Cell culture device

Abstract

The utility model provides a cell culture device. The cell culture device comprises an upper cover and a base which are matched with each other, the upper cover and the base are matched to form a cell culture space; the plurality of first through holes are circumferentially formed in the side wall of the base by taking the central axis of the base as an axis, and are communicated with the cell culture space; the sealing device is detachably connected to the upper cover and can slide up and down along the base; wherein the sealing device moves to a first position relative to the upper cover, the sealing device shields any one of the first through holes, and the cell culture space is closed; the sealing device moves from a first position to a second position relative to the upper cover, at least one first through hole is exposed, and the cell culture space is communicated with the outside; the utility model solves the technical problems that the universality of a cell culture device in the related technology is not strong, and particularly, when different types of experiments are carried out in a laboratory, a sealed culture device and a non-sealed culture device are required to be prepared respectively, so that the experiment cost is increased, and more storage space is occupied.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture device. Background Art

[0002] With the continuous deepening of research on cell structure, function, metabolism, signal transduction, etc., a tool that can conveniently and efficiently culture cells is needed. Cell culture devices have emerged as a result. Cell culture devices can provide a certain space for cells to grow and reproduce in vitro, providing an important experimental platform for cell biology research.

[0003] However, there is at least one of the following problems in the related technology: the cell culture devices in the related technology are not very versatile. In particular, when laboratories conduct different types of experiments, they often need to prepare sealed culture devices and non-sealed culture devices separately, which not only increases the experimental cost, but also takes up more storage space. Utility Model Content

[0004] The technical problem solved by the present invention is that the cell culture devices in the related art are not very versatile. In particular, when laboratories conduct different types of experiments, they often need to prepare sealed culture devices and non-sealed culture devices separately, which not only increases the experimental costs but also takes up more storage space.

[0005] To solve the above problems, the present invention provides a cell culture device, comprising: an upper cover and a base arranged in cooperation with each other; the upper cover and the base cooperate to form a cell culture space; a plurality of first through holes, the plurality of first through holes being circumferentially arranged on the side wall of the base with the central axis of the base as the axis, and being connected to the cell culture space; a sealing device, the sealing device being detachably connected to the upper cover and being able to slide up and down along the base; wherein, when the sealing device moves to a first position relative to the upper cover, the sealing device blocks any one of the plurality of first through holes, thereby closing the cell culture space; when the sealing device moves from the first position to the second position relative to the upper cover, at least one first through hole is exposed, and the cell culture space is connected to the outside world.

[0006] Compared with the existing technology, the technical effect achieved by adopting this technical solution is: unlike the related technology where the cell culture devices are mostly single-type culture plates and cannot achieve the switching of the closed and ventilated states of their cell culture space, the utility model provides a sealing device that can slide up and down along the base, and combines a plurality of first through holes arranged on the base that are connected to the cell culture space, and uses the sealing device to achieve the switching of the closed and external communication of the cell culture space, providing a controllable environment for cell culture, and the sealing device and the upper cover are detachably connected. When the sealing device moves in the direction of the first position, the sealing device detaches from the upper cover and slides along the base until it moves to the first through hole, closing the cell culture space; in contrast, when the sealing device moves from the first position to the second position, the cell culture space is connected to the outside world and is engaged with the upper cover to reduce the overall volume of the entire cell culture device.

[0007] In one embodiment of the present invention, the sealing device includes: a sealing member, which is sleeved on the outer side of the base; and a clamping member, which is provided on a side of the sealing member close to the upper cover; wherein the clamping member is clamped with the upper cover.

[0008] Compared with the prior art, the technical effect achieved by adopting this technical solution is that the sealing device and the upper cover are detachably connected by providing a snap-fit ​​piece, making it convenient for the user to disassemble the sealing device.

[0009] In one example of the present invention, a first guide surface is provided at the position of the upper cover corresponding to the clamping member; a first guide member is provided on the side of the clamping member away from the sealing member; wherein, when the first guide member moves along the first guide surface to the second position, the first guide member is clamped with the upper cover to fix the sealing device to the upper cover.

