DNA (deoxyribonucleic acid) fluorescent PCR (polymerase chain reaction) detection card box
By designing a simple DNA fluorescence PCR detection cartridge, the problems of complexity and high cost of molecular diagnostic POCT devices were solved, the detection process was simplified and the manufacturing cost was reduced, which promoted the industrialization of molecular diagnostic POCT.
Patent Information
- Application Number
- CN202421964921.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-13
- Publication Date
- 2025-09-09
- Estimated Expiration
- 2034-08-13
AI Technical Summary
The existing molecular diagnostic POCT devices are complex in design, which increases the difficulty and cost of production and manufacturing, making it difficult to achieve industrialization.
A DNA fluorescent PCR detection cartridge is designed, comprising a cartridge body, PCR tubes, and a partition. It has a simple structure and can perform fluorescent PCR detection by simply adding sample cells. Cell lysis, magnetic bead transfer, and fluorescent PCR reaction are completed by the detector.
It simplifies the detection process, reduces manufacturing costs and the difficulty of reagent packaging, and enables the industrialization of molecular diagnostic POCT, making it suitable for large-scale promotion and application.
Smart Images
Figure CN223316685U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of DNA detection, in particular to the technical field of DNA fluorescence PCR detection, and specifically refers to a DNA fluorescence PCR detection card box. Background Art
[0002] POCT, point-of-care testing, also known as on-site rapid testing, refers to a testing method that is performed at the sampling site and uses portable analytical instruments and supporting reagents to quickly obtain test results.
[0003] The development of POCT aligns with the concept of "big health" and promotes the establishment of a patient-centered system. Under new healthcare models such as tiered diagnosis and treatment, telemedicine, mobile healthcare, and shared healthcare, POCT is experiencing unprecedented growth opportunities. POCT is characterized by its simplicity and speed.
[0004] For molecular diagnostics, point-of-care testing (POCT) aims to simplify the complex steps of nucleic acid testing while accelerating testing rates and shortening reporting times. However, beyond meeting these requirements, cost control remains a key prerequisite for the success of POCT, particularly given the complex designs of existing POCT systems, which significantly increase manufacturing challenges.
[0005] Therefore, it is hoped to provide a DNA fluorescence PCR detection device that can perform fluorescence PCR detection by simply adding sample cells, with a simple structure and easy manufacturing, which reduces manufacturing costs and the difficulty of subsequent reagent packaging, so that molecular diagnostic POCT can truly be industrialized. Utility Model Content
[0006] In order to overcome the shortcomings of the above-mentioned prior art, one purpose of the present invention is to provide a DNA fluorescent PCR detection card box, which only needs to add sample cells to perform fluorescent PCR detection, and has a simple structure and is easy to manufacture, which reduces manufacturing costs and the difficulty of subsequent reagent packaging, allowing molecular diagnostic POCT to truly achieve industrialization and be suitable for large-scale promotion and application.
[0007] To achieve the above objectives, the present invention provides a DNA fluorescent PCR detection cartridge, which is characterized by comprising a cartridge body, a PCR tube, and a partition, wherein:
[0008] The box body is arranged vertically and along the left-right direction, the top of the box body is open, the partition is arranged vertically and along the front-back direction, the number of the partitions is 7, and the 7 partitions are arranged in the box body at intervals from left to right, thereby dividing the internal space of the box body into a lysis liquid storage chamber, a first silicone oil storage chamber, a first washing liquid storage chamber, a second silicone oil storage chamber, a second washing liquid storage chamber, a third silicone oil storage chamber, a third washing liquid storage chamber and a fourth silicone oil storage chamber from left to right, the PCR tube is arranged vertically and is located under the box body and is connected to the box body, the inner cavity of the PCR tube is connected to the fourth silicone oil storage chamber, and a notch is provided on the front side of the partition along the left-right direction.
[0009] Preferably, the notch is located in the lower middle portion of the front side of the partition.
