Stem cell culture proliferation device

Abstract

The utility model discloses a stem cell culture proliferation device which comprises a culture box, a box door is hinged to the culture box, a rotating shaft is vertically and rotatably connected to the interior of the culture box, a plurality of placing trays are slidably connected to the rotating shaft at intervals in a sleeved mode, and a plurality of placing grooves which are distributed along the circumference and used for placing culture flasks for stem cell culture are formed in the top faces of the placing trays at intervals. The two sides of each placing groove are fixedly connected with clamping assemblies respectively, and the clamping assemblies are used for clamping and fixing the tops of the culture bottles; a limiting assembly is fixedly connected to the bottom of the containing disc and used for fixing the containing disc to the rotating shaft. By clamping and fixing the culture bottles, the culture bottles can be stabilized on the placing plates, the culture bottles are prevented from turning over due to accidental touch or shaking, meanwhile, the distance between every two adjacent placing plates can be changed, and the applicability of the device is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell culture equipment, in particular to a stem cell culture and proliferation device. Background Art

[0002] Stem cells are a type of cell with unlimited or immortal self-renewal capacity, capable of producing at least one type of highly differentiated progeny cells. Stem cell proliferation and culture technology is the foundation and prerequisite for stem cell research. Through in vitro culture, stem cells can be expanded in large quantities, providing an adequate cell source for subsequent cell therapy, tissue engineering, and other applications. They have broad clinical application prospects in areas such as cell therapy, system reconstruction, tissue engineering, gene therapy, and cosmetic and anti-aging. When culturing stem cells experimentally, a cell culture proliferation device is typically used to cultivate the culture medium in a culture dish or flask.

[0003] Existing stem cell culture and proliferation devices typically lack dedicated fixing mechanisms to secure culture bottles or dishes. For example, utility model patent application number 202122466307.9 discloses a stem cell culture and proliferation device. This utility model utilizes an adjustment mechanism to allow workers to easily adjust the position of the support plate based on the actual height of the culture bottle when placing the culture bottle into the incubator. This adjustment mechanism uses a slot to adjust the position of the support plate to accommodate the actual height of the culture bottle, greatly expanding the incubator's usability.

[0004] However, when using this device, to retrieve a culture bottle, one must manually rotate the placement plate to position the desired bottle to a convenient location before removing it. Because there is no mechanism to secure the bottle, the placement plate can easily cause the bottle to shake and tip over during rotation. Furthermore, when removing a bottle, it's easy to accidentally bump into another bottle, causing it to tip over, potentially damaging the bottle or leaking the stem cells inside.

[0005] Therefore, it is necessary to develop a stem cell culture and proliferation device to address the above-mentioned defects. Utility Model Content

[0006] The purpose of the utility model is to provide a stem cell culture and proliferation device, which can stabilize the culture bottle on the placement plate by clamping and fixing the culture bottle, avoid the culture bottle from tipping over due to accidental collision or shaking, and at the same time can change the distance between two adjacent placement plates to improve the applicability of the device.

[0007] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0008] The utility model discloses a stem cell culture and proliferation device, comprising an incubator, wherein the incubator is hingedly provided with a door, a rotating shaft is vertically rotatably connected inside the incubator, a plurality of placement trays are slidably sleeved on the rotating shaft at intervals, a plurality of placement slots for placing culture bottles for stem cell culture are arranged at intervals on the top surface of the placement tray, distributed along the circumference; a clamping assembly is fixedly connected on both sides of each of the placement slots, the clamping assembly is used to clamp and fix the top of the culture bottle; a limiting assembly is fixedly connected to the bottom of the placement tray, and the limiting assembly is used to fix the placement tray on the rotating shaft.

[0009] Furthermore, the clamping assembly includes a splint, a connecting rod, a first baffle, a first compression spring and a connecting tube; one end of the first compression spring is fixedly connected to the inner bottom surface of the connecting tube, the first baffle is slidably connected in the connecting tube, one end of the connecting rod is fixedly connected to the end surface of the first baffle, and the other end extends out of the connecting tube and is slidably connected to the through hole opened on the connecting tube, the splint is fixedly connected to the end of the connecting rod extending out of the connecting tube; the connecting tube is fixedly connected to an adjustment assembly at one end away from the splint, and the adjustment assembly is used to support and adjust the height of the clamping assembly.

