Mesenchymal stem cell separator

Abstract

The utility model discloses a mesenchymal stem cell separator, which is applied to the technical field of stem cell separation equipment, and adopts the technical scheme that a separation mechanism comprises a motor, the output end of the motor is fixedly connected with a rotating rod through a coupler, the outer wall of the rotating rod is fixedly sleeved with a driving gear, and the driving gear is connected with a driving motor. The device has the technical effects that a motor drives a rotating rod to rotate, the rotating rod drives a driving gear to rotate, the driving gear drives a plurality of driven gears to rotate, under the action of a bearing, the driven gears drive a placement pipe to rotate, the placement pipe drives a separation pipe to rotate under the action of a rubber bulge, and under the action of centrifugal force, the separation pipe is separated. According to the umbilical cord blood separation device, components in umbilical cord blood in the separation tubes are separated to form a plurality of layers, so that mesenchymal stem cells independently form a layer, then extraction is carried out through the extractor, the device can separate the mesenchymal stem cells in the multiple separation tubes, stem cells in the umbilical cord blood cannot be damaged, the separation efficiency is higher, and the quality is better.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell separation equipment, in particular to a mesenchymal stem cell separator. Background Art

[0002] Mesenchymal stem cells are a type of pluripotent stem cell that possess all the common properties of stem cells, namely, self-renewal and multidirectional differentiation capabilities. They are also widely used clinically. Combined use with hematopoietic stem cells can improve the success rate of transplantation and accelerate hematopoietic reconstitution. After patients receive high-dose chemotherapy, the combined infusion of mesenchymal stem cells and hematopoietic stem cells can significantly accelerate blood cell recovery, with safety and no adverse reactions. Mesenchymal stem cells are found not only in bone marrow, but also in skeletal muscle, periosteum, and trabecular bone. Due to their wide range of tissue types, they are highly valuable for clinical applications.

[0003] Currently, the Chinese utility model patent with the announcement number CN219752295U discloses a mesenchymal stem cell filtration separator, a stirring assembly, including a drive motor and a stirring frame assembly connected thereto, the stirring frame assembly is located in the first chamber and is configured to stir the mixed liquid in the first chamber; a first filter plate and a second filter plate, the first filter plate is located in the first chamber, and the second filter plate is located in the second chamber, and the mesh size of the first filter plate is smaller than the mesh size of the second filter plate, so as to achieve sufficient diversion of the separation liquid to the second filter plate, increase the filtration speed, avoid clogging, and filter out the pure stem cell liquid through the second filter plate and transport it out through the second liquid outlet.

[0004] In the above patented technical solution, the umbilical cord blood is stirred by a stirring assembly, and the stirring blades will damage the stem cells in the umbilical cord blood, resulting in poor quality of stem cell separation and insignificant effect. Moreover, only one stem cell package in the umbilical cord blood can be separated at a time, resulting in poor efficiency. Utility Model Content

[0005] The purpose of the present utility model is to provide a mesenchymal stem cell separator to solve the problem in the above-mentioned background art that umbilical cord blood is stirred by a stirring assembly, but the stirring blades will damage the stem cells in the umbilical cord blood, resulting in poor quality of stem cell separation and unclear effect. In addition, only one stem cell packet in the umbilical cord blood can be separated at a time, resulting in poor efficiency.

[0006] To achieve the above objectives, the present invention provides the following technical solutions: a mesenchymal stem cell separator, comprising a separator body and a separation tube, wherein a separation mechanism is provided in the inner cavity of the separator body, a partition is fixedly connected to the inner wall of the separator body, a plurality of circular holes are formed on the partition at equal intervals, and a flip cover mechanism is provided on the top of the partition at the position of the circular holes;

[0007] The separation mechanism includes a motor, the output end of the motor is fixedly connected to a rotating rod through a coupling, the outer wall of the rotating rod is fixedly sleeved with a driving gear, the driving gear is meshed with a driven gear, a bearing is fixedly embedded in the bottom of the inner cavity of the separator body, and a placement tube is rotatably connected to the bottom of the inner cavity of the separator body through the bearing, and the inner wall of the placement tube is evenly fixedly connected with rubber protrusions.

