In-vitro detection device for skin external medicine

Abstract

The utility model discloses an in-vitro detection device for skin external medicines, relates to the technical field of medical instruments, and provides the following scheme that the in-vitro detection device comprises a detection box, and a lower connecting chamber and an upper connecting chamber are arranged, so that when the detection device is used for detecting the skin external medicines, the lower connecting chamber is clamped with the detection box; under the connection of a second inserting block and a second inserting groove, the connected upper chamber and the polystyrene thin plate are clamped, meanwhile, the taken-down skin tissue is inserted into a circular cavity of the upper chamber, the epidermis side faces upwards, the dermis side faces downwards, the upper chamber is fixed to the polystyrene thin plate, and the lower chamber is filled with a culture medium of a proper volume and covers dermis; a proper amount of to-be-detected external medicine is dropwise added on the epidermis, the sealing cover is closed, the detection box is kept in a sterile state, the medicine cannot leak to the lower chamber to affect the result due to blocking of the sealing film connected with the bottom of the upper chamber, tissue is taken for next detection after a proper time, and the effect of effectively detecting the skin external medicine is achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, in particular to an in vitro detection device for external skin medications. Background Art

[0002] Topical medications are the cornerstone and foundation of treatment options for many skin diseases. Currently, the development of new topical medications is booming in clinical practice, making it a hot topic in the field of skin research. Cellular and animal experiments are essential preclinical validation processes for new drug development and are essential for advancing the development of human medicine and life sciences. However, skin tissue has a unique anatomical structure, with the stratum corneum as the outermost layer of the epidermis, which affects the transdermal penetration of most drugs. Therefore, the effectiveness of cell experiments is not directly equivalent to in vivo effectiveness. Although animal models can further confirm the efficacy and safety of new topical medications, human and animal tissues have significant differences in physiological functions and anatomical structures. During the development of new drugs, it is not uncommon for animal experiments to be effective while human trials are ineffective. Therefore, a convenient and effective in vitro model based on human skin lesions to evaluate the efficacy of topical medications is of great significance for the development of skin topical medications, especially for inflammatory skin diseases such as psoriasis and atopic dermatitis.

[0003] When using the in vitro drug detection device in the prior art, the drug detection device is not very convenient for directly detecting human skin tissue, which causes deviations in the drug use effect when performing daily in vitro drug detection and does not meet people's usage needs. Therefore, an in vitro detection device for external skin drugs is needed. Utility Model Content

[0004] The purpose of the utility model is to provide an in vitro detection device for externally applied skin drugs, which solves the problem in the prior art that drug detection devices are not convenient for directly detecting human skin tissue.

[0005] To achieve the above-mentioned objectives, the utility model provides the following technical solutions: an in vitro detection device for external skin drugs, comprising a detection box, the outer wall of the detection box being detachably connected to a connecting lower chamber, the outer wall of the connecting lower chamber being fixedly connected to a first plug-in block slidably connected to the outer wall of the detection box, a first slot being provided at the connection portion between the outer wall of the detection box and the first plug-in block, the outer wall of the connecting lower chamber being detachably connected to an upper chamber, the outer wall of the connecting upper chamber being fixedly connected to a second plug-in block slidably connected to the outer wall of the connecting lower chamber, a second slot being provided at the connection portion between the outer wall of the connecting lower chamber and the second plug-in block, a sealing film being provided at the bottom of the connecting upper chamber, the outer wall of the detection box being rotatably connected to a sealing cover by a hinge, a placement hole being provided on the inner side wall of the connecting upper chamber, the skin epidermis being plugged into the inner wall of the placement hole, a polystyrene sheet being provided inside the connecting lower chamber, one end of the skin epidermis extending to the skin dermis below the polystyrene sheet.

[0006] Preferably, the inner wall of the lower chamber connected to the upper chamber is provided with culture medium.

[0007] Preferably, the sealing film is composed of a mixture of wax and polyolefin.

[0008] Preferably, the inner wall size of the placement hole is 4 mm, and the inner wall contour of the placement hole and the skin punch are matched with each other.

[0009] Preferably, the culture medium between the connecting lower chamber and the connecting upper chamber covers the surface of the skin dermis, and the closed state of the sealing cover can make the detection state of the detection box sterile.

