Mouse carbon monoxide hypoxia experiment device

By introducing a peristaltic pump and activated carbon filter box into the mouse carbon monoxide hypoxia experimental device, controlling the acid reaction volume and treating carbon monoxide, the problems of device safety and poisoning risks are solved to ensure the safety of the experiment.

CN223248366UActive Publication Date: 2025-08-22FUDAN UNIVERSITY
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Patent Information

Application Number
CN202422174805.X
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-09-05
Publication Date
2025-08-22
Estimated Expiration
2034-09-05

AI Technical Summary

Technical Problem

The existing carbon monoxide hypoxia experimental device in mice lacks a safety barrier, which can easily lead to injury to the experimental personnel, and the carbon monoxide treatment is incomplete, which poses a risk of poisoning.

Method used

An experimental device including a peristaltic pump, a flask, a filter box and activated carbon was designed. The amount of acid added is controlled by the peristaltic pump to generate carbon monoxide, and the waste gas is treated with the activated carbon adsorption filter box, and the observation bottle is used to monitor gas residues.

Benefits of technology

It has achieved safe control of the amount of carbon monoxide generation, prevent splash damage, effectively treat carbon monoxide, reduce the risk of poisoning, and ensure the safety of the experiment.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model provides a mouse carbon monoxide hypoxia experiment device, and mainly relates to the technical field of biological experiment equipment. A mouse carbon monoxide hypoxia experiment device comprises a base, a box body fixedly arranged at the top of the base, a first peristaltic pump and a second peristaltic pump fixedly arranged on the inner side wall of the box body, a flask fixedly arranged on the other side wall in the box body, a formic acid bottle and a concentrated sulfuric acid bottle arranged at the bottom of the box body, and a filter box fixedly arranged on the outer side of the box body. An observation bottle is arranged on the top of the base close to the box body, and a baffle is hinged to the top of the box body. The device disclosed by the utility model has the beneficial effects that formic acid and concentrated sulfuric acid are added into the flask at a certain speed through the peristaltic pump, and the amount of acid participating in reaction can be controlled, so that the amount of generated carbon monoxide is controlled; residual carbon monoxide in an oxygen deficit experiment in the observation bottle can be adsorbed through activated carbon, whether carbon monoxide gas is left after adsorption is detected by observing whether bubbles exist after the carbon monoxide gas is discharged into water again, and leakage of the carbon monoxide can be effectively monitored and prevented.
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Description

Technical Field

[0001] The utility model mainly relates to the technical field of biological experimental equipment, in particular to a carbon monoxide hypoxia experimental device for mice. Background Art

[0002] Currently, the equipment used in carbon monoxide anoxic experiments is relatively simple and lacks safety barriers. If concentrated sulfuric acid splashes, it will cause serious harm to experimenters and students. The equipment used in most laboratories is not sophisticated enough. Improper operation may cause the concentrated sulfuric acid and formic acid to continue to react, continuously producing carbon monoxide beyond the experimental requirements.

[0003] Currently, most devices lack carbon monoxide treatment equipment. Carbon monoxide can cause poisoning, so it is necessary to treat the waste gas generated by the experimental device. Utility Model Content

[0004] In order to achieve the above-mentioned purpose, the present invention is realized through the following technical solutions:

[0005] A carbon monoxide hypoxia experimental device for mice comprises a base, a box body fixedly arranged on the top of the base, a first peristaltic pump and a second peristaltic pump fixedly arranged on the inner inner wall of the box body, a flask fixedly arranged on the other inner side wall of the box body, a formic acid bottle and a concentrated sulfuric acid bottle arranged at the bottom of the box body, a filter box fixedly arranged on the outside of the box body, an observation bottle arranged near the box body on the top of the base body, and a baffle hinged on the top of the box body.

[0006] The inlet end of the first peristaltic pump is connected to the formic acid bottle through a connecting tube, and the outlet end is connected to the flask through a connecting tube. The inlet end of the second peristaltic pump is connected to the concentrated sulfuric acid bottle through a connecting tube, and the outlet end is connected to the flask through a connecting tube. The flask is connected to the observation bottle through a connecting tube, and the observation bottle is connected to the filter box through a connecting tube.

[0007] A support rod is fixedly provided on the inner wall of the box body, a clamping claw is fixedly provided on the other end of the support rod, the inner wall of the clamping claw abuts against the outer ring of the flask, and the open end of the clamping claw is fixed by a bolt.

[0008] A support plate is fixedly arranged in an array on the inner wall of the box body, and the first peristaltic pump and the second peristaltic pump are fixedly arranged on the top of the support plate. The first peristaltic pump and the second peristaltic pump are fixed by this structure.

[0009] Activated carbon is arranged inside the filter box.

[0010] The tops of the formic acid bottle, concentrated sulfuric acid bottle and observation bottle are all abutted with sealing plugs, and each connecting pipe passes through the corresponding sealing plug. The outer ring of the observation bottle is provided with an inlet, and the end of the inlet is threadedly connected to the bottle cap.

