Cell co-culture device

Abstract

The utility model discloses a cell co-culture device and relates to the technical field of cell culture. The lower inner wall of the culture box is fixedly connected with a placement seat, the inner wall of the placement seat is movably clamped with a culture box body, the inner wall of the culture box is provided with a fixing mechanism matched with the culture box body for use, the lower inner wall of the culture box is provided with a vibration mechanism matched with the placement seat for use, and the vibration mechanism comprises a support plate; the bottom end of the support plate is fixedly connected with the lower inner wall of the culture box, and the inner wall of one side of the support plate is rotationally connected with two long rods. Through the arrangement of the vibration mechanism structure, a dynamic culture environment can be provided for cells, interaction of the cells in vivo is simulated more truly, and then the cell co-culture effect is improved; a culture box containing cells can be conveniently and stably fixed, and waste of co-culture cells caused by toppling of the culture box is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell co-culture device. Background Art

[0002] In cell biology research, in order to more accurately study the physiological and pathological processes of cells and the interactions between cells, it is necessary to establish a culture system that is closer to the in vivo environment. Monolayer cell culture technology cannot fully reflect the complex microenvironment faced by cells in the body and the mutual influence between cells. Therefore, cell co-culture technology came into being in order to simulate the in vivo microenvironment to the greatest extent possible and better observe the interactions between cells and cells, and cells and the culture environment.

[0003] First, when cells are co-cultured in direct contact, the degree of cell mixing and distribution is uneven, resulting in differences in cell interactions and, therefore, an inability to achieve the best co-culture effect. Second, during co-culture, the culture box is generally placed directly on a support in the incubator. This placement method can easily cause the culture box to tip over, resulting in the spillage and waste of co-cultured cells. Utility Model Content

[0004] In order to solve the problems of uneven mixing and distribution of cells in existing co-culture devices and unstable placement of culture boxes during co-culture, the purpose of the utility model is to provide a cell co-culture device.

[0005] In order to solve the above technical problems, the present invention adopts the following technical solutions: a cell co-culture device, comprising an incubator, a lid hingedly connected to one side of the top of the incubator, and a mounting groove provided on the lower inner wall of the lid, an ultraviolet disinfection lamp fixedly connected to the inner wall of the mounting groove, the arrangement of the ultraviolet disinfection lamp facilitates providing a sterile environment in the incubator, a placement seat fixedly connected to the lower inner wall of the incubator, a culture box body movably connected to the inner wall of the placement seat, the culture box body is convenient for holding cells to be cultured, a through groove is provided at one end of the incubator, the inner wall of the through groove is fixedly connected to the inner wall of the The incubator is fixedly connected with a transparent glass body, which serves to facilitate observation of the culture conditions of cells in the culture box. The inner wall of the incubator is provided with a fixing mechanism used in conjunction with the incubator body, and the fixing mechanism serves to facilitate the stable fixation of the incubator. The lower inner wall of the incubator is provided with a vibration mechanism used in conjunction with the placement seat, and the vibration mechanism serves to facilitate providing a dynamic culture environment for the cells, more realistically simulating the interaction of cells in the body. The vibration mechanism includes a bracket plate, the bottom end of the bracket plate is fixedly connected to the lower inner wall of the incubator, and one side inner wall of the bracket plate is rotatably connected to two long rods. The lower part of one end of the bracket plate is fixedly connected to a support plate, and the top of the support plate is fixedly connected to a motor, and the support plate serves to support the motor. The output end of the motor passes through one end of the bracket plate and is fixedly connected to one end of one of the long rods, so that the output end of the motor drives one of the long rods to rotate. The outer surfaces of the two long rods are fixedly sleeved with sprockets, and the outer surfaces of the two sprockets are commonly meshed and connected with a chain. The sprocket cooperates with the chain to facilitate the synchronous rotation of the two long rods. One end of the two long rods is fixedly connected to a circular plate, and the rotation of the two long rods drives the two The circular plates rotate synchronously, and one end of the two circular plates is hinged with a connecting rod through a rotating shaft, one end of the two connecting rods is hinged with a connecting seat through a rotating shaft, and the top ends of the two connecting seats are fixedly connected to a moving rod. The two circular plates rotate in conjunction with the connecting rod and the connecting seat to make the two moving rods move up and down synchronously. The top ends of the two moving rods pass through the upper inner wall of the bracket plate and are fixedly connected to a top plate used in conjunction with the placement seat. The movement of the two moving rods drives the two top plates to continuously knock on the bottom end of the placement seat, providing a dynamic culture environment for cells and more realistically simulating the interaction of cells in the body.

[0006] The top end of the lifting post is fixedly mounted on the lifting post, and the bottom end of the lifting post is fixedly mounted on the lifting post.

