Ultrasonic cell disruption device

Abstract

The utility model relates to an ultrasonic cell disruption device, which comprises an ultrasonic generator and a soundproof box, a lifting platform for placing a sample container is arranged in the soundproof box, a transducer penetrating from the top of the soundproof box is arranged above the lifting platform, the lower end of the transducer is connected with an amplitude transformer, and the lower part of the amplitude transformer extends into the sample container. The amplitude-change pole is sleeved with a protective sleeve with the lower end closed. The ultrasonic cell disruption device disclosed by the utility model is novel in structure, reasonable in design, convenient and practical, the amplitude-change pole can be sealed and wrapped by adding the protective sleeve on the amplitude-change pole, a sample is prevented from being in direct contact with the amplitude-change pole, a heat source is isolated, the protective sleeve can be reused, and the investment cost is low.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to an ultrasonic cell disruption device. Background Art

[0002] Ultrasonic disruptors are commonly used to disrupt cell tissues and are a common tool for extracting and recovering biological samples. Conventional ultrasonic disruptors consist of a main unit (i.e., an ultrasonic generator), a transducer, a horn, and a soundproof enclosure.

[0003] Since cells need to be kept at a low temperature, and the horn inserted into the sample container will generate heat during operation, it needs to be wrapped with tinfoil to isolate the sample from the heat source. The tinfoil wrapping method is very inconvenient to operate and cannot be reused. Utility Model Content

[0004] In view of this, the purpose of the present invention is to provide an ultrasonic cell disruption device with novel structure, convenience, practicality and low investment cost, which can avoid direct contact of the sample with the horn and isolate the heat source.

[0005] The utility model is implemented by the following scheme: an ultrasonic cell disruption device, including an ultrasonic generator and a soundproof box, a lifting platform for placing a sample container is provided in the soundproof box, a transducer is provided above the lifting platform and penetrates from the top of the soundproof box, the lower end of the transducer is connected to a horn whose lower part extends into the sample container, the outer part of the horn is provided with a protective sleeve with a closed lower end; a gap is left between the inner side wall of the lower part of the protective sleeve and the lower part of the horn.

[0006] Furthermore, a wrench hole or a wrench slot is provided on the upper side of the amplitude transformer, and a threaded hole A corresponding to the position of the wrench hole or the wrench slot is provided on the upper side of the protective sleeve, and a limit screw is provided in the threaded hole.

[0007] Furthermore, a threaded convex shaft is provided at the lower end of the transducer, and a threaded hole B is provided at the upper end of the amplitude varying rod for cooperation and connection with the threaded convex shaft.

[0008] Furthermore, an ice box is provided on the lifting platform, the sample container is placed in the ice box, and a container cover is provided above the sample container.

[0009] Furthermore, a temperature probe is provided in the sample container, the temperature probe is connected to the ultrasonic generator via a signal line, the transducer is connected to the ultrasonic generator via a cable, and the container cover is provided with through holes for the horn and the temperature probe to pass through respectively.

[0010] Furthermore, a guide rod is erected in the sound insulation box, and a guide sleeve is provided on the guide rod and can slide up and down relative to the guide rod. The lifting platform and the guide sleeve are fixedly connected together, and a locking screw is provided on the side of the guide sleeve.

[0011] Furthermore, a door is provided on the front side of the soundproof box, and sound insulation cotton is provided on the inner wall of the soundproof box and the inner side of the door.

[0012] Compared with the existing technology, the utility model has the following beneficial effects: the ultrasonic cell disruption device of the utility model has a novel structure, a reasonable design, is convenient and practical, and by adding a protective cover to the horn, the horn can be sealed and wrapped to prevent the sample from directly contacting the horn and isolating the heat source, and the protective cover can be reused, with low investment cost.

[0013] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below through specific embodiments and related drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the overall structure of an embodiment of the utility model;

[0015] Figure 2 This is a schematic diagram of the internal structure of the sound insulation box according to an embodiment of the present utility model;

[0016] Figure 3 It is a partial cross-sectional view of an embodiment of the utility model;

[0017] Explanation of the numbers in the figure: 100-ultrasonic generator, 200-soundproof box, 210-lifting platform, 220-guide rod, 230-box door, 240-guide sleeve, 250-locking screw, 300-transducer, 310-threaded cam, 400-amplitude transformer, 410-wrench hole, 500-protective cover, 510-threaded hole A, 520-limit screw, 600-ice box, 700-sample container, 710-container cover, 800-temperature probe. DETAILED DESCRIPTION

[0018] It should be noted that the following detailed descriptions are exemplary and are intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present application belongs.

[0019] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof.

[0020] like Figures 1-3As shown, an ultrasonic cell disruption device includes an ultrasonic generator 100 and a soundproof box 200. The soundproof box 200 is equipped with a lifting platform 210 for placing a sample container. Above the lifting platform is a transducer 300 that penetrates from the top of the soundproof box. The lower end of the transducer is connected to a horn 400 that extends into the sample container. The horn is covered with a protective sleeve 500 with a closed lower end. The protective sleeve is made of metal, such as stainless steel. By adding the protective sleeve 500 to the horn, the horn can be enclosed and wrapped, preventing direct contact between the sample and the horn and isolating the heat source. The protective sleeve 500 is also reusable, with low investment cost.

