Cell cryopreservation tube
The double sealing structure and water absorption ring design solve the problem of insufficient sealing of cell freezing tubes, achieve higher sealing and waterproof effects, and avoid cell contamination.
Patent Information
- Application Number
- CN202422863144.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-23
- Publication Date
- 2025-09-23
- Estimated Expiration
- 2034-11-23
AI Technical Summary
Existing cell cryopreservation tubes are not sufficiently sealed, which allows liquid nitrogen or water to easily enter the cryopreservation tubes, causing cell contamination or damage.
The double sealing structure, including threaded connection and sliding buckle controlled sealing components, combined with the water absorption ring design, ensures the sealing and waterproof effect of the cryopreservation tube.
It effectively prevents liquid nitrogen or water from entering the cryopreservation tube, avoids cell contamination, and improves the sealing and practicality of the cryopreservation tube.
Smart Images
Figure CN223364881U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of experimental instruments, in particular to a cell freezing tube. Background Art
[0002] Cell cryopreservation is a technology that preserves cells in an ultra-low temperature environment, usually using liquid nitrogen (-196°C) for long-term storage. Under such low temperature conditions, the metabolic activity of the cells almost completely stops, thereby preserving the biological characteristics of the cells for a long time. Cell recovery refers to the process of thawing and re-culturing frozen cells under suitable conditions. During recovery, cells need to be thawed quickly to avoid damage to the cells caused by the recrystallization of ice crystals. Usually, cells are thawed in a 37°C water bath, and if necessary, they need to be shaken in water to melt as quickly as possible. In this process, due to the large temperature difference between freezing and recovery, water vapor will be generated in the test tube. Sometimes liquid nitrogen or water will flow into the cell cryopreservation tube from between the lid and the tank body. The generation of water vapor may have a certain impact on cell recovery. If liquid nitrogen or water flows into the tube from between the lid and the tube body, it is easy to contaminate the sample in the cell cryopreservation tube, and even cause cell damage or death. In the prior art, the lid of the cryopreservation tube is usually mainly connected to the tube body by a thread, but its sealing is not strong enough, and water will enter during actual use. Utility Model Content
[0003] The purpose of the present invention is to provide a cell freezing tube in response to the above problems.
[0004] The technical solution adopted by the present invention to solve the above technical problems is:
[0005] A cell freezing tube comprises a tube body and a tube cover that covers the tube body, the tube cover comprising an outer cover body and an inner cover body, the inner diameter of the outer cover body being larger than the outer diameter of the open end of the tube body, and the outer diameter of the inner cover body being smaller than the inner diameter of the open end of the tube body; an inner cavity channel is provided inside the inner cover body, the inner cavity channel comprising a horizontal channel and a vertical channel that are connected to each other, a sealing assembly is movably connected in the horizontal channel, the sealing assembly comprises a sealing ring and a sealing rod, one end of the sealing rod is fixedly connected to the sealing ring, a control rod is provided in the vertical channel, one end of the control rod is movably connected to the inner cover body through an elastic member, and a reducing portion is provided on the middle outer circumferential surface of the control rod; a sliding buckle is provided on the top of the tube cover, the sliding buckle is slidably connected to the tube cover, and moving the sliding buckle can make the bottom end of the sliding buckle abut against or separate from the other end of the control rod.
[0006] As a preferred solution, a sliding groove adapted to the sliding buckle is provided on the top of the tube cover, and the sliding buckle is arranged in the sliding groove.
[0007] As a preferred solution, a convex ring is provided on the inner circumferential surface of the outer cover, and the convex ring is arranged on the same horizontal plane as the sealing ring.
[0008] As a preferred solution, the inner circumferential surface of the outer cover is provided with a second thread, and the outer circumferential surface of the tube body is provided with a first thread that matches the second thread.
[0009] As a preferred solution, the inner cover and the sealing ring are both made of rubber material.
[0010] As a preferred solution, an annular groove is provided on the top of the tube body, and a water absorption ring is detachably embedded in the annular groove.
[0011] As a preferred solution, the water absorption ring is made of highly water-absorbent material.
[0012] Due to the adoption of the above technical solution, the utility model has the following beneficial effects:
[0013] 1. The tube body and the tube cover are tightly connected by threads, and the sealing assembly is driven by the slide buckle and the control rod, so that the sealing ring presses the side wall of the inner cover body and then presses against the inner side of the tube body, and cooperates with the convex ring to clamp the tube wall of the tube body to achieve the purpose of sealing. The double sealing structure greatly enhances the sealing of the cryopreservation tube, effectively preventing liquid nitrogen or water from entering the tube and avoiding cell contamination.
[0014] 2. A water absorption ring is embedded in the tube body. If liquid nitrogen or water enters the gap between the tube body and the tube cover during cell recovery, the water absorption ring can absorb the infiltrated liquid. The detachable installation method facilitates the replacement of the water absorption ring, further improving the practicality of the cryopreservation tube. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.