[0008] The utility model is further configured as follows: the flip cover mechanism includes a U-shaped block fixedly connected to the top of the partition, the inner wall of the U-shaped block is fixedly connected to a damping shaft, the damping shaft is rotatably sleeved with a cover body, and the outer wall of the cover body is fixedly connected to a shift block.

[0009] By adopting the above technical solution, when the separation tube is placed in the placement tube, the shift block is moved, which drives the cover body, and the cover body rotates around the damping shaft until the cover body is in contact with the upper surface of the partition, covering the top of the separation tube. When the separation tube performs centrifugal separation on the stem cells, the umbilical cord blood will not be thrown into other separation tubes, affecting the use. The separation tube can also be isolated from the external environment during the separation process, preventing external pathogens and bacteria from prematurely damaging the stem cells, thereby playing a protective role.

[0010] The utility model is further configured as follows: the motor is fixedly mounted on the bottom of the inner cavity of the separator body by means of bolts.

[0011] By adopting the above technical solution, the motor provides power output to the separation mechanism, so that the separation mechanism can operate normally.

[0012] The present invention is further configured as follows: the number of the flip cover mechanisms is the same as the number of the circular holes, and the flip cover mechanisms are arranged at equal intervals with respect to the center of the partition.

[0013] By adopting the above technical solution, the separation tube in each circular hole is isolated from contact with the outside world during the separation process through the flip cover mechanism.

[0014] The utility model is further configured as follows: the radius of the circular hole opened on the partition is larger than the radius of the separation tube, and the partition is located above the placement tube.

[0015] The above technical solution makes it convenient to place the separation tube in the placement tube through the circular hole opened on the partition. In addition, the bottom of the partition is separated from the top of the placement tube, so that the partition will not hinder the rotation of the placement tube during the rotation process.

[0016] The utility model is further configured as follows: the separation tube passes through the circular hole provided on the partition plate and is firmly placed in the placement tube through the rubber protrusion.

[0017] By adopting the above technical solution, the rubber protrusion is compressible, which makes it convenient to place the separation tube in the placement tube under human power. When the rubber protrusion wants to be restored, it must squeeze the separation tube to fix the separation tube.

[0018] The utility model is further configured as follows: a cover door is rotatably connected to the top of the separator body, and a supporting leg is fixedly connected to the bottom of the separator body.

[0019] By adopting the above technical solution, the separator body is covered by providing a cover door, which can prevent dust or foreign matter in the external environment from entering the interior of the separator body.

[0020] The utility model provides a mesenchymal stem cell separator with the following beneficial effects:

[0021] (1) The utility model drives the rotating rod to rotate by a motor, the rotating rod drives the driving gear to rotate, the driving gear drives multiple driven gears to rotate, under the action of the bearing, the driven gear drives the placement tube to rotate, the placement tube drives the separation tube to rotate under the action of the rubber protrusion, and under the action of centrifugal force, the components of the umbilical cord blood in the separation tube are separated into multiple layers, so that the mesenchymal stem cells are independent into a layer, and then extracted through the extractor. This device can separate the mesenchymal stem cells in multiple separation tubes without damaging the stem cells in the umbilical cord blood, so that the separation efficiency is higher and the quality is better.