[0010] Compared with the existing technology, the technical effect achieved by adopting this technical solution is as follows: a first guide surface is provided on the upper cover, and a first guide member is provided on the clamping member, so that the sealing device can reach the clamping position more smoothly during the movement process.

[0011] In one embodiment of the present invention, a mating cavity is provided at a position of the upper cover corresponding to the snap-fit ​​part; the cell culture device further comprises an adjusting part; the adjusting part is movably arranged in the mating cavity and is used to adjust the snap-fit ​​position of the snap-fit ​​part so that the snap-fit ​​part is disengaged from the mating cavity.

[0012] Compared with the existing technology, the technical effect achieved by adopting this technical solution is: by providing an adjustment piece for adjusting the clamping position of the clamping piece, the clamping piece can be more conveniently disengaged from the matching cavity, thereby increasing the flexibility of operation.

[0013] In one example of the present invention, the adjusting member includes: a pressing member and a second guide member arranged opposite to each other; the second guide member is arranged in the matching cavity; the pressing member is located on the side of the second guide member away from the matching cavity; wherein, pressing the pressing member causes the second guide member to squeeze the first guide member to disengage it from the matching cavity.

[0014] Compared with the existing technology, the technical effect achieved by adopting this technical solution is that the card connector is disengaged from the mating cavity by pressing, which is more convenient for users to operate.

[0015] In one example of the present invention, the sealing device further includes a plurality of blocking members; the blocking members are arranged on a side of the sealing member close to the base and corresponding to the first through hole; wherein, when the sealing member is located in the first position, the blocking members block the first through hole.

[0016] Compared with the prior art, the technical effect achieved by adopting this technical solution is that a plurality of blocking members corresponding to the first through holes are provided to block the first through holes, so that the cell culture space is closed.

[0017] In one example of the present invention, the blocking member includes: a blocking plug, which is used to block the first through hole; a second elastic member, one end of the second elastic member is connected to the blocking plug, and the other end is connected to the sealing member; wherein, when the sealing device is in the first position, the second elastic member pushes the blocking plug to block the first through hole.

[0018] Compared with the existing technology, the technical effect achieved by adopting this technical solution is: by setting a blocking plug and a second elastic member, when the sealing device is in the first position, the second elastic member pushes the blocking plug to block the first through hole, thereby realizing the function of automatic sealing and improving convenience.

[0019] In one embodiment of the present invention, the base includes a plurality of placement positions; the placement positions are located on a side of the base close to the upper cover and are used to place cell culture dishes.

[0020] Compared with the existing technology, the technical effect achieved by adopting this technical solution is as follows: a placement position specifically for placing cell culture dishes is provided to improve the stability of the cell culture dishes in the cell culture device.

[0021] In one embodiment of the present invention, it further comprises: a viewing window, which is provided on the upper cover and is used to observe the cell culture conditions in the cell culture space.

[0022] Compared with the existing technology, the technical effect achieved by adopting this technical solution is that the visual window is provided to facilitate users to observe the cell culture conditions in the cell culture space.

[0023] In one embodiment of the present invention, the utility model further comprises: a shielding member movably provided on the upper cover for shielding the visual window or leaving the visual window exposed.

[0024] Compared with the existing technology, the technical effect achieved by adopting this technical solution is that the shielding member can be set to block or expose the visual window as needed, further protecting the cell culture space from external interference.

[0025] After adopting the technical solution of the utility model, the following technical effects can be achieved:

[0026] (1) By providing a sealing device that can slide up and down along the base, and combining a plurality of first through holes provided on the base that are connected to the cell culture space, the sealing device is used to switch between sealing the cell culture space and connecting it to the outside world, thereby providing a controllable environment for cell culture;

[0027] (2) A sealing device detachably connected to the upper cover is provided to reduce the volume of the entire cell culture device;

[0028] (3) A blocking plug and a second elastic member are provided. When the sealing device is in the first position, the second elastic member can push the blocking plug to block the first through hole, thereby realizing the automatic sealing function and improving the overall convenience;

[0029] (4) A visual window and a shielding member are provided, and the visual window can be covered or exposed according to actual needs to facilitate users to observe the cell culture conditions in the cell culture space. It is no longer necessary to open the upper cover to observe the cell culture dish, and the cell culture dish in the cell culture space is further protected from external interference. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings to be used in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. Those skilled in the art can also derive other drawings based on these drawings without inventive efforts.

