Organ-like biological tissue culture device

Abstract

The utility model provides an organ-like biological tissue culture device, and relates to the technical field of biological tissue culture, the organ-like biological tissue culture device comprises a working table and a mounting table, a plurality of injection pipes fixedly penetrate through the top of the mounting table at equal intervals, the bottoms of the injection pipes are fixedly connected with injection heads, and the tops of the injection pipes are fixedly communicated with liquid extraction pipes. According to the utility model, the injection pipes, the injection heads, the flow regulating valves, the fixed displacement pumps and the liquid pumping pipes are matched for use, so that injection and adding work can be simultaneously carried out on a plurality of culture dishes, the injection efficiency can be improved, the flow velocity and flow of liquid are ensured, and accurate release and uniform distribution are ensured; and a rotating disc, a rotating shaft, a supporting rod, a first electric push rod, a connecting rod, a round block, an arc-shaped plate and a push plate are used in cooperation, the culture dishes added in the containing groove can be rapidly pushed out, the culture dishes can be conveniently taken out, and the injection adding efficiency of the culture dishes is further improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biological tissue culture, in particular to an organoid biological tissue culture device. Background Art

[0002] In the existing technology, tissue culture devices can only add culture fluid or sealing oil to one culture dish at a time, and then switch to the next culture dish to add culture fluid or sealing oil. It is impossible to add culture fluid or sealing oil to different culture dishes, which increases the operation time and reduces the efficiency of addition. For experiments that need to process a large number of culture dishes, the time cost will increase significantly. Utility Model Content

[0003] The purpose of the utility model is to solve the problem in the prior art that a tissue culture device can only add culture fluid or sealing oil to one culture dish at a time, and then switch to the next culture dish to add culture fluid or sealing oil, and cannot add culture fluid or sealing oil to different culture dishes, which increases the operation time and reduces the efficiency of addition. For experiments that need to process a large number of culture dishes, the time cost will increase significantly. The utility model proposes an organoid biological tissue culture device.

[0004] In order to achieve the above-mentioned purpose, the present invention adopts the following technical solutions: an organoid biological tissue culture device, comprising: a workbench and a mounting table, a plurality of injection tubes are fixedly penetrated at equal intervals on the top of the mounting table, a plurality of the bottoms of the injection tubes are fixedly connected to injection heads, a plurality of the tops of the injection tubes are fixedly connected to liquid extraction tubes, a plurality of the outer surfaces of the liquid extraction tubes are provided with quantitative pumps, a plurality of the outer surfaces of the injection tubes near the top are fixedly connected to flow regulating valves, a motor is fixedly connected to the inner top of the workbench, an output end of the motor is movably penetrated with the inner top of the workbench, a rotating shaft is fixedly connected to the output end of the motor, a turntable is fixedly connected to the top of the rotating shaft, a plurality of placement slots are equidistantly provided on the top of the turntable, a connecting rod is movably penetrated by the inner bottoms of the plurality of placement slots.

[0005] Preferably, the tops of the plurality of connecting rods are fixedly connected with round blocks, and the outer surfaces of the plurality of round blocks are respectively matched with the inner surfaces of the plurality of placement grooves.

[0006] Preferably, the bottoms of the plurality of connecting rods are fixedly connected with push blocks, and an arc-shaped groove is provided on the top of the workbench near the front side.

[0007] Preferably, a support rod movably penetrates the inner bottom of the arc-shaped groove, and a curved plate is fixedly connected to the top of the support rod.

[0008] Preferably, the outer surface of the arc-shaped plate matches the inner surface of the arc-shaped groove, and a first electric push rod is fixedly connected to the inner bottom of the workbench near the front side.

[0009] Preferably, the driving end of the first electric push rod is fixedly connected to the bottom of the support rod through a mounting plate, and the positions of the multiple injection heads match the positions of the multiple placement slots.

[0010] Preferably, a second electric push rod is fixedly connected to the rear surface of the workbench, and a driving end of the second electric push rod is fixedly connected to the bottom of the mounting platform.

