Microfluidic chip for LAMP (loop-mediated isothermal amplification) detection

Abstract

The utility model relates to the technical field of biomedical engineering, in particular to a micro-fluidic chip for LAMP (loop-mediated isothermal amplification) detection, which comprises a bottom plate and a cover plate, a sample storage cavity and a plurality of reaction cavities are formed in the bottom plate and are communicated through a pipeline, the transverse section of the bottom plate is of a circular structure, the pipeline comprises an inner flow pipe and an outer flow pipe, the inner flow pipe and the outer flow pipe are both communicated with the sample storage cavity and the reaction cavities, and the transverse section of the cover plate and the transverse section of the bottom plate are the same in shape; the cover plate is provided with a sample inlet corresponding to the sample storage cavity, the sample inlet is provided with a drainage port, the drainage port is provided with a protective film, and the cover plate is provided with a plurality of observation plates corresponding to the plurality of reaction cavities; according to the design of the microfluidic chip for LAMP detection, through the innovative design of the circular structure, the double-flow pipe system, the horn mouth and the protective film, the sample treatment efficiency and accuracy are remarkably improved, and meanwhile stable operation and long service life of the chip are ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedical engineering, and in particular to a microfluidic chip for LAMP detection. Background Art

[0002] Microfluidic chip technology, which precisely controls and manipulates fluids at a tiny scale, allows for the miniaturization of chemical, biological, or medical experiments. This technology is becoming increasingly important in scientific research and medical diagnostics due to its ability to achieve high-throughput screening, low reagent consumption, rapid reaction times, and automated operation. Microfluidic chips typically contain multiple micron- to millimeter-scale channels, reaction chambers, and other functional structures for performing operations such as mixing, separation, heating, and detection. However, existing microfluidic chip designs have several limitations, particularly regarding the introduction and processing of sample solutions. Traditional microfluidic chip designs typically consist of only a single inlet and a single flow channel, through which the sample solution flows into the reaction chamber. This single flow channel limits the flow rate of the sample solution into the reaction chamber, prolonging the entire detection process, especially when processing large numbers of samples. For applications requiring rapid response, such as rapid on-site diagnostic tests, a single flow channel design cannot meet the requirements for rapid sample introduction and processing, limiting the application of microfluidic chips in emergency situations. The inlet is a critical component of the microfluidic chip, directly interacting with the external environment. Without proper protection, the inlet may become contaminated or damaged, affecting chip performance and reliability. Utility Model Content

[0003] In view of this, the present invention addresses the deficiencies of the prior art and proposes a microfluidic chip for LAMP detection, aiming to solve at least one of the problems raised in the above background technology.

[0004] The utility model provides a microfluidic chip for LAMP detection, comprising: a bottom plate, on which a sample storage cavity and a plurality of reaction cavities are provided, wherein the sample storage cavity and the plurality of reaction cavities are connected via pipes, wherein the bottom plate has a circular cross-section, and the pipes include an inner flow tube and an outer flow tube, wherein both the inner flow tube and the outer flow tube are connected to the sample storage cavity and the reaction cavity;

[0005] A cover plate, wherein the transverse cross-section of the cover plate is the same as the transverse cross-section of the bottom plate, the cover plate is provided with an inlet corresponding to the sample storage cavity, the inlet is provided with a drainage port, the drainage port is provided with a protective film, and the cover plate is provided with a plurality of observation plates corresponding to the plurality of reaction chambers.

[0006] In some embodiments, the sample storage cavity is arranged at the center point of the film.

[0007] In some embodiments, a plurality of reaction chambers are arranged on the bottom film in a circular array with the sample storage chamber as the center point.

[0008] In some embodiments, the bottom surface of the cover sheet is fixedly connected to the top surface of the bottom sheet.

[0009] In some embodiments, the inner flow tube comprises:

[0010] a first inner flow tube, wherein a plurality of the first inner flow tubes are provided corresponding to the plurality of the reaction chambers, one end of the plurality of the first inner flow tubes is communicated with the sample storage chamber, and the other end of the plurality of the first inner flow tubes is communicated with the plurality of the reaction chambers respectively;

[0011] The second inner flow tube is provided in plurality and is used to be provided between two adjacent reaction chambers, with both ends of the second inner flow tube being respectively connected to corresponding sides of the two adjacent reaction chambers.

[0012] In some embodiments, the first inner flow tube coincides with the axis of the reaction chamber.

[0013] In some embodiments, both ends of the second inner flow tube are respectively connected to the center points of two adjacent side walls of the reaction chamber.

[0014] In some embodiments, the outflow tube comprises:

[0015] a first outflow tube, wherein a plurality of the first outflow tubes are provided corresponding to the plurality of reaction chambers, and one end of each of the plurality of first outflow tubes is connected to an end of the plurality of reaction chambers away from the sample storage chamber;

[0016] The second outflow tube has a 12-sided structure and is sleeved outside a plurality of reaction chambers. The other ends of a plurality of the first outflow tubes are connected to the second outflow tube. The second outflow tube is connected to the sample storage chamber through a third outflow tube having the same number as the plurality of the reaction chambers.

