Instrument for detecting plasma vesicle marker

Abstract

The utility model relates to the technical field of plasma detection, and discloses an instrument for detecting a plasma vesicle marker, which comprises a cell detector, a sample injector door is hinged to one side of the front surface of the cell detector, a bracket table is arranged on the inner side of the sample injector door, a micropore disc bracket is mounted on the bracket table, and the micropore disc bracket is provided with a micropore hole. A plasma filtering assembly is arranged on the outer side of the cell detector, the top of the plasma filtering assembly is communicated with a liquid inlet pipe, the bottom of the plasma filtering assembly is communicated with a liquid extraction pipe, and a plasma diluting assembly is arranged on the outer side of the bottom of the plasma filtering assembly; according to the instrument for detecting the plasma vesicle markers, plasma is filtered through the first filter membrane, the third filter membrane and the third filter membrane in sequence, then harmful immune complexes in the plasma are filtered out, the influence of harmful substances on the detection result is avoided, a diluent can be injected into the filtered plasma along the guide pipe by pressing the injection pipe, and the detection efficiency is improved. Further, the plasma can be diluted.

Description

Technical Field

[0001] The utility model relates to the technical field of plasma detection, in particular to an instrument for detecting plasma vesicle markers. Background Art

[0002] Plasma vesicle biomarkers play a crucial role in medical research, particularly in disease diagnosis and monitoring. These markers primarily refer to extracellular vesicles (EVs) present in plasma. These are natural derivatives released by cells into body fluids and carry biological information from the parent cells, including proteins, lipids, DNA, and RNA. The properties of these vesicles are associated with disease states, and therefore they are being explored as disease biomarkers.

[0003] A variety of instruments are available for detecting plasma vesicle markers. These instruments, based on different technical principles, can perform quantitative and qualitative analysis of plasma vesicles (such as exosomes and microvesicles), as well as marker detection. However, when detecting plasma vesicle markers, the presence of harmful immune complexes in the plasma can affect the detection results, and existing detection instruments do not have the ability to filter plasma. Therefore, further improvement is needed. Utility Model Content

[0004] To solve the above-mentioned problems, the present invention provides the following technical solutions: an instrument for detecting plasma vesicle markers, comprising a cell detector, wherein a sample injector door is hingedly connected to one side of the front of the cell detector, a bracket is provided on the inner side of the sample injector door, a microporous plate bracket is mounted on the bracket, a plasma filtration assembly is provided on the outer side of the cell detector, a liquid inlet tube is connected to the top of the plasma filtration assembly, a liquid extraction tube is connected to the bottom of the plasma filtration assembly, and a plasma dilution assembly is provided on the outer side of the bottom of the plasma filtration assembly.

[0005] As an optimization, the plasma filtration component includes a tank body, a ring groove is opened in the top of the tank body, a sealing cover is arranged in the ring groove, a sleeve is fixedly installed in the top center of the sealing cover, and the sleeve is sleeved on the outside of the liquid inlet pipe, four card grooves are opened in the inner array of the top of the tank body, a sealing gasket is arranged in the ring groove, and a card block is fixedly installed in the outer array of the sealing cover, and a filter membrane is arranged inside the tank body. Plasma is input into the tank body through the liquid inlet pipe and then filtered through the filter membrane.

[0006] As an optimization, the card block is slidably connected along the card slot and rotated to be clamped into the ring groove. By twisting the sealing cover so that the card block corresponds to the card slot, the sealing cover can be easily installed and disassembled. At the same time, after the card block is rotated into the ring groove, it will be tightly pressed on the sealing gasket, thereby improving the sealing performance and avoiding contamination from external impurities.

[0007] As an optimization, a limiting elastic plate is fixedly installed on the bottom array of the sealing cover, and the limiting elastic plate is bent and arranged to fit the inner wall of the tank. The limiting elastic plate presses downward against the edge of the filter membrane, thereby positioning the filter membrane.

[0008] As an optimization, the filter membrane includes two layers of first filter membranes, two layers of second filter membranes and one layer of third filter membrane. The first filter membrane is located at the top layer, the second filter membrane is located at the middle layer, and the third filter membrane is located at the bottom layer. The multiple layers of filter membranes filter the plasma to prevent harmful substances in the plasma from affecting the detection.