[0011] Two cylindrical protrusions are fixedly provided near the middle position of the bottom of the box body, and the outer rings of the formic acid bottle and the concentrated sulfuric acid bottle abut against the inner walls of the corresponding cylindrical protrusions.

[0012] The box body and baffle are both made of transparent acrylic material.

[0013] Compared with the prior art, the beneficial effects of the present invention are:

[0014] The utility model has a simple structure and is easy to install and use. Formic acid and concentrated sulfuric acid are added into a flask at a certain speed through a peristaltic pump, so that the amount of acid participating in the reaction can be controlled, thereby controlling the amount of carbon monoxide produced, and facilitating the cleaning of waste liquid. The remaining carbon monoxide in the observation bottle during the oxygen deficiency experiment can be adsorbed by activated carbon, and whether there is residual carbon monoxide gas after adsorption can be checked by discharging it into water again to observe whether there are bubbles, thereby effectively monitoring and preventing carbon monoxide leakage. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Attachment Figure 1 This is a schematic diagram of the first internal structure of the utility model;

[0016] Attachment Figure 2 This is a schematic diagram of the second internal structure of the utility model;

[0017] Attachment Figure 3 It is a schematic diagram of the local structure of the utility model;

[0018] Attachment Figure 4 It is a structural schematic diagram of the filter box of the utility model.

[0019] The numbers shown in the accompanying drawings are: 1. base; 2. box body; 3. first peristaltic pump; 4. second peristaltic pump; 5. flask; 6. formic acid bottle; 7. concentrated sulfuric acid bottle; 8. filter box; 9. observation bottle; 10. baffle; 11. support rod; 12. clamping jaw; 13. support plate; 14. sealing plug; 15. cylindrical protrusion; 16. bottle cap; 17. partition. DETAILED DESCRIPTION

[0020] The present invention will be further described with reference to the accompanying drawings and specific embodiments. It should be understood that these embodiments are intended only to illustrate the present invention and are not intended to limit the scope of the present invention. Furthermore, it should be understood that after reading the contents of this invention, those skilled in the art may make various changes or modifications to the present invention, and that such equivalent forms also fall within the scope defined in this application.

[0021] In conjunction with the accompanying drawings, a mouse carbon monoxide hypoxia experimental device includes a base 1, a box 2 is fixedly arranged on the top of the base 1, a first peristaltic pump 3 and a second peristaltic pump 4 are fixedly arranged on the inner wall of the box 2, a flask 5 is fixedly arranged on the other side wall of the box 2, a formic acid bottle 6 and a concentrated sulfuric acid bottle 7 are arranged at the bottom of the box 2, a filter box 8 is fixedly arranged on the outside of the box 2, an observation bottle 9 is arranged on the top of the base 1 near the box 2, and a baffle 10 is hinged on the top of the box 2. This structural design prevents injuries to experimenters in the event of accidents such as splashing.

[0022] The inlet end of the first peristaltic pump 3 is connected to the formic acid bottle 6 through a connecting tube, and the outlet end is connected to the flask 5 through a connecting tube. The inlet end of the second peristaltic pump 4 is connected to the concentrated sulfuric acid bottle 7 through a connecting tube, and the outlet end is connected to the flask 5 through a connecting tube. The flask 5 is connected to the observation bottle 9 through a connecting tube, and the observation bottle 9 is connected to the filter box 8 through a connecting tube. This structural design controls the amount of acid participating in the reaction, thereby controlling the amount of carbon monoxide produced.

[0023] A support rod 11 is fixedly provided on the inner wall of the box body 2, and a clamping jaw 12 is fixedly provided on the other end of the support rod 11. The inner wall of the clamping jaw 12 abuts against the outer ring of the flask 5. The open end of the clamping jaw 12 is fixed by a bolt, and the flask 5 is clamped and fixed by tightening the bolt.

[0024] A support plate 13 is fixedly arranged in an array on the inner wall of the box body 2, and the first peristaltic pump 3 and the second peristaltic pump 4 are fixedly arranged on the top of the support plate 13. The first peristaltic pump 3 and the second peristaltic pump 4 are fixed through this structural design.

[0025] The interior of the filter box 8 is divided into two parts, an upper part and an lower part, which are separated by a partition 17. Several exhaust pipes are fixedly set at the bottom of the partition 17. The upper part of the filter box 8 is provided with activated carbon and the lower part is provided with water. Through this structural design, carbon monoxide is adsorbed, and carbon monoxide leakage and poisoning are monitored and prevented. A one-way valve is fixedly set on the side of the filter box 8 near the bottom of the partition 17.

