Antifouling auxiliary device for cell treatment

Abstract

The utility model discloses an anti-fouling auxiliary device for cell treatment, which comprises a base and a box body arranged on the base in three layers, the box body comprises an upper layer box body, a middle layer box body and a bottom layer box body, a plurality of groups of independent cover body storage boxes which are drawn forwards are arranged in the upper layer box body, and a plurality of groups of cover body storage boxes are arranged in the middle layer box body. A plurality of groups of independent cover plate storage boxes which are drawn rightwards are arranged in the middle-layer box body, and a bottle body storage box which is drawn leftwards is arranged in the bottom-layer box body; according to the device, bottle caps of multiple sets of culture bottles can be independently stored, the cleanliness of the bottle caps is guaranteed, cover plates of culture dishes can be independently stored, the sterility of the culture dishes is guaranteed, the culture dishes can be independently stored, the culture dishes are prevented from being damaged, and the service life of the culture dishes is prolonged. Meanwhile, positive pressure ventilation can be carried out on the bottle cap, the cover plate and the culture bottle, bacterium breeding is prevented, and sterility is guaranteed.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell processing, and in particular relates to an anti-fouling auxiliary device for cell processing. Background Art

[0002] Contamination is a common problem during cell culture, which can lead to abnormal cell growth, unreliable experimental results, and even failure of the entire experiment. Sources of contamination may come from microorganisms in the air, the operator's hands, culture vessels, culture medium, or experimental equipment. Common contamination problems during cell culture include bacterial contamination, fungal contamination, cross-contamination, physical contamination, and so on. Because cells are easily contaminated, aseptic operation is a key aspect of cell culture. Laboratory personnel must use aseptic techniques to manipulate cells, including using a sterile workbench, sterile utensils and culture medium, and wearing appropriate protective equipment.

[0003] The cell culture anti-fouling treatment device is based on the strict requirements of the sterile environment during the cell culture process. When performing cell manipulation in a sterile workbench, the operator needs to remove the lid of the culture dish or culture bottle before handling the cells in the vessel.

[0004] As disclosed in the utility model with authorization announcement number CN213203005U, a vessel rack for storing cell culture dishes comprises a vessel rack shell and a storage plate, wherein the storage plate is provided inside the vessel rack shell, and the storage plate is connected to the left and right side walls of the vessel rack shell by welding, a U-shaped baffle is provided on the top of the storage plate, and an air outlet is provided at the center position of the front side of the U-shaped baffle, and an air intake pipe is provided on the rear side of the vessel rack shell opposite to the air outlet, and the air intake pipe is plugged into the rear side of the vessel rack shell, and an air purification device is provided on the rear side of the air intake pipe, and the air purification device comprises a dust filter, an ultraviolet lamp, and a shell, and a shell is provided on the right side of the air intake pipe, and the shell is provided at the inlet position. A dust filter is provided on the right side of the trachea, and an ultraviolet lamp is provided on the top inner wall of the shell, which greatly reduces the probability of the culture fluid in the cell culture dish being contaminated by air bacteria. The above technical solution has the following problems: when a large number of cells need to be processed at the same time, the lids of multiple culture dishes and culture bottles that have been removed cannot be stored independently. During the operation, the operator passes objects or arms over the lids of the culture dishes and culture bottles, causing the pollution source to fall into the lids. At the same time, it is impossible to independently fix multiple culture bottles. When the pipette is used, it is easy to cause bottle mouth contamination, and it is impossible to perform positive pressure ventilation on the independently stored vessels and vessel accessories, which is easy to cause bacterial contamination of the accessories. For this reason, we propose an anti-fouling auxiliary device for cell processing. Utility Model Content

[0005] The purpose of the present utility model is to provide an anti-fouling auxiliary device for cell processing, so as to solve the problems raised in the above-mentioned background technology, that is, when a large number of cells need to be processed simultaneously, the lids of multiple culture dishes and culture bottles that have been removed cannot be stored independently; during operation, the operator passes objects or arms over the lids of the culture dishes and culture bottles, causing the anti-fouling source to fall into the lids; at the same time, it is impossible to independently fix and place multiple culture bottles; when a pipette is used, it is easy to cause bottle mouth contamination, and it is impossible to perform positive pressure ventilation on the independently stored vessels and vessel accessories, which is easy to cause bacterial contamination of the accessories.

