Liquid changing device of bioreactor
By using a combined structure of liquid outlet tube and filter in a bioreactor, combined with the use of liquid inlet tube and pump, the problem of loss of microcarriers during liquid replacement is solved, efficient liquid replacement and stable liquid level management are achieved, and the success rate of microcarrier culture is improved.
Patent Information
- Application Number
- CN202422427641.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-08
- Publication Date
- 2025-08-26
- Estimated Expiration
- 2034-10-08
AI Technical Summary
In the perfusion culture mode of the bioreactor, the microcarrier is easily exported with the culture medium during the liquid change process, resulting in a decrease in the number of microcarriers and affecting the culture effect.
The combined structure of the liquid outlet tube and the filter is adopted. The culture medium is extracted through the liquid outlet tube, and the filter retains the microcarrier. Combined with the use of the liquid inlet tube and the pump, the liquid level is maintained, ensuring that new culture medium enters, and reducing the discharge of the microcarrier with the culture medium.
It improves the liquid change efficiency, reduces the loss of microcarriers, stabilizes the liquid level, and improves the success rate of microcarriers culture.
Smart Images
Figure CN223268665U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of microcarrier culture, and in particular to a liquid exchange device for a bioreactor. Background Art
[0002] In the biopharmaceutical industry, bioreactors are a commonly used cell culture equipment suitable for large-scale cell culture. Common culture modes include batch culture, perfusion culture, and supplementary culture.
[0003] In perfusion culture mode, bioreactors require daily culture medium replacement, requiring both inflow and outflow, maintaining a constant total volume of culture medium. Currently, the primary method used is natural microcarrier sedimentation, where a settling device separates the microcarriers from the culture medium, which is then drained out. However, during natural microcarrier sedimentation, some microcarriers fail to settle and are drained out with the culture medium, resulting in a reduction in the microcarrier population and culture failure. Utility Model Content
[0004] In order to reduce the adverse effects of liquid replacement on microcarrier culture, the present application provides a liquid replacement device for a bioreactor.
[0005] The liquid replacement device of a bioreactor provided in this application adopts the following technical solution:
[0006] A liquid exchange device for a bioreactor comprises a bottle body and a cover body adapted to the bottle body, wherein a liquid outlet pipe is provided in the bottle body, the lower end of which contacts the liquid surface in the bottle body, the upper end of the liquid outlet pipe passes through the cover body, a plurality of liquid outlet holes communicating with the interior of the liquid outlet pipe are opened on the side wall of the lower end of the liquid outlet pipe, the lower end of the liquid outlet pipe is blocked and sleeved with a cylindrical filter screen that blocks the liquid outlet holes, and a liquid inlet pipe communicating with the interior of the bottle body is plugged into the cover body.
[0007] By adopting the above technical solution, the culture medium in the bottle is extracted through the liquid outlet pipe. At this time, the culture medium passes through the filter screen and the liquid outlet hole and is discharged through the liquid outlet pipe. At this time, the filter screen intercepts the microcarriers, reducing the possibility of the microcarriers moving with the culture medium and being discharged from the bottle. At this time, new culture medium enters the bottle through the liquid inlet pipe, thereby improving the liquid exchange efficiency and reducing the adverse effects of liquid exchange on microcarrier culture.
[0008] Optionally, a liquid outlet pump is installed at one end of the liquid outlet pipe passing through the cover body, and a liquid inlet pump is installed at one end of the liquid inlet pipe away from the bottle body.
[0009] By adopting the above technical solution, the liquid outlet pump discharges the culture medium in the bottle through the liquid outlet pipe, and the liquid inlet pump delivers new culture medium to the bottle through the liquid inlet pipe. At this time, the liquid inlet pump and the liquid outlet pump make the liquid level in the bottle always maintained at the lower end of the liquid outlet pipe, and the culture medium in the bottle can always enter the liquid outlet pipe through the liquid outlet hole, thereby facilitating liquid replacement, while reducing the possibility of microcarriers being discharged with the culture medium, and reducing the adverse effects of liquid replacement on microcarrier culture.
