Cell culture container

Abstract

The utility model discloses a cell culture container which comprises a containing tank, an intake and exhaust controller, a gas guide assembly and a computer controller, compared with different gas storage tanks in which various gases are installed and stored in the prior art, the burden of equipment cost is reduced, and the computer controller transmits various data back through a detector assembly, so that the cell culture container is convenient to use. By controlling the single mixed gas tank, a computer can conveniently control gas inlet of the gas inlet pump and gas exhaust of the gas exhaust valve so as to realize accurate pressure control in the accommodating tank, and the mixed gas prepared in advance is filled into the gas tank and then filled into the accommodating tank, so that the gas content in the air does not need to be accurately controlled, the manufacturing cost is reduced, and the production efficiency is improved. A high-cost accurate computer does not need to be prepared, and the control cost of the air pressure and the gas content is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture equipment, in particular to a cell culture container. Background Art

[0002] Current cell culture technologies face challenges in simulating specific gas environments, particularly for cells that require high-pressure, low-oxygen conditions. Existing bioreactors typically achieve these conditions by controlling the flow of O2, CO2, and other gases through separate valves, but these devices are often complex and bulky. Utility Model Content

[0003] The purpose of the present invention is to solve the above problems and provide a cell culture container.

[0004] The technical solution of the present utility model is achieved as follows:

[0005] The utility model provides a cell culture container, which comprises:

[0006] a holding tank, wherein the top of the holding tank has an opening;

[0007] an upper cover, detachably mounted on the top of the culture holding tank, for closing or opening the opening;

[0008] An intake and exhaust controller, comprising an intake pump and an exhaust valve mounted on the top of the upper cover, wherein the intake pump is further detachably connected to a mixed gas tank, and the gas in the mixed gas tank can enter the holding tank through the intake pump; and the gas in the holding tank can be discharged through the exhaust valve;

[0009] a gas guide assembly, mounted on the inner wall of the holding tank;

[0010] a detector group, mounted on the inner wall of the holding tank, for detecting data on gas pressure, gas content, and temperature in the holding tank;

[0011] The computer controller is electrically connected to the detector group, the gas guide assembly and the intake and exhaust controller. The computer controller is used to receive data from the detector group and control the intake and exhaust controller and the gas guide assembly to operate.

[0012] The advantages or beneficial effects of the above technical solution include at least:

[0013] Compared with the existing technology of installing different gas storage tanks to store multiple gases, the burden of equipment costs is reduced. The computer controller controls a single mixed gas tank through the various data sent back by the detector component, which facilitates the computer air to control the intake of the intake pump and the exhaust of the exhaust valve to achieve precise pressure control in the holding tank, and the pre-mixed gas is poured into the gas tank and then filled into the holding tank. There is no need to accurately control the gas content in the air, which reduces the production cost, does not require the preparation of high-cost precision computers, and reduces the cost of controlling air pressure and gas content. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The accompanying drawings illustrate exemplary embodiments of the present invention and, together with the description thereof, are used to explain the principles of the present invention. These drawings are included to provide a further understanding of the present invention, and the drawings are included in and constitute a part of this specification.

[0015] Figure 1 A schematic structural diagram of a cell culture container according to an embodiment of the present invention is shown;

[0016] Figure 2 A schematic diagram of airflow in a storage tank according to an embodiment of the present invention is shown;

[0017] Figure 3 A schematic top view of the airflow in the container in the storage state of an embodiment of the present invention is shown;

[0018] Figure 4 A schematic diagram showing the inclination angles of the first fan and the second fan in an embodiment of the present utility model;

[0019] Figure 5 A schematic diagram of the air flow in the container when the air intake pump is taking in air and the exhaust valve is exhausting air is shown in the embodiment of the utility model;

[0020] Figure 6 A schematic diagram of the installation of the upper cover and the containing tank according to an embodiment of the utility model is shown;

[0021] Figure 7 A schematic diagram showing the position of the chute in an embodiment of the utility model is shown;

[0022] Figure 8 A schematic diagram of a detector group according to an embodiment of the present invention is shown;

[0023] Figure 9 A schematic diagram of the position of the electric heating wire and a cross-sectional schematic diagram of the support portion on the mounting base are shown in an embodiment of the present utility model.

