Graded filtering device for bovine serum albumin production

Abstract

The utility model relates to the technical field of bovine serum albumin production, in particular to a bovine serum albumin production grading filtering device which comprises a filtering part, a sample containing part arranged at the top of the filtering part and a sample sending part arranged at the bottom of the filtering part. The filter frame is detachably mounted at the bottom of the filter shell; the filtering frame is an annular frame, a chassis is fixed to the center of the filtering frame through a connecting rod, and a mounting frame is arranged on the chassis; a cylindrical first filter screen is arranged on the mounting frame, and a circular second filter screen is arranged at the bottom of the filter frame. The filter frame is arranged in the filter part, the circular second filter screen is arranged at the bottom of the filter frame, and the cylindrical first filter screen is arranged in the center of the filter part through the chassis and the mounting frame, so that protein to be filtered enters the central part of the mounting frame through the communicating pipe after being put in from the sample loading part; through the filtration of the first filter screen and the second filter screen in sequence, the requirement of graded filtration is met.

Description

Technical Field

[0001] The utility model relates to the technical field of bovine serum albumin production, in particular to a bovine serum albumin production grading and filtering device. Background Art

[0002] At present, the demand for bovine serum albumin in the market is increasing day by day. Faced with the background of low efficiency and poor quality of traditional filtration methods, a more convenient and efficient production method has emerged. The sodium octanoate method is currently a better way to produce bovine serum albumin on a large scale. Therefore, it is particularly important to use this method well and make it more convenient for us to use. Therefore, based on the existing technology, it is necessary to design a bovine serum albumin production graded filtration device to cooperate with the above purification method to achieve large-scale bovine serum albumin production. Utility Model Content

[0003] In order to solve the above problems, the utility model provides a bovine serum albumin production grading filtration device, comprising a filtration part, a sample loading part arranged at the top of the filtration part, and a sample sending part arranged at the bottom of the filtration part, the filtration part comprising a filter shell and a filter frame, the filter shell is a cylinder with an opening at the lower end, and the filter frame is detachably installed at the bottom of the filter shell; the filter frame is an annular frame, and a chassis is fixed at the center of the filter frame by a connecting rod, and a mounting frame is provided on the chassis; a cylindrical first filter screen is provided on the mounting frame, and a circular second filter screen is provided at the bottom of the filter frame.

[0004] Furthermore, the filter frame is provided with thread grooves at the top and bottom for threaded connection with the filter housing and the hair sampling part.

[0005] Furthermore, a guide ring is provided on the annular inner side of the thread groove of the filter frame. A sealing layer is provided on one side of the guide ring close to the thread groove, and an inclined surface is provided on the other side.

[0006] Furthermore, the mounting frame includes a mounting ring and a mounting rod, the two ends of the mounting rod are respectively connected to the mounting ring and the chassis, and the first filter is attached to the mounting ring and the mounting rod to maintain a cylindrical shape.

[0007] Furthermore, the upper end surface of the chassis is a convex surface.

[0008] Furthermore, the sample loading part and the filter part are connected through a connecting pipe, the top of the connecting pipe is connected to the bottom of the sample loading part and is provided with an electric control valve, and the bottom of the connecting pipe extends under the mounting ring.

[0009] Furthermore, the sample generating portion includes a conical flow guide portion connected to the filter portion and a sample loading tank detachably connected to the flow guide portion.

[0010] In summary, the present invention has at least one of the following beneficial effects:

[0011] The utility model sets a filter frame in the filter part, sets a circular second filter screen at the bottom of the filter frame, and sets a cylindrical first filter screen in the center of the filter part through a chassis and a mounting frame. After the protein to be filtered is put into the sample loading part, it enters the central part of the mounting frame through a connecting pipe, and is filtered by the first filter screen and the second filter screen in turn, thereby achieving the demand for graded filtration.

[0012] The filter part of the utility model adopts an integrated design. The first filter screen and the second filter screen are both arranged on the filter frame and are detachably connected to other components, which makes it easy to replace, clean and maintain the filter screens. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0014] Figure 1 This is a schematic diagram of the structure of the utility model;

[0015] Figure 2 This is a schematic diagram of the internal structure of the utility model;

[0016] Figure 3 Schematic diagram of the filter frame structure;

[0017] Figure 4 for Figure 3 Top view. DETAILED DESCRIPTION

[0018] The following is combined with Figure 1-4 The utility model is described in further detail.

[0019] Example 1:

[0020] This embodiment provides a bovine serum albumin production grading filtration device, which includes, from top to bottom, a sample loading part 2, a filtration part 1 and a sample generating part 3. The various parts are all detachably connected, and the specific connection method can be a threaded connection or other connection method that can meet the sealing performance.

[0021] The sample loading section 2 is a cylindrical transparent container with volumetric graduations on its surface to meet the needs of initial quantitative experiments in bovine serum albumin fractional filtration. The bottom of the sample loading section 2 extends into the interior of the filtration section 1 through a connecting tube 21. An electrically controlled valve 22 is installed at the connection between the connecting tube 21 and the sample loading section 2. When the solution in the sample loading section 2 needs to be measured, the electrically controlled valve 22 is closed to prevent the protein solution from flowing out directly. When continuous production is required, the electrically controlled valve 22 is opened to continuously input the protein solution to be filtered from the sample loading section 2.

