Light path leading-out device of microscopic Raman spectrometer

Abstract

The utility model relates to a light path leading-out device of a microscopic Raman spectrometer, which comprises a first corner reflector accessory, a lens tube, a second corner reflector accessory, an objective lens and a support, the first corner reflector accessory is used for reflecting light beams emitted by the microscopic Raman spectrometer to the lens tube, and the second corner reflector accessory is used for reflecting the light beams emitted by the microscopic Raman spectrometer to the objective lens. The second corner reflector accessory is used for reflecting light beams from the lens tube to the objective lens, the support is used for supporting the lens tube and the objective lens at the same time, and a movable sample table is arranged below the objective lens. The first corner reflector accessory, the lens tube and the second corner reflector accessory are used for secondarily reflecting light beams emitted by the microscopic Raman spectrometer, and the movable sample table is arranged independent of the microscopic Raman spectrometer body, so that the standard movable sample table of the microscopic Raman spectrometer can be adapted to the placement of a large-volume sample.

Description

Technical Field

[0001] The utility model belongs to the field of skin detection and relates to a micro-Raman spectrometer, in particular to a light path extraction device of the micro-Raman spectrometer. Background Art

[0002] The 1930 Nobel Prize in Physics was awarded to the Indian scientist Raman, the discoverer of Raman spectroscopy, and the technique is named after him. Raman discovered that when light interacts with molecules, the wavelength of some light changes (the color changes). By studying this color-shifted scattered light, information about the molecular structure can be obtained.

[0003] In the cosmetics industry, Raman spectroscopy can measure and analyze the content and distribution of moisture and compounds in the skin, layer by layer. This layer-by-layer analysis is essential for studying the mechanisms of human skin. Whether it's transdermal absorption, skin lipid content, or skin lipid ordering, the distribution is layer by layer at different skin depths.

[0004] Due to the characteristics of Raman scanning technology, all current depth imaging is limited to ex vivo skin. In vivo detection only has point testing and cannot complete depth imaging. Specifically, the existing Raman scanning technology uses the classic micro-Raman spectrometer structure to perform deep focus detection on skin samples. There are usually two modes: ex vivo skin, which can perform all-round imaging detection, including planar imaging and depth imaging; living skin, limited parts (such as the back of the hand), single-point depth detection, cannot complete effective imaging. The main reason is that it is limited by the optical path structure and the sample placement method, that is, the depth test at a fixed single position, the test focus is changed by moving the objective lens up and down, and the Raman spectrum of the test position is obtained. The test method is relatively fixed and requires the skin to be close to the test objective lens. This operation will squeeze the skin and cause inaccurate test data. Utility Model Content

[0005] Therefore, the purpose of the present invention is to provide a light path extraction device for a Raman microscope, which can adapt the movable sample stage of the Raman microscope as standard to the placement of large-volume samples and complete Raman imaging testing at the same time.

[0006] In order to solve the problems of the prior art, the utility model provides a light path extraction device for a micro-Raman spectrometer, which mainly comprises a first corner reflector accessory, a lens tube, a second corner reflector accessory, an objective lens, and a bracket. The lens tube is connected to the first corner reflector accessory and the second corner reflector accessory respectively. The first corner reflector accessory is used to reflect the light beam emitted by the micro-Raman spectrometer to the lens tube, and the second corner reflector accessory is used to reflect the light beam from the lens tube to the objective lens. The bracket is used to simultaneously support the lens tube and the objective lens. A movable sample stage is arranged below the objective lens. The movable sample stage is independently arranged from the micro-Raman spectrometer body through the secondary reflection of the light beam emitted by the micro-Raman spectrometer by the first corner reflector accessory, the lens tube, and the second corner reflector accessory. The movable sample stage is connected to a lifting component, and the lifting component is used to lift the movable sample stage.

[0007] Preferably, the first corner reflector attachment and the second corner reflector attachment both comprise a right-angle optical adjustment frame and a reflector arranged on the right-angle optical adjustment frame to reflect the light beam at 90°±10°.

[0008] Preferably, the first end of the first corner reflector accessory is connected to the light emitting part of the Raman microscope, the second end of the first corner reflector accessory is connected to one end of the lens tube, the other end of the lens tube is connected to the first end of the second corner reflector accessory, the second end of the second corner reflector accessory is connected to the objective lens, the central axis of the lens tube is perpendicular to the central axis of the objective lens, and the central axis of the objective lens is parallel to the light emitting direction of the light emitting part.

[0009] Preferably, the bracket includes a horizontally arranged X-axis support rod and a vertically arranged Y-axis support rod, and the ends of the X-axis support rod and the Y-axis support rod are both provided with fixing rings, and the other end of the X-axis support rod is adjustably set on the Y-axis support rod.

