Shaking table device for strain cultivation

Abstract

The invention discloses a shaking table device for strain cultivation, and relates to the technical field of microbial devices.The shaking table device comprises a shell, two supporting frames are symmetrically installed in the shell, guide grooves are formed in the tops of the two supporting frames, sliding plates are slidably installed in the two guide grooves, and connecting plates are installed at the tops of the two sliding plates; sliding grooves are formed in the tops of the connecting plates. According to the shaking table device for strain cultivation, through the synergistic effect of the driving piece and the transverse transmission piece, the final bearing platform shaking plate has longitudinal and transverse two-degree-of-freedom movement at the same time. After the two motions are combined, the culture bottle placed on the shaking plate is driven to perform nonlinear composite shaking. The motion mode breaks through the limitation of single reciprocating swing of a traditional shaking table, stronger three-dimensional stirring and eddy current effects can be generated on liquid in a culture bottle, uniform distribution of nutrients, temperature and gas in a container is ensured, and a stable and homogeneous environment is provided for strain growth.

Description

Technical Field

[0001] This invention relates to the field of microbial device technology, and more specifically to a shaker device for bacterial culture. Background Technology

[0002] Microbial culture is a crucial foundational step in fields such as microbial engineering, biopharmaceuticals, food processing, and agricultural technology. In this process, the shaker, as the core equipment for liquid microbial culture, promotes microbial growth and reproduction by providing continuous oscillation. The basic working principle of a shaker is to continuously mix the liquid culture medium within the culture container through mechanical vibration, thereby increasing dissolved oxygen content, maintaining uniform nutrient distribution, and enhancing gas-liquid exchange efficiency, thus creating an optimal growth environment for microorganisms.

[0003] In the process of bacterial culture, the shaking incubator is an important piece of equipment widely used in laboratories and industrial production. By simulating the shaking action in a natural environment, the shaking incubator causes the bacterial culture medium in the culture flask to flow, thereby promoting the uniform distribution of oxygen, nutrients, and metabolic products, and providing the necessary conditions for bacterial growth.

[0004] Currently, most mainstream shaking devices on the market use reciprocating shakers, where the culture flask oscillates linearly up and down or left and right within a single plane. This reciprocating shaking motion makes it difficult to generate a three-dimensional stirring effect on the culture medium, easily creating mixing "dead zones" inside the container, leading to uneven distribution of nutrients, dissolved oxygen, and metabolic products. This is especially true when culturing high-viscosity cultures or high-density bacterial strains, where the mixing differences between the center and edges, and between the upper and lower parts of the liquid are more significant, directly affecting the morphology and metabolic efficiency of mycelial balls. Furthermore, the shaking effect of the shaker is closely related to the dissolved oxygen rate. The shear force generated by the single-dimensional motion on the culture medium is weak, and the gas-liquid interface renewal is slow, failing to fully meet the high oxygen demand of aerobic bacteria during the logarithmic growth phase. Therefore, it becomes a key limiting factor for high-density culture. In summary, current reciprocating shakers, due to their single linear motion mode, uneven shaking, and tendency to form mixing dead zones in the culture medium, resulting in uneven dissolved oxygen, restrict the efficient cultivation of high-density bacterial strains. Summary of the Invention

[0005] The purpose of this invention is to provide a shaking incubator device for bacterial culture, which solves the problem that the existing reciprocating shaking incubator, due to its single linear motion mode, causes uneven shaking, easily forms a mixing dead zone in the culture medium, and leads to uneven dissolved oxygen, thus restricting the efficient cultivation of high-density bacterial strains.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a shaking table device for bacterial culture, comprising a shell, two support frames symmetrically installed inside the shell, a guide groove installed on the top of each of the two support frames, a sliding plate slidably installed inside each of the two guide grooves, a connecting plate installed on the top of each of the two sliding plates, a sliding groove opened on the top of the connecting plate, and a shaking plate slidably connected inside the sliding groove;

[0007] There are two connecting slots. The top of the two connecting slots is connected to the bottom of the two sliding plates respectively. An eccentric wheel is rotatably installed inside each of the two connecting slots. The two eccentric wheels rotate synchronously through a driving component, which is used to drive the sliding plates to move longitudinally inside the guide slot.

