Heart section dyeing device
By designing a cardiac slice dyeing device, the problems of bulge and wrinkle in the staining process of cardiac slices are solved, and the flatness and morphology of the slices are maintained, which improves the accuracy and experimental efficiency of infarct area analysis.
Patent Information
- Application Number
- CN202422467463.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-12
- Publication Date
- 2025-08-26
- Estimated Expiration
- 2034-10-12
AI Technical Summary
Heart sections are prone to bulge and wrinkle during the staining process, resulting in uneven sections and affecting the accuracy of the infarct area analysis.
Design a cardiac slice dyeing device, including a staining upper plate and a staining lower plate, set up a slice incubation chamber and a liquid path, ensure that the slice incubation chamber is sealed and connected to the liquid path, and add the dye liquid through the reagent well to avoid the slice turning, providing an independent staining space and environment.
Maintain the flatness and morphology of the sections, improve the accuracy of the calculation of the area of the infarct area, reduce operation steps and time, reduce tissue damage, and improve experimental efficiency and result accuracy.
Smart Images
Figure CN223272272U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of biomedicine, in particular to a heart slice staining device. Background Art
[0002] TTC (2,3,5-triphenyltetrazolium chloride) staining is a procedure used in tissue damage analysis. It differentiates between infarcted and non-infarcted areas of the myocardium by utilizing the principle that TTC reacts with succinate dehydrogenase in the mitochondria of living cells, producing a red color. The non-infarcted area contains living cells and is stained red; the infarcted area, due to cell damage or death, is not stained and appears white. After staining, the severity of myocardial damage is determined by analyzing the size of the white infarcted area. TTC staining plays a key role in the analysis of hypoxic-ischemic damage in brain, heart, and kidney tissues and is one of the most commonly used laboratory techniques.
[0003] In the current staining process, there are no specific requirements for the container in which the slices are stained; the general procedure is to immerse the slices in the dye solution. However, there are significant differences in the staining effects of slices from different tissues. The most direct reason is that the structure and composition of slices from different organs vary significantly. For example, brain tissue is mainly composed of neurons and glial cells, while myocardial tissue is mainly composed of fibroblasts, cardiomyocytes, endothelial cells, and smooth muscle cells. Among them, cardiomyocytes are cells with significant contractile properties. Therefore, brain tissue slices can maintain a good morphology during the staining process. However, heart slices often show internal bulges and tissue shrinkage during the staining process. The unevenness of the heart slices leads to significant differences in cross-sectional area before and after staining. This not only makes it impossible to show the original appearance of the slice, but also increases the difficulty of analyzing the infarct area, resulting in inaccurate experimental results. Utility Model Content
[0004] Based on the above technical problems, the utility model proposes a heart slice staining device.
[0005] The technical solution adopted by this utility model is:
[0006] A heart slice staining device comprises an upper staining plate and a lower staining plate, wherein the upper staining plate is placed in the lower staining plate, and the bottom side of the upper staining plate is sealed and fitted with the top side of the bottom of the lower staining plate;
[0007] A slice incubation chamber and a liquid path are provided at the bottom of the staining upper plate, the slice incubation chamber and the liquid path are connected, and a reagent hole is provided at the end of the liquid path;
[0008] The slice incubation chamber and the liquid path are both formed by the bottom side of the staining upper plate protruding upward, the longitudinal cross section is groove-shaped, and is open at the bottom side of the staining upper plate;
[0009] When the upper staining dish is placed in the lower staining dish, the openings of the slice incubation chamber and the liquid path are blocked by the top side of the bottom of the lower staining dish.
[0010] Preferably, each liquid path is connected to a plurality of slice incubation chambers.
[0011] Preferably, there are two liquid circuits, namely the first liquid circuit and the second liquid circuit. The first liquid circuit and the second liquid circuit are arranged vertically crosswise to separate four sample areas. The first liquid circuit and the second liquid circuit are respectively connected to three slice incubation chambers corresponding to each sample area, and all slice incubation chambers are arranged along the length direction of the first liquid circuit or the second liquid circuit.
[0012] Preferably, a recording area is provided in each sample area.
[0013] Preferably, the reagent wells are arranged on the side of the staining upper plate.
[0014] Preferably, the transverse cross-section of the slice incubation chamber is arc-shaped, and the slice incubation chamber is connected to the liquid path at the arc-shaped notch.
