Efficient phage separation device for phage culture

Abstract

The utility model relates to the technical field of bacteriophage culture, and discloses an efficient bacteriophage separation device for bacteriophage culture, which comprises a separation tank, a plurality of filter plates with different filter apertures are detachably arranged in the separation tank, stirring blades are rotatably arranged above the filter plates in the separation tank, and the stirring blades are driven by a motor fixedly arranged on the upper wall of the separation tank. A feeding pipe and a pressure valve are arranged on the upper wall of the separation tank, a heater and a refrigerator which are used for regulating and controlling the temperature in the separation tank are connected to the two sides of the separation tank respectively, a multi-directional washing frame is arranged in the separation tank, and a washing mechanism connected with the multi-directional washing frame is connected to the outer side of the separation tank. The bacteriophage separation tank has the advantages that the bacteriophage filtration separation effect is good, and the temperature in the separation tank is convenient to adjust.

Description

Technical Field

[0001] The utility model relates to the technical field of bacteriophage culture, in particular to a bacteriophage high-efficiency separation device for bacteriophage culture. Background Art

[0002] Bacteriophages are viruses that can only replicate and proliferate within living microbial cells. They are tiny and have diverse morphologies, primarily tadpole-shaped, microsphere-shaped, and thin rod-shaped. Phages are widely distributed and primarily infect bacteria and other microorganisms. Their life cycle consists of five stages: adsorption, invasion, biosynthesis, assembly, and release. Phages are categorized as either virulent or temperate. They can be used for detecting and identifying bacteria, treating bacterial infections, as vectors for genetic engineering, in food preservation, and as sterilizers in aquaculture and crop production. However, the specificity of phages also imposes some application limitations.

[0003] Currently available phage separation devices have certain technical limitations. Most devices mainly use single-layer filtration to separate phages from impurities in the culture medium. However, this method often leads to insufficient filtration. Due to the limited accuracy of single-layer filtration, it is difficult to completely separate phages from impurities, which is extremely detrimental to the final sample output. In addition, there are also difficulties in controlling the temperature and humidity inside the separation tank. Temperature and humidity that are difficult to accurately control will greatly affect the phage separation effect. Utility Model Content

[0004] In order to solve the above-mentioned problems, the utility model proposes a high-efficiency phage separation device for phage culture, which has good filtering and separation effect on phages and is convenient for adjusting the temperature in the separation tank.

[0005] In order to solve the above-mentioned technical problems, the technical solution proposed in the utility model is: a high-efficiency phage separation device for phage culture, comprising a separation tank, wherein a plurality of filter plates with different filter apertures are detachably provided in the separation tank, a stirring blade is rotatably provided above the filter plate in the separation tank, and the stirring blade is driven by a motor fixed on the upper wall of the separation tank, a feed pipe and a pressure valve are provided on the upper wall of the separation tank, a heater and a refrigerator for regulating the temperature in the separation tank are respectively connected on both sides of the separation tank, a multi-directional flushing rack is provided in the separation tank, and a flushing mechanism connected to the multi-directional flushing rack is connected to the outside of the separation tank.

[0006] Furthermore, the filter plates are arranged up and down, and the filter holes on the filter plates decrease in size from top to bottom.

[0007] Based on the above characteristics, the filtration is made more refined and hierarchical, which greatly improves the separation purity of the bacteriophage and ensures that the bacteriophage can be separated efficiently.

[0008] Furthermore, the filter plate is clamped in the separation tank, and the upper wall of the separation tank is detachable.

[0009] Based on the above features, it is convenient to install and remove the filter plate. When the filter plate needs to be replaced or cleaned, the operation can be carried out easily, which improves the maintenance efficiency of the equipment.

[0010] Furthermore, two stirring blades are symmetrically provided, each stirring blade is a wavy plate, and a rotating shaft is connected to the end of the stirring blade, and the motor drives the stirring blade to rotate through the rotating shaft.

[0011] Based on the above characteristics, the wavy stirring blade can increase the contact area between the stirring blade and the substance to be separated, so that the bacteriophage to be separated can be more fully mixed and flowed in the separation tank, avoiding local precipitation or uneven distribution, thereby improving the filtration separation effect.

[0012] Furthermore, a plurality of flushing nozzles facing in different directions are provided below the multi-directional flushing rack.

[0013] Based on the above characteristics, the impurities and bacteriophages on the filter plate can be washed in all directions, so that the bacteriophages and impurities can be separated efficiently and thoroughly.

