Sealing structure for puncture-free sampling and solvent volatilization prevention

Abstract

The utility model discloses a sealing structure for puncture-free sampling and solvent volatilization prevention, and belongs to the technical field of experimental consumables. The puncture-free sampling sealing structure capable of preventing the solvent from volatilizing comprises a sealing cover and a sample tube, wherein the sealing cover consists of a cover edge part, an anti-convection surrounding wall, a reverse arc-shaped surrounding wall and a capillary hole in sequence. According to the puncture-free sampling sealing structure capable of preventing the solvent from volatilizing, after the sealing cover is mounted at the top of a sample tube, the air flow rate of a liquid evaporation surface can be reduced to a certain extent, and a volatile reagent is condensed on the outer wall of the reverse arc-shaped surrounding wall in the process of volatilizing and moving upwards; the condensed liquid beads flow to the positions of capillary holes in the bottom of the sealing cover along the outer wall, so that the condensed liquid beads continuously flow back into the sample tube, and part of the volatile reagent is cooled due to the capillary action, and the condensed liquid beads can stay in the capillary holes due to siphoning to form a siphon liquid column; the solvent evaporation speed is further reduced; and meanwhile, the scheme has the effects of simple structure and relatively low cost.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental consumables, in particular to a sealing structure for puncture-free sampling to prevent solvent volatilization. Background Art

[0002] In daily work, many scenarios require the handling of solvents with low boiling points, easy volatility, or toxic and harmful substances. For example, in liquid chromatography-mass spectrometry, methanol or acetonitrile is needed to extract and precipitate proteins in samples during chromatographic pretreatment to form solvent substances. The volatilization of these solvent substances can cause laboratory contamination, changes in sample concentration, and experimental failure.

[0003] Currently, the traditional method to prevent solvent volatilization is to use special silicone pads, sealing films, sealing covers and other sealing methods. When sampling is required, a lower temperature environment such as opening the lid of an ice box is usually adopted. Another method is to add paraffin oil to the sample surface for sealing before sampling. Another scenario is that the sampling needle directly punctures the silicone pad, sealing film, sealing cover, etc. for closed sampling to reduce the loss of easily evaporated solvents.

[0004] However, the existing operation on the ice box to ensure the constant temperature of the environment is a cumbersome and costly method, and the cost of paraffin oil sealing is high, and sample volatilization cannot be avoided during the addition of paraffin oil. In addition, the injection needle directly and repeatedly punctures the sealing structures such as the silicone pad, sealing film, and sealing cover, which will cause the silicone pad, sealing film, sealing cover and other sealing structures to be damaged during the sample aspiration and injection process, resulting in debris enrichment, thereby causing blockage and failure of the pipelines of sampling and analysis instruments such as liquid chromatography-mass spectrometry. In addition, the long-term and high-frequency mechanical puncture action will cause mechanical wear of the liquid chromatography-mass spectrometry instrument, the chromatography pretreatment injection needle, the drive motor, etc., thereby reducing the service life of the instrument. Utility Model Content

[0005] In response to the problems existing in the above-mentioned prior art, the utility model provides a sealing structure for puncture-free sampling to prevent solvent volatilization, which realizes the sealing of the sealing cover by condensation reflux, and ensures the technical effect of relatively long-term sealed storage of volatile solvents.

[0006] In order to solve the above technical problems and achieve the above technical effects, the present invention is implemented through the following technical solutions:

[0007] The technical solution of the utility model is: a sealing structure for puncture-free sampling to prevent solvent volatilization, comprising a sealing cover and a sample tube; the sealing cover comprises a cover edge, an anti-convection surrounding wall capable of preventing gas convection, an inverted arc surrounding wall and a capillary hole, the anti-convection surrounding wall is fixedly installed on the bottom of the cover edge, the inverted arc surrounding wall is arranged at the bottom of the anti-convection surrounding wall, and the capillary hole is fixedly installed on the bottom of the inverted arc surrounding wall; the top of the sample tube is provided with an opening adapted to the sealing cover, the diameter of the opening is larger than the diameter of the anti-convection surrounding wall, and the diameter of the sample tube is the same as the diameter of the sealing cover, and the interior of the sample tube is provided with a storage cavity capable of storing the solvent.