[0010] Optionally, a through hole is opened on the cover body, a sealing gasket is provided in the through hole, the upper end of the liquid outlet pipe passes through the through hole, and the sealing gasket is sleeved on the upper end of the liquid outlet pipe to limit the liquid outlet pipe.
[0011] By adopting the above technical solution, the cover body limits the liquid outlet tube through the sealing gasket, thereby improving the stability of the liquid outlet tube in discharging the culture medium. At the same time, it is convenient to adjust the length of the upper end of the liquid outlet tube passing through the cover body according to the liquid level height in the bottle body, so that the lower end of the liquid outlet tube is always in contact with the liquid level in the bottle body, which is convenient for liquid replacement and reduces the adverse effects of liquid replacement on microcarrier culture.
[0012] Optionally, the through hole is configured as a threaded hole, a limiting cylinder is inserted into the through hole and threadedly connected, and the sealing gasket is inserted into the limiting cylinder and fixedly connected to the inner side wall of the limiting cylinder.
[0013] By adopting the above technical solution, the limiting cylinder is threadedly connected to the cover body through the threaded hole. At this time, the upper end of the liquid outlet pipe passes through the middle of the limiting cylinder and contacts the side wall of the sealing gasket, so that the relative position of the liquid outlet pipe is fixed by the friction between the sealing gasket and the side wall of the liquid outlet pipe, which is convenient for adjusting the height of the liquid outlet pipe relative to the bottle body, and at the same time convenient for disassembly and replacement of a new liquid outlet pipe, thereby improving work efficiency.
[0014] Optionally, the filter is welded to the outer side of the lower end of the liquid outlet pipe.
[0015] By adopting the above technical solution, the filter is installed on the liquid outlet pipe by welding, which improves the stability of the filter and the liquid outlet pipe, reduces the possibility of the filter falling off, and reduces the adverse effects of liquid change on microcarrier culture.
[0016] Optionally, the lower end of the liquid outlet pipe is sleeved and fixedly connected to a limiting ring, the filter is sleeved on the lower end of the liquid outlet pipe and one end of the filter is in contact with the limiting ring, and a fixing part is provided on the liquid outlet pipe to fix one end of the filter away from the limiting ring.
[0017] By adopting the above technical solution, the filter is sleeved on the liquid outlet pipe until the end of the filter contacts the side wall of the limit ring. At this time, the end of the filter away from the limit ring is fixed by the fixing part, thereby facilitating the removal and installation of the filter and improving the replacement efficiency.
[0018] Optionally, the fixing member includes an iron wire wound around the outer side of one end of the filter away from the limiting ring, and the circular ring formed by the winding of the iron wire is interference fit with the liquid outlet pipe.
[0019] By adopting the above technical solution, the end of the filter screen away from the limiting ring is fixed to the liquid outlet pipe by the iron wire, which facilitates the removal and installation of the filter screen and improves the replacement efficiency.
[0020] Optionally, the fixing member includes a fixing cylinder located at one end of the filter away from the limiting ring, the fixing cylinder is sleeved on the outside of the liquid outlet pipe and threadedly connected to the liquid outlet pipe, and the end of the filter away from the limiting ring is in contact with the side wall of the fixing cylinder.
[0021] By adopting the above technical solution, the filter is sleeved on the liquid outlet pipe until the end of the filter contacts the side wall of the limiting ring. At this time, the fixed cylinder is sleeved on the liquid outlet pipe and driven to rotate until the side wall of the fixed cylinder abuts against the side of the filter away from the limiting ring, thereby facilitating the removal and installation of the filter and improving replacement efficiency.
[0022] In summary, this application includes at least one of the following beneficial technical effects:
[0023] 1. The culture medium in the bottle is extracted through the liquid outlet tube. At this time, the culture medium passes through the filter and the liquid outlet hole and is discharged through the liquid outlet tube. At this time, the filter retains the microcarriers, reducing the possibility of the microcarriers moving with the culture medium and being discharged from the bottle. At this time, new culture medium enters the bottle through the liquid inlet tube, thereby improving the efficiency of liquid exchange and reducing the adverse effects of liquid exchange on microcarrier culture.