[0014] Furthermore, the flushing mechanism includes a water tank fixed on the side wall of the separation tank, the water tank is connected to a water pipe connected to the multi-directional flushing rack, the lower end of the water pipe extends into the bottom end of the water tank, and the water pipe is connected to a water pump.

[0015] The aforementioned features provide a stable water source for the multi-directional flushing rack. The water tank can store sufficient water to meet multiple flushing needs. The lower end of the water pipe extends into the bottom of the tank, ensuring the water pump can pump water to the bottom of the tank, ensuring a stable supply of flushing water. The pump provides sufficient pressure, allowing flushing water to be ejected from the flushing nozzle at a high velocity, enhancing the flushing effect and ensuring thorough separation of bacteriophages from impurities.

[0016] Compared with the prior art, the advantages of the present invention are as follows: a plurality of filter plates with different filter apertures can be detachably provided in the separation tank, which can gradually perform fine filtration on the bacteriophages, and impurities of different sizes can be intercepted at different levels of the filter plates, ensuring that the bacteriophages are fully separated and purified; the rotation of the stirring blade driven by the motor can make the substances to be separated more evenly distributed in the separation tank, avoiding local accumulation, thereby improving the efficiency and effect of filtration; the multi-directional flushing rack and the impurities on the filter plate after the phages are separated are flushed, preventing the bacteriophages from adhering to the impurities and remaining on the filter plate, thereby ensuring that the bacteriophages are fully separated and recovered.

[0017] The heater and refrigerator can quickly adjust the temperature in the separation tank to the required range according to the different needs of phage culture, meet the temperature sensitivity requirements of phage, and help improve the separation efficiency and quality of phage, while also providing more suitable environmental conditions for phage culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a three-dimensional diagram of the utility model;

[0019] Figure 2 It is the main view of the utility model;

[0020] Figure 3 It is a top view of the utility model;

[0021] Figure 4 It is a cross-sectional view of the present utility model.

[0022] As shown in the figure: 1. Separation tank; 2. Filter plate; 3. Stirring blade; 4. Motor; 5. Feed pipe; 6. Pressure valve; 7. Heater; 8. Refrigerator; 9. Multi-directional flushing rack; 10. Rotating shaft; 11. Flushing nozzle; 12. Water tank; 13. Water pipe; 14. Water pump. DETAILED DESCRIPTION

[0023] The present invention will be described in further detail below with reference to the accompanying drawings.

[0024] Combined with attachment Figure 4 A highly efficient phage separation device for phage culture comprises a separation tank 1, within which are removably provided several filter plates 2 of varying pore sizes. The filter plates 2 are arranged vertically, with the pores decreasing from top to bottom, resulting in a more refined and hierarchical filtration process. This significantly improves the purity of phage separation and ensures efficient phage separation. The filter plates 2 are snap-fitted into the separation tank 1, and the upper wall of the separation tank 1 is removable, facilitating installation and removal of the filter plates 2. Replacement or cleaning of the filter plates 2 is easily accomplished, improving equipment maintenance efficiency.

[0025] Combined with attachment Figure 4 In the separation tank 1, a stirring blade 3 is rotatably provided above the filter plate 2. The stirring blade 3 is driven by a motor 4 fixed to the upper wall of the separation tank 1. Two stirring blades 3 are symmetrically provided. The stirring blade 3 is a wavy plate. The end of the stirring blade 3 is connected with a rotating shaft 10. The motor 4 drives the stirring blade 3 to rotate through the rotating shaft 10. The wavy stirring blade 3 can increase the contact area between the stirring blade 3 and the substance to be separated, so that the phage to be separated can be more fully mixed and flowed in the separation tank 1, avoiding local precipitation or uneven distribution, thereby improving the effect of filtration separation.

[0026] Combined with attachment Figure 3 , Attachment Figure 4 A feed pipe 5 and a pressure valve 6 are provided on the upper wall of the separation tank 1. A heater 7 and a refrigerator 8 for regulating the temperature inside the separation tank 1 are respectively connected to both sides of the separation tank 1. A multi-directional flushing rack 9 is provided inside the separation tank 1. A plurality of flushing nozzles 11 facing in different directions are provided below the multi-directional flushing rack 9, which can perform all-round flushing of impurities and bacteriophages on the filter plate 2, so that the bacteriophages and impurities can be efficiently and thoroughly separated.