[0011] Preferably, the position of the arc-shaped plate matches the positions of the plurality of push blocks, and the thickness of the arc-shaped plate is smaller than the depth of the arc-shaped groove.

[0012] Compared with the prior art, the advantages and positive effects of the present invention are:

[0013] 1. In the utility model, by using multiple injection tubes, injection heads, flow regulating valves, metering pumps and liquid extraction tubes in combination, multiple culture dishes can be injected and added at the same time, which can improve the injection efficiency, ensure the flow rate and flow of the liquid, and ensure accurate release and uniform distribution. In addition, the turntable, rotating shaft, support rod, first electric push rod, connecting rod, round block, arc plate and push plate are used in combination to quickly push out the culture dishes after addition in the placement tank, facilitate the removal of the culture dishes, and further improve the injection and addition efficiency of the culture dishes.

[0014] 2. In the present invention, when the arc plate is not in use, the arc plate can be moved into the arc groove to hide the arc plate to avoid affecting subsequent operations. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 A three-dimensional diagram of an organoid biological tissue culture device proposed in the present invention;

[0016] Figure 2 This is a side view of an organoid biological tissue culture device proposed in the present invention;

[0017] Figure 3 This is a partial structural expansion diagram of an organoid biological tissue culture device proposed in the utility model;

[0018] Figure 4 The present invention provides a partial structural cross-sectional view of an organoid biological tissue culture device.

[0019] Legend: 1. Workbench; 2. Placement slot; 3. Injection head; 4. Injection tube; 5. Flow control valve; 6. Suction tube; 7. Dosing pump; 8. Turntable; 9. Push block; 10. Motor; 11. First electric push rod; 12. Arc plate; 14. Mounting table; 15. Second electric push rod; 16. Arc slot; 17. Connecting rod; 18. Round block; 19. Rotating shaft; 20. Support rod. DETAILED DESCRIPTION

[0020] In order to more clearly understand the above-mentioned purpose, features and advantages of the present invention, the present invention is further described below with reference to the accompanying drawings and embodiments. It should be noted that the embodiments of the present application and the features therein can be combined with each other without conflict.

[0021] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways than those described herein. Therefore, the present invention is not limited to the specific embodiments disclosed in the following specification.

[0022] Example 1, as Figures 1-4 As shown, the utility model provides an organoid biological tissue culture device, comprising: a workbench 1 and a mounting table 14, a plurality of injection tubes 4 are fixedly penetrated at equal intervals on the top of the mounting table 14, a plurality of injection tubes 4 are fixedly connected to injection heads 3 at the bottoms thereof, a plurality of injection tubes 4 are fixedly connected to liquid extraction tubes 6 at the tops thereof, a quantitative pump 7 is provided on the outer surfaces of the plurality of liquid extraction tubes 6, a flow regulating valve 5 is fixedly connected to the outer surfaces of the plurality of injection tubes 4 near the tops thereof, a motor 10 is fixedly connected to the inner top of the workbench 1, an output end of the motor 10 is movably penetrated with the inner top of the workbench 1, a rotating shaft 19 is fixedly connected to the output end of the motor 10, a turntable 8 is fixedly connected to the top of the rotating shaft 19, a plurality of placement slots 2 are equidistantly provided on the top of the turntable 8, a plurality of connecting rods 17 are movably penetrated on the inner bottoms of the plurality of placement slots 2, and a plurality of connecting rods 17 are fixedly penetrated on the inner bottoms thereof. The top of each of the workbench 1 is fixedly connected with a round block 18, and the outer surfaces of the multiple round blocks 18 are respectively matched with the inner surfaces of the multiple placement grooves 2, and the bottoms of the multiple connecting rods 17 are fixedly connected with push blocks 9. An arc groove 16 is provided at the top of the workbench 1 near the front side, and a support rod 20 is movably passed through the inner bottom of the arc groove 16. The top of the support rod 20 is fixedly connected with an arc plate 12, and a first electric push rod 11 is fixedly connected to the inner bottom of the workbench 1 near the front side. The driving end of the first electric push rod 11 is fixedly connected to the bottom of the support rod 20 through the mounting plate. The positions of the multiple injection heads 3 are matched with the positions of the multiple placement grooves 2, and the rear surface of the workbench 1 is fixedly connected with a second electric push rod 15. The driving end of the second electric push rod 15 is fixedly connected to the bottom of the mounting table 14, and the position of the arc plate 12 is matched with the positions of the multiple push blocks 9.

