Cell culture and cell matrix preparation plate

Abstract

The utility model provides a cell culture and cell matrix preparation plate which comprises a preparation plate shell and a plurality of liquid collecting grooves which are formed by sinking one side of the preparation plate shell downwards and arranged in an array mode, a plurality of culture openings are formed in the liquid collecting grooves at intervals, and liquid discharging openings are formed between the culture openings in a penetrating mode. The cell culture and cell matrix preparation plate further comprises a slide matched with the liquid collecting tank, a supporting table used for supporting the slide is arranged in the liquid collecting tank, the height of the supporting table is larger than the height between the bottom of the liquid outlet and the bottom of the culture opening, and the height of the supporting table is larger than the height between the bottom of the liquid outlet and the bottom of the culture opening. The cell culture and cell matrix preparation plate further comprises a limiting cover used for covering the preparation plate shell. According to the cell culture and cell matrix preparation plate disclosed by the utility model, the supporting table and the liquid outlet are arranged, so that the slide is more convenient to take out, and the working efficiency is further improved.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, in particular to a cell culture and cell matrix preparation plate. Background Art

[0002] Cell culture and cell matrix preparation plates are usually used to culture cells. The cell slide is placed inside the preparation plate, and then culture water containing the cells to be cultured is poured in. Under certain conditions and after a period of time, the cultured cells are adsorbed on the slide. The experiment can be carried out by taking out the slide with cells.

[0003] In the prior art, the climbing slide is directly placed into the culture port of the culture plate and fits in with the bottom of the culture port. This arrangement makes it inconvenient to take out the climbing slide, thereby reducing work efficiency. Utility Model Content

[0004] In view of the shortcomings of the existing technology, the purpose of the present invention is to provide a cell culture and cell matrix preparation plate, aiming to solve the technical problem in the existing technology that the climbing sheet is directly attached to the bottom of the culture port, making it inconvenient to remove the climbing sheet.

[0005] In order to achieve the above-mentioned purpose, the present invention is implemented through the following technical solutions: a cell culture and cell matrix preparation plate, comprising a preparation plate shell, and a plurality of liquid collection troughs arranged in an array formed by a downward depression on one side of the preparation plate shell, wherein a plurality of culture ports are arranged at intervals in the liquid collection trough, and a drainage port is provided between each of the culture ports, and the width of the drainage port is smaller than the width of the culture port. The cell culture and cell matrix preparation plate also includes a climbing plate cooperating with the liquid collection trough, and a support platform for supporting the climbing plate is provided in the liquid collection trough, and the height of the support platform is higher than the height between the bottom of the drainage port and the bottom of the culture port trough. The cell culture and cell matrix preparation plate also includes a limiting cover for covering the preparation plate shell.

[0006] Compared with the prior art, the beneficial effects of the present invention are: multiple liquid collection tanks are arranged in an array, and multiple culture ports are arranged in the liquid collection tanks, so as to realize a batch culture function, and a support platform for supporting the climbing slice is arranged in the culture port, so as to avoid the climbing slice from sticking to the bottom of the tank of the culture port, and a drainage tank is arranged between the culture ports. By setting the drainage tank, the culture samples between the liquid collection tanks can flow to each other, and the liquid distribution tank can be used to more conveniently remove the climbing slice, thereby improving work efficiency.

[0007] According to one aspect of the above technical solution, the culture port includes two first culture ports located at both ends of the liquid collection tank, and a plurality of second culture ports arranged between the two first culture ports. A film collection slot is provided on one side of the first culture port, and the film collection slot is arranged opposite to the liquid discharge port.

[0008] According to one aspect of the above technical solution, the bottom of the drainage port is flush with the bottom of the culture port.

[0009] According to one aspect of the above technical solution, the outer diameter of the culture port is rectangular.

[0010] According to one aspect of the above technical solution, the support platform includes four pillars arranged in a 1 / 4 cylindrical shape.

[0011] According to one aspect of the above technical solution, the limiting cover is detachably connected to the preparation plate shell.

[0012] According to one aspect of the above technical solution, the limit cover includes a limit cover body, and sliders are provided near both ends of the limit cover body. A protrusion is provided at one end of the slider, and a sliding groove matching the slider is provided on the preparation plate shell.