[0010] Furthermore, the adjusting assembly includes a bracket, a threaded barrel, an adjusting rod, a threaded block, a connecting plate and a first limit plate; the threaded barrel is vertically rotatably connected to the top surface of the placing disk, the threaded block is threadedly connected in the threaded barrel, the adjusting rod is fixedly connected to the threaded block, the connecting plate is fixedly connected to the top surface of the adjusting rod, and the connecting barrel is fixedly connected to the side surface of the connecting plate; one end of the bracket is fixedly connected to the top surface of the placing disk, and the other end is fixedly connected to the first limit plate, the adjusting rod passes through the first limit plate and is slidably connected to it; a limit strip is vertically fixedly connected to the outer wall of the adjusting rod, and a limit slot is correspondingly provided on the first limit plate, and the limit strip is slidably connected to the limit slot.

[0011] Furthermore, the limiting assembly includes a pull rod, a second compression spring, an insert rod, a second baffle and a fixed block; the fixed block is fixedly connected to the bottom surface of the placement plate, the pull rod passes through the fixed block and is slidably connected thereto, and the pull rod is fixedly connected to one end of the second baffle near the rotating shaft; the second compression spring is sleeved on the pull rod, and one end of the second compression spring is fixedly connected to the fixed block; a number of limiting holes are provided at intervals on the rotating shaft, and the insert rod is fixedly connected to the second baffle and is slidably connected to the limiting holes.

[0012] Furthermore, there are two groups of the limiting components, which are arranged on both sides of the rotating shaft.

[0013] Furthermore, the pull rod is fixedly connected to a second limiting plate at one end away from the rotating shaft, and a groove is provided on the placement plate, and the groove is used to limit the second limiting plate.

[0014] Furthermore, a servo motor is fixedly connected to the top surface of the incubator, and a rotating shaft at an output end of the servo motor is coaxially fixedly connected to the rotating shaft.

[0015] Compared with the prior art, the beneficial technical effects of the present invention are:

[0016] The present invention, through the arrangement of the clamping assembly and the placement slot, can clamp and secure the culture bottles, stabilizing them on the placement tray and preventing them from tipping over due to accidental contact or shaking. Furthermore, by allowing the placement tray to move up and down on the rotating shaft, the spacing between two adjacent placement trays can be adjusted according to the size and height of the culture bottles, thereby improving the applicability of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The present invention will be further described below with reference to the accompanying drawings.

[0018] Figure 1 This is a schematic diagram of the three-dimensional structure of the stem cell culture and proliferation device of the present invention;

[0019] Figure 2 This is a top-down perspective structural diagram of the placement tray of the present invention;

[0020] Figure 3 This is a bottom-up perspective structural diagram of the placement tray of the present invention;

[0021] Figure 4 This is a schematic diagram of the three-dimensional structure of the clamping assembly and the adjusting assembly of the present invention after being decomposed.

[0022] Explanation of the accompanying drawings: 1. incubator; 2. door; 3. rotating shaft; 4. placement plate; 5. placement slot; 6. culture bottle; 7. clamping assembly; 701. clamping plate; 702. connecting rod; 703. first baffle; 704. first compression spring; 705. connecting cylinder; 8. limiting assembly; 801. pull rod; 802. second compression spring; 803. insert rod; 804. second baffle; 805. fixing block; 806. limiting hole; 807. second limiting plate; 808. groove; 9. adjusting assembly; 901. bracket; 902. threaded cylinder; 903. adjusting rod; 904. threaded block; 905. connecting plate; 906. first limiting plate; 907. limiting strip; 908. limiting slot; 10. servo motor. DETAILED DESCRIPTION

[0023] The core of the utility model is to provide a stem cell culture and proliferation device, which can stabilize the culture bottle on the placement tray by clamping and fixing the culture bottle, preventing the culture bottle from tipping over due to accidental collision or shaking, and at the same time can change the distance between two adjacent placement trays to improve the applicability of the device.

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0025] In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they cannot be understood as limitations on the present invention.