[0021] In this embodiment, the upper side of the horn 400 is provided with a wrench hole 410, and the upper side of the protective sleeve is provided with a threaded hole A510 corresponding to the position of the wrench hole 410, and a limit screw 520 is provided in the threaded hole; a gap is left between the inner side wall of the lower portion of the protective sleeve and the lower portion of the horn. In the specific implementation process, the horn 400 can also be provided with a wrench slot instead of a wrench hole. The functions of the two are the same, both of which are used to rotate the horn with a wrench. The protective sleeve is directly fixed using the wrench hole 410 or wrench slot on the horn, and the limit screw 520 is inserted into the wrench hole 410 or against the wrench slot to prevent the protective sleeve from falling off.

[0022] In this embodiment, a threaded protruding shaft 310 is provided at the lower end of the transducer 300, and a threaded hole B is provided at the upper end of the horn for coupling with the threaded protruding shaft. The horn is connected to the lower end of the transducer 300 via threads.

[0023] In this embodiment, an ice box 600 is provided on the lifting platform, and the sample container 700 is placed in the ice box. The ice box contains ice cubes or ice water to reduce the temperature of the sample to ensure that the sample temperature does not exceed 4°C. A container cover 710 is provided above the sample container. The addition of the container cover can seal the sample container to a certain extent and prevent the sample from being contaminated by the outside.

[0024] In this embodiment, a temperature probe 800 is provided in the sample container. The temperature probe is connected to the ultrasonic generator via a signal line, and the transducer is connected to the ultrasonic generator via a cable. The container cover is provided with through holes for the horn and the temperature probe to pass through respectively; the temperature probe 800 is used to monitor the sample temperature in real time. When the temperature exceeds the set temperature, the ultrasonic generator 100 stops working.

[0025] In this embodiment, a guide rod 220 is erected in the soundproof box, and a guide sleeve 240 is provided on the guide rod so as to slide up and down relative to the guide rod. The lifting platform and the guide sleeve are fixedly connected together, and a locking screw 250 is provided on the side of the guide sleeve.

[0026] In this embodiment, a door 230 is provided on the front side of the soundproof box, and sound insulation cotton is provided on the inner wall of the soundproof box and the inner side of the door.

[0027] Unless otherwise stated, any numerical range disclosed for any technical solution disclosed in the present invention is a preferred numerical range. Those skilled in the art should understand that a preferred numerical range is merely a numerical range that provides a more significant or representative technical effect among a wide range of practicable values. Due to the large number of numerical values, it is impossible to enumerate them exhaustively. Therefore, only some numerical values ​​are disclosed in the present invention to illustrate the technical solution of the present invention. Furthermore, the numerical values ​​listed above should not be construed as limiting the scope of protection of the present invention.

[0028] If the present invention discloses or involves components or structural parts that are fixedly connected to each other, then, unless otherwise stated, the fixed connection can be understood as: a detachable fixed connection (for example, connection using bolts or screws), and can also be understood as: a non-detachable fixed connection (for example, riveting, welding). Of course, the mutual fixed connection can also be replaced by an integrated structure (for example, manufactured by integral molding using a casting process) (except where it is obviously not possible to use an integrated molding process).

[0029] In addition, unless otherwise stated, the terms used in any technical solution disclosed in the above-mentioned utility model to express positional relationships or shapes include states or shapes that are approximate, similar or close thereto.

[0030] Any component provided by the present invention can be assembled from multiple separate components, or can be a separate component manufactured by an integral forming process.

[0031] The above description is merely a preferred embodiment of the present invention and does not constitute any limitation thereto. Any person skilled in the art may utilize the above disclosure to modify or remodel the present invention into equivalent embodiments. However, any simple modifications, equivalent variations, and modifications to the above embodiments that do not depart from the technical content of the present invention and are based on the technical essence of the present invention shall remain within the scope of protection of the present invention.

Claims

1. An ultrasonic cell disruption device, comprising an ultrasonic generator and a soundproof box, characterized in that: A lifting platform for placing sample containers is provided in the soundproof box, and a transducer is provided above the lifting platform and penetrates from the top of the soundproof box. The lower end of the transducer is connected to a variable amplitude rod whose lower part extends into the sample container, and the outside of the variable amplitude rod is provided with a protective cover with a closed lower end.

2. The ultrasonic cell disruption device according to claim 1, characterized in that: A wrench hole or a wrench slot is provided on the upper side of the amplitude transformer, and a threaded hole A corresponding to the position of the wrench hole or the wrench slot is provided on the upper side of the protective sleeve, and a limit screw is provided in the threaded hole; a gap is left between the inner side wall of the lower part of the protective sleeve and the lower part of the amplitude transformer.

3. The ultrasonic cell disruption device according to claim 2, characterized in that: The lower end of the transducer is provided with a threaded convex shaft, and the upper end of the horn is provided with a threaded hole B that is matched with the threaded convex shaft.

4. The ultrasonic cell disruption device according to claim 1, characterized in that: An ice box is provided on the lifting platform, the sample container is arranged in the ice box, and a container cover is provided above the sample container.

5. The ultrasonic cell disruption device according to claim 4, characterized in that: A temperature probe is provided in the sample container, which is connected to the ultrasonic generator via a signal line. The transducer is connected to the ultrasonic generator via a cable. The container cover is provided with through holes for the horn and the temperature probe to pass through respectively.

6. The ultrasonic cell disruption device according to claim 1, characterized in that: A guide rod is erected in the sound insulation box, and a guide sleeve is sleeved on the guide rod and can slide up and down relative to the guide rod. The lifting platform and the guide sleeve are fixedly connected together, and a locking screw is provided on the side of the guide sleeve.

7. The ultrasonic cell disruption device according to claim 1, characterized in that: The front side of the sound insulation box is provided with a box door, and the inner wall of the sound insulation box and the inner side of the box door are both provided with sound insulation cotton.

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