[0008] A transverse transmission component, mounted at one end of the eccentric wheel, is used to drive the rocking plate to sway laterally in the sliding groove.

[0009] Furthermore, the transverse transmission component includes two connecting plates rotatably mounted on one end of two eccentric wheels, each connecting plate having a connecting rod at one end. A driven plate is rotatably mounted on the outer surface of the connecting rod, and a shaft is rotatably mounted in the middle of the driven plate, with one end of the shaft mounted inside the housing.

[0010] Furthermore, a connecting shaft is provided at one end of the driven plate, a connecting rod is rotatably mounted on the outer surface of the connecting shaft, a connecting frame is installed on one side of the rocking plate, a crossbar is provided inside the connecting frame, and one end of the connecting rod is rotatably connected to the outer surface of the crossbar.

[0011] Furthermore, the driving component includes a rotating shaft respectively disposed at one end of the two eccentric wheels, and one end of the two rotating shafts respectively passes through the two support frames.

[0012] Furthermore, pulley one is installed on the outer surface of both rotating shafts, and pulley two is installed on one side of both support frames. The pulley one and pulley two are driven by a transmission belt.

[0013] Furthermore, a rotating shaft is rotatably mounted between the two support frames, and both ends of the rotating shaft are respectively connected to one end of the two pulleys.

[0014] Furthermore, a rotating component is installed on one side of the interior of the housing, and one end of the output end of the rotating component is connected to one end of one of the two pulleys.

[0015] Furthermore, it also includes several limiting components, all of which are disposed on the top of the shaking plate and are used to limit the position of the culture bottle.

[0016] Furthermore, the limiting member includes several placement slots formed on the top of the rocking plate.

[0017] Furthermore, each of the aforementioned placement slots is symmetrically provided with two retaining rings, and each of the two retaining rings has a plurality of elastic elements installed on its outer surface, and each of the elastic elements is connected to the inner surface of the placement slot.

[0018] Compared with existing technologies, the shaker device for microbial culture provided by this invention, through the synergistic action of the driving component and the transverse transmission component, enables the final supporting platform, the shaking plate, to simultaneously possess two degrees of freedom of movement: longitudinal and transverse. The combined motion of these two movements drives the culture flasks placed on the shaking plate to undergo nonlinear compound shaking. This motion mode breaks through the traditional single reciprocating oscillation of shakers, generating a stronger three-dimensional stirring and eddy current effect on the liquid inside the culture flasks. This ensures the uniform distribution of nutrients, temperature, and gas within the container, providing a stable and homogeneous environment for microbial growth. Furthermore, traditional linear motion easily creates "dead zones" of slow liquid flow at specific locations within the culture container. The composite shaking trajectory generated by this invention has a wider coverage area and a constantly changing direction of action, effectively impacting every corner of the container. This ensures that the liquid is mixed thoroughly and completely, avoiding the accumulation of localized metabolic waste and nutrient deficiencies, guaranteeing the uniform growth of all bacterial strains, and improving culture quality. Finally, this invention uses a single drive component to drive two eccentric wheels. While achieving the longitudinal movement of the sliding plate, the same eccentric wheel also drives the lateral transmission components, including connecting plates, driven plates, and connecting rods, ultimately converging the movements in both directions onto the shaking plate. This avoids the need for two independent drive systems, making the overall structure more compact and reliable, reducing manufacturing costs and energy consumption, while ensuring the inherent synchronicity and coordination between longitudinal and lateral movements, resulting in highly efficient operation.

