Bioreactor for efficient suspension culture of plant stem cells

Abstract

The utility model belongs to the field of plant cell culture, and particularly discloses a bioreactor for efficient suspension culture of plant stem cells, which comprises a main body, a base, a culture container arranged at the top of the base, an exhaust port arranged at the top of the culture container, and an air inlet arranged at the bottom of the culture container, the air stone is arranged in the air inlet; the sampling mechanism comprises a sampling pipe arranged on one side of the air inlet, a fixing ring arranged on the periphery of the sampling pipe, a sleeve rotationally arranged on the fixing ring, a discharging opening formed in the bottom of the sampling pipe and a discharging opening formed in the sleeve; according to the utility model, by rotating the sleeve, the discharge port is aligned with the discharge port, so that a culture solution can flow into an external container to complete sampling work, and by arranging the three discharge ports with different sizes, overflow waste is avoided while the sampling speed is ensured, and personnel can conveniently and accurately control the sampling amount.

Description

Technical Field

[0001] The utility model belongs to the field of plant cell culture, and in particular relates to a bioreactor for efficient suspension culture of plant stem cells. Background Art

[0002] Suspension culture of plant stem cells is an important field in plant biotechnology and is widely used in plant propagation, genetic engineering, and drug production.

[0003] A bioreactor is a device that provides a controlled environment to promote cell growth and metabolism; for the suspension culture of plant stem cells, bioreactors can provide more ideal growth conditions.

[0004] However, traditional bioreactors are not convenient for workers to sample culture fluids, and it is difficult to control the sampling volume and sampling speed during sampling. Utility Model Content

[0005] The purpose of the utility model is to provide a bioreactor for efficient suspension culture of plant stem cells, so as to solve the problems raised in the above background technology.

[0006] To achieve the above objectives, the present invention provides the following technical solutions:

[0007] A bioreactor for efficient suspension culture of plant stem cells comprises a main body, a base, a culture container arranged on the top of the base, an exhaust port arranged on the top of the culture container, an air inlet arranged on the bottom of the culture container, and an air bubble stone arranged in the air inlet; a sampling mechanism comprising a sampling tube arranged on one side of the air inlet, a fixing ring arranged on the periphery of the sampling tube, a sleeve rotatably arranged on the fixing ring, a discharge port arranged at the bottom of the sampling tube, and an outlet arranged on the sleeve; and a breathing assistance mechanism arranged at the input end of the air inlet.

[0008] Preferably, three discharge ports are provided, and the three discharge ports are of different sizes. A silicone sealing pad is provided on the inner side of the sleeve outside the discharge port.

[0009] Preferably, an elastic clip is provided at the rear end of the fixing ring on the periphery of the sampling tube, and a fixing bolt is threadedly provided at the opening of the elastic clip.

[0010] Preferably, the breathing assistance mechanism includes an air pump arranged at the input end of the air inlet, a flow meter arranged at the output end of the air pump, a filter arranged at the output end of the flow meter, and a silicone tube arranged between the filter and the air inlet and the flow meter.

[0011] Preferably, a limiting ring is provided on the periphery of the culture container, and a support rod is provided on the bottom of the limiting ring.

[0012] Preferably, a rubber stopper is provided inside the exhaust port, and an exhaust pipe is provided inside the rubber stopper.

[0013] Compared with the prior art, the beneficial effects of the present invention are:

[0014] The utility model completes the sampling work by rotating the sleeve to align the discharge port with the discharge port, so that the culture fluid flows into the external container. By setting three discharge ports of different sizes, the larger discharge port is first aligned with the discharge port when sampling to speed up the sampling speed. When the sampling is nearing the end, the sleeve is rotated to align the smaller discharge port with the discharge port to reduce the sampling speed, thereby ensuring the sampling speed while avoiding overflow and waste, and facilitating personnel to accurately control the sampling amount.

[0015] The utility model uses an air pump to input air into a culture container through a silicone tube to carry out oxygen-dissolved suspension culture of plant cells in the culture liquid. During the process, the air intake flow is monitored by a flow meter, which is convenient for personnel to control. The air is filtered by a filter membrane, and then the air is finely processed using bubble stones to improve the dissolved oxygen effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without inventive work. Among them:

[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0018] Figure 2 It is a rear view of the utility model;

[0019] Figure 3 This is a schematic diagram of the internal structure of the utility model;

[0020] Figure 4 This is a schematic structural diagram of the sampling mechanism of the present utility model.

