DNA extraction workstation

Abstract

The utility model discloses a DNA (deoxyribonucleic acid) extraction workstation which comprises an extraction platform, a protective cover body, a transfer chamber and a sample transfer mechanism, the protective cover body detachably covers the extraction platform and is used for forming a closed extraction environment; the transfer chamber is arranged on one side of the protective cover body and is provided with a communication port penetrating into the protective cover body, and a storage opening is formed in the side face of the transfer chamber; the sample transfer mechanism is arranged on the extraction platform and is provided with an object placing table; according to the extraction device, the extraction platform is covered with the protective cover body, so that the extraction space is sealed, and cross contamination caused by external air flow is avoided; moreover, under the arrangement of the transfer chamber and the sample moving mechanism, the sample can be automatically transferred to the extraction platform located in the protective cover body, and the communication port penetrating through the protective cover body is closed before and after the sample is transferred, so that possible internal and external cross contamination is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of DNA extraction, in particular to a DNA extraction workstation. Background Art

[0002] A DNA extraction workstation generally refers to an experimental device used to extract DNA from biological samples. For example, Chinese Patent No. 202020394099.9 discloses a micro-DNA extraction workstation, which includes a robotic arm device disposed on a workbench and capable of adding reagents from the reservoir to the lysis plate or the binding plate and shifting the lysis plate or the binding plate.

[0003] The above-mentioned existing technologies still have the following problems: particles or aerosols in the air can carry DNA fragments and spread from one area to another. The DNA of other organisms in the environment, such as skin cells of laboratory workers and dust in the laboratory, may become sources of contamination. The entire DNA extraction process is in an open state, which can easily cause cross-contamination and interfere with the DNA extraction results. Utility Model Content

[0004] The purpose of the utility model is to overcome the above technical deficiencies and propose a DNA extraction workstation to solve the technical problem in the prior art that DNA extraction is in an open state, which easily causes cross contamination and interferes with the DNA extraction results.

[0005] In order to achieve the above technical purpose, the present invention adopts the following technical solutions:

[0006] The utility model provides a DNA extraction workstation, comprising:

[0007] Extraction platform;

[0008] A protective cover, the detachable cover of which is arranged on the extraction platform and is used to form a closed extraction environment;

[0009] a transfer chamber, which is provided on one side of the protective cover and has a communication port extending into the protective cover, and a side of the transfer chamber is provided with an opening for placing items; and

[0010] The sample transfer mechanism is arranged on the extraction platform and has a storage platform thereon. The sample transfer mechanism is used to drive the storage platform to move into the transfer chamber and close the communication port, and to drive the storage platform to move onto the extraction platform and close the communication port.

[0011] In some embodiments, a sample rack is further included. The sample rack is inserted on the storage table, and sample lysis tubes are inserted in an array on the sample rack.

[0012] In some embodiments, the sample rack includes a base frame and a strip rack, the base frame is provided with a plurality of slots arranged at equal intervals, a plurality of strip racks are inserted into the slots in a one-to-one correspondence, and the sample lysis tubes are inserted into the strip racks.

[0013] In some embodiments, the sample transfer mechanism includes a linear drive member and a closing plate, the storage platform is disposed on a movable end of the linear drive member, and the closing plate is vertically disposed on top of the storage platform.

[0014] In some embodiments, a sealing ring is provided on the outer side of the closing plate.

[0015] In some embodiments, the storage opening is opened at the top of the transfer chamber and extends to one side of the transfer chamber.

[0016] In some embodiments, the extraction platform is provided with a transfer mechanism, a clamping assembly and a liquid extraction assembly. The transfer mechanism has a movable end for driving the movable end to move in the XZ plane. The clamping assembly and the liquid extraction assembly are both provided on the movable end of the transfer mechanism. The clamping assembly is used to clamp the sample rack, and the liquid extraction assembly is used to aspirate and discharge the test liquid.

[0017] In some embodiments, the extraction platform is provided with a plurality of workstations in sequence along the X-axis direction.

[0018] In some embodiments, a plurality of the workstations are sequentially provided with a lysis liquid storage tank, a heating oscillation mechanism, a centrifugal mechanism, and a magnetic rack.

[0019] In some embodiments, the magnetic rack is provided with a plurality of magnetic bars distributed in an array.

