NGS automatic pipetting analysis equipment

Abstract

The utility model relates to the technical field of biological pipetting equipment, in particular to NGS automatic pipetting analysis equipment which comprises a support, an X-axis linear module, a Z-axis linear module and a storage rack, the top of the support is fixedly connected with the X-axis linear module, the moving end of the X-axis linear module is fixedly connected with the Z-axis linear module, and the moving end of the Z-axis linear module is connected with a supporting plate; the supporting plate is fixedly connected with an electric cylinder, a pipettor and a separating part, an air cylinder connected with the electric cylinder is arranged in the pipettor, an air pipe of the air cylinder is communicated with a communicating box, the communicating box is connected with a plurality of air pipe connectors, rubber rings are arranged at the tail ends of all the air pipe connectors, and the outer walls of the rubber rings are in plug shapes matched with suction heads; the separating plate is provided with a through hole matched with the air pipe connector, the diameter of the through hole is smaller than that of the suction head, an electromagnet is arranged on the lower side of the separating plate and fixedly connected with the supporting plate, and the suction head and the pipettor are simple in connecting structure and convenient to control, and production cost is reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of biological liquid transfer equipment, and in particular relates to an NGS automated liquid transfer analysis device. Background Art

[0002] NGS, also known as high-throughput sequencing technology, relies on reading sequence information during PCR amplification of DNA using signals emitted by chemical markers when bases are inserted into the DNA chain. This signal can be either a light signal or an H+ flux signal. NGS offers the advantages of high throughput, high accuracy, and rich information. It can accurately locate genes in a short time and detect unknown sequences. NGS technology can sequence multiple samples simultaneously, significantly improving sequencing efficiency and reducing sequencing costs. Therefore, it is widely used in various research fields, including genomics, transcriptomics, and epigenetics.

[0003] NGS technology mainly includes four steps: library preparation, DNA cluster generation, sequencing, and data analysis. During the preparation process, pipetting equipment is required. Automated pipetting equipment includes a pipette, a placement platform, an X-axis linear module, and a Z-axis linear module. It can move along the X and Z axes to deliver the pipette to a designated location. It is an instrument that can automatically complete operations such as liquid aspiration, transfer, and discharge. It can automatically complete pipetting tasks according to preset programs without manual intervention, greatly improving experimental efficiency.

[0004] In order to automatically complete operations such as liquid absorption, transfer and discharge, existing automated pipetting equipment designs the tips and pipettes into a sealed snap-fit structure. Although this connection is stable, the structure is complex, which not only affects the structural stability but also makes replacement inconvenient. Utility Model Content

[0005] The purpose of the utility model is to provide an NGS automated pipetting analysis device with a simple connection structure between the tip and the pipette, and convenient control to reduce production costs.

[0006] In order to achieve the above technical objectives, the technical solutions adopted by this utility model are as follows:

[0007] An NGS automated pipetting analysis device comprises a bracket, an X-axis linear module, a Z-axis linear module and a storage rack, wherein the top of the bracket is fixedly connected to the X-axis linear module, the movable end of the X-axis linear module is fixedly connected to the Z-axis linear module, and the movable end of the Z-axis linear module is connected to a support plate;

[0008] The storage rack is provided with a trash box, a suction tip box, a sampling box and a sample placement box, and the suction tip box is provided with suction tips;

[0009] The support plate is fixedly connected to an electric cylinder, a pipette and a separator. The pipette is provided with an air cylinder connected to the electric cylinder. The air pipe of the cylinder is connected to a connecting box. The connecting box is connected to multiple air pipe interfaces. All the air pipe interfaces are provided with rubber rings at the ends. The outer wall of the rubber ring is in the shape of a plug that matches the suction head. The separator includes a separation plate. The separation plate is provided with a through hole that matches the air pipe interface. The diameter of the through hole is smaller than the diameter of the suction head. An electromagnet is provided on the lower side of the separation plate, and the electromagnet is fixedly connected to the support plate.

[0010] Wherein, a solenoid valve is provided between the connecting box and each air pipe interface.

[0011] Wherein, the storage rack is provided with a positioning seat, and the suction head box, sampling box and placement box are detachably fixedly connected above the positioning seat.

[0012] There are two electromagnets, and the two electromagnets are respectively arranged on both sides of the separation plate.

[0013] Wherein, a limit block is respectively provided on both sides above the separation plate, and the limit block is fixedly connected to the support plate.

[0014] Wherein, the trachea interface is detachably connected to the outer wall of the pipette.

