Microbioreactor for cell culture

By designing a stirring structure and cleaning mechanism for meshing of bevel gears in the microbioreactor, the problem of insufficient contact between cells and culture medium is solved, and more efficient cell culture and device cleaning is achieved.

CN223214089UActive Publication Date: 2025-08-12ENDOCELL THERAPEUTICS INC
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Patent Information

Application Number
CN202422531512.2
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-10-21
Publication Date
2025-08-12
Estimated Expiration
2034-10-21

AI Technical Summary

Technical Problem

During the stirring process, existing microbioreactors have problems with insufficient contact between cells and culture medium, which leads to a reduction in culture efficiency.

Method used

A microbioreactor is designed to drive the meshing of the tapered gears on the first and third connecting rods through a motor-driven second connecting rod to realize counterclockwise rotation of the stirring paddle, ensure that the cells are in full contact with the nutrient solution, and a cleaning mechanism is equipped to clean up residual impurities on the inner wall.

Benefits of technology

The efficiency of cell culture is improved, the inner wall residue is avoided to affect the next culture, and the practicality and reliability of the device are improved.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model relates to the technical field of cell culture, and discloses a micro bioreactor for cell culture, which comprises a hollow barrel, the output end of a motor penetrates through a barrel cover and is fixedly connected with a second connecting rod, and the middle lower part of the outer side of the second connecting rod is rotatably connected with a hollow column; a third connecting rod is rotatably connected to the bottom of the inner side of the hollow column, first bevel gears are fixedly connected to the adjacent ends of the third connecting rod and the second connecting rod, a second bevel gear is fixedly connected to the left end of a rotating rod, and a plurality of stirring paddles are fixedly connected to the periphery of the outer side of the second connecting rod and the periphery of the outer side of the third connecting rod at equal intervals. According to the cell culture device, the motor can drive the second connecting rod to rotate clockwise, at the moment, the third connecting rod can rotate anticlockwise along with the second connecting rod, cells in the hollow barrel can make full contact with a nutrient solution, the situation that part of the cells make insufficient contact with the nutrient solution is not prone to occurring, and therefore the cell culture efficiency can be improved.
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Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a micro bioreactor for cell culture. Background Art

[0002] Cell culture plays a vital role in biomedical research, drug development, and cell therapy. With the rapid development of these fields, higher requirements are placed on the efficiency, reproducibility, and controllability of cell culture, as well as the ability to simulate the in vivo environment.

[0003] Micro bioreactors are needed for cell culture. They are widely used in the biopharmaceutical field because they can culture various types of cells and simulate the growth environment of cells in organisms.

[0004] When using the existing micro bioreactors on the market, the cells to be cultured are first placed in a culture vessel, and then a nutrient solution is poured into the culture vessel. Finally, a stirring rod is driven by a motor to rotate so that the cells come into contact with the culture solution. This can improve the quality of cell culture. However, this method only uses the motor to drive the stirring rod to rotate during stirring, which may easily lead to insufficient contact between some cells and the culture solution, thereby reducing the cell culture efficiency and failing to meet the needs of users. Utility Model Content

[0005] In order to make up for the above deficiencies, the present invention provides a micro bioreactor for cell culture, aiming to improve the problem of insufficient contact between cells and culture fluid in the micro bioreactor in the prior art.

[0006] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a micro-bioreactor for cell culture, comprising a hollow barrel, a barrel cover is provided on the top of the hollow barrel, the top of the barrel cover is fixedly connected to a motor, the output end of the motor passes through the barrel cover and is fixedly connected to a second connecting rod, the middle and lower part of the outer side of the second connecting rod is rotatably connected to a hollow column, the inner bottom of the hollow column is rotatably connected to a third connecting rod, the adjacent ends of the third connecting rod and the second connecting rod are fixedly connected to a first bevel gear, the right side of the inside of the hollow column is rotatably connected to a rotating rod, the left end of the rotating rod is fixedly connected to a second bevel gear, the two first bevel gears are meshed with the second bevel gear, a plurality of stirring paddles are fixedly connected at equal distances on all sides of the outer sides of the second connecting rod and the third connecting rod, and a cleaning mechanism is provided inside the hollow barrel, and the cleaning mechanism is used to facilitate cleaning of impurities remaining on the inner wall of the hollow barrel.