[0013] According to one aspect of the above technical solution, the limiting cover is made of polystyrene. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the structure of a cell culture and cell matrix preparation plate in one embodiment of the present invention;

[0015] Figure 2 This is a schematic diagram of the structure of the liquid collecting tank in the cell culture and cell matrix preparation plate in one embodiment of the present invention;

[0016] Figure 3 This is a partial cross-sectional view of a cell culture and cell matrix preparation plate in one embodiment of the present invention;

[0017] Figure 4 for Figure 3 A magnified view of middle A;

[0018] Figure 5 This is a schematic diagram of the limiting cover structure of the cell culture and cell matrix preparation plate in one embodiment of the present utility model;

[0019] Key component symbols: 1-preparation plate housing, 11-liquid collecting tank, 12-culture port, 121-first culture port, 122-second culture port, 13-drain port, 14-support platform, 15-slide trough, 16-slide chute, 2-slide climbing, 3-limiting cover, 31-limiting cover body, 32-slider, 33-bump;

[0020] The following specific embodiments will further illustrate the present invention in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0021] To facilitate understanding of the present invention, a more comprehensive description of the present invention will be provided below with reference to the accompanying drawings. The drawings illustrate several embodiments of the present invention. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and comprehensive understanding of the present invention.

[0022] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be an intermediate element. When an element is referred to as being "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. The terms "vertical," "horizontal," "left," "right," and similar expressions used herein are for illustrative purposes only.

[0023] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention pertains. The terms used herein in the specification of this invention are intended only to describe specific embodiments and are not intended to limit the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0024] See also Figures 1 to 2 , a cell culture and cell matrix preparation plate provided by an embodiment of the present invention includes a preparation plate shell 1, one side of the preparation plate shell 1 is recessed downward to form a plurality of liquid collecting troughs 11, and the plurality of liquid collecting troughs 11 are arranged in an array, the liquid collecting trough 11 includes a plurality of culture ports 12 formed in an array, and a drainage port 13 is arranged between the culture ports 12, the drainage port 13 is connected to two adjacent culture ports 12, the width of the drainage port 13 is smaller than the width of the culture port 12, the cell culture and cell matrix preparation plate also includes a climbing sheet 2 cooperating with the liquid collecting trough 11, a support platform 14 for supporting the climbing sheet 2 is provided in the liquid collecting trough 11, the height of the support platform 14 is higher than the height between the bottom of the drainage port 13 and the bottom of the culture port 12, and the cell culture and cell matrix preparation plate also includes a limiting cover 3 for covering the preparation plate shell 1. In this embodiment, there are 16 liquid collecting tanks 11, and there are 24 culture ports 12 inside each liquid collecting tank 11. It can be understood that there are a total of 384 culture ports 12, which can realize the culture function of a large number of experimental cell slides 2.

[0025] See also Figure 4The accommodating cavity structure of the culture port 12 can be circular or rectangular. Since the culture port 12 needs to be placed in the climbing sheet 2, and in actual experiments, the rectangular climbing sheet 2 is convenient for placement on the laboratory bench, in this embodiment, the culture port 12 is selected to be a rectangular structure. Specifically, the outer diameter of the culture port 12 is rectangular. It should be noted that, in this embodiment, the inner diameter of the culture port 12 is set to 3 mm. It can be understood that the width of the drainage port 13 is less than 3 mm. However, the width of the drainage port 13 cannot be too small. If it is too small, it will be inconvenient to put in the climbing sheet 2 to pick up the tool. Moreover, the width of the drainage port 13 cannot be equal to 3 mm. If it is equal to 3 mm, one side of the climbing sheet 2 will have no limit, which is prone to overlap.

[0026] See also Figure 4 In this embodiment, a support platform 14 for supporting the climbing sheet 2 is provided in the culture port 12, wherein the support platform 14 can be circular or square, and the support platform 14 can also be an integral table top or two or more pillars. In addition, the support platform 14 can be configured to be connected only to the bottom of the culture port 12, or only to the side wall of the culture port 12, or to be connected to both the bottom and the side wall of the culture port 12, etc. It can be understood that the support platform 14 is not limited to the structure and position relationship, and is mainly used to support the climbing sheet 2 and connect the climbing sheet 2 to the bottom of the culture port 12. Partial separation, in this embodiment, the support platform 14 is configured as four 1 / 4 cylinders, which are respectively arranged at the four corners of the culture port 12 with a rectangular mouth, and are connected to the side wall of the culture port 12 and the bottom of the groove at the same time. Preferably, the support platform 14 includes four 1 / 4 cylindrical pillars; this design can not only support the climbing sheet 2, but also facilitate production and processing, and has a good internal space of the culture port 12. In addition, the 1 / 4 circular pillar structure can be well attached to the four corners of the interior of the culture port 12 without gaps, and is not easy to retain culture fluid and cells, which is convenient for later internal cleaning.

[0027] See also Figure 4 In this embodiment, in order to facilitate the removal of the climbing slice 2 from the culture ports 12 located on both sides of the liquid collecting tank 11, the culture ports 12 further include two first culture ports 121 located at both ends of the liquid collecting tank 11, and a plurality of second culture ports 122 located between the two first culture ports 121. A slice taking groove 15 is opened on one side of the first culture port 121. The slice taking groove 15 is arranged opposite to the drainage port 13. By providing the slice taking groove 15 and cooperating with the drainage port 13 on the opposite side, a tool for taking the climbing slice 2 can be provided with a placement space, thereby facilitating the removal of the climbing slice 2 in the side culture port 12.