[0008] Furthermore, the sealing cover is a circular sealing cover, the cover edge is a circular cover edge, the inner wall of the anti-arc surrounding wall is the circular anti-arc inner wall of the circular sealing cover inner cavity, the outer wall of the anti-arc surrounding wall is the anti-arc outer wall of the circular sealing cover, the capillary holes are capillary holes at the bottom of the circular sealing cover, the anti-convection surrounding wall is the anti-convection annular wall of the circular sealing cover inner cavity, and the anti-convection annular wall of the circular sealing cover inner cavity is arranged in a circular ring shape.

[0009] Furthermore, the inner wall of the capillary hole at the bottom of the circular sealing cover is provided with an inner wall of the capillary hole at the bottom of the circular sealing cover fixedly connected to the circular reverse arc inner wall of the circular sealing cover inner cavity, and the anti-convection inner wall of the circular sealing cover inner cavity, the circular reverse arc inner wall of the circular sealing cover inner cavity and the inner wall of the capillary hole at the bottom of the circular sealing cover constitute the circular sealing cover inner cavity.

[0010] Furthermore, the diameter of the anti-convection inner wall of the circular sealing cover cavity is smaller than the diameter of the circular cover edge.

[0011] Furthermore, the sample tube is a circular sample tube, and the storage cavity is the inner cavity of the circular sample tube.

[0012] Furthermore, the sealing cover is a square sealing cover, the cover edge is a square sealing cover edge, the anti-convection surrounding wall is an anti-convection annular wall of the inner cavity of the square sealing cover, the inner wall of the anti-arc surrounding wall is the anti-arc surrounding wall of the square sealing cover, the anti-arc surrounding wall of the square sealing cover is square and fixedly installed on the bottom of the square sealing cover edge, and the capillary hole is a capillary hole at the bottom of the square sealing cover.

[0013] Furthermore, the square sealing cover, the anti-convection annular wall of the square sealing cover inner cavity and the inner wall of the square sealing cover surrounded by the reverse arc form the square sealing cover inner cavity connected to the capillary hole at the bottom of the square sealing cover.

[0014] Furthermore, the sample tube is a square sample tube, and the storage cavity is the inner cavity of the square sample tube.

[0015] Furthermore, it also includes a sealing cover plate, wherein multiple sealing covers are arranged at intervals along the width direction of the sealing cover plate, and the multiple sealing covers in the width direction form a sealing cover group, and multiple sealing cover groups are arranged at intervals along the length direction of the sealing cover plate, and a corresponding sample tube is provided at the bottom of each sealing cover.

[0016] Furthermore, an oblique groove is provided at one end of the rear side of the sealing cover plate, and a groove is provided on the right side of the sealing cover plate.

[0017] The beneficial technical effects of the utility model are:

[0018] (1) The utility model provides a sealing structure for preventing solvent volatilization during puncture-free sampling. By setting the reverse-arc surrounding wall and the capillary hole, when the sealing cover is installed on the top of the sample tube, only one capillary hole is left, which can reduce the air flow rate of the liquid evaporation surface to a certain extent. In the process of volatile upward movement, the volatile reagent condenses on the outer wall of the reverse-arc surrounding wall, and after forming liquid droplets on the outer wall of the reverse-arc surrounding wall, it is affected by gravity and flows along the outer wall of the reverse-arc surrounding wall to the position of the capillary hole at the bottom of the sealing cover. The condensed liquid droplets are continuously replenished into the capillary hole at the bottom of the sealing cover or flow back into the sample tube. Part of the liquid droplets will stay in the capillary hole due to capillary action, forming a siphon liquid column of the volatile reagent condensed liquid droplets when it encounters cold, forming a seal, further reducing the rate of solvent volatilization. In addition, the scheme has a simple structure and low cost, which solves the problem of high cost of traditional sealing structures.

