Gene sequencing electrophoresis tank

Abstract

A gene sequencing electrophoresis tank comprises a main tank body and a cover body on the upper side of the main tank body, a positive electrode end and a negative electrode end are arranged in the main tank body, a positive terminal and a negative terminal which can be in contact communication with the positive electrode end and the negative electrode end respectively are arranged on the cover body, and a fixable glue tank is hinged to the middle of the bottom of the main tank body. The middle of the bottom of the main tank body is further provided with a driving mechanism capable of driving the glue tank fixing mechanism to turn upwards, and when a glue plate is placed in the main tank body and when the glue plate is taken out after electrophoresis is completed, the glue tank fixing mechanism can be driven to turn upwards through the driving mechanism. And one side or all of the glue groove fixing table is exposed out of the electrophoresis liquid, the taking and placing work of the glue plate is completed, the hands do not need to stretch into the electrophoresis blade, and the taking and placing of the glue plate can be safer and more convenient.

Description

Technical Field

[0001] The utility model relates to the technical field of electrophoresis tanks, in particular to a gene sequencing electrophoresis tank. Background Art

[0002] The electrophoresis tank is the core part of the gel electrophoresis system. It uses the electric field to make charged particles move in the medium, thereby achieving sample separation and analysis. When in use, the gel is placed between two buffer chambers, and the electric field connects the two buffer chambers through the gel. When the electric field is applied to the electrophoresis fluid, the charged particles in the liquid (such as proteins, nucleic acids, etc.) will be affected by the electric field force and move toward the electrodes. According to factors such as particle size, charge and medium, different particles will move at different speeds, thereby achieving separation. However, when in use, the gel needs to be completely immersed in the electrophoresis fluid. After the electrophoresis is completed, the gel needs to be removed for further observation, which makes it inconvenient to take and put the gel. In particular, after the electrophoresis is completed, the electrophoresis fluid usually has a higher temperature, which further increases the difficulty of removing the gel. Utility Model Content

[0003] In order to solve the technical problems existing in the above-mentioned background technology, the utility model provides a gene sequencing electrophoresis tank.

[0004] The technical solution of this utility model is as follows:

[0005] A gene sequencing electrophoresis tank comprises a main tank body and a cover body on its upper side. Opposite sides of the bottom of the main tank body extend downward to form a transition cavity, and a positive electrode terminal and a negative electrode terminal are respectively provided in the two transition cavities. The cover body is provided with a positive terminal and a negative terminal that can respectively contact and communicate with the positive electrode terminal and the negative electrode terminal. A molded rubber plate can be placed in the middle of the bottom of the main tank body, and the electrophoresis operation is completed by supplying power to the positive terminal and the negative terminal.

[0006] As the core technical concept of the present invention, a glue tank fixing mechanism is provided in the middle of the bottom of the main tank body. Specifically, a strip-shaped installation groove is opened in the middle of the bottom of the main tank body. The glue tank fixing mechanism includes a mounting rod and a glue tank fixing platform on the outside of one end thereof, and the glue tank fixing platform can complete the fixing work of the gel plate and the glue tank (gel forming tank). The end of the mounting rod away from the glue tank fixing platform is hinged to one end inside the mounting groove and can be rotated into the mounting groove. The middle of the bottom of the main tank body is also provided with a driving mechanism that can drive the glue tank fixing mechanism to flip up. On the basis of the above structure, when placing the glue plate into the main tank body, and when the glue plate is taken out after electrophoresis, the driving mechanism can drive the glue tank fixing mechanism to flip up, so that one side or all of the glue tank fixing platform is exposed to the electrophoresis liquid, and the glue plate is taken out to complete the work of taking and placing the glue plate without reaching into the electrophoresis blade, so that the taking and placing of the glue plate can be safer and more convenient.

[0007] As described above, a gene sequencing electrophoresis tank, specifically, includes a threaded rod arranged inside the mounting groove along the direction of the mounting groove, and a wedge block spirally connected to the threaded rod is slidably provided in the mounting groove, which is configured to lift the glue tank fixing mechanism by sliding. One end of the threaded rod is also vertically provided with a driving rod whose lower end is transmission-connected to it, and the upper end of the driving rod can extend the electrophoresis liquid. On this basis, only by rotating the upper end of the driving rod, the wedge block can be driven to slide by the threaded rod to complete the up / down work of the glue tank fixing mechanism, so that the driving work of the glue tank fixing mechanism can be more conveniently realized.