[0024] Figure numerals: 10, containing tank; 11, first fan; 12, second fan; 13, first diameter dividing line; 14, second diameter dividing line; 15, fixing rod; 16, thickened portion; 161, inclined surface; 17, annular groove; 18, bolt; 20, upper cover; 21, inclined groove; 22, annular protrusion; 30, intake and exhaust controller; 31, intake pump; 32, exhaust valve; 40, detector group; 41, mounting seat; 411, support portion; 42, detection probe; 50, computer controller; 60, mixed gas tank; 70, electric heating wire. DETAILED DESCRIPTION

[0025] The following describes embodiments of the present invention in more detail with reference to the accompanying drawings. Although certain embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be construed as limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and complete understanding of the present invention. It should be understood that the accompanying drawings and embodiments of the present invention are for illustrative purposes only and are not intended to limit the scope of protection of the present invention.

[0026] It should be noted that, in the absence of conflict, the embodiments and features of the embodiments of the present invention can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0027] It should be understood that the term "including" and its variations used in this document are open inclusions, that is, "including but not limited to". The term "based on" means "based at least in part on". The term "one embodiment" means "at least one embodiment"; the term "another embodiment" means "at least one other embodiment"; the term "some embodiments" means "at least some embodiments". The relevant definitions of other terms will be given in the following description. It should be noted that the concepts of "first", "second", etc. mentioned in this utility model are only used to distinguish different devices, modules or units, and are not used to limit the order or interdependence of the functions performed by these devices, modules or units.

[0028] It should be noted that the modifications of "one" and "multiple" mentioned in the present invention are illustrative rather than restrictive. Those skilled in the art should understand that unless otherwise clearly indicated in the context, it should be understood as "one or more".

[0029] The names of the messages or information exchanged between multiple devices in the embodiments of the present invention are only used for illustrative purposes and are not used to limit the scope of these messages or information.

[0030] A cell culture container, comprising the following components:

[0031] The holding tank 10 is a cylindrical structure. The cylindrical structure of the holding tank 10 can reduce welds and improve sealing. There is an opening at the top of the holding tank 10, through which the user can place the cell culture dish into the holding tank 10; the top of the holding tank 10 can also be detachably installed with an upper cover 20 for closing or opening the opening; specifically, a fixing rod 15 is fixedly connected to the base of the holding tank 10, and the end of the fixing rod 15 away from the base is detachably connected to the upper cover 20 by a bolt 18, so that the upper cover 20 can be tightly installed above the holding tank 10 to prevent air loss in the holding tank 10. At the same time, the bolt 18 can be used to tighten the upper cover 20. 18 is removed so that the upper cover 20 can be removed from the upper end of the holding tank 10 to access the culture holding tank 10 in the holding tank 10. As a further improvement, a thickened portion 16 extending outward is provided at the opening of the holding tank 10, and the thickened portion 16 has an outwardly inclined inclined surface 161, and the bottom of the upper cover 20 has an inclined groove 21 adapted to the inclined surface 161 to increase the contact area between the upper cover 20 and the holding tank 10, and further improve the sealing between the upper cover 20 and the holding tank 10. An annular groove 17 is provided between the thickened portion 16 and the inner wall of the holding tank 10, and the bottom of the upper cover 20 has an annular protrusion 22 that fits with the annular groove 17.

[0032] The intake and exhaust controller 30 includes an intake pump 31 and an exhaust valve 32 installed on the top of the upper cover 20. The intake pump 31 can also be detachably connected to a mixed gas tank 60, wherein the mixed gas tank 60 contains a mixed gas, which is a mixed gas of 2% O2, 5% CO2 and 93% N. The gas in the mixed gas tank 60 can enter the containing tank 10 through the intake pump 31. When the mixed gas is filled into the containing tank 10, the mixed gas will increase the air pressure in the containing pipe to form a pressure chamber; in addition, the gas in the containing tank 10 can be discharged through the exhaust valve 32.

[0033] At the same time, a HEPA filter system is installed between the air intake pump 31 and the gas tank. The mixed gas passes through the HEPA filter system to filter out impurities and bacteria in the gas, creating a relatively sterile environment in the holding tank 10;

[0034] The gas guide assembly is installed on the inner wall of the container 10. The gas guide assembly specifically includes the following components: a first fan 11 and a second fan 12; Figure 2 As shown, the projections of the first fan 11 and the second fan 12 in the vertical direction have a vertical height difference, wherein the height of the first fan 11 is greater than that of the second fan; the projections of the first fan 11 and the second fan 12 in the horizontal direction are both on the same side of the first diameter dividing line 13 of the holding tank 10, and both are installed on the inner walls on both sides of the second diameter dividing line 14 of the holding tank 10, as shown in FIG. Figure 3As shown, the first fan 11 will blow the air at the top of the container 10 downward, and the air will flow downward along the inner wall of the cylindrical container 10. At this time, the second fan 12 located below will continue to blow the drawn air to the bottom of the container 10, so that the air forms a vortex in the container 10. Figure 2 , increasing the air flow in the holding tank 10, so that the air mixing degree in the holding tank 10 is increased; wherein the first diameter dividing line 13 and the second diameter dividing line 14 are perpendicular to each other; and the first fan 11 and the second fan 12 are both inclined downward by 45 degrees;

[0035] The culture container further includes: a detector group 40 installed on the inner wall of the holding tank 10 for detecting data such as air pressure, gas content and temperature in the holding tank 10; and an electric heating wire 70 installed at the bottom of the holding tank 10 for heating the air in the holding tank 10.

