High-density suspension cell culture oxygenation shake flask

Abstract

The utility model discloses a high-density suspension cell culture oxygenation shake flask which comprises a culture shake flask, an oxygenation structure is in butt joint with the culture shake flask, an installation structure is in butt joint with the oxygenation structure, one end of the oxygenation structure is connected with a pressurization structure, and the installation structure comprises a sealing cover, a movable plate, an LED lamp tube and a vent hole. The oxygenation structure comprises a mounting pipe, a first culture tank, a second culture tank, a partition plate, a connecting sleeve, a first filter membrane, a second filter membrane and a partition ring, and the pressurization structure comprises a lead-in valve, a lead-out valve, a transition pipe, a ventilation pipe, a partition membrane, a sleeve rod, a transmission rod, a sleeve ring and a butt joint port. The culture shake flask disclosed by the utility model has the beneficial effects that biological oxygen production is realized through the arranged oxygen increasing structure, the frequency of artificial oxygen increasing is reduced, meanwhile, the pressurizing effect is improved through the arranged pressurizing structure, and generated oxygen can be more quickly guided into the culture shake flask.

Description

Technical Field

[0001] The utility model relates to the technical field of suspended cell culture, in particular to a high-density suspended cell culture oxygenation shake flask. Background Art

[0002] Unlike plant and microbial cells, animal cells lack cell walls and are therefore very sensitive to shear forces during culture. Cellular growth, development, division, reproduction, and other vital activities require the consumption of large quantities of oxygen, participating in the tricarboxylic acid cycle. However, traditional culture shake flasks have narrow necks and deep, large bottoms, severely hindering gas flow. The flasks rotate along their central axis on a shaker, resulting in laminar flow, which is not conducive to mixing and oxygen transfer.

[0003] The existing shake flask generally provides a total amount of oxygen to the culture of high-density suspended cells by artificial oxygenation. This oxygenation method requires strict manual monitoring to prevent the problem of hypoxia caused by insufficient oxygen, which is labor-intensive. Utility Model Content

[0004] In view of the problems in the related art, the present invention proposes a high-density suspension cell culture oxygenation shake flask to overcome the above technical problems existing in the existing related art.

[0005] To this end, the specific technical solutions adopted in this utility model are as follows:

[0006] A high-density suspended cell culture oxygenation shake flask comprises a culture shake flask, an oxygenation structure is docked on the culture shake flask, a mounting structure is docked on the oxygenation structure, and one end of the oxygenation structure is connected to a pressurization structure.

[0007] Furthermore, the installation structure includes a cover, a movable plate, an LED light tube, and a vent hole. The cover is movably connected to the movable plate, the bottom end of the movable plate is connected to the LED light tube, and a vent hole is opened in the middle of the movable plate.

[0008] Furthermore, the oxygen enrichment structure includes a mounting tube, a first culture tank, a second culture tank, a partition plate, a connecting sleeve, a first filter membrane, a second filter membrane, and a partition ring. The first culture tank is opened in the mounting tube, and the second culture tank is provided on one side of the first culture tank.

[0009] Furthermore, a partition plate is provided between the second culture tank and the first culture tank, a connecting sleeve is fixedly installed on the partition plate, a first slot hole is opened in the first culture tank, a second slot hole is opened in the second culture tank, a first filter membrane is installed in the first slot hole, a second filter membrane is installed in the second slot hole, and a partition ring is flatly embedded on the inner wall of the mounting tube.

[0010] Furthermore, the boosting structure includes an inlet valve, an outlet valve, a transition pipe, a vent pipe, a partition membrane, a sleeve rod, a transmission rod, a collar, and a docking port. An outlet valve is provided on one side of the inlet valve. Both the inlet valve and the outlet valve are connected to a transition pipe, and a partition membrane is provided in the transition pipe.

[0011] Furthermore, a docking port is provided on the transition pipe, the docking port is connected to the vent pipe, the vent pipe is installed in the connecting sleeve, the vent pipe is connected to the vent hole, and one side of the partition membrane is rotatably connected to the transmission rod.