[0006] To achieve the above-mentioned object, the present invention provides the following technical solution: an anti-fouling auxiliary device for cell processing, comprising a base and a box body arranged in three layers on the base, the box body comprising an upper box body, a middle box body, and a bottom box body, the upper box body being provided with a plurality of groups of cover storage boxes that can be independently pulled forward, the middle box body being provided with a plurality of groups of cover storage boxes that can be independently pulled rightward, and the bottom box body being provided with a bottle storage box that can be pulled rightward;

[0007] Wherein, a positive pressure air supply mechanism for ventilating the cover storage box, the cover plate storage box and the bottle storage box is further provided on the rear side of the box body.

[0008] Preferably, the cover storage box is arranged in a corresponding installation opening in the upper box body, and a first support plate and a second support plate are arranged on both sides of the cover storage box. The height of the second support plate is smaller than the height of the first support plate, so that the bottle cap can be placed at an angle, which is convenient for the operator to take the bottle cap and can reduce the occurrence of the operator's hands touching the inside of the bottle cap.

[0009] Preferably, the cover plate storage box is arranged in the installation opening of the middle box body, and the cover plate storage box is a box body structure with one side open to facilitate air circulation.

[0010] Preferably, a transverse boss and a longitudinal boss are provided in the cover storage box, and the transverse boss and the longitudinal boss are arranged in a cross shape, so that the four corners of the cover can be suspended in the air, making it convenient for the operator to take and place the cover.

[0011] Preferably, the bottle storage box is arranged in the installation opening of the bottom box body, and multiple groups of partitions are arranged in the bottle storage box. The partitions divide the box body into multiple groups of independent bottle fixing cavities, which can place culture bottles independently.

[0012] Preferably, the positive pressure air supply mechanism includes a positive pressure fan and a ventilation channel, the positive pressure fan is arranged on the base, and the ventilation channel is arranged on the rear sides of the upper box body, the middle box body and the bottom box body, the upper air inlet corresponding to the mounting port in the upper box body is arranged on the rear side of the upper box body, the middle air inlet corresponding to the mounting port in the middle box body is arranged on the rear side of the middle box body, and the lower air inlet is arranged on the rear side of the bottom box body, which can ventilate the cover storage box, the cover plate storage box and the bottle storage box.

[0013] Preferably, an air inlet is further provided on the ventilation channel, and a filter is provided in the air inlet to filter the airflow.

[0014] Preferably, a battery is also provided in the base, and the battery provides operating power for the positive pressure fan.

[0015] Compared with the prior art, the beneficial effects of the present invention are:

[0016] (1) The utility model can store the bottle caps of multiple groups of culture bottles independently, avoiding the situation where multiple groups of bottle caps are placed on the workbench and occupy space, and the bottle caps are easily contaminated during manual operation, thereby ensuring the cleanliness of the bottle caps.

[0017] (2) The utility model can store the cover of the culture dish independently, ensuring its sterility and making it easy to take it out and put it in.

[0018] (3) The utility model can place multiple groups of culture bottles independently, thus avoiding the contamination of the bottle mouth when holding the culture bottles for pipetting.

[0019] (4) The utility model can perform positive pressure ventilation on the bottle cap, cover plate and culture bottle to prevent bacterial growth and ensure sterility. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic structural diagram of the utility model from a first perspective;

[0021] Figure 2 This is a schematic diagram of the structure of the utility model from a second perspective;

[0022] Figure 3 This is a structural diagram of the cover storage box of the present invention;

[0023] Figure 4 This is a structural diagram of the cover storage box of the utility model;

[0024] Figure 5 This is a structural diagram of the bottle storage box in the utility model;

[0025] Figure 6This is a split schematic diagram of the positive pressure air supply mechanism in the utility model;

