Anaerobic culture device capable of detecting whether cover of culture tank is tightened or not

Abstract

The utility model discloses an anaerobic culture device capable of detecting whether a cover of a culture tank is tightened, which belongs to the field of microbial culture and comprises a box body, a mounting seat fixedly mounted at the top of the box body, a culture tank mounted at the outer end of the bottom of the mounting seat in a threaded manner, and a connecting column fixedly mounted in the center of the mounting seat. An indicator vessel is fixedly installed at the bottom of the connecting column and installed in the culture tank, a methylene blue indicator is arranged in the indicator vessel, a gas conveying pipe, a gas suction pipe and a pressure sensor are installed on the circumferential portion of the installation base, and the methylene blue indicator detects whether oxygen in the culture tank is consumed or not; indicating that the culture tank reaches an anaerobic state, and detecting whether the cover is tightened or not; internal air can be pumped out through the exhaust pipe, other non-oxygen gas enters the culture tank through the gas conveying pipe, the pressure sensor monitors internal pressure, whether leakage occurs or not can be reflected by monitoring pressure changes, and therefore whether a cover of the culture tank is tightened or not can be detected.

Description

Technical Field

[0001] The utility model relates to the field of microbial culture, in particular to an anaerobic culture device capable of detecting whether a culture tank cover is tightened. Background Art

[0002] When culturing microorganisms, some require anaerobic culture, which is a process of isolating the microorganisms from molecular oxygen.

[0003] During anaerobic culture, the microbial strains need to be placed in a culture tank, and the microorganisms need to be placed in a pressure-resistant container for vacuum culture, or filled with carbon dioxide, hydrogen, nitrogen, argon, etc. to achieve an anaerobic state. However, the lid of the culture tank may not be tightened, so that the culture conditions cannot be met, resulting in culture failure. Utility Model Content

[0004] Purpose of the invention: The purpose of the utility model is to provide an anaerobic culture device with the function of detecting whether the culture tank lid is tightened.

[0005] Technical solution: It includes a box body, a mounting base is fixedly installed on the top of the box body, a culture tank is threadedly installed on the outer end of the bottom of the mounting base, a connecting column is fixedly installed at the center of the mounting base, an indicator dish is fixedly installed at the bottom of the connecting column, the indicator dish is installed inside the culture tank, the indicator dish contains methylene blue indicator, an air supply pipe, an exhaust pipe and a pressure sensor are installed on the circumference of the mounting base, the culture tank is made of glass material, a control panel is installed on the outside of the box body, and a control board is installed inside the control panel.

[0006] By adopting the above technical solution, there is a methylene blue indicator inside the indicator dish. The methylene blue indicator is used to determine whether the oxygen in the culture tank has been consumed. When the methylene blue turns colorless, it means that the culture tank has reached an anaerobic state. The exhaust pipe is capable of extracting the internal air, and the air supply pipe allows other non-oxygen gases to enter the culture tank. The pressure sensor monitors the internal pressure. By monitoring the pressure change, it can respond to whether there is a leak, thereby realizing the detection of whether the culture tank lid is tightened.

[0007] Optionally, a door is rotatably mounted on the front end of the box, a handle is fixedly mounted on the outer end of the door, the door is mounted by hinges, and the handle is fixedly mounted on the outer end of the door by bolts.

[0008] By adopting the above technical solution, the door is used to cover the box body, so that an independent space is formed inside the box body.

[0009] Optionally, a plurality of bases are fixedly installed on the bottom of the box, and the plurality of bases are fixedly installed on the bottom of the box by means of bolts.

[0010] By adopting the above technical solution, the base is used to support the box body so that the box body can be placed stably.

[0011] Optionally, a heating device is fixedly installed at the bottom of the box, a temperature sensor is installed on one side of the box, and a heating wire is installed inside the heating device.

[0012] By adopting the above technical solution, the heating device is used to increase the temperature inside the box and to cultivate the internal microorganisms.

[0013] Optionally, a lighting lamp is fixedly installed on the upper inner side of the box, and the lighting lamp is an LED lamp.

[0014] By adopting the above technical solution, the lighting lamp is used for lighting inside the box.

[0015] Optionally, a switch 1 is fixedly installed on the upper part of the gas transmission pipe, the switch 1 is installed with a connecting seat through a connecting pipe 1, a gas tank is installed on the top of the connecting seat, and the switch 1 is an electromagnetic switch.