[0015] Preferably, the upper dyeing plate and the lower dyeing plate are both disc-shaped, the top of the lower dyeing plate is open and the inside is hollow; the inner diameter of the lower dyeing plate is 1.2-1.5 times the outer diameter of the upper dyeing plate, and the height of the upper dyeing plate is higher than that of the lower dyeing plate.
[0016] Preferably, the dyeing upper plate is made of transparent material.
[0017] The beneficial technical effects of the utility model are:
[0018] (1) The cardiac slice staining device of the present invention utilizes an upper staining plate and a lower staining plate, and sets a slice incubation chamber on the upper staining plate according to the size and thickness of conventional cardiac slices, thereby creating a specific staining space for each cardiac slice to solve the problem of tissue bulging during the staining process. The present invention can avoid the bulging of the cut surface caused by tissue shrinkage, while maintaining the original flatness and shape of the cut surface. At the same time, it ensures the visual effect of the cardiac slice presentation and more effectively improves the accuracy of the calculation of the infarct area.
[0019] (2) The present invention is provided with reagent holes and liquid channels, which can add or replenish the required reagents during the staining process as needed without turning the slices. This not only reduces the number of operating steps and saves a lot of time, but also protects the slices from operating damage, effectively ensuring the perfect presentation of the slice morphology.
[0020] (3) The present invention is provided with multiple sample areas, which can stain multiple hearts simultaneously. One sample area is provided with multiple slice incubation chambers, such as 6 or 8, which can also meet the need for multiple continuous slices of a heart.
[0021] (4) The present invention has a wide range of applications, particularly for tissue sections with contractile properties, such as the heart. This ensures the presentation of the tissue section while reducing sample loss due to tissue shrinkage. Of course, the present invention is not limited to staining cardiac sections, but is also applicable to staining other non-contractile tissues of conventional thickness and size.
[0022] (5) The present invention can ensure the perfect presentation of cardiac slices and the accuracy of experimental results, which will greatly improve experimental efficiency, reduce a large number of repetitive operations in basic research, save tissue samples and reduce financial investment, and effectively guarantee the advancement of related research. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 This is a schematic diagram of the overall structure of the cardiac slice staining device of the present invention;
[0024] Figure 2 This is a schematic diagram of the structural principle of the dyeing upper plate in the dyeing device of the present invention;
[0025] Figure 3 for Figure 2 Bottom view of the .
[0026] In the figure: 1-staining upper plate, 2-staining lower plate, 3-bottom of staining upper plate, 4-bottom of staining lower plate, 5-slice incubation chamber, 6-liquid path, 7-reagent well, 8-sample area, 9-recording area. DETAILED DESCRIPTION
[0027] like Figure 1-3As shown, a cardiac slice staining device comprises a staining upper plate 1 and a staining lower plate 2, wherein the staining upper plate 1 is placed in the staining lower plate 2, and the bottom side of the staining upper plate bottom 3 is sealed and fitted with the top side of the staining lower plate bottom 4. A slice incubation chamber 5 and a liquid path 6 are provided at the bottom of the staining upper plate, and the slice incubation chamber 5 and the liquid path 6 are connected, and a reagent hole 7 is provided at the end of the liquid path 6. The slice incubation chamber 5 and the liquid path 6 are both formed by the bottom side of the staining upper plate bottom bulging upward or bulging upward, and the longitudinal cross-section is groove-shaped and open at the bottom side of the staining upper plate bottom. When the staining upper plate 1 is placed in the staining lower plate 2, the opening of the slice incubation chamber 5 and the liquid path 6 is blocked by the top side of the staining lower plate bottom. That is to say, the bottom of the staining upper plate 1 needs to have a certain thickness to facilitate the opening of a strip groove on its bottom side to form the liquid path 6, and an arc groove connected to the strip groove is opened on one side of the strip groove to form the slice incubation chamber 5. In this way, when the upper staining tray 1 is placed inside the lower staining tray 2 and the bottom side of the upper staining tray is sealed against the top side of the bottom of the lower staining tray, the open portion of the slice incubation chamber 5 and the liquid path 6 are sealed. The reagent wells 7 are provided at the bottom corners of the side of the upper staining tray 1. At this time, dye and other reagents added through the reagent wells can be transported to the slice incubation chamber 5 through the liquid path 6 to stain or fix the heart slices placed in the slice incubation chamber 5.