[0022] (2) The utility model drives the cover body by shifting the shift block, and the cover body rotates around the damping shaft until the cover body fits the upper surface of the partition, covering the top of the separation tube. When the separation tube performs centrifugal separation on the stem cells, the umbilical cord blood will not be thrown into other separation tubes, affecting the use. The separation tube can also be isolated from the external environment during the separation process, preventing external pathogens and bacteria from prematurely damaging the stem cells, thereby playing a protective role. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a three-dimensional diagram of the utility model;

[0024] Figure 2 This is a top view of the separator body of the utility model;

[0025] Figure 3 This is an isometric sectional view of the present invention;

[0026] Figure 4 This is a three-dimensional diagram of the separation mechanism of the utility model;

[0027] Figure 5 This is a three-dimensional diagram of the partition of the utility model;

[0028] Figure 6 This is a three-dimensional diagram of the placement tube of the utility model;

[0029] Figure 7 For this utility model Figure 2 Enlarged view of point A in the middle.

[0030] In the figure: 1. separator body; 2. partition; 21. round hole; 3. separation mechanism; 31. motor; 32. rotating rod; 33. driving gear; 34. driven gear; 35. placement tube; 36. bearing; 37. rubber protrusion; 4. flip cover mechanism; 41. U-shaped block; 42. damping shaft; 43. cover body; 44. shift block; 5. separation tube. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0032] like Figure 1-7 As shown, the utility model provides a technical solution: a mesenchymal stem cell separator, comprising a separator body 1 and a separation tube 5. A cover door is rotatably connected to the top of the separator body 1, and a support leg is fixedly connected to the bottom of the separator body 1. By setting the cover door to cover the separator body 1, dust or foreign matter in the external environment can be prevented from entering the interior of the separator body 1. A separation mechanism 3 is provided in the inner cavity of the separator body 1. A partition 2 is fixedly connected to the inner wall of the separator body 1. A plurality of circular holes 21 are opened on the partition 2 at equal intervals. A flip cover mechanism 4 is provided at the top of the partition 2 at the position of the circular holes 21.

[0033] The separation mechanism 3 includes a motor 31, which is fixedly installed at the bottom of the inner cavity of the separator body 1 by bolts. The motor 31 provides power output to the separation mechanism 3 so that the separation mechanism 3 can operate normally. The output end of the motor 31 is fixedly connected to the rotating rod 32 through a coupling. The outer wall of the rotating rod 32 is fixedly sleeved with a driving gear 33, and the driving gear 33 is meshed with a driven gear 34. A bearing 36 is fixedly embedded at the bottom of the inner cavity of the separator body 1, and the bottom of the inner cavity of the separator body 1 is rotatably connected to a placement tube 35 through a bearing 36. The radius of the circular hole 21 opened on the partition 2 is larger than the radius of the separation tube 5, and the partition 2 is located at the placement tube 3 5, so that the separation tube 5 can be placed in the placement tube 35 through the circular hole 21 opened on the partition 2. In addition, the bottom of the partition 2 is separated from the top of the placement tube 35, so that the partition 2 will not hinder the rotation of the placement tube 35 during the rotation process. The inner wall of the placement tube 35 is evenly and fixedly connected with a rubber protrusion 37. The separation tube 5 passes through the circular hole 21 opened on the partition 2 and is firmly placed in the placement tube 35 through the rubber protrusion 37. The rubber protrusion 37 is compressible, so that the separation tube 5 can be placed in the placement tube 35 by manpower. When the rubber protrusion 37 is to be restored, it must be squeezed on the separation tube 5 to fix the separation tube 5.

[0034] The flip cover mechanism 4 includes a U-shaped block 41 fixedly connected to the top of the partition 2. The flip cover mechanism 4 is equal in number to the circular holes 21 and is evenly spaced about the center of the partition 2. The flip cover mechanism 4 isolates the separation tube 5 in each circular hole 21 from contact with the outside world during the separation process. The inner wall of the U-shaped block 41 is fixedly connected to a damping shaft 42, which is rotatably sleeved with a cover body 43. The outer wall of the cover body 43 is fixedly connected to a shift block 44.

