Plant exosome separation device

Abstract

The utility model relates to the technical field of exosome separation, and discloses a plant exosome separation device which comprises a base, a filter assembly is arranged on the right side of the top of the base and used for filtering and separating exosomes, a crushing tank is fixedly connected to the left side of the top of the base, a filter screen is arranged in the crushing tank, and the filter screen is fixedly connected to the right side of the top of the base. And a crushing assembly is arranged on the inner side, close to the filter screen, of the interior of the crushing tank, the crushing assembly is used for crushing plant fragments, the filter assembly comprises a first peristaltic pump, and the first peristaltic pump is fixedly connected to the top of the base. According to the plant exosome separation device disclosed by the utility model, after the crushed tissue fragments are crushed, an extracting solution can be subjected to multi-stage layer-by-layer filtration under the action of structures such as the filter drum I and the filter drum II, so that impurities contained in the extracting solution can be fully removed, the purity of separated exosomes is improved, and high-purity plant exosomes are provided for subsequent use; and the product quality is further improved.

Description

Technical Field

[0001] The utility model relates to the technical field of exosome separation, and in particular to a plant exosome separation device. Background Art

[0002] Plant exosomes are small vesicles secreted by plant cells, usually with a diameter between 50 and 200 nanometers, mainly composed of a lipid bilayer membrane. They transmit signals and substances between plant cells and participate in physiological processes such as regulating growth, development, and responding to environmental stress. Studies have shown that plant exosomes play an important role in plant disease resistance, coping with adversity, and interacting with microorganisms. Therefore, they have potential application value in agriculture and plant biotechnology, so it is necessary to isolate plant exosomes to facilitate research.

[0003] When extracting plant exosomes, the exosomes are generally separated from the plant tissue fluid by filtering it. First, the plant tissue fragments are crushed so that the plant tissue is fully broken and the exosomes can be released into the subsequent extract. Then the extract produced after the crushing is filtered to separate the plant exosomes from the extract to obtain the desired plant exosomes.

[0004] However, the existing filtration method is relatively simple, and it will perform a single filtration through a filter membrane with an extremely small pore size. Since the extract contains a large amount of impurities, the impurities will accumulate during filtration, which will slow down the filtration speed and reduce the separation efficiency. At the same time, the extract cannot be fully filtered, and it still contains certain impurities, resulting in the low purity of the separated exosomes, affecting subsequent use. Therefore, those skilled in the art have proposed a plant exosome separation device to solve the above problems. Utility Model Content

[0005] In order to make up for the above shortcomings, the utility model provides a plant exosome separation device, which aims to improve the problems of slow filtration efficiency and low purity of separated exosomes in the existing technology during plant exosome separation.

[0006] To achieve the above objectives, the present invention provides the following technical solutions: a plant exosome separation device, comprising a base, a filter assembly provided on the top right side of the base, the filter assembly being used to filter and separate exosomes, a crushing tank fixedly connected to the top left side of the base, a filter screen provided inside the crushing tank, a crushing assembly provided inside the crushing tank near the filter screen, the crushing assembly being used to crush plant fragments;

[0007] The filtering assembly includes a peristaltic pump 1, which is fixedly connected to the top of the base, the input end of the peristaltic pump 1 is connected to the bottom through hole of the crushing tank, the output end of the peristaltic pump 1 is connected to the filter cartridge 1 through a pipeline, the bottom of the filter cartridge 1 is connected to the peristaltic pump 2 through a pipeline, the output end of the peristaltic pump 2 is connected to the filter cartridge 2 through a pipeline, the bottom of the filter cartridge 2 is connected to the peristaltic pump 3 through a pipeline, the output end of the peristaltic pump 3 is connected to the filter cartridge 3 through a pipeline, the bottom of the filter cartridge 3 is connected to the peristaltic pump 4 through a pipeline, the output end of the peristaltic pump 4 is connected to the filter cartridge 4 through a pipeline, and the outside of the filter cartridge 4 is connected to a collecting assembly.

