Clamp for space omics transfer glass slide pretreatment

Abstract

The utility model discloses a clamp for space omics transfer glass slide pretreatment. The clamp comprises an upper pressing module, a lower positioning module and a movable sealing module, wherein the lower positioning module is positioned below the upper pressing module and is suitable for placing a glass slide; the movable sealing module is detachably mounted on the upper pressing module; the movable sealing module is located between the upper pressing module and the lower positioning module and comprises a sliding seat set and a silica gel sealing gasket suitable for being installed in the sliding seat set in an interference mode, the sliding seat set is movably installed on the upper pressing module, and the silica gel sealing gasket protrudes out of the sliding seat set and is suitable for surrounding the periphery of tissue on the slide. According to the clamp for space omics transfer glass slide pretreatment, by arranging the silica gel sealing gasket protruding out of the sliding seat set, tissue on a glass slide can be located in the range of the silica gel sealing gasket, and when the pressing module is pressed, it is guaranteed that the silica gel sealing gasket is pressed on the glass slide, so that the silica gel sealing gasket is sealed around the tissue; therefore, the reagent can be prevented from overflowing from a gap between the silica gel sealing gasket and the slide during subsequent dropping of the reagent.

Description

Technical Field

[0001] The utility model relates to the technical field of spatial transcription, in particular to a clamp used for pre-processing of spatial omics transfer slides. Background Art

[0002] Spatial omics transfer technology involves transferring tissue slice analytes directly from a sample slide to a capture slide equipped with capture probes, which is then used for subsequent library construction and sequencing experiments. The initial steps in this experimental process require the addition of a fixed amount of reagents to the tissue on the sample slide for cell pretreatment and probe hybridization to ensure the subsequent capture and imaging quality. However, because tissues on standard slides are often manually scooped up and fixed in random positions, existing tissue transfer devices struggle to meet sealing requirements during the probe hybridization process, making subsequent additions of reagents and samples prone to overflow. Utility Model Content

[0003] Therefore, the technical problem to be solved by the present invention is that the tissue transfer device in the prior art is difficult to meet the sealing requirements during the probe hybridization process and is prone to overflow when the reagent sample is subsequently added, thereby providing a clamp for pre-treatment of spatial omics transfer slides.

[0004] In order to solve the above technical problems, the technical solutions of the present utility model are as follows:

[0005] A fixture for pre-processing of spatial omics transfer slides, comprising an upper pressing module, a lower positioning module located below the upper pressing module and suitable for placing a slide, and a movable sealing module detachably mounted on the upper pressing module; the movable sealing module is located between the upper pressing module and the lower positioning module, and comprises a slide group and a silicone sealing gasket suitable for interference fitting in the slide group, the slide group being movably mounted on the upper pressing module, the silicone sealing gasket protruding from the slide group, and suitable for surrounding the tissue on the slide.

[0006] Furthermore, the slide group includes an X-axis slide and a Y-axis slide, both of which are through-through in the middle, the silicone sealing gasket is interference-mounted in the middle of the Y-axis slide, the X-axis slide is suitable for sliding installation on the upper pressure module, and is suitable for moving along the X-axis; the Y-axis slide is suitable for sliding installation in the middle of the X-axis slide, and is suitable for moving along the X-axis.

[0007] Furthermore, the upper pressure module includes an upper pressure plate, two clamping connecting plates and two locking plates; the two clamping connecting plates are respectively rotatably installed on opposite sides of the upper pressure plate, and the two locking plates are respectively rotatably installed on the side of the clamping connecting plate away from the upper pressure plate.

[0008] Furthermore, a protruding side plate is provided on the inner wall through which the upper pressure plate passes, a boss is provided on the X-axis slide, the boss is suitable for being slidably connected to the side plate, the X-axis slide is located in the inner ring of the side plate, and is suitable for sliding along the X-axis in the inner ring of the side plate.

[0009] Furthermore, a notch is provided at one end of the X-axis slide, and the Y-axis slide is suitable for being detachably mounted on the X-axis slide through the notch.

[0010] Furthermore, two limiting plates are provided on opposite sides of the upper pressure plate, and the clamping connecting plate is rotatably connected between the corresponding two limiting plates.

[0011] Furthermore, the lower positioning module includes a lower positioning plate and two spring seats respectively installed at opposite ends of the lower positioning plate, the two spring seats are both protruding from the lower positioning plate, and the upper pressure module is suitable for being installed between the two spring seats.

[0012] Furthermore, positioning pins are provided on opposite ends of the lower positioning plate, and positioning holes corresponding to the positioning pins are opened on opposite ends of the upper pressure module.