[0021] In the figure: 1. Main body; 101. Culture container; 102. Exhaust port; 103. Air inlet; 104. Rubber bottle stopper; 105. Exhaust pipe; 106. Limiting ring; 107. Support rod; 108. Base; 109. Bubble stone; 2. Sampling mechanism; 201. Sampling tube; 202. Discharge port; 203. Fixed ring; 2031. Movable ring; 2032. Movable groove; 204. Sleeve; 205. Fixing bolt; 206. Discharge port; 207. Silicone sealing pad; 208. Elastic clip; 3. Breathing assistance mechanism; 301. Air pump; 302. Flow meter; 303. Filter element; 304. Silicone tube. DETAILED DESCRIPTION

[0022] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below with reference to the accompanying drawings.

[0023] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0024] Secondly, the term "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in various places throughout this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0025] As attached Figure 1 To the attached Figure 4 As shown:

[0026] Embodiment 1: This embodiment provides a bioreactor for efficient suspension culture of plant stem cells, including a main body 1, including a base 108, a culture container 101 arranged on the top of the base 108, an exhaust port 102 arranged at the top of the culture container 101, an air inlet 103 arranged at the bottom of the culture container 101, and an air stone 109 arranged in the air inlet 103; a sampling mechanism 2, including a sampling tube 201 arranged on one side of the air inlet 103, a fixing ring 203 arranged on the periphery of the sampling tube 201, a sleeve 204 rotatably arranged on the fixing ring 203, a discharge port 202 arranged at the bottom of the sampling tube 201, and an outlet 206 arranged on the sleeve 204; a breathing assistance mechanism 3, arranged at the input end of the air inlet 103.

[0027] Specifically, three discharge ports 206 are provided, and the three discharge ports 206 have different sizes. A silicone sealing gasket 207 is provided on the inner side of the sleeve 204 outside the discharge port 206 .

[0028] Specifically, an elastic clip 208 is provided at the rear end of the fixing ring 203 on the periphery of the sampling tube 201 , and a fixing bolt 205 is threadedly provided at the opening of the elastic clip 208 .

[0029] As can be seen from the above, when in use, the culture solution and plant tissue are poured into the culture container 101 through the exhaust port 102, and oxygen enters the culture container 101 from the bottom of the culture container 101 through the air inlet 103. During the process, the air is made fine by the air bubble stone 109, so that the plant cells can be suspended and cultured by the dissolved oxygen in the culture solution;

[0030] When sampling is required to determine the pH and nitrogen, phosphorus and potassium content of the culture medium, the container is placed at the bottom of the sampling tube 201 and the sleeve 204 is rotated so that the sleeve 204 rotates on the fixed ring 203 until the discharge port 206 is aligned with the discharge port 202. At this time, the culture medium in the culture container 101 will flow out through the discharge port 202 and the discharge port 206, and then flow into the external container to complete the sampling work. Through the three discharge ports 206 of different sizes, when sampling, the larger discharge port 206 is first aligned with the discharge port 202 to speed up the sampling speed. When the sampling is nearing the end, the sleeve 204 is rotated to align the smaller discharge port 206 with the discharge port 202 to reduce the sampling speed, thereby achieving the goal of avoiding overflow and waste while ensuring the sampling speed, and facilitating the personnel to accurately control the sampling amount.

[0031] Embodiment 2: This embodiment is basically the same as the previous embodiment, except that the breathing assistance mechanism 3 includes an air pump 301 arranged at the input end of the air inlet 103, a flow meter 302 arranged at the output end of the air pump 301, a filter element 303 arranged at the output end of the flow meter 302, and a silicone tube 304 arranged between the filter element 303 and the air inlet 103 and the flow meter 302.

[0032] Specifically, a limiting ring 106 is provided on the periphery of the culture container 101, and a support rod 107 is provided at the bottom of the limiting ring 106. The limiting ring 106 will limit the position of the culture container 101, and the support rod 107 will support the position of the culture container 101 to ensure the stability of the culture container 101.

[0033] Specifically, a rubber stopper 104 is provided inside the exhaust port 102 , and an exhaust pipe 105 is provided inside the rubber stopper 104 .

[0034] As can be seen from the above, the rubber bottle stopper 104 cooperates with the exhaust pipe 105 to prevent external dust from falling into the culture container 101 while ensuring the exhaust operation;

[0035] During operation, the air pump 301 is started, and the air pump 301 inputs air into the culture container 101 through the silicone tube 304 to culture the plant cells in the culture medium with dissolved oxygen. During the process, the air intake flow is monitored by the flow meter 302 to facilitate personnel control. The filter element 303 uses a filter membrane to filter the air and directly pass oxygen.