[0024] 2. The outlet pump discharges the culture medium in the bottle through the outlet tube, and the inlet pump delivers new culture medium into the bottle through the inlet tube. At this time, the inlet pump and the outlet pump always keep the liquid level in the bottle at the lower end of the outlet tube, and the culture medium in the bottle can always enter the outlet tube through the outlet hole, thereby facilitating liquid replacement and reducing the possibility of microcarriers being discharged with the culture medium, thereby reducing the adverse effects of liquid replacement on microcarrier culture;
[0025] 3. Slide the filter onto the liquid outlet pipe until the end of the filter contacts the side wall of the limit ring. At this time, fix the end of the filter away from the limit ring with the fixing parts, so as to facilitate the removal and installation of the filter and improve the replacement efficiency. BRIEF DESCRIPTION OF THE DRAWINGS
[0026] Figure 1 It is a schematic diagram of the overall structure of the liquid exchange device of the bioreactor in Example 1 of the present application.
[0027] Figure 2 It is a structural diagram showing the positional relationship between the liquid inlet pipe and the liquid outlet pipe in Example 1 of the present application.
[0028] Figure 3It is a structural diagram showing the positional relationship between the liquid pipe and the fixing member in Example 2 of the present application.
[0029] Figure 4 It is a structural diagram showing the positional relationship between the liquid tube and the fixed cylinder in Example 3 of the present application.
[0030] Figure 5 It is a structural diagram showing the positional relationship between the filter screen and the fixed cylinder in Example 3 of the present application.
[0031] Explanation of the accompanying symbols: 1. Bottle body; 2. Cover body; 21. Through hole; 3. Liquid outlet pipe; 31. Liquid outlet hole; 32. First connecting pipe; 33. Liquid outlet pump; 34. Limiting ring; 4. Filter; 5. Limiting cylinder; 51. Sealing gasket; 6. Liquid inlet pipe; 61. Second connecting pipe; 62. Liquid inlet pump; 7. Fixing part; 71. Iron wire; 72. Fixing cylinder. DETAILED DESCRIPTION
[0032] The present application is further described in detail below with reference to the accompanying drawings.
[0033] The embodiments of the present application disclose a liquid replacement device for a bioreactor.
[0034] Example 1
[0035] Reference Figure 1 and Figure 2 A liquid exchange device for a bioreactor includes a bottle body 1 with an upward opening, the upper end of which is covered by a horizontal cover 2. A vertical liquid outlet pipe 3 is provided on one side of the upper end of the bottle body 1. The lower end of the liquid outlet pipe 3 is inserted into the bottle body 1 and bends horizontally in a direction away from the rotation axis of the bottle body 1. The bent end of the liquid outlet pipe 3 is blocked, and a plurality of evenly distributed liquid outlet holes 31 are formed around the bent end of the liquid outlet pipe 3.
[0036] A cylindrical filter screen 4 is sleeved on one bent end of the liquid outlet pipe 3. The filter screen 4 contacts the side wall of the bent end of the liquid outlet pipe 3, and the end of the filter screen 4 is welded to the outer wall of the liquid storage tank. In the embodiment of the present application, the filter screen 4 is set as a 200-mesh stainless steel filter screen 4.
[0037] The cover 2 has a through hole 21, into which a limiting cylinder 5 is inserted. In the embodiment of the present application, the through hole 21 is configured as a threaded hole, and the limiting cylinder 5 is threadedly connected to the cover 2 via the threaded hole. A sealing gasket 51 is inserted and fixedly connected to the limiting cylinder 5. The upper end of the liquid outlet tube 3 passes through the middle of the limiting cylinder 5, and the sealing gasket 51 is sleeved on the outer side of the upper end of the liquid outlet tube 3.
[0038] Reference Figure 1 and Figure 2The upper end of the liquid outlet pipe 3 is fixedly connected and communicated with a first connecting pipe 32, and a liquid outlet pump 33 is installed on the first connecting pipe 32. A vertical liquid inlet pipe 6 that communicates with the interior of the bottle body 1 is inserted and fixedly connected to the side of the cover body 2 away from the liquid outlet pipe 3. The upper end of the liquid inlet pipe 6 is fixedly connected and communicated with a second connecting pipe 61, and a liquid inlet pump 62 is installed on the second connecting pipe 61.