[0007] Compared with the prior art, the beneficial effects of the present invention are:

[0008] 1. This application provides a dynamic culture environment for cells by setting up a vibration mechanism structure, more realistically simulating the interaction of cells in the body, thereby improving the effect of cell co-culture;

[0009] 2. The present application provides a fixing mechanism structure, which can facilitate the stable fixation of the culture box containing cells, thereby preventing the culture box from tipping over and causing waste of co-cultured cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0011] Figure 1 This is a schematic structural diagram of the utility model.

[0012] Figure 2 This is a schematic diagram of the cross-sectional structure of the utility model.

[0013] Figure 3 This is a schematic diagram of the cross-sectional structure of the vibration mechanism of the utility model.

[0014] Figure 4 This is a schematic cross-sectional structural diagram of the vibration mechanism of the present invention from another perspective.

[0015] Figure 5 This is a schematic diagram of the cross-sectional structure of the fixing mechanism of the present invention.

[0016] In the figure: 1. incubator; 2. vibration mechanism; 21. bracket plate; 22. chain; 23. sprocket; 24. long rod; 25. guide plate; 26. moving rod; 27. connecting rod; 28. circular plate; 29. support plate; 201. motor; 202. connecting seat; 203. top plate; 3. fixing mechanism; 31. second fixing plate; 32. positioning rod; 33. mounting seat; 34. vertical plate; 35. telescopic gas rod; 36. vertical rod; 37. triangle plate; 38. first cross bar; 39. first fixing plate; 301. anti-slip pad; 302. second cross bar; 4. mounting slot; 5. perspective glass; 6. through slot; 7. box cover; 8. ultraviolet disinfection lamp; 9. incubator body; 10. placement seat. DETAILED DESCRIPTION

[0017] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0018] Example: Figure 1-5As shown, the utility model provides a cell co-culture device, including an incubator 1, the lower inner wall of the incubator 1 is fixedly connected to a placement seat 10, the inner wall of the placement seat 10 is movably connected to a culture box body 9, the inner wall of the incubator 1 is provided with a fixing mechanism 3 used in conjunction with the culture box body 9, the lower inner wall of the incubator 1 is provided with a vibration mechanism 2 used in conjunction with the placement seat 10, the vibration mechanism 2 includes a bracket plate 21, the bottom end of the bracket plate 21 is fixedly connected to the lower inner wall of the incubator 1, and the bracket plate The inner wall of one side of 21 is rotatably connected to two long rods 24, and the lower part of one end of the bracket plate 21 is fixedly connected to the support plate 29, and the top of the support plate 29 is fixedly connected to the motor 201, and the output end of the motor 201 passes through one end of the bracket plate 21 and is fixedly connected to one end of one of the long rods 24. The outer surfaces of the two long rods 24 are fixedly sleeved with sprockets 23, and the outer surfaces of the two sprockets 23 are jointly engaged with the chain 22. One end of the two long rods 24 is fixedly connected to a circular plate 28, and the two circular plates are fixedly connected to the outer surfaces of the two sprockets 23. One end of the plate 28 is hinged with a connecting rod 27 through a rotating shaft, and one end of the two connecting rods 27 is hinged with a connecting seat 202 through a rotating shaft, and the top of the two connecting seats 202 is fixedly connected with a moving rod 26. The tops of the two moving rods 26 pass through the upper inner wall of the bracket plate 21 and are fixedly connected with a top plate 203 used in conjunction with the placement seat 10. The cells to be co-cultured are placed through the culture box body 9, and then the culture box body 9 is firmly fixed by the fixing mechanism 3. The vibration mechanism 2 is used to facilitate the cell A dynamic culture environment is provided to more realistically simulate the interaction of cells in the body. The long rod 24 is driven to rotate by the motor 201, and the two long rods 24 are driven by the sprocket 23 and the chain 22 to rotate synchronously. The circular plate 28, connecting rod 27, connecting seat 202 and moving rod 26 on the long rod 24 cooperate to drive the top plate 203 to move up and down, thereby causing the culture box body 9 on the placement seat 10 to vibrate. This vibration can simulate the dynamic environment of cells in the body, promote cell growth and metabolism, and improve the effect of cell culture.