[0022] Filtering portion 1 comprises filter housing 11 and filter frame 12, and described filter housing 11 is the cylindrical shell of lower end opening, and filter frame 12 is annular frame, is provided with screw thread groove up and down, is used for being threadedly connected with filter housing 11 and hair sample portion 3.Filtering frame 12 centers are fixed with chassis 122 by connecting rod 121, described chassis 122 is provided with mounting frame 123, mounting frame 123 comprises mounting ring 127 and mounting rod 128, described mounting rod 128 two ends connect mounting ring 127 and chassis 122 respectively, thereby the first filter screen 124 is attached to mounting ring 127 and mounting rod 128 and maintains cylindrical.The second filter screen 125 is then installed in the bottom of filter frame 12.Communication pipe 21 is sent into the first filter screen 124 insides with protein liquid to be filtered, and protein liquid passes through the second filter screen 125 secondary filtration in bottom again after the first filter screen filters earlier.For avoiding protein liquid to stick on the chassis 122, in the present embodiment, chassis 122 upper end faces are set to convex surface. Similarly, in order to prevent the protein liquid from flowing into the connecting gap between the filter housing 11 and the filter frame 12, a guide ring 126 is provided on the annular inner side of the thread groove 129 of the filter frame 12. The cross-section of the guide ring 126 is a right triangle, and a sealing layer is provided on the vertical side of the guide ring 126 close to the thread groove, and the other side is an inclined surface. The protein liquid remaining on the filter housing 11 can flow downward along the inclined surface into the second filter screen 125.

[0023] The sample dispensing unit 3 includes a tapered flow guide 31 connected to the filter unit 1 and a sample tank 32 detachably connected to the flow guide 31. The upper end of the tapered flow guide 31 is provided with threads that cooperate with the thread groove 129 and is provided with sealing measures. The protein solution after the secondary filtration of the second filter screen 125 flows through the flow guide 31 into the sample tank 32 at the bottom for collection.

[0024] The utility model sets a filter frame in the filter part, sets a circular second filter screen at the bottom of the filter frame, and sets a cylindrical first filter screen in the center of the filter part through a chassis and a mounting frame. After the protein to be filtered is put into the sample loading part, it enters the central part of the mounting frame through a connecting pipe, and is filtered by the first filter screen and the second filter screen in turn, thereby achieving the demand for graded filtration.

[0025] The filter part of the utility model adopts an integrated design. The first filter screen and the second filter screen are both arranged on the filter frame and are detachably connected to other components, which makes it easy to replace, clean and maintain the filter screens.

[0026] The above are all preferred embodiments of the present invention, and are not intended to limit the scope of protection of the present invention. Therefore, any equivalent changes made based on the structure, shape, and principle of the present invention should be included in the scope of protection of the present invention.

Claims

1. A bovine serum albumin production graded filtration device, comprising a filtration portion (1), a sample loading portion (2) disposed at the top of the filtration portion (1), and a sample generating portion (3) disposed at the bottom of the filtration portion (1), characterized in that: The filter portion (1) comprises a filter housing (11) and a filter frame (12); the filter housing (11) is a cylindrical body with an open lower end; the filter frame (12) is detachably mounted on the bottom of the filter housing (11); the filter frame (12) is an annular frame; a base plate (122) is fixed to the center of the filter frame (12) via a connecting rod (121); a mounting frame (123) is provided on the base plate (122); a cylindrical first filter screen (124) is provided on the mounting frame (123); and a circular second filter screen (125) is provided at the bottom of the filter frame (12).

2. A bovine serum albumin production grading filtration device according to claim 1, characterized in that: The filter frame (12) is provided with thread grooves (129) at the top and bottom for threaded connection with the filter housing (11) and the sample generating portion (3).

3. The bovine serum albumin production grading filtration device according to claim 2, characterized in that: A guide ring (126) is provided on the inner annular side of the thread groove of the filter frame (12); a sealing layer is provided on one side of the guide ring (126) close to the thread groove, and an inclined surface is provided on the other side.

4. The bovine serum albumin production grading filtration device according to claim 1, characterized in that: The mounting frame (123) comprises a mounting ring (127) and a mounting rod (128), the two ends of the mounting rod (128) respectively connecting the mounting ring (127) and the chassis (122), and the first filter (124) is attached to the mounting ring (127) and the mounting rod (128) to maintain a cylindrical shape.

5. The bovine serum albumin production grading filtration device according to claim 4, characterized in that: The upper end surface of the chassis (122) is a convex surface.

6. The bovine serum albumin production grading filtration device according to claim 4, characterized in that: The sample loading portion (2) and the filter portion (1) are connected via a connecting pipe (21). The top of the connecting pipe (21) is connected to the bottom of the sample loading portion (2) and is provided with an electric control valve (22). The bottom of the connecting pipe (21) extends below the mounting ring (127).

7. The bovine serum albumin production grading filtration device according to claim 1, characterized in that: The sample generating portion (3) comprises a conical flow guide portion (31) connected to the filter portion (1) and a sample loading tank (32) detachably connected to the flow guide portion (31).

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