[0035] When the separation tube 5 is placed in the placement tube 35, the shift block 44 is shifted, which drives the cover 43, and the cover 43 rotates around the damping shaft 42 until the cover 43 is in contact with the upper surface of the partition 2, covering the top of the separation tube 5. When the separation tube 5 performs centrifugal separation on the stem cells, the umbilical cord blood will not be thrown into other separation tubes 5, affecting the use. The separation tube 5 can also be isolated from the external environment during the separation process, preventing external pathogens and bacteria from prematurely damaging the stem cells, thereby playing a protective role.

[0036] Working Principle: During use, the motor 31 drives the rotating rod 32 to rotate, which in turn drives the driving gear 33 to rotate, which in turn drives multiple driven gears 34 to rotate. Under the action of the bearing 36, the driven gear 34 drives the placement tube 35 to rotate, and the placement tube 35 drives the separation tube 5 to rotate under the action of the rubber protrusion 37. Under the action of centrifugal force, the components of the umbilical cord blood in the separation tube 5 are separated into multiple layers, so that the mesenchymal stem cells are independently formed into a layer, which is then extracted by the extractor. This device can separate the mesenchymal stem cells in multiple separation tubes 5 without damaging the stem cells in the umbilical cord blood, making the separation efficiency higher and the quality better.

[0037] By shifting the shift block 44, the shift block 44 drives the cover 43, and the cover 43 rotates around the damping shaft 42 until the cover 43 is in contact with the upper surface of the partition 2, covering the top of the separation tube 5. When the separation tube 5 performs centrifugal separation on the stem cells, the umbilical cord blood will not be thrown into other separation tubes 5, affecting the use. The separation tube 5 can also be isolated from the external environment during the separation process, preventing external pathogens and bacteria from prematurely damaging the stem cells, thereby playing a protective role.

[0038] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A mesenchymal stem cell separator, comprising a separator body (1) and a separation tube (5), characterized in that: A separation mechanism (3) is provided in the inner cavity of the separator body (1); a partition (2) is fixedly connected to the inner wall of the separator body (1); a plurality of circular holes (21) are provided on the partition (2) at equal intervals; and a flip mechanism (4) is provided on the top of the partition (2) at the position of the circular holes (21); The separation mechanism (3) includes a motor (31), the output end of the motor (31) is fixedly connected to a rotating rod (32) through a coupling, the outer wall of the rotating rod (32) is fixedly sleeved with a driving gear (33), and the driving gear (33) is meshedly connected to a driven gear (34), a bearing (36) is fixedly embedded in the bottom of the inner cavity of the separator body (1), and a placement tube (35) is rotatably connected to the bottom of the inner cavity of the separator body (1) through the bearing (36), and the inner wall of the placement tube (35) is evenly fixedly connected with a rubber protrusion (37).

2. The mesenchymal stem cell separator according to claim 1, characterized in that: The flip cover mechanism (4) comprises a U-shaped block (41) fixedly connected to the top of the partition (2); the inner wall of the U-shaped block (41) is fixedly connected to a damping shaft (42); the damping shaft (42) is rotatably sleeved with a cover body (43); and the outer wall of the cover body (43) is fixedly connected to a shift block (44).

3. The mesenchymal stem cell separator according to claim 1, characterized in that: The motor (31) is fixedly mounted on the bottom of the inner cavity of the separator body (1) by means of bolts.

4. The mesenchymal stem cell separator according to claim 1, characterized in that: The flip cover mechanisms (4) are the same in number as the circular holes (21), and are arranged at equal intervals about the center of the partition (2).

5. The mesenchymal stem cell separator according to claim 1, characterized in that: The radius of the circular hole (21) opened on the partition (2) is larger than the radius of the separation tube (5), and the partition (2) is located above the placement tube (35).

6. The mesenchymal stem cell separator according to claim 1, characterized in that: The separation tube (5) passes through the circular hole (21) provided on the partition (2) and is securely placed in the placement tube (35) via the rubber protrusion (37).

7. The mesenchymal stem cell separator according to claim 1, characterized in that: The top of the separator body (1) is rotatably connected to a cover door, and the bottom of the separator body (1) is fixedly connected to a support leg.

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