Cryopreservation equipment for long-distance transfer of stem cells

Abstract

The utility model provides cryopreservation equipment for long-distance transfer of stem cells, and relates to the technical field of stem cell cryopreservation equipment.The cryopreservation equipment comprises a freezing box used for rotating the stem cells for a long distance, and an adjusting assembly used for conveniently taking and placing the stem cells is arranged in the freezing box; the adjusting assembly comprises a movable disc which is movably mounted in the freezing box through a rotating shaft; according to the technical scheme, a micro motor drives two adjusting plates on a movable shaft to rotate, the two adjusting plates respectively extrude and push a corresponding touch plate and a movable plate to move upwards, and the touch plate pushes a supporting plate to drive stem cells in a storage barrel to move upwards; the movable plate drives the sealing plate connected with the connecting support to rotate outwards firstly by means of meshing of the toothed plate and the gear, so that movement of the storage barrel and the sealing plate is not interfered, the storage barrel moves to the outside of the refrigerator through the material hole while the sealing plate moves outwards, and the movement of the storage barrel and the sealing plate can be kept consistent. And the condition of cold air leakage caused by long-time expansion of the material holes is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell freezing equipment, in particular to a long-distance transfer freezing equipment for stem cells. Background Art

[0002] Stem cell transplantation therapy involves transplanting healthy stem cells into the patient's body to repair or replace damaged cells or tissues, thereby achieving the purpose of cure. Stem cell transplantation therapy has a wide range of uses and can generally treat neurological diseases, immune system diseases, and other internal and external diseases. In order to ensure the quality of stem cell products, they need to be refrigerated and transported.

[0003] As shown in the patent document CN216627276U, an intelligent embryonic stem cell freezer, this existing technology can drive the stem cell storage tubes in the placement slot to rotate by coordinating the driving motor, the storage seat, and the rotating shaft. Then, through the coordinating arrangement of the electric telescopic rod, the placement plate, the push rod, and the return spring, the stem cell storage tubes in the placement slot can be driven to move upward, so that the stem cell storage tubes can pass through the second partition, thereby achieving the effect of removing a single stem cell storage tube.

[0004] However, in the prior art, when taking out and placing stem cells, the through-holes on the storage box are always exposed, thereby causing the cold air in the storage box to leak out, thereby affecting the freezing effect of the storage box on the stem cells. Utility Model Content

[0005] In order to overcome the shortcomings of the existing technology, the embodiment of the present application provides a cryopreservation device for long-distance transfer of stem cells. Through the linkage between the storage cylinder and the sealing plate, the sealing plate automatically opens and closes when the storage cylinder moves up and down, thereby avoiding the situation where the material hole is open for a long time and causes cold air leakage.

[0006] The technical solution adopted by the embodiment of the present application to solve the technical problem is:

[0007] A cryopreservation device for long-distance transfer of stem cells, comprising a freezing chamber for long-distance transfer of stem cells, wherein an adjustment component for conveniently taking and placing stem cells is provided inside the freezing chamber;

[0008] The adjustment assembly includes a movable disk movably mounted inside the freezer via a rotating shaft, a plurality of storage cavities distributed in a circular array are defined on the top of the movable disk, a support plate is provided at the bottom of the storage cavity, a storage tube for storing stem cells is provided on the top of the support plate, and the top of the storage tube passes through the movable disk and extends to the top of the movable disk, and a touch plate is installed at the bottom of each support plate, and the bottom end of the touch plate passes through the movable disk and extends to the bottom of the movable disk;

[0009] A mounting bracket is installed on the rear side of the bottom end of the freezer, and a movable shaft is movably installed on the front side of the top end of the mounting bracket through a bearing. The front end of the movable shaft extends into the interior of the freezer, and adjustment plates are installed on the outside of the front and rear ends of the movable shaft. The top of the adjustment plate located at the front end of the movable shaft contacts the bottom of one of the touch plates.

[0010] Among them, a movable plate is provided on the top of the adjustment plate located at the rear end of the movable shaft, a tooth plate is installed on the top of the movable plate, a fixed shaft is provided on the rear side of the top end of the tooth plate, and both ends of the fixed shaft are provided with fixed plates for connecting to the freezer, and both ends of the fixed shaft are movably connected to the two fixed plates through bearings, and the outside of the fixed shaft is provided with a gear meshing with the tooth plate, and both sides of the gear are provided with connecting brackets sleeved on the fixed shaft, and the same sealing plate is installed on the front side of the top of the two connecting brackets, and a material hole is opened on one side of the sealing plate corresponding to the freezer, and the material hole corresponds to the position of one of the storage barrels up and down.