[0028] The slice incubation chamber 5 has an arc-shaped transverse cross-section to accommodate cardiac slices, etc. The slice incubation chamber is connected to the liquid path at a notch in the arc, facilitating the flow of dye, etc., from the liquid path 6, into the slice incubation chamber 5. The height, or depth, of the slice incubation chamber 5 is generally set to 1-2 mm, preferably slightly greater than the thickness of the cardiac slice. Specifically, the height of the slice incubation chamber can be designed to be 1.01-1.05 times the thickness of the cardiac slice.
[0029] The utility model is used for staining heart slices, which can effectively avoid the slices from bulging, keep the cross section flat, present the original cross section of the tissue, accurately obtain experimental results, reduce the difficulty of operation and save experimental costs.
[0030] Furthermore, there are two liquid circuits, namely the first liquid circuit and the second liquid circuit, which are arranged vertically and cross each other to separate four sample areas 8. The first liquid circuit and the second liquid circuit are connected to three slice incubation chambers corresponding to each sample area, and all slice incubation chambers are arranged along the length direction of the first liquid circuit or the second liquid circuit. Figure 3 As shown, each sample area 8 is provided with a recording area 9.
[0031] Furthermore, both the upper and lower staining trays 1 and 2 are disc-shaped, with the lower staining tray 2 open at the top and hollow inside. The inner diameter of the lower staining tray 2 is 1.2-1.5 times the outer diameter of the upper staining tray 1. This allows the upper staining tray 1 to fit within the lower staining tray 2, making it convenient to inject or aspirate dye from the reagent wells 7. The upper staining tray 1 is taller than the lower staining tray 2 to facilitate removal.
[0032] In addition, the staining plate 1 is made of transparent material to facilitate real-time observation of the staining process. The slice incubation chamber 5, the liquid path 6 and the reagent well 7 can also be processed by integral injection molding.
[0033] The method for staining heart sections using the above device generally comprises the following steps:
[0034] (1) Before staining, mark the sample name in the recording area 9 of the sample area 8.
[0035] (2) According to the sample name, transfer the heart slices to the slice incubation chamber 5 of the corresponding sample area on the staining plate.
[0036] (3) After the heart slices are placed, place the upper staining plate 1 into the lower staining plate 2.
[0037] (4) Add TTC dye solution from the reagent well 7 so that the TTC dye solution fills the liquid path 6 and the slice incubation chamber 5, and the heart slices are immersed in the TTC dye solution.
[0038] (5) Place the upper staining plate 1 and the lower staining plate 2 together and incubate at 37 degrees for 30-45 minutes. During the staining process, remove the old TTC dye solution from the reagent well every 10 minutes and replenish with fresh TTC dye solution to ensure that the sections are in a good staining environment.
[0039] (6) After staining is completed, keep the staining plate 1 in place and remove the TTC staining solution from reagent well 7. Then add paraformaldehyde from reagent well 7 to immerse the heart slice.
[0040] (7) After fixation is completed, take out the heart slices and take photos or directly take photos of the stained plate, and finally analyze the area of the infarcted area using software.
[0041] In the above step (3), since the heart slices are placed on the upper staining plate 1, they will stick to the upper staining plate 1. Therefore, the upper staining plate 1 with the heart slices can be directly turned upside down on the lower staining plate 2. Of course, the lower staining plate 2 can also be placed on top of the upper staining plate 1 first, and then turned upside down.
[0042] The advantages of the present invention in the process of staining heart slices are specifically reflected in the following aspects:
[0043] 1. The utility model divides the staining plate into four sample areas through the arrangement of the liquid path, and each sample area is provided with a recording area. The sample name is pre-marked in the recording area before staining. This operation helps to avoid confusion when staining multiple heart slices at the same time.
[0044] 2. After the heart slices are cut, temporarily place them on ice bricks. According to the sample name, transfer the slices to a specific area on the staining plate. Each sample area can hold 6 slices. Specific slices can be selected and placed in the slice incubation chamber according to needs, or the slices can be placed in the slice incubation chamber one by one in the order from the apex to the auricle. The utility model can meet the needs of staining slices of 4 hearts at the same time, and the staining area of each heart is relatively independent. At the same time, the slice incubation chamber for each slice is also relatively independent. The design of the independent slice incubation chamber can both avoid the sticking of multiple slices and reduce the damage caused by sticking. The slice incubation chamber has a certain depth, and the depth is adapted to the thickness of the slice, which is the first key point to ensure the flatness of the slice. In addition, the simultaneous staining of multiple hearts can save a lot of time.
