Multi-cell co-culture device

Abstract

The utility model discloses a multi-cell co-culture device. The multi-cell co-culture device comprises a shell, a supporting plate is fixedly installed at the bottom of the shell, the supporting plate is annular, a first baffle is arranged in the supporting plate, a plurality of upper chambers are detachably arranged at the upper end of the supporting plate, the upper chambers are installed on a fixing ring, and diaphragms are arranged at the bottoms of the upper chambers; the edge of the fixing ring can slide between the supporting plate and the shell. According to the multi-cell co-culture device provided by the utility model, the co-culture of different types of cells and another type of specific cells is realized by arranging the plurality of upper chambers, and compared with the existing one-to-one culture mode, the co-culture of different types of cells and specific cells can be completed by adopting one small culture chamber, so that the operation is convenient.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a multi-cell co-culture device. Background Art

[0002] Multi-cell co-culture is an important experimental method in fields such as cell biology and tissue engineering. It simulates the interactions and communications between cells in the body by culturing different types of cells together in the same environment. Compared with traditional single cell culture, multi-cell co-culture can better reflect the complex conditions in real organisms and help to gain a deeper understanding of cell functions and disease mechanisms.

[0003] Multicellular co-culture systems mainly include two methods: direct contact and indirect contact. Direct contact co-culture usually involves directly mixing different cells in a certain proportion and then culturing them. It is suitable for studying the mechanism of direct contact between cells, while indirect contact co-culture includes conditioned medium co-culture and embedded co-culture. The former uses shared culture medium to study the interaction between cells through secretions, while the latter uses specially designed co-culture systems (such as Transwell chambers) to prevent direct contact between cells while allowing the exchange of substances in the culture medium.

[0004] Existing co-cultivation chambers generally consist of a single above-ground chamber and a lower chamber. However, in the case of a large number of experimental groups, multiple co-cultivation chambers need to be set up to complete the corresponding experiments, which is relatively cumbersome.

[0005] Therefore, it is necessary to provide a multi-cell co-culture device to solve the above technical problems. Utility Model Content

[0006] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a multi-cell co-culture device capable of completing multiple groups of experiments in one co-culture device.

[0007] In order to achieve the above purpose, the technical solution adopted by the present utility model is as follows:

[0008] A multi-cell co-culture device comprises: a shell, a support plate fixedly mounted at the bottom of the shell, the support plate being annular, a first baffle being provided inside the support plate, a plurality of upper chambers being detachably provided at the upper end of the support plate, the upper chambers being mounted on a fixed ring, a diaphragm being provided at the bottom of the upper chambers, and the edge of the fixed ring being able to slide between the support plate and the shell.

[0009] Preferably, a central chamber is further provided at the bottom of the shell, an additional chamber is provided at the upper end of the central chamber, a second baffle is provided in the additional chamber, and the second baffle divides the internal space of the additional chamber into two parts.

[0010] Preferably, the upper opening of the central chamber is configured to be stepped.

[0011] Preferably, the support plate is smaller in height than the outer shell.

[0012] Preferably, the inner wall of the support plate is provided with a plurality of slots, the first baffle is slidably installed in the slots, and the support plate has various shapes.

[0013] Preferably, a sealing strip is provided at the bottom of the housing, and the sealing strip is located on both sides of the lower end of the first baffle.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] (1) The present invention realizes the co-culture of different types of cells with another specific cell by providing multiple upper chambers. Compared with the existing one-to-one culture method, the device can complete the co-culture of different types of cells with specific cells using only one culture chamber, which is convenient to operate;

[0016] (2) The present invention enriches the types of research applicable to co-cultured cells by providing an additional chamber, thereby improving the practicality of the device;

[0017] (3) The utility model sets the height of the support plate lower to facilitate the immersion of the diaphragm at the lower end of the fixed ring into the culture medium in the support plate, thereby preventing excessive culture medium in the support plate from overflowing when the chamber is covered. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic structural diagram of the first embodiment of the multi-cell co-culture device provided by the present invention;

[0019] Figure 2 This is an exploded view of the first embodiment of the multi-cell co-culture device provided by the present invention;

[0020] Figure 3 This is an exploded view of the second embodiment of the multi-cell co-culture device provided by the present invention;

[0021] Figure 4 This is a structural schematic diagram of the fifth embodiment of the multi-cell co-culture device provided by the present invention.

[0022] Among them, the names corresponding to the figure marks are: 101, outer shell; 102, support plate; 103, fixing ring; 104, upper chamber; 105, diaphragm; 106, central chamber; 107, additional chamber; 108, first baffle; 109, second baffle; 110, sealing strip; 111, slot. DETAILED DESCRIPTION

[0023] The present invention will be further described below with reference to the accompanying drawings and embodiments. The present invention includes but is not limited to the following embodiments.

[0024] First embodiment:

[0025] like Figure 1-2 As shown, the multi-cell co-culture device provided by the present invention includes: a shell 101, the shell 101 is the outermost shell of the culture chamber, a support plate 102 is fixedly installed at the bottom of the shell 101, the support plate 102 is annular, and the shell 101 is also the center of the circle, and the two are concentric circles. A first baffle 108 is provided in the support plate 102, and the first baffle 108 divides the area surrounded by the support plate 102 and the bottom of the shell 101 into several parts. The upper end of the support plate 102 is detachably provided with a plurality of upper chambers 104, which are used to hold one of the culture solutions and the planted cells, and different upper chambers 104 respectively hold different types of culture solutions and planted cells. All upper chambers 104 are mounted on a fixed ring 103. A diaphragm 105 is provided at the bottom of the upper chamber 104. The diaphragm 105 is a polycarbonate membrane, which can prevent the culture fluid in different culture chambers from flowing into each other, but allows cell metabolic products to pass through the membrane and has permeability. The edge of the fixed ring 103 can slide between the support plate 102 and the outer shell 101. When in use, first add a culture fluid to the space within the support plate 102 and plant cells, then put culture fluid into multiple upper chambers 104 one by one and inoculate different types of cells, and partially cover the upper end of the outer shell 101. Each separated space of the support plate 102 corresponds to an upper chamber 104.