[0023] The effect achieved by the entire embodiment 1 is that the four liquid extraction pipes 6 are connected to the sealing oil liquid tank or the culture liquid tank, and the flow regulating valve 5, the metering pump 7, the motor 10, the first electric push rod 11 and the second electric push rod 15 are electrically connected to the external PLC controller. The metering pump 7 can adopt a plunger metering pump, a screw metering pump, etc., and then the flow regulating valve 5 and the metering pump 7 are adjusted by the controller to adjust the flow and flow rate of the culture liquid and the sealing oil liquid to ensure their accurate release and uniform distribution, and ensure the accuracy of the culture. When the culture dish is injected with culture liquid or sealing oil liquid, the culture dish is placed on the turntable 8 along the placement slot 2, and then the turntable 8 is started to move the culture dish above the multiple injection heads 3, and then the second electric push rod 15 is started to drive the mounting table 14 to move downward, so that the injection head 3 moves into the culture dish, and then the metering pump 7 is started. The culture fluid or sealing oil liquid in the sealing oil tank or culture fluid tank is extracted and transported to the injection tube 4 through the liquid extraction tube 6, and the flow rate and flow velocity of the culture fluid and sealing oil liquid are adjusted through the flow regulating valve 5 and the quantitative pump 7, so that the culture fluid and sealing oil liquid can be accurately injected into the culture dish. By using four groups of injection heads 3 in combination, multiple culture dishes can be injected and added at the same time, which can improve the injection efficiency. After the injection is completed, the motor 10 can be started to rotate, driving the rotating shaft 19 and the turntable 8 to rotate, and the injected culture dish can be rotated to the outside, and then the first electric push rod 11 is started to drive the support rod 20 to move upward, so that the arc plate 12 moves upward, and the push block 9 is pushed upward. Under the action of the connecting rod 17, the round block 18 quickly pushes the culture dish out of the placement slot 2, which is convenient for taking out the culture dish and further improves the injection and addition efficiency of the culture dish.

[0024] Example 2, as Figures 1-4 As shown, the only difference between this embodiment and embodiment 1 is that the outer surface of the arc-shaped plate 12 matches the inner surface of the arc-shaped groove 16 , and the thickness of the arc-shaped plate 12 is smaller than the depth of the arc-shaped groove 16 .

[0025] The effect achieved by the entire embodiment 2 is that when the arc plate 12 is not in use, the arc plate 12 can be moved into the arc groove 16 to hide the arc plate 12 to avoid affecting subsequent operations.

[0026] Working principle: When injecting culture fluid or sealing oil into the culture dish, place the culture dish on the turntable 8 along the placement slot 2, then start the turntable 8 to move the culture dish above the multiple injection heads 3, then start the second electric push rod 15 to drive the mounting platform 14 to move downward, so that the injection head 3 moves into the culture dish, then start the quantitative pump 7, and extract the culture fluid or sealing oil from the sealing oil tank or culture fluid tank through the liquid extraction pipe 6 to the injection pipe 4, and adjust the flow rate and flow rate of the culture fluid and sealing oil through the flow regulating valve 5 and the quantitative pump 7, so that the culture fluid and sealing oil are accurately injected into the culture dish, and the four groups of injection heads are used. 3 can be used together to perform injection and addition work on multiple culture dishes at the same time, which can improve the injection efficiency. After the injection is completed, the motor 10 can be started to rotate and the injected culture dish can be rotated to the outside. Then, the first electric push rod 11 is started to drive the support rod 20 to move upward, so that the arc plate 12 moves upward, and the push block 9 is pushed upward. Under the action of the connecting rod 17, the round block 18 quickly pushes the culture dish out of the placement groove 2, which is convenient for taking out the culture dish and further improves the injection and addition efficiency of the culture dish. Then, the first electric push rod 11 is started to move the support rod 20 and the arc plate 12 into the arc groove 16.

