Carrier removal filter for DNA (Deoxyribose Nucleic Acid) extraction experiment

Abstract

The utility model discloses a carrier removal filter for DNA extraction experiment, which relates to the technical field of DNA extraction, and comprises an outer limiting sleeve, a limiting structure is embedded in the outer limiting sleeve, the inner side of the outer limiting sleeve is fixedly connected with a limiting layer, the inner wall of the outer limiting sleeve is fixedly provided with a limiting cavity at the same time, the bottom end of the outer limiting sleeve is fixedly connected with a base, and the outer limiting sleeve is fixedly connected with the base. A bottom connecting rod is fixedly connected to the top end of the base and fixedly connected with the limiting structure at the same time. The effect of surrounding the whole limiting structure is achieved through the outer limiting sleeve, the effect of fixing and supporting the outer limiting sleeve is achieved through the base, and the effect of limiting and fixing the whole limiting structure on the inner side of the outer limiting sleeve is achieved through the limiting layer; and the effect of limiting and fixing the filter embedded between the limiting structure and the outer limiting sleeve is achieved through the limiting cavity.

Description

Technical Field

[0001] The utility model relates to the field of DNA extraction, in particular to a carrier removal filter for DNA extraction experiments. Background Art

[0002] In DNA extraction experiments, the processed sample is placed in a decarrier filter, lysing solution is added, and after lysis is completed, it is centrifuged at high speed for 3 minutes. At this time, the DNA will pass through the filter membrane built into the decarrier filter and precipitate to the bottom of the tube. The user can then remove the sample in the upper sleeve of the carrier filter and recover the liquid at the bottom of the tube. However, the existing decarrier filter used in DNA extraction experiments has the following problems during the extraction operation:

[0003] During the extraction operation, only a single de-carrier filter can be centrifuged, resulting in low extraction efficiency. Some methods use a limiting frame in an external centrifugal device to uniformly limit and fix multiple de-carrier filters. However, this limiting frame has a simple structure and is less stable when centrifuging multiple de-carrier filters after limiting them. It is difficult to ensure the unified centrifugal operation of multiple de-carrier centrifuges, which in turn affects the extraction operation.

[0004] Therefore, the utility model provides a carrier removal filter for DNA extraction experiments. Utility Model Content

[0005] In view of the deficiencies in the prior art, the utility model provides a carrier removal filter for DNA extraction experiments, which solves the problems raised by the above-mentioned background technology.

[0006] To achieve the above objectives, the utility model is implemented through the following technical solutions: a carrier removal filter for DNA extraction experiments, comprising an outer limiting sleeve, a limiting structure embedded inside the outer limiting sleeve, the inner side of the outer limiting sleeve fixedly connected to the limiting layer, the inner wall of the outer limiting sleeve is also fixedly provided with a limiting cavity, the bottom end of the outer limiting sleeve is fixedly connected to the base, the top of the base is fixedly connected to the bottom connecting rod, and the bottom connecting rod is also fixedly connected to the limiting structure, the limiting structure comprises a first limiting plate and a second limiting plate arranged in an upper and lower distribution, the outer sides of the first limiting plate and the second limiting plate are fixedly connected to the outer layer, the bottoms of the first limiting plate and the second limiting plate are fixedly connected to auxiliary supports, the bottom end of the first limiting plate is fixedly connected to the sleeve, the top of the second limiting plate is fixedly connected to the sleeve rod, the surface of the base is provided with a limiting groove, and the outer surfaces of the first limiting plate and the second limiting plate are provided with positioning parts.

[0007] As a further technical solution of the present invention, the outer limit sleeve is arranged vertically in a cylindrical shape as a whole, and the bottom end is clamped with the base. The surface edge of the base is also provided with an annular clamping groove corresponding to the bottom end of the outer limit sleeve. Multiple groups of limit layers are arranged along the inner side of the outer limit sleeve, and the overall shape is an inward concave arc protrusion. The limit layer is also located on the outside of the positioning part.