[0026] The formic acid bottle 6 , the concentrated sulfuric acid bottle 7 , and the observation bottle 9 are all abutted against the tops with sealing plugs 14 , and each connecting pipe passes through the corresponding sealing plug 14 . The formic acid bottle 6 is filled with formic acid, and the concentrated sulfuric acid bottle 7 is filled with concentrated sulfuric acid.

[0027] The outer ring of the observation bottle 9 is provided with an inlet, the end of which is threadedly connected to the bottle cap 16 , and the experimental mouse enters through the inlet and is sealed by the bottle cap 16 .

[0028] Two cylindrical protrusions 15 are fixedly provided near the middle position at the bottom of the box body 2. The outer rings of the formic acid bottle 6 and the concentrated sulfuric acid bottle 7 abut against the inner walls of the corresponding cylindrical protrusions 15. This structural design prevents the formic acid bottle 6 and the concentrated sulfuric acid bottle 7 from tipping over.

[0029] The box body 2 and the baffle 10 are both made of transparent acrylic material, and the observation bottle 9 and the filter box 8 are also made of transparent material.

[0030] When the device is in use, the experimental mouse enters the observation bottle 9 through the entrance, the bottle cap 16 is screwed on, and it is ensured that all devices are connected. The baffle 10 is closed, and the formic acid in the formic acid bottle 6 and the concentrated sulfuric acid in the concentrated sulfuric acid bottle 7 are pumped into the flask 5 at a uniform speed through the first peristaltic pump 3 and the second peristaltic pump 4. The formic acid can be hydrolyzed into carbon monoxide and water under the catalysis of the concentrated sulfuric acid, and the formed carbon monoxide enters the observation bottle 9 through the connecting tube; the poisoning reaction of the mouse is observed, and when the symptoms of hypoxia are obvious, the peristaltic pump is turned off. The remaining carbon monoxide in the observation bottle 9 enters the filter box 8 and is adsorbed by the activated carbon. The presence of bubbles in the water is observed to determine whether all the carbon monoxide is adsorbed.

[0031] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A carbon monoxide hypoxia experimental device for mice, comprising a base (1), characterized in that: A box body (2) is fixedly arranged on the top of the base (1), a first peristaltic pump (3) and a second peristaltic pump (4) are fixedly arranged on the inner side wall of the box body (2), a flask (5) is fixedly arranged on the other side wall of the box body (2), a formic acid bottle (6) and a concentrated sulfuric acid bottle (7) are arranged at the bottom of the box body (2), a filter box (8) is fixedly arranged on the outside of the box body (2), an observation bottle (9) is arranged at a position close to the box body (2) on the top of the base (1), and a baffle (10) is hinged on the top of the box body (2).

2. The carbon monoxide hypoxia experimental device for mice according to claim 1, characterized in that: The inlet end of the first peristaltic pump (3) is connected to the formic acid bottle (6) through a connecting tube, and the outlet end is connected to the flask (5) through a connecting tube. The inlet end of the second peristaltic pump (4) is connected to the concentrated sulfuric acid bottle (7) through a connecting tube, and the outlet end is connected to the flask (5) through a connecting tube. The flask (5) is connected to the observation bottle (9) through a connecting tube. The tops of the formic acid bottle (6), the concentrated sulfuric acid bottle (7), and the observation bottle (9) are all abutted with sealing plugs (14). Each connecting tube passes through the corresponding sealing plug (14). The observation bottle (9) is connected to the filter box (8) through the connecting tube.

3. The carbon monoxide hypoxia experimental device for mice according to claim 1, characterized in that: A support rod (11) is fixedly provided on the inner wall of the box body (2), a clamping claw (12) is fixedly provided on the other end of the support rod (11), the inner wall of the clamping claw (12) abuts against the outer ring of the flask (5), and the open end of the clamping claw (12) is fixed by a bolt.

4. The carbon monoxide hypoxia experimental device for mice according to claim 1, characterized in that: A support plate (13) is fixedly arranged in an array on the inner wall of the box body (2), and the first peristaltic pump (3) and the second peristaltic pump (4) are fixedly arranged on the top of the support plate (13). The first peristaltic pump (3) and the second peristaltic pump (4) are fixed by this structure.

5. The carbon monoxide hypoxia experimental device for mice according to claim 1, characterized in that: Activated carbon is arranged inside the filter box (8).

6. The carbon monoxide hypoxia experimental device for mice according to claim 1, characterized in that: The outer ring of the observation bottle (9) is provided with an inlet, and the end of the inlet is threadedly connected to the bottle cap (16).

7. The carbon monoxide hypoxia experimental device for mice according to claim 1, characterized in that: Two cylindrical protrusions (15) are fixedly provided near the middle position of the bottom of the box body (2), and the outer rings of the formic acid bottle (6) and the concentrated sulfuric acid bottle (7) abut against the inner walls of the corresponding cylindrical protrusions (15).

8. The carbon monoxide hypoxia experimental device for mice according to claim 1, characterized in that: The box body (2) and the baffle (10) are both made of transparent acrylic material.