is difficult to adjust the shaker amplitude according to the actual situation of the cells. At the same time, the constant temperature effect of the existing shakers is poor and the heat preservation is uneven. Therefore, there is room for improvement. Utility Model Content

[0004] The utility model aims to solve one of the technical problems existing in the prior art or related technologies.

[0005] To this end, the technical solution adopted by the present invention is: a multi-layer cell culture shaker, comprising: a main body mechanism and a constant temperature mechanism, the main body mechanism comprising a box body, a sealing cover hingedly mounted on the box body, guide rails symmetrically fixed on the inner wall of the box body, a placement rack arranged in the inner cavity of the box body and slidingly engaged on the guide rails at both ends, an extension plate fixed at one end to the end of the placement rack and the other end extending out of the box body, a motor mounted on one side of the box body, a circular seat fixed on the motor shaft, an adjusting member mounted in the inner cavity of the circular seat and extending out of the circular seat, and an eccentric protrusion mounted on the adjusting member.

[0006] The constant temperature mechanism includes an insulation frame fixed on the inner wall of the box, a temperature sensor installed on the inner bottom wall of the insulation frame, a pump installed at the bottom end of the insulation frame and connected to the inner cavity of the insulation frame, and a heating component installed at the bottom end of the insulation frame with one end connected to the pump and the other end extending into the inner cavity of the insulation frame.

[0007] In a preferred example, the present invention can be further configured as follows: the placement rack includes a rectangular plate arranged in the inner cavity of the box and slidingly engaged on the guide rails at both ends, and a bracket fixed in an array at the bottom end of the rectangular plate, and the bracket extends into the insulation frame.

[0008] In a preferred example, the present invention can be further configured as follows: a groove is formed at the top of the circular seat, and the adjusting member includes a threaded rod rotatably mounted on the inner wall of the groove and a knob arranged on one side of the circular seat and with its end fixedly connected to the end of the threaded rod.

[0009] In a preferred example, the present invention can be further configured as follows: the eccentric protrusion includes a threaded sleeve embedded in the groove and a cylinder fixed on the top of the threaded sleeve, the threaded rod passes through the threaded sleeve, and the threaded rod and the threaded sleeve are engaged with each other.

[0010] In a preferred example, the present invention can be further configured as follows: a waist-shaped hole is opened at the end of the extension plate, and the cylinder is embedded in the waist-shaped hole.

[0011] In a preferred example, the present invention can be further configured as follows: the heating component includes a shell fixed to the bottom end of the insulation frame, a connecting pipe arranged through the shell and with one end connected to the pump outlet and the other end extending into the inner cavity of the insulation frame, and an electric heating tube installed in an array in the inner cavity of the shell and sleeved on the outside of the connecting pipe.

[0012] In a preferred example, the present invention can be further configured as follows: the inlet of the pump is connected to the inner cavity of the heat-insulating frame through a water inlet pipe.

[0013] By adopting the above technical solution, the beneficial effects achieved by the utility model are as follows:

[0014] 1. In the utility model, guide rails are installed on the opposite inner walls of the box body, and a placement rack is slidably arranged on the guide rails for placing cell culture tubes. At the same time, an extension plate extending out of the box body is provided on one side of the placement rack, and a waist-shaped hole is opened on the extension plate. A motor is installed on one side of the box body, and a circular seat is fixed on the motor shaft. The circular seat is provided with an eccentric protrusion, and the eccentric protrusion is embedded in the waist-shaped hole. When the motor is started, the circular seat is driven to rotate, and the rotation of the circular seat drives the eccentric protrusion to rotate. The rotation of the eccentric protrusion drives the extension plate to reciprocate, that is, drives the placement rack to reciprocate, and vibrates the cell culture tube. At the same time, an adjusting member is provided on the circular seat, and the distance between the eccentric protrusion and the center of the circular seat can be adjusted by the adjusting member, that is, the position of the eccentric protrusion is adjusted. The closer the eccentric protrusion is to the center of the circular seat, the shorter the reciprocating distance of the extension plate, that is, the smaller the amplitude of the placement rack. Conversely, the amplitude of the placement rack can be adjusted according to the actual situation of the cells, thereby increasing practical performance.