[0016] Figure 1 It is a schematic diagram of the overall structure of the utility model.
[0017] Figure 2 It is a schematic diagram of the pipe cover structure of the present utility model.
[0018] Figure 3 It is an exploded view of the overall structure of the utility model.
[0019] Figure 4 This utility model Figure 1 Horizontal cross-sectional view.
[0020] Figure 5 This utility model Figure 4 Schematic diagram of the slider movement.
[0021] In the accompanying drawings, there are a tube body 1, a first thread 10, an annular groove 11, a tube cover 2, a second thread 20, an outer cover body 21, an inner cover body 22, an inner cavity channel 23, a horizontal channel 231, a vertical channel 232, a sealing assembly 24, a sealing ring 241, a sealing rod 242, a control rod 25, an elastic member 26, a reducing portion 251, a sliding buckle 27, a sliding groove 28, and a convex ring 29. DETAILED DESCRIPTION
[0022] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.
[0023] See also Figure 1-Figure 5 The utility model provides a cell freezing tube, comprising a tube body 1 and a tube cover 2 covering the tube body 1, the tube cover 2 comprising an outer cover 21 and an inner cover 22, the inner diameter of the outer cover 21 being larger than the outer diameter of the open end of the tube body 1, and the outer diameter of the inner cover 22 being smaller than the inner diameter of the open end of the tube body 1. Preferably, the minimum distance between the outer cover 21 and the inner cover 22 is equal to the thickness of the tube wall of the tube body 1. When covered, the inner cover 22 is inserted from the open end of the tube body 1, and the outer cover is wrapped around the outside of the open end of the tube body 1.
[0024] like Figure 4 and Figure 5As shown, the interior of the inner cover body 22 is provided with an inner cavity channel 23, and the inner cavity channel 23 includes a horizontal channel 231 and a vertical channel 232 arranged in the horizontal direction and the vertical direction respectively, and the horizontal channel 231 and the vertical channel 232 are connected; a sealing assembly 24 is provided in the horizontal channel 231, specifically, the horizontal channel 231 includes an annular channel and four straight channels, and the sealing assembly 24 includes a sealing ring 241 and four sealing rods 242, the sealing ring 241 is a semicircular annular structure, which is arranged in the annular channel, and the four sealing rods 242 are respectively arranged in the four straight channels, and one end of the sealing rod 242 is fixedly connected to the sealing ring 241, such as by bonding; a control rod 25 is provided in the vertical channel 232, and the middle outer peripheral surface of the control rod 25 is provided with a reducing portion 251, specifically, the reducing portion 251 can be made of elastic material, the top of the control rod 25 is a wedge-shaped inclined surface structure, and the bottom end of the control rod 25 is connected to the inner cover body 22 through an elastic member 26. The top of the pipe cover 2 is provided with a sliding buckle 27 and a sliding groove 28 connected to the vertical channel 232. The shape of the sliding groove 28 is adapted to the slider 261, which is arranged in the sliding groove 28 and is slidably connected to the pipe cover 2. Specifically, the inner cover body 22 and the sealing ring 241 are both made of rubber material, and the elastic member 26 and the second elastic member 263 are both springs.
[0025] like Figure 4 and Figure 5 As shown, when the slider 27 is on the right side, the top end of the control rod 25 extends into the slide groove along the vertical channel 232. At this time, the control rod 25 and the slider 27 do not abut against each other, and the diameter-reducing portion 251 and the horizontal channel 231 are not in the same horizontal plane. When the slider 27 slides from the right to the left side, the bottom end of the slider 27 abuts against the top end of the control rod 25, pressing the control rod 25 downward to move it vertically downward and compressing the elastic member 26. At this time, the diameter-reducing portion 251 and the horizontal channel 231 are in the same horizontal plane and abut against the four sealing rods 242, pushing the four sealing rods 242 to move outward. , which makes the diameter of the sealing ring 241 larger, and because the circumferential surface of the annular channel corresponding to the inner cover body 22 is thin-walled, the sealing ring 241 will be against the inner side wall of the annular channel after the diameter becomes larger, and the side wall is squeezed to a certain extent to bulge radially outward, so that the outer side wall of the annular channel is tightly against the outer circumferential surface of the tube body 1, achieving a tight sealing effect; when the slider 27 slides back from the left to the right, the elastic member 26 restores and drives the control rod 25 to move upward, and the diameter-reducing portion 251 is squeezed by the inner side wall of the vertical channel 232 and gradually moves away from the sealing rod 242, so that the sealing ring 241 is restored.