[0039] The liquid outlet pipe 3 is driven by the liquid outlet pump 33 and the first connecting pipe 32 to extract the culture medium in the bottle body 1. At this time, the culture medium passes through the filter screen 4 and the liquid outlet hole 31 and is discharged through the liquid outlet pipe 3. The filter screen 4 intercepts the microcarriers, reducing the possibility of the microcarriers moving with the culture medium and being discharged from the bottle body 1. At this time, the new culture medium enters the bottle body 1 through the second connecting pipe 61 and the liquid inlet pump 62 through the liquid inlet pipe 6 until the liquid replacement is completed.
[0040] The inlet pump 62 and outlet pump 33 maintain the liquid level in bottle 1 at the lower end of outlet tube 3. Culture medium in bottle 1 can always enter outlet tube 3 through outlet hole 31, facilitating liquid replacement. Filter screen 4 is welded to outlet tube 3, improving the stability of the filter screen 4 and outlet tube 3 and reducing the possibility of filter screen 4 falling off.
[0041] The cover body 2 limits the liquid outlet tube 3 through the sealing gasket 51, thereby improving the stability of the liquid outlet tube 3 in discharging the culture medium, and at the same time facilitating the adjustment of the length of the upper end of the liquid outlet tube 3 passing through the cover body 2 according to the height of the liquid level in the bottle body 1, so that the lower end of the liquid outlet tube 3 is always in contact with the liquid level in the bottle body 1; the limiting cylinder 5 is threadedly connected to the cover body 2 through a threaded hole, and at this time, the upper end of the liquid outlet tube 3 passes through the middle of the limiting cylinder 5 and contacts the side wall of the sealing gasket 51, thereby achieving the fixation of the relative position of the liquid outlet tube 3 through the friction between the sealing gasket 51 and the side wall of the liquid outlet tube 3, and facilitating the disassembly and replacement of a new liquid outlet tube 3.
[0042] The implementation principle of Example 1 is as follows: the liquid outlet pipe 3 is driven by the liquid outlet pump 33 and the first connecting pipe 32 to extract the culture medium in the bottle body 1. At this time, the culture medium passes through the filter screen 4 and the liquid outlet hole 31 and is discharged through the liquid outlet pipe 3. The filter screen 4 intercepts the microcarriers, reducing the possibility of the microcarriers moving with the culture medium and being discharged from the bottle body 1. At this time, the new culture medium enters the bottle body 1 through the second connecting pipe 61 and the liquid inlet pump 62 through the liquid inlet pipe 6 until the liquid replacement is completed.
[0043] Example 2
[0044] Reference Figure 3 The difference between this embodiment and embodiment 1 is that the bent end of the liquid outlet pipe 3 and the end away from the sealed end is sleeved and fixedly connected to the limit ring 34, the filter screen 4 is sleeved on the outside of the bent end of the liquid outlet pipe 3, one end of the filter screen 4 is in contact with the side wall of the limit ring 34, and the bent end of the liquid outlet pipe 3 is provided with a fixing part 7 for fixing the end of the filter screen 4 away from the limit ring 34.
[0045] The fixing member 7 includes an iron wire 71 wound around the outer side of the end of the filter screen 4 away from the limiting ring 34 . The circular ring formed by the wound iron wire 71 is interference fit with the liquid outlet pipe 3 .
[0046] The implementation principle of Example 2 is: the filter screen 4 is put on the liquid outlet pipe 3 until the end of the filter screen 4 contacts the side wall of the limiting ring 34. At this time, the iron wire 71 is wrapped around the outer side of the end of the filter screen 4 away from the limiting ring 34, so that the end of the filter screen 4 away from the limiting ring 34 is fixed to the liquid outlet pipe 3 through the iron wire 71, which is convenient for disassembly and installation of the filter screen 4.