[0019] (2) The utility model provides a sealing structure for puncture-free sampling to prevent solvent volatilization. By setting the maximum diameter of the inner wall of the reverse arc surrounding wall to be much larger than the outer aperture size of the sampling device such as a glass capillary, a sampling needle, a hard plastic tube, etc., the glass capillary, the sampling needle, the hard plastic tube, etc. can be assisted in positioning by the inner wall of the reverse arc surrounding wall when sampling, thereby reducing the calibration time, eliminating the need for long-term and high-frequency mechanical puncture action of the sampling instrument, avoiding scratches on the sealing cover, causing debris to fall into the liquid, and making the pre-treatment instrument, liquid chromatography-mass spectrometry, etc. The problem of pipeline blockage caused by analytical instruments such as coupling instruments and chromatographs can be solved, and the auxiliary positioning can further reduce the mechanical wear of the sampling instrument's injection needle, drive motor, etc., increase the service life of the sampling instrument, reduce the risk of sampling failure and reduce the effect of sampling instrument failure, and avoid the problem of manual sampling in the traditional volatile reagent sampling process. Due to the small sample tube mouth, the sampling device is prone to touch the sample tube mouth or inner wall, thereby causing reagent loss, and in the process of automated sampling, the sampling position positioning calibration needs to be performed frequently.

[0020] (3) The utility model provides a sealing structure for puncture-free sampling to prevent solvent volatilization. By setting a reverse-arc surrounding wall, the inner wall of the reverse-arc surrounding wall can be designed according to the outer aperture size of the actual pipetting tool for sampling, such as a glass capillary, a sampling needle, a hard plastic tube, etc., and the reagent remaining on the outer wall of the sampling device can be scraped when the new sealing cover is pulled out of the sampling device, thereby forming a liquid column on the inner wall of the capillary hole at the bottom of the sealing cover to continue to seal the sample tube or flow back into the sample tube, thereby saving volatile reagents and efficiently and continuously sealing the volatile reagents in the sample tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic front cross-sectional view of the circular sealing structure assembly of the present invention;

[0022] Figure 2 This is a schematic top view of the structure of the circular sealing cover and the circular sample jar of the utility model;

[0023] Figure 3 It is a structural stereoscopic diagram of a circular sealing cover and a circular sample jar of the utility model;

[0024] Figure 4 This is a front cross-sectional schematic diagram of the circular sealing cover of the present invention;

[0025] Figure 5 This utility model Figure 1 Schematic diagram of the volatilization movement direction of the sample reagent;

[0026] Figure 6 This is a frontal perspective schematic diagram of the structure of the sealing cover plate and the inner cavity of the circular sealing cover of the utility model;

[0027] Figure 7 This is a three-dimensional back view of the structure of the sealing cover plate and the inverted arc-shaped outer wall of the circular sealing cover of the utility model;

[0028] Figure 8 This is a front cross-sectional schematic diagram of the structure of the square sealing cover and the square sample tube of the utility model;

[0029] Figure 9 This utility model Figure 8 Schematic diagram of structural explosion;

[0030] Figure 10 This utility model Figure 8 Schematic diagram of the structure in ;

[0031] Figure 11 The utility model is a three-dimensional schematic structural diagram of the inverted arc surrounding wall of the square sealing cover and the capillary holes at the bottom of the square sealing cover.

[0032] Figure 12 This utility model Figure 8 Schematic diagram of the volatilization movement direction of the sample reagent;

[0033] Figure 13 This is a front view schematic diagram of the structure of the sealing cover plate and the inner cavity of the square sealing cover of the utility model;

[0034] Figure 14 It is a structural stereoscopic schematic diagram of the sealing cover plate and the square sealing cover anti-arc surrounding wall of the utility model.

[0035] The numbers and letters in the figure represent the corresponding component names:

[0036] 1. Round sealing cover; 2. Round sample tube; 3. Round cover edge; 4. Inner cavity of round sealing cover; 5. Inner cavity of round sample tube; 6. Anti-arc outer wall of round sealing cover; 7. Capillary hole at the bottom of round sealing cover; 8. Anti-arc inner wall of inner cavity of round sealing cover; 9. Inner wall of capillary hole at the bottom of round sealing cover; 10. Anti-convection inner wall of inner cavity of round sealing cover; 11. Square sealing cover; 12. Square sample tube; 13. Square sealing cover edge; 14. Inner cavity of square sealing cover; 15. Inner cavity of square sample tube; 16. Anti-arc surrounding wall of square sealing cover; 17. Capillary hole at the bottom of square sealing cover; 18. Groove; 19. Inclined groove; 20. Sealing cover plate; 21. Anti-convection annular wall of inner cavity of square sealing cover. DETAILED DESCRIPTION

[0037] In order to more clearly understand the technical means of the present invention and implement it according to the contents of the specification, the specific implementation methods of the present invention are further described in detail below in conjunction with the drawings and examples. The following examples are used to illustrate the present invention but are not used to limit the scope of the present invention.