[0020] Compared with the existing technology, the DNA extraction workstation provided by the present invention is equipped with a protective cover on the extraction platform to seal the extraction space, thereby avoiding cross-contamination caused by external air flow; and, with the establishment of a transfer chamber and a sample moving mechanism, the sample can be automatically transferred to the extraction platform located in the protective cover, and the connecting port passing through the protective cover is closed before and after the transfer, thereby reducing possible internal and external cross-contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a three-dimensional schematic diagram of a DNA extraction workstation provided by an embodiment of the present utility model;

[0022] Figure 2 The utility model provides a DNA extraction workstation embodiment of the storage station is located in the transfer chamber of the top view;

[0023] Figure 3 The utility model provides a DNA extraction workstation embodiment of the storage table is located in the protective cover of the top view of the body;

[0024] Figure 4 It is a three-dimensional exploded view of a sample rack of a DNA extraction workstation provided by an embodiment of the present utility model.

[0025] Description of reference numerals:

[0026] 1. Extraction platform; 101. Transfer mechanism; 102. Clamping assembly; 103. Liquid extraction assembly; 104. Work station; 11. Lysis liquid reservoir; 12. Heating and oscillating mechanism; 13. Centrifugal mechanism; 14. Magnetic rack; 2. Protective cover; 3. Transfer chamber; 301. Connecting port; 4. Sample transfer mechanism; 401. Storage table; 41. Linear drive; 42. Closing plate; 5. Sample rack; 501. Slot; 51. Base frame; 52. Strip rack; 6. Sample lysis tube. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0028] In order to solve the technical problem that DNA extraction is in an open state, which is prone to cross infection and interferes with the DNA extraction results, the utility model provides a DNA extraction workstation that can realize closed DNA extraction and avoid cross contamination caused by the transfer of pollution sources due to external air circulation.

[0029] See also Figure 1 , Figure 1This is a structural diagram of a DNA extraction workstation in one embodiment of the present invention. The DNA extraction workstation includes an extraction platform 1, a protective cover 2, a transfer chamber 3, and a sample transfer mechanism 4. The detachable cover of the protective cover 2 is provided on the extraction platform 1 to form a closed extraction environment. The protective cover 2 is a transparent cover. Under the protection of the protective cover 2, cross contamination caused by air flow is avoided. The transfer chamber 3 is provided on one side of the protective cover 2 and is provided with a connecting port 301 that penetrates into the protective cover 2. The side of the transfer chamber 3 is provided with an opening for placing objects. The connecting port 301 is used for the sample to enter the interior of the protective cover 2 for subsequent extraction. Working; The sample transfer mechanism 4 is arranged on the extraction platform 1, and has a placement table 401 thereon. The sample transfer mechanism 4 is used to drive the placement table 401 to move into the transfer chamber 3 and close the communication port 301, and drive the placement table 401 to move onto the extraction platform 1 and close the communication port 301. When the placement table 401 is located in the transfer chamber 3, it is a position state for placing the sample. When the placement table 401 is located on the extraction platform 1, it is a position state for moving the sample to the protective cover 2. The switching of the two position states will close the communication port 301 to avoid cross-contamination of the internal extraction process by external contamination sources.

[0030] In one embodiment, see Figure 1 , further comprising a sample rack 5, which is inserted on the placement table 401, and the sample lysis tubes 6 are inserted in an array on the sample rack 5. When the placement table 401 moves, the sample rack 5 is pushed from the transfer chamber 3 to the extraction platform 1, and the sample lysis tubes 6 are transferred to the inside of the protective cover 2 to facilitate subsequent extraction work.

[0031] For further information, see Figure 4 In order to perform extraction operations on both multi-row and single-row sample lysis tubes 6, the sample rack 5 includes a base rack 51 and a strip rack 52. The base rack 51 is provided with a plurality of slots 501 arranged at equal intervals. The plurality of strip racks 52 are inserted into the slots 501 in a one-to-one correspondence. The sample lysis tubes 6 are inserted into the strip rack 52. When it is necessary to perform DNA extraction operations on samples in the sample lysis tubes 6 on the entire multi-row sample rack 5, the entire sample rack 5 is clamped. If it is necessary to perform DNA extraction operations on only a single-row sample lysis tube 6, the strip rack 52 is only clamped.

