Stem cell low-temperature preservation device

Abstract

The utility model discloses a stem cell cryopreservation device relates to stem cell cryopreservation technical field, including liquid nitrogen storage shell, liquid nitrogen storage shell top is fixedly connected with top plate through bolt, the inner wall of top plate is fixedly connected with placing cylinder, the inner wall of placing cylinder is symmetrically provided with a plurality of square through-hole, and the square through-hole is equipped with the through-hole. A rotating ring is rotatably connected to the top end of the placement barrel, a blocking ring is fixedly connected to the inner side, corresponding to the placement barrel, of the bottom end of the rotating ring, and a plurality of holes are symmetrically formed in the blocking ring in a penetrating mode. Liquid nitrogen in the liquid nitrogen storage shell is prevented from being volatilized into the external environment, reduction of the liquid nitrogen is avoided, so that the effect of reducing the liquid nitrogen adding frequency is achieved, and the problem that a traditional low-temperature preservation device is in an open state when stem cell test tubes are taken and placed, and consequently the liquid nitrogen in the device is rapidly reduced is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell cryopreservation, in particular to a stem cell cryopreservation device. Background Art

[0002] Stem cells are a type of multipotent cell with the ability to self-renew. Under certain conditions, they can differentiate into a variety of multipotent cells. They are a type of primitive cells with the potential for self-replication and multidirectional differentiation. That is, stem cells remain in an undifferentiated state and have the ability to proliferate. Under appropriate conditions or given appropriate signals, they can differentiate into a variety of functional cells or tissues and organs. According to the developmental stage of stem cells, they are divided into embryonic stem cells and adult stem cells. In order to maintain the activity of the stem cells themselves, they need to be stored in a low-temperature environment when they are preserved, so they need to be stored in a low-temperature preservation device.

[0003] Currently, existing cryogenic storage devices require the cover to be fully opened during the access process. When open, the liquid nitrogen inside will evaporate into the external environment, resulting in a decrease in liquid nitrogen, necessitating frequent replenishment of liquid nitrogen.

[0004] To this end, this case proposes a stem cell cryopreservation device to solve the above technical problems. Utility Model Content

[0005] The main purpose of the present invention is to provide a stem cell cryopreservation device that can effectively solve the technical problems in the background technology.

[0006] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0007] A stem cell cryopreservation device comprises a liquid nitrogen storage shell, the top of the liquid nitrogen storage shell is fixedly connected to a top plate by bolts, the inner wall of the top plate is fixedly connected to a placement tube, the inner wall of the placement tube is symmetrically provided with a plurality of square through holes, the top of the placement tube is rotatably connected to a rotating ring, the bottom end of the rotating ring is fixedly connected to a blocking ring corresponding to the inner side of the placement tube, the blocking ring is symmetrically provided with a plurality of holes, the outer wall of the rotating ring is provided with two bayonet holes, the top of the top plate is fixedly connected to a fixed block on one side corresponding to the rotating ring, the end of the fixed block close to the rotating ring is fixedly connected to a first spring, the other end of the first spring is fixedly connected to a moving block, the other end of the moving block is fixedly connected to a card plate adapted to the shape of the bayonet, and the moving block and the top plate are slidably connected.

[0008] As a further solution of the present invention, a cover plate is provided at the top of the rotating ring, and a plurality of support rods are fixedly connected to the inner side of the rotating ring at the bottom end of the cover plate, and a plurality of support rods are fixedly connected to the bottom ends of the support rods. A plurality of grooves are provided between the top end of the base plate and the corresponding support rods.

[0009] As a further solution of the present invention, a limit plate is fixedly connected to the outer wall of the support rod corresponding to the upper side of the bottom plate, and the limit plate is provided with a plurality of limit holes for placing stem cell test tubes.

[0010] As a further solution of the present invention, multiple outer walls of the support rods are slidably connected to the upper side of the limit plates corresponding to the extrusion plates, and multiple second springs are fixedly connected to the top of the extrusion plates, and the multiple second springs are fixedly connected to the cover plate.

[0011] As a further solution of the present invention, a plurality of positioning grooves are provided at the bottom end of the extrusion plate, and the plurality of positioning grooves are adapted to the shape of the top end of the stem cell test tube.

[0012] As a further solution of the present invention, a fixing ring is fixedly connected to the outer side of the support rod at the bottom end of the cover plate, and an annular groove matching the shape of the fixing ring is provided at the top end of the rotating ring.

[0013] As a further solution of the present invention, a distance is provided between the bottom of the inner wall of the liquid nitrogen storage shell and the bottom end of the placement cylinder, and liquid nitrogen is placed at the bottom of the inner wall of the liquid nitrogen storage shell.