[0013] Furthermore, spring holes are provided on opposite ends of the lower positioning plate, a spring is provided in the spring hole, and a spring pin suitable for extending out of the spring hole is connected to the spring.

[0014] Furthermore, the spring hole is set to be through, one end of the spring is fixedly installed on the spring seat, and the spring pin is connected to the other end of the spring.

[0015] The technical solution of this utility model has the following advantages:

[0016] 1. The fixture provided by the present invention is used for the pre-processing of spatial omics transfer slides. A movable sealing module is set between the upper pressing module and the lower positioning module. The movable sealing module is detachably connected to the upper pressing module, so that the movable sealing module can be quickly removed from the upper pressing module, and the movable sealing module can be cleaned separately. A silicone sealing gasket protruding from the slide assembly is set, so that the tissue on the glass slide can be within the range of the silicone sealing gasket. When the lower pressing module is pressed, the silicone sealing gasket is pressed tightly against the glass slide, so that the silicone sealing gasket is sealed around the tissue, thereby preventing the reagent from overflowing from the gap between the silicone sealing gasket and the glass slide when the reagent is subsequently added.

[0017] 2. The fixture provided by the present invention is used for the pre-processing of spatial omics transfer slides. The X-axis slide, the Y-axis slide and the silicone sealing gasket are all connected in the middle, so that a sample loading hole is formed on the movable sealing module. By setting a slidable X-axis slide and a Y-axis slide, the movable sealing module can be moved in the X-axis and Y-axis directions, so that the sample loading hole and the tissue on the slide can be accurately aligned, and the operation is simple and convenient.

[0018] 3. The clamp provided by the present invention for pre-processing of spatial omics transfer slides comprises an upper pressing module comprising an upper pressing plate, two clamping connecting plates, and two locking plates; the two clamping connecting plates are rotatably mounted on opposite sides of the upper pressing plate, and the two locking plates are rotatably mounted on the side of the clamping connecting plate away from the upper pressing plate. With this arrangement, after the upper pressing module, movable sealing module, and lower positioning module are installed, the upper pressing module, movable sealing module, and lower positioning module can be fixed in position by rotating the clamping connecting plates and locking plates and pressing the locking plates against the lower positioning module. This allows the three to be fixed without manual pressure, saving effort.

[0019] 4. The fixture provided by the present invention for pre-processing slides for spatial omics transfers comprises a protruding side panel on the inner wall of the upper pressure plate, a boss on the X-axis slide adapted for sliding connection to the side panel, and the X-axis slide located within the inner ring of the side panel and adapted to slide along the X-axis within the inner ring. This arrangement allows the protruding side panel on the inner wall of the upper pressure plate to cooperate with the boss on the X-axis slide, providing guidance and limiting for the X-axis slide as it moves within the inner ring of the upper pressure plate.

[0020] 5. The fixture provided by this invention for pre-processing slides for spatial omics transfers has a notch at one end of the X-axis slide, through which the Y-axis slide can be removably mounted. This arrangement allows for easy removal and installation of the Y-axis slide through the notch, making it easier to clean the slide separately after removal.

[0021] 6. The fixture provided by this invention for pre-processing slides for spatial omics transfers comprises a lower positioning module comprising a lower positioning plate and two spring seats mounted at opposite ends of the lower positioning plate. Both spring seats protrude from the lower positioning plate, and the upper pressing module is adapted to be mounted between the two spring seats. This arrangement allows the upper pressing module to be initially positioned and limited by the two protruding spring seats.

[0022] 7. The fixture provided by this invention for pre-processing slides for spatial omics transfers features positioning pins on opposite ends of the lower positioning plate, and positioning holes corresponding to the pins on opposite ends of the upper pressing module. This arrangement allows the upper pressing module to be aligned with the lower positioning plate through the corresponding positioning pins and holes, achieving rapid and precise positioning, ensuring rapid coordination between the upper pressing module and the lower positioning plate, and simultaneously securing the upper pressing module to the lower positioning plate, preventing displacement that could affect subsequent experimental results.

[0023] 8. The fixture provided by the present invention for pre-processing slides for spatial omics transfers has spring holes on opposite ends of the lower positioning plate, each containing a spring, which is connected to a spring pin adapted to extend out of the spring hole. With this arrangement, when a slide with tissue is placed on the lower positioning plate, the upper pressing plate will not directly contact the lower positioning plate due to the support provided by the spring pin. After adjusting the position of the movable sealing module, the upper pressing module is pressed downward, while simultaneously rotating the downward clamping connecting plate and the locking plate. This allows the silicone sealing gasket to tightly seal around the tissue, thereby preventing spillage during subsequent reagent additions.