[0038] See attached Figure 1-4 As shown, the sealing structure for puncture-free sampling to prevent solvent volatilization in embodiment 1 includes a sealing cover and a sample tube; the sealing cover includes a cover edge, an anti-convection surrounding wall capable of preventing gas convection, an anti-arc surrounding wall and capillaries capable of guiding the sampling device to easily slide in or pass through. The sealing cover can be processed into different materials according to different sample tube sealing processing requirements, such as precision injection molding into a disposable silicone cover for liquid chromatography-mass spectrometry sample loading analysis, a disposable thermoplastic polyurethane elastomer (TPE) cover for sample loading analysis of particles such as cells, and disposable polyethylene for sample loading analysis of easily corrosive chemical samples, etc.

[0039] The sealing cover is a circular sealing cover 1, the cover edge is a circular cover edge 3, the inner wall of the anti-arc surrounding wall is a circular anti-arc inner wall 8 of the circular sealing cover inner cavity, the outer wall of the anti-arc surrounding wall is a circular sealing cover anti-arc outer wall 6, the capillary hole is a circular sealing cover bottom capillary hole 7, the anti-convection surrounding wall is a circular sealing cover inner cavity anti-convection annular wall 10, and the circular sealing cover inner cavity anti-convection annular wall 10 is arranged in a circular ring shape.

[0040] The overall structure of the sealing cover in this solution is simple and easy to process. Compared with the traditional sealing cover, it does not increase the production and processing cost while adding the puncture-free sealing function.

[0041] The circular sealing cover 1 is usually made of a material with a certain degree of elasticity such as silicone by lamination, compression molding or injection molding. The size of the capillary hole 7 at the bottom of the circular sealing cover is designed according to the outer aperture size of the actual pipetting tool for sample aspiration, such as a glass capillary, a sampling needle, a hard plastic tube, etc. The diameter is usually about 1-5 mm, and the diameter can also be enlarged or reduced according to special needs.

[0042] The anti-convection surrounding wall is fixedly installed on the bottom of the cover edge, the anti-arc surrounding wall is arranged at the bottom of the anti-convection surrounding wall, and the capillary hole is fixedly installed on the bottom of the anti-arc surrounding wall; the circular sealing cover inner cavity anti-convection annular wall 10 is fixedly installed on the bottom of the circular cover edge 3, the anti-arc surrounding wall is fixedly installed on the bottom of the circular sealing cover inner cavity anti-convection annular wall 10, and the circular sealing cover bottom capillary hole 7 is fixedly installed on the bottom of the anti-arc surrounding wall, and the inner wall of the circular sealing cover bottom capillary hole 7 is provided with a circular sealing cover bottom capillary hole inner wall 9 fixedly connected to the circular sealing cover inner cavity circular anti-arc inner wall 8, the circular sealing cover inner cavity anti-convection inner wall 10, the circular sealing cover inner cavity circular anti-arc inner wall 8 and the circular sealing cover bottom capillary hole inner wall 9 constitute the circular sealing cover inner cavity 4.

[0043] By setting the reverse arc surrounding wall and the capillary hole 7 at the bottom of the circular sealing cover, when the circular sealing cover 1 is installed on the top of the circular sample tube 2, the circular sealing cover 1 seals the top of the circular sample tube 2, leaving only a hole of the capillary hole 7 at the bottom of the circular sealing cover, which can reduce the air flow rate of the liquid evaporation surface at the bottom of the circular sealing cover 1 to a certain extent, thereby reducing evaporation, and the circular reverse arc inner wall 8 of the inner cavity of the circular sealing cover can guide the sampling device to easily slide into or through the capillary hole 7 at the bottom of the circular sealing cover. During the sliding process, the sampling device will not scratch or destroy the sealing structure, thereby solving the problem that the circular sealing cover 1 is scratched, resulting in damage to the circular sealing cover 1, and debris falling into the liquid, causing pipeline blockage failure of analytical instruments such as liquid chromatography-mass spectrometry and chromatograph.

[0044] The anti-convection inner wall 10 of the circular sealing cover cavity in this solution can fully reduce the air flow rate of the liquid evaporation surface of the capillary pores 7 at the bottom of the circular sealing cover and the circular sealing cover cavity 4, thereby reducing the volatilization speed.