[0026] Based on the above scheme, in a further preferred embodiment, Figure 2 and Figure 4As shown, the inner circumference of the outer cover 21 is integrally formed with a semicircular raised ring 29, which is arranged on the same horizontal plane as the sealing ring 241. Specifically, the raised ring 29 can be made of an elastic material. In the sealed state, the relative force between the raised ring 29 and the sealing ring 241 of the inner cover 22 clamps the tube wall of the tube body 1 to enhance the sealing effect.
[0027] Based on the above scheme, in a further preferred embodiment, Figure 2 As shown, the inner circumference of the outer cover 21 is further provided with a second thread 20, and the outer circumference of the tube body 1 is provided with a first thread 10 that matches the second thread 20. By providing the thread, when closing the tube, the tube cover 2 and the tube body 1 are first threadedly connected, and then the slider 27 is slid and pressed to secure, further improving the sealing performance of the cryopreservation tube.
[0028] Based on the above scheme, in a further preferred embodiment, Figure 3 and Figure 4 As shown, an annular groove 11 is provided at the top of the tube body 1, and a water absorption ring 3 is detachably embedded in the annular groove 11. Specifically, the water absorption ring 3 is made of a highly absorbent material. When water enters the tube cover during the recovery process of the cell cryopreservation tube, the water absorption ring 3 can absorb the liquid, effectively preventing liquid nitrogen or water from entering the tube body 1.
[0029] As mentioned above, the tube body and the tube cover are tightly connected by threads, and the sealing assembly is driven to move by the slide buckle and the control rod, so that the sealing ring presses the side wall of the inner cover body and then presses against the inner side of the tube body, and cooperates with the convex ring to clamp the tube wall of the tube body to achieve the purpose of sealing. The double sealing structure greatly enhances the sealing of the cryopreservation tube, effectively preventing liquid nitrogen or water from entering the tube and avoiding cell contamination; a water absorption ring is embedded in the tube body. If liquid nitrogen or water enters from the gap between the tube body and the tube cover during the cell recovery process, the infiltrated liquid can be absorbed by the water absorption ring. The detachable installation method facilitates the replacement of the water absorption ring, further improving the practicality of the cryopreservation tube.
[0030] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate the description of this utility model and simplify the description. They do not indicate or imply that the devices or components referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.
[0031] Obviously, the above-described embodiments of the present invention are merely examples for the purpose of clearly illustrating the present invention and are not intended to limit the manner in which the present invention is to be implemented. A person skilled in the art would be able to make various obvious changes, readjustments, and substitutions without departing from the scope of protection of the present invention. It is not necessary and impossible to enumerate all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the claims of the present invention.
Claims
1. A cell freezing tube, comprising a tube body (1) and a tube cover (2) covering the tube body (1), characterized in that: The tube cover (2) comprises an outer cover body (21) and an inner cover body (22), wherein the inner diameter of the outer cover body (21) is larger than the outer diameter of the open end of the tube body (1), and the outer diameter of the inner cover body (22) is smaller than the inner diameter of the open end of the tube body (1); an inner cavity channel (23) is provided inside the inner cover body (22), wherein the inner cavity channel (23) comprises a horizontal channel (231) and a vertical channel (232) which are connected to each other, and a sealing component (24) is movably connected inside the horizontal channel (231), wherein the sealing component (24) comprises a sealing ring (241) and a sealing rod (232). 42), one end of the sealing rod (242) is fixedly connected to the sealing ring (241), a control rod (25) is provided in the vertical channel (232), one end of the control rod (25) is movably connected to the inner cover body (22) through an elastic member (26), and a diameter reducing portion (251) is provided on the outer peripheral surface of the middle part of the control rod (25); a sliding buckle (27) is provided on the top of the pipe cover (2), and the sliding buckle (27) is slidably connected to the pipe cover (2), and the sliding buckle (27) can be moved to make the bottom end of the sliding buckle (27) abut against or separate from the other end of the control rod (25).
2. A cell freezing tube according to claim 1, characterized in that: A sliding groove (28) adapted to the sliding buckle (27) is provided on the top of the tube cover (2), and the sliding buckle (27) is arranged in the sliding groove (28).
3. A cell freezing tube according to claim 1, characterized in that: The inner circumferential surface of the outer cover (21) is provided with a convex ring (29), and the convex ring (29) and the sealing ring (241) are arranged on the same horizontal plane.
4. A cell freezing tube according to claim 1, characterized in that: The inner circumference of the outer cover (21) is provided with a second thread (20), and the outer circumference of the tube (1) is provided with a first thread (10) that matches the second thread (20).
5. The cell freezing tube according to claim 1, characterized in that: The inner cover (22) and the sealing ring are both made of rubber material.
6. A cell freezing tube according to claim 1, characterized in that: An annular groove (11) is provided on the top of the tube body (1), and a water absorption ring (3) is detachably embedded in the annular groove (11).
7. A cell freezing tube according to claim 6, characterized in that: The water absorption ring (3) is made of highly water-absorbent material.