[0026] In one embodiment, Figure 1 and Figure 2 As shown, it includes an incubator 1, which is hinged with a door 2. A rotating shaft 3 is vertically connected inside the incubator 1, and a number of placement trays 4 are slidably sleeved on the rotating shaft 3 at intervals. The top surface of the placement tray 4 is provided with a number of placement grooves 5 distributed along the circumference for placing culture bottles 6 for stem cell culture; each of the placement grooves 5 is fixedly connected to a clamping assembly 7 on both sides, and the clamping assembly 7 is used to clamp and fix the top of the culture bottle 6; the bottom of the placement tray 4 is fixedly connected to a limiting assembly 8, and the limiting assembly 8 is used to fix the placement tray 4 on the rotating shaft 3.

[0027] The clamping assembly 7 and placement slot 5 are configured to clamp and secure a culture bottle 6 placed in the placement slot 5, stabilizing the culture bottle 6 on the placement tray 4 and preventing the culture bottle 6 from tipping over due to accidental contact or shaking. Furthermore, by allowing the placement tray 4 to move up and down on the rotating shaft 3, the spacing between two adjacent placement trays 4 can be adjusted according to the size and height of the culture bottle 6, thereby improving the device's applicability.

[0028] In one embodiment, Figure 1 、 Figure 2 and Figure 4As shown, the clamping assembly 7 includes a clamping plate 701, a connecting rod 702, a first baffle 703, a first compression spring 704 and a connecting tube 705; one end of the first compression spring 704 is fixedly connected to the inner bottom surface of the connecting tube 705, the first baffle 703 is slidably connected in the connecting tube 705, one end of the connecting rod 702 is fixedly connected to the end surface of the first baffle 703, and the other end extends out of the connecting tube 705 and is slidably connected to the through hole opened on the connecting tube 705, the clamping plate 701 is fixedly connected to the end of the connecting rod 702 extending out of the connecting tube 705; the connecting tube 705 is fixedly connected to an adjustment assembly 9 away from the clamping plate 701, and the adjustment assembly 9 is used to support and adjust the height of the clamping assembly 7.

[0029] It should be noted that in the initial state where no culture bottle 6 is clamped between the two clamping assemblies 7 , the first compression spring 704 in the connecting tube 705 is in a compressed state, and the first baffles 703 of the two clamping assemblies 7 are in contact with each other.

[0030] Through the cooperation of the clamping plates 701, the connecting rod 702, the first baffle 703, and the first compression spring 704, when the two clamping assemblies 7 clamp and secure the culture bottle 6, the first baffle 703 of each clamping assembly 7 slides within the connecting tube 705 toward one end of the culture bottle 6 under the restoring force of the first compression spring 704, driving the connecting rod 702 and the clamping plates 701 to move toward the culture bottle 6, thereby simultaneously approaching the culture bottle 6 and clamping and securing the culture bottle 6.

[0031] Specifically, the adjusting assembly 9 includes a bracket 901, a threaded barrel 902, an adjusting rod 903, a threaded block 904, a connecting plate 905 and a first limit plate 906; the threaded barrel 902 is vertically rotatably connected to the top surface of the placement disk 4, the threaded block 904 is threadedly connected to the threaded barrel 902, the adjusting rod 903 is fixedly connected to the threaded block 904, the connecting plate 905 is fixedly connected to the top surface of the adjusting rod 903, and the connecting barrel 705 is fixedly connected to the side surface of the connecting plate 905; one end of the bracket 901 is fixedly connected to the top surface of the placement disk 4, and the other end is fixedly connected to the first limit plate 906, the adjusting rod 903 passes through the first limit plate 906 and is slidably connected thereto; a limit strip 907 is vertically fixedly connected to the outer wall of the adjusting rod 903, and a limit groove 908 is correspondingly provided on the first limit plate 906, and the limit strip 907 is slidably connected to the limit groove 908.

[0032] This arrangement supports and adjusts the height of the clamping assembly 7. The first limiting plate 906, limiting bar 907, and limiting groove 908 provide a sliding guide for the adjustment rod 903 while preventing it from rotating. By manually rotating the threaded barrel 902, the threaded barrel 902 drives the threaded block 904, which is threadedly connected thereto, to move up and down within the barrel 902. This further drives the adjustment rod 903, which is fixedly connected to the threaded block 904, to move synchronously, thereby driving the clamping assembly 7, which is fixedly connected to the adjustment rod 903, to move up and down, achieving the purpose of adjusting the height of the clamping plate 701. This makes it suitable for clamping and fixing culture bottles 6 of different heights, further improving the applicability of the device.