[0007] As a further description of the above technical solution:

[0008] The cleaning mechanism includes a first gear, which is fixedly connected to the upper middle part of the outer side of the second connecting rod. The left end of the inner top of the barrel cover is rotatably connected to the first connecting rod, and the bottom end of the first connecting rod is fixedly connected to the second gear. An annular hollow groove is provided in the upper middle part of the inner side of the hollow barrel, and a gear ring is rotatably connected to the inside of the annular hollow groove. A plurality of scrapers are fixedly connected to the bottom of the gear ring at equal intervals.

[0009] As a further description of the above technical solution:

[0010] The top front side of the barrel cover is connected with a feed port, the lower middle part of the right side of the hollow barrel is connected with a discharge port, and one end of the discharge port and the feed port is threadedly connected with a sealing cover.

[0011] As a further description of the above technical solution:

[0012] The left and right sides of the top of the barrel cover are fixedly connected with handles, and the outer sides of the two handles are fixedly connected with rubber sleeves.

[0013] As a further description of the above technical solution:

[0014] The left and right sides of the barrel cover are both threadedly connected with bolts, and one end of the bolt passes through the barrel cover and the hollow barrel in sequence.

[0015] As a further description of the above technical solution:

[0016] An observation window is provided in the middle of the front side of the hollow barrel, and a scale line is provided on the right side of the front end of the hollow barrel.

[0017] As a further description of the above technical solution:

[0018] A control panel is fixedly connected to the middle portion of the right side of the hollow barrel, and the control panel is electrically connected to the motor.

[0019] As a further description of the above technical solution:

[0020] The left and right sides of the middle portion of the top end of the barrel cover are both connected with air intake pipes, and the bottom end of the air intake pipes passes through the barrel cover.

[0021] The utility model has the following beneficial effects:

[0022] In the utility model, the motor can drive the second connecting rod to rotate clockwise, and the first bevel gear on the second connecting rod will rotate accordingly, and the second bevel gear meshed with it will also rotate, thereby driving the first bevel gear on the third connecting rod to rotate. At this time, the third connecting rod will rotate counterclockwise, so that the cells in the hollow barrel can be fully contacted with the nutrient solution, and it is less likely that some cells will not be in sufficient contact with the culture solution, thereby improving the cell culture efficiency and meeting the needs of users.

[0023] In the present invention, when the second connecting rod rotates, the first gear will rotate accordingly, and the second gear meshing with it will also rotate, thereby driving the gear ring to rotate. At the same time, the scraper at the bottom of the gear ring will rotate to clean the inner wall of the hollow barrel, which can prevent some cells from remaining on the inner wall of the hollow barrel and affecting the normal progress of the next culture work, thereby improving the practicality of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a three-dimensional diagram of the micro bioreactor for cell culture proposed in the present invention;

[0025] Figure 2 This is a cross-sectional view of the structure of the micro bioreactor for cell culture proposed in the present invention;

[0026] Figure 3 This is a partial structural cross-sectional view of the micro bioreactor for cell culture proposed in the present utility model;

[0027] Figure 4 This is a schematic diagram of the partial structure of the micro bioreactor for cell culture proposed in the present invention;

[0028] Figure 5 This is a cross-sectional view of the hollow barrel structure of the micro bioreactor for cell culture proposed in the present utility model.

[0029] Legend:

[0030] 1. Hollow barrel; 2. Cleaning mechanism; 201. First gear; 202. First connecting rod; 203. Second gear; 204. Annular hollow groove; 205. Gear ring; 206. Scraper; 3. Barrel cover; 4. Motor; 5. Second connecting rod; 6. Hollow column; 7. Third connecting rod; 8. First bevel gear; 9. Rotating rod; 10. Second bevel gear; 11. Agitator paddle; 12. Inlet pipe; 13. Bolt; 14. Handle; 15. Rubber sleeve; 16. Feed port; 17. Discharge port; 18. Sealing cover; 19. Observation window; 20. Scale line; 21. Control panel. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0032] Reference Figure 1 、 Figure 2 and Figure 3 The utility model provides an embodiment of a micro bioreactor for cell culture, comprising a hollow barrel 1, a barrel cover 3 is provided on the top of the hollow barrel 1, a motor 4 is fixedly connected to the top of the barrel cover 3, the output end of the motor 4 passes through the barrel cover 3 and is fixedly connected to a second connecting rod 5, the middle and lower part of the outer side of the second connecting rod 5 is rotatably connected to a hollow column 6, the inner bottom of the hollow column 6 is rotatably connected to a third connecting rod 7, the adjacent ends of the third connecting rod 7 and the second connecting rod 5 are fixedly connected to a first bevel gear 8, the hollow column 6 is fixedly connected to the first bevel gear 8, and the hollow column 6 is fixedly connected to the first bevel gear 8. The right side of the interior is rotatably connected to a rotating rod 9, the left end of the rotating rod 9 is fixedly connected to a second bevel gear 10, the two first bevel gears 8 are meshed with the second bevel gear 10, and the outer sides of the second connecting rod 5 and the third connecting rod 7 are equidistantly fixedly connected with a plurality of stirring paddles 11. A cleaning mechanism 2 is provided inside the hollow barrel 1. The cleaning mechanism 2 is used to facilitate cleaning of impurities remaining on the inner wall of the hollow barrel 1. Handles 14 are fixedly connected to the left and right sides of the top of the barrel cover 3, and rubber sleeves 15 are fixedly connected to the outer sides of the two handles 14.