[0011] In one possible implementation, pillars with T-shaped cross-sections are provided on both sides of the touch panel, the top of the pillars are fixed to the bottom of the movable disk, the outside of the bottom end of the pillars is provided with a support for connecting to the touch panel, and the top of the support is provided with a first spring provided on the outside of the pillars.

[0012] In one possible implementation, positioning columns with a T-shaped cross-section are provided on both sides of the movable plate, a positioning plate for connecting to a freezer is installed on the top of the positioning column, a positioning seat is provided at the bottom of the positioning plate and is sleeved on the outside of the bottom end of the positioning column, and the positioning seat is installed on the side of the movable plate close to the positioning column, and a second spring is provided on the top of the positioning seat and is sleeved on the outside of the positioning column.

[0013] In one possible implementation, a box for storing liquid nitrogen is installed at the bottom of the freezer, a delivery pipe is fixedly connected to one side of the box, and the top end of the delivery pipe extends into the interior of the freezer, and a pump body is installed outside the delivery pipe;

[0014] A temperature sensor is installed inside the freezing box, and a controller installed on the top of the freezing box is provided on the top of the temperature sensor.

[0015] In a possible implementation, a through hole is provided on the top of the box body, a servo motor is installed inside the through hole, and an output shaft of the servo motor passes through the freezing box and is fixed to the rotating shaft on the movable disk.

[0016] In a possible implementation, the front side of the box is fixedly connected to a material pipe, and a solenoid valve is installed on the outside of the material pipe.

[0017] In a possible implementation, the diameter of the support plate is larger than the diameter of the storage cylinder.

[0018] In a possible implementation, a micro motor is installed on the rear side of the top end of the mounting bracket, and the output shaft of the micro motor passes through the mounting bracket and is fixed to the movable shaft.

[0019] In summary, the present invention has at least one of the following beneficial technical effects:

[0020] The two adjustment plates on the movable shaft are driven to rotate by a micro motor, and the two adjustment plates squeeze and push the corresponding touch plates and movable plates to move up respectively. The touch plates push the support plates to drive the stem cells in the storage barrel to move upward, and the movable plate drives the sealing plate connected to the connecting bracket to rotate outward first with the help of the engagement between the tooth plate and the gear, so that the movement of the storage barrel and the sealing plate will not interfere with each other. When the sealing plate moves outward, the storage barrel is moved to the outside of the freezer through the material hole, so as to avoid the situation where the material hole is open for a long time and causes cold air leakage. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0022] Figure 2 This is a side sectional view of the freezer of the present invention;

[0023] Figure 3 For the utility model Figure 2 Enlarged view of part A;

[0024] Figure 4 For the utility model Figure 2 Enlarged view of part B;

[0025] Figure 5 It is a side sectional view of the movable disk of the present utility model.

[0026] Figure numerals: 1. Freezer; 2. Movable plate; 3. Storage cavity; 4. Support plate; 5. Storage tube; 6. Touch plate; 7. Mounting bracket; 8. Movable shaft; 9. Adjustment plate; 10. Moving plate; 11. Tooth plate; 12. Fixed shaft; 13. Fixed plate; 14. Gear; 15. Connecting bracket; 16. Sealing plate; 17. Material hole; 18. Pillar; 19. Support; 20. First spring; 21. Positioning column; 22. Positioning plate; 23. Positioning seat; 24. Second spring; 25. Box body; 26. Delivery pipe; 27. Pump body; 28. Temperature sensor; 29. ​​Controller; 30. Through hole; 31. Servo motor; 32. Material pipe; 33. Solenoid valve; 34. Micro motor. DETAILED DESCRIPTION

[0027] The technical solution in the embodiments of the present application is to solve the problems of the above-mentioned background technology, and the overall idea is as follows:

[0028] This embodiment introduces the specific structure of a cryopreservation device for long-distance transfer of stem cells. Figure 1-Figure 5 As shown, it includes a freezing box 1 for rotating stem cells over a long distance, and an adjustment component for conveniently taking and placing stem cells is provided inside the freezing box 1;

[0029] like Figure 1-4 As shown, the adjustment assembly includes a movable plate 2 movably mounted inside the freezer 1 via a rotating shaft. A plurality of storage cavities 3 arranged in a circular array are defined on the top of the movable plate 2. A support plate 4 is provided at the bottom of the storage cavity 3. A storage tube 5 for storing stem cells is provided on top of the support plate 4. The diameter of the support plate 4 is larger than that of the storage tube 5.