[0024] 9. The fixture provided by this invention for pre-processing spatial omics transfer slides features a spring hole extending through it. One end of the spring is fixedly mounted on the spring seat, and the spring pin is connected to the other end of the spring. This arrangement ensures the stability of the spring and prevents it from detaching from the lower positioning module due to its elastic potential energy. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0026] Figure 1 This is an exploded view of the fixture provided by the utility model for pre-processing of spatial omics transfer slides;

[0027] Figure 2 This is a three-dimensional structural diagram of the upper pressure module of the utility model;

[0028] Figure 3 This is an exploded view of the movable sealing module in the present utility model;

[0029] Figure 4 This is an exploded view of the upper positioning module in the present utility model.

[0030] Explanation of the accompanying reference numerals: 1. Upper pressure module; 11. Upper pressure plate; 12. Clamping connecting plate; 13. Locking plate; 14. Limiting plate; 2. Movable sealing module; 21. X-axis slide; 22. Y-axis slide; 23. Silicone sealing gasket; 3. Lower positioning module; 31. Lower positioning plate; 32. Spring pin; 33. Spring; 34. Spring seat; 35. Positioning pin; 4. Glass slide. DETAILED DESCRIPTION

[0031] The following is a clear and complete description of the technical solution of the present invention in conjunction with the accompanying drawings. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0032] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating positions or relationships, are based on the positions or relationships shown in the accompanying drawings and are intended solely to facilitate the description of this utility model and simplify the description. They do not indicate or imply that the devices or components referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0033] In the description of this utility model, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections; direct connections, indirect connections through an intermediate medium, and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.

[0034] In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0035] like Figure 1-4The clamp shown is used for pre-processing of spatial omics transfer slides, including an upper pressing module 1, a lower positioning module 3 located below the upper pressing module 1 and suitable for placing a glass slide 4, and a movable sealing module 2 detachably mounted on the upper pressing module 1; the movable sealing module 2 is located between the upper pressing module 1 and the lower positioning module 3, and includes a slide group and a silicone sealing gasket 23 suitable for interference installation in the slide group. The slide group is movably mounted on the upper pressing module 1, and the silicone sealing gasket 23 protrudes from the slide group and is suitable for being circled around the tissue on the glass slide 4.

[0036] This fixture for pre-processing of spatial omics transfer slides is characterized by arranging a movable sealing module 2 between the upper pressing module 1 and the lower positioning module 3, and the movable sealing module 2 is detachably connected to the upper pressing module 1, so that the movable sealing module 2 can be quickly removed from the upper pressing module 1 and the movable sealing module 2 can be cleaned separately; by arranging a silicone sealing gasket 23 protruding from the slide group, the tissue on the glass slide 4 can be placed within the range of the silicone sealing gasket 23, and when the lower pressing module is pressed, the silicone sealing gasket 23 can be pressed tightly against the glass slide 4, so that the silicone sealing gasket 23 is sealed around the tissue, thereby preventing the reagent from overflowing from the gap between the silicone sealing gasket 23 and the glass slide 4 when the reagent is subsequently added.

[0037] In this embodiment, the upper pressure module 1 includes an upper pressure plate 11, two clamping plates 12, and two locking plates 13. The two clamping plates 12 are rotatably mounted on opposite sides of the upper pressure plate 11, and the two locking plates 13 are rotatably mounted on the side of the clamping plates 12 away from the upper pressure plate 11. With this arrangement, after the upper pressure module 1, the movable sealing module 2, and the lower positioning module 3 are installed, the upper pressure module 1, the movable sealing module 2, and the lower positioning module 3 can be fixed in position by rotating the clamping plates 12 and locking plates 13 and pressing the locking plates 13 against the lower positioning module 3. This allows the three to be fixed manually without manual pressure, which is more labor-saving.

[0038] Specifically, the clamping connecting plate 12 is rotatably connected to the upper pressing plate 11 via a cylindrical pin. Specifically, two limit plates 14 are fixedly provided on opposite sides of the upper pressing plate 11, and the clamping connecting plate 12 is rotatably connected between the corresponding two limit plates 14 via a cylindrical pin.

