Mixing device for preparing Mito-HIBA

Abstract

The utility model relates to the field of mixing devices, in particular to a mixing device for preparing Mito-HIBA, which comprises a base and a mixing tank, an adding pipe is fixedly communicated with the top of the mixing tank, a first stop valve is fixed on a pipe body of the adding pipe, a discharging pipe is fixedly communicated with the bottom of the mixing tank, and a second stop valve is fixed on a pipe body of the discharging pipe. Supporting plates are symmetrically and vertically fixed to the two ends of the top of the base, the mixing tank is connected between the two supporting plates through a shaking assembly, a stirring rod stirring assembly is fixed in the mixing tank, a scraping assembly is fixed to the stirring rod stirring assembly, and a pneumatic stirring assembly is further fixed in the mixing tank. The mixing tank has the beneficial effects that the Mito-HIBA reagent in the mixing tank can be stirred in multiple stirring modes, the mixing efficiency of the Mito-HIBA reagent is greatly improved, the mixing effect of the Mito-HIBA reagent is favorably improved, the Mito-HIBA reagent can be effectively prevented from being adhered to the inner surface of the mixing tank, the discharging of the Mito-HIBA reagent is more thorough, the practicability is better, and the mixing tank is suitable for popularization.

Description

Technical Field

[0001] The utility model relates to the field of mixing devices, in particular to a mixing device for preparing Mito-HOBA. Background Art

[0002] Mito-HOBA is an important reagent in the biomedical field, especially in the study of mitochondrial function and related diseases. Specifically, Mito-HOBA is a mitochondrial-specific fluorescent probe that is mainly used to detect and study the generation of reactive oxygen species (ROS) in mitochondria within cells.

[0003] Although the mixing device currently used to prepare Mito-HOBA can achieve the mixing function of Mito-HOBA reagent, it usually achieves the mixing purpose by stirring with a simple stirring rod. The single mixing method leads to low mixing efficiency and affects the mixing effect, and further improvement is needed.

[0004] Therefore, it is necessary to invent a mixing device for preparing Mito-HOBA. Utility Model Content

[0005] To this end, the present invention provides a mixing device for preparing Mito-HOBA to solve the problems raised in the above background technology.

[0006] In order to achieve the above-mentioned purpose, the utility model provides the following technical solution: a mixing device for preparing Mito-HOBA, comprising a base and a mixing tank, the top of the mixing tank is fixedly connected to an addition pipe, the body of the addition pipe is fixed with a first stop valve, the bottom of the mixing tank is fixedly connected to a discharge pipe, the body of the discharge pipe is fixed with a second stop valve, support plates are symmetrically and vertically fixed at both ends of the top of the base, the mixing tank is connected between the two support plates through a shaking assembly, a stirring rod stirring assembly is fixed in the mixing tank, a scraping assembly is fixed on the stirring rod stirring assembly, and a pneumatic stirring assembly is also fixed in the mixing tank.

[0007] Preferably, the shaking assembly includes a reducer, which is fixed on one side of the top of the support plate, and a servo motor is fixed on the other side of the top of the support plate. The output shaft of the servo motor passes through the support plate, and the output shaft of the servo motor is fixedly connected to the input shaft of the reducer. The output shaft of the reducer is fixed with a first connecting plate, and the first connecting plate is fixed at the middle of the side end of the mixing tank.

[0008] Preferably, a second connecting plate is fixed to the middle of the other side end of the mixing tank, and the second connecting plate is rotatably connected to the top end of the adjacent support plate through a rotating seat.

[0009] Preferably, the stirring rod stirring assembly includes a stirring motor, which is fixed on the top of the mixing tank. The stirring motor output shaft vertically passes through the center of the top of the mixing tank, and a rotating shaft is fixed to the bottom end of the stirring motor output shaft. Several short stirring rods are fixed on the surface of the rotating shaft, and several long stirring rods are fixed on the lower side of the rotating shaft surface.

[0010] Preferably, the scraping assembly includes an L-shaped connecting rod, the surface of the L-shaped connecting rod is fixedly connected to the ends of several long stirring rods, a rubber scraper is fixed to the surface of the L-shaped connecting rod, and the surface of the rubber scraper abuts against the side of the inner wall of the mixing tank.