[0009] As an optimization, the first filter membrane is a filter membrane that has affinity adsorption effect on liposomes and fibrinogen, the second filter membrane is a filter membrane that has filtering effect on effective immune complexes in the blood, and the third filter membrane is a porous structure filter membrane with a pore size of 0.2 microns to remove various particulate substances with a pore size greater than 0.2 microns, and it uses polyethersulfone material.

[0010] As an optimization, the plasma dilution component includes an injection tube and a catheter that passes into the interior of the tank body. A connector is fixedly installed on the top of the catheter, and the bottom end of the injection tube is inserted into the connector. A one-way valve is installed on the catheter. By pushing the injection tube, an appropriate amount of diluent can be injected into the tank body, thereby diluting the plasma. At the same time, the one-way valve is used to prevent the plasma in the tank from flowing back.

[0011] The beneficial effects of the utility model are:

[0012] 1. The instrument for detecting plasma vesicle markers filters the plasma through the first filter membrane, the third filter membrane, and the third filter membrane in sequence, thereby filtering out harmful immune complexes in the plasma to prevent harmful substances from affecting the test results. Then, pressing the injection tube can inject the diluent into the filtered plasma along the catheter to dilute the plasma.

[0013] 2. The instrument for detecting plasma vesicle markers rotates the card block to the card slot by twisting the sleeve, and then pulls the sleeve upward to drive the card block to slide upward along the card slot, and then the sealing cover is removed, and the filter membrane can be replaced. Similarly, the sealing cover is pressed down and then rotated to rotate the card block into the ring groove, and the sealing gasket is squeezed to improve the sealing performance. At the same time, the limiting elastic plate will also fit the inner wall of the tank body and press against the outer edge of the filter membrane to limit it, thereby facilitating the installation and replacement of the filter membrane. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the structure of the utility model;

[0015] Figure 2 This is a schematic diagram of the plasma filtration and dilution structure of the utility model;

[0016] Figure 3 This is a schematic diagram of the interior of the plasma filtration and dilution structure of the utility model;

[0017] Figure 4 This is a schematic diagram of the sealing cover structure of the utility model;

[0018] Figure 5 This is a schematic diagram of the filter membrane structure of the present utility model.

[0019] In the figure: 1. Cell detector; 2. Sampler door; 3. Microporous plate holder; 4. Plasma filtration assembly; 5. Liquid inlet tube; 6. Liquid extraction tube; 7. Plasma dilution assembly; 8. Tank body; 9. Sealing cover; 10. Sleeve; 11. Card slot; 12. Sealing gasket; 13. Card block; 14. Filter membrane; 15. Limiting elastic plate; 16. First filter membrane; 17. Second filter membrane; 18. Third filter membrane; 19. Injection tube; 20. Catheter; 21. Connector; 22. One-way valve. DETAILED DESCRIPTION

[0020] In the description of this application, it should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application.

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0022] See also Figure 1 An instrument for detecting plasma vesicle markers includes a CytoFLEX LX model cell detector 1. An injector door 2 is hinged on one side of the front of the cell detector 1. A bracket is provided on the inner side of the injector door 2. A microporous plate bracket 3 is installed on the bracket. A plasma filtration component 4 is provided on the outer side of the cell detector 1. The top of the plasma filtration component 4 is connected to a liquid inlet tube 5. The bottom of the plasma filtration component 4 is connected to a liquid extraction tube 6. A plasma dilution component 7 is provided on the outer side of the bottom of the plasma filtration component 4.

[0023] See also Figure 2-4The plasma filtration assembly 4 includes a tank body 8, a ring groove is provided in the top of the tank body 8, a sealing cover 9 is provided in the ring groove, a sleeve 10 is fixedly installed at the top center of the sealing cover 9, and the sleeve 10 is sleeved on the outside of the liquid inlet pipe 5, four card grooves 11 are provided in the inner array of the top of the tank body 8, a sealing gasket 12 is provided in the ring groove, and a card block 13 is fixedly installed in the outer array of the sealing cover 9, the card block 13 is slidably connected along the card groove 11 and rotated to be clamped into the ring groove. By twisting the sealing cover 9 so that the card block 13 corresponds to the card groove 11, the sealing cover 9 can be easily installed and removed. At the same time, after the card block 13 is rotated into the ring groove, it will be tightly pressed on the sealing gasket 12, thereby improving the sealing performance and preventing contamination from external impurities.