[0015] 2. In the present invention, an insulation frame is installed on the inner wall of the box, and the insulation frame is filled with water. When the cell culture tube is placed on the placement rack, the bottom can be immersed in water. The water bath ensures that the cell culture tube is in a constant temperature environment. At the same time, a temperature sensor is installed on the inner wall of the insulation frame, and a pump and a heating component are installed at the bottom end of the insulation frame. When in use, the pump pumps the water in the insulation frame into the heating component, and the heating component heats the water and then sends it into the insulation box to form a circulation. At the same time, the temperature sensor monitors the temperature of the water in the insulation box in real time, and turns off the pump when the water temperature reaches the set value, ensuring that the water temperature in the insulation frame is stable, further improving practical performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a structural diagram of the utility model;

[0017] Figure 2 This is a schematic diagram of the structure of the utility model after removing the sealing cover;

[0018] Figure 3 It is a cross-sectional schematic diagram of the utility model;

[0019] Figure 4 It is a partial structural diagram of the utility model;

[0020] Figure 5 This is a schematic diagram of the constant temperature mechanism structure of the present utility model.

[0021] Reference numerals:

[0022] 100, main body; 110, housing; 120, sealing cover; 130, guide rail; 140, rack; 141, rectangular plate; 142, bracket; 150, extension plate; 151, waist-shaped hole; 160, motor; 170, circular seat; 171, groove; 180, adjustment member; 181, threaded rod; 182, knob; 190, eccentric protrusion; 191, threaded sleeve; 192, cylinder;

[0023] 200, constant temperature mechanism; 210, insulation frame; 220, temperature sensor; 230, pump; 231, water inlet pipe; 240, heating component; 241, shell; 242, connecting pipe; 243, electric heating pipe. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solution and advantages of the present invention more clear, the present invention is further described in detail below in conjunction with specific embodiments and with reference to the accompanying drawings. It should be noted that the embodiments of the present invention and the features therein can be combined with each other unless there is any conflict.

[0025] Some embodiments of the present invention are described below with reference to the accompanying drawings.

[0026] Example 1:

[0027] Combine Figure 1-5 As shown, this embodiment provides a multi-layer cell culture shaker, including: a main body mechanism 100 and a constant temperature mechanism 200.

[0028] Among them, the main mechanism 100 includes a box body 110, a sealing cover 120 hingedly mounted on the box body 110, a guide rail 130 symmetrically fixed on the inner wall of the box body 110, a placement rack 140 arranged in the inner cavity of the box body 110 and slidingly engaged on the guide rail 130 at both ends, an extension plate with one end fixed to the end of the placement rack 140 and the other end extending out of the box body 110, a motor 160 installed on one side of the box body 110, a circular seat 170 fixed on the shaft of the motor 160, an adjusting member 180 installed in the inner cavity of the circular seat 170 and extending out of the circular seat 170, and an eccentric protrusion 190 installed on the adjusting member 180.

[0029] The box body 110 is used to install other components, the sealing cover 120 is used to seal the box body 110 to prevent external dust and impurities from entering the inner cavity of the box body 110, and the guide rail 130 is used to install the placement rack 140 and limit the movement of the placement rack 140 to ensure the stability of the placement rack 140 during reciprocating movement.

[0030] The placement rack 140 is used to place cell culture tubes, ensure the stability of the cell culture tubes, and drive them to shake. The placement rack 140 includes a rectangular plate 141 set in the inner cavity of the box 110 and slidably engaged on the guide rail 130 at both ends, and a bracket 142 fixed in an array at the bottom end of the rectangular plate 141. The rectangular plate 141 is used to fix the bracket 142, and the bracket 142 is used to support the cell culture tubes.

[0031] One end of the extension plate is fixed on the rectangular plate 141 , and the other end extends out of the box body 110 . The motor 160 is used to install the circular seat 170 and drive the circular seat 170 to rotate.