[0011] Preferably, the pneumatic stirring assembly includes a fan, the bottom of the fan is fixed on the top of the mixing tank, the fan input end is fixedly connected to an air inlet hood, a first dust filter is fixed inside the air inlet hood, the fan output end vertically passes through the top of the mixing tank, and the fan output end is fixedly connected to an air supply pipe, the air supply pipe is located between the short stirring rod and the L-shaped connecting rod, the bottom end of the air supply pipe is fixedly connected to an annular exhaust pipe, the rotating shaft vertically passes through the annular exhaust pipe, the annular exhaust pipe is located below several short stirring rods, and several exhaust holes are opened on the top of the annular exhaust pipe.

[0012] Preferably, an air outlet is provided on the top of the mixing tank, and a second dust-proof filter is fixed inside the air outlet.

[0013] The beneficial effects of the utility model are as follows: through the coordinated use of the provided base, mixing tank, addition pipe, first stop valve, discharge pipe, second stop valve, support plate, shaking assembly, stirring rod stirring assembly, scraping assembly and pneumatic stirring assembly, the Mito-HOBA reagent in the mixing tank can be stirred by multiple stirring methods such as shaking stirring, stirring rod stirring and pneumatic stirring, thereby greatly improving the mixing efficiency of the Mito-HOBA reagent, and facilitating improving the mixing effect of the Mito-HOBA reagent. It can also effectively prevent the Mito-HOBA reagent from adhering to the inner surface of the mixing tank, which is conducive to more thorough discharge of the Mito-HOBA reagent. The utility model has good practicality and is suitable for promotion. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 A cross-sectional view of the structure provided by the utility model;

[0015] Figure 2 for Figure 1 A magnified view of the structure at point A;

[0016] Figure 3 for Figure 1 A magnified view of the structure at point B in FIG;

[0017] Figure 4 This is a cross-sectional view of the structure of the annular air outlet duct provided by the utility model;

[0018] Figure 5 This is the main view of the structure provided by the utility model.

[0019] In the figure: 1. Base; 2. Mixing tank; 3. Adding pipe; 4. First stop valve; 5. Discharge pipe; 6. Second stop valve; 7. Support plate; 8. Reducer; 9. Servo motor; 10. First connecting plate; 11. Second connecting plate; 12. Rotating seat; 13. Stirring motor; 14. Rotating shaft; 15. Short stirring rod; 16. Stirring rod; 17. L-shaped connecting rod; 18. Rubber scraper; 19. Fan; 20. Air inlet hood; 21. First dust filter; 22. Air duct; 23. Annular exhaust duct; 24. Exhaust hole; 25. Air outlet; 26. Second dust filter. DETAILED DESCRIPTION

[0020] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention and are not used to limit the present invention.

[0021] Please refer to the attached Figure 1-Figure 5 The utility model provides a mixing device for preparing Mito-HOBA, comprising a base 1 and a mixing tank 2, wherein the top of the mixing tank 2 is fixedly connected with an addition pipe 3, and the body of the addition pipe 3 is fixed with a first stop valve 4 for adding Mito-HOBA into the mixing tank 2 to prepare the required reagent, the bottom of the mixing tank 2 is fixedly connected with a discharge pipe 5, and the body of the discharge pipe 5 is fixed with a second stop valve 6 for discharging the mixed Mito-HOBA reagent, support plates 7 are symmetrically and vertically fixed at both ends of the top of the base 1, the mixing tank 2 is connected between the two support plates 7 by a shaking assembly, a stirring rod stirring assembly is fixed in the mixing tank 2, a scraping assembly is fixed on the stirring rod stirring assembly, and a pneumatic stirring assembly is also fixed in the mixing tank 2;