[0032] In one embodiment, see Figure 2In order to drive the placement table 401 to move and close the communication port 301 in both positions, the sample transfer mechanism 4 includes a linear drive member 41 and a closing plate 42. The placement table 401 is disposed on the movable end of the linear drive member 41, and the closing plate 42 is vertically disposed on the top of the placement table 401. The linear drive member 41 is used to drive the placement table 401 to move from the transfer chamber 3 toward the extraction platform 1, and to move the placement table 401 in and out of the communication port 301. There are two closing plates 42. When the placement table 401 passes through the communication port 301 and is located in the transfer chamber 3, one closing plate 42 is inserted into the communication port 301 for sealing; when the placement table 401 passes through the communication port 301 and is located on the extraction platform 1, the other closing plate 42 is inserted into the communication port 301 for sealing.

[0033] Furthermore, a sealing ring is provided on the outer side of the closing plate 42 , and when the closing plate 42 is inserted into the communication port 301 , the sealing ring is in contact with the inner wall of the communication port 301 for sealing.

[0034] It is understandable that the linear drive member 41 can adopt a linear guide pair, the slider on which is connected to the bottom of the storage platform 401, or other existing mature mechanisms with linear drive functions, which is not limited here.

[0035] Furthermore, in order to facilitate taking out the sample rack 5 , the storage opening is opened at the top of the transfer chamber 3 and extends to one side of the transfer chamber 3 for taking out and placing the sample rack 5 .

[0036] In one embodiment, see Figure 1 The extraction platform 1 is provided with a transfer mechanism 101, a clamping component 102 and a liquid extraction component 103. The transfer mechanism has a movable end for driving the movable end to move in the XZ plane. The clamping component 102 and the liquid extraction component 103 are both provided on the movable end of the transfer mechanism 101. The clamping component 102 is used to clamp the sample rack 5, and the liquid extraction component 103 is used to aspirate and discharge the test liquid. After the clamping component 102 clamps the sample rack 5, it can be moved under the lifting and lowering of the transfer mechanism 101, and the liquid extraction component 103 can extract the sample liquid in the sample lysis tube 6 and inject the extracted lysis liquid into the sample lysis tube 6.

[0037] Among them, the transfer mechanism 101 can adopt a robotic arm with XZ axis movement freedom to drive the clamping component 102 and the liquid extraction component 103 to move on the XZ plane for lifting, lowering and lateral movement; the clamping component 102 can adopt a clamping plate controlled by a hydraulic cylinder, and the two clamping plates respectively clamp the front and rear sides of the sample rack 5, and can be adjusted to the clamping base 51 or the strip rack 52 according to the height position of the clamping end; the liquid extraction component 103 can adopt a liquid storage box, which is connected to a suction nozzle through a suction pump and a liquid discharge pump. When the suction pump is started, it sucks and stores the liquid in the liquid storage box. When the liquid discharge pump is started, the liquid stored in the liquid storage box can be discharged from the suction nozzle.

[0038] It should be noted that the transfer mechanism 101, the clamping component 102 and the liquid extraction component 103 are just a feasible solution and are not the only limitation. Among them, the transfer mechanism 101 can form a mobile drive on the XZ plane, the clamping component 102 can clamp the sample rack 5, and the liquid extraction component 103 can suck and discharge the sample liquid.

[0039] In one embodiment, see Figure 2 and Figure 3 The extraction platform 1 is sequentially provided with a plurality of workstations 104 along the X-axis direction, wherein the plurality of workstations 104 are sequentially provided with a lysis liquid reservoir 11, a heating and oscillating mechanism 12, a centrifugal mechanism 13 and a magnetic frame 14. The magnetic frame 14 is provided with a plurality of magnetic rods distributed in an array. There are multiple lysis liquid reservoirs 11, which can be used to accumulate lysis solution, magnetic bead suspension and eluent and other liquids for extracting DNA. The heating and oscillating mechanism 12 is used to heat and oscillate the sample lysis tube 6. The centrifugal mechanism 13 is used to centrifuge the sample lysis tube 6. The magnetic rods on the magnetic frame 14 are inserted into the gaps between the lysis tubes to form magnetic adsorption for the nucleic acid.

[0040] It can be understood that the heating and oscillating mechanism 12 can adopt a mechanism with heating and oscillating functions such as a microplate heating oscillator. Specifically, its movable end has the functions of oscillating and heating. Among them, the heating and oscillating mechanism 12 can also adopt a separate heating base and oscillating mechanism. The sample rack 5 is first placed on the heating base for heating, and then moved to the oscillating mechanism for oscillation; the centrifugal mechanism 13 adopts a centrifuge, and a mounting frame matching the sample rack 5 is installed at its movable end to fix the sample rack 5 for subsequent stable centrifugation. These are all conventional operating methods of existing technologies or people in this field, and are not limited or elaborated here.