[0039] In this embodiment, the slide assembly includes an X-axis slide 21 and a Y-axis slide 22, both of which are through-holes. A silicone sealing gasket 23 is interference-fitted in the middle of the Y-axis slide 22. The X-axis slide 21 is adapted to be slidably mounted on the upper pressing module 1 and adapted to move along the X-axis; the Y-axis slide 22 is adapted to be slidably mounted in the middle of the X-axis slide 21 and adapted to move along the X-axis. With this arrangement, since the X-axis slide 21, the Y-axis slide 22, and the silicone sealing gasket 23 are all through-holes in the middle, a sample loading hole is formed on the movable sealing module 2. By providing the slidable X-axis slide 21 and Y-axis slide 22, the movable sealing module can be moved in the X-axis and Y-axis directions, thereby enabling the sample loading hole to be precisely aligned with the tissue on the slide 4, and operation is simple and convenient.

[0040] In this embodiment, a protruding side plate is provided on the inner wall of the upper pressing plate 11, and a boss is provided on the X-axis slide 21. The boss is adapted to be slidably connected to the side plate. The X-axis slide 21 is located within the inner ring of the side plate and is adapted to slide along the X-axis within the inner ring of the side plate. This arrangement allows the protruding side plate on the inner wall of the upper pressing plate 11 to cooperate with the boss on the X-axis slide 21, thereby providing guidance and limiting for the X-axis slide 21 as it moves within the inner ring of the upper pressing plate 11.

[0041] In this embodiment, a notch is provided at one end of the X-axis slide 21, and the Y-axis slide 22 is adapted to be detachably mounted on the X-axis slide 21 through the notch. This arrangement allows the Y-axis slide 22 to be easily disassembled and installed through the notch, making it easy to clean the Y-axis slide 22 separately after disassembly.

[0042] In this embodiment, the lower positioning module 3 includes a lower positioning plate 31 and two spring seats 34 mounted at opposite ends of the lower positioning plate 31. The two spring seats 34 protrude from the lower positioning plate 31, and the upper pressing module 1 is adapted to be mounted between the two spring seats 34. This arrangement allows the upper pressing module 1 to be initially positioned and limited by the two protruding spring seats 34.

[0043] In this embodiment, locating pins 35 are provided at opposite ends of the lower positioning plate 31, and locating holes corresponding to the locating pins 35 are provided at opposite ends of the upper pressure module 1. This arrangement allows the upper pressure module 1 to be aligned with the lower positioning plate 31 through the corresponding locating pins 35 and locating holes, achieving rapid and precise positioning, ensuring rapid engagement between the upper pressure module 1 and the lower positioning plate 31, and simultaneously securing the upper pressure module 1 to the lower positioning plate 31 to prevent displacement that could affect subsequent experimental test results.

[0044] In this embodiment, spring holes are formed at opposite ends of the lower positioning plate 31. Springs 33 are disposed within the spring holes, and spring pins 32 are connected to the springs 33, extending out of the spring holes. This arrangement prevents the upper pressing plate 11 from directly contacting the lower positioning plate 31 due to the support provided by the spring pins 32 when the tissue-bearing slide 4 is placed on the lower positioning plate 31. After adjusting the position of the movable sealing module 2, the upper pressing module 1 is pressed downward, while simultaneously rotating the downward clamping connecting plate 12 and the locking plate 13. This allows the silicone sealing gasket 23 to tightly seal around the tissue, thereby preventing spillage during subsequent reagent additions.

[0045] Specifically, the spring hole is through-set, one end of the spring 33 is fixedly mounted on the spring seat 34, and the spring pin 32 is connected to the other end of the spring 33. This arrangement can ensure the stability of the spring 33 and prevent the spring 33 from detaching from the lower positioning module 3 under elastic potential energy.

[0046] Instructions for use: Place the glass slide 4 with tissue into the lower positioning plate 31, and then align the upper pressing module 1 with the positioning pin 35 and gently place it on the lower positioning plate 31. Due to the supporting effect of the spring pin 32, the upper pressing plate 11 will not directly fit with the lower positioning plate 31. Then adjust the X-axis slide 21 and the Y-axis slide 22 on the movable sealing module 2 to allow the tissue to be within the range of the silicone sealing gasket 23, and then press down the upper pressing module 1, and at the same time press down the clamping connecting plate 12 and the locking plate 13. Since the thickness of the silicone sealing gasket 23 is greater than the gap between the upper pressing plate 11 and the lower positioning plate 31, the silicone sealing gasket 23 will be deformed at this time, so that the silicone sealing gasket 23 can seal around the tissue, thereby preventing overflow when the reagent is added later.