[0022] The shaking assembly includes a reducer 8. It should be noted that the reducer is a transmission device for low speed and high torque. The power of the electric motor, internal combustion engine or other high-speed operation is achieved by meshing the gear with a small number of teeth on the input shaft of the reducer with the large gear on the output shaft. The reducer 8 is fixed to one side of the top of the support plate 7, and a servo motor 9 is fixed to the other side of the top of the support plate 7. The output shaft of the servo motor 9 passes through the support plate 7, and the output shaft of the servo motor 9 is fixedly connected to the input shaft of the reducer 8. The output shaft of the reducer 8 is fixed with a first connecting plate 10, which is fixed to the middle of the side end of the mixing tank 2, and a second connecting plate 11 is fixed to the middle of the other side end of the mixing tank 2. The second connecting plate 11 is rotatably connected to the top of the adjacent support plate 7 through a rotating seat 12. Specifically, under the deceleration action of the reducer 8, when the servo motor 9 is running, the mixing tank 2 can be driven to rotate forward and backward between the two support plates 7, thereby realizing the function of shaking the mixing tank 2, so that the Mito-HOBA reagent in the mixing tank 2 can be shaken and stirred;

[0023] The stirring rod stirring assembly includes a stirring motor 13. It should be noted that the stirring motor 13 is preferably set to a variable frequency motor. The stirring motor 13 is fixed to the top of the mixing tank 2. The output shaft of the stirring motor 13 vertically passes through the center of the top of the mixing tank 2, and a rotating shaft 14 is fixed to the bottom end of the output shaft of the stirring motor 13. A plurality of short stirring rods 15 are fixed to the surface of the rotating shaft 14, and a plurality of long stirring rods 16 are fixed to the lower side of the surface of the rotating shaft 14. Specifically, when the stirring motor 13 is running, the plurality of short stirring rods 15 and the long stirring rods 16 can be driven by the rotating shaft 14 to rotate in the mixing tank 2, thereby stirring the Mito-HOBA reagent in the mixing tank 2;

[0024] The scraping assembly includes an L-shaped connecting rod 17, the surface of the L-shaped connecting rod 17 is fixedly connected to the ends of several long stirring rods 16, and a rubber scraper 18 is fixed to the surface of the L-shaped connecting rod 17. The surface of the rubber scraper 18 abuts against the side of the inner wall of the mixing tank 2. Specifically, when the stirring motor 13 drives the long stirring rod 16 to rotate through the rotating shaft 14, it can drive the L-shaped connecting plate 17 to rotate synchronously, so that the rubber scraper 18 can scrape the inner surface of the mixing tank 2, thereby effectively preventing the Mito-HOBA reagent from adhering to the inner surface of the mixing tank 2, which is conducive to more thorough discharge of the Mito-HOBA reagent.

[0025] The pneumatic stirring assembly includes a fan 19, the bottom of the fan 19 is fixed to the top of the mixing tank 2, the input end of the fan 19 is fixedly connected to an air inlet cover 20, a first dust filter 21 is fixed inside the air inlet cover 20, the output end of the fan 19 vertically passes through the top of the mixing tank 2, and the output end of the fan 19 is fixedly connected to an air duct 22, the air duct 22 is located between the short stirring rod 15 and the L-shaped connecting rod 17, the bottom end of the air duct 22 is fixedly connected to an annular exhaust pipe 23, the rotating shaft 14 vertically passes through the annular exhaust pipe 23, that is, the arrangement of the air duct 22 and the annular exhaust pipe 23 will not affect the rotating shaft 14, the several short stirring rods 15 and the long stirring rod 16 During normal rotation, the annular exhaust pipe 23 is located below the plurality of short stirring rods 15, a plurality of exhaust holes 24 are provided on the top of the annular exhaust pipe 23, an air outlet 25 is provided on the top of the mixing tank 2, and a second dust filter 26 is fixed inside the air outlet 25. Specifically, when the fan 19 is running, the outside air can be sucked in through the air inlet hood 20, filtered and accelerated to form wind force, and then transported to the annular exhaust pipe 23 through the air delivery pipe 22, and discharged from the plurality of exhaust pipes 24 to form bubbles, which agitate the Mito-HOBA reagent in the mixing tank 2 to flow, thereby performing pneumatic stirring operation on the Mito-HOBA reagent in the mixing tank 2;

[0026] In summary, the device can stir the Mito-HOBA reagent in the mixing tank 2 by multiple stirring methods such as shaking stirring, stirring rod stirring and pneumatic stirring, which greatly improves the mixing efficiency of the Mito-HOBA reagent and is beneficial to improving the mixing effect of the Mito-HOBA reagent.