[0033] Specifically, the second connecting rod 5 can be driven by the motor 4 to rotate. As the second connecting rod 5 rotates, the first bevel gear 8 will also rotate accordingly. At this time, the second bevel gear 10 engaged with the first bevel gear 8 will also rotate accordingly. At the same time, the first bevel gear 8 on the third connecting rod 7 can also rotate accordingly, and the third connecting rod 7 will rotate counterclockwise. Since the rotation directions of the second connecting rod 5 and the third connecting rod 7 are opposite, the stirring paddle 11 will also rotate in the opposite direction with the rotation of the rod. At this time, the various substances in the container can be more fully contacted and mixed during the stirring process, thereby achieving a more ideal stirring effect.

[0034] Reference Figure 2 、 Figure 4 and Figure 5 , the cleaning mechanism 2 includes a first gear 201, which is fixedly connected to the middle and upper part of the outer side of the second connecting rod 5, and the left end of the inner top of the barrel cover 3 is rotatably connected to the first connecting rod 202, and the bottom end of the first connecting rod 202 is fixedly connected to the second gear 203. An annular hollow groove 204 is provided in the middle and upper part of the inner side of the hollow barrel 1, and a gear ring 205 is rotatably connected inside the annular hollow groove 204. A plurality of scrapers 206 are fixedly connected at equal intervals around the bottom of the gear ring 205. The top front side of the barrel cover 3 is connected to the feed port 16, and the middle and lower right side of the hollow barrel 1 is connected to the discharge port 17. One end of the discharge port 17 and the feed port 16 is threadedly connected to a sealing cover 18. The left and right sides of the middle part of the top of the barrel cover 3 are connected to the air inlet pipe 12, and the bottom end of the air inlet pipe 12 passes through the barrel cover 3;

[0035] Specifically, various gases required for the reaction can be transported through the air inlet pipe 12, thereby obtaining different experimental results. The feed port 16 and the discharge port 17 can be controlled by loosening the sealing cover 18, so that the staff can add or discharge materials in time to control the reaction. While the second connecting rod 5 is driven to rotate by the motor 4, the first gear 201 on its outer side will rotate accordingly, and the second gear 203 engaged with it will also rotate, thereby driving the gear ring 205 to rotate. At the same time, the scraper 206 at the bottom of the gear ring 205 will rotate accordingly to clean the inner wall of the hollow barrel 1. The staff can perform the cleaning work more easily to ensure that there will be no residual impurities on the inner wall of the container, thereby affecting the next experiment.

[0036] Reference Figure 1 , an observation window 19 is provided in the middle of the front side of the hollow barrel 1, and a scale line 20 is provided on the right side of the front end of the hollow barrel 1;

[0037] Specifically, through the cooperation between the observation window 19 and the scale line 20, it is convenient for the staff to check the amount of nutrient solution inside the hollow barrel 1 at any time.

[0038] Reference Figure 1 and Figure 3 , the left and right sides of the barrel cover 3 are threadedly connected with bolts 13, one end of the bolt 13 passes through the barrel cover 3 and the hollow barrel 1 in sequence, and a control panel 21 is fixedly connected to the middle of the right side of the hollow barrel 1, and the control panel 21 is electrically connected to the motor 4;

[0039] Specifically, the barrel cover 3 can be removed by loosening the bolts 13, so that the staff can conveniently inspect and repair the structure at the bottom of the barrel cover 3. The operation of the motor 4 can be controlled through the control panel 21. The model of the motor 4 is Nanotec of Germany.