[0016] By adopting the above technical solution, the switch is turned on and off to control the connection between the gas pipe and the top gas tank, so that the gas can enter the culture tank.

[0017] Optionally, a switch 2 is fixedly installed on the upper part of the exhaust pipe, and the switch 2 is installed with an air pump through a connecting pipe 2. The switch 2 is an electromagnetic switch.

[0018] By adopting the above technical solution, the switch is turned on and off to control the connection between the exhaust pipe and the top air pump, thereby achieving the discharge of gas inside the culture tank.

[0019] Optionally, an exhaust pipe is installed at the outer end of the air pump, and the exhaust pipe is installed by threading.

[0020] By adopting the above technical solution, the gas inside the culture tank is discharged from the exhaust pipe.

[0021] Beneficial effects: 1. The indicator dish in the present invention contains a methylene blue indicator, which detects whether the oxygen in the culture tank has been consumed. When the methylene blue turns colorless, it indicates that the culture tank has reached an oxygen-free state and detects whether the lid is tightened; 2. The exhaust pipe provided in the present invention can extract the internal air, and the air supply pipe allows other non-oxygen gases to enter the culture tank. The pressure sensor monitors the internal pressure. By monitoring the pressure change, it can respond to whether there is a leak, thereby realizing the detection of whether the culture tank lid is tightened. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is an overall structural diagram of an embodiment of the utility model;

[0023] Figure 2 It is an internal structure diagram of an embodiment of the utility model;

[0024] Figure 3This is a structural diagram of anaerobic detection according to an embodiment of the present utility model;

[0025] Figure 4 This is a diagram of the air supply structure of an embodiment of the utility model;

[0026] Figure 5 This is an exhaust structure diagram of an embodiment of the utility model;

[0027] Explanation of the accompanying symbols: 1. Box body; 2. Base; 3. Door; 4. Handle; 5. Connecting seat; 6. Air pump; 7. Heating device; 8. Temperature sensor; 9. Lighting lamp; 10. Mounting seat; 11. Culture tank; 12. Pressure sensor; 13. Connecting column; 14. Gas pipe; 15. Exhaust pipe; 16. Indicator dish; 17. Switch 1; 18. Connecting pipe 1; 19. Gas tank; 20. Switch 2; 21. Connecting pipe 2; 22. Exhaust pipe. DETAILED DESCRIPTION

[0028] In order to make the technical solution of the present invention clearer, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments. Figures 1 to 5 , including a box body 1, a mounting base 10 is fixedly installed on the top of the box body 1, a culture tank 11 is threadedly installed on the outer end of the bottom of the mounting base 10, a connecting column 13 is fixedly installed at the center of the mounting base 10, and an indicator dish 16 is fixedly installed at the bottom of the connecting column 13. The indicator dish 16 is installed inside the culture tank 11, and there is a methylene blue indicator inside the indicator dish 16. The circumferential part of the mounting base 10 is installed with an air supply pipe 14, an exhaust pipe 15 and a pressure sensor 12. The indicator dish 16 in the utility model has a methylene blue indicator inside. The methylene blue indicator detects whether the oxygen in the culture tank has been consumed. When the methylene blue becomes colorless, it indicates that the culture tank has reached an anaerobic state and detects whether the lid is tightened; 2 The exhaust pipe 15 set in the utility model can extract the internal air, and the air supply pipe 14 allows other non-oxygen gases to enter the interior of the culture tank 11. The pressure sensor 12 monitors the internal pressure. By monitoring the pressure change, it can respond to whether there is a leak, thereby realizing the detection of whether the culture tank lid is tightened.

[0029] A door 3 is rotatably mounted on the front end of the box body 1, and a handle 4 is fixedly mounted on the outer end of the door 3. The door 3 is used to cover the box body to form an independent space inside the box body, and the handle 4 is used to pull the door 3 to open and close it.

[0030] A plurality of bases 2 are fixedly mounted on the bottom of the box body 1 , and the bases 2 are used to support the box body 1 so that the box body 1 can be placed stably.

[0031] A heating device 7 is fixedly installed at the bottom of the box 1, and a temperature sensor 8 is installed on one side of the box 1. The heating device 7 is used to increase the temperature inside the box 1 for cultivating internal microorganisms.

[0032] A lighting lamp 9 is fixedly installed on the upper inner side of the box body 1, and the lighting lamp 9 is used for lighting the interior of the box body.