[0015] The X-axis linear module is fixed on the top of the bracket. The X-axis linear module can control the horizontal movement of the pipette, and the Z-axis linear module controls the longitudinal movement of the pipette. The Z-axis linear module can be used to insert the tracheal interface into the suction head fixed in the suction head box, so that it is tightly connected through the rubber ring. The electric cylinder is energized to control the extension and contraction, thereby controlling the suction and discharge amount of the suction head. The cylinder is affected by the action of the electric cylinder to inhale or discharge gas, thereby controlling the suction and discharge of the suction head. The connecting box connects the tracheal interface to the cylinder, and all installed suction heads are uniformly controlled by the cylinder. The outer wall of the rubber ring The plug shape matches the suction tip, which can be clamped with the suction tip and sealed. It has a simple structure and is easy to use. The through-hole diameter of the separation plate is smaller than the diameter of the suction tip. It is controlled by an electromagnet. When power is turned on, the separation plate is adsorbed on the electromagnet to push the suction tip out of the trachea interface to achieve separation. The utility model automatically controls the position of the X-axis linear module and the Z-axis linear module to seal the trachea interface and the suction tip. After the suction, transfer and discharge operations, the discarded suction tips are separated by the separation parts. The simple structure reduces production costs and the control is convenient and reasonable. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The present invention can be further described by way of non-limiting examples given in the accompanying drawings;

[0017] Figure 1 This is a schematic diagram of the structure of an NGS automated pipetting analysis device in this utility model. Figure 1 ;

[0018] Figure 2 This is a schematic diagram of the structure of an NGS automated pipetting analysis device in this utility model. Figure 2 ;

[0019] Figure 3 for Figure 2 A schematic diagram of the structure at point A in the middle;

[0020] Figure 4 This is a schematic structural diagram of the pipette of the utility model;

[0021] The main component symbols are described as follows:

[0022] Bracket 1, X-axis linear module 2, Z-axis linear module 3, storage rack 4, trash box 41, tip box 42, sampling box 43, placement box 44, positioning seat 45, support plate 5, electric cylinder 51, pipette 52, cylinder 521, connecting box 522, air pipe interface 523, rubber ring 524, solenoid valve 525, separator 53, separation plate 531, electromagnet 532, limit block 533 DETAILED DESCRIPTION

[0023] In order to enable those skilled in the art to better understand the present invention, the technical solution of the present invention is further described below with reference to the accompanying drawings and embodiments.

[0024] like Figure 1-4 As shown, an NGS automated pipetting analysis device of the present invention includes a bracket 1, an X-axis linear module 2, a Z-axis linear module 3 and a storage rack 4. The top of the bracket 1 is fixedly connected to the X-axis linear module 2, the mobile end of the X-axis linear module 2 is fixedly connected to the Z-axis linear module 3, and the mobile end of the Z-axis linear module 3 is connected to a support plate 5;

[0025] The bracket 1 is used to support other components. The X-axis linear module 2 is fixed to the top of the bracket 1. The X-axis linear module 2 can control the lateral movement of the pipette 52. Multiple position sensors can be set on the X-axis linear module 2 to more accurately locate the positions of the garbage box 41, the tip box 42, the sampling box 43 and the placement box 44. The Z-axis linear module 3 controls the longitudinal movement of the pipette 52. The Z-axis linear module 3 can be used to insert the trachea interface 523 into the tip fixed in the tip box 42 so that it is tightly connected through the rubber ring 524. The Z-axis linear module 3 can be provided with a position sensor to more accurately locate the height of the connection between the trachea interface 523 and the tip. The support plate 5 is used to provide support and installation platform for components such as the pipette 52.

[0026] The rack 4 is provided with a trash box 41 , a pipette tip box 42 , a sampling box 43 and a sample placement box 44 . The pipette tip box 42 is provided with pipette tips; the sampling box 43 is provided with sample liquid for gene treatment.

[0027] The support plate 5 is fixedly connected to the electric cylinder 51, the pipette 52 and the separator 53. The pipette 52 is provided with an air cylinder 521 connected to the electric cylinder 51. The air pipe of the cylinder 521 is connected to the connecting box 522. The connecting box 522 is connected to multiple air pipe interfaces 523. The ends of all air pipe interfaces 523 are provided with rubber rings 524. The outer wall of the rubber ring 524 is a plug shape that matches the suction head. The separator 53 includes a separation plate 531. The separation plate 531 is provided with a through hole that matches the air pipe interface 523. The diameter of the through hole is smaller than the diameter of the suction head. An electromagnet 532 is provided on the lower side of the separation plate 531. The electromagnet 532 is fixedly connected to the support plate 5.

[0028] The electric cylinder 51 is energized to control its extension and retraction. The servo electric cylinder 51 can be used to precisely control the extension and retraction length, thereby controlling the suction and discharge amount of the suction head. The air cylinder 521 is actuated by the electric cylinder 51 to inhale or discharge gas, thereby controlling the suction and discharge of the suction head. The connecting box 522 connects the air pipe interface 523 with the air cylinder 521, and all installed suction heads are uniformly controlled by the air cylinder 521. The outer wall of the rubber ring 524 is in the shape of a plug that matches the suction head, which can both snap into place with the suction head and provide a seal. The structure is simple and easy to use. The diameter of the through hole of the separation plate 531 is smaller than the diameter of the suction head. It is controlled by the electromagnet 532. When energized, the separation plate 531 is adsorbed on the electromagnet 532, thereby pressing the suction head against the air pipe interface 523 to achieve separation. Upwardly extending L-plates are provided on both sides of the separation plate 531. The electromagnet 532 cooperates with the L-plates, which not only raises the height of the electromagnet 532 to avoid interference but also reduces the material used in the support plate 5.