[0031] Figure 1 An exploded view of a cell culture device provided in an embodiment of the present utility model;

[0032] Figure 2 A top view of a cell culture device provided in an embodiment of the present utility model;

[0033] Figure 3 for Figure 2 Cross-sectional view along AA direction;

[0034] Figure 4 for Figure 3 A partial enlarged view of point B in the middle;

[0035] Figure 5 is a cross-sectional view when the sealing device is in a first position;

[0036] Figure 6 for Figure 5 A partial enlarged view of point C in the middle.

[0037] Description of reference numerals:

[0038] 100. Cell culture device; 10. Upper cover; 11. Matching cavity; 20. Base; 21. First through hole; 22. Placement position; 23. Side wall; 30. Sealing device; 31. Sealing member; 32. Snap-fit ​​member; 321. First guide member; 322. First elastic member; 33. Blocking member; 331. Shielding plug; 332. Second elastic member; 40. Adjusting member; 41. Pressing member; 42. Second guide member. DETAILED DESCRIPTION

[0039] The following describes in detail embodiments of the present invention. Examples of the embodiments are shown in the accompanying drawings, in which the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention and are not to be construed as limiting the present invention. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0040] In the description of this utility model, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they may refer to fixed connection, connection, or integral connection; they may refer to mechanical connection or electrical connection; they may refer to direct connection or indirect connection through an intermediate medium; and they may refer to internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.

[0041] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, specific embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0042] See also Figure 1 , Figure 1 An exploded diagram of a cell culture device provided in an embodiment of the present invention; Figures 2 to 6Specifically, the present invention provides a cell culture device 100, comprising: an upper cover 10 and a base 20, a first through hole 21 and a sealing device 30 that are arranged in cooperation with each other; wherein the upper cover 10 and the base 20 cooperate to form a cell culture space; there are multiple first through holes 21, and the multiple first through holes 21 are circumferentially arranged on the side wall 23 of the base 20 with the central axis of the base 20 as the axis, and are connected to the cell culture space; the sealing device 30 is detachably connected to the upper cover 10 and can slide up and down along the base 20; wherein, when the sealing device 30 moves to the first position relative to the upper cover 10, the sealing device 30 blocks any one of the multiple first through holes 21, so that the cell culture space is closed; when the sealing device 30 moves from the first position to the second position relative to the upper cover 10, at least one first through hole 21 is exposed, so that the cell culture space is connected to the outside.

[0043] Optionally, the base 20 includes a side wall 23 , an outer surface of the side wall 23 close to the upper cover 10 is provided with an internal thread, and the upper cover 10 is correspondingly provided with an external thread, so that the upper cover 10 and the base 20 are threadedly connected.

[0044] Furthermore, the sealing device 30 includes: a sealing member 31 and a snap-fit ​​member 32; wherein, the sealing member 31 is sleeved on the base 20 and can slide up and down along the base 20, and the snap-fit ​​member 32 is arranged on the side of the sealing member 31 close to the upper cover 10; the snap-fit ​​member 32 is snap-fitted to the upper cover 10.

[0045] Furthermore, a first guide surface is provided at the position of the upper cover 10 corresponding to the clamping member 32; a first guide member 321 is provided on the side of the clamping member 32 away from the sealing member 31; wherein, when the first guide member 321 moves along the first guide surface to the second position, the first guide member 321 is clamped with the upper cover 10, so that the sealing member 31 is fixed to the upper cover 10.

[0046] Furthermore, a mating cavity 11 is provided at the position of the upper cover 10 corresponding to the snap-fit ​​part 32 ; the cell culture device 100 also includes an adjusting part 40 ; the adjusting part 40 is movably provided in the mating cavity 11 for adjusting the snap-fit ​​position of the snap-fit ​​part 32 so that the snap-fit ​​part 32 is disengaged from the mating cavity 11 .