[0008] As a further technical solution of the present invention, the limiting cavity is an outwardly convex arc-shaped cavity vertically arranged and spaced between multiple groups of limiting layers.

[0009] As a further technical solution of the present invention, the positioning part is arranged in an arc-shaped concave shape, and multiple groups of them are evenly distributed along the edge positions of the first limiting plate and the second limiting plate. The outer layer is arranged in an arc-shaped rubber layer, which is adjacent to the positioning part, and the surface of the outer layer is arranged in a tooth-like protrusion.

[0010] As a further technical solution of the present invention, the auxiliary support is located directly below the positioning portion and is arranged in the form of an arc-shaped plastic seat with a sponge layer provided on its surface.

[0011] As a further technical solution of the present invention, the sleeve and the sleeve rod are vertically fixedly sleeved, and the first limiting plate and the second limiting plate are fixedly connected in upper and lower positions through the sleeve and the sleeve rod.

[0012] As a further technical solution of the present invention, the bottom connecting rod and the bottom end of the second limiting plate are fixedly sleeved.

[0013] The utility model provides a carrier removal filter for DNA extraction experiments, which has the following beneficial effects compared with the prior art:

[0014] 1. This design is a carrier removal filter for DNA extraction experiments. The outer limiting sleeve is used to surround the entire limiting structure, the base is used to fix and support the outer limiting sleeve, the limiting layer is used to limit and fix the entire limiting structure on the inner side of the outer limiting sleeve, and the limiting cavity is used to limit and fix the filter embedded between the limiting structure and the outer limiting sleeve.

[0015] 2. This design is for a carrier removal filter used in DNA extraction experiments. The positioning portion is used to facilitate the placement of the filter. The outer layer is used to achieve contact with the inner wall of the outer limiting sleeve, thereby enhancing the stability between the overall limiting structure and the outer limiting sleeve. The auxiliary positioning portion is used to limit the filter through the auxiliary support. At the same time, the sponge layer on the surface protects the filter. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of a carrier removal filter used in DNA extraction experiments;

[0017] Figure 2 This is an exploded diagram of the limiting structure connection of a carrier removal filter used in DNA extraction experiments;

[0018] Figure 3 A carrier removal filter for DNA extraction experiments Figure 2 Enlarged view of the structure at point B in the middle.

[0019] In the figure: 1. External limiting sleeve; 2. Limiting structure; 3. Limiting layer; 4. Limiting cavity; 5. Base; 6. Bottom connecting rod; 7. First limiting plate; 8. Second limiting plate; 9. External layer; 10. Auxiliary support; 11. Sleeve; 12. Sleeve rod; 13. Limiting groove; 14. Positioning part. DETAILED DESCRIPTION

[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0021] See also Figure 1-3 The utility model provides a technical solution for removing carrier filters for DNA extraction experiments: it includes an outer limiting sleeve 1, a limiting structure 2 is embedded in the outer limiting sleeve 1, the inner side of the outer limiting sleeve 1 is fixedly connected to the limiting layer 3, and the inner wall of the outer limiting sleeve 1 is fixedly provided with a limiting cavity 4 at the same time. The bottom end of the outer limiting sleeve 1 is fixedly connected to the base 5, and the top of the base 5 is fixedly connected to the bottom connecting rod 6. The bottom connecting rod 6 is also fixedly connected to the limiting structure 2. The limiting structure 2 includes a first limiting plate 7 and a second limiting plate 8 arranged in an upper and lower distribution. The outer sides of the first limiting plate 7 and the second limiting plate 8 are fixedly connected to the outer layer 9, and the bottoms of the first limiting plate 7 and the second limiting plate 8 are fixedly connected to an auxiliary support 10, the bottom end of the first limiting plate 7 is fixedly connected to the sleeve 11, and the top of the second limiting plate 8 is fixedly connected to the sleeve rod 12. The surface of the base 5 is provided with a limiting groove 13, and the outer surfaces of the first limiting plate 7 and the second limiting plate 8 are provided with positioning parts 14.