[0045] The top of the sample tube is provided with an opening adapted to the sealing cover. The diameter of the opening is larger than the diameter of the anti-convection surrounding wall, and the diameter of the sample tube is the same as the diameter of the sealing cover. The interior of the sample tube is provided with a storage cavity for storing the solvent. The sample tube is a circular sample tube 2, and the storage cavity is a circular sample tube inner cavity 5. The sample tube can be a circular collection tube, such as a circular test tube, a circular centrifuge tube, etc.

[0046] The diameter of the anti-convection inner wall 10 of the circular sealing cover cavity is smaller than the diameter of the circular cover edge 3 .

[0047] By designing that the diameter of the opening is larger than the diameter of the anti-convection surrounding wall, the staff can assemble the circular sealing cover 1 and the circular sample tube 2 by interference fit through physical pressing or threaded tightening, thereby sealing and preserving the sample in the inner cavity 5 of the circular sample tube.

[0048] See attached Figure 5 As shown, the two upward arrows indicate the volatilization movement direction of the sample reagent, and the two inward arrows indicate the flow direction of the volatile reagent when it is cooled. Through the arrangement of the anti-arc outer wall 6 of the circular sealing cover, the volatile reagent condenses on the anti-arc outer wall 6 of the circular sealing cover during the upward volatilization movement, and after forming liquid droplets on the anti-arc outer wall 6 of the circular sealing cover, they are affected by gravity and flow along the anti-arc outer wall 6 of the circular sealing cover to the position of the inner wall 9 of the capillary hole at the bottom of the circular sealing cover. The condensed liquid droplets are continuously replenished into the capillary hole 7 at the bottom of the circular sealing cover or flow back into the circular sample tube 2, and part of the liquid droplets will stay in the capillary hole 7 at the bottom of the circular sealing cover due to capillary action due to siphoning to form a siphon liquid column of condensed liquid droplets of the volatile reagent when it is cooled, thereby reducing the evaporation rate of the liquid on the inner wall of the capillary hole.

[0049] Through the design of the anti-convection inner wall 10 of the circular sealing cover cavity, according to Dalton's law of evaporation, the relationship between the evaporation rate of the evaporation surface and the factors affecting evaporation is W=C(Ee) / p, {W is the liquid surface evaporation rate; Ee is the saturation difference of the air, where E is the saturated air pressure at the liquid surface gas temperature, e is the actual air pressure of the air on the liquid surface; P is the air pressure; C is a proportional coefficient related to the wind speed}, the evaporation surface is proportional to the wind speed of the liquid surface, and the diameter of the circular sealing cover cavity 4 is much larger than the capillary pores 7 at the bottom of the circular sealing cover, wherein the anti-convection inner wall 10 of the circular sealing cover cavity is mainly used to greatly reduce the air flow rate on the surface of the siphon liquid column 23 of the volatile reagent when it encounters cold condensation liquid beads and the ambient air flow rate above the circular sealing cover edge 3, thereby greatly reducing the evaporation rate of the siphon liquid column of the volatile reagent when it encounters cold condensation liquid beads, thereby ensuring that the sample in the circular sample tube cavity 5 and the environment can be isolated and sealed for a long time.

[0050] Based on the above principle, this solution can also be adopted by adding a certain small amount of pure water, paraffin oil or sample solution into the inner cavity 4 of the circular sealing cover, so that a certain small amount of pure water, paraffin oil or sample solution will flow into the capillary holes 7 at the bottom of the circular sealing cover along the circular reverse arc inner wall 8 of the inner cavity of the circular sealing cover under the action of gravity, and be retained in the capillary holes 7 at the bottom of the circular sealing cover under the action of capillary siphon to form a volatile reagent condensation liquid droplets siphon liquid column when it encounters cold, thereby isolating and sealing the sample in the inner cavity 5 of the circular sample tube from the environment.

[0051] See attached Figure 6-7 As shown, multiple sealing covers are arranged at intervals along the width direction of the sealing cover plate 20, and the multiple sealing covers in the width direction form a sealing cover group, and the sealing cover group is arranged at intervals along the length direction of the sealing cover plate 20. A corresponding sample tube is provided at the bottom of each sealing cover, and the sample tube is a circular sample tube 2, and the storage cavity is a circular sample tube inner cavity 5. An inclined groove 19 is provided at one end of the rear side of the sealing cover plate 20, and a groove 18 is provided on the right side of the sealing cover plate 20. When the sealing cover plate 20 is in use, the sample tube is first installed correspondingly at the bottom of the sealing cover, and then the sealing cover plate 20 is buckled on the storage box.