[0047] Furthermore, the clamping member 32 also includes a first elastic member 322, which is arranged on the side of the first guide member 321 close to the second guide member 42; wherein, when the sealing member 31 moves from the first position to the second position, the first elastic member 322 changes from a normal state to a compressed state until the sealing member 31 moves to the second position, and the first elastic member 322 restores its deformation and changes to a normal state.

[0048] Furthermore, the adjusting member 40 includes: a pressing member 41 and a second guide member 42 arranged opposite to each other; the second guide member 42 is arranged in the matching cavity 11; the pressing member 41 is located on the side of the second guide member 42 away from the matching cavity 11; wherein, by pressing the pressing member 41, the second guide member 42 squeezes the first guide member 321 so that it detaches from the matching cavity 11 along the first guide surface.

[0049] See also Figure 3 and Figure 4 , Figure 3 It is a cross-sectional view of the sealing device 30 when it is in the second position; specifically, the sealing member 31 is sleeved on the base 20 and can move up and down along the base 20; wherein, when the sealing member 31 moves in the direction close to the upper cover 10, until the first guide member 321 of the clamping member 32 moves along the first guide surface into the matching cavity 11, and the first guide member 321 is clamped with the upper cover 10, the sealing device 30 is in the second position at this time, and the cell culture space formed by the upper cover 10 and the base 20 is connected to the outside world through the first through hole 21; in contrast, when it is necessary to detach the clamping member 32 from the upper cover 10, by pressing the pressing member 41, the second guide member 42 squeezes the first guide member 321 to compress it, and at the same time moves the sealing member 31 in the direction of the first position, so that the clamping member 32 is detached from the matching cavity 11, which is more convenient for user operation.

[0050] For further information, see Figure 5 and Figure 6 The sealing device 30 also includes a plurality of blocking members 33 ; the blocking members 33 are arranged on a side of the sealing member 31 close to the base 20 and are arranged corresponding to the first through hole 21 ; wherein, when the sealing member 31 is in the first position, the blocking member 33 blocks the first through hole 21 .

[0051] Specifically, the blocking member 33 includes: a blocking plug 331 and a second elastic member 332; the blocking plug 331 is used to block the first through hole 21; one end of the second elastic member 332 is connected to the blocking plug 331, and the other end is connected to the sealing member 31; wherein, when the sealing device 30 is in the first position, the second elastic member 332 pushes the blocking plug 331 to block the first through hole 21.

[0052] In combination with specific usage, when the sealing member 31 is in the second position, the blocking member 33 is in a compressed state; when the sealing member 31 is in the first position, the blocking member 33 is aligned with the first through hole 21. At this time, the second elastic member 332 recovers its deformation from the original compressed state and pushes the blocking plug 331 to move in the direction of the first through hole 21 to block the first through hole 21. At this time, the cell culture space formed by the upper cover 10 and the base 20 is blocked by the blocking plug 331, and the cell culture space is closed.

[0053] Preferably, the shape and size of the blocking plug 331 are adapted to the first through hole 21 .

[0054] Preferably, the base 20 includes a plurality of placement positions 22 ; the placement positions 22 are located on a side of the base 20 close to the upper cover 10 and are used to place cell culture dishes.

[0055] Preferably, the cell culture device 100 further includes a viewing window provided on the upper cover 10 for observing the cell culture conditions in the cell culture space.

[0056] Preferably, the cell culture device 100 further includes: a shielding member, which is movably provided on the upper cover 10 and is used to shield the visual window or expose the visual window; specifically, the visual window and the shielding member are both located in the upper cover 10, the visual window is located on the side of the upper cover 10 close to the base 20, and the shielding member is located on the side of the visual window away from the base 20, and the visual window is exposed or shielded by rotating the shielding member.