[0012] Furthermore, the peripheral side of the partition membrane is fixedly connected to the transition pipe, and a fixing plate is fixed between the two ends of the partition membrane and the transition pipe. A sleeve rod is fixedly installed on the fixing plate, and a sleeve ring is slidably sleeved on the sleeve rod, and the sleeve ring is rotatably connected to one end of the transmission rod.

[0013] The beneficial effects of the present invention are as follows: the culture shake flask of the present invention realizes biological oxygen production through the oxygenation structure, reduces the frequency of artificial oxygenation, and at the same time, the pressurization structure improves the pressurization effect, and the generated oxygen can be introduced into the culture shake flask more quickly. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0015] Figure 1 This is a schematic diagram of the main structure of a high-density suspension cell culture oxygenation shake flask according to an embodiment of the utility model;

[0016] Figure 2 This is a schematic diagram of the installation structure of a high-density suspension cell culture oxygenation shake flask according to an embodiment of the utility model;

[0017] Figure 3 This is a schematic diagram of the oxygenation structure of a high-density suspension cell culture oxygenation shake flask according to an embodiment of the utility model;

[0018] Figure 4 This is a schematic diagram of a pressurization structure of a high-density suspension cell culture oxygenation shake flask according to an embodiment of the utility model;

[0019] Figure 5 This is a schematic diagram of a transition tube for a high-density suspension cell culture oxygenated shake flask according to an embodiment of the present invention;

[0020] Figure 6 This is a schematic diagram of a partition membrane for a high-density suspension cell culture oxygenation shake flask according to an embodiment of the present utility model;

[0021] Figure 7 The present invention is a schematic diagram of a sleeve rod of a high-density suspension cell culture oxygenation shake flask according to an embodiment of the present invention.

[0022] In the picture:

[0023] 1. Culture shake flask; 2. Mounting structure; 201. Sealing cover; 202. Movable plate; 203. LED lamp; 204. Vent; 3. Oxygenation structure; 301. Mounting tube; 302. First culture tank; 303. Second culture tank; 304. Partition plate; 305. Connecting sleeve; 306. First filter membrane; 307. Second filter membrane; 308. Partitioning ring; 4. Pressurization structure; 401. Inlet valve; 402. Outlet valve; 403. Transition pipe; 404. Vent; 405. Partitioning membrane; 406. Sleeve rod; 407. Transmission rod; 408. Sleeve ring; 409. Docking port. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0025] According to an embodiment of the present utility model, a high-density suspension cell culture oxygenation shake flask is provided.

[0026] Embodiment 1;

[0027] like Figure 1-7 As shown, the high-density suspension cell culture oxygenation shake flask according to the embodiment of the present invention includes a culture shake flask 1, an oxygenation structure 3 is docked on the culture shake flask 1, an installation structure 2 is docked on the oxygenation structure 3, and one end of the oxygenation structure 3 is connected to a pressurizing structure 4. The oxygenation structure 3 on the culture shake flask 1 can provide stable oxygen through biological oxygen production and can effectively treat carbon dioxide. The pressurizing structure 4 uses the pressure difference and the centrifugal force of the rotation of the shake flask to increase the pressure, so that the oxygen can be extracted faster.