[0019] This invention achieves dynamic adaptive clamping of culture flasks through the cooperation of elastic elements and retaining rings. It can automatically adjust the opening distance between the two retaining rings within a certain diameter range to accommodate culture flasks of different sizes. This improves the versatility and ease of operation of the equipment. Furthermore, during the combined motion of the shaker, the clamping force provided by the two retaining rings effectively resists inertial and centrifugal forces in all directions. This mechanism fundamentally prevents the culture flasks from jumping, tipping, or excessively displacing during shaking, ensuring the stability of the culture flasks in the placement tank and thus guaranteeing the safety and reliability of long-term, high-intensity shaking experiments. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0021] Figure 1 This is a schematic diagram of the overall first-view structure provided for an embodiment of the present invention;

[0022] Figure 2 This is a schematic diagram of the internal first-view structure provided in an embodiment of the present invention;

[0023] Figure 3 This is a schematic diagram of the overall second-view structure provided in an embodiment of the present invention;

[0024] Figure 4 Provided for embodiments of the present invention Figure 3 Enlarged view of a portion of point A in the middle;

[0025] Figure 5 This is a schematic diagram of the internal second-view structure provided in an embodiment of the present invention;

[0026] Figure 6 This is a schematic diagram of the internal third-view structure provided for an embodiment of the present invention.

[0027] Explanation of reference numerals in the attached figures:

[0028] 1. Outer shell; 2. Support frame; 3. Guide groove; 4. Sliding plate; 5. Connecting plate; 6. Sliding groove; 7. Rocking plate; 8. Limiting component; 81. Placement groove; 82. Elastic component; 83. Snap ring; 9. Connecting groove; 10. Eccentric wheel; 11. Driving component; 111. Rotating shaft; 112. Belt pulley one; 113. Belt pulley two; 114. Transmission belt; 115. Rotating shaft; 116. Rotating component; 12. Connecting plate; 13. Connecting rod; 14. Driven plate; 15. Shaft; 16. Connecting shaft; 17. Connecting rod; 18. Crossbar; 19. Connecting frame. Detailed Implementation

[0029] To enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0030] As attached Figure 1 To be continued Figure 6 As shown:

[0031] This invention provides a shaking device for bacterial culture, including a shell 1, two support frames 2 symmetrically installed inside the shell 1, a guide groove 3 installed on the top of each of the two support frames 2, a sliding plate 4 slidably installed inside each of the two guide grooves 3, a connecting plate 5 installed on the top of each of the two sliding plates 4, a sliding groove 6 opened on the top of the connecting plate 5, and a shaking plate 7 slidably connected inside the sliding groove 6.

[0032] There are two connecting grooves 9. The top of the two connecting grooves 9 is connected to the bottom of the two sliding plates 4 respectively. An eccentric wheel 10 is rotatably installed inside the two connecting grooves 9. The two eccentric wheels 10 rotate synchronously through the driving component 11, which is used to drive the sliding plate 4 to move longitudinally inside the guide groove 3.

[0033] A transverse transmission component, which is installed at one end of the eccentric wheel 10, is used to drive the rocking plate 7 to sway laterally in the sliding groove 6.

[0034] The transverse transmission component includes two connecting plates 12 that are rotatably mounted on one end of two eccentric wheels 10. Each of the two connecting plates 12 is provided with a connecting rod 13 at one end. A driven plate 14 is rotatably mounted on the outer surface of the connecting rod 13. A shaft 15 is rotatably mounted in the middle of the driven plate 14. One end of the shaft 15 is mounted inside the outer casing 1 on one side.

[0035] A connecting shaft 16 is provided at one end of the driven plate 14. A connecting rod 17 is rotatably mounted on the outer surface of the connecting shaft 16. A connecting frame 19 is installed on one side of the rocking plate 7. A crossbar 18 is provided inside the connecting frame 19. One end of the connecting rod 17 is rotatably connected to the outer surface of the crossbar 18.