[0014] The beneficial effects of the utility model are as follows:

[0015] The square through-hole can be blocked by the cooperation of the movable block, the clamping plate, the bayonet, the rotating ring, the first spring and the blocking ring. Therefore, when the stem cell test tube is taken out, the liquid nitrogen in the liquid nitrogen storage shell is prevented from volatilizing into the external environment, thereby avoiding the rapid reduction of liquid nitrogen and reducing the number of times liquid nitrogen is added.

[0016] Through the mutual cooperation of the bottom plate, the limiting plate, the second spring, the extrusion plate, the positioning slot and the groove, it is possible to maintain stability when taking and placing the stem cell test tube, prevent shaking and breakage, and thus improve safety. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the overall structure of a stem cell cryopreservation device of the present invention;

[0018] Figure 2 for Figure 1 A magnified view of middle A;

[0019] Figure 3 This is a vertical cross-sectional view of a liquid nitrogen storage shell of a stem cell cryopreservation device of the present invention;

[0020] Figure 4 This is a vertical cross-sectional view of a storage tube of a stem cell cryopreservation device according to the present invention;

[0021] Figure 5This is a disassembled diagram of a stem cell cryopreservation device according to the present invention;

[0022] Figure 6 This is an exploded bottom view of a stem cell cryopreservation device according to the present invention.

[0023] In the figure: 1. Liquid nitrogen storage shell; 2. Top plate; 3. Placement cylinder; 4. Square through hole; 5. Rotating ring; 6. Blocking ring; 7. Bayonet; 8. Fixed block; 9. First spring; 10. Moving block; 11. Card plate; 12. Cover plate; 13. Support rod; 14. Bottom plate; 15. Limit plate; 16. Second spring; 17. Extrusion plate; 18. Positioning groove; 19. Fixed ring; 20. Annular groove. DETAILED DESCRIPTION

[0024] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific implementation methods.

[0025] like Figure 1-6 As shown, a stem cell cryopreservation device includes a liquid nitrogen storage shell 1, the top of the liquid nitrogen storage shell 1 is fixedly connected to a top plate 2 by bolts, the inner wall of the top plate 2 is fixedly connected to a placement tube 3, the inner wall of the placement tube 3 is symmetrically perforated with a plurality of square through holes 4, the top of the placement tube 3 is rotatably connected to a rotating ring 5, the bottom end of the rotating ring 5 is fixedly connected to a blocking ring 6 corresponding to the inner side of the placement tube 3, the blocking ring 6 is symmetrically perforated with a plurality of holes, the outer wall of the rotating ring 5 is provided with two bayonet holes 7, the top of the top plate 2 is fixedly connected to a fixed block 8 corresponding to one side of the rotating ring 5, the end of the fixed block 8 close to the rotating ring 5 is fixedly connected to a first spring 9, the other end of the first spring 9 is fixedly connected to a moving block 10, the other end of the moving block 10 is fixedly connected to a card plate 11 adapted to the shape of the bayonet hole 7, and the moving block 10 is slidably connected to the top plate 2;

[0026] When a stem cell test tube needs to be placed, the limit on the rotating ring 5 is released, and then the rotating ring 5 is rotated in the opposite direction to open the square through hole 4. At this time, the liquid nitrogen in the liquid nitrogen storage shell 1 performs a low-temperature preservation treatment on the stem cells in the stem cell test tube.

[0027] In this embodiment, a cover plate 12 is provided at the top of the rotating ring 5. A plurality of support rods 13 are fixedly connected to the bottom end of the cover plate 12 corresponding to the inner side of the rotating ring 5. The bottom ends of the plurality of support rods 13 are fixedly connected to a bottom plate 14. A plurality of grooves are provided between the top ends of the bottom plate 14 and the corresponding support rods 13.

[0028] The cover plate 12 and the floor plate 14 are connected together by the support rods 13 to form a whole, and the groove is adapted to the shape of the bottom of the stem cell test tube for placing the stem cell test tube.

[0029] In this embodiment, a limiting plate 15 is fixedly connected to the outer wall of the support rod 13 above the bottom plate 14. The limiting plate 15 is provided with a plurality of limiting holes for placing stem cell test tubes, and the limiting plate 15 can limit the placement of the stem cell test tubes.

[0030] In this embodiment, an extrusion plate 17 is slidably connected to the outer wall of the plurality of support rods 13 above the corresponding limit plate 15. A plurality of second springs 16 are fixedly connected to the top of the extrusion plate 17. The plurality of second springs 16 are fixedly connected to the cover plate 12. A plurality of positioning grooves 18 are formed at the bottom end of the extrusion plate 17. The plurality of positioning grooves 18 are adapted to the shape of the top of the stem cell test tube.

[0031] The second spring 16 can move the extrusion plate 17 downward, so that the positioning groove 18 is stuck on the top of the stem cell test tube, thereby preventing shaking when taking and placing the stem cell test tube, keeping it stable and improving safety.