[0008] As a preferred embodiment, a knob is further provided at the upper end of the driving rod, so that the rotary driving operation of the driving rod can be more convenient and labor-saving.

[0009] In the gene sequencing electrophoresis tank as described above, the mounting rod includes two strips arranged opposite to each other, and the threaded rod and the wedge block are both located between the two strips, so that the arrangement of the threaded rod and the wedge block will not affect the storage work of the mounting rod into the mounting groove, thereby reducing the space occupied by the drive mechanism installation.

[0010] As a preferred embodiment, the glue tank fixing platform is configured to be able to rotate to fit horizontally with the bottom of the main tank body near the mounting rod, ensuring that the electrophoresis work can be completed more quickly and effectively under the action of the positive electrode end and the negative electrode end.

[0011] As a further preferred embodiment, a convex column is provided on the inner side of the strip away from the end of the glue tank fixing platform, and the wedge block includes an inclined surface and a vertical surface. It is configured to lift the mounting rod through the cooperation of the inclined surface and the convex column when sliding, and to press against one side of the glue tank fixing platform through the vertical surface when sliding in the opposite direction, so that the wedge block can not only support the reversal of the glue tank fixing mechanism, but also play a certain clamping role on the glue tank fixing platform when the electrophoresis work is in progress, thereby ensuring that the electrophoresis work can proceed smoothly.

[0012] As described above, a gene sequencing electrophoresis tank, in order to ensure the fixing effect of the gel tank fixing platform on the gel plate, the gel tank fixing platform includes a bottom plate, and a fixing platform and an elastic extrusion platform are relatively provided on the side away from the mounting rod, and the gel tank can be elastically squeezed between the fixing platform and the elastic extrusion platform.

[0013] As described above, a gene sequencing electrophoresis tank further includes a cooling water tank to cool the main tank body during the electrophoresis process. The main tank body is arranged in the cooling water tank, and the plane where the upper end is located is not lower than the plane where the upper end of the cooling water tank is located.

[0014] The beneficial effect of the utility model is that: the utility model is a gene sequencing electrophoresis tank, which can drive the tank fixing mechanism to flip up through the driving mechanism when placing the gel plate into the main tank body, and when taking out the gel plate after electrophoresis is completed, so that one side or the entire gel tank fixing platform is exposed to the electrophoresis liquid, completing the gel plate taking and placing work, without having to reach into the electrophoresis blade, making the gel plate taking and placing safer and more convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] By reading the detailed description of the preferred embodiment below, the solutions and advantages of the present application will become clear to those skilled in the art. The accompanying drawings are only used to illustrate the preferred embodiment and are not to be considered as limiting the present invention.

[0016] In the attached figure:

[0017] Figure 1 Schematic diagram of the structure of the electrophoresis tank in the embodiment;

[0018] Figure 2 Schematic diagram of the internal structure of the electrophoresis tank in the embodiment;

[0019] Figure 3 Schematic diagram of the structure of the inner groove in the embodiment (when the wedge is pressed against the rubber groove fixing platform);

[0020] Figure 4 Schematic diagram of the structure of the inner groove in the embodiment (when the wedge block lifts up the glue groove fixing mechanism);

[0021] Figure 5 Schematic diagram of the structure of the driving mechanism in the embodiment;

[0022] Figure 6 Schematic diagram of the cooperation structure between the wedge block and the glue groove fixing mechanism in the embodiment;

[0023] The components represented by the reference numerals in the figure are:

[0024] 1. Outer tank; 11. Cooling water tank; 12. Water inlet pipe; 13. Water outlet pipe; 2. Inner tank; 21. Main tank body; 22. Positive electrode terminal; 23. Negative electrode terminal; 24. Glue tank fixing mechanism; 241. Mounting rod; 242. Glue tank fixing platform; 243. Boss; 25. Driving mechanism; 251. Driving box; 252. Threaded rod; 253. Wedge; 254. Driving rod; 3. Upper cover; 31. Cover body; 32. Positive terminal; 33. Negative terminal. DETAILED DESCRIPTION

[0025] Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings.