[0052] By setting the inclined groove 19 and the groove 18, the inner wall of the storage box can be corresponded to facilitate the staff to disassemble the entire sealing cover 20, and the sealing cover on the sealing cover 20 can also be processed into various quantities according to needs, such as 8-hole array combination, 48-hole array combination, 96-hole array combination or 384-hole array combination, etc., to meet the needs of different specifications.

[0053] See attached Figure 8-11 As shown, the sealing structure for puncture-free sampling to prevent solvent volatilization of Example 2 includes a sealing cover and a sample tube; the sealing cover includes a cover edge, an anti-convection surrounding wall capable of preventing gas convection, an anti-arc surrounding wall and capillary pores. The sealing cover can be processed into different materials according to different sealing processing requirements of the sample tube, such as a disposable silicone cover for liquid chromatography-mass spectrometry sample loading analysis, a disposable thermoplastic polyurethane elastomer (TPU) cover for sample loading analysis of particles such as cells, and a disposable polyethylene cover for sample loading analysis of easily corrosive chemical samples, etc.

[0054] The anti-convection surrounding wall is fixedly installed on the bottom of the cover edge, the reverse arc surrounding wall is arranged on the bottom of the anti-convection surrounding wall, and the capillary hole is fixedly installed on the bottom of the reverse arc surrounding wall.

[0055] The sealing cover is a square sealing cover 11, the cover edge is a square sealing cover edge 13, the anti-convection surrounding wall is a square sealing cover inner cavity anti-convection annular wall 21, the inner wall of the anti-arc surrounding wall is a square sealing cover anti-arc surrounding wall 16, the square sealing cover anti-arc surrounding wall 16 is square and fixedly installed at the bottom of the square sealing cover edge 13, the capillary hole is a square sealing cover bottom capillary hole 17, the square sealing cover 11, the square sealing cover inner cavity anti-convection annular wall 21 and the inner wall of the square sealing cover anti-arc surrounding constitute a square sealing cover inner cavity 14 connected to the square sealing cover bottom capillary hole 17.

[0056] By setting the square sealing cover's anti-arc surrounding wall 16 and the square sealing cover's bottom capillary hole 17, when the square sealing cover 11 is installed on the top of the square sample tube 12, only a hole the size of the square sealing cover's bottom capillary hole 17 is left, which can reduce the air flow rate on the liquid evaporation surface at the bottom of the square sealing cover 11 to a certain extent, thereby reducing evaporation, and the inner wall of the square sealing cover's anti-arc surrounding wall 16 can guide the sampling device to easily slide into or through the square sealing cover's bottom capillary hole 17. During the sliding process, the sampling device will not scratch or destroy the sealing structure, thereby solving the problem of the square sealing cover 11 being scratched, causing the square sealing cover 11 to be damaged, and debris falling into the liquid, causing pipeline blockage failures in analytical instruments such as liquid chromatography-mass spectrometry and chromatographs.

[0057] The square sealing cover 11 is usually made of a material with a certain degree of elasticity such as silicone by lamination, compression molding or injection molding. The size of the capillary hole 17 at the bottom of the square sealing cover is designed according to the outer aperture size of the actual pipetting tool for sample aspiration, such as a glass capillary, a sampling needle, a hard plastic tube, etc. The diameter is usually about 1-5 mm, and the diameter can also be expanded or reduced according to special needs.

[0058] The top of the sample tube is provided with an opening adapted to the sealing cover. The diameter of the opening is larger than the diameter of the anti-convection surrounding wall, and the diameter of the sample tube is the same as the diameter of the sealing cover. The interior of the sample tube is provided with a storage cavity for storing the solvent. The sample tube is a square sample tube 12, and the storage cavity is a square sample tube inner cavity 15.

[0059] By providing the square sealing cover with the reverse arc-shaped surrounding wall 16 and the capillary hole 17 at the bottom of the square sealing cover, when the square sealing cover 11 is installed on the top of the square sample tube 12, only the area of ​​the capillary hole 17 at the bottom of the square sealing cover is left, which can reduce the air flow rate on the liquid evaporation surface to a certain extent, thereby reducing evaporation.