[0030] Among them, by setting the diameter of the support plate 4 to be larger than the diameter of the storage tube 5, the support plate 4 can limit the storage tube 5 to prevent the storage tube 5 from separating from the movable disk 2 during movement, and the top of the storage tube 5 passes through the movable disk 2 and extends to the top of the movable disk 2. A touch plate 6 is installed at the bottom of each support plate 4, and the bottom end of the touch plate 6 passes through the movable disk 2 and extends to the bottom of the movable disk 2;

[0031] like Figure 2 and Figure 4 As shown, a mounting bracket 7 is installed on the rear side of the bottom end of the freezer 1, and a micro motor 34 is installed on the rear side of the top end of the mounting bracket 7. The output shaft of the micro motor 34 passes through the mounting bracket 7 and is fixed to the movable shaft 8. The two adjustment plates 9 on the movable shaft 8 are driven to rotate by the servo motor 31, so that the storage cylinder 5 and the sealing plate 16 can be adjusted. The movable shaft 8 is movably mounted on the front side of the top end of the mounting bracket 7 through a bearing. The front end of the movable shaft 8 extends into the interior of the freezer 1. Adjustment plates 9 are installed on the outside of the front and rear ends of the movable shaft 8. The top of the adjustment plate 9 at the front end of the movable shaft 8 contacts the bottom of one of the touch plates 6.

[0032] A movable plate 10 is provided on the top of the adjustment plate 9 located at the rear end of the movable shaft 8, and a tooth plate 11 is installed on the top of the movable plate 10. A fixed shaft 12 is provided on the rear side of the top end of the tooth plate 11, and both ends of the fixed shaft 12 are provided with fixed plates 13 for connecting to the freezer 1, and both ends of the fixed shaft 12 are movably connected to the two fixed plates 13 through bearings. A gear 14 meshing with the tooth plate 11 is provided on the outside of the fixed shaft 12, and connecting brackets 15 sleeved on the fixed shaft 12 are provided on both sides of the gear 14. The same sealing plate 16 is installed on the front side of the top of the two connecting brackets 15, and a material hole 17 is opened on the side of the sealing plate 16 of the freezer 1, and the material hole 17 corresponds to the position of one of the storage tubes 5 up and down.

[0033] The temperature inside the freezing chamber 1 is monitored by a temperature sensor 28, and the monitoring information is transmitted to a controller 29. When the temperature is lower than a set value, the controller 29 activates the pump 27 to deliver the liquid nitrogen in the chamber 25 to the interior of the freezing chamber 1 through the delivery pipe 26. This allows the temperature inside the freezing chamber 1 to be regulated in real time to ensure the cryopreservation effect of the stem cells.

[0034] At the same time, in order to improve the convenience of taking and placing stem cells, the staff starts the servo motor 31 to drive the movable disk 2 to rotate, so that the stem cells in the storage barrel 5 can be transferred to the material hole 17. After that, the staff drives the micro motor 34 to drive the two adjustment plates 9 on the movable shaft 8 to rotate. The two adjustment plates 9 squeeze and push the corresponding touch plates 6 and movable plates 10 to move upward. The touch plates 6 push the support plate 4 to drive the stem cells in the storage barrel 5 to move upward, and the movable plate 10 drives the sealing plate 16 connected to the connecting bracket 15 to rotate outward first by virtue of the engagement of the tooth plate 11 and the gear 14. In this way, the movement of the storage barrel 5 and the sealing plate 16 will not interfere with each other. When the sealing plate 16 moves outward, the storage barrel 5 is moved to the outside of the freezer 1 through the material hole 17, so as to avoid the material hole 17 being open for a long time and causing cold air leakage;

[0035] When placing stem cells: the storage cylinder 5 will be moved into the freezing chamber 1 before the sealing plate 16 , and the sealing plate 16 will block the material hole 17 after the storage cylinder 5 is moved into the freezing chamber 1 .

