Tumor cell scratching device

Abstract

The utility model discloses a tumor cell scratching device, which relates to the technical field of cell experiments and comprises a worktable body, a protective baffle is fixedly mounted at the center of the upper surface of the worktable body, and a scratching component is arranged above the protective baffle. The motor is started, the output end of the motor drives the first gear to rotate, the first gear drives the double-face circular gear ring meshed with the first gear to rotate, and therefore the four second gears meshed with the inner side teeth of the double-face circular gear ring are driven to rotate; when each group of second gears rotate, a correspondingly meshed rack is driven to move in the worktable main body, and when the rack moves, a fixed rod fixed at the top of the rack is driven to synchronously move towards the direction of the culture plate, so that a telescopic rod and an L-shaped positioning block are driven to move towards the direction of the culture plate, and on one hand, the culture plate is quickly centered and accurately positioned; on the other hand, the culture plates with different sizes can be positioned, and the practicability of the device is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell experiments, in particular to a tumor cell scratching device. Background Art

[0002] Tumor cells multiply in the human body and can cause cancer. By scratching tumor cells and observing their migration, we can understand the metastasis process of cancer and identify new therapeutic targets through analysis, helping doctors more accurately predict disease progression and survival rates in patients. Understanding the mechanisms of tumor cell migration can also help researchers screen and develop more effective anti-cancer drugs. The current method for cell scratch experiments is: ① Use a marker pen to draw a horizontal line on the back of a 6-well plate; ② Inoculate cells evenly in the 6-well plate; ③ After the cells have spread over the 6-well plate, use a 200-μl pipette tip to create cell scratches perpendicular to the well plate; ④ Aspirate the cell culture medium and rinse the plate three times with PBS to wash away the cell debris produced by the scratch; ⑤ Add serum-free culture medium and take photos to record.

[0003] An existing patent (publication number: CN218465783U) discloses a cell scratching device, which is equipped with a scratching mechanism. By sliding the first sliding plate, the longitudinal position of the scratch can be adjusted. By rotating the fixing ring, the fixing ring can drive the threaded rod to rotate. During the rotation of the threaded rod, the second sliding plate can be driven to move, thereby adjusting the lateral position of the scratch, thereby realizing the determination of the scratch position. The operation is simple and easy to use. Through the positioning mechanism, under the action of the elastic force of the spring, the four L-shaped plates can be tightly attached to the four corners of the six-hole plate, thereby positioning the six-hole plate and avoiding the phenomenon of offset of the six-hole plate.

[0004] However, the above technical solution still has certain defects. The positioning mechanism of the above device uses four sets of springs to position the four corners of the six-hole plate. Therefore, the required spring stiffness coefficient must be large to ensure that the six-hole plate is firmly positioned. This results in the need to press the four sets of springs when placing the six-hole plate, which is time-consuming and labor-intensive. At the same time, the positioning mechanism can only be adapted to six-hole plates of relatively fixed sizes, and has certain limitations in actual use. For this reason, a tumor cell scratching device is proposed. Utility Model Content

[0005] Based on this, the purpose of the present invention is to provide a tumor cell scratching device to solve the technical problems raised in the above background.

[0006] To achieve the above-mentioned object, the present invention provides the following technical solution: a tumor cell scratching device, comprising a workbench body, a protective baffle fixedly mounted at the center of the upper surface of the workbench body, a scratching assembly disposed above the protective baffle, and a positioning assembly mounted inside the workbench body;

[0007] The positioning assembly includes an annular groove opened in the workbench main body, a double-sided circular gear ring is connected to the annular groove through a bearing, the inner teeth of the double-sided circular gear ring are meshed with four second gears, the four second gears are rotatably connected to the bottom of the annular groove through a rotating shaft, and the upper end teeth of the four second gears are meshed with racks, each group of the racks are slidably connected to the inside of the workbench main body, and the upper surface of each group of racks is fixedly connected to a fixing rod, the top end of each group of the fixing rods extends through and extends above the workbench main body, and a telescopic rod is fixedly connected to one side of the top end of the fixing rod, the telescopic end of the telescopic rod is fixedly connected to an L-shaped positioning block, a spring is fixedly connected between the fixing rod and the L-shaped positioning block, and the spring is sleeved on the outer wall of the telescopic rod.

[0008] As a preferred technical solution of a tumor cell scratching device of the present invention, a motor is fixedly installed in the internal cavity on one side of the workbench main body, and the output end of the motor is fixedly connected to a first gear. One side of the first gear is engaged with the outer teeth of the double-sided circular gear ring, and an inspection door is installed outside the cavity where the motor is installed inside one side of the workbench main body.