[0027] The wiring diagram of the flow regulating valve 5, the metering pump 7, the motor 10, the first electric push rod 11, the second electric push rod 15 and the PLC controller in the present invention is common knowledge in the field, and its working principle is a well-known technology. The model is selected according to the actual use. Therefore, the control method and wiring arrangement of the flow regulating valve 5, the metering pump 7, the motor 10, the first electric push rod 11, the second electric push rod 15 and the PLC controller are no longer explained in detail.

[0028] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any other form. Any technician familiar with the profession may use the technical content disclosed above to change or modify it into an equivalent embodiment with equivalent changes for application in other fields. However, any simple modification, equivalent change and modification made to the above embodiment based on the technical essence of the present invention without departing from the content of the technical solution of the present invention shall still fall within the scope of protection of the technical solution of the present invention.

Claims

1. An organoid biological tissue culture device, characterized in that: include: A workbench (1) and a mounting table (14), wherein a plurality of injection tubes (4) are fixedly passed through the top of the mounting table (14) at equal intervals, the bottoms of the plurality of injection tubes (4) are fixedly connected to injection heads (3), the tops of the plurality of injection tubes (4) are fixedly connected to liquid extraction tubes (6), the outer surfaces of the plurality of liquid extraction tubes (6) are provided with quantitative pumps (7), the outer surfaces of the plurality of injection tubes (4) are fixedly connected to flow regulating valves (5) near the tops, the inner top of the workbench (1) is fixedly connected to a motor (10), the output end of the motor (10) is movably passed through the inner top of the workbench (1), the output end of the motor (10) is fixedly connected to a rotating shaft (19), the top of the rotating shaft (19) is fixedly connected to a turntable (8), the top of the turntable (8) is provided with a plurality of placement slots (2) at equal intervals, and the inner bottoms of the plurality of placement slots (2) are movably passed through by connecting rods (17).

2. The organoid biological tissue culture device according to claim 1, characterized in that: The tops of the plurality of connecting rods (17) are all fixedly connected with round blocks (18), and the outer surfaces of the plurality of round blocks (18) respectively match the inner surfaces of the plurality of placement grooves (2).

3. The organoid biological tissue culture device according to claim 2, characterized in that: The bottoms of the plurality of connecting rods (17) are all fixedly connected with push blocks (9), and an arc-shaped groove (16) is provided at a position near the front side of the top of the workbench (1).

4. The organoid biological tissue culture device according to claim 3, characterized in that: A support rod (20) movably penetrates the inner bottom of the arc-shaped groove (16), and the top of the support rod (20) is fixedly connected to the arc-shaped plate (12).

5. The organoid biological tissue culture device according to claim 4, characterized in that: The outer surface of the arc-shaped plate (12) matches the inner surface of the arc-shaped groove (16), and a first electric push rod (11) is fixedly connected to the inner bottom of the workbench (1) near the front side.

6. The organoid biological tissue culture device according to claim 5, characterized in that: The driving end of the first electric push rod (11) is fixedly connected to the bottom of the support rod (20) via a mounting plate, and the positions of the plurality of injection heads (3) match the positions of the plurality of placement slots (2).

7. The organoid biological tissue culture device according to claim 6, characterized in that: A second electric push rod (15) is fixedly connected to the rear surface of the workbench (1), and a driving end of the second electric push rod (15) is fixedly connected to the bottom of the mounting platform (14).

8. The organoid biological tissue culture device according to claim 7, characterized in that: The position of the arc-shaped plate (12) matches the positions of the plurality of push blocks (9), and the thickness of the arc-shaped plate (12) is smaller than the depth of the arc-shaped groove (16).

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