[0024] See also Figure 2-4 , a filter membrane 14 is provided inside the tank body 8, and plasma is input into the tank body 8 through the liquid inlet pipe 5, and then the plasma is filtered through the filter membrane 14; a limit elastic plate 15 is fixedly installed in an array on the bottom side of the sealing cover 9, and the limit elastic plate 15 is bent and fitted to the inner wall of the tank body 8, and the limit elastic plate 15 is pressed downward against the edge of the filter membrane 14, which will position the filter membrane 14;

[0025] See also Figure 5 The filter membrane 14 includes two layers of first filter membranes 16, two layers of second filter membranes 17, and one layer of third filter membrane 18. The first filter membrane 16 is located at the top layer, the second filter membrane 17 is located in the middle layer, and the third filter membrane 18 is located at the bottom layer. The multiple filter membranes filter the plasma to prevent harmful substances in the plasma from affecting the detection.

[0026] See also Figure 5 The first filter membrane 16 is a filter membrane with affinity adsorption for liposomes and fibrinogen, the second filter membrane 17 is a filter membrane with filtering effect on effective immune complexes in the blood, and the third filter membrane 18 is a porous structure filter membrane with a pore size of 0.2 microns to remove various particulate matter with a pore size greater than 0.2 microns, and it is made of polyethersulfone material;

[0027] See also Figure 2-3 The plasma dilution component 7 includes an injection tube 19 and a catheter 20 that passes into the interior of the tank body 8. A connector 21 is fixedly installed on the top of the catheter 20. The bottom end of the injection tube 19 is inserted into the connector 21. A one-way valve 22 is installed on the catheter 20. By pushing the injection tube 19, an appropriate amount of diluent can be injected into the tank body 8, thereby diluting the plasma. At the same time, the one-way valve 22 prevents the plasma in the tank body 8 from flowing back.

[0028] During use, plasma is first injected into the tank body 8 through the liquid inlet tube 5, and then the plasma is filtered through the first filter membrane 16, the second filter membrane 17 and the third filter membrane 18 in sequence to prevent harmful substances in the plasma from affecting the test results. Then, the injection tube 19 is pressed to push the internal diluent outward, so that the diluent will be injected into the filtered plasma in the tank body 8 along the conduit 20, that is, the dilution of the plasma is completed. Finally, the filtered and diluted plasma is drawn into the microporous plate holder 3 through the liquid extraction tube 6, and can be tested by the cell detector 1;

[0029] Among them, by twisting the sleeve 10, the sealing cover 9 is driven to rotate, so that the block 13 is rotated to the slot 11, and then the sleeve 10 can be pulled upward to drive the block 13 to slide upward along the slot 11, so that the sealing cover 9 will be taken out upward, and it is convenient to replace the filter membrane 14. During installation, the sealing cover 9 is pressed downward in the same way, and then the sleeve 10 is rotated to drive the block 13 on the outside of the sealing cover 9 to rotate, so that the block 13 is located in the annular groove, and then the sealing cover 9 will be installed and limited, and the sealing gasket 12 will be squeezed to improve the sealing performance. At the same time, the limiting elastic plate 15 will also fit the inner wall of the tank body 8 and press against the outer edge of the filter membrane 14 to limit it.

[0030] In summary, the instrument for detecting plasma vesicle markers filters the plasma through the first filter membrane 16, the third filter membrane 18, and the third filter membrane 18 in sequence, thereby filtering out harmful immune complexes in the plasma to prevent harmful substances from affecting the test results. Then, pressing the injection tube 19 can inject the diluent into the filtered plasma along the catheter 20, thereby diluting the plasma.