[0010] Preferably, the first plug blocks and first slots are provided in four groups, and the four groups of the first plug blocks and first slots are distributed around the detection box, and the second plug blocks and second slots are symmetrically arranged on both sides of the upper chamber.

[0011] Compared with the prior art, the beneficial effects of the present invention are:

[0012] By setting up a connecting lower chamber and a connecting upper chamber, when the skin topical medicine is tested and used through the detection device, the lower chamber is connected to the detection box through the connection of the first plug block and the first slot, and the upper chamber is connected to the polystyrene sheet through the connection of the second plug block and the second slot. At the same time, the removed skin tissue is inserted into the circular hole of the upper chamber with the epidermis side facing up and the dermis side facing down. The upper chamber is fixed to the polystyrene sheet, and the lower chamber is filled with an appropriate volume of culture medium to cover the dermis. An appropriate amount of topical medicine to be tested is added to the epidermis, and the sealing cover is closed to keep the detection box sterile. The medicine will not leak into the lower chamber and affect the result due to the membrane barrier. After an appropriate time, the tissue is taken for the next step of testing to achieve the effect of effective detection of skin topical medicine. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a schematic diagram of the overall structure of an in vitro detection device for topical skin medications proposed by the present invention;

[0014] Figure 2 This is a schematic diagram of the overall internal cross-sectional structure of an in vitro detection device for external skin medications proposed by the present invention;

[0015] Figure 3 This is a schematic diagram of the distribution structure of the placement holes of an in vitro detection device for external skin drugs proposed by the present invention;

[0016] Figure 4 This is a schematic diagram of the position distribution structure of the first plug-in block and the second plug-in block of an in vitro detection device for external skin drugs proposed by the present invention.

[0017] In the figure: 1, detection box; 2, connection to the lower chamber; 3, first plug; 4, first slot; 5, connection to the upper chamber; 6, second plug; 7, second slot; 8, sealing film; 9, placement hole; 10, skin epidermis; 11, skin dermis; 12, sealing cover; 13, polystyrene sheet. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0019] Examples, such as Figure 1-4 As shown, an in vitro detection device for topical skin drugs in the figure includes a detection box 1, the outer wall of the detection box 1 is detachably connected to a connection lower chamber 2, the outer wall of the connection lower chamber 2 is fixedly connected to a first plug block 3 that is slidably connected to the outer wall of the detection box 1, a first slot 4 is provided at the connection portion between the outer wall of the detection box 1 and the first plug block 3, the outer wall of the connection lower chamber 2 is detachably connected to a connection upper chamber 5, the outer wall of the connection upper chamber 5 is fixedly connected to a second plug block 6 that is slidably connected to the outer wall of the connection lower chamber 2, a second slot 7 is provided at the connection portion between the outer wall of the connection lower chamber 2 and the second plug block 6, a sealing film 8 is provided at the bottom of the connection upper chamber 5, the outer wall of the detection box 1 is rotatably connected to a sealing cover 12 by a hinge, a placement hole 9 is provided on the inner side wall of the connection upper chamber 5, a skin epidermis 10 is inserted into the inner wall of the placement hole 9, a polystyrene sheet 13 is provided inside the connection lower chamber (2), and one end of the skin epidermis 10 extends to the skin dermis 11 below the polystyrene sheet 13.

[0020] Among them, such as Figure 3 As shown, the inner wall of the lower chamber 2 is located below the upper chamber 5 and is provided with a culture medium, which is conducive to convenient detection of in vitro drugs.

[0021] Among them, such as Figure 2 As shown, the sealing film 8 is composed of a mixture of wax and polyolefin, which is beneficial to the setting that the sealing film 8 is composed of a mixture of wax and polyolefin. The sealing film has a certain elasticity and can better adhere to the skin tissue, so that the drug will not leak into the lower chamber and affect the results because of the membrane barrier.

[0022] Among them, such as Figure 3 As shown, the inner wall size of the placement hole 9 is 4 mm, and the inner wall contour of the placement hole 9 and the skin puncher are matched with each other, which is beneficial to facilitate the placement of the removed skin by the skin puncher through the setting of the inner wall size of the placement hole 9 being 4 mm.