[0036] Existing technologies mostly employ simple two-dimensional reciprocating vibration, where the culture bottle oscillates linearly back and forth or left and right within a single plane. This motion mode makes it difficult to produce a complex, three-dimensional stirring effect on the bacterial culture medium inside the culture bottle, resulting in insufficient mixing of the liquid and uneven distribution of nutrients, oxygen, and temperature between the bottom and top, and between the center and the edges of the container, thus affecting the uniformity of bacterial growth and reproductive efficiency.

[0037] In use, the drive unit 11 is first activated, which synchronously drives the two eccentric wheels 10 to rotate within their respective connecting grooves 9. Due to the geometric characteristics of the eccentric wheels 10, their rotational motion is converted into a periodic pushing and pulling action on the sliding plate 4. This causes the sliding plate 4 to move longitudinally up and down along the guide groove 3.

[0038] The two sliding plates 4 are connected as one unit by the connecting plate 5 at the top, thereby driving the entire connecting plate 5 and the rocking plate 7 on it to move longitudinally. This is one degree of freedom of the entire system's motion.

[0039] The rotational motion of the eccentric wheel 10 is further decomposed and transmitted through a lateral transmission component, ultimately generating lateral motion. First, the rotation of the eccentric wheel 10 drives the connected plate 12 and connecting rod 13 to move. The connecting rod 13 pushes the driven plate 14 to swing around a fixed shaft 15. This process converts the rotational motion of the eccentric wheel 10 into a lever-like swing of the driven plate 14. The swinging end of the driven plate 14 drives the connecting rod 17 to move via the connecting shaft 16. The other end of the connecting rod 17 is hinged to a crossbar 18 mounted on the rocking plate 7. Therefore, the swinging motion of the driven plate 14 is converted by the connecting rod 17 into a lateral pushing and pulling force on the rocking plate 7. The rocking plate 7 itself is mounted on the connecting plate 5, which is undergoing longitudinal motion, via a sliding groove 6. Therefore, the rocking plate 7 simultaneously receives two motion inputs: one is longitudinal movement following the connecting plate 5; the other is its own relative motion: lateral movement within the sliding groove 6 driven by the connecting rod 17.

[0040] This invention, through the synergistic action of the driving component 11 and the transverse transmission component, enables the final supporting platform, the shaking plate 7, to possess both longitudinal and transverse degrees of freedom of movement. The combined motion of these two movements drives the culture bottles placed on the shaking plate 7 to undergo nonlinear compound shaking. This motion mode breaks through the traditional single reciprocating oscillation of a shaker, generating a stronger three-dimensional stirring and eddy current effect on the liquid within the culture bottle. This ensures the uniform distribution of nutrients, temperature, and gas within the container, providing a stable and homogeneous environment for bacterial growth. Furthermore, traditional linear motion easily creates "dead zones" of slow liquid flow at specific locations within the culture container. The composite shaking trajectory generated by this invention has a wider coverage area and a constantly changing direction of action, effectively impacting every corner of the container. This ensures that the liquid is mixed thoroughly and without dead angles, avoiding the accumulation of local metabolic waste and nutrient deficiency, guaranteeing the uniform growth of all bacterial strains, and improving the culture quality. Finally, this invention uses a driving component 11 to drive two eccentric wheels 10, achieving longitudinal movement of the sliding plate 4 while simultaneously driving the transverse transmission components (including connecting plate 12, driven plate 14, connecting rod 17, etc.) through the same eccentric wheel 10, ultimately converging the movements in both directions onto the shaking plate 7. This avoids the need for two independent drive systems, making the overall structure more compact and reliable, reducing manufacturing costs and energy consumption, while ensuring the inherent synchronicity and coordination between longitudinal and transverse movements, resulting in highly efficient operation.

[0041] In one embodiment of the present invention, the driving member 11 includes a rotating shaft 111 respectively disposed at one end of two eccentric wheels 10, and one end of the two rotating shafts 111 respectively passes through two support frames 2.

[0042] Both rotating shafts 111 have pulleys 112 mounted on their outer surfaces, and both support frames 2 have pulleys 113 mounted on one side. The pulleys 112 and 113 are driven by a transmission belt 114.