[0031] By twisting the sleeve 10 to rotate the block 13 to the slot 11, and then pulling the sleeve 10 upwards, the block 13 can be driven to slide upward along the slot 11, and the sealing cover 9 can be taken out. After that, the filter membrane 14 can be replaced. Similarly, the sealing cover 9 can be pressed downward and rotated to rotate the block 13 into the annular groove, and the sealing gasket 12 will be squeezed to improve the sealing. At the same time, the limiting elastic plate 15 will also fit the inner wall of the tank body 8 and press against the outer edge of the filter membrane 14 to limit it, thereby facilitating the installation and replacement of the filter membrane.

[0032] In the description of this utility model, unless otherwise expressly specified or limited, the terms "disposed," "installed," "connected," "connected," and "fixed" should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection or electrical connection; direct connection or indirect connection through an intermediate medium; internal communication between two components or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.

[0033] The standard parts used in the present invention can all be purchased from the market, and special-shaped parts can all be customized according to the description and the drawings.

[0034] The above are only preferred specific implementation methods of the present invention, but the protection scope of the present invention is not limited to them. Any technician familiar with the technical field can make equivalent replacements or changes based on the technical solution and utility model concept of the present invention within the technical scope disclosed by the present invention, and they should be covered by the protection scope of the present invention.

Claims

1. An instrument for detecting plasma vesicle markers, comprising a cell detector (1), characterized in that: A sample injector door (2) is hingedly connected to one side of the front of the cell detector (1), a bracket is provided on the inner side of the sample injector door (2), a microporous plate bracket (3) is mounted on the bracket, a plasma filter assembly (4) is provided on the outer side of the cell detector (1), a liquid inlet pipe (5) is connected to the top of the plasma filter assembly (4), a liquid extraction pipe (6) is connected to the bottom of the plasma filter assembly (4), and a plasma dilution assembly (7) is provided on the outer side of the bottom of the plasma filter assembly (4).

2. The instrument for detecting plasma vesicle markers according to claim 1, characterized in that: The plasma filtration assembly (4) comprises a tank body (8), a ring groove is provided in the top of the tank body (8), a sealing cover (9) is provided in the ring groove, a sleeve (10) is fixedly installed at the center of the top of the sealing cover (9), and the sleeve (10) is sleeved on the outside of the liquid inlet pipe (5), four card grooves (11) are provided in an array on the inner side of the top of the tank body (8), a sealing gasket (12) is provided in the ring groove, a card block (13) is fixedly installed in an array on the outer side of the sealing cover (9), and a filter membrane (14) is provided inside the tank body (8).

3. The instrument for detecting plasma vesicle markers according to claim 2, characterized in that: The clamping block (13) is slidably connected along the clamping groove (11) and is rotated to be clamped into the annular groove.

4. The instrument for detecting plasma vesicle markers according to claim 2, characterized in that: A limiting elastic plate (15) is fixedly mounted on the bottom array of the sealing cover (9), and the limiting elastic plate (15) is bent and arranged to fit the inner wall of the tank body (8).

5. The instrument for detecting plasma vesicle markers according to claim 2, characterized in that: The filter membrane (14) includes two layers of first filter membranes (16), two layers of second filter membranes (17) and one layer of third filter membrane (18), wherein the first filter membrane (16) is located at the top layer, the second filter membrane (17) is located at the middle layer, and the third filter membrane (18) is located at the bottom layer.

6. The instrument for detecting plasma vesicle markers according to claim 5, characterized in that: The first filter membrane (16) is a filter membrane that has an affinity adsorption effect on liposomes and fibrinogen, the second filter membrane (17) is a filter membrane that has a filtering effect on effective immune complexes in the blood, and the third filter membrane (18) is a porous structure filter membrane with a pore size of 0.2 microns to remove various particulate substances with a pore size greater than 0.2 microns, and it is made of polyethersulfone material.

7. The instrument for detecting plasma vesicle markers according to claim 1, characterized in that: The plasma dilution component (7) includes an injection tube (19) and a conduit (20) that leads into the interior of the tank body (8). A connector (21) is fixedly installed on the top of the conduit (20), and the bottom end of the injection tube (19) is inserted into the connector (21). A one-way valve (22) is installed on the conduit (20).

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