[0023] Among them, such as Figure 2 As shown, the culture medium between the connecting lower chamber 2 and the connecting upper chamber 5 covers the surface of the skin dermis 11, and the closed state of the sealing cover 12 can make the detection state of the detection box 1 sterile, which is beneficial for covering the surface of the skin dermis 11 through the culture medium between the connecting lower chamber 2 and the connecting upper chamber 5, thereby facilitating contact between the skin and the drug.

[0024] Among them, such as Figure 4 As shown, four groups of first plug-in blocks 3 and first slots 4 are provided, and the positions of the four groups of first plug-in blocks 3 and first slots 4 are distributed around the detection box 1. The second plug-in blocks 6 and the second slots 7 are symmetrically arranged on both sides of the upper chamber 5. It is beneficial to set four groups of first plug-in blocks 3 and first slots 4 distributed around the detection box 1, so as to facilitate the connection between the upper chamber and the lower chamber and the detection box 1.

[0025] During use: When the detection device is used to detect external drugs for skin use, the lower chamber 2 and the detection box 1 are connected by connecting the first plug block 3 and the first slot 4, and the upper chamber 5 and the polystyrene sheet 13 are connected by connecting the second plug block 6 and the second slot 7. At the same time, the removed skin tissue is inserted into the circular hole of the upper chamber with the epidermis side facing up and the dermis side facing down. The upper chamber is fixed to the polystyrene sheet, and the lower chamber is filled with an appropriate volume of culture medium to cover the dermis. An appropriate amount of external drugs to be tested is added to the epidermis, and the sealing cover 12 is closed to keep the detection box 1 sterile. The drugs will not leak into the lower chamber and affect the results due to the membrane barrier. After an appropriate time, the tissue is taken for the next step of testing to achieve the effect of effective detection of external drugs for skin use.

[0026] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0027] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An in vitro detection device for topical skin medication, comprising a detection box (1), characterized in that: The outer wall of the detection box (1) is detachably connected to the connecting lower chamber (2), the outer wall of the connecting lower chamber (2) is fixedly connected to a first plug-in block (3) that is slidably connected to the outer wall of the detection box (1), a first slot (4) is provided at the connection portion between the outer wall of the detection box (1) and the first plug-in block (3), the outer wall of the connecting lower chamber (2) is detachably connected to the connecting upper chamber (5), the outer wall of the connecting upper chamber (5) is fixedly connected to a second plug-in block (6) that is slidably connected to the outer wall of the connecting lower chamber (2), the outer wall of the connecting lower chamber (2) and the second plug-in block A second slot (7) is provided at the connection portion of (6), a sealing film (8) is provided at the bottom of the upper connection chamber (5), the outer wall of the detection box (1) is connected to a sealing cover (12) by a hinge, a placement hole (9) is provided on the inner side wall of the upper connection chamber (5), the skin epidermis (10) is inserted into the inner wall of the placement hole (9), a polystyrene sheet (13) is provided inside the lower connection chamber (2), and one end of the skin epidermis (10) extends to the skin dermis (11) below the polystyrene sheet (13).

2. The in vitro detection device for externally applied skin drugs according to claim 1, characterized in that: The inner wall of the connection lower chamber (2) is located below the connection upper chamber (5) and is provided with a culture medium.

3. The in vitro detection device for topical skin medication according to claim 1, characterized in that: The sealing film (8) is composed of a mixture of wax and polyolefin.

4. The in vitro detection device for externally applied skin drugs according to claim 1, characterized in that: The inner wall size of the placement hole (9) is 4 mm, and the inner wall contour of the placement hole (9) and the skin punch are matched with each other.

5. The in vitro detection device for externally applied skin drugs according to claim 1, characterized in that: The culture medium between the connecting lower chamber (2) and the connecting upper chamber (5) covers the surface of the skin dermis (11), and the closed state of the sealing cover (12) can make the detection state of the detection box (1) sterile.

6. The in vitro detection device for externally applied skin drugs according to claim 1, characterized in that: The first plug-in block (3) and the first slot (4) are each provided in four groups, and the positions of the four groups of the first plug-in block (3) and the first slot (4) are distributed around the detection box (1), and the second plug-in block (6) and the second slot (7) are both symmetrically arranged on both sides of the connection chamber (5).

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