[0036] The culture container further includes: a computer controller 50, which is electrically connected to the detector group 40, the gas guide assembly and the intake and exhaust controller 30. The culture container further includes: an electric heating wire 70, which is installed at the bottom of the holding tank 10; the computer controller 50 is also electrically connected to the electric heating wire 70 installed at the bottom of the holding tank 10, and the computer controller 50 further controls the operation of the electric heating wire 70.

[0037] The computer controller 50 is used to receive data from the detector group 40, such as the air composition, air pressure, temperature and other data in the holding tank 10. The computer controller 50 controls the air intake and exhaust controller 30 and the gas guide component to operate according to the above data. Specifically, when the mixed gas in the mixed gas tank 60 is filled into the holding tank 10, the concentration and pressure of O2 and CO2 in the holding tank 10 are changed. At the same time, the detector group 40 senses O2 and CO2 and uploads them to the computer controller 50. Similarly, before the computer control controls the electric heating wire 70 to heat up, the detector group 40 uploads the temperature signal to the computer controller 50. After information processing, the computer controller 50 adjusts the air intake pump 31 and the electric heating wire 70 to dynamically balance the environment in the holding tank 10, thereby creating suitable conditions for cell culture at the bottom of the holding tank 10.

[0038] Based on the above structure, when the computer controller 50 controls the air intake pump 31 and the exhaust valve 32 to be closed, the computer controller 50 controls the first fan 11 and the second fan 12 to blow air downwards. Figure 2 As shown, the air in the holding tank 10 is made to flow and the gas in the holding tank 10 is evenly distributed. When the computer controller 50 controls the air intake pump 31 to intake air and controls the exhaust valve 32 to exhaust air, as shown Figure 5As shown, the first fan 11 blows air upward to assist the air in the holding tank 10 to be blown out from the exhaust hole of the exhaust valve 32, and the second fan 12 blows air downward to assist the mixed gas entering from the intake pump 31 to flow from the top to the bottom of the holding tank 10, so that the mixed gas is quickly and evenly distributed in the holding tank 10.

[0039] Based on the above structure, the detector assembly 40 includes: a mounting base 41, mounted on the side wall of the holding tank 10, with one side flush with the inner wall of the holding tank 10 and the other side extending outside the holding tank 10, the side extending outside the holding tank 10 being connected to the computer controller 50 via a wire; a detection probe 42, mounted on the side of the mounting base 41 extending into the holding tank 10, so that the detection probe 42 can detect the inside of the holding tank 10. The detection probe 42 includes a temperature sensor, an air pressure sensor, a gas content sensor, etc., and is used to detect various data within the holding tank 10;

[0040] Further improvement based on the above structure: a support portion 411 is extended from one side of the mounting seat 41 close to the detection probe 42, and a groove corresponding to the extended support portion 411 is opened on the inner wall of the storage tank 10 to increase the contact area between the support portion 411 and the side wall of the storage tank 10, so that the mounting seat 41 is more stable on the side wall of the storage tank 10 and not easy to fall.

[0041] Many studies have attempted to simulate mechanical stress through bioreactors. The key is how to transfer pressure to cartilage. The earliest bioreactors used direct contact pressurization (compression load), but this method easily causes cartilage loss. In contrast, hydrostatic pressure can produce more matrix (such as GAG, collagen I, II) and improve mechanical properties. Later, it developed into pressurization by squeezing liquid (pressure range of about 5-10MPa), but this is different from the hydrostatic pressure (about 5MPa) when the human knee joint is standing. In recent years, the method of gas pressurization (pressure range of about 10-100kPa) has been used to obtain results that show that micro-hydrostatic pressure can promote cartilage regeneration, which is more in line with clinical research. However, previous bioreactors did not consider the problem of hypoxia culture. The oxygen partial pressure in the human knee joint cavity is 50±9mmHg, while the oxygen partial pressure in an ordinary incubator is 140mmHg, which far exceeds the oxygen partial pressure in the joint cavity. The cartilage tissue culture device of this application achieves hypoxia conditions by gas pressurization; current cell culture technology has challenges in simulating the needs of specific gas environments, especially for cell culture that requires high pressure and low oxygen conditions. Existing bioreactors usually achieve culture conditions by controlling multiple gas valves to control the entry of O2, CO2 and other gases respectively, but these devices are often too complicated and bulky. The present application mixes the gases in multiple gas tanks into one gas tank, and then controls the gas valves to allow the mixed gas in the mixed gas tank to be filled into the cell culture container of the present application, thereby reducing the burden on the equipment. In addition, the computer controller 50 controls a single mixed gas tank through the various data transmitted back by the detector group 40, which facilitates the computer air to control the intake of the intake pump 31 and the exhaust of the exhaust valve 32, so as to achieve precise pressure control in the holding tank 10.