[0029] Furthermore, a solenoid valve 525 is provided between the connecting box 522 and each air pipe interface 523. The solenoid valve 525 is used to control the opening and closing of each air pipe interface 523. While the suction heads are uniformly controlled by the cylinder 521, the solenoid valve 525 cuts off the connection of some air pipe interfaces 523 to prevent air leakage. This allows only some suction heads to be installed as needed, providing more comprehensive functions.

[0030] Further defined, the rack 4 is provided with a positioning seat 45, and the tip box 42, sampling box 43, and layout box 44 are detachably fixedly connected above the positioning seat 45. The positioning seat 45 serves to fix the position and height of the tip box 42, sampling box 43, and layout box 44, facilitating the pre-setting of the coordinates of the X-axis linear module 2 and the Z-axis linear module 3 to achieve automated control. It also raises the tip box 42, sampling box 43, and layout box 44, allowing the trash box 41 to have a large capacity while being lowered relative to the tip box 42. The rack 4 can secure the tip box 42, sampling box 43, and layout box 44 using pins or bolts.

[0031] Further defined, the number of electromagnets 532 is two, and the two electromagnets 532 are respectively arranged on both sides of the separation plate 531. In this way, the separation plate 531 is evenly stressed, and it is not easy to get stuck with the trachea interface 523 when rising, thereby damaging the interface.

[0032] Furthermore, a limit block 533 is provided on either side of the separation plate 531, and the limit blocks 533 are fixedly connected to the support plate 5. The limit blocks 533 prevent the separation plate 531 from moving upward excessively and causing damage. Alternatively, a second limit block may be provided to limit horizontal movement of the separation plate 531 to prevent lateral damage to the tracheal interface 523, depending on practical circumstances.

[0033] It is further defined that the tracheal interface 523 is detachably connected to the outer wall of the pipette 52, so as to facilitate installation and replacement of the tracheal interface 523.

[0034] The utility model automatically controls the positions of the X-axis linear module 2 and the Z-axis linear module 3 to seal the air pipe interface 523 and the suction tip. After the suction, transfer and discharge operations, the discarded suction tips are separated by the separation member 53. The structure is simple, the production cost is reduced, and the control is convenient and reasonable.

[0035] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by persons skilled in the art without departing from the spirit and technical principles disclosed herein shall be covered by the claims of the present invention.

Claims

1. An NGS automated liquid handling analysis device, comprising a support (1), an X-axis linear module (2), a Z-axis linear module (3) and a storage rack (4), characterized in that: The top of the bracket (1) is fixedly connected to the X-axis linear module (2), the mobile end of the X-axis linear module (2) is fixedly connected to the Z-axis linear module (3), and the mobile end of the Z-axis linear module (3) is connected to a support plate (5); The storage rack (4) is provided with a garbage box (41), a suction tip box (42), a sampling box (43) and a sample placement box (44), wherein the suction tip box (42) is provided with suction tips; The support plate (5) is fixedly connected with an electric cylinder (51), a pipette (52) and a separator (53); an air cylinder (521) connected to the electric cylinder (51) is provided inside the pipette (52); an air pipe of the air cylinder (521) is connected to a connecting box (522); the connecting box (522) is connected to a plurality of air pipe interfaces (523); rubber rings (524) are provided at the ends of all the air pipe interfaces (523); the outer wall of the rubber ring (524) is in the shape of a plug that matches the suction head; the separator (53) comprises a separation plate (531); the separation plate (531) is provided with a through hole that matches the air pipe interface (523); the diameter of the through hole is smaller than the diameter of the suction head; an electromagnet (532) is provided on the lower side of the separation plate (531); the electromagnet (532) is fixedly connected to the support plate (5).

2. The NGS automated liquid handling analysis device according to claim 1, characterized in that: A solenoid valve (525) is provided between the communication box (522) and each air pipe interface (523).

3. The NGS automated liquid handling analysis device according to claim 1, characterized in that: The storage rack (4) is provided with a positioning seat (45), and the suction head box (42), the sampling box (43) and the placement box (44) are detachably fixedly connected above the positioning seat (45).

4. The NGS automated liquid handling analysis device according to claim 1, characterized in that: There are two electromagnets (532), and the two electromagnets (532) are respectively arranged on both sides of the separation plate (531).

5. The NGS automated liquid handling analysis device according to claim 1, characterized in that: A limit block (533) is provided on both sides above the separation plate (531), and the limit block (533) is fixedly connected to the support plate (5).

6. The NGS automated liquid handling analysis device according to claim 1, characterized in that: The trachea interface (523) is detachably connected to the outer wall of the pipette (52).

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