[0045] 3. After the sections are placed, place the upper staining tray into the lower staining tray. The design of the upper and lower staining trays creates a specific height for the sections to be stained. This is the second key to ensuring the flatness of the sections. It ensures that the sections are fully exposed to the dye solution while maintaining their original flatness.
[0046] 4. Add TTC dye from the reagent well, allowing the dye to fill the liquid path and the slice incubation chamber, immersing the slices in the dye. This differs from the traditional procedure of adding dye first and then covering the lid, or from simply placing the heart slices into a container pre-filled with dye. This invention stains while maintaining the relative position of the slices, which is the third key to ensuring smooth slices.
[0047] 5. The staining device is placed at 37 degrees for incubation, and fresh dye is supplied at any time through the reagent wells and liquid lines to ensure that the slices are in a good staining environment. This can avoid the operation of flipping the slices in traditional staining and avoid tissue damage caused by flipping.
[0048] 6. After staining is complete, maintain the upper staining plate in place and remove the TTC dye from the reagent wells. Then, add paraformaldehyde from the wells until the sections are fully submerged. Maintaining the upper staining plate while removing the TTC dye and adding paraformaldehyde is crucial for ensuring smooth sections. This process ensures that the sections remain confined to their designated space, preventing wrinkling and bulging. It also prevents the need to remove sections individually from the dye, minimizing tissue damage, ensuring section integrity, and saving significant processing time.
[0049] 7. After fixation is completed, remove the slices and take photos or directly take photos of the stained plate, and finally analyze the area of the infarcted area using software.
[0050] The utility model can reduce the difficulty of operating the heart slice staining, ensure the original appearance of the slice, improve the flatness and presentation of the slice, and improve the accuracy of the experimental results.
[0051] Parts not described in the above methods can be achieved by adopting or drawing on existing technologies.
[0052] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any improvements, equivalent replacements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.
Claims
1. A cardiac section staining device, characterized in that: The dyeing upper plate and the dyeing lower plate are included, wherein the dyeing upper plate is placed in the dyeing lower plate, and the bottom side of the dyeing upper plate is sealed and fitted with the top side of the bottom of the dyeing lower plate; A slice incubation chamber and a liquid path are provided at the bottom of the staining upper plate, the slice incubation chamber and the liquid path are connected, and a reagent hole is provided at the end of the liquid path; The slice incubation chamber and the liquid path are both formed by the bottom side of the staining upper plate protruding upward, the longitudinal cross section is groove-shaped, and is open at the bottom side of the staining upper plate; When the upper staining dish is placed in the lower staining dish, the openings of the slice incubation chamber and the liquid path are blocked by the top side of the bottom of the lower staining dish.
2. A cardiac section staining device according to claim 1, characterized in that: Each liquid path is connected to a plurality of slice incubation chambers.
3. The cardiac section staining device according to claim 1, characterized in that: There are two liquid circuits, namely the first liquid circuit and the second liquid circuit. The first liquid circuit and the second liquid circuit are arranged vertically and cross-wise to separate four sample areas. The first liquid circuit and the second liquid circuit are respectively connected to three slice incubation chambers corresponding to each sample area, and all slice incubation chambers are arranged along the length direction of the first liquid circuit or the second liquid circuit.
4. The cardiac section staining device according to claim 3, characterized in that: A recording area is provided in each sample area.
5. The cardiac section staining device according to claim 1, characterized in that: The reagent wells are arranged on the side of the staining upper plate.
6. The cardiac section staining device according to claim 1, characterized in that: The transverse cross section of the slice incubation chamber is arc-shaped, and the slice incubation chamber is connected with the liquid path at the arc-shaped notch.
7. The cardiac section staining device according to claim 1, characterized in that: The upper dyeing plate and the lower dyeing plate are both disc-shaped, the top of the lower dyeing plate is open and the inside is hollow; the inner diameter of the lower dyeing plate is 1.2-1.5 times the outer diameter of the upper dyeing plate, and the height of the upper dyeing plate is higher than that of the lower dyeing plate.
8. The cardiac section staining device according to claim 1, characterized in that: The dyeing upper plate is made of transparent material.
9. The cardiac section staining device according to claim 1, characterized in that: The height of the slice incubation chamber is 1.01-1.05 times the thickness of the heart slices.