[0026] In the figure: 1. Upper box body; 2. Cover storage box; 3. Hanging buckle; 4. Middle box body; 5. Bottom box body; 6. Base; 7. Cover storage box; 8. Positive pressure air supply mechanism; 9. Bottle storage box; 10. Culture bottle; 11. Bottle cap; 21. First support plate; 22. Second support plate; 61. Charging port; 62. Battery; 71. Longitudinal boss; 72. Horizontal boss; 81. Positive pressure fan; 82. Air inlet; 83. Ventilation duct; 84. Upper air inlet; 85. Middle air inlet; 86. Lower air inlet; 91. Partition; 92. Bottle fixing cavity. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0028] See also Figure 1-Figure 3 The present invention provides a technical solution: an anti-fouling auxiliary device for cell processing, comprising a base 6 and a box arranged in three layers on the base 6, the box comprising an upper box 1, a middle box 4, and a bottom box 5. Specifically, the upper box 1, the middle box 4, and the bottom box 5 are all made of transparent plastic material, which facilitates viewing of their interiors. Multiple sets of independently pull-forward cover storage boxes 2 are arranged in the upper box 1. The cover storage boxes 2 are arranged in corresponding installation openings in the upper box 1. A first support plate 21 and a second support plate 22 are provided on both sides of the cover storage box 2. The height of the second support plate 22 is smaller than that of the first support plate 21.

[0029] When a large number of culture bottles 10 are used, the cover storage box 2 is pulled forward, and the bottle caps 11 are placed in the cover storage box 2 in sequence at an angle, and the back of the bottle caps 11 are fitted with the first support plate 21. Since the first support plate 21 is higher than the second support plate 22, a take-and-place opening is formed, which is convenient for the operator to take and place the bottle caps 11 without touching the inside of the bottle caps 11. The bottle caps 11 of multiple groups of culture bottles 10 can be stored independently, avoiding the situation where multiple groups of bottle caps 11 are placed on the workbench and occupying space, and the bottle caps 11 are easily contaminated during manual operation, thereby ensuring the cleanliness of the bottle caps 11.

[0030] As a specific embodiment of this application, please refer to Figure 4, multiple groups of cover storage boxes 7 that can be independently pulled to the right are set in the middle box body 4. The cover storage box 7 is set in the installation port of the middle box body 4. The cover storage box 7 is a box structure with one side open. The open setting is convenient for air circulation. Horizontal bosses and longitudinal bosses 71 are set in the cover storage box 7. The horizontal bosses 72 and the longitudinal bosses 71 are arranged in a cross shape.

[0031] When a large number of culture dish covers need to be stored, the cover storage box 7 is pulled to the right, and the culture dish covers are placed on the horizontal boss 72 and the longitudinal boss 71, and the four corners of the cover are suspended in the air, thereby ensuring air circulation and facilitating the taking and placing of the cover. The culture dish covers can be stored independently to ensure their sterility.

[0032] In the present application, the cover storage box 2 and the cover plate storage box 7 are both connected to the upper box 1 and the middle box 4 through door bolts, which improves their stability during storage.

[0033] As a specific embodiment of this application, please refer to Figure 5 A bottle storage box 9 that can be pulled out to the left is provided in the bottom box 5. The bottle storage box 9 is provided in the installation opening of the bottom box 5. Multiple groups of partitions 91 are provided in the bottle storage box 9. The partitions 91 divide the box into multiple groups of independent bottle fixing cavities 92, which can independently place the culture bottles 10.

[0034] Pull the bottle storage box 9 to the left, and place the culture bottles 10 independently in the bottle fixing cavity 92 one by one, and then add culture medium or intervention reagent into the culture bottle 10 through a pipette. This avoids fixing the culture bottle 10 with one hand and then adding culture fluid or intervention reagent into the culture bottle 10 with a pipette with the other hand, which will cause the hand or the unclean pipette to come into contact with the bottle mouth and inner wall of the culture bottle 10, causing cell contamination.

[0035] Specifically, four sets of hanging buckles 3 are provided on the middle box 4, which can be used to hang the pipette, saving space on the operating table, and also preventing the pipette from being placed flat on the table, causing contamination of the pipette and subsequent cell contamination.

[0036] As a specific embodiment of this application, please refer to Figure 6 A positive-pressure air supply mechanism 8 for ventilating the cover storage box 2, the cover plate storage box 7 and the bottle storage box 9 is also provided on the rear side of the box body. The positive-pressure air supply mechanism 8 includes a positive-pressure fan 81 and a ventilation channel 83. The positive-pressure fan 81 is arranged on the base 6, and the ventilation channel 83 is arranged on the rear sides of the upper box body 1, the middle box body 4 and the bottom box body 5. An upper air inlet 84 corresponding to the mounting port in the upper box body 1 is provided on the rear side of the upper box body 1, a middle air inlet 85 corresponding to the mounting port in the middle box body 4 is provided on the rear side of the middle box body 4, and a lower air inlet 86 is provided on the rear side of the bottom box body 5.