[0043] A rotating shaft 115 is rotatably mounted between the two support frames 2, and the two ends of the rotating shaft 115 are respectively connected to one end of the two pulleys 113.

[0044] A rotating component 116 is installed on one side inside the outer casing 1. One end of the output end of the rotating component 116 is connected to one end of one of the two pulleys 113. The rotating component 116 is a stepper motor.

[0045] Specifically, first, the rotating component 116, which serves as the power source, is activated. Its output shaft begins to rotate, directly driving a pulley 113 connected to it to rotate.

[0046] Since the two pulleys 113 are rigidly connected by a shaft 115, when one pulley 113 is driven by the rotating component 116, power is transmitted to the other pulley 113 via the shaft 115. This ensures that the pulleys 113 on the two support frames 2 rotate completely synchronously (usually in the same direction to maintain consistent motion, depending on the belt winding method). Each pulley 113 transmits power to its corresponding pulley 112 via a transmission belt 114. Each pulley 112 is fixedly mounted on a rotating shaft 111, which is connected to an eccentric wheel 10. Therefore, the rotation of pulley 112 directly drives the rotating shaft 111 and the eccentric wheel 10 to rotate together.

[0047] Ultimately, the two eccentric wheels 10 rotate synchronously under the drive of the drive component 11, which not only ensures the smoothness and reliability of power transmission, but also ensures that the two eccentric wheels 10, the source of the two-way movement acting on the connecting plate 5 and the rocking plate 7, are synchronized. This avoids jamming or internal force loss caused by asynchronous driving, making the compound rocking motion of the entire device smooth, coordinated and efficient.

[0048] In one embodiment of the present invention, a limiting member 8 is also included, which is provided in a plurality of manner. The plurality of limiting members 8 are all disposed on the top of the shaking plate 7, and the plurality of limiting members 8 are all used to limit the position of the culture bottle.

[0049] The limiting member 8 includes several placement slots 81 formed on the top of the rocking plate 7.

[0050] Each of the several placement slots 81 has two retaining rings 83 symmetrically arranged inside. Each of the two retaining rings 83 has several elastic elements 82 installed on its outer surface. Each of the elastic elements 82 is connected to the inner surface of the placement slot 81.

[0051] Specifically, the operator places the culture bottle into the placement slot 81 at the top of the shaking plate 7. The placement slot 81 positions the bottom of the culture bottle, preventing it from moving randomly on the horizontal plane. When the culture bottle is placed into the placement slot 81, its body contacts and compresses two symmetrically arranged retaining rings 83. These retaining rings 83 are connected to the inner wall of the placement slot 81 through several elastic elements 82 installed on their outer surfaces. Under the compression of the culture bottle, the elastic elements 82 (which can be springs, rubber blocks, etc.) cause the two retaining rings 83 to hug the bottle body from both sides. Due to the deformable characteristics of the elastic elements 82, this structure can adapt to culture bottles of different sizes within a certain diameter range, and can provide a stable clamping force.

[0052] When the shaker is started and the shaking plate 7 begins to perform a combined longitudinal and transverse motion, the clamping force formed by the two retaining rings 83 effectively overcomes the inertial force and centrifugal force, preventing the culture flask from jumping out of the placement slot 81, tipping over, or undergoing excessive displacement during shaking, thus ensuring the safety of the experiment.

[0053] Through the cooperation of the elastic element 82 and the retaining ring 83, this structure achieves dynamic adaptive clamping of the culture flask. It can automatically adjust the opening distance between the two retaining rings 83 within a certain diameter range to accommodate culture flasks of different sizes. This improves the versatility and ease of operation of the equipment. Furthermore, during the combined motion of the shaker, the clamping force provided by the two retaining rings 83 effectively resists inertial and centrifugal forces in all directions. This mechanism fundamentally prevents the culture flask from jumping, tipping, or excessively displacing during shaking, ensuring the stability of the culture flask in the placement slot 81, thereby guaranteeing the safety and reliability of long-term, high-intensity shaking experiments.