[0022] like Figure 1As shown, the outer limiting sleeve 1 is vertically arranged in a cylindrical shape as a whole, and the bottom end and the base 5 are clamped together. The surface edge of the base 5 is also provided with an annular clamping groove corresponding to the bottom end of the outer limiting sleeve 1. Multiple groups of limiting layers 3 are arranged along the inner side of the outer limiting sleeve 1, and the overall inner concave arc protrusion is arranged. The limiting layer 3 is also located on the outside of the positioning part 14, and the limiting cavity 4 is vertically arranged in an outer convex arc cavity, and is spaced between multiple groups of limiting layers 3. The outer limiting sleeve 1 is used to enclose the entire limiting structure 2, and the base 5 is used to fix and support the outer limiting sleeve 1. The limiting layer 3 is used to limit and fix the limiting structure 2 as a whole on the inner side of the outer limiting sleeve 1, and the limiting cavity 4 is used to limit and fix the filter embedded between the limiting structure 2 and the outer limiting sleeve 1.

[0023] After the user has installed the external multiple sets of filters and the limiting structure 2, the entire limiting structure 2 together with the filter is placed into the outer limiting sleeve 1, so that the bottom end of the outer limiting sleeve 1 and the base 5 are clamped and fixed, and the limiting layer 3 assists in limiting the outside of the limiting structure 2. The limiting cavity 4 is located outside the filter in the limiting structure 2, and the filter in the limiting structure 2 is limited and fixed to ensure the placement stability of the filter in the limiting structure 2, so as to facilitate subsequent centrifugal operations.

[0024] like Figure 2 The positioning portion 14 shown is arranged in an arc-shaped inward concave shape, and multiple groups are equidistantly distributed along the edge positions of the first limiting plate 7 and the second limiting plate 8. The outer layer 9 is arranged in an arc-shaped rubber layer, which is adjacent to the positioning portion 14. The surface of the outer layer 9 is arranged in a tooth-like protrusion. The auxiliary support 10 is located directly below the positioning portion 14 and is arranged in an arc-shaped plastic seat. A sponge layer is provided on its surface. The positioning portion 14 is used to facilitate the placement and positioning of the filter. The outer layer 9 is used to contact the inner wall of the outer limiting sleeve 1 to enhance the stability between the limiting structure 2 as a whole and the outer limiting sleeve 1. The auxiliary support 10 is used to achieve the effect of limiting the filter by the auxiliary positioning portion 14. At the same time, the sponge layer on the surface protects the filter.

[0025] The user places the filter into the limiting structure 2 according to the positioning part 14, and the first limiting plate 7 and the second limiting plate 8 cooperate with the respective positioning parts 14 from the upper and lower positions to realize the limiting processing of the filter. At the same time, the auxiliary support 10 assists in limiting and fixing the filter from below the positioning part 14 to provide buffering protection. Finally, the user places the entire limiting structure 2 into the outer limiting sleeve 1, and the outer layer 9 contacts the inner wall of the outer limiting sleeve 1 to enhance the stability of the limiting structure 2 in the outer limiting sleeve 1.

[0026] like Figure 2As shown, the sleeve 11 and the sleeve rod 12 are fixedly sleeved vertically, the first limit plate 7 and the second limit plate 8 are fixedly connected at the upper and lower positions through the sleeve 11 and the sleeve rod 12, and the bottom connecting rod 6 and the bottom end of the second limit plate 8 are fixedly sleeved.

[0027] The working principle of the present invention is as follows: when using the device, after adding the sample and the lysis solution into the filter, the user first places the filter into the limiting structure 2 according to the position of the positioning part 14, and the bottom of the filter is supported in the limiting groove 13. At this time, the first limiting plate 7 and the second limiting plate 8 limit the filter from the upper and lower positions. Then the user places the entire limiting structure 2 into the outer limiting sleeve 1, so that the bottom end of the outer limiting sleeve 1 and the base 5 are clamped and fixed. At this time, the filter is located between the outer limiting sleeve 1 and the limiting structure 2 in a limiting state. Finally, the user places the entire device into an external centrifugal device for centrifugal operation. After the operation is completed, the sample in the upper sleeve of the filter is removed and the liquid at the bottom can be recovered.