[0032] A groove 171 is provided at the top of the circular seat 170, and the adjusting member 180 includes a threaded rod 181 rotatably mounted on the inner wall of the groove 171 and a knob 182 provided on one side of the circular seat 170 and fixedly connected to the end of the threaded rod 181. At the same time, the eccentric protrusion 190 includes a threaded sleeve 191 embedded in the groove 171 and a cylinder 192 fixed at the top of the threaded sleeve 191. The threaded rod 181 passes through the threaded sleeve 191, and the threaded rod 181 and the threaded sleeve 191 engage with each other. When the user rotates the threaded rod 181 through the knob 182, the threaded rod 181 rotates to drive the threaded sleeve 191 to move, and the threaded sleeve 191 moves to drive the cylinder 192 to move, thereby adjusting the relative position between the cylinder 192 and the center of the circular seat 170. The closer the cylinder 192 is to the center of the circular seat 170, the shorter the reciprocating distance of the extension plate, that is, the smaller the amplitude of the placement rack 140, and vice versa.

[0033] In addition, a waist-shaped hole 151 is opened at the end of the extension plate 150, and the cylinder 192 is embedded in the waist-shaped hole 151, so that when the motor 160 rotates, it drives the circular seat 170 to rotate, and the rotation of the circular seat 170 drives the eccentric protrusion 190 to rotate. The eccentric protrusion 190 rotates in the waist-shaped hole 151, driving the extension plate 150 to move back and forth, that is, driving the placement rack 140 to move back and forth, thereby vibrating the cell culture tube on the placement rack 140.

[0034] The constant temperature mechanism 200 is used to ensure that the cell culture tube is in a constant temperature environment, including an insulation frame 210 fixed on the inner wall of the box 110, a temperature sensor 220 installed on the inner bottom wall of the insulation frame 210, a pump 230 installed at the bottom end of the insulation frame 210 and connected to the inner cavity of the insulation frame 210, and a heating component 240 installed at the bottom end of the insulation frame 210 with one end connected to the pump 230 and the other end extending into the inner cavity of the insulation frame 210.

[0035] The inner cavity of the heat preservation frame 210 is filled with water, which can keep the water warm. The temperature sensor 220 is used to monitor the temperature of the water in the heat preservation frame 210 in real time.

[0036] The inlet of the pump 230 is connected to the inner cavity of the heat preservation frame 210 through the water inlet pipe 231, and is used to pump water out of the heat preservation frame 210 and send it into the heating component 240.

[0037] The heating assembly 240 is used to heat and increase the temperature of water, and includes a shell 241 fixed to the bottom end of the insulation frame 210, a connecting pipe 242 that is arranged through the shell 241 and one end of which is connected to the outlet of the pump 230 and the other end extends into the inner cavity of the insulation frame 210, and an electric heating pipe 243 that is installed in an array in the inner cavity of the shell 241 and is sleeved on the outside of the connecting pipe 242. When water passes through the connecting pipe 242 under the action of the pump 230, the electric heating pipe 243 is started to heat the water in the connecting pipe 242 to increase its temperature, and then the water is sent back to the insulation frame 210 through the connecting pipe 242 to form a cycle.

[0038] The working principle and use process of the present invention are as follows: when in use, the sealing cover 120 is opened, the cell culture tube is placed in the bracket 142, and the sealing cover 120 is closed. At this time, the bottom of the cell culture tube is immersed in the water in the heat preservation frame 210, the pump 230 is started, the water in the heat preservation frame 210 is extracted and pumped into the connecting pipe 242, and at the same time the electric heating pipe 243 is started to heat the water in the connecting pipe 242 to increase its temperature. The heated water is then sent back into the heat preservation frame 210 through the connecting pipe 242 to form a cycle. At the same time, the temperature sensor 220 monitors the temperature of the water in the heat preservation box 110 in real time, and turns off the pump body after the water temperature reaches the set value, so as to ensure that the water temperature in the heat preservation frame 210 is stable, thereby playing a constant temperature role for the cell culture tube. The amplitude is adjusted according to the actual situation of the cells. The knob 182 is turned to drive the threaded rod 181 to rotate. The rotation of the threaded rod 181 drives the threaded sleeve 191 to move. The movement of the threaded sleeve 191 drives the cylinder 192 to move. The relative position between the cylinder 192 and the center of the circular seat 170 is adjusted. The closer the cylinder 192 is to the center of the circular seat 170, the shorter the reciprocating distance of the extension plate, that is, the smaller the amplitude of the placement rack 140. Conversely, after the adjustment is completed, the motor 160 is started. The rotation of the motor 160 drives the circular seat 170 to rotate. The rotation of the circular seat 170 drives the eccentric protrusion 190 to rotate. The rotation of the eccentric protrusion 190 drives the extension plate 150 to reciprocate, that is, drives the placement rack 140 to reciprocate, thereby realizing the vibration of the cell culture tube on the placement rack 140.