[0008] Furthermore, the crushing assembly includes an upper cover, which is fixedly connected to the top of the crushing tank. A motor is installed on the top of the upper cover, and the output end of the motor is fixedly connected to a stirring rod 1 through the upper cover, and the outside of the stirring rod 1 is fixedly connected to multiple crushing blades.

[0009] Furthermore, the outside of the stirring rod 1 is fixedly connected to a connecting rod, both ends of the connecting rod are rotatably connected to gears, the inner side of the upper cover is fixedly connected to a gear ring, and the inner side of the gear ring has two gears with outer sides meshed with each other.

[0010] Furthermore, the bottom of the gear is fixedly connected to a second stirring rod, and the outside of the second stirring rod is fixedly connected to an auger blade.

[0011] Furthermore, the pore size of the filter membrane inside the first filter cartridge does not exceed 30 microns, and the pore size of the filter membrane inside the second filter cartridge does not exceed 5 microns.

[0012] Furthermore, the pore size of the filter membrane inside the filter cartridge three does not exceed 0.8 microns, and the pore size of the filter membrane inside the filter cartridge four does not exceed 0.22 microns.

[0013] Furthermore, the collection assembly includes a collection box, which is fixedly connected to the top of the base. A connecting pipe is fixedly connected to the top of the collection box, and the other end of the connecting pipe is fixedly connected to the bottom through hole of the filter cartridge four.

[0014] Furthermore, a feed port is fixedly connected to the top of the upper cover.

[0015] The utility model has the following beneficial effects:

[0016] 1. In the present invention, after the tissue fragments are crushed, the extract can be filtered layer by layer under the action of the filter cartridge 1 and the filter cartridge 2, thereby fully removing impurities contained in the extract and improving the purity of the separated exosomes. This provides high-purity plant exosomes for subsequent use, improves the quality of the product, and improves the efficiency of exosome separation.

[0017] 2. In the present invention, the motor drives the stirring rod to rotate, so that the multiple crushing blades on the stirring rod crush the plant tissue fragments, so that the extract containing exosomes falls into the inner bottom of the crushing tank. At the same time, the auger blades continuously rotate to transport the crushed plant tissue fragments, so that the plant tissue can be more fully crushed, greatly improving the utilization rate of the raw materials and accelerating the extraction efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a three-dimensional diagram of a plant exosome separation device proposed in the present invention;

[0019] Figure 2 This is a schematic diagram of the crushing tank structure of a plant exosome separation device proposed in the present invention;

[0020] Figure 3 This is a schematic diagram of the gear ring structure of a plant exosome separation device proposed in the present utility model.

[0021] Legend:

[0022] 1. Base; 2. Crushing tank; 3. Peristaltic pump 1; 4. Filter cartridge 1; 5. Peristaltic pump 2; 6. Filter cartridge 2; 7. Peristaltic pump 3; 8. Filter cartridge 3; 9. Peristaltic pump 4; 10. Filter cartridge 4; 11. Connecting pipe; 12. Collection box; 13. Filter screen; 14. Upper cover; 15. Motor; 16. Gear ring; 17. Stirring rod 1; 18. Connecting rod; 19. Gear; 20. Stirring rod 2; 21. Auger blade; 22. Crushing blade; 23. Feed inlet. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0024] Reference Figure 1, the utility model provides an embodiment: a plant exosome separation device, including a base 1, which can be the basic support structure of the entire plant exosome separation device, has a stable and reliable characteristic, and can ensure that the device remains stable during operation, a filtering component is provided on the top right side of the base 1, and the filtering component is used to filter and separate the exosomes, a crushing tank 2 is fixedly connected to the top left side of the base 1, and a filter screen 13 is provided inside the crushing tank 2, which can play a role of preliminary filtering and can block larger plant residues to prevent them from entering the subsequent filtering component and affecting the filtering effect, and a crushing component is provided inside the crushing tank 2 near the inner side of the filter screen 13, and the crushing component is used to crush plant fragments;