[0010] More preferably, the notch is located at 1 / 3 to 1 / 2 of the height of the partition.
[0011] Preferably, the notch is rectangular in shape.
[0012] More preferably, the notch is rectangular in shape.
[0013] Furthermore, the length of the notch is 2 mm to 3 mm, and the width of the notch is 1 mm to 2 mm.
[0014] Preferably, the cross-section of the box body is in the shape of a rectangle with the left side convex in an arc shape toward the left.
[0015] Preferably, the cross-section of the lysis liquid storage chamber is circular, and the cross-section of the first silicone oil storage chamber, the cross-section of the first washing liquid storage chamber, the cross-section of the second silicone oil storage chamber, the cross-section of the second washing liquid storage chamber, the cross-section of the third silicone oil storage chamber, the cross-section of the third washing liquid storage chamber and the cross-section of the fourth silicone oil storage chamber are all rectangular.
[0016] Preferably, the DNA fluorescent PCR detection card box also includes a sample loading tube and a sealing cover, the sample loading tube is vertically arranged and located on the lysis solution storage chamber and is arranged on the top of the box body and the leftmost partition to connect to the lysis solution storage chamber, and the sealing cover is arranged on the sample loading tube.
[0017] More preferably, the sealing cover is sleeved and threadedly engaged with the sample addition tube.
[0018] The beneficial effects of the present invention are mainly:
[0019] The DNA fluorescent PCR detection card box of the present invention includes a box body, a PCR tube and a partition. The box body is arranged vertically and along the left and right directions, and the top is open. The partition is arranged vertically and along the front and back directions. There are 7 partitions. The 7 partitions are arranged in the box body at intervals from left to right, thereby dividing the internal space of the box body into a lysis solution storage chamber, a first silicone oil storage chamber, a first washing liquid storage chamber, a second silicone oil storage chamber, a second washing liquid storage chamber, a third silicone oil storage chamber, a third washing liquid storage chamber and a fourth silicone oil storage chamber from left to right. The PCR tube is arranged vertically and is located under the box body and is connected to the box body. The inner cavity of the PCR tube is connected to the fourth silicone oil storage chamber. A notch is provided on the front side of the partition along the left and right directions. Therefore, it only needs to add sample cells to perform fluorescent PCR detection. The structure is simple and the manufacturing is simple, which reduces the manufacturing cost and reduces the difficulty of later reagent packaging, so that molecular diagnostic POCT can be truly industrialized and suitable for large-scale promotion and application.
[0020] These and other objects, features and advantages of the present invention are fully reflected in the following detailed description and drawings, and can be achieved by the means, devices and their combinations specifically pointed out in the content of the utility model. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 This is a three-dimensional decomposition diagram of a specific embodiment of the DNA fluorescent PCR detection cartridge of the utility model. Figure 1 .
[0022] Figure 2 yes Figure 1 The three-dimensional exploded diagram of the specific embodiment shown Figure 2 .
[0023] Figure 3 yes Figure 1 The front exploded schematic diagram of the specific embodiment shown.
[0024] Figure 4 yes Figure 1 The schematic perspective view of the liquid filling condition of the PCR tube of the specific embodiment shown is a three-dimensional diagram, wherein the cover is not shown.
[0025] Figure 5 It is adopted Figure 1 The illustrated embodiment is a three-dimensional perspective schematic diagram of the movement path of the magnetic rod (magnetic bead) during detection.
[0026] (Explanation of Symbols)
[0027] 1. Box body; 2. PCR tube; 3. Partition; 4. Lysis solution storage chamber; 5. First silicone oil storage chamber; 6. First washing solution storage chamber; 7. Second silicone oil storage chamber; 8. Second washing solution storage chamber; 9. Third silicone oil storage chamber; 10. Third washing solution storage chamber; 11. Fourth silicone oil storage chamber; 12. Notch; 13. Sample tube; 14. Cap; 15. PCR reaction solution; 16. Paraffin; 17. Primer probe droplet. DETAILED DESCRIPTION
[0028] In order to more clearly understand the technical content of the present invention, the following embodiments are given to explain in detail.