[0057] In combination with specific usage conditions, during the cell culture process, the user can observe the growth status, morphological changes, etc. of the cells at any time through the visual window of the upper cover 10 without having to remove the upper cover 10 from the base 20, thereby reducing interference with the cell culture environment; and when the light in the laboratory is strong, the user can rotate the shielding member to put the visual window in a shielded state, thereby protecting the cells in the cell culture space from interference from strong light exposure, effectively blocking external light, and ensuring that the cells grow in a suitable environment.

[0058] Although the present invention is disclosed as above, it is not limited thereto. Any person skilled in the art may make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention shall be subject to the scope defined by the claims.

Claims

1. A cell culture device, characterized in that: include: An upper cover (10) and a base (20) are arranged in cooperation with each other; the upper cover (10) and the base (20) cooperate to form a cell culture space; a plurality of first through holes (21), the plurality of first through holes (21) being circumferentially arranged on the side wall (23) of the base (20) with the central axis of the base (20) as an axis, and being in communication with the cell culture space; a sealing device (30), the sealing device (30) being detachably connected to the upper cover (10) and being slidable up and down along the base (20); When the sealing device (30) moves to a first position relative to the upper cover (10), the sealing device (30) blocks any one of the plurality of first through holes (21), thereby sealing the cell culture space; When the sealing device (30) moves from the first position to the second position relative to the upper cover (10), at least one of the first through holes (21) is exposed, and the cell culture space is connected to the outside.

2. The cell culture device according to claim 1, characterized in that The sealing device (30) comprises: A sealing member (31), wherein the sealing member (31) is sleeved on the outer side of the base (20); A clamping member (32), the clamping member (32) being provided on a side of the sealing member (31) close to the upper cover (10); Wherein, the clamping member (32) is clamped to the upper cover (10).

3. The cell culture device according to claim 2, characterized in that The upper cover (10) is provided with a first guide surface at a position corresponding to the clamping member (32); A first guide member (321) is provided on a side of the clamping member (32) away from the sealing member (31); When the first guide member (321) moves along the first guide surface to the second position, the first guide member (321) is engaged with the upper cover (10), so that the sealing device (30) is fixed to the upper cover (10).

4. The cell culture device according to claim 3, characterized in that The upper cover (10) is provided with a matching cavity (11) at a position corresponding to the clamping member (32); The cell culture device further includes a regulating member (40); The adjusting member (40) is movably disposed in the matching cavity (11) and is used to adjust the clamping position of the clamping member (32) so that the clamping member (32) is disengaged from the matching cavity (11).

5. The cell culture device according to claim 4, characterized in that The adjusting member (40) comprises: a pressing member (41) and a second guiding member (42) arranged opposite to each other; The second guide member (42) is provided in the matching cavity (11); The pressing member (41) is located on a side of the second guide member (42) away from the fitting cavity (11); The pressing member (41) is pressed to cause the second guide member (42) to squeeze the first guide member (321) so that the first guide member (321) is disengaged from the fitting cavity (11).

6. The cell culture device according to claim 2, characterized in that The sealing device (30) further includes a plurality of sealing members (33); The blocking member (33) is provided on a side of the sealing member (31) close to the base (20) and is provided corresponding to the first through hole (21); Wherein, when the sealing member (31) is located at the first position, the blocking member (33) blocks the first through hole (21).

7. The cell culture device according to claim 6, characterized in that The blocking member (33) comprises: a blocking plug (331), the blocking plug (331) being used to block the first through hole (21); a second elastic member (332), one end of the second elastic member (332) being connected to the blocking plug (331) and the other end being connected to the sealing member (31); Wherein, when the sealing device (30) is located at the first position, the second elastic member (332) pushes the blocking plug (331) to block the first through hole (21).

8. The cell culture device according to claim 1, wherein The base (20) includes a plurality of placement positions (22); The placement position (22) is located on a side of the base (20) close to the upper cover (10) and is used for placing a cell culture dish.

9. The cell culture device according to claim 1, wherein Also includes: A visual window is provided on the upper cover (10) and is used for observing the cell culture conditions in the cell culture space.

10. The cell culture device according to claim 9, characterized in that Also includes: A shielding member is movably provided on the upper cover (10) and is used for shielding the visual window or leaving the visual window exposed.

Images