[0028] In this embodiment, the climbing sheet 2 inside the culture port 12 is taken out by clamping the climbing sheet 2 to the two sides of the climbing sheet 2 through the drainage ports 13 on both sides with a picking tool, thereby taking the climbing sheet 2 out of the culture port 12. It can be understood that the bottom of the drainage port 13 should be lower than the height of the support from the bottom of the culture port 12, so that the picking tool can contact the side wall of the climbing sheet 2; and in this embodiment, the bottom of the drainage port 13 is flush with the bottom of the culture port 12. This design facilitates the mutual circulation of the culture fluid inside the collection tank 11, and also facilitates the later cleaning of the inside of the collection tank 11.

[0029] See also Figure 3 and Figure 5 When the cam 31 is in the closed position, the cam 32 is in the closed position, and the cam 32 is in the closed position, so that the cam 32 can slide in the open position. Specifically, the limiting cover 3 includes a limiting cover body 31, and sliders 32 are provided near both ends of the limiting cover body 31. A protrusion 33 is provided at one end of the slider 32, and a slide groove 16 matching the slider 32 is provided on the preparation plate shell 1; it should be noted that in order to facilitate the observation of the growth of cells inside the culture port 12 and increase the internal transmittance, the limiting cover 3 is set to a transparent material. Furthermore, the limiting cover 3 is made of polystyrene, which has extremely high transparency and a transmittance of more than 90%, which is convenient for observing the cell culture situation.

[0030] In summary, the cell culture and cell matrix preparation plate in the above-mentioned embodiments of the present invention, by providing a support platform in the culture port to support the crawling slice, avoids directly placing the crawling slice into the culture port and directly fitting with the bottom of the culture port, which makes it difficult to take out. At the same time, in order to facilitate the better removal of the crawling slice, a drainage port is directly provided at the culture port. The drainage port not only facilitates the removal of the crawling slice, but also allows the drainage inside the culture port to circulate with each other, and slice retrieval grooves are provided on the culture ports on both sides of the liquid collection tank, so that the crawling slices inside the culture ports on both sides are better taken out. In addition, the preparation plate shell is also equipped with a transparent limiting cover, which covers the upper end of the preparation plate by the limiting cover to reduce external dust pollution, and the transparent limiting cover can facilitate intuitive observation of the growth of internal cells.

[0031] Throughout this specification, reference to terms such as "one embodiment," "some embodiments," "examples," "specific examples," or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0032] The above-described embodiments merely represent several implementations of the present invention. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art would be able to make various modifications and improvements without departing from the spirit of the present invention, and these modifications and improvements fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be determined by the appended claims.

Claims

1. A cell culture and cell matrix preparation plate, characterized in that: It includes a preparation plate shell, and several liquid collection tanks arranged in an array formed by a downward depression on one side of the preparation plate shell, a plurality of culture ports are arranged in the liquid collection tank at intervals, and a drainage port is provided between each culture port, and the width of the drainage port is smaller than the width of the culture port. The cell culture and cell matrix preparation plate also includes a climbing sheet that cooperates with the liquid collection tank, and a support platform for supporting the climbing sheet is provided in the liquid collection tank, and the height of the support platform is higher than the height between the bottom of the drainage port and the bottom of the culture port tank. The cell culture and cell matrix preparation plate also includes a limiting cover for covering the preparation plate shell.

2. The cell culture and cell matrix preparation plate according to claim 1, characterized in that The culture port includes two first culture ports located at both ends of the liquid collecting tank, and a plurality of second culture ports located between the two first culture ports. A film taking slot is opened on one side of the first culture port, and the film taking slot is arranged opposite to the liquid discharge port.

3. The cell culture and cell matrix preparation plate according to claim 1, characterized in that: The bottom of the drainage port is flush with the bottom of the culture port.

4. The cell culture and cell matrix preparation plate according to claim 1, characterized in that The outer diameter of the culture port is rectangular.

5. The cell culture and cell matrix preparation plate according to claim 4, characterized in that: The support platform includes four pillars arranged in a 1 / 4 cylindrical shape.

6. The cell culture and cell matrix preparation plate according to any one of claims 1 to 5, characterized in that: The limiting cover is detachably connected to the preparation plate shell.

7. The cell culture and cell matrix preparation plate according to claim 6, characterized in that: The limiting cover includes a limiting cover body, and sliders are provided near both ends of the limiting cover body. A protrusion is provided at one end of the slider, and a sliding groove matching the slider is provided on the preparation plate shell.

8. The cell culture and cell matrix preparation plate according to claim 7, characterized in that: The limiting cover is made of polystyrene.

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