[0028] The mounting structure 2 includes a cover 201, a movable plate 202, an LED lamp tube 203, and a vent 204. The cover 201 is movably connected with the movable plate 202, the bottom end of the movable plate 202 is connected to the LED lamp tube 203, and the middle part of the movable plate 202 is provided with a vent 204. The oxygenation structure 3 includes a mounting tube 301, a first culture tank 302, a second culture tank 303, a partition plate 304, a connecting sleeve 305, a first filter membrane 306, a second filter membrane 307, and a partition ring 308. The first culture tank 302 is provided in the mounting tube 301, the second culture tank 303 is provided on one side of the first culture tank 302, a partition plate 304 is provided between the second culture tank 303 and the first culture tank 302, a connecting sleeve 305 is fixedly installed on the partition plate 304, and the first culture tank 302 is provided with a The first slot hole and the second culture tank 303 are provided with a second slot hole. A first filter membrane 306 is installed in the first slot hole, and a second filter membrane 307 is installed in the second slot hole. A partition ring 308 is flatly embedded on the inner wall of the mounting tube 301. The two culture tanks separated by the mounting tube 301 of the oxygenation structure 3 are respectively used to place Chlorella. Chlorella grows rapidly and has a relatively high photosynthetic efficiency. It can produce stable oxygen by absorbing carbon dioxide. The first filter membrane 306 is a silicone rubber membrane that can effectively and selectively transmit oxygen, and the second filter membrane 307 is a polyamide membrane that can effectively and selectively transmit carbon dioxide. The first filter membrane 306 is correspondingly connected to the inlet valve 401, and the second filter membrane 307 is correspondingly connected to the outlet valve 402. The Chlorella performs photosynthesis under the illumination of the LED lamp tube 203 of the mounting structure 2.

[0029] The boost structure 4 includes an inlet valve 401, an outlet valve 402, a transition pipe 403, a vent pipe 404, a partition membrane 405, a sleeve rod 406, a transmission rod 407, a collar 408, and a docking port 409. The outlet valve 402 is provided on one side of the inlet valve 401. The inlet valve 401 and the outlet valve 402 are both connected to a transition pipe 403. A partition membrane 405 is provided in the transition pipe 403. A docking port 409 is provided on the transition pipe 403. The port 409 is connected to the vent pipe 404, which is installed in the connecting sleeve 305. The vent pipe 404 is connected to the vent hole 204. One side of the partition membrane 405 is rotatably connected to the transmission rod 407. The peripheral side of the partition membrane 405 is fixedly connected to the transition pipe 403. A fixing plate is fixed between the two ends of the partition membrane 405 and the transition pipe 403. A sleeve rod 406 is fixedly installed on the fixing plate. A sleeve ring 408 is slidably sleeved on the sleeve rod 406. The ring 408 rotates to connect one end of the transmission rod 407. The transmission rod 407 of the boosting structure 4 is fixedly installed on the fixed plate and is located on one side of the partition membrane 405. The partition membrane 405 itself has a certain degree of contractility. When the shaking bottle rotates, since one end of the transmission rod 407 is connected to the middle part of one side of the partition membrane 405, and both ends of the transmission rod 407 are made of metal with higher density, one end of the transmission rod 407 will drive the partition membrane 405 to move circularly toward the shaking bottle, thereby reducing the effective gas guide space in the transition tube 403 and increasing its gas pressure, thereby introducing oxygen from the inlet valve 401 into the shaking bottle, and carbon dioxide is introduced into the mounting tube 301 through the outlet valve 402. When the shaking bottle stops rotating, the transmission rod 407 is reset under the action of the gravity of the collar 408 to complete the pressurization. The inlet valve 401 and the outlet valve 402 are both one-way valves, but their directions are opposite.

[0030] In order to facilitate understanding of the above technical solutions of the present invention, the working principle or operation method of the present invention in actual process is described in detail below.