[0019] The fixing mechanism 3 includes a vertical plate 34, the bottom end of which is fixedly connected to the lower inner wall of the incubator 1, one end of the vertical plate 34 is fixedly connected to a mounting seat 33, the lower inner wall of the mounting seat 33 is fixedly connected to a telescopic gas rod 35, and the output end of the telescopic gas rod 35 is fixedly connected to a vertical rod 36, the upper end of one end of the vertical rod 36 is hinged to a triangular plate 37 through a rotating shaft, one end of the vertical plate 34 is fixedly connected to a positioning rod 32, and the triangular plate 37 is rotatably sleeved on the outer surface of the positioning rod 32, the upper end of one end of the triangular plate 37 is hinged to a first cross bar 38 through a rotating shaft, and the first cross bar 38 is hinged to the upper end of the triangular plate 37. One end is fixedly connected to a first fixed plate 39, and a second cross bar 302 is fixedly connected to an inner wall of one side of the incubator 1, and one end of the second cross bar 302 is fixedly connected to a second fixed plate 31 used in conjunction with the first fixed plate 39. The telescopic gas rod 35 pushes the vertical rod 36 to move up and down, and the vertical rod 36 drives the triangular plate 37 to rotate around the positioning rod 32. The triangular plate 37 then pushes the first fixed plate 39 to move through the first cross bar 38, and cooperates with the second fixed plate 31 to fix the culture box body 9. This ensures that the culture box body 9 will not shake or displace during the culture process, thereby avoiding the scattering of co-cultured cells.

[0020] A box cover 7 is hinged on one side of the top of the incubator 1, and a mounting groove 4 is provided on the lower inner wall of the box cover 7. An ultraviolet disinfection lamp 8 is fixedly connected to the inner wall of the mounting groove 4. The ultraviolet disinfection lamp 8 is provided on the box cover 7, which can disinfect the interior of the incubator 1 when it is closed, effectively killing bacteria and viruses, and providing a sterile environment for the cell culture process.

[0021] A through slot 6 is provided at one end of the incubator 1 , and a transparent glass body 5 is fixedly connected to the inner wall of the through slot 6 . The transparent glass body 5 allows researchers to intuitively understand the morphology, quantity and growth status of cells, providing convenience for experimental research.

[0022] The tops of the two top plates 203 are flush with each other, which can ensure that the placement seat 10 is evenly stressed, avoid tilting or shifting of the culture box body 9 during vibration, and ensure the stability of cell culture.

[0023] A guide plate 25 is fixedly connected to the inner wall of one side of the bracket plate 21 for use with the two moving rods 26, and the top ends of the two moving rods 26 pass through the bottom ends of the guide plate 25. The guide plate 25 can guide the movement of the moving rods 26, ensuring that the moving rods 26 maintain vertical movement during the up and down movement to avoid deviation or shaking.

[0024] The inner wall of the first crossbar 38 is slidably connected to the top of the vertical plate 34, which can ensure the stability and linearity of the first crossbar 38 during movement and avoid bending or twisting.

[0025] The inner walls of the first fixing plate 39 and the second fixing plate 31 are fixedly connected with anti-slip pads 301, and the inner walls of the two anti-slip pads 301 are arc-shaped. The two anti-slip pads 301 are distributed in a mirror image. The anti-slip pads 301 can increase the friction between the first fixing plate 39 and the second fixing plate 31 and the culture box body 9 to prevent the culture box from sliding or shifting during the fixation process. The arc-shaped anti-slip pads 301 can better fit the outer surface of the culture box body 9 and improve the fixing effect. The mirror-distributed anti-slip pads 301 can make the fixation more uniform.

[0026] Working principle: First, the cells to be cultured are placed inside the culture box body 9, then the lid is closed, and then the culture box body 9 is placed on the placement seat 10 in the incubator 1.

[0027] Then, the box cover 7 is closed, and the ultraviolet disinfection lamp 8 in the mounting groove 4 on the box cover 7 is turned on to disinfect the incubator 1 so that the cells can be cultured in a sterile environment.

[0028] Then, the telescopic gas rod 35 is started through the fixing mechanism 3, and the output end of the telescopic gas rod 35 drives the vertical rod 36 to move. The movement of the vertical rod 36 drives the triangular plate 37 to rotate around the positioning rod 32, and the rotation of the triangular plate 37 drives the first cross bar 38 to move on the top of the vertical plate 34.

[0029] The movement of the first cross bar 38 drives the movement of the first fixing plate 39 , and the anti-slip pad 301 on the first fixing plate 39 moves to a position that fits the outer surface of the culture box body 9 , and is used in conjunction with the anti-slip pad 301 connected to the second fixing plate 31 to firmly fix the culture box body 9 .

[0030] Next, the motor 201 is started through the vibration mechanism 2, and the output end of the motor 201 drives one of the long rods 24 to rotate. The rotation of one of the long rods 24 cooperates with the sprocket 23 and the chain 22 to make the two circular plates 28 rotate synchronously.

[0031] The two circular plates 28 rotate synchronously with the two connecting rods 27 and the two connecting seats 202 so that the two moving rods 26 drive the two top plates 203 to move up and down continuously to vibrate the culture box body 9 on the placement seat 10.