[0039] Although the embodiments of the present invention have been shown and described, those skilled in the art will appreciate that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and purpose of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A multi-layer cell culture shaker, comprising: The main body mechanism (100) and the constant temperature mechanism (200) are characterized in that the main body mechanism (100) includes a box body (110), a sealing cover (120) hingedly mounted on the box body (110), a guide rail (130) symmetrically fixed on the inner wall of the box body (110), a placement rack (140) arranged in the inner cavity of the box body (110) and slidably engaged on the guide rail (130) at both ends, an extension plate with one end fixed to the end of the placement rack (140) and the other end extending out of the box body (110), a motor (160) mounted on one side of the box body (110), a circular seat (170) fixed on the shaft of the motor (160), an adjusting member (180) mounted in the inner cavity of the circular seat (170) and extending out of the circular seat (170), and an eccentric protrusion (190) mounted on the adjusting member (180); The constant temperature mechanism (200) comprises a heat preservation frame (210) fixed on the inner wall of the box (110), a temperature sensor (220) mounted on the inner bottom wall of the heat preservation frame (210), a pump (230) mounted at the bottom end of the heat preservation frame (210) and connected to the inner cavity of the heat preservation frame (210), and a heating component (240) mounted at the bottom end of the heat preservation frame (210) with one end connected to the pump (230) and the other end extending into the inner cavity of the heat preservation frame (210).

2. A multi-layer cell culture shaker according to claim 1, characterized in that: The placement rack (140) includes a rectangular plate (141) disposed in the inner cavity of the box body (110) and slidably engaged on the guide rails (130) at both ends, and brackets (142) fixed in an array at the bottom end of the rectangular plate (141), wherein the brackets (142) extend into the heat-insulating frame (210).

3. The multi-layer cell culture shaker according to claim 1, characterized in that: The top end of the circular seat (170) is provided with a groove (171), and the adjusting member (180) comprises a threaded rod (181) rotatably mounted on the inner wall of the groove (171), and a knob (182) disposed on one side of the circular seat (170) and having an end fixedly connected to the end of the threaded rod (181).

4. The multi-layer cell culture shaker according to claim 3, characterized in that: The eccentric protrusion (190) comprises a threaded sleeve (191) embedded in the groove (171) and a cylinder (192) fixed to the top of the threaded sleeve (191); the threaded rod (181) passes through the threaded sleeve (191), and the threaded rod (181) and the threaded sleeve (191) are engaged with each other.

5. The multi-layer cell culture shaker according to claim 4, characterized in that: A waist-shaped hole (151) is formed at the end of the extension plate (150), and the cylinder (192) is embedded in the waist-shaped hole (151).

6. The multi-layer cell culture shaker according to claim 1, characterized in that: The heating assembly (240) comprises a shell (241) fixed to the bottom end of the heat-insulating frame (210), a connecting pipe (242) passing through the shell (241) and having one end connected to the outlet of the pump (230) and the other end extending into the inner cavity of the heat-insulating frame (210), and electric heating pipes (243) arranged in an array in the inner cavity of the shell (241) and sleeved on the outside of the connecting pipe (242).

7. The multi-layer cell culture shaker according to claim 1, characterized in that: The inlet of the pump (230) is connected to the inner cavity of the heat-insulating frame (210) through a water inlet pipe (231).

Images