[0026] By setting up multiple upper chambers 104, different types of cells and another specific cell can be co-cultured. Compared with the existing one-to-one culture method, this device can complete the co-culture of different types of cells and specific cells with one culture chamber, which is convenient to operate.

[0027] Second embodiment:

[0028] like Figure 3 As shown, in order to facilitate the study of the effects of co-culturing two or more cells on cells, a central chamber 106 is further provided at the bottom of the housing 101, and an additional chamber 107 is provided at the upper end of the central chamber 106. A second baffle 109 is provided in the additional chamber 107, and the second baffle 109 divides the internal space of the additional chamber 107 into two parts. A culture medium is added to the central chamber 106 and cells are planted. Another different culture medium is added to the additional chamber 107 and different types of cells are planted. The additional chamber 107 is then processed to cover the central chamber 106. Note that the culture media in different chambers must have overlapping parts. Finally, the two or more cell metabolites in the additional chamber 107 enter the culture media in the central chamber 106.

[0029] The additional chamber 107 is provided to enrich the types of research applicable to co-cultured cells and improve the practicability of the device.

[0030] Third embodiment:

[0031] like Figure 3 As shown, the upper opening of the central chamber 106 is set to be stepped. The stepped opening of the central chamber 106 is a base for placing the additional chamber 107. The additional chamber 107 has various specifications, corresponding to the steps at different positions on the opening of the central chamber 106.

[0032] The upper opening of the central chamber 106 is configured to be stepped to accommodate more types of additional chambers 107 , thereby improving the applicability of the device.

[0033] Fourth embodiment:

[0034] like Figure 2 As shown, the height of the support plate 102 is smaller than that of the housing 101 , and the upper end of the support plate 102 supports the fixing ring 103 , that is, the horizontal height of the lower end surface of the fixing ring 103 is related to the height of the support plate 102 .

[0035] By setting the height of the support plate 102 lower, the diaphragm 105 at the lower end of the fixing ring 103 is conveniently immersed in the culture solution in the support plate 102, thereby preventing the culture solution from overflowing when the chamber 104 is covered due to excessive placement of the culture solution in the support plate 102.

[0036] Fifth embodiment:

[0037] like Figure 4 As shown, the inner wall of the support plate 102 is provided with a plurality of slots 111, and the first baffle 108 is slidably installed in the slots 111. The support plate 102 has a variety of shapes. The different shapes of the support plate 102 correspond to different numbers of internal spaces divided by the support plate 102. A sealing strip 110 is provided at the bottom of the outer shell 101. The sealing strip 110 is located on both sides of the lower end of the first baffle 108. The sealing strip 110 can prevent the culture solution in different areas separated by the support plate 102 and the substances in the culture solution from being mixed together, resulting in inaccurate experimental results.

[0038] Working principle: First, a culture medium is added to the space within the support plate 102 and cells are planted. Then, the culture medium is placed in multiple upper chambers 104 one by one and different types of cells are inoculated. The upper chambers 104 are partially covered on the upper end of the outer shell 101. Each separated space of the support plate 102 corresponds to an upper chamber 104.

[0039] The above embodiment is only one of the preferred implementation methods of the present invention and should not be used to limit the scope of protection of the present invention. Any changes or modifications that have no substantive meaning made to the main design concept and spirit of the present invention, as long as the technical problems they solve are still consistent with the present invention, should be included in the scope of protection of the present invention.

Claims

1. A multi-cell co-culture device, characterized in that: include: A housing (101) is provided, wherein a support plate (102) is fixedly mounted on the bottom of the housing (101), the support plate (102) is annular, a first baffle (108) is provided inside the support plate (102), a plurality of upper chambers (104) are detachably provided on the upper end of the support plate (102), the upper chambers (104) are mounted on a fixing ring (103), a diaphragm (105) is provided at the bottom of the upper chambers (104), and an edge of the fixing ring (103) can slide between the support plate (102) and the housing (101).

2. The multi-cell co-culture device according to claim 1, characterized in that The bottom of the housing (101) is further provided with a central chamber (106), the upper end of the central chamber (106) is provided with an additional chamber (107), a second baffle (109) is provided in the additional chamber (107), and the second baffle (109) divides the internal space of the additional chamber (107) into two parts.

3. The multi-cell co-culture device according to claim 2, characterized in that: The upper opening of the central chamber (106) is configured in a stepped shape.

4. The multi-cell co-culture device according to claim 1, characterized in that The support plate (102) is smaller in height than the housing (101).

5. The multi-cell co-culture device according to claim 1, characterized in that: The inner wall of the support plate (102) is provided with a plurality of slots (111), the first baffle (108) is slidably installed in the slots (111), and the support plate (102) has various shapes.

6. The multi-cell co-culture device according to claim 5, characterized in that: A sealing strip (110) is provided at the bottom of the housing (101), and the sealing strip (110) is located on both sides of the lower end of the first baffle (108).

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