[0036] In order to facilitate the automatic reset of the touch panel 6, it is necessary to use the rebound of the first spring 20 to reset it. Figure 4 As shown, both sides of the touch plate 6 are provided with pillars 18 with a T-shaped cross-section, the top of the pillar 18 is fixed to the bottom of the movable disk 2, the bottom end of the pillar 18 is provided with a support 19 for connecting to the touch plate 6, and the top of the support 19 is provided with a first spring 20 sleeved on the outside of the pillar 18. The touch plate 6 pushes the storage tube 5 on the support plate 4 to move upward, thereby stretching and expanding the first spring 20. When the adjustment plate 9 is parallel to the touch plate 6, the first spring 20 rebounds and resets, driving the support plate 4 and the storage tube 5 connected to the touch plate 6 to move downward and reset.

[0037] In order to make the sealing plate 16 automatically reset and facilitate multiple reciprocating operations, such as Figure 4 As shown, positioning columns 21 with T-shaped cross-sections are provided on both sides of the movable plate 10, and a positioning plate 22 for connecting to the freezer 1 is installed on the top of the positioning column 21. A positioning seat 23 is provided at the bottom of the positioning plate 22 and is sleeved on the outside of the bottom end of the positioning column 21. The positioning seat 23 is installed on the side of the movable plate 10 close to the positioning column 21, and a second spring 24 is provided on the top of the positioning seat 23 and is sleeved on the outside of the positioning column 21. When the adjustment plate 9 is parallel to the movable plate 10, the second spring 24 drives the movable plate 10 to rebound and reset. At the same time, the movable plate 10 is driven by the tooth plate 11 to reverse with the gear 14, so that the fixed shaft 12 can be driven to drive the sealing plate 16 to reset, thereby achieving the effect of sealing the material hole 17.

[0038] In order to ensure the appropriate temperature in the freezer 1, it is necessary to monitor the temperature in the freezer 1 in real time, such as Figure 1 and 2As shown, a box 25 for storing liquid nitrogen is installed at the bottom of the freezing box 1. A material pipe 32 is fixedly connected to the front side of the box 25, and a solenoid valve 33 is installed on the outside of the material pipe 32. The connection between the material pipe 32 and the box 25 facilitates the replenishment of liquid nitrogen in the box 25. At the same time, the material pipe 32 is opened and closed by the solenoid valve 33 to prevent leakage. A delivery pipe 26 is fixedly connected to one side of the box 25, and the top end of the delivery pipe 26 extends to the inside of the freezing box 1. A pump body 27 is installed on the outside of the delivery pipe 26; a temperature sensor 28 is installed inside the freezing box 1, and a controller 29 installed on the top of the freezing box 1 is provided on the top of the temperature sensor 28. The temperature inside the freezing box 1 is monitored by the temperature sensor 28, and the controller 29 monitors the information and regulates the liquid nitrogen in the box 25, thereby preventing excessive temperature from affecting the cryopreservation effect of stem cells.

[0039] In order to facilitate the removal of the desired stem cells, the stem cells on the movable plate 2 need to be adjusted accordingly, such as Figure 2 and 5 As shown, a through hole 30 is opened on the top of the box body 25, and a servo motor 31 is installed inside the through hole 30. The output shaft of the servo motor 31 passes through the freezing box 1 and is fixed to the rotating shaft on the movable disk 2. The servo motor 31 drives the movable disk 2 to rotate, so that the storage barrel 5 on the movable disk 2 can be rotated and adjusted, so that the required stem cells can be easily removed.

[0040] Finally, it should be noted that the above embodiments are merely examples for the purpose of illustrating the present invention and are not intended to limit the embodiments. Those skilled in the art will readily appreciate that other variations or modifications based on the above description are possible. It is not necessary and impossible to provide an exhaustive list of all possible embodiments. However, any obvious variations or modifications arising therefrom remain within the scope of protection of the present invention.