[0060] See attached Figure 12As shown, the two upward arrows indicate the direction of volatilization of the sample reagent, and the two inward arrows indicate the direction of flow of the volatile reagent when it is cooled. During the process of volatilization and upward movement, the volatile reagent condenses on the outer wall of the anti-arc surrounding wall 16 of the square sealing cover, and after forming liquid droplets on the outer wall of the anti-arc surrounding wall 16 of the square sealing cover, it is affected by gravity and flows along the outer wall of the anti-arc surrounding wall 16 of the square sealing cover to the position of the capillary holes at the bottom of the sealing cover. The condensed liquid droplets are continuously replenished into the capillary holes 17 at the bottom of the square sealing cover or flow back into the square sample tube 12, and part of the liquid droplets will stay in the inner cavity of the capillary holes 17 at the bottom of the square sealing cover due to capillary action due to siphoning to form a siphon liquid column of condensed liquid droplets when the volatile reagent is cooled, thereby forming a seal, which further reduces the rate of solvent volatilization. In addition, this solution has a simple structure and low cost, and solves the problem of high cost of traditional sealing structures.

[0061] Through the design of the anti-convection annular wall 21 of the inner cavity of the square sealing cover, according to Dalton's law of evaporation, the relationship between the evaporation rate of the evaporation surface and the factors affecting evaporation is W=C(Ee) / p, {W is the liquid surface evaporation rate; Ee is the saturation difference of the air, where E is the saturated air pressure at the liquid surface gas temperature, e is the actual air pressure of the air on the liquid surface; P is the air pressure; C is the proportional coefficient related to the wind speed}, the evaporation surface is proportional to the wind speed of the liquid surface, and the diameter of the inner cavity 14 of the square sealing cover is much larger than the capillary pores 17 at the bottom of the square sealing cover, wherein the anti-convection annular wall 21 of the inner cavity of the square sealing cover is mainly used to greatly reduce the air flow rate on the surface of the siphon liquid column of the volatile reagent when it encounters cold condensation droplets and the ambient air flow rate above the inner cavity 14 of the square sealing cover, thereby greatly reducing the evaporation rate of the siphon liquid column of the volatile reagent when it encounters cold condensation droplets, thereby ensuring that the sample in the inner cavity 15 of the square sample tube and the environment can be isolated and sealed for a long time.

[0062] Based on the above principle, the scheme can also be adopted by adding a certain small amount of pure water, paraffin oil or sample solution into the inner cavity 14 of the square sealing cover, so that a certain small amount of pure water, paraffin oil or sample solution will flow into the capillary holes 17 at the bottom of the square sealing cover along the inner wall of the anti-arc surrounding wall 16 of the square sealing cover under the action of gravity, and will be retained in the inner cavity of the capillary holes 17 at the bottom of the square sealing cover under the action of capillary siphon to form a volatile reagent that condenses into liquid beads and a siphon liquid column when it encounters cold, thereby isolating and sealing the sample in the inner cavity 15 of the square sample tube from the environment, further reducing volatilization.

[0063] See attached Figure 13-14 As shown, it also includes a sealing cover plate 20, and multiple sealing covers are arranged at intervals along the width direction of the sealing cover plate 20. The multiple sealing covers in the width direction form a sealing cover group, and the sealing cover group is arranged at intervals along the length direction of the sealing cover plate 20. A corresponding square sample tube 12 is provided at the bottom of each sealing cover.

[0064] A groove 18 is provided on the right side of the sealing cover 20. When the sealing cover 20 is in use, the sample tube is first installed on the bottom of the sealing cover, and then the sealing cover 20 is buckled on the storage box.

[0065] By setting the inclined groove 19 and the groove 18, the inner wall of the storage box can be corresponded to facilitate the staff to disassemble the entire sealing cover 20, and the sealing cover on the sealing cover 20 can also be processed into various quantities according to needs, such as 8-hole array combination, 48-hole array combination, 96-hole array combination or 384-hole array combination, etc., to meet the needs of different specifications.