[0032] In this embodiment, a fixing ring 19 is fixedly connected to the outer side of the support rod 13 at the bottom end of the cover plate 12, and an annular groove 20 that matches the shape of the fixing ring 19 is provided at the top end of the rotating ring 5. The friction between the fixing ring 19 and the annular groove 20 can enable the cover plate 12 to be installed on the top end of the rotating ring 5.

[0033] In this embodiment, a distance is set between the bottom of the inner wall of the liquid nitrogen storage shell 1 and the bottom end of the placement cylinder 3. Liquid nitrogen is placed at the bottom of the inner wall of the liquid nitrogen storage shell 1, and the liquid level of the liquid nitrogen is lower than that of the placement cylinder 3.

[0034] It should be noted that the present invention is a stem cell cryopreservation device. When in use, the movable block 10 can be pushed outward to allow the clamping plate 11 to leave the range of the clamping port 7. At this time, the rotating ring 5 is rotated until the clamping plate 11 corresponds to the position of the other clamping port 7. The first spring 9 elastically acts to insert the clamping plate 11 into the other clamping port 7. At this time, the square through hole 4 is blocked by the blocking ring 6. Therefore, when the stem cell test tube is removed, the liquid nitrogen in the liquid nitrogen storage shell 1 is prevented from volatilizing into the external environment, thereby avoiding the reduction of liquid nitrogen and reducing the number of times liquid nitrogen is added.

[0035] When the stem cell test tube is placed on the bottom plate 14 and the limiting plate 15, the elasticity of the second spring 16 pushes the extrusion plate 17 downward, so that the positioning groove 18 is stuck on the top of the stem cell test tube, and the bottom end of the stem cell test tube is stuck in the groove. Therefore, the stem cell test tube can be kept stable when being taken and placed, and prevented from shaking and breaking.

[0036] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and improvements may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and improvements are intended to fall within the scope of the present invention. The scope of protection claimed in this invention is defined by the appended claims and their equivalents.

Claims

1. A stem cell cryopreservation device comprising a liquid nitrogen storage shell (1), characterized in that: The top of the liquid nitrogen storage shell (1) is fixedly connected to a top plate (2) by bolts, and the inner wall of the top plate (2) is fixedly connected to a placement tube (3), and the inner wall of the placement tube (3) is symmetrically provided with a plurality of square through holes (4), and the top of the placement tube (3) is rotatably connected to a rotating ring (5), and the bottom end of the rotating ring (5) is fixedly connected to a blocking ring (6) corresponding to the inner side of the placement tube (3), and the blocking ring (6) is symmetrically provided with a plurality of holes, and the outer wall of the rotating ring (5) is provided with two bayonet holes (7), and the top of the top plate (2) is fixedly connected to a fixed block (8) on one side corresponding to the rotating ring (5), and the fixed block (8) is fixedly connected to a first spring (9) at one end close to the rotating ring (5), and the other end of the first spring (9) is fixedly connected to a moving block (10), and the other end of the moving block (10) is fixedly connected to a card plate (11) that matches the shape of the bayonet hole (7), and the moving block (10) and the top plate (2) are slidably connected.

2. The stem cell cryopreservation device according to claim 1, characterized in that: A cover plate (12) is provided at the top of the rotating ring (5); a plurality of support rods (13) are fixedly connected to the bottom of the cover plate (12) corresponding to the inner side of the rotating ring (5); a bottom plate (14) is fixedly connected to the bottom ends of the plurality of support rods (13); and a plurality of grooves are provided between the top ends of the bottom plate (14) and the corresponding support rods (13).

3. The stem cell cryopreservation device according to claim 2, characterized in that: A limiting plate (15) is fixedly connected to the upper portion of the outer wall of the support rod (13) corresponding to the bottom plate (14), and the limiting plate (15) is provided with a plurality of limiting holes for placing stem cell test tubes.

4. The stem cell cryopreservation device according to claim 2, characterized in that: An extrusion plate (17) is slidably connected to the upper side of the corresponding limit plate (15) on the outer wall of the plurality of support rods (13); a plurality of second springs (16) are fixedly connected to the top of the extrusion plate (17); and the plurality of second springs (16) are all fixedly connected to the cover plate (12).

5. The stem cell cryopreservation device according to claim 4, characterized in that: The bottom end of the squeezing plate (17) is provided with a plurality of positioning grooves (18), and the plurality of positioning grooves (18) are adapted to the shape of the top end of the stem cell test tube.

6. The stem cell cryopreservation device according to claim 2, characterized in that: A fixing ring (19) is fixedly connected to the outer side of the supporting rod (13) at the bottom end of the cover plate (12), and an annular groove (20) matching the shape of the fixing ring (19) is formed at the top end of the rotating ring (5).

7. The stem cell cryopreservation device according to claim 1, characterized in that: A distance is provided between the bottom of the inner wall of the liquid nitrogen storage shell (1) and the bottom end of the placement cylinder (3), and liquid nitrogen is placed at the bottom of the inner wall of the liquid nitrogen storage shell (1).

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