[0037] When the bottle caps 11, cover plates and culture bottles 10 are stored, the air flow is sequentially conveyed to the upper box, the middle box 4 and the bottom box 5 through the upper air inlet 82, the middle air inlet 82 and the lower air inlet 82 by the positive pressure fan 81. The air flow then passes through the mounting ports therein in sequence and is output, thereby ventilating the cap storage box 2, the cover plate storage box 7 and the bottle storage box 9 in the mounting ports, thereby ensuring the cleanliness of the stored bottle caps 11, cover plates and culture bottles 10.

[0038] Furthermore, an air inlet 82 is provided on the ventilation channel 83, and a filter is provided in the air inlet 82 to ensure the circulation and transportation of air and to filter the airflow.

[0039] Furthermore, a battery 62 is provided in the base 6, and the battery 62 provides operating power for the positive pressure fan 81. Specifically, a charging interface 61 is also provided on the base 6, and the charging interface 61 is connected to the battery 62, which can charge the battery 62. Of course, a switch for controlling the positive pressure fan 81 is also provided on the box body, which is used to control the operation of the positive pressure fan 81.

[0040] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An antifouling auxiliary device for cell processing, comprising a base (6) and a box arranged in three layers on the base (6), characterized in that: The box body comprises an upper box body (1), a middle box body (4) and a bottom box body (5); a plurality of cover storage boxes (2) that can be independently drawn forward are arranged in the upper box body (1); a plurality of cover storage boxes (7) that can be independently drawn rightward are arranged in the middle box body (4); and a bottle storage box (9) that can be drawn leftward is arranged in the bottom box body (5); The rear side of the box is also provided with a positive pressure air supply mechanism (8) for ventilating the cover storage box (2), the cover plate storage box (7) and the bottle storage box (9).

2. The anti-fouling auxiliary device for cell treatment according to claim 1, characterized in that: The cover storage box (2) is arranged in a corresponding installation opening in the upper box body (1), and a first support plate (21) and a second support plate (22) are arranged on both sides of the cover storage box (2), and the height dimension of the second support plate (22) is smaller than the height dimension of the first support plate (21).

3. The anti-fouling auxiliary device for cell treatment according to claim 1, characterized in that: The cover plate storage box (7) is arranged in the installation opening of the middle box body (4), and the cover plate storage box (7) is a box body structure with one side open.

4. The anti-fouling auxiliary device for cell treatment according to claim 3, characterized in that: A transverse boss (72) and a longitudinal boss (71) are provided in the cover plate storage box (7), and the transverse boss (72) and the longitudinal boss (71) are arranged in a cross shape.

5. The anti-fouling auxiliary device for cell treatment according to claim 1, characterized in that: The bottle storage box (9) is arranged in the installation opening of the bottom box (5), and multiple groups of partitions (91) are arranged in the bottle storage box (9), and the partitions (91) divide the box into multiple groups of independent bottle fixing cavities (92).

6. The anti-fouling auxiliary device for cell treatment according to claim 1, characterized in that: The positive pressure air supply mechanism (8) comprises a positive pressure fan (81) and a ventilation channel (83), wherein the positive pressure fan (81) is arranged on the base (6), and the ventilation channel (83) is arranged on the rear sides of the upper box (1), the middle box (4) and the bottom box (5); an upper air inlet (84) corresponding to the mounting port in the upper box (1) is arranged on the rear side of the upper box (1), a middle air inlet (85) corresponding to the mounting port in the middle box (4) is arranged on the rear side of the middle box (4), and a lower air inlet (86) corresponding to the mounting port in the bottom box (5) is arranged on the rear side of the bottom box (5).

7. The anti-fouling auxiliary device for cell treatment according to claim 6, characterized in that: An air inlet (82) is also provided on the ventilation channel (83), and a filter is provided in the air inlet (82).

8. The anti-fouling auxiliary device for cell treatment according to claim 6, characterized in that: A storage battery (62) is also provided in the base (6), and the storage battery (62) provides operating power for the positive pressure fan (81).

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