[0066] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. It should be pointed out that ordinary technicians in this technical field can make several improvements and modifications without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A sealing structure for puncture-free sampling to prevent solvent volatilization, characterized in that: include: Sealing caps and sample tubes; The sealing cover includes a cover edge, an anti-convection surrounding wall capable of preventing gas convection, an inverted arc surrounding wall and a capillary hole, wherein the anti-convection surrounding wall is fixedly mounted on the bottom of the cover edge, the inverted arc surrounding wall is provided at the bottom of the anti-convection surrounding wall, and the capillary hole is fixedly mounted on the bottom of the inverted arc surrounding wall; The top of the sample tube is provided with an opening adapted to the sealing cover, the diameter of the opening is larger than the diameter of the anti-convection surrounding wall, and the diameter of the sample tube is the same as the diameter of the sealing cover. The interior of the sample tube is provided with a storage cavity for storing a solvent.

2. A sealing structure for preventing solvent volatilization during puncture-free sampling according to claim 1, characterized in that: The sealing cover is a circular sealing cover (1), the cover edge is a circular cover edge (3), the inner wall of the anti-arc surrounding wall is a circular anti-arc inner wall (8) of the circular sealing cover inner cavity, the outer wall of the anti-arc surrounding wall is a circular sealing cover anti-arc outer wall (6), the capillary hole is a capillary hole (7) at the bottom of the circular sealing cover, the anti-convection surrounding wall is an anti-convection inner wall (10) of the circular sealing cover inner cavity, and the anti-convection inner wall (10) of the circular sealing cover inner cavity is arranged in a circular ring shape.

3. A sealing structure for preventing solvent volatilization during puncture-free sampling according to claim 2, characterized in that: The inner wall of the capillary hole (7) at the bottom of the circular sealing cover is provided with an inner wall (9) of the capillary hole at the bottom of the circular sealing cover fixedly connected to the circular reverse arc inner wall (8) of the inner cavity of the circular sealing cover. The anti-convection inner wall (10) of the inner cavity of the circular sealing cover, the circular reverse arc inner wall (8) of the inner cavity of the circular sealing cover and the inner wall (9) of the capillary hole at the bottom of the circular sealing cover constitute the inner cavity of the circular sealing cover (4).

4. A sealing structure for preventing solvent volatilization during puncture-free sampling according to claim 3, characterized in that: The diameter of the anti-convection inner wall (10) of the circular sealing cover inner cavity is smaller than the diameter of the circular cover edge (3).

5. The sealing structure for preventing solvent volatilization during puncture-free sampling according to claim 1, characterized in that: The sample tube is a circular sample tube (2), and the storage cavity is an inner cavity (5) of the circular sample tube.

6. The sealing structure for preventing solvent volatilization during puncture-free sampling according to claim 1, characterized in that: The sealing cover is a square sealing cover (11), the cover edge is a square sealing cover edge (13), the anti-convection surrounding wall is an anti-convection annular wall (21) in the inner cavity of the square sealing cover, the inner wall of the anti-arc surrounding wall is a square sealing cover anti-arc surrounding wall (16), the square sealing cover anti-arc surrounding wall (16) is square and fixedly installed on the bottom of the square sealing cover edge (13), and the capillary hole is a capillary hole (17) at the bottom of the square sealing cover.

7. A sealing structure for preventing solvent volatilization during puncture-free sampling according to claim 6, characterized in that: The square sealing cover (11), the square sealing cover inner cavity anti-convection annular wall (21), and the inner wall of the square sealing cover surrounded by an anti-arc shape constitute a square sealing cover inner cavity (14) connected to the capillary hole (17) at the bottom of the square sealing cover.

8. The sealing structure for preventing solvent volatilization during puncture-free sampling according to claim 1, characterized in that: The sample tube is a square sample tube (12), and the storage cavity is an inner cavity (15) of the square sample tube.

9. The sealing structure for puncture-free sampling and preventing solvent volatilization according to claim 1, characterized in that: It also includes a sealing cover plate (20), wherein a plurality of sealing covers are arranged at intervals along the width direction of the sealing cover plate (20), and the plurality of sealing covers in the width direction form a sealing cover group, and a plurality of sealing cover groups are arranged at intervals along the length direction of the sealing cover plate (20), and a corresponding sample tube is provided at the bottom of each sealing cover.

10. A sealing structure for preventing solvent volatilization during puncture-free sampling according to claim 9, characterized in that: An oblique groove (19) is provided at one end of the rear side of the sealing cover plate (20), and a groove (18) is provided on the right side of the sealing cover plate (20).

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