[0026] Example

[0027] This embodiment provides a gene sequencing electrophoresis tank, see Figure 1-Figure 3 , including an inner tank 2 and an upper cover 3 on its upper side, the inner tank 2 includes a main tank body 21, the upper cover 3 includes a cover body 31 buckled on the upper side of the main tank body 21, the opposite sides of the bottom of the main tank body 21 extend downward to form a transition cavity, and the two transition cavities are respectively provided with a positive electrode terminal 22 and a negative electrode terminal 23, the upper cover 3 also includes a positive terminal 32 and a negative terminal 33 provided on the cover body 31 and capable of contacting and communicating with the positive electrode terminal 22 and the negative electrode terminal 23 respectively when buckled on the upper side of the main tank body 21, the formed rubber plate can be placed in the middle of the bottom of the main tank body 21, and the electrophoresis operation is completed by supplying power to the positive terminal 32 and the negative terminal 33. The structure of the electrophoresis tank (the above-mentioned gene sequencing electrophoresis tank) is described in detail below.

[0028] In this embodiment, as the core technical concept of the present invention, a glue groove fixing mechanism 24 is provided in the middle of the bottom of the main tank body 21. Specifically, a strip-shaped mounting groove is opened in the middle of the bottom of the main tank body 21. The glue groove fixing mechanism 24 includes a mounting rod 241 and a glue groove fixing platform 242 on the outside of one end thereof. The fixing work of the gel rubber plate and the glue groove can be completed by the glue groove fixing platform 242. The end of the mounting rod 241 away from the glue groove fixing platform 242 is hinged to one end inside the mounting groove and can be rotated into the mounting groove. The middle of the bottom of the main tank body 21 is also provided with a driving mechanism 25 that can drive the glue groove fixing mechanism 24 to flip up. Figure 4 On the basis of the above structure, when placing the gel sheet into the main tank body 21, and when taking out the gel sheet after electrophoresis, the driving mechanism 25 can drive the gel tank fixing mechanism 24 to flip up, so that one side or the entire gel tank fixing platform 242 is exposed to the electrophoresis liquid, and the gel sheet can be taken in and out without having to reach into the electrophoresis blade, making the taking in and out of the gel sheet safer and more convenient.

[0029] Further, combined with Figure 5The wedge 253 is driven by the threaded rod 252 to slide in the mounting groove, and the wedge 253 is driven by the threaded rod 252 to slide in the mounting groove, so that the glue groove fixing mechanism 24 can be lifted up by sliding.

[0030] As a preferred embodiment, a knob is further provided at the upper end of the driving rod 254, so that the rotation driving work of the driving rod 254 can be more convenient and labor-saving.

[0031] In combination with the structure of the driving mechanism 25, with regard to the structure of the glue groove fixing mechanism 24, the mounting rod 241 includes two strips arranged opposite to each other, and the threaded rod 252 and the wedge block 253 are both located between the two strips, so that the setting of the threaded rod 252 and the wedge block 253 will not affect the storage work of the mounting rod 241 into the mounting groove, thereby reducing the installation space occupied by the driving mechanism 25.

[0032] As a preferred embodiment, the glue tank fixing platform 242 is configured to be able to rotate to horizontally fit with the bottom of the main tank body 21 near the mounting rod 241, ensuring that under the action of the positive electrode end 22 and the negative electrode end 23, the electrophoresis work can be completed more quickly and effectively.

[0033] As a further preferred combination Figure 6 A boss 243 is provided on the inner side of the strip away from the end of the glue tank fixing platform 242. The wedge block 253 includes an inclined surface and a vertical surface. It is configured to lift the mounting rod 241 through the cooperation of the inclined surface and the boss 243 when sliding, and can be pressed against one side of the glue tank fixing platform 242 through the vertical surface when sliding in the opposite direction, so that the wedge block 253 can not only support the reversal of the glue tank fixing mechanism 24, but also play a certain clamping role on the glue tank fixing platform 242 when the electrophoresis work is in progress, thereby ensuring that the electrophoresis work can proceed smoothly.