[0054] The foregoing has only described certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the foregoing drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A shaker device for cultivating a bacterial strain, comprising a housing (1), characterized in that, Two supporting frames (2) are symmetrically arranged inside the shell (1), the top of each of the two supporting frames (2) is provided with a guide groove (3), the inside of each of the two guide grooves (3) is slidably provided with a sliding plate (4), the top of each of the two sliding plates (4) is provided with a connecting plate (5), the top of the connecting plate (5) is provided with a sliding groove (6), the inside of the sliding groove (6) is slidably connected with a rocking plate (7). Two connecting grooves (9) are arranged, the top of each of the two connecting grooves (9) is connected with the bottom of each of the two sliding plates (4), the inside of each of the two connecting grooves (9) is rotatably provided with an eccentric wheel (10), the two eccentric wheels (10) are synchronously rotated through a driving piece (11), which is used for driving the sliding plate (4) to move longitudinally in the guide groove (3). A transverse transmission member is arranged at one end of the eccentric wheel (10), which is used for driving the rocking plate (7) to transversely shake in the sliding groove (6).

2. The shaking device for cultivating bacteria according to claim 1, wherein The transverse transmission member comprises two connecting plates (12) which are rotatably arranged at one end of each of the two eccentric wheels (10), one end of each of the two connecting plates (12) is provided with a connecting rod (13), the outer surface of the connecting rod (13) is rotatably provided with a driven plate (14), the middle of the driven plate (14) is rotatably provided with a shaft rod (15), one end of the shaft rod (15) is arranged inside one side of the shell (1).

3. The shaker device for cultivating bacteria according to claim 2, wherein One end of the driven plate (14) is provided with a connecting shaft (16), the outer surface of the connecting shaft (16) is rotatably provided with a connecting rod (17), one side of the rocking plate (7) is provided with a connecting frame (19), the inside of the connecting frame (19) is provided with a cross rod (18), one end of the connecting rod (17) is rotatably connected with the outer surface of the cross rod (18).

4. The incubator according to claim 1, wherein The driving piece (11) comprises a rotating shaft (111) which is arranged at one end of each of the two eccentric wheels (10), one end of each of the two rotating shafts (111) penetrates through each of the two supporting frames (2).

5. The shaking device for cultivating bacteria according to claim 4, wherein The outer surface of each of the two rotating shafts (111) is provided with a belt pulley one (112), one side of each of the two supporting frames (2) is provided with a belt pulley two (113), the belt pulley one (112) and the belt pulley two (113) are driven through a transmission belt (114).

6. The shaking device for cultivating bacteria according to claim 5, wherein A rotating shaft (115) is rotatably arranged between the two supporting frames (2), the two ends of the rotating shaft (115) are respectively connected with one end of each of the two belt pulley twos (113).

7. The shaking device for cultivating bacteria according to claim 6, wherein One side of the inside of the shell (1) is provided with a rotating piece (116), one end of the output end of the rotating piece (116) is connected with one end of one of the two belt pulley twos (113).

8. The incubator according to claim 1, wherein A plurality of limiting pieces (8) are arranged, each of the plurality of limiting pieces (8) is arranged at the top of the rocking plate (7), and each of the plurality of limiting pieces (8) is used for limiting the strain culture bottle.

9. The shaking device for cultivating bacteria according to claim 8, wherein The limiting piece (8) comprises a plurality of placing grooves (81) which are arranged at the top of the rocking plate (7).

10. The shaking device for cultivating bacteria according to claim 9, wherein Symmetrically, two clamping rings (83) are arranged in each of the placing grooves (81), and the outer surfaces of the two clamping rings (83) are respectively provided with a plurality of elastic members (82), and the elastic members (82) are connected with the inner surfaces of the placing grooves (81).

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