[0010] Preferably, an arm fixing mechanism is provided on the upper surface of the movable sample stage, and the arm fixing mechanism includes an arm fixing shell, and the arm fixing shell has an inner cavity and an upward opening, and the opening is used to pass the arm to be detected so as to accommodate the arm to be detected in the inner cavity, and several flexible belts are provided along the longitudinal axis of the arm fixing shell, and the flexible belts are used to fix the arm to be detected in the inner cavity of the arm fixing shell.

[0011] Preferably, the arm fixing shell is a semicircular hard shell.

[0012] Preferably, the arm fixing mechanism includes a fixing plate, through which the arm fixing housing is fixed to the upper surface of the movable sample stage.

[0013] Preferably, the lifting assembly includes a lifting plate, a connecting plate, and a screw lifting mechanism. The lifting plate is horizontally arranged below the movable sample stage. The lifting plate is connected to the screw lifting mechanism through the connecting plate. The screw lifting mechanism drives the lifting of the lifting plate to move up and down to drive the movable sample stage to move up and down.

[0014] Preferably, the screw lifting mechanism is provided with a hand crank or a motor for controlling the screw lifting mechanism.

[0015] The optical path extraction device of the Raman microscope of the present invention can adapt the movable sample stage that comes standard with the Raman microscope to the placement of large-volume samples, and simultaneously complete Raman imaging tests, so that the test mode is not limited to area and position, and single-point testing, XY axis surface testing, YZ axis surface testing, and XYZ axis 3D testing can be performed. Moreover, the testing can be performed non-destructively and contactlessly, avoiding the influence of external factors on the data. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a first structural schematic diagram of the optical path extraction device of the Raman micro-spectrometer of the present utility model.

[0017] Figure 2 This is a second structural schematic diagram of the optical path extraction device of the Raman micro-spectrometer of the present invention.

[0018] Figure 3 This is a third structural schematic diagram of the optical path extraction device of the Raman micro-spectrometer of the present utility model.

[0019] Figure 4 The figure is a structural diagram of the arm fixing mechanism in the optical path extraction device of the micro-Raman spectrometer of the present invention.

[0020] Figure 5 The figure is a structural diagram of the lifting assembly in the optical path extraction device of the Raman micro-spectrometer of the present invention. DETAILED DESCRIPTION

[0021] The present invention will be further described below in conjunction with specific embodiments. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of application of the present invention. The specific implementation methods of the present invention will be described in detail below in conjunction with the technical solutions and accompanying drawings.

[0022] like Figures 1 to 5FIG. 1 is an embodiment of the optical path extraction device of the Raman micro-spectrometer of the present invention. The optical path extraction device 4 is used in conjunction with the Raman micro-spectrometer 3 and is installed on the table 1 to perform various skin tests.

[0023] In the skin penetration test, it is necessary to conduct precise positioning test on the surface of the skin from the stratum corneum to the dermis, including plane imaging from the XY plane dimension and depth imaging from the longitudinal dimension of the Z axis. Conventional micro-Raman spectrometers are equipped with a highly integrated movable sample stage with high-precision stepping for spatial positioning. That is, through the position movement of the high-precision automatic movable sample stage and the confocal characteristics of the Raman spectrometer, the test spot is accurately positioned in three-dimensional space to obtain the Raman data at that position, and then the software is used for analysis and processing to achieve visual imaging. However, the movable sample stage of conventional micro-Raman spectrometers is placed directly under the microscope eyepiece, that is, directly under the microscope eyepiece. Figure 1 On the placement platform 2, the space is small, it is inconvenient to place human body parts, it is almost impossible to perform imaging experiments, and it is not suitable for the placement of large-volume samples.

[0024] The optical path extraction device 4 of the present invention moves the detection light to the outside of the main body of the micro-Raman spectrometer, thus solving the problem of placement on human body parts. Specifically, it includes a first corner reflector accessory 5, a lens tube 6, a second corner reflector accessory 7, an objective lens 11, and a bracket 8. The lens tube 6 is connected to the first corner reflector accessory 5 and the second corner reflector accessory 7 respectively. The first corner reflector accessory 5 is used to reflect the light beam emitted by the micro-Raman spectrometer 3 to the lens tube 6, and the second corner reflector accessory 7 is used to reflect the light beam from the lens tube 6 to the objective lens 11. The bracket 8 is used to support the lens tube 6 and the objective lens 11 at the same time. A movable sample stage 9 is set under the objective lens 11. The movable sample stage is set independently of the micro-Raman spectrometer body through the secondary reflection of the light beam emitted by the micro-Raman spectrometer by the first corner reflector accessory, the lens tube, and the second corner reflector accessory. The movable sample stage 9 is connected to the lifting component 13, and the lifting component 13 is used to lift the movable sample stage 9.