[0025] Reference Figure 1 The filtration component includes a peristaltic pump 3, which is fixedly connected to the top of the base 1. The input end of the peristaltic pump 3 is connected to the bottom through hole of the crushing tank 2. The function of the peristaltic pump 3 is to extract the extract containing exosomes from the crushing tank 2 and transport it to the subsequent filtration link. The output end of the peristaltic pump 3 is connected to the filter cartridge 4 through a pipeline, which can ensure that the extract enters the filter cartridge 4 at a uniform flow rate to avoid adverse effects on the filtration process. The filter cartridge 4 can filter out larger particles of impurities, such as incompletely crushed plant tissue fragments, etc. The bottom of the filter cartridge 4 is connected to a peristaltic pump 2 5 through a pipeline. The peristaltic pump 2 5 also has a stable flow control function, which can ensure the smooth transfer of the extract between different filtration stages. The output end of the peristaltic pump 2 5 is connected to the filter cartridge 2 6 through a pipeline, which can further remove more fine impurities, make the extract purer, and lay the foundation for subsequent fine filtration. The bottom of the filter cartridge 2 6 is connected to a peristaltic pump 3 7 through a pipeline. The stable delivery function of the peristaltic pump 3 7 ensures the continuity of the filtration process. The output end of the peristaltic pump 37 is connected to the filter cartridge 38 through a pipeline, which can filter out even smaller impurities, thereby further reducing the impurity content in the extract and improving the purity of the exosomes. The bottom of the filter cartridge 38 is connected to the peristaltic pump 49 through a pipeline, and the output end of the peristaltic pump 49 is connected to the filter cartridge 410 through a pipeline. The filter cartridge 410 can remove almost all tiny impurities, so that the filtered extract contains only high-purity plant exosomes. The outside of the filter cartridge 410 is connected to a collection component, and the filter inside the filter cartridge 4 The membrane pore size does not exceed 30 microns, the filter membrane pore size inside the filter cartridge 2 6 does not exceed 5 microns, the filter membrane pore size inside the filter cartridge 3 8 does not exceed 0.8 microns, and the filter membrane pore size inside the filter cartridge 4 10 does not exceed 0.22 microns. The collection component includes a collection box 12, which is fixedly connected to the top of the base 1. The top of the collection box 12 is fixedly connected to a connecting pipe 11, and the other end of the connecting pipe 11 is fixedly connected to the bottom through hole of the filter cartridge 4 10. The collection of exosomes is achieved through the collection box 12 for subsequent use.

[0026] Specifically, after the crushing is completed, the valve on one side of the bottom of the crushing tank 2 is opened, and the extract containing exosomes enters the filter cartridge 1 4 under the extraction action of the peristaltic pump 1 3. After the primary filtration in the filter cartridge 1 4, larger particles of impurities, such as incompletely crushed plant tissue fragments, are filtered out. Then, the extract enters the filter cartridge 2 6 for secondary filtration under the transportation of the peristaltic pump 2 5, which can further remove more fine impurities and make the extract purer. Subsequently, after being transported by the peristaltic pump 3 7, the extract enters the filter cartridge 3 8 for tertiary filtration, filtering out even finer impurities, so that the impurity content in the extract is further reduced. The purity of the exosomes is improved. Finally, under the transportation of the peristaltic pump 24 9, the extract enters the filter cartridge 24 10 to complete the final filtration, which can remove almost all tiny impurities, so that the filtered extract contains only high-purity plant exosomes. Finally, the filtered extract enters the collection box 12 through the connecting tube 11, thereby realizing the collection of high-purity plant exosomes. Through this multi-stage layer-by-layer filtration method, the impurities contained in the extract can be fully removed, the purity of the separated exosomes is improved, and high-purity plant exosomes are provided for subsequent use, while improving the utilization rate of raw materials.