[0033] A switch 17 is fixedly installed on the upper part of the gas supply pipe 14. The switch 17 is installed with a connecting seat 5 through a connecting pipe 18. A gas tank 19 is installed on the top of the connecting seat 5. The switch 17 is on and off to control the connection between the gas supply pipe 14 and the top gas tank 19, so that the gas can enter the culture tank 11.

[0034] A switch 20 is fixedly installed on the upper part of the exhaust pipe 15. The switch 20 is installed with the air pump 6 through the connecting pipe 21. The switch 1 20 is turned on and off to control the connection between the exhaust pipe 15 and the top air pump 6, so as to discharge the gas inside the culture tank 11.

[0035] An exhaust pipe 22 is installed at the outer end of the air pump 6 , and the gas inside the culture tank 11 is discharged from the exhaust pipe 22 .

[0036] The embodiment of the present application is an anaerobic culture device with a function of detecting whether the culture tank cover is tightened or not. The implementation principle is as follows: when using the anaerobic culture device with a function of detecting whether the culture tank cover is tightened or not, the door 3 is opened, the anaerobic microorganisms to be cultured are placed in the culture tank 11, the methylene blue indicator is placed in the indicator dish 16, the culture tank 11 is screwed to the lower part of the mounting base 10, the control switch 20 is turned on, the air pump 6 is controlled to work, and the gas inside the culture tank 11 enters the connecting pipe 21 from the exhaust pipe 15, and then the gas inside the culture tank 11 enters the connecting pipe 21 from the exhaust pipe 15. The exhaust pipe 22 is used to discharge gas, thereby reducing the air inside the culture tank 11. After that, the control switch 17 is opened, and the oxygen-free gas inside the gas tank 19 enters the connecting pipe 18 and enters the culture tank 11 from the gas pipe 14, so that the culture tank 11 is filled with gas. After the residual gas is discharged, the switch 17 and the switch 2 20 are closed, and the pressure sensor 12 detects the internal pressure to determine whether there is a leak and whether the culture tank lid is tightened. The methylene blue indicator detects whether the oxygen in the culture tank has been consumed and whether the lid is tightened.

[0037] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be determined by the appended claims.

Claims

1. An anaerobic culture device capable of detecting whether the culture tank lid is tightened, characterized in that: The invention comprises a box body (1), a mounting seat (10) is fixedly mounted on the top of the box body (1), a culture tank (11) is threadedly mounted on the outer end of the bottom of the mounting seat (10), a connecting column (13) is fixedly mounted at the center of the mounting seat (10), an indicator dish (16) is fixedly mounted on the bottom of the connecting column (13), the indicator dish (16) is mounted inside the culture tank (11), and a methylene blue indicator is contained in the indicator dish (16), and an air supply pipe (14), an air extraction pipe (15) and a pressure sensor (12) are mounted on the circumference of the mounting seat (10).

2. The anaerobic culture device capable of detecting whether the culture tank lid is tightened according to claim 1, characterized in that: A door (3) is rotatably mounted on the front end of the box body (1), and a handle (4) is fixedly mounted on the outer end of the door (3).

3. The anaerobic culture device capable of detecting whether the culture tank lid is tightened according to claim 1, characterized in that: A plurality of bases (2) are fixedly mounted on the bottom of the box (1).

4. The anaerobic culture device capable of detecting whether the culture tank lid is tightened according to claim 1, characterized in that: A heating device (7) is fixedly installed at the bottom end of the box body (1), and a temperature sensor (8) is installed on one side of the box body (1).

5. The anaerobic culture device capable of detecting whether the culture tank lid is tightened according to claim 1, characterized in that: A lighting lamp (9) is fixedly mounted on the upper inner portion of the box body (1).

6. The anaerobic culture device capable of detecting whether the culture tank lid is tightened according to claim 1, characterized in that: A switch 1 (17) is fixedly installed on the upper portion of the gas delivery pipe (14), and the switch 1 (17) is installed with a connecting seat (5) through a connecting pipe 1 (18), and a gas tank (19) is installed on the top of the connecting seat (5).

7. The anaerobic culture device capable of detecting whether the culture tank lid is tightened according to claim 1, characterized in that: A second switch (20) is fixedly mounted on the upper portion of the air extraction pipe (15), and the second switch (20) is mounted with an air pump (6) via a second connecting pipe (21).

8. The anaerobic culture device capable of detecting whether the culture tank lid is tightened according to claim 7, characterized in that: An exhaust pipe (22) is installed at the outer end of the air pump (6).

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