[0025] The optical path extraction device provided by this utility model can adapt the movable sample stage standard on a Raman microscope to accommodate large samples, while also completing Raman imaging tests. Therefore, when using this optical path extraction device for testing, the test mode is not limited to area or position, and single-point testing, XY axis surface testing, YZ axis surface testing, and XYZ axis 3D testing can be performed. Testing is completed through software-automated control of the automatic platform. Furthermore, testing can be performed non-destructively and contactlessly, avoiding the impact of external factors on the data.

[0026] like Figures 1 to 3 As shown, the first corner reflector attachment 5 and the second corner reflector attachment 7 both include a right-angle optical adjustment frame and a reflector arranged on the right-angle optical adjustment frame, reflecting the light beam at 90°±10°, preferably at 90°±2°, and most preferably at 90°. Each corner reflector attachment can be equipped with a conventional device for adjusting the incident angle of the light source with a reflector, which can achieve optical path adjustment at an angle of 90°±10°. That is, through the two-stage reflection principle, the movable sample platform can be separated from the main body of the micro-Raman spectrometer, greatly broadening the scope of application of the test object's shape, size and space. By combining the corner reflector attachment and the lens tube, the light path can be adjusted at any angle and length. Ultimately, through the adjustment of the light path, the white light microscopic observation position is made consistent with the laser test position.

[0027] Specifically, the first end of the first corner reflector attachment 5 is connected to the light output portion 3 of the Raman microscope, the second end of the first corner reflector attachment 5 is connected to one end of the lens tube 6, the other end of the lens tube 6 is connected to the first end of the second corner reflector attachment 7, the second end of the second corner reflector attachment 7 is connected to the objective lens 11, the central axis of the lens tube 6 is perpendicular to the central axis of the objective lens 11, and the central axis of the objective lens 11 is parallel to the light output direction of the light output portion. The central axis of the objective lens 11 can be arranged in a vertical direction, and the central axis of the lens tube 6 can be arranged in a horizontal direction.

[0028] The lens tube 6 used in the present invention can be composed of a plurality of lens tube monomers to adjust the length, and the objective lens includes objective lenses of different magnifications.

[0029] like Figures 1 to 3 As shown, the bracket 8 includes a horizontally arranged X-axis support rod and a vertically arranged Y-axis support rod. Both the X-axis support rod and the Y-axis support rod have fixing rings at their ends, and the other end of the X-axis support rod is adjustably mounted on the Y-axis support rod. By simultaneously fixing the objective lens and the lens tube, the problem of external optical path vibration is resolved.

[0030] like Figures 1 to 4As shown, an arm securing mechanism 10 is provided on the upper surface of the movable sample stage 9. The arm securing mechanism 10 includes an arm securing housing 15 having an inner cavity and an upward opening. The opening is configured to allow the arm to be inspected to pass through the inner cavity and accommodate the arm to be inspected. Several flexible bands are provided along the longitudinal axis of the arm securing housing 15 to secure the arm to be inspected within the inner cavity of the arm securing housing 15. The arm securing housing 15 is a semicircular, rigid housing that can be manufactured using injection molding.

[0031] The arm fixing mechanism includes a fixing plate 14, through which the arm fixing housing 15 is fixed to the upper surface of the movable sample stage. Specifically, the bottom of the inner cavity of the arm fixing housing 15 is provided with mounting holes evenly distributed along the longitudinal axis of the arm fixing housing. These mounting holes are used to install fasteners. Through these mounting holes and the fasteners, the arm fixing housing 15 is fixed to the fixing plate 14. The fixing plate can be made of stainless steel.

[0032] The longitudinal axis of the arm fixing housing 15 is tilted relative to the length direction of the fixing plate 14 .

[0033] Fixing hooks 16 are provided on both sides of the opening of the arm fixing housing 15 for fixing the flexible belt. The fixing hooks 16 are evenly distributed along a longitudinal axis parallel to the arm fixing housing 15.

[0034] The length of the arm fixing shell is 250mm to 350mm, and the diameter is 70 to 90mm. For example, the length is 300mm and the diameter / width is 82mm. The corresponding fixing plate can be about 160mm in length and about 109mm in width.

[0035] The present invention utilizes the arm-fixing mechanism to position and secure the arm, preventing minor vibrations from affecting the imaging quality during Raman imaging. The arm-fixing mechanism utilizes a semi-solid, semi-flexible structure (a semi-circular arc-shaped fixing body as the solid structure and a flexible band as the soft structure) to adjustably secure the human arm, ensuring that experiments are completed with minimal vibration and resulting in excellent imaging results.

[0036] like Figure 5As shown, the lifting assembly includes a lifting plate 12, a connecting plate 19, and a screw lifting mechanism. The lifting plate 12 is horizontally arranged below the movable sample stage 9. The lifting plate 12 is connected to the screw lifting mechanism via the connecting plate 19. The screw lifting mechanism drives the lifting plate 12 up and down to drive the movable sample stage 9 up and down. In the present utility model, the screw lifting mechanism is arranged in the housing 18.