[0027] It should be noted that the device is electrically connected to an external main controller and 220V mains electricity, and the main controller can be a computer or other existing technical equipment for control.

[0028] The above description is merely a preferred embodiment of the present invention. Anyone skilled in the art can utilize the above-described technical solutions to modify the present invention or create equivalent technical solutions. Therefore, any simple modification or equivalent replacement based on the technical solutions of the present invention falls within the scope of protection claimed by the present invention.

Claims

1. A mixing device for preparing Mito-HOBA, comprising a base (1) and a mixing tank (2), wherein the top of the mixing tank (2) is fixedly connected to an addition pipe (3), the body of the addition pipe (3) is fixedly connected to a first stop valve (4), the bottom of the mixing tank (2) is fixedly connected to a discharge pipe (5), the body of the discharge pipe (5) is fixedly connected to a second stop valve (6), and support plates (7) are symmetrically and vertically fixed at both ends of the top of the base (1), characterized in that: The mixing tank (2) is connected between two support plates (7) via a shaking assembly. A stirring rod stirring assembly is fixed in the mixing tank (2), a scraping assembly is fixed on the stirring rod stirring assembly, and a pneumatic stirring assembly is also fixed in the mixing tank (2).

2. A mixing device for preparing Mito-HOBA according to claim 1, characterized in that: The shaking assembly includes a reducer (8), the reducer (8) is fixed to one side of the top of the support plate (7), a servo motor (9) is fixed to the other side of the top of the support plate (7), the output shaft of the servo motor (9) passes through the support plate (7), and the output shaft of the servo motor (9) is fixedly connected to the input shaft of the reducer (8), the output shaft of the reducer (8) is fixed with a first connecting plate (10), and the first connecting plate (10) is fixed to the middle part of the side end of the mixing tank (2).

3. A mixing device for preparing Mito-HOBA according to claim 2, characterized in that: A second connecting plate (11) is fixed to the middle of the other side end of the mixing tank (2), and the second connecting plate (11) is rotatably connected to the top end of the adjacent support plate (7) through a rotating seat (12).

4. A mixing device for preparing Mito-HOBA according to claim 1, characterized in that: The stirring rod stirring assembly comprises a stirring motor (13), the stirring motor (13) is fixed on the top of the mixing tank (2), the output shaft of the stirring motor (13) vertically passes through the center of the top of the mixing tank (2), and a rotating shaft (14) is fixed at the bottom end of the output shaft of the stirring motor (13), a plurality of short stirring rods (15) are fixed on the surface of the rotating shaft (14), and a plurality of long stirring rods (16) are fixed on the lower side of the surface of the rotating shaft (14).

5. A mixing device for preparing Mito-HOBA according to claim 4, characterized in that: The scraping assembly comprises an L-shaped connecting rod (17), the surface of which is fixedly connected to the ends of a plurality of long stirring rods (16), a rubber scraper (18) being fixed to the surface of the L-shaped connecting rod (17), and the surface of the rubber scraper (18) being in contact with the inner wall side of the mixing tank (2).

6. A mixing device for preparing Mito-HOBA according to claim 5, characterized in that: The pneumatic stirring assembly includes a fan (19), the bottom of the fan (19) is fixed on the top of the mixing tank (2), the input end of the fan (19) is fixedly connected to an air inlet cover (20), a first dust filter (21) is fixed inside the air inlet cover (20), the output end of the fan (19) vertically passes through the top of the mixing tank (2), and the output end of the fan (19) is fixedly connected to an air supply pipe (22), the air supply pipe (22) is located between the short stirring rod (15) and the L-shaped connecting rod (17), the bottom end of the air supply pipe (22) is fixedly connected to an annular exhaust pipe (23), the rotating shaft (14) vertically passes through the annular exhaust pipe (23), the annular exhaust pipe (23) is located below a plurality of short stirring rods (15), and a plurality of exhaust holes (24) are opened on the top of the annular exhaust pipe (23).

7. A mixing device for preparing Mito-HOBA according to claim 6, characterized in that: An air outlet (25) is provided on the top of the mixing tank (2), and a second dustproof filter (26) is fixed inside the air outlet (25).

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