[0032] At the same time, the guide plate 25 guides the two moving rods 26 when they move, so that the two top plates 203 move more smoothly, thereby providing a dynamic culture environment for cells and co-culturing the cells.

[0033] During the culture process, the cell culture conditions in the culture box body 9 can be observed through the transparent glass 5 in the through slot 6 .

[0034] Obviously, those skilled in the art may make various modifications and variations to the present invention without departing from the spirit and scope of the present invention. Thus, if such modifications and variations fall within the scope of the claims of the present invention and their equivalents, the present invention is intended to include such modifications and variations.

Claims

1. A cell co-culture device, comprising an incubator (1), characterized in that: The lower inner wall of the incubator (1) is fixedly connected to a placement seat (10), the inner wall of the placement seat (10) is movably connected to a culture box body (9), the inner wall of the incubator (1) is provided with a fixing mechanism (3) used in conjunction with the culture box body (9), and the lower inner wall of the incubator (1) is provided with a vibration mechanism (2) used in conjunction with the placement seat (10); The vibration mechanism (2) includes a support plate (21), the bottom end of the support plate (21) is fixedly connected to the lower inner wall of the incubator (1), and one side inner wall of the support plate (21) is rotatably connected to two long rods (24). The lower part of one end of the support plate (21) is fixedly connected to a support plate (29), and the top end of the support plate (29) is fixedly connected to a motor (201). The output end of the motor (201) passes through one end of the support plate (21) and is fixedly connected to one end of one of the long rods (24). The outer surfaces of the two long rods (24) are fixedly sleeved with sprockets (2 3), and the outer surfaces of the two sprockets (23) are meshed with a chain (22), one end of each of the two long rods (24) is fixedly connected to a circular plate (28), and one end of each of the two circular plates (28) is hinged to a connecting rod (27) through a rotating shaft, one end of each of the two connecting rods (27) is hinged to a connecting seat (202) through a rotating shaft, and the top ends of the two connecting seats (202) are fixedly connected to a moving rod (26), and the top ends of the two moving rods (26) pass through the upper inner wall of the bracket plate (21) and are fixedly connected to a top plate (203) used in conjunction with the placement seat (10).

2. The cell co-culture device according to claim 1, wherein: The fixing mechanism (3) comprises a vertical plate (34), the bottom end of the vertical plate (34) is fixedly connected to the lower inner wall of the incubator (1), one end of the vertical plate (34) is fixedly connected to a mounting seat (33), the lower inner wall of the mounting seat (33) is fixedly connected to a telescopic gas rod (35), and the output end of the telescopic gas rod (35) is fixedly connected to a vertical rod (36), the upper part of one end of the vertical rod (36) is hinged to a triangular plate (37) through a rotating shaft, and one end of the vertical plate (34) is fixedly connected to the vertical rod (36). A positioning rod (32) is connected, and a triangular plate (37) is rotatably sleeved on the outer surface of the positioning rod (32); an upper portion of one end of the triangular plate (37) is hinged to a first cross bar (38) through a rotating shaft; one end of the first cross bar (38) is fixedly connected to a first fixed plate (39); a second cross bar (302) is fixedly connected to an inner wall of one side of the incubator (1), and one end of the second cross bar (302) is fixedly connected to a second fixed plate (31) used in conjunction with the first fixed plate (39).

3. The cell co-culture device according to claim 1, wherein: A box cover (7) is hingedly connected to one side of the top of the incubator (1), and a mounting groove (4) is provided on the lower inner wall of the box cover (7). An ultraviolet disinfection lamp (8) is fixedly connected to the inner wall of the mounting groove (4).

4. The cell co-culture device according to claim 1, wherein: A through slot (6) is provided at one end of the incubator (1), and a transparent glass body (5) is fixedly connected to the inner wall of the through slot (6).

5. The cell co-culture device according to claim 1, wherein: The top ends of the two top plates (203) are flush with each other.

6. The cell co-culture device according to claim 1, wherein: A guide plate (25) used in conjunction with two moving rods (26) is fixedly connected to an inner wall of one side of the bracket plate (21), and the top ends of the two moving rods (26) both pass through the bottom end of the guide plate (25).

7. The cell co-culture device according to claim 2, wherein: The inner wall of the first crossbar (38) is slidably connected to the top of the vertical plate (34).

8. The cell co-culture device according to claim 2, wherein: The inner walls of the first fixing plate (39) and the second fixing plate (31) are both fixedly connected with anti-slip pads (301), and the inner walls of the two anti-slip pads (301) are both arc-shaped, and the two anti-slip pads (301) are distributed in a mirror image.

Images