[0009] As an optimal technical solution for a tumor cell scratching device of the present invention, a movable slide groove matching the four racks is provided in the workbench body, each rack is slidably connected to the corresponding movable slide groove, and a yield groove matching the fixed rod is provided on the upper surface of the workbench body.

[0010] As a preferred technical solution of a tumor cell scratching device of the present invention, the scratching assembly includes support columns fixedly installed at the four corners of the upper surface of the workbench body, a sliding rod fixedly installed between each pair of the support columns on the same side, and the two groups of sliding rods are slidably connected to the same movable plate, fixed blocks are fixedly installed on both sides of the bottom end of the movable plate, a threaded rod is fixedly connected between the two fixed blocks, a slider is threadedly connected to the outer wall of the threaded rod, a connecting plate is fixedly installed at the bottom of the slider, a hydraulic rod is fixedly installed at the bottom of the connecting plate, a scratching pen is fixedly installed at the output end of the hydraulic rod, and one end of the threaded rod extends through to the outside of a fixed block and is fixedly installed with a turntable.

[0011] As a preferred technical solution of the tumor cell scratching device of the present invention, a guide block is fixedly installed on the top of the slider, a guide groove matching the guide block is opened at the bottom of the movable plate, and the guide block is slidably connected to the guide groove.

[0012] As a preferred technical solution of the tumor cell scratching device of the present invention, four groups of L-shaped pads are fixedly installed on the upper surface of the workbench body inside the protective baffle, and the same culture plate is placed on the upper surface of the four groups of L-shaped pads.

[0013] As a preferred technical solution of the tumor cell scratching device of the present invention, the upper surface of the culture plate is provided with a scale.

[0014] In summary, the present invention has the following beneficial effects:

[0015] The utility model starts the motor, and the output end of the motor drives the first gear to rotate, and the first gear drives the double-sided circular gear ring meshed with it to rotate, thereby driving the four second gears meshed with the inner teeth of the double-sided circular gear ring to rotate. When each group of second gears rotates, it drives the corresponding meshed rack to move in the workbench body. When the rack moves, it drives the fixed rod fixed on the top of it to move synchronously toward the culture plate, thereby driving the telescopic rod and the L-shaped positioning block to move toward the culture plate. On the one hand, the culture plate can be quickly and accurately positioned in the center, and on the other hand, it can also achieve the positioning of culture plates of different sizes, thereby improving the practicality of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0017] Figure 2 This is a schematic diagram of the partial structure of the scratching component of the present utility model;

[0018] Figure 3 This is a schematic diagram of the internal structure of the protective baffle of the present utility model;

[0019] Figure 4 This is a schematic diagram of the internal structure of the workbench body of the utility model;

[0020] Figure 5 For the utility model Figure 3 Enlarged view of point A in the middle;

[0021] Figure 6 This is a schematic diagram of the double-sided circular gear ring structure of the present utility model.

[0022] In the figure: 100, workbench body; 200, scratching assembly; 300, positioning assembly;

[0023] 110, protective baffle; 120, L-shaped pad; 130, culture plate; 140, scale; 150, maintenance door;

[0024] 210, support column; 220, slide bar; 230, movable plate; 231, guide groove; 240, fixed block; 250, threaded rod; 260, slider; 270, connecting plate; 280, hydraulic rod; 290, scratch pen; 2910, turntable;

[0025] 310, annular groove; 320, double-sided circular gear ring; 330, motor; 340, first gear; 350, second gear; 360, rack; 370, movable slide; 380, fixed rod; 390, telescopic rod; 3910, L-shaped positioning block; 3920, spring; 3930, clearance groove. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. The embodiments described below with reference to the drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0027] The following describes an embodiment of the present invention based on its overall structure.

[0028] A tumor cell scratching device, such as Figure 1-6 As shown, it includes a workbench main body 100, a protective baffle 110 is fixedly installed at the center of the upper surface of the workbench main body 100, a scratching assembly 200 is provided above the protective baffle 110, and a positioning assembly 300 is installed inside the workbench main body 100; the positioning assembly 300 includes an annular groove 310 opened in the workbench main body 100, a double-sided circular gear ring 320 is connected to the annular groove 310 through a bearing, and the inner teeth of the double-sided circular gear ring 320 are meshed with four second gears 350, and the four second gears 350 are rotatably connected to the bottom of the annular groove 310 through a rotating shaft, and the upper end teeth of the four second gears 350 are meshed with racks 360, and each set of racks 360 are slidably connected to the inside of the workbench main body 100, and the upper surface of each set of racks 360 is It is fixedly connected with a fixing rod 380, and the top of each group of fixing rods 380 extends through the top of the workbench body 100, and a telescopic rod 390 is fixedly connected to one side of the top of the fixing rod 380, and the telescopic end of the telescopic rod 390 is fixedly connected with an L-shaped positioning block 3910, and a spring 3920 is fixedly connected between the fixing rod 380 and the L-shaped positioning block 3910, and the spring 3920 is sleeved on the outer wall of the telescopic rod 390. A motor 330 is fixedly installed in the internal cavity of one side of the workbench body 100, and the output end of the motor 330 is fixedly connected to the first gear 340, and one side of the first gear 340 is engaged with the outer teeth of the double-sided circular gear ring 320. An inspection door 150 is installed outside the cavity where the motor 330 is installed inside one side of the workbench body 100.