[0047] Example 3
[0048] Reference Figure 4 and Figure 5 The difference between this embodiment and embodiment 2 is that the fixing member 7 includes a fixing cylinder 72 that is sleeved on one end of the liquid outlet pipe 3 and threadedly connected to the outer wall of the liquid outlet pipe 3. When the fixing cylinder 72 is fixed on the liquid outlet pipe 3, the side wall of the fixing cylinder 72 contacts the end of the filter screen 4 away from the limiting ring 34.
[0049] The implementation principle of Example 3 is as follows: the filter screen 4 is sleeved on the liquid outlet pipe 3 until the end of the filter screen 4 contacts the side wall of the limiting ring 34. At this time, the fixing cylinder 72 is sleeved on the liquid outlet pipe 3 and the fixing cylinder 72 is driven to rotate until the side wall of the fixing cylinder 72 abuts against the side of the filter screen 4 away from the limiting ring 34, thereby completing the installation and fixation of the filter screen 4.
[0050] The above are all preferred embodiments of the present application, and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.
Claims
1. A liquid replacement device for a bioreactor, characterized in that: The invention comprises a bottle body (1) and a cover body (2) adapted to the bottle body (1); a liquid outlet pipe (3) whose lower end contacts the liquid surface in the bottle body (1) is provided in the bottle body (1); the upper end of the liquid outlet pipe (3) passes through the cover body (2); a plurality of liquid outlet holes (31) communicating with the interior of the liquid outlet pipe (3) are provided on the side wall of the lower end of the liquid outlet pipe (3); the lower end of the liquid outlet pipe (3) is blocked and sleeved with a cylindrical filter (4) blocking the liquid outlet holes (31); and a liquid inlet pipe (6) communicating with the interior of the bottle body (1) is plugged into the cover body (2).
2. The liquid replacement device for a bioreactor according to claim 1, characterized in that: An outlet pump (33) is installed at one end of the liquid outlet pipe (3) passing through the cover body (2), and an inlet pump (62) is installed at one end of the liquid inlet pipe (6) away from the bottle body (1).
3. The liquid replacement device for a bioreactor according to claim 2, characterized in that: The cover body (2) is provided with a through hole (21), a sealing gasket (51) is provided in the through hole (21), the upper end of the liquid outlet pipe (3) passes through the through hole (21), and the sealing gasket (51) is sleeved on the upper end of the liquid outlet pipe (3) and limits the position of the liquid outlet pipe (3).
4. The liquid replacement device for a bioreactor according to claim 3, characterized in that: The through hole (21) is configured as a threaded hole, the limiting cylinder (5) is inserted into the through hole (21) and threadedly connected, and the sealing gasket (51) is inserted into the limiting cylinder (5) and fixedly connected to the inner side wall of the limiting cylinder (5).
5. The liquid replacement device for a bioreactor according to claim 1, characterized in that: The filter screen (4) is welded to the outside of the lower end of the liquid outlet pipe (3).
6. The liquid replacement device for a bioreactor according to claim 1, characterized in that: The lower end of the liquid outlet pipe (3) is sleeved and fixedly connected to a limiting ring (34), the filter screen (4) is sleeved on the lower end of the liquid outlet pipe (3) and one end of the filter screen (4) is in contact with the limiting ring (34), and a fixing member (7) is provided on the liquid outlet pipe (3) to fix one end of the filter screen (4) away from the limiting ring (34).
7. The liquid replacement device for a bioreactor according to claim 6, characterized in that: The fixing member (7) comprises an iron wire (71) wound around the outside of one end of the filter screen (4) away from the limiting ring (34), and the circular ring formed by the winding of the iron wire (71) is interference-fitted with the liquid outlet pipe (3).
8. The liquid replacement device for a bioreactor according to claim 6, characterized in that: The fixing member (7) includes a fixing cylinder (72) located at one end of the filter screen (4) away from the limiting ring (34), the fixing cylinder (72) is sleeved on the outside of the liquid outlet pipe (3) and is threadedly connected to the liquid outlet pipe (3), and the end of the filter screen (4) away from the limiting ring (34) contacts the side wall of the fixing cylinder (72).