[0017] In some embodiments, the observation plate has the same shape as the reaction chamber.

[0018] In some embodiments, the drainage port is a bell-shaped port, the opening end of the bell-shaped port is upwardly arranged, the bottom of the bell-shaped port is fixedly connected to the injection port, and the top of the bell-shaped port is affixed with a protective film.

[0019] The foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the present disclosure.

[0020] Other features and aspects of the present disclosure will become more apparent from the following detailed description of exemplary embodiments with reference to the attached drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0022] Figure 1 A top view of the bottom sheet of the microfluidic chip for LAMP detection provided by an embodiment of the present utility model;

[0023] Figure 2 A top view of a cover sheet of a microfluidic chip for LAMP detection provided in an embodiment of the present invention;

[0024] Figure 3 This is a front view of the microfluidic chip for LAMP detection provided by an embodiment of the present utility model.

[0025] Among them: 1. Film; 2. Sample storage chamber; 3. Reaction chamber; 4. Inner flow tube; 5. Outer flow tube; 6. Cover plate; 7. Inlet; 8. Protective film; 9. Observation plate; 10. First inner flow tube; 11. Second inner flow tube; 12. First outer flow tube; 13. Second outer flow tube; 14. Third outer flow tube; 15. Bell mouth. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0027] In the description of this application, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application.

[0028] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature specified as "first" or "second" may explicitly or implicitly include one or more of such features. Throughout this application, unless otherwise specified, "plurality" means two or more.

[0029] In the description of this application, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed, detachable, or integral connections; mechanical or electrical connections; direct or indirect connections through an intermediate medium; and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this application based on the specific circumstances.

[0030] As mentioned in the background technology, traditional microfluidic chip designs usually only include one injection port and one flow channel. The sample solution flows into the reaction chamber through this single channel. Since there is only one flow channel, the speed at which the sample solution flows into the reaction chamber is limited, which prolongs the time of the entire detection process. Especially when a large number of samples need to be processed, for application scenarios that require rapid response, such as on-site rapid diagnostic tests, the single flow channel design cannot meet the needs of rapid injection and processing, thereby limiting the application of microfluidic chips in emergency situations. The injection port is a key part of the microfluidic chip, which is in direct contact with the external environment. Without proper protection measures, the injection port may be contaminated or damaged, affecting the performance and reliability of the chip.

[0031] To improve the above problems, the present application proposes a microfluidic chip for LAMP detection, which adopts a circular structure chip design. Compared with traditional linear or square flow channels, it reduces the resistance and eddy current phenomenon of the fluid during the flow process, thereby accelerating the flow rate of the sample solution; the simultaneous use of internal and external flow tubes realizes rapid distribution and mixing of samples, further improving the detection efficiency; the design of a bell-shaped shape at the injection port can effectively guide the fluid to smoothly enter the interior of the chip, and minimize the risk of overflow even during rapid injection or operation using automated equipment; the protective film added at the bell-shaped port can not only prevent contaminants from entering the flow channel, but also avoid accidental damage to the injection port during operation, thereby maintaining the stability and reliability of the chip.

[0032] See Figure 1-3 As shown, a microfluidic chip for LAMP detection according to an embodiment of the present application includes:

[0033] A film 1 is provided with a sample storage chamber 2 and a plurality of reaction chambers 3. The sample storage chamber 2 and the plurality of reaction chambers 3 are connected by pipes. The transverse cross-section of the film 1 is a circular structure. The pipes include an inner flow pipe 4 and an outer flow pipe 5. The inner flow pipe 4 and the outer flow pipe 5 are both connected to the sample storage chamber 2 and the reaction chamber 3.

[0034] The cover plate 6 has a transverse cross-section having the same shape as that of the bottom plate 1 , and a sample inlet 7 is provided on the cover plate 6 corresponding to the sample storage cavity 2 , a drainage port is provided at the sample inlet 7 , and a protective film 8 is provided at the drainage port, and several observation plates 9 are provided on the cover plate 6 corresponding to the several reaction cavities 3 .

[0035] In some specific embodiments, the sample storage chamber 2 is disposed at the center of the bottom film 1 .

[0036] In some specific embodiments, a plurality of reaction chambers 3 are arranged on the bottom film 1 in a circular array with the sample storage chamber 2 as the center point.

[0037] In some specific embodiments, the bottom surface of the cover sheet 6 is fixedly connected to the top surface of the bottom sheet 1 .

[0038] In some specific embodiments, the inner flow tube 4 includes:

[0039] A first inner flow tube 10 is provided corresponding to a plurality of reaction chambers 3. One end of each of the first inner flow tubes 10 is connected to the sample storage chamber 2, and the other end of each of the first inner flow tubes 4 is connected to the plurality of reaction chambers 3;

[0040] The second inner flow tube 11 is provided in plurality and is used to be provided between two adjacent reaction chambers 3 , with both ends of the second inner flow tube 11 being connected to corresponding sides of the two adjacent reaction chambers 3 .