[0042] In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they cannot be understood as limitations on the present invention.

[0043] Those skilled in the art will appreciate that the above embodiments are merely for the purpose of illustrating the present invention and are not intended to limit the scope of the present invention. Other variations or modifications may be made based on the above disclosure, and such variations or modifications are still within the scope of the present invention.

Claims

1. A cell culture container, characterized in that: The culture container comprises: A holding tank (10), wherein the top of the holding tank (10) has an opening, and an upper cover (20) is detachably mounted on the upper end of the holding tank (10), and the upper cover (20) is used to close or open the opening; An intake and exhaust controller (30) comprises an intake pump (31) and an exhaust valve (32) mounted on the top of the upper cover (20); the intake pump (31) is further detachably connected to a mixed gas tank (60); gas in the mixed gas tank (60) can enter the holding tank (10) through the intake pump (31); and gas in the holding tank (10) can be discharged through the exhaust valve (32); A gas guide assembly is mounted on the inner wall of the holding tank (10); a detector group (40), mounted on the inner wall of the holding tank (10), for detecting data on gas pressure, gas content, and temperature within the holding tank (10); A computer controller (50) is electrically connected to the detector group (40), the gas guide assembly and the intake and exhaust controller (30). The computer controller (50) is used to receive data from the detector group (40) and control the intake and exhaust controller (30) and the gas guide assembly to operate.

2. The cell culture container according to claim 1, wherein: The gas guide assembly includes a first fan (11) and a second fan (12); The projections of the first fan (11) and the second fan (12) in the vertical direction have a vertical height difference; The horizontal projections of the first fan (11) and the second fan (12) are both located on the same side of a first diameter dividing line (13) of the holding tank (10), and the two are installed on the inner walls on both sides of a second diameter dividing line (14) of the holding tank (10); The first diameter dividing line (13) and the second diameter dividing line (14) are perpendicular to each other; The first fan (11) and the second fan (12) are both tilted downward by 45 degrees; When the computer controller (50) controls the air intake pump (31) and the exhaust valve (32) to be closed, the computer controller (50) controls the first fan (11) and the second fan (12) to blow air downward; When the computer controller (50) controls the air intake pump (31) to intake air and controls the air exhaust valve (32) to exhaust air, the first fan (11) blows air upwards and the second fan (12) blows air downwards.

3. The cell culture container according to claim 2, wherein: The detector group (40) includes: A mounting seat (41) is mounted on the side wall of the holding tank (10), with one side flush with the inner wall of the holding tank (10) and the other side extending out of the holding tank (10); A detection probe (42) is mounted on a side of the mounting seat (41) extending into the holding tank (10), so that the detection probe (42) can detect inside the holding tank (10).

4. The cell culture container according to claim 3, wherein: A support portion (411) extends from one side of the mounting seat (41) close to the detection probe (42), and a groove corresponding to the support portion (411) is formed on the inner wall of the holding tank (10).

5. The cell culture container according to claim 4, characterized in that: A fixing rod (15) is fixedly connected to the base of the holding tank (10), and one end of the fixing rod (15) away from the base is detachably connected to the upper cover (20) via a bolt (18).

6. The cell culture container according to claim 5, characterized in that: The opening of the holding tank (10) is provided with a thickened portion (16) extending outward, the thickened portion (16) is provided with an outwardly inclined inclined surface (161), and the bottom of the upper cover (20) is provided with an inclined groove (21) adapted to the inclined surface (161).

7. The cell culture container according to claim 6, wherein: An annular groove (17) is provided between the thickened portion (16) and the inner wall of the holding tank (10), and the bottom of the upper cover (20) has an annular protrusion (22) that fits with the annular groove (17).

8. The cell culture container according to claim 5, wherein: The culture container further comprises: an electric heating wire (70), the electric heating wire (70) being installed at the bottom of the holding tank (10); The computer controller (50) further controls the operation of the electric heating wire (70).

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