[0027] Reference Figure 2 and Figure 3 The crushing component includes an upper cover 14, which is fixedly connected to the top of the crushing tank 2 to seal the crushing tank 2, preventing plant tissue from splashing out during the crushing process and ensuring the safety of operation. A motor 15 is installed on the top of the upper cover 14 as a power source for the crushing component to provide powerful power for the crushing of plant tissue fragments. The output end of the motor 15 passes through the upper cover 14 and is fixedly connected to a stirring rod 17. The outside of the stirring rod 17 is fixedly connected to a plurality of crushing blades 22. The crushing blades 22 rotate at a high speed under the drive of the stirring rod 17, thereby crushing the plant tissue fragments. The outside of the stirring rod 17 is fixedly connected to a connecting rod 18, and both ends of the connecting rod 18 are rotating. A gear 19 is connected, and a gear ring 16 is fixedly connected to the inner side of the upper cover 14. The outer sides of the two gears 19 on the inner side of the gear ring 16 are meshed and connected. Through the cooperation between the gear ring 16 and the gear 19, the connecting rod 18 drives the gear 19 to move, and the gear 19 can rotate. The bottom of the gear 19 is fixedly connected to a stirring rod 20, and the outside of the stirring rod 20 is fixedly connected to an auger leaf 21. The function of the auger leaf 21 is to make the plant tissue constantly turn over in the crushing tank 2, to ensure that the plant tissue can be fully crushed, to improve the utilization rate of the raw materials, and also to contribute to the uniform distribution of the extract, so that the exosomes can be better released into the extract. The top of the upper cover 14 is fixedly connected to a feed port 23.

[0028] Specifically, when performing exosome extraction, the plant tissue fragments need to be crushed. First, the plant tissue fragments are put into the filter 13 inside the crushing tank 2 through the feed port 23, and then the motor 15 is started. The operation of the motor 15 drives the stirring rod 17 to rotate, so that the multiple crushing blades 22 on the stirring rod 17 rotate at high speed, and the plant tissue fragments are strongly crushed. In this process, an extract containing exosomes is produced. After preliminary filtration by the filter 13, the extract falls into the inner bottom of the crushing tank 2. At the same time, when the stirring rod 17 rotates, it drives the connecting rod 18 to rotate together, and then the gears 19 at both ends of the connecting rod 18 move. Since the gear 19 is engaged with the gear ring 16 on the inner side of the upper cover 14, the gear 19 will rotate during the movement, and drive the stirring rod 2 20 and the auger blade 21 on the stirring rod 20 to rotate. The auger blade 21 continuously transports the crushed plant tissue fragments, so that the plant tissue can be more fully crushed, greatly improving the utilization rate of the raw materials.

[0029] Working principle: When using this device, first put the plant tissue fragments into the filter 13 inside the crushing tank 2 through the feed port 23, and then start the motor 15. When the motor 15 is running, it will drive the stirring rod 17 to rotate, and then the stirring rod 17 will drive the multiple crushing blades 22 to rotate, crushing the plant tissue fragments, thereby producing an exosome extract, which will fall into the bottom of the crushing tank 2 after being filtered through the filter 13, and the stirring rod 17 will drive the connecting rod 18 to rotate when rotating, and then the connecting rod 18 will drive the two gears 19 to move. At this time, the gear 19 will rotate when moving due to its meshing with the gear ring 16, and will drive the auger blade 21 to rotate, so that it will continuously crush the broken plant tissue fragments. The transportation can make the crushing more complete, thereby improving the utilization rate of the raw materials. After the crushing is completed, the valve on the bottom side of the crushing tank 2 can be opened, so that the extract containing exosomes enters the filter cartridge 1 4 under the extraction of the peristaltic pump 1 3, and enters the filter cartridge 2 6 under the transportation of the peristaltic pump 2 5 after the first-level filtration, and then enters the filter cartridge 3 8 under the transportation of the peristaltic pump 3 7 to realize the tertiary filtration, and then enters the filter cartridge 4 10 through the transportation of the peristaltic pump 4 9 to complete the final filtration, and finally enters the collection box 12 through the connecting pipe 11 to realize the collection of the exosomes. Through multi-stage layer-by-layer filtration, the impurities contained in the extract can be fully removed, thereby obtaining high-purity plant exosomes, which is convenient for subsequent use.