[0027] Combined with attachment Figure 1 , Attachment Figure 2 , a flushing mechanism connected to the multi-directional flushing rack 9 is connected to the outer side of the separation tank 1. The flushing mechanism includes a water tank 12 fixed on the side wall of the separation tank 1, and a water pipe 13 connected to the multi-directional flushing rack 9 is connected to the water tank 12. The lower end of the water pipe 13 is inserted into the bottom end of the water tank 12, and a water pump 14 is connected to the water pipe 13, which provides a stable flushing water source for the multi-directional flushing rack 9. The water tank 12 can store enough water to meet the needs of multiple flushing. The lower end of the water pipe 13 is inserted into the bottom end of the water tank 12, which can ensure that the water pump 14 can draw water to the bottom of the water tank 12, ensuring a stable supply of flushing water. The setting of the water pump 14 can provide sufficient pressure so that the flushing water can be sprayed out from the flushing nozzle 11 at a higher speed, thereby enhancing the flushing effect and ensuring that the bacteriophage is completely separated from the impurities.

[0028] The specific implementation of the utility model is as follows: the device is connected to an external power supply, and the phage culture liquid to be separated is added into the separation tank 1 through the feed pipe 5. The phage culture liquid falls on the filter plate 2. As it continues to flow downward, each filter plate 2 filters it step by step, and the impurities therein remain on each filter plate 2. At this time, some phages are still attached to the impurities and remain on the filter plate 2. The water pump 14 is turned on, and the water pipe 13 draws water or a specific pure culture liquid in the water tank 12 and sprays it onto the filter plate 2 through the flushing nozzle 11. The water or pure culture liquid is left on the impurities. The impurities on the filter plate 2 are rinsed, and at the same time, the motor 4 is turned on. The motor 4 drives the stirring blade 3 to rotate through the rotating shaft 10. The stirring blade 3 stirs the water or pure culture solution to separate the residual bacteriophage from the impurities and flow down with the water. Finally, the separated bacteriophage flows out through the discharge port at the lower end of the separation tank 1 and is collected and recycled. During the process, the heater 7 or the refrigerator 8 can be turned on to adjust the temperature in the separation tank 1 to meet the culture needs of the bacteriophage. Finally, the upper cover of the separation tank 1 is removed, and the filter plate 2 is taken out for cleaning to avoid clogging and affecting the subsequent separation effect.

[0029] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the term "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection. For ordinary technicians in this field, the specific meaning of the above terms in the present invention can be understood according to specific circumstances.

[0030] The above description of the present invention and its embodiments is non-limiting. The drawings show only one embodiment of the present invention, and the actual structure is not limited thereto. In short, if a person skilled in the art is inspired by the above, and does not deviate from the purpose of the present invention, without inventive design, a structure and embodiment similar to the technical solution should fall within the scope of protection of the present invention.

Claims

1. A phage efficient separation device for phage culture, comprising a separation tank (1), characterized in that: The separation tank (1) is provided with a plurality of filter plates (2) with different filter apertures that can be removed. A stirring blade (3) is rotatably provided above the filter plate (2) in the separation tank (1). The stirring blade (3) is driven by a motor (4) fixed on the upper wall of the separation tank (1). A feed pipe (5) and a pressure valve (6) are provided on the upper wall of the separation tank (1). A heater (7) and a refrigerator (8) for regulating the temperature in the separation tank (1) are respectively connected on both sides of the separation tank (1). A multi-directional flushing rack (9) is provided in the separation tank (1), and a flushing mechanism connected to the multi-directional flushing rack (9) is connected to the outside of the separation tank (1).

2. The phage efficient separation device for phage culture according to claim 1, characterized in that: The filter plates (2) are arranged up and down, and the filter holes on the filter plates (2) decrease in size from top to bottom.

3. The phage efficient separation device for phage culture according to claim 1, characterized in that: The filter plate (2) is clamped in the separation tank (1), and the upper wall of the separation tank (1) is detachable.

4. The phage efficient separation device for phage culture according to claim 1, characterized in that: The stirring blades (3) are symmetrically provided with two of them. The stirring blades (3) are wavy plates. The ends of the stirring blades (3) are connected with a rotating shaft (10). The motor (4) drives the stirring blades (3) to rotate via the rotating shaft (10).

5. The phage efficient separation device for phage culture according to claim 1, characterized in that: A plurality of flushing nozzles (11) facing in different directions are provided below the multi-directional flushing frame (9).

6. The phage efficient separation device for phage culture according to claim 1, characterized in that: The flushing mechanism comprises a water tank (12) fixedly arranged on the side wall of the separation tank (1); a water pipe (13) communicating with the multi-directional flushing rack (9) is connected to the water tank (12); the lower end of the water pipe (13) extends into the bottom end of the water tank (12); and a water pump (14) is connected to the water pipe (13).

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