[0040] Working principle: When using the device, first put the cells to be cultured into the hollow barrel 1 through the feed port 16, then pour the nutrient solution into the hollow barrel 1 through the feed port 16, and then screw on the sealing cover 18 on the feed port 16. At this time, the motor 4 can drive the second connecting rod 5 to rotate clockwise, and the first bevel gear 8 on the second connecting rod 5 will rotate accordingly, and the second bevel gear 10 meshing with it will also rotate, thereby driving the first bevel gear 8 on the third connecting rod 7 to rotate. At this time, the third connecting rod 7 will rotate counterclockwise. Since the second connecting rod 5 and the third connecting rod 7 rotate in opposite directions, the stirring paddles 11 on the second connecting rod 5 and the third connecting rod 7 will rotate in opposite directions. Therefore, the cells in the hollow barrel 1 can be fully contacted with the nutrient solution, and it is not easy for some cells to have insufficient contact with the culture solution.

[0041] When the motor 4 drives the second connecting rod 5 to rotate, the first gear 201 on its outer side will rotate accordingly, and the second gear 203 engaged with it will also rotate, thereby driving the gear ring 205 to rotate. At the same time, the scraper 206 at the bottom of the gear ring 205 will rotate accordingly to clean the inner wall of the hollow barrel 1, thereby preventing some cells from remaining on the inner wall of the hollow barrel 1 and affecting the normal progress of the next culture work.

[0042] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent replacements for some of the technical features therein. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A micro bioreactor for cell culture, comprising a hollow barrel (1), characterized in that: The top of the hollow barrel (1) is provided with a barrel cover (3), the top of the barrel cover (3) is fixedly connected to a motor (4), the output end of the motor (4) passes through the barrel cover (3) and is fixedly connected to a second connecting rod (5), the middle and lower outer portion of the second connecting rod (5) is rotatably connected to a hollow column (6), the inner bottom of the hollow column (6) is rotatably connected to a third connecting rod (7), the adjacent ends of the third connecting rod (7) and the second connecting rod (5) are both fixedly connected to a first bevel gear (8), the The right side of the interior of the hollow column (6) is rotatably connected to a rotating rod (9), the left end of the rotating rod (9) is fixedly connected to a second bevel gear (10), the two first bevel gears (8) are meshed and connected to the second bevel gear (10), and a plurality of stirring paddles (11) are fixedly connected at equal intervals on the outer sides of the second connecting rod (5) and the third connecting rod (7). A cleaning mechanism (2) is provided inside the hollow barrel (1), and the cleaning mechanism (2) is used to facilitate cleaning of impurities remaining on the inner wall of the hollow barrel (1).

2. The micro-bioreactor for cell culture according to claim 1, characterized in that: The cleaning mechanism (2) comprises a first gear (201), the first gear (201) being fixedly connected to the middle and upper portion of the outer side of the second connecting rod (5), the first connecting rod (202) being rotatably connected to the left end of the inner top of the barrel cover (3), the second gear (203) being fixedly connected to the bottom end of the first connecting rod (202), an annular hollow groove (204) being provided at the middle and upper portion of the inner side of the hollow barrel (1), a gear ring (205) being rotatably connected inside the annular hollow groove (204), and a plurality of scrapers (206) being fixedly connected to the bottom of the gear ring (205) at equal intervals.

3. The micro-bioreactor for cell culture according to claim 1, characterized in that: The top front side of the barrel cover (3) is connected to a feed port (16), and the lower middle portion of the right side of the hollow barrel (1) is connected to a discharge port (17). One end of the discharge port (17) and the feed port (16) are threadedly connected to a sealing cover (18).

4. The micro-bioreactor for cell culture according to claim 1, characterized in that: The left and right sides of the top of the barrel cover (3) are both fixedly connected with handles (14), and the outer sides of the two handles (14) are both fixedly connected with rubber sleeves (15).

5. The micro-bioreactor for cell culture according to claim 1, characterized in that: Bolts (13) are threadedly connected to the left and right sides of the barrel cover (3), and one end of the bolt (13) passes through the barrel cover (3) and the hollow barrel (1) in sequence.

6. The micro-bioreactor for cell culture according to claim 1, characterized in that: An observation window (19) is provided in the middle of the front side of the hollow barrel (1), and a scale line (20) is provided on the right side of the front end of the hollow barrel (1).

7. The micro-bioreactor for cell culture according to claim 1, characterized in that: A control panel (21) is fixedly connected to the middle portion of the right side of the hollow barrel (1), and the control panel (21) is electrically connected to the motor (4).

8. The micro-bioreactor for cell culture according to claim 1, characterized in that: The left and right sides of the middle portion of the top of the barrel cover (3) are both connected to an air intake pipe (12), and the bottom end of the air intake pipe (12) passes through the barrel cover (3).