[0030] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent replacements for some of the technical features therein. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A plant exosome separation device, comprising a base (1), characterized in that: A filter assembly is provided on the right side of the top of the base (1), and the filter assembly is used to filter and separate exosomes. A crushing tank (2) is fixedly connected to the left side of the top of the base (1), and a filter screen (13) is provided inside the crushing tank (2). A crushing assembly is provided inside the crushing tank (2) near the inner side of the filter screen (13), and the crushing assembly is used to crush plant fragments. The filtering assembly comprises a peristaltic pump (3), which is fixedly connected to the top of the base (1), an input end of the peristaltic pump (3) is connected to the bottom through hole of the crushing tank (2), an output end of the peristaltic pump (3) is connected to a filter cartridge (4) through a pipeline, the bottom of the filter cartridge (4) is connected to a peristaltic pump (5) through a pipeline, an output end of the peristaltic pump (5) is connected to a filter cartridge (6) through a pipeline, the bottom of the filter cartridge (6) is connected to a peristaltic pump (7) through a pipeline, an output end of the peristaltic pump (7) is connected to a filter cartridge (8) through a pipeline, the bottom of the filter cartridge (8) is connected to a peristaltic pump (9) through a pipeline, an output end of the peristaltic pump (9) is connected to a filter cartridge (10) through a pipeline, and the outside of the filter cartridge (10) is connected to a collecting assembly.

2. The plant exosome separation device according to claim 1, characterized in that: The crushing assembly comprises an upper cover (14), the upper cover (14) being fixedly connected to the top of the crushing tank (2), a motor (15) being installed on the top of the upper cover (14), an output end of the motor (15) passing through the upper cover (14) and being fixedly connected to a stirring rod (17), and a plurality of crushing blades (22) being fixedly connected to the outside of the stirring rod (17).

3. The plant exosome separation device according to claim 2, characterized in that: The outside of the stirring rod (17) is fixedly connected to a connecting rod (18), and both ends of the connecting rod (18) are rotatably connected to gears (19). The inner side of the upper cover (14) is fixedly connected to a gear ring (16), and the inner side of the gear ring (16) is meshed with the outer sides of two gears (19).

4. A plant exosome separation device according to claim 3, characterized in that: The bottom of the gear (19) is fixedly connected to a second stirring rod (20), and the outside of the second stirring rod (20) is fixedly connected to an auger blade (21).

5. The plant exosome separation device according to claim 1, characterized in that: The pore size of the filter membrane inside the filter cartridge 1 (4) does not exceed 30 microns, and the pore size of the filter membrane inside the filter cartridge 2 (6) does not exceed 5 microns.

6. The plant exosome separation device according to claim 1, characterized in that: The pore size of the filter membrane inside the filter cartridge three (8) does not exceed 0.8 microns, and the pore size of the filter membrane inside the filter cartridge four (10) does not exceed 0.22 microns.

7. The plant exosome separation device according to claim 1, characterized in that: The collecting assembly comprises a collecting box (12), wherein the collecting box (12) is fixedly connected to the top of the base (1), and a connecting pipe (11) is fixedly connected to the top of the collecting box (12), and the other end of the connecting pipe (11) is fixedly connected to the bottom through hole of the filter cartridge (10).

8. The plant exosome separation device according to claim 2, characterized in that: The top of the upper cover (14) is fixedly connected with a feed port (23).

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