[0033] In one embodiment, Figure 3 As shown, the limiting assembly 8 includes a pull rod 801, a second compression spring 802, an insert rod 803, a second baffle 804 and a fixed block 805; the fixed block 805 is fixedly connected to the bottom surface of the placement plate 4, the pull rod 801 passes through the fixed block 805 and is slidably connected thereto, and the end of the pull rod 801 close to the rotating shaft 3 is fixedly connected to the end of the second baffle 804; the second compression spring 802 is sleeved on the pull rod 801, and one end of the second compression spring 802 is fixedly connected to the fixed block 805; a number of limiting holes 806 are provided at intervals through the rotating shaft 3, and the insert rod 803 is fixedly connected to the second baffle 804 and is slidably connected to the limiting holes 806.

[0034] By inserting the insertion rod 803 into the limiting hole 806 of the rotating shaft 3, the placement tray 4 can be fixed on the rotating shaft 3, preventing the placement tray 4 from sliding up and down on the rotating shaft 3, and also preventing the placement tray 4 from rotating on the rotating shaft 3. At the same time, the action of the second compression spring 802 can prevent the insertion rod 803 from sliding out of the limiting hole 806, thereby improving the stability of the device.

[0035] Specifically, there are two groups of limiting components 8 , which are arranged on both sides of the rotating shaft 3 .

[0036] By such an arrangement, the fixing effect of the limiting component 8 on the placement tray 4 can be improved.

[0037] Specifically, one end of the pull rod 801 away from the rotating shaft 3 is fixedly connected to the second limiting plate 807 , and a groove 808 is opened on the placement plate 4 , and the groove 808 is used to limit the second limiting plate 807 .

[0038] It should be noted that when the second stop plate 807 on the pull rod 801 is pulled to the groove 808, it is necessary to ensure that the insertion rod 803 is completely out of the stop hole 806 to avoid affecting the sliding of the placement tray 4 on the rotating shaft 3. One end of the second stop plate 807 is fixedly connected to the pull rod 801, and the distance between the other end and the outer wall of the pull rod 801 is greater than the distance between the pull rod 801 and the placement tray 4.

[0039] By pulling the end of the pull rod 801 away from the rotating shaft 3, the insertion rod 803 is driven away from the rotating shaft 3 until it is completely out of the limiting hole 806. At this time, the pull rod 801 is rotated so that the end of the second limiting plate 807 not fixedly connected to the pull rod 801 is inserted into the groove 808. This facilitates the up and down movement of the placement trays 4 on the rotating shaft 3 when adjusting the distance between the two placement trays 4.

[0040] In one embodiment, Figure 1 As shown, a servo motor 10 is fixedly connected to the top surface of the incubator 1 , and the output end shaft of the servo motor 10 is coaxially fixedly connected to the shaft 3 .

[0041] By setting the servo motor 10, the rotation of the servo motor 10 can be controlled to drive the rotating shaft 3 to rotate, so that when placing and removing the culture bottle 6, the corresponding position of the placement tray 4 is rotated to the box door 2, which facilitates the placement and removal of the culture bottle 6.

[0042] Working principle of the present invention: When using the stem cell culture and proliferation device of the present invention, first adjust the distance between two adjacent placement trays 4 according to the height of the culture bottle 6, manually move the placement tray 4, slide the placement tray 4 on the rotating shaft 3 to a suitable position, and then insert the insertion rod 803 into the limiting hole 806 to fix the position of the placement tray 4.

[0043] Then adjust the height of the splint 701 by manually rotating the threaded barrel 902. The threaded barrel 902 drives the threaded block 904 threadedly connected to it to move up and down in the threaded barrel 902, and further drives the adjusting rod 903 fixedly connected to the threaded block 904 to move synchronously, thereby driving the clamping assembly 7 fixedly connected to the adjusting rod 903 to move up and down, thereby achieving the purpose of adjusting the height of the splint 701.

[0044] Finally, the two clamps 701 are manually separated so that they are away from each other. Then, the culture bottle 6 containing the stem cell culture medium and stem cells is placed on the placement groove 5 of the placement tray 4. The clamps 701 are loosened. The clamps 701 move closer to each other under the action of the restoring force of the first compression spring 704, thereby contacting the top of the culture bottle 6 to clamp it firmly.

[0045] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. Reference can be made to the common and similar parts between the various embodiments. For the devices disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the description is relatively simple, and the relevant parts can be referred to the method description.