[0037] The screw lifting mechanism is provided with a motor 17 for automatically controlling the screw lifting mechanism, and a hand crank can also be provided to manually control the screw lifting mechanism.

[0038] This utility model uses a lifting platform to achieve millimeter-level Z-axis (lifting) height adjustment of the movable sample stage. The movable sample stage can use the imaging automatic platform provided by the micro-Raman spectrometer. This sample stage generally has a micron stepping function and cannot meet the requirements of large-scale displacement. The lifting platform can effectively assist in height adjustment.

[0039] The optical path extraction device of the Raman microscope of the present invention can adapt the movable sample stage that comes standard with the Raman microscope to the placement of large-volume samples, and simultaneously complete Raman imaging tests, so that the test mode is not limited to area and position, and single-point testing, XY axis surface testing, YZ axis surface testing, and XYZ axis 3D testing can be performed. Moreover, the testing can be performed non-destructively and contactlessly, avoiding the influence of external factors on the data.

[0040] In this specification, the present invention has been described with reference to specific embodiments thereof. However, it will be apparent that various modifications and variations may be made without departing from the spirit and scope of the present invention. Accordingly, the specification and drawings are to be regarded as illustrative rather than restrictive.

Claims

1. A light path extraction device for a micro-Raman spectrometer, characterized in that: The invention comprises a first corner reflector accessory, a lens tube, a second corner reflector accessory, an objective lens, and a bracket. The lens tube is connected to the first corner reflector accessory and the second corner reflector accessory respectively. The first corner reflector accessory is used to reflect the light beam emitted by the micro-Raman spectrometer to the lens tube, and the second corner reflector accessory is used to reflect the light beam from the lens tube to the objective lens. The bracket is used to simultaneously support the lens tube and the objective lens. A movable sample stage is arranged below the objective lens. The movable sample stage is set independently of the micro-Raman spectrometer body through the secondary reflection of the light beam emitted by the micro-Raman spectrometer by the first corner reflector accessory, the lens tube, and the second corner reflector accessory. The movable sample stage is connected to a lifting component, and the lifting component is used to lift the movable sample stage.

2. The light path extraction device of the Raman microscope according to claim 1, characterized in that: The first corner reflector attachment and the second corner reflector attachment both include a right-angle optical adjustment frame and a reflector arranged on the right-angle optical adjustment frame to reflect the light beam at 90°±10°.

3. The light path extraction device of the Raman microscope according to claim 2, characterized in that: The first end of the first corner reflector accessory is connected to the light emitting part of the micro-Raman spectrometer, the second end of the first corner reflector accessory is connected to one end of the lens tube, the other end of the lens tube is connected to the first end of the second corner reflector accessory, the second end of the second corner reflector accessory is connected to the objective lens, the central axis of the lens tube is perpendicular to the central axis of the objective lens, and the central axis of the objective lens is parallel to the light emitting direction of the light emitting part.

4. The light path extraction device for a Raman microscope according to any one of claims 1 to 3, characterized in that: The bracket includes a horizontally arranged X-axis support rod and a vertically arranged Y-axis support rod. Fixed rings are provided at the ends of the X-axis support rod and the Y-axis support rod, and the other end of the X-axis support rod is adjustably arranged on the Y-axis support rod.

5. The light path extraction device for a Raman microscope according to any one of claims 1 to 3, characterized in that: An arm fixing mechanism is provided on the upper surface of the movable sample stage, and the arm fixing mechanism includes an arm fixing shell, and the arm fixing shell has an inner cavity and an upward opening, and the opening is used to pass the arm to be detected so as to accommodate the arm to be detected in the inner cavity, and several flexible belts are provided along the longitudinal axis of the arm fixing shell, and the flexible belts are used to fix the arm to be detected in the inner cavity of the arm fixing shell.

6. The light path extraction device of the Raman microscope according to claim 5, characterized in that: The arm fixing shell is a semicircular arc-shaped hard shell.

7. The light path extraction device of the Raman microscope according to claim 5, characterized in that: The arm fixing mechanism includes a fixing plate, through which the arm fixing housing is fixed to the upper surface of the movable sample stage.

8. The light path extraction device for a Raman microscope according to any one of claims 1 to 3, characterized in that: The lifting assembly includes a lifting plate, a connecting plate, and a screw lifting mechanism. The lifting plate is horizontally arranged below the movable sample stage. The lifting plate is connected to the screw lifting mechanism through the connecting plate. The screw lifting mechanism drives the lifting of the lifting plate to drive the movable sample stage up and down.

9. The light path extraction device of the Raman microscope according to claim 8, characterized in that: The screw rod lifting mechanism is provided with a hand crank or a motor for controlling the screw rod lifting mechanism.

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