[0031] The oxygenation structure 3 on the culture shake flask 1 can provide stable oxygen through biological oxygen production and can effectively treat carbon dioxide. The pressurization structure 4 uses the pressure difference and the centrifugal force of the shake flask to increase the pressure, so that the oxygen can be extracted faster. The two culture tanks separated by the mounting tube 301 of the oxygenation structure 3 are used to place Chlorella, which grows fast and has a fast photosynthetic efficiency. It can produce stable oxygen by absorbing carbon dioxide. The first filter membrane 306 is a silicone rubber membrane that can effectively and selectively pass oxygen, and the second filter membrane 307 is a polyamide membrane that can effectively and selectively pass carbon dioxide. The first filter membrane 306 is connected to the inlet valve 401, and the second filter membrane 307 is connected to the outlet valve 402. Chlorella passes through the LE of the mounting structure 2 The light from the D lamp 203 is used for photosynthesis. The transmission rod 407 of the pressurization structure 4 is fixedly mounted on the fixed plate and is located on one side of the partition membrane 405. The partition membrane 405 itself has a certain degree of shrinkage. When the shaking bottle rotates, one end of the transmission rod 407 is connected to the middle part of one side of the partition membrane 405, and both ends of the transmission rod 407 are made of metal with a relatively high density. Therefore, one end of the transmission rod 407 will drive the partition membrane 405 to move circularly toward the shaking bottle, thereby reducing the effective gas guide space in the transition tube 403 and increasing its gas pressure, thereby introducing oxygen from the inlet valve 401 into the shaking bottle, and introducing carbon dioxide into the mounting tube 301 through the outlet valve 402 to complete the pressurization. The inlet valve 401 and the outlet valve 402 are both one-way valves, but their directions are opposite.

[0032] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A high-density suspension cell culture oxygenated shake flask, characterized in that: The invention comprises a culture shake flask (1), wherein an oxygenation structure (3) is docked on the culture shake flask (1), a mounting structure (2) is docked on the oxygenation structure (3), and one end of the oxygenation structure (3) is connected to a pressurization structure (4).

2. A high-density suspension cell culture oxygenated shake flask according to claim 1, characterized in that: The mounting structure (2) comprises a cover (201), a movable plate (202), an LED light tube (203), and a vent (204); the cover (201) is movably connected to the movable plate (202); the bottom end of the movable plate (202) is connected to the LED light tube (203); and the middle of the movable plate (202) is provided with a vent (204).

3. A high-density suspension cell culture oxygenated shake flask according to claim 2, characterized in that: The oxygenation structure (3) includes a mounting tube (301), a first culture tank (302), a second culture tank (303), a partition plate (304), a connecting sleeve (305), a first filter membrane (306), a second filter membrane (307), and a partition ring (308). The first culture tank (302) is provided in the mounting tube (301), and a second culture tank (303) is provided on one side of the first culture tank (302).

4. A high-density suspension cell culture oxygenated shake flask according to claim 3, characterized in that: A partition plate (304) is provided between the second culture tank (303) and the first culture tank (302), a connecting sleeve (305) is fixedly installed on the partition plate (304), a first slot hole is provided in the first culture tank (302), a second slot hole is provided in the second culture tank (303), a first filter membrane (306) is installed in the first slot hole, a second filter membrane (307) is installed in the second slot hole, and a partition ring (308) is flatly embedded on the inner wall of the installation tube (301).

5. A high-density suspension cell culture oxygenated shake flask according to claim 4, characterized in that: The boosting structure (4) comprises an inlet valve (401), an outlet valve (402), a transition pipe (403), a vent pipe (404), a partition membrane (405), a sleeve rod (406), a transmission rod (407), a collar (408), and a docking port (409). The outlet valve (402) is provided on one side of the inlet valve (401). The inlet valve (401) and the outlet valve (402) are both connected to a transition pipe (403). The partition membrane (405) is provided in the transition pipe (403).

6. A high-density suspension cell culture oxygenated shake flask according to claim 5, characterized in that: The transition pipe (403) is provided with a docking port (409), the docking port (409) is connected to the vent pipe (404), the vent pipe (404) is installed in the connecting sleeve (305), the vent pipe (404) is connected to the vent hole (204), and one side of the partition membrane (405) is rotatably connected to the transmission rod (407).

7. A high-density suspension cell culture oxygenated shake flask according to claim 6, characterized in that: The peripheral side of the partition membrane (405) is fixedly connected to the transition tube (403), and a fixing plate is fixedly provided between the two ends of the partition membrane (405) and the transition tube (403). A sleeve rod (406) is fixedly installed on the fixing plate, and a sleeve ring (408) is slidably sleeved on the sleeve rod (406), and the sleeve ring (408) is rotatably connected to one end of the transmission rod (407).

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