[0029] The culture plate 130 is placed on the L-shaped pad 120, and the motor 330 is started by the controller. The output end of the motor 330 drives the first gear 340 to rotate. The first gear 340 drives the double-sided circular gear ring 320 meshing with it to rotate, thereby driving the four second gears 350 meshing with the inner teeth of the double-sided circular gear ring 320 to rotate. When each group of second gears 350 rotates, it drives the corresponding meshing rack 360 to move in the workbench body 100. When the rack 360 moves, it drives the fixed rod 380 fixed on its top to move synchronously toward the culture plate 130, thereby driving the telescopic rod 390 and the L-shaped positioning block 3910 to move toward the culture plate 130, accurately positioning and fixing the culture plate 130 in the center. The spring 3920 sleeved on the outer wall of the telescopic rod 390 plays a buffering and protective role when clamping and positioning the culture plate 130. At the same time, the positioning assembly 300 also realizes the positioning of culture plates 130 of different sizes, thereby improving the practicality of the device.

[0030] Please refer to Figure 3 and Figure 4 As shown, a movable slide 370 matching the four racks 360 is opened in the workbench body 100, each rack 360 is slidably connected to the corresponding movable slide 370, and a yield groove 3930 matching the fixed rod 380 is opened on the upper surface of the workbench body 100.

[0031] The movable slide groove 370 is provided so that the rack 360 can slide stably in the movable slide groove 370 . Meanwhile, the clearance groove 3930 is provided to provide a moving space for the fixing rod 380 to move on the upper surface of the workbench body 100 .

[0032] Please refer to Figure 1 and Figure 2 As shown, the scratching assembly 200 includes support columns 210 fixedly mounted at the four corners of the upper surface of the workbench body 100, a sliding rod 220 fixedly mounted between each pair of support columns 210 on the same side, and the same movable plate 230 is slidably connected to the two sets of sliding rods 220, fixed blocks 240 are fixedly mounted on both sides of the bottom end of the movable plate 230, a threaded rod 250 is fixedly connected between the two fixed blocks 240, a slider 260 is threadedly connected to the outer wall of the threaded rod 250, a connecting plate 270 is fixedly mounted on the bottom of the slider 260, a hydraulic rod 280 is fixedly mounted on the bottom of the connecting plate 270, a scratching pen 290 is fixedly mounted on the output end of the hydraulic rod 280, one end of the threaded rod 250 extends through and extends to the outside of a fixed block 240 and is fixedly mounted with a turntable 2910, a guide block is fixedly mounted on the top of the slider 260, a guide groove 231 matching the guide block is provided at the bottom of the movable plate 230, and the guide block is slidably connected to the guide groove 231.

[0033] After the culture plate 130 is positioned by the positioning assembly 300, the hydraulic rod 280 is started to make the scratch pen 290 close to the surface of the culture plate 130, and the movable plate 230 is pushed to move on the slide rod 220 to adjust the longitudinal position of the scratch. Then, by rotating the turntable 2910, the threaded rod 250 is driven to rotate, thereby driving the slider 260 threadedly connected to it to move stably laterally with the cooperation of the guide block and the guide groove 231, thereby adjusting the lateral position of the scratch, realizing the determination of the scratch position. The scratch assembly 200 is simple to operate and convenient for operators to use.

[0034] Please refer to Figure 1-3 As shown, four groups of L-shaped pads 120 are fixedly installed on the upper surface of the workbench body 100 in the protective baffle 110 , and the same culture plate 130 is placed on the upper surface of the four groups of L-shaped pads 120 , and a scale 140 is opened on the upper surface of the culture plate 130 .

[0035] By installing four sets of L-shaped pads 120, the culture plate 130 is raised to a certain height, and the scratching assembly 200 cooperates with the scale 140 on the upper surface of the culture plate 130 to accurately record the scratch distance.