[0041] In some specific embodiments, the first inner flow tube 10 coincides with the axis of the reaction chamber 3 .

[0042] In some specific embodiments, both ends of the second inner flow tube 11 are respectively connected to the center points of the side walls of two adjacent reaction chambers 3 .

[0043] In some specific embodiments, the outflow tube 5 includes:

[0044] A first outflow tube 12, wherein a plurality of first outflow tubes 12 are provided corresponding to the plurality of reaction chambers 3, and one end of each of the plurality of first outflow tubes 12 is connected to an end of the plurality of reaction chambers 3 away from the sample storage chamber 2;

[0045] The second outflow tube 13 has a twelve-sided structure. The second outflow tube 13 is sleeved outside the reaction chambers 3. The other ends of the multiple first outflow tubes 12 are connected to the second outflow tube 13. The second outflow tube 13 is connected to the sample storage chamber 2 through the third outflow tubes 14, the same number as the reaction chambers 3.

[0046] In some specific embodiments, the observation plate 9 has the same shape as the reaction chamber 3 .

[0047] In some specific embodiments, the drainage port is a bell mouth 15 , the opening end of the bell mouth 15 is set upward, the bottom of the bell mouth 15 is fixedly connected to the injection port 7 , and the top of the bell mouth 15 is attached with a protective film 8 .

[0048] It should be understood that the upward design of the opening end of the bell mouth 15 is conducive to utilizing gravity to help the fluid flow in naturally, reducing the need for additional power. By optimizing the shape and angle of the bell mouth 15, the effect of reducing fluid turbulence and eddy currents can be achieved, thereby reducing energy loss.

[0049] Obviously, those skilled in the art may make various modifications and variations to the present invention without departing from the spirit and scope of the present invention. Thus, if such modifications and variations fall within the scope of the claims of the present invention and their equivalents, the present invention is intended to include such modifications and variations.

Claims

1. A microfluidic chip for LAMP detection, characterized in that: include: A bottom plate, wherein the bottom plate is provided with a sample storage cavity and a plurality of reaction cavities, wherein the sample storage cavity and the plurality of reaction cavities are connected via pipes, wherein the bottom plate has a circular cross-section, and the pipes include an inner flow pipe and an outer flow pipe, wherein both the inner flow pipe and the outer flow pipe are connected to the sample storage cavity and the reaction cavities; A cover plate, wherein the transverse cross-section of the cover plate is the same as the transverse cross-section of the bottom plate, the cover plate is provided with an inlet corresponding to the sample storage cavity, the inlet is provided with a drainage port, the drainage port is provided with a protective film, and the cover plate is provided with a plurality of observation plates corresponding to the plurality of reaction chambers.

2. A microfluidic chip for LAMP detection according to claim 1, characterized in that: The sample storage cavity is arranged at the center point of the film.

3. A microfluidic chip for LAMP detection according to claim 2, characterized in that: A plurality of reaction chambers are arranged on the bottom film in a circular array with the sample storage chamber as the center point.

4. A microfluidic chip for LAMP detection according to claim 3, characterized in that: The bottom surface of the cover sheet is fixedly connected to the top surface of the bottom sheet.

5. A microfluidic chip for LAMP detection according to claim 3, characterized in that: The inner flow tube comprises: a first inner flow tube, wherein a plurality of the first inner flow tubes are provided corresponding to the plurality of the reaction chambers, one end of the plurality of the first inner flow tubes is communicated with the sample storage chamber, and the other end of the plurality of the first inner flow tubes is communicated with the plurality of the reaction chambers respectively; The second inner flow tube is provided in plurality and is used to be provided between two adjacent reaction chambers, with both ends of the second inner flow tube being respectively connected to corresponding sides of the two adjacent reaction chambers.

6. A microfluidic chip for LAMP detection according to claim 5, characterized in that: The first inner flow tube coincides with the axis of the reaction chamber.

7. A microfluidic chip for LAMP detection according to claim 6, characterized in that: Two ends of the second inner flow tube are respectively connected to the center points of two adjacent reaction chamber side walls.

8. The microfluidic chip for LAMP detection according to claim 5, characterized in that: The outflow pipe comprises: a first outflow tube, wherein a plurality of the first outflow tubes are provided corresponding to the plurality of reaction chambers, and one end of each of the plurality of first outflow tubes is connected to an end of the plurality of reaction chambers away from the sample storage chamber; The second outflow tube has a 12-sided structure and is sleeved outside a plurality of reaction chambers. The other ends of a plurality of the first outflow tubes are connected to the second outflow tube. The second outflow tube is connected to the sample storage chamber through a third outflow tube having the same number as the plurality of the reaction chambers.

9. A microfluidic chip for LAMP detection according to claim 1, characterized in that: The observation plate has the same shape as the reaction chamber.

10. The microfluidic chip for LAMP detection according to claim 1, characterized in that: The drainage port is a bell-shaped port, the opening end of the bell-shaped port is upwardly arranged, the bottom of the bell-shaped port is fixedly connected to the injection port, and the top of the bell-shaped port is affixed with a protective film.

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