[0041] In order to better understand the present invention, the following Figures 1 to 4The technical solution of the present invention is described in detail: the sample rack 5 is placed on the storage table 401 in the transfer chamber 3, and the storage table 401 is driven by the linear drive member 41 to move to the extraction platform 1, and the closing plate 42 closes the communication port 301; then, the extraction component 103 is moved to the lysis liquid reservoir 11 by the transfer mechanism 101 to extract the lysis liquid, and then moved to the top of the sample rack 5, and the lysis liquid is injected into the sample lysis tube 6; then, the clamping component 102 is moved by the transfer mechanism 101, and the sample rack 5 is clamped by the clamping component 102, and then moved to the heating and oscillation mechanism 12, the clamping is released, and the heating and oscillation mechanism 12 is used to heat and oscillate; thereafter, the clamping component 102 is moved again by the transfer mechanism 101. , and the sample rack 5 is clamped by the clamping component 102, and then moved to the centrifugal mechanism 13 for centrifugation; the magnetic bead suspension is then extracted by the extraction component 103 and injected into the sample lysis tube 6; the clamping component 102 is moved by the transfer mechanism 101, and the sample rack 5 is clamped by the clamping component 102, and then moved to the magnetic rack 14, magnetically adsorbing the nucleic acid on the inner wall of the sample lysis tube 6, and then the residual test solution is transferred out of the sample lysis tube 6 by the extraction component 103, and then the eluent is injected to wash the nucleic acid out of the magnetic beads, and then extracted and placed in an empty sample lysis tube 6, thus completing the nucleic acid extraction action, that is, the magnetic bead method for extracting nucleic acids; the sample rack 5 is then clamped and moved to the storage table 401, and pushed out into the transfer chamber 3.

[0042] The entire workflow is complete, and all contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field.

[0043] The specific embodiments of the present invention described above do not limit the scope of protection of the present invention. Any other corresponding changes and modifications made based on the technical concept of the present invention should be included in the scope of protection of the claims of the present invention.

Claims

1. A DNA extraction workstation, characterized in that: include: Extraction platform; A protective cover, the detachable cover of which is arranged on the extraction platform and is used to form a closed extraction environment; A transfer chamber is provided on one side of the protective cover and is provided with a communication port extending into the protective cover, and a storage opening is provided on the side of the transfer chamber; as well as The sample transfer mechanism is arranged on the extraction platform and has a storage platform thereon. The sample transfer mechanism is used to drive the storage platform to move into the transfer chamber and close the communication port, and to drive the storage platform to move onto the extraction platform and close the communication port.

2. The DNA extraction workstation according to claim 1, characterized in that The device also includes a sample rack, which is inserted on the storage table and has sample lysis tubes inserted in an array.

3. The DNA extraction workstation according to claim 2, characterized in that The sample rack comprises a base frame and a strip frame. The base frame is provided with a plurality of slots arranged at equal intervals. The plurality of strip frames are inserted into the slots in a one-to-one correspondence, and the sample lysis tubes are inserted into the strip frames.

4. The DNA extraction workstation according to claim 3, characterized in that: The sample transfer mechanism includes a linear drive member and a closing plate. The storage platform is arranged on the movable end of the linear drive member, and the closing plate is vertically arranged on the top of the storage platform.

5. The DNA extraction workstation according to claim 4, characterized in that: A sealing ring is provided on the outer side of the closing plate.

6. The DNA extraction workstation according to claim 5, characterized in that: The storage opening is opened at the top of the transfer chamber and extends to one side of the transfer chamber.

7. The DNA extraction workstation according to claim 6, characterized in that: The extraction platform is provided with a transfer mechanism, a clamping assembly and a liquid extraction assembly. The transfer mechanism has a movable end for driving the movable end to move in the XZ plane. The clamping assembly and the liquid extraction assembly are both provided on the movable end of the transfer mechanism. The clamping assembly is used to clamp the sample rack, and the liquid extraction assembly is used to aspirate and discharge the test liquid.

8. The DNA extraction workstation according to claim 7, characterized in that: The extraction platform is sequentially provided with a plurality of workstations along the X-axis direction.

9. The DNA extraction workstation according to claim 8, characterized in that: The plurality of workstations are sequentially provided with a cracking liquid storage tank, a heating oscillation mechanism, a centrifugal mechanism and a magnetic frame.

10. The DNA extraction workstation according to claim 9, characterized in that: The magnetic frame is provided with a plurality of magnetic bars distributed in an array.

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