[0036] This design application is applied to the suspension culture scenario of plant stem cells. The dissolved oxygen effect can be improved through the air pump 301, the filter element 303 and the bubble stone. By setting three discharge ports 206 of different sizes, the sampling speed can be guaranteed while avoiding overflow and waste, making it convenient for personnel to take accurate samples.

[0037] It is important to note that the construction and arrangement of the present application shown in a number of different exemplary embodiments are merely illustrative. Although only a few embodiments are described in detail in this disclosure, it should be readily understood by those who refer to this disclosure that many modifications are possible (e.g., the size, scale, structure, shape and proportion of various elements, and parameter values ​​(e.g., temperature, pressure, etc.), mounting arrangements, use of materials, colors, directional changes, etc.) without departing substantially from the novel teachings and advantages of the subject matter described in this application. For example, an element shown as integrally formed may be composed of multiple parts or elements, the position of the element may be inverted or otherwise changed, and the nature or number or position of the discrete elements may be altered or changed. Therefore, all such modifications are intended to be included within the scope of the present invention. The order or sequence of any process or method steps may be changed or reordered according to alternative embodiments. In the claims, any "means plus function" clause is intended to cover the structure of performing the function described herein, and is not only structurally equivalent but also an equivalent structure. Without departing from the scope of the present invention, other substitutions, modifications, changes and omissions may be made in the design, operating conditions and arrangement of the exemplary embodiments. Therefore, the present invention is not limited to the specific embodiments, but extends to various modifications that still fall within the scope of the appended claims.

[0038] Additionally, in order to provide a concise description of example embodiments, all features of an actual embodiment (ie, those features that are not relevant to the best mode presently contemplated for carrying out the invention or those that are not relevant to implementing the invention) may not be described.

[0039] It will be appreciated that in the development of any actual embodiment, as in any engineering or design project, numerous implementation-specific decisions may be made. Such a development effort may be complex and time-consuming, but will, for those of ordinary skill having the benefit of this disclosure, be a routine undertaking of design, fabrication, and production without undue experimentation.

[0040] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, and all of these should be included in the scope of the claims of the present invention.

Claims

1. A bioreactor for efficient suspension culture of plant stem cells, characterized by: include, The main body (1) comprises a base (108), a culture container (101) arranged on the top of the base (108), an exhaust port (102) arranged on the top of the culture container (101), an air inlet (103) arranged on the bottom of the culture container (101), and an air bubble stone (109) arranged in the air inlet (103); A sampling mechanism (2) comprises a sampling tube (201) arranged on one side of the air inlet (103), a fixing ring (203) arranged on the periphery of the sampling tube (201), a sleeve (204) rotatably arranged on the fixing ring (203), a discharge port (202) arranged at the bottom of the sampling tube (201), and a discharge port (206) arranged on the sleeve (204); A breathing assistance mechanism (3) is provided at the input end of the air inlet (103).

2. A bioreactor for efficient suspension culture of plant stem cells according to claim 1, characterized in that: There are three discharge ports (206) with different sizes. A silicone sealing pad (207) is provided on the inner side of the sleeve (204) outside the discharge port (206).

3. The bioreactor for efficient suspension culture of plant stem cells according to claim 1, characterized in that: An elastic clip (208) is provided at the rear end of the fixing ring (203) on the periphery of the sampling tube (201), and a fixing bolt (205) is threadedly provided at the opening of the elastic clip (208).

4. The bioreactor for efficient suspension culture of plant stem cells according to claim 1, characterized in that: The breathing assistance mechanism (3) comprises an air pump (301) arranged at the input end of the air inlet (103), a flow meter (302) arranged at the output end of the air pump (301), a filter element (303) arranged at the output end of the flow meter (302), and a silicone tube (304) arranged between the filter element (303), the air inlet (103) and the flow meter (302).

5. The bioreactor for efficient suspension culture of plant stem cells according to claim 1, characterized in that: A limiting ring (106) is provided on the periphery of the culture container (101), and a supporting rod (107) is provided at the bottom of the limiting ring (106).

6. The bioreactor for efficient suspension culture of plant stem cells according to claim 1, characterized in that: A rubber bottle stopper (104) is provided inside the exhaust port (102), and an exhaust pipe (105) is provided inside the rubber bottle stopper (104).

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