[0029] In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they cannot be understood as limitations on the present invention.
[0030] See Figures 1 to 3 As shown, in a specific embodiment of the present invention, the DNA fluorescent PCR detection card box of the present invention includes a box body 1, a PCR tube 2 and a partition 3, wherein:
[0031] The box body 1 is arranged vertically and along the left-right direction. The top of the box body 1 is open. The partition 3 is arranged vertically and along the front-back direction. There are 7 partitions 3. The 7 partitions 3 are arranged in the box body 1 at intervals from left to right, thereby dividing the internal space of the box body 1 into a lysis liquid storage chamber 4, a first silicone oil storage chamber 5, a first washing liquid storage chamber 6, a second silicone oil storage chamber 7, a second washing liquid storage chamber 8, a third silicone oil storage chamber 9, a third washing liquid storage chamber 10 and a fourth silicone oil storage chamber 11 from left to right. The PCR tube 2 is arranged vertically and is located under the box body 1 and is connected to the box body 1. The inner cavity of the PCR tube 2 is connected to the fourth silicone oil storage chamber 11. The front side of the partition 3 is provided with a notch 12 along the left-right direction.
[0032] The front side of the partition 3 is provided with a notch 12 along the left-right direction. The notch 12 may be provided at any suitable position on the front side of the partition 3 along the left-right direction. Figures 1 and 2 As shown, in a specific embodiment of the present invention, the notch 12 is located in the lower middle portion of the front side of the partition 3 .
[0033] The notch 12 is located in the lower middle part of the front side of the partition 3 and can be located at any suitable position in the lower middle part of the front side of the partition 3. In a specific embodiment of the present invention, the notch 12 is located at 1 / 3 to 1 / 2 of the height of the partition 3.
[0034] The notch 12 may have any suitable shape. Preferably, the notch 12 is rectangular. Figures 1 and 2 As shown, in a specific embodiment of the present invention, the notch 12 is rectangular in shape.
[0035] The length and width of the notch 12 can be determined as needed. Furthermore, the length of the notch 12 is 2 mm to 3 mm, and the width of the notch 12 is 1 mm to 2 mm. In a specific embodiment of the present invention, the length of the notch 12 is 3 mm, and the width of the notch 12 is 1 mm.
[0036] The cross-sectional profile of the box body 1 can have any suitable shape, see Figures 1 to 3 As shown, in a specific embodiment of the present invention, the cross-sectional profile of the box body 1 is a rectangular shape with the left side convex in an arc shape toward the left.
[0037] The cross-sections of the lysis solution storage chamber 4, the first silicone oil storage chamber 5, the first washing solution storage chamber 6, the second silicone oil storage chamber 7, the second washing solution storage chamber 8, the third silicone oil storage chamber 9, the third washing solution storage chamber 10, and the fourth silicone oil storage chamber 11 may have any suitable shape. Figures 1 and 2 As shown, in a specific embodiment of the present invention, the cross-section of the lysis liquid storage chamber 4 is circular, and the cross-sections of the first silicone oil storage chamber 5, the first washing liquid storage chamber 6, the second silicone oil storage chamber 7, the second washing liquid storage chamber 8, the third silicone oil storage chamber 9, the third washing liquid storage chamber 10 and the fourth silicone oil storage chamber 11 are all rectangular.
[0038] The box body 1 and the partition 3 can be a single component, formed in one piece, or can be two components connected to each other. In a specific embodiment of the present invention, the box body 1 and the partition 3 are formed in one piece.
[0039] The box body 1 and the PCR tube 2 can be a single component, formed in one piece, or can be two components connected to each other. In a specific embodiment of the present invention, the box body 1 and the PCR tube 2 are formed in one piece.