[0047] To sum up, this fixture for pre-processing of spatial omics transfer slides, by setting a movable sealing module 2 between the upper pressing module 1 and the lower positioning module 3, and the movable sealing module 2 is detachably connected to the upper pressing module 1, so that the movable sealing module 2 can be quickly removed from the upper pressing module 1, and the movable sealing module 2 can be cleaned separately; by setting a silicone sealing gasket 23 protruding from the slide group, the tissue on the glass slide 4 can be placed within the range of the silicone sealing gasket 23, and when the lower pressing module is pressed, the silicone sealing gasket 23 can be pressed tightly against the glass slide 4, so that the silicone sealing gasket 23 is sealed around the tissue, thereby preventing the reagent from overflowing from the gap between the silicone sealing gasket 23 and the glass slide 4 when the reagent is subsequently added.

[0048] Obviously, the above embodiments are merely examples for clarity of explanation and are not intended to limit the implementation methods. Those skilled in the art will readily appreciate that other variations or modifications based on the above descriptions are possible. It is not necessary and impossible to enumerate all implementation methods here. Obvious variations or modifications arising therefrom remain within the scope of protection of the present invention.

Claims

1. A fixture for pre-treatment of spatial omics transfer slides, characterized in that: The invention comprises an upper pressing module (1), a lower positioning module (3) located below the upper pressing module (1) and suitable for placing a glass slide (4), and a movable sealing module (2) detachably mounted on the upper pressing module (1); the movable sealing module (2) is located between the upper pressing module (1) and the lower positioning module (3), and comprises a slide group and a silicone sealing gasket (23) suitable for interference mounting in the slide group; the slide group is movably mounted on the upper pressing module (1); the silicone sealing gasket (23) protrudes from the slide group and is suitable for surrounding the tissue on the glass slide (4).

2. The fixture for pre-treatment of spatial omics transfer slides according to claim 1, characterized in that: The slide assembly comprises an X-axis slide (21) and a Y-axis slide (22) both of which are through-through in the middle. The silicone sealing gasket (23) is interference-mounted in the middle of the Y-axis slide (22). The X-axis slide (21) is suitable for being slidably mounted on the upper pressure module (1) and suitable for moving along the X-axis. The Y-axis slide (22) is suitable for being slidably mounted in the middle of the X-axis slide (21) and suitable for moving along the X-axis.

3. The fixture for pre-treatment of spatial omics transfer slides according to claim 2, characterized in that: The upper pressure module (1) comprises an upper pressure plate (11), two clamping connection plates (12) and two locking plates (13); the two clamping connection plates (12) are respectively rotatably mounted on opposite sides of the upper pressure plate (11), and the two locking plates (13) are respectively rotatably mounted on a side of the clamping connection plate (12) away from the upper pressure plate (11).

4. The fixture for pre-treatment of spatial omics transfer slides according to claim 3, characterized in that: A protruding side plate is provided on the inner wall of the upper pressure plate (11), and a boss is provided on the X-axis slide (21). The boss is suitable for being slidably connected to the side plate. The X-axis slide (21) is located in the inner ring of the side plate and is suitable for sliding along the X-axis in the inner ring of the side plate.

5. The fixture for pre-treatment of spatial omics transfer slides according to claim 4, characterized in that: A notch is provided at one end of the X-axis slide (21), and the Y-axis slide (22) is adapted to be detachably mounted on the X-axis slide (21) through the notch.

6. The fixture for pre-treatment of spatial omics transfer slides according to claim 3, characterized in that: Two limiting plates (14) are provided on opposite sides of the upper pressing plate (11), and the clamping connecting plate (12) is rotatably connected between the corresponding two limiting plates (14).

7. The fixture for pre-treatment of spatial omics transfer slides according to claim 1, characterized in that: The lower positioning module (3) comprises a lower positioning plate (31) and two spring seats (34) respectively installed at opposite ends of the lower positioning plate (31), the two spring seats (34) are both protruding from the lower positioning plate (31), and the upper pressing module (1) is suitable for being installed between the two spring (33) seats.

8. The fixture for pre-treatment of spatial omics transfer slides according to claim 7, characterized in that: Positioning pins (35) are provided on opposite ends of the lower positioning plate (31), and positioning holes corresponding to the positioning pins (35) are provided on opposite ends of the upper pressure module (1).

9. The fixture for pre-treatment of spatial omics transfer slides according to claim 7, characterized in that: The lower positioning plate (31) is provided with spring holes at both opposite ends. A spring (33) is provided in the spring hole. The spring (33) is connected with a spring pin (32) suitable for extending out of the spring hole.

10. The fixture for pre-treatment of spatial omics transfer slides according to claim 9, characterized in that: The spring hole is through-set, one end of the spring (33) is fixedly mounted on the spring seat (34), and the spring pin (32) is connected to the other end of the spring (33).

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