Claims

1. A cryopreservation device for long-distance transfer of stem cells, characterized in that: It comprises a freezing box (1) for rotating stem cells over a long distance, wherein the freezing box (1) is provided with an adjustment component for conveniently taking and placing the stem cells; The adjustment component includes a movable disk (2) movably mounted inside the freezer (1) via a rotating shaft, a plurality of storage cavities (3) distributed in a ring array are provided on the top of the movable disk (2), a support plate (4) is provided at the bottom of the inner cavity of the storage cavity (3), a storage cylinder (5) for storing stem cells is provided on the top of the support plate (4), and the top end of the storage cylinder (5) passes through the movable disk (2) and extends to the top of the movable disk (2), and a touch plate (6) is installed at the bottom of each support plate (4), and the bottom end of the touch plate (6) passes through the movable disk (2) and extends to the bottom of the movable disk (2); A mounting bracket (7) is installed on the rear side of the bottom end of the freezer (1), and a movable shaft (8) is movably installed on the front side of the top end of the mounting bracket (7) through a bearing. The front end of the movable shaft (8) extends into the interior of the freezer (1), and adjustment plates (9) are installed on the outside of both the front and rear ends of the movable shaft (8). The top of the adjustment plate (9) located at the front end of the movable shaft (8) contacts the bottom of one of the touch plates (6); The top of the adjustment plate (9) at the rear end of the movable shaft (8) is provided with a movable plate (10), a tooth plate (11) is installed on the top of the movable plate (10), and a fixed shaft (12) is provided on the rear side of the top end of the tooth plate (11). Both ends of the fixed shaft (12) are provided with fixed plates (13) for connecting to the freezer (1), and both ends of the fixed shaft (12) are movably connected to the two fixed plates (13) through bearings. The fixed shaft (12) is externally sleeved with a gear (14) meshing with the tooth plate (11), and both sides of the gear (14) are provided with connecting brackets (15) sleeved on the fixed shaft (12). The front sides of the top ends of the two connecting brackets (15) are provided with the same sealing plate (16), and a material hole (17) is opened on one side of the freezer (1) corresponding to the sealing plate (16), and the material hole (17) corresponds to the position of one of the storage barrels (5) in the upper and lower directions.

2. The long-distance stem cell cryopreservation device according to claim 1, characterized in that: Both sides of the touch panel (6) are provided with a support (18) with a T-shaped cross section. The top of the support (18) is fixed to the bottom of the movable disk (2). The bottom of the support (18) is provided with a support (19) for connecting to the touch panel (6). The top of the support (19) is provided with a first spring (20) which is provided on the outside of the support (18).

3. The cryopreservation device for long-distance transfer of stem cells according to claim 1, characterized in that: Both sides of the movable plate (10) are provided with positioning columns (21) with a T-shaped cross section, and a positioning plate (22) for connecting to the freezer (1) is installed on the top of the positioning column (21), and a positioning seat (23) is provided at the bottom of the positioning plate (22) and is sleeved on the outside of the bottom end of the positioning column (21), and the positioning seat (23) is installed on a side of the movable plate (10) close to the positioning column (21), and a second spring (24) is provided on the top of the positioning seat (23) and is sleeved on the outside of the positioning column (21).

4. The long-distance stem cell cryopreservation device according to claim 1, wherein: A box body (25) for storing liquid nitrogen is installed at the bottom of the freezing box (1), a delivery pipe (26) is fixedly connected to one side of the box body (25), and the top end of the delivery pipe (26) extends into the interior of the freezing box (1), and a pump body (27) is installed outside the delivery pipe (26); A temperature sensor (28) is installed inside the freezer (1), and a controller (29) installed on the top of the freezer (1) is provided on the top of the temperature sensor (28).

5. The long-distance stem cell cryopreservation device according to claim 4, characterized in that: A through hole (30) is provided on the top of the box body (25), and a servo motor (31) is installed inside the through hole (30). The output shaft of the servo motor (31) passes through the freezing box (1) and is fixed to the rotating shaft on the movable disk (2).

6. The cryopreservation device for long-distance transfer of stem cells according to claim 4, characterized in that: The front side of the box (25) is fixedly connected to a material pipe (32), and a solenoid valve (33) is installed outside the material pipe (32).

7. The long-distance stem cell cryopreservation device according to claim 1, characterized in that: The diameter of the support plate (4) is greater than the diameter of the storage cylinder (5).

8. The cryopreservation device for long-distance transfer of stem cells according to claim 1, characterized in that: A micro motor (34) is mounted on the rear side of the top end of the mounting bracket (7), and the output shaft of the micro motor (34) passes through the mounting bracket (7) and is fixed to the movable shaft (8).

Images