[0046] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements to the technical solutions of the present invention made by ordinary technicians in this field should fall within the scope of protection determined by the claims of the present invention.

Claims

1. A stem cell culture and proliferation device, comprising an incubator (1), wherein the incubator (1) is hingedly provided with a door (2), and wherein: The incubator (1) is vertically rotatably connected to a rotating shaft (3), and a plurality of placement trays (4) are slidably sleeved on the rotating shaft (3) at intervals. The top surface of the placement tray (4) is provided with a plurality of placement grooves (5) distributed along the circumference for placing culture bottles (6) for stem cell culture; each of the placement grooves (5) is fixedly connected to two sides with a clamping assembly (7), and the clamping assembly (7) is used to clamp and fix the top of the culture bottle (6); the bottom of the placement tray (4) is fixedly connected to a limiting assembly (8), and the limiting assembly (8) is used to fix the placement tray (4) on the rotating shaft (3).

2. The stem cell culture and proliferation device according to claim 1, characterized in that: The clamping assembly (7) includes a clamping plate (701), a connecting rod (702), a first baffle (703), a first compression spring (704) and a connecting tube (705); one end of the first compression spring (704) is fixedly connected to the inner bottom surface of the connecting tube (705), the first baffle (703) is slidably connected in the connecting tube (705), one end of the connecting rod (702) is fixedly connected to the end surface of the first baffle (703), and the other end extends out of the connecting tube (705) and is slidably connected to a through hole provided on the connecting tube (705), the clamping plate (701) is fixedly connected to the end of the connecting rod (702) extending out of the connecting tube (705); the end of the connecting tube (705) away from the clamping plate (701) is fixedly connected to an adjustment assembly (9), and the adjustment assembly (9) is used to support and adjust the height of the clamping assembly (7).

3. The stem cell culture and proliferation device according to claim 2, characterized in that: The adjusting assembly (9) comprises a bracket (901), a threaded barrel (902), an adjusting rod (903), a threaded block (904), a connecting plate (905) and a first limiting plate (906); the threaded barrel (902) is vertically rotatably connected to the top surface of the placement plate (4); the threaded block (904) is threadedly connected inside the threaded barrel (902); the adjusting rod (903) is fixedly connected to the threaded block (904); the connecting plate (905) is fixedly connected to the top surface of the adjusting rod (903); the connecting barrel (7 05) is fixedly connected to the side of the connecting plate (905); one end of the bracket (901) is fixedly connected to the top surface of the placement plate (4), and the other end is fixedly connected to the first limiting plate (906), and the adjusting rod (903) passes through the first limiting plate (906) and is slidably connected thereto; a limiting strip (907) is vertically fixedly connected to the outer wall of the adjusting rod (903), and a limiting groove (908) is correspondingly provided on the first limiting plate (906), and the limiting strip (907) is slidably connected to the limiting groove (908).

4. The stem cell culture and proliferation device according to claim 1, characterized in that: The limiting assembly (8) includes a pull rod (801), a second compression spring (802), an insertion rod (803), a second baffle (804) and a fixed block (805); the fixed block (805) is fixedly connected to the bottom surface of the placement plate (4), the pull rod (801) passes through the fixed block (805) and is slidably connected thereto, and the pull rod (801) is fixedly connected to one end of the second baffle (804) near the rotating shaft (3); the second compression spring (802) is sleeved on the pull rod (801), and one end of the second compression spring (802) is fixedly connected to the fixed block (805); a plurality of limiting holes (806) are provided on the rotating shaft (3) at intervals, and the insertion rod (803) is fixedly connected to the second baffle (804) and is slidably connected to the limiting holes (806).

5. The stem cell culture and proliferation device according to claim 4, characterized in that: The number of the limiting components (8) is two and they are arranged on both sides of the rotating shaft (3) facing each other.

6. The stem cell culture and proliferation device according to claim 5, characterized in that: The pull rod (801) is fixedly connected to a second limiting plate (807) at one end away from the rotating shaft (3), and a groove (808) is provided on the placement plate (4), and the groove (808) is used to limit the second limiting plate (807).

7. The stem cell culture and proliferation device according to claim 1, characterized in that: A servo motor (10) is fixedly connected to the top surface of the incubator (1), and the output end rotating shaft of the servo motor (10) is coaxially fixedly connected to the rotating shaft (3).

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