[0034] As described above, in order to ensure the fixing effect of the gel tank fixing platform 242 on the gel plate, the gel tank fixing platform 242 includes a bottom plate, and a fixing platform and an elastic extrusion platform are relatively provided on the side away from the mounting rod 241, and the gel tank can be elastically squeezed between the fixing platform and the elastic extrusion platform.

[0035] In this embodiment, in order to achieve the cooling of the main tank body 21 during the electrophoresis process, the electrophoresis tank also includes an outer tank 1, and the outer tank 1 includes a cooling water tank 11. The main tank body 21 is squatted in the cooling water tank 11, and the plane where the upper end is located is not lower than the plane where the upper end of the cooling water tank 11 is located.

[0036] Furthermore, in order to ensure the cooling effect of the cooling water tank 11, a water inlet pipe 12 and a water outlet pipe 13 are respectively provided on two opposite sides, and the water outlet pipe 13 is arranged close to the upper end of the cooling water tank 11, and the water inlet pipe 12 is opened into the bottom of the cooling water tank 11.

Claims

1. A gene sequencing electrophoresis tank, comprising a main tank body (21) and a cover body (31) on its upper side, wherein opposite sides of the bottom of the main tank body (21) extend downward to form a transition cavity, and a positive electrode terminal (22) and a negative electrode terminal (23) are respectively provided in the two transition cavities, and the cover body (31) is provided with a positive terminal (32) and a negative terminal (33) capable of contacting and communicating with the positive electrode terminal (22) and the negative electrode terminal (23), respectively, and characterized in that: A glue groove fixing mechanism (24) is provided in the middle of the bottom of the main groove body (21); A strip-shaped installation groove is provided in the middle of the bottom of the main tank body (21), and the glue tank fixing mechanism (24) includes a mounting rod (241) and a glue tank fixing platform (242) outside one end thereof, and the end of the mounting rod (241) away from the glue tank fixing platform (242) is hinged to one end inside the installation groove and can be rotated into the installation groove; A driving mechanism (25) capable of driving the glue groove fixing mechanism (24) to flip upward is further provided in the middle of the bottom of the main groove body (21).

2. A gene sequencing electrophoresis tank according to claim 1, characterized in that: The driving mechanism (25) includes a threaded rod (252) arranged inside the installation groove along the installation groove direction, and a wedge block (253) spirally connected to the threaded rod (252) is also slidably provided in the installation groove and is configured to lift the glue groove fixing mechanism (24) by sliding. One end of the threaded rod (252) is also vertically provided with a driving rod (254) whose lower end is transmission-connected thereto.

3. The gene sequencing electrophoresis tank according to claim 2, characterized in that: The upper end of the driving rod (254) is also provided with a knob.

4. The gene sequencing electrophoresis tank according to claim 2, characterized in that: The mounting rod (241) comprises two strips arranged opposite to each other, and the threaded rod (252) and the wedge block (253) are both located between the two strips.

5. The gene sequencing electrophoresis tank according to claim 4, characterized in that: The side of the glue tank fixing platform (242) close to the mounting rod (241) is configured to be able to rotate to be horizontally aligned with the bottom of the main tank body (21).

6. The gene sequencing electrophoresis tank according to claim 4, characterized in that: A convex column (243) is provided on the inner side of one end of the strip away from the glue groove fixing platform (242); The wedge block (253) includes an inclined surface and a vertical surface, and is configured to lift the mounting rod (241) through the cooperation of the inclined surface and the boss (243) when sliding, and to press against one side of the glue groove fixing platform (242) through the vertical surface when sliding in the reverse direction.

7. A gene sequencing electrophoresis tank according to any one of claims 1 to 6, characterized in that: The glue groove fixing platform (242) comprises a bottom plate, and a fixing platform and an elastic extrusion platform are provided on the side of the bottom plate facing away from the installation rod (241).

8. The gene sequencing electrophoresis tank according to any one of claims 1 to 6, characterized in that: It also includes a cooling water tank (11), wherein the main tank body (21) is arranged in the cooling water tank (11), and the plane where the upper end is located is not lower than the plane where the upper end of the cooling water tank (11) is located.

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