[0028] The above description is merely a preferred embodiment of the present invention. It should be noted that those skilled in the art may make various improvements and modifications without departing from the principles of the present invention, and such improvements and modifications should be considered within the scope of protection of the present invention. Structures, devices, and operating methods not specifically described or explained in this invention shall, unless otherwise specified or limited, be implemented in accordance with conventional means in the art.

Claims

1. A carrier removal filter for DNA extraction experiments, characterized in that: The invention comprises an outer limiting sleeve (1), a limiting structure (2) is embedded in the inner part of the outer limiting sleeve (1), a limiting layer (3) is fixedly connected to the inner side of the outer limiting sleeve (1), a limiting cavity (4) is fixedly provided on the inner wall of the outer limiting sleeve (1), the bottom end of the outer limiting sleeve (1) is fixedly connected to a base (5), the top end of the base (5) is fixedly connected to a bottom connecting rod (6), the bottom connecting rod (6) is fixedly connected to the limiting structure (2), and the limiting structure (2) comprises a first limiting plate ( 7) and a second limiting plate (8), the outer sides of the first limiting plate (7) and the second limiting plate (8) are fixedly connected with an outer sticking layer (9), the bottoms of the first limiting plate (7) and the second limiting plate (8) are fixedly connected with an auxiliary support (10), the bottom end of the first limiting plate (7) is fixedly connected with a sleeve (11), the top end of the second limiting plate (8) is fixedly connected with a sleeve rod (12), the surface of the base (5) is provided with a limiting groove (13), and the outer surfaces of the first limiting plate (7) and the second limiting plate (8) are provided with a positioning portion (14).

2. The carrier removal filter for DNA extraction experiments according to claim 1, characterized in that: The outer limiting sleeve (1) is vertically arranged in a cylindrical shape as a whole, and the bottom end is clamped with the base (5). The surface edge of the base (5) is provided with an annular clamping groove corresponding to the bottom end of the outer limiting sleeve (1). The limiting layer (3) is arranged in multiple groups along the inner side of the outer limiting sleeve (1), and is protruded in an inwardly concave arc shape as a whole. The limiting layer (3) is also located outside the positioning portion (14).

3. The carrier removal filter for DNA extraction experiments according to claim 1, characterized in that: The limiting cavity (4) is in the form of an outwardly convex arc-shaped cavity, which is vertically arranged and spaced between the plurality of groups of limiting layers (3).

4. The carrier removal filter for DNA extraction experiments according to claim 1, characterized in that: The positioning portion (14) is arranged in an arc-shaped inward concave shape, and is evenly distributed in multiple groups along the edge positions of the first limiting plate (7) and the second limiting plate (8). The outer layer (9) is arranged as an arc-shaped rubber layer and is adjacent to the positioning portion (14). The surface of the outer layer (9) is arranged in a tooth-shaped protrusion.

5. The carrier removal filter for DNA extraction experiments according to claim 1, characterized in that: The auxiliary support (10) is located directly below the positioning portion (14) and is provided in the form of an arc-shaped plastic seat, with a sponge layer provided on its surface.

6. The carrier removal filter for DNA extraction experiments according to claim 1, characterized in that: The sleeve (11) and the sleeve rod (12) are vertically fixedly sleeved, and the first limiting plate (7) and the second limiting plate (8) are fixedly connected in upper and lower positions through the sleeve (11) and the sleeve rod (12).

7. The carrier removal filter for DNA extraction experiments according to claim 1, characterized in that: The bottom connecting rod (6) and the bottom end of the second limiting plate (8) are fixedly sleeved.

Images