[0036] When in use, the culture plate 130 is placed on the L-shaped pad 120, and the motor 330 is started by the controller. The output end of the motor 330 drives the first gear 340 to rotate, and the first gear 340 drives the double-sided circular gear ring 320 meshing with it to rotate, thereby driving the four second gears 350 meshing with the inner teeth of the double-sided circular gear ring 320 to rotate. When each group of second gears 350 rotates, it drives the corresponding meshing rack 360 to move in the workbench body 100, and when the rack 360 moves, it drives the fixed rod 380 fixed on its top to rotate simultaneously. The telescopic rod 390 moves toward the culture plate 130, thereby driving the telescopic rod 390 and the L-shaped positioning block 3910 to move toward the culture plate 130, accurately positioning and fixing the culture plate 130 in the center, and the spring 3920 sleeved on the outer wall of the telescopic rod 390 plays a buffering and protective role when clamping and positioning the culture plate 130. At the same time, the positioning component 300 also realizes the positioning of culture plates 130 of different sizes, thereby improving the practicality of the device. The parts not involved in the device are the same as the existing technology or can be realized by using the existing technology.

[0037] Although embodiments of the present invention have been shown and described, these specific embodiments are merely explanations of the present invention and are not limitations on the present invention. The specific features, structures, materials, or characteristics described may be combined in a suitable manner in any one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions, and variations to the embodiments as needed without departing from the principles and purpose of the present invention, but as long as they are within the scope of the claims of the present invention, they are protected by patent law.

Claims

1. A tumor cell scratching device, comprising a workbench body (100), characterized in that: A protective baffle (110) is fixedly installed at the center of the upper surface of the workbench body (100), a scratching assembly (200) is provided above the protective baffle (110), and a positioning assembly (300) is installed inside the workbench body (100); The positioning assembly (300) includes an annular groove (310) provided in the workbench body (100), a double-sided circular gear ring (320) is connected to the annular groove (310) via a bearing, four second gears (350) are meshed with the inner teeth of the double-sided circular gear ring (320), the four second gears (350) are rotatably connected to the bottom of the annular groove (310) via a rotating shaft, the upper end teeth of the four second gears (350) are meshed with racks (360), and each set of the racks (360) is meshed with the inner teeth of the workbench body (100). The upper surface of each set of racks (360) is fixedly connected with a fixed rod (380), the top of each set of fixed rods (380) extends through and extends above the workbench body (100), and one side of the top of the fixed rod (380) is fixedly connected with a telescopic rod (390), the telescopic end of the telescopic rod (390) is fixedly connected with an L-shaped positioning block (3910), and a spring (3920) is fixedly connected between the fixed rod (380) and the L-shaped positioning block (3910), and the spring (3920) is sleeved on the outer wall of the telescopic rod (390).

2. The tumor cell scratching device according to claim 1, characterized in that: A motor (330) is fixedly installed in an internal cavity on one side of the workbench body (100); an output end of the motor (330) is fixedly connected to a first gear (340); one side of the first gear (340) is meshed with outer teeth of a double-sided circular gear ring (320); and an inspection door (150) is installed outside the cavity on one side of the workbench body (100) in which the motor (330) is installed.

3. The tumor cell scratching device according to claim 1, characterized in that: The workbench body (100) is provided with a movable slide groove (370) matching the four racks (360), each rack (360) is slidably connected to the corresponding movable slide groove (370), and the upper surface of the workbench body (100) is provided with a clearance groove (3930) matching the fixed rod (380).

4. The tumor cell scratching device according to claim 1, characterized in that: The scratching assembly (200) includes support columns (210) fixedly installed at the four corners of the upper surface of the workbench body (100), a sliding rod (220) fixedly installed between each pair of the support columns (210) located on the same side, and the same movable plate (230) is slidably connected to the two groups of the sliding rods (220), and fixed blocks (240) are fixedly installed on both sides of the bottom end of the movable plate (230). A threaded rod (250) is fixedly connected between the two fixed blocks (240), and the outer wall of the threaded rod (250) is threadedly connected to a slider (260), and a connecting plate (270) is fixedly installed at the bottom of the slider (260), and a hydraulic rod (280) is fixedly installed at the bottom of the connecting plate (270), and a scratching pen (290) is fixedly installed at the output end of the hydraulic rod (280), and one end of the threaded rod (250) extends through and extends to the outside of a fixed block (240) and is fixedly installed with a turntable (2910).

5. The tumor cell scratching device according to claim 4, characterized in that: A guide block is fixedly mounted on the top of the slider (260), and a guide groove (231) matching the guide block is provided on the bottom of the movable plate (230), and the guide block is slidably connected to the guide groove (231).

6. The tumor cell scratching device according to claim 1, characterized in that: Four groups of L-shaped pads (120) are fixedly installed on the upper surface of the workbench body (100) inside the protective baffle (110), and the same culture plate (130) is placed on the upper surface of the four groups of L-shaped pads (120).

7. The tumor cell scratching device according to claim 6, characterized in that: The upper surface of the culture plate (130) is provided with a scale (140).

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