[0040] The DNA fluorescent PCR detection cartridge may also include any other suitable components, see Figures 1 to 3 As shown, in a specific embodiment of the present invention, the DNA fluorescent PCR detection card box also includes a sample addition tube 13 and a cover 14. The sample addition tube 13 is vertically arranged and located on the lysis solution storage chamber 4 and is arranged on the top of the box body 1 and the leftmost partition 3 to connect to the lysis solution storage chamber 4. The cover 14 is covered on the sample addition tube 13.
[0041] The sealing cover 14 is provided on the sample tube 13 and can adopt any suitable structure. Figures 1 to 3 As shown, in a specific embodiment of the present invention, the sealing cover 14 is sleeved and threadedly engaged with the sample tube 13 .
[0042] When using, first, the test cartridge is loaded with liquid:
[0043] 1. Filling the PCR tube 2 with liquid: First, add the PCR reaction solution 15 into the PCR tube 2, seal it with paraffin 16, then add the primer probe droplets 17, and finally seal the entire tube with paraffin 16, so that the primer probe droplets 17 are sealed in the paraffin 16. Figure 4 shown.
[0044] 2. Add lysis solution (used to lyse sample cells and release DNA), silicone oil, washing solution 1 (washing solution 1 is usually a high-concentration guanidine salt, used to further remove residual protein and lipid impurities), silicone oil, washing solution 2, silicone oil, washing solution 3 (washing solution 2 and washing solution 3 are usually purified water containing BSA and detergent, used to neutralize residual guanidine salt and lipid impurities) and silicone oil to the lysis solution storage chamber 4, the first silicone oil storage chamber 5, the first washing solution storage chamber 6, the second silicone oil storage chamber 7, the second washing solution storage chamber 8, the third silicone oil storage chamber 9, the third washing solution storage chamber 10 and the fourth silicone oil storage chamber 11, respectively. After the paraffin 16 solidifies, seal the top opening of the lysis solution storage chamber 4 with aluminum-plastic film (if the sample tube 13 and the cover 14 are present, cover the cover 14 on the sample tube 13).
[0045] The following are the steps to use the test cartridge:
[0046] Open the aluminum-plastic film (open the cover 14 if the sample tube 13 and the cover 14 are present), add 10ul of magnetic beads, such as silicon hydroxyl magnetic beads (which can specifically adsorb nucleic acids), into the lysate, and then add the sample, for example, using a pipetting gun to add 200uL to 400uL of sample (such as plasma, serum, etc.), pipet and mix, or insert a sampling tool (such as an oropharyngeal swab, nasopharyngeal swab, etc.), and shake and stir;
[0047] After placing the present invention in the detection position of the detector, the detector heats the side wall of the box body 1 and the PCR tube 2 of the present invention for 5 to 10 minutes;
[0048] The mechanical arm of the detector drives a magnetic rod, which moves from top to bottom on the front side wall of the box body 1 (corresponding to the position of the lysate storage chamber 4), then moves from left to right (corresponding to the position of the notch 12), and then moves downward (corresponding to the position of the PCR tube 2). Figure 5 The magnetic rod moves in the direction of the arrow shown in the figure until it reaches the front wall of the PCR tube 2. The magnetic rod then moves the magnetic beads (the magnetic beads can also be added to the lysis solution after the sample is added) into the PCR tube 2. The magnetic rod then moves up and down several times (for example, 0.5 mm / s, 2 mm, 10 times) to mix with the PCR reaction solution 15 and the primer probe droplets 17 in the PCR tube 2.
[0049] The detector then performs a fluorescent PCR reaction procedure, and then detects the change in the fluorescent signal in the PCR tube 2 to determine whether the sample cells contain the nucleic acid fragment to be detected.
[0050] Therefore, with the present invention, it is only necessary to add the sample cells to the detection cartridge, realize cell lysis in the detection cartridge, and then place the present invention into the detector. The detector uses magnetic rods and magnetic beads to complete the transfer and washing of DNA, and then enters the PCR tube for fluorescent PCR reaction. The detector then detects the change of fluorescence signal in the PCR tube to detect whether the sample cells contain the nucleic acid fragment to be tested. In other words, except for adding the sample cells, other processes are completed by the detector. The detection is simple, convenient and fast, which reduces work intensity and improves work efficiency.
[0051] On the basis of achieving the above functions, the utility model optimizes the design of the detection cartridge from the perspective of production and manufacturing, has a simple structure, reduces the manufacturing cost of the detection cartridge itself (the cartridge body can be integrally formed with the partition and PCR tube, and there is no need to assemble other components inside the cartridge body) and the difficulty of subsequent reagent packaging, and truly realizes the industrialization of molecular diagnostic POCT.
[0052] In summary, the DNA fluorescent PCR detection cartridge of the present invention only needs to add sample cells to perform fluorescent PCR detection. It has a simple structure and is easy to manufacture, which reduces manufacturing costs and the difficulty of subsequent reagent packaging, allowing molecular diagnostic POCT to truly be industrialized and suitable for large-scale promotion and application.
[0053] It can be seen that the purpose of this utility model has been fully and effectively achieved. The functional and structural principles of this utility model have been demonstrated and explained in the embodiments. Without departing from the principles described, the embodiments may be modified in any way. Therefore, this utility model includes all modified embodiments based on the spirit and scope of the claims.
Claims
1. A DNA fluorescent PCR detection cartridge, characterized in that: It includes a box body, PCR tubes and partitions, wherein: The box body is arranged vertically and along the left-right direction, the top of the box body is open, the partition is arranged vertically and along the front-back direction, the number of the partitions is 7, and the 7 partitions are arranged in the box body at intervals from left to right, thereby dividing the internal space of the box body into a lysis liquid storage chamber, a first silicone oil storage chamber, a first washing liquid storage chamber, a second silicone oil storage chamber, a second washing liquid storage chamber, a third silicone oil storage chamber, a third washing liquid storage chamber and a fourth silicone oil storage chamber from left to right, the PCR tube is arranged vertically and is located under the box body and is connected to the box body, the inner cavity of the PCR tube is connected to the fourth silicone oil storage chamber, and a notch is provided on the front side of the partition along the left-right direction.
2. The DNA fluorescent PCR detection cartridge according to claim 1, wherein The notch is located at the lower middle portion of the front side of the partition.
3. The DNA fluorescent PCR detection cartridge according to claim 2, wherein: The notch is located at 1 / 3 to 1 / 2 of the height of the partition.
4. The DNA fluorescent PCR detection cartridge according to claim 1, wherein The notch is in a rectangular shape.
5. The DNA fluorescent PCR detection cartridge according to claim 4, wherein: The notch is in a rectangular shape.
6. The DNA fluorescent PCR detection cartridge according to claim 5, wherein: The length of the notch is 2 mm to 3 mm, and the width of the notch is 1 mm to 2 mm.
7. The DNA fluorescent PCR detection cartridge according to claim 1, wherein The cross-section of the box body is in the shape of a rectangle with the left side convex in an arc shape toward the left.
8. The DNA fluorescent PCR detection cartridge according to claim 1, wherein The cross-section of the lysis liquid storage chamber is circular, and the cross-sections of the first silicone oil storage chamber, the first washing liquid storage chamber, the second silicone oil storage chamber, the second washing liquid storage chamber, the third silicone oil storage chamber, the third washing liquid storage chamber, and the fourth silicone oil storage chamber are all rectangular.
9. The DNA fluorescent PCR detection cartridge according to claim 1, wherein The DNA fluorescent PCR detection card box also includes a sample addition tube and a sealing cover. The sample addition tube is vertically arranged and located on the lysate storage chamber and is arranged on the top of the box body and the leftmost partition to connect with the lysate storage chamber. The sealing cover is arranged on the sample addition tube.
10. The DNA fluorescent PCR detection cartridge according to claim